CN101501207B - 合成基因组 - Google Patents
合成基因组 Download PDFInfo
- Publication number
- CN101501207B CN101501207B CN200680052444.3A CN200680052444A CN101501207B CN 101501207 B CN101501207 B CN 101501207B CN 200680052444 A CN200680052444 A CN 200680052444A CN 101501207 B CN101501207 B CN 101501207B
- Authority
- CN
- China
- Prior art keywords
- expression cassette
- genome
- synthetic
- gene group
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 claims abstract description 101
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 39
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 38
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 38
- 230000001580 bacterial effect Effects 0.000 claims abstract description 5
- 108700005078 Synthetic Genes Proteins 0.000 claims description 61
- 108091034117 Oligonucleotide Proteins 0.000 claims description 25
- 239000002773 nucleotide Substances 0.000 claims description 13
- 125000003729 nucleotide group Chemical group 0.000 claims description 13
- 238000000338 in vitro Methods 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- 230000006798 recombination Effects 0.000 claims description 5
- 238000005215 recombination Methods 0.000 claims description 5
- 238000010367 cloning Methods 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 86
- 239000000592 Artificial Cell Substances 0.000 abstract description 24
- 238000013461 design Methods 0.000 abstract description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 6
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 abstract description 3
- 229910052739 hydrogen Inorganic materials 0.000 abstract description 3
- 239000001257 hydrogen Substances 0.000 abstract description 3
- 239000000446 fuel Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 50
- 241000204031 Mycoplasma Species 0.000 description 22
- 239000000047 product Substances 0.000 description 18
- 241000204051 Mycoplasma genitalium Species 0.000 description 11
- 244000005700 microbiome Species 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 8
- 108020004414 DNA Proteins 0.000 description 8
- 230000001413 cellular effect Effects 0.000 description 8
- 230000008859 change Effects 0.000 description 8
- 210000002220 organoid Anatomy 0.000 description 8
- 239000012634 fragment Substances 0.000 description 7
- 150000002632 lipids Chemical class 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 235000016709 nutrition Nutrition 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- 238000012937 correction Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000013519 translation Methods 0.000 description 5
- 241000202936 Mycoplasma mycoides Species 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- CWHJIJJSDGEHNS-MYLFLSLOSA-N Senegenin Chemical compound C1[C@H](O)[C@H](O)[C@@](C)(C(O)=O)[C@@H]2CC[C@@]3(C)C(CC[C@]4(CCC(C[C@H]44)(C)C)C(O)=O)=C4[C@@H](CCl)C[C@@H]3[C@]21C CWHJIJJSDGEHNS-MYLFLSLOSA-N 0.000 description 4
- CJPQIRJHIZUAQP-MRXNPFEDSA-N benalaxyl-M Chemical compound CC=1C=CC=C(C)C=1N([C@H](C)C(=O)OC)C(=O)CC1=CC=CC=C1 CJPQIRJHIZUAQP-MRXNPFEDSA-N 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 230000002438 mitochondrial effect Effects 0.000 description 4
- 108091033319 polynucleotide Proteins 0.000 description 4
- 102000040430 polynucleotide Human genes 0.000 description 4
- 239000002157 polynucleotide Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 230000010076 replication Effects 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 239000009871 tenuigenin Substances 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 3
- 206010035664 Pneumonia Diseases 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000008034 disappearance Effects 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 238000002703 mutagenesis Methods 0.000 description 3
- 231100000350 mutagenesis Toxicity 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 241001430197 Mollicutes Species 0.000 description 2
- 241000202956 Mycoplasma arthritidis Species 0.000 description 2
- 241000204003 Mycoplasmatales Species 0.000 description 2
- 108091092724 Noncoding DNA Proteins 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 241000935255 Ureaplasma parvum Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000002551 biofuel Substances 0.000 description 2
- 238000012938 design process Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000002513 implantation Methods 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 239000002502 liposome Substances 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 210000003463 organelle Anatomy 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 230000003685 thermal hair damage Effects 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 241000203024 Acholeplasma Species 0.000 description 1
- 241001114404 Acholeplasmatales Species 0.000 description 1
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241001673276 Candidatus Phytoplasma asteris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 238000000018 DNA microarray Methods 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241001114405 Entomoplasmatales Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000204025 Mycoplasma capricolum Species 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 108010010677 Phosphodiesterase I Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 108700005075 Regulator Genes Proteins 0.000 description 1
- 108010000605 Ribosomal Proteins Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000002146 bilateral effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000012854 evaluation process Methods 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N ferric oxide Chemical compound O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 230000004992 fission Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000011207 functional examination Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000126 in silico method Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000006180 nutrition needs Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 150000003016 phosphoric acids Chemical class 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 210000002427 ring chromosome Anatomy 0.000 description 1
- -1 rrna Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 108010068698 spleen exonuclease Proteins 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US74254205P | 2005-12-06 | 2005-12-06 | |
| US60/742,542 | 2005-12-06 | ||
| PCT/US2006/046803 WO2008024129A2 (en) | 2005-12-06 | 2006-12-06 | Synthetic genomes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101501207A CN101501207A (zh) | 2009-08-05 |
| CN101501207B true CN101501207B (zh) | 2014-03-12 |
Family
ID=39107256
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200680052444.3A Active CN101501207B (zh) | 2005-12-06 | 2006-12-06 | 合成基因组 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US10041060B2 (enExample) |
| EP (1) | EP1968994B1 (enExample) |
| JP (1) | JP5106412B2 (enExample) |
| CN (1) | CN101501207B (enExample) |
| AU (1) | AU2006347573B2 (enExample) |
| CA (1) | CA2643356A1 (enExample) |
| DK (1) | DK1968994T3 (enExample) |
| IL (1) | IL192041A (enExample) |
| WO (1) | WO2008024129A2 (enExample) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK1915446T3 (en) * | 2005-08-11 | 2017-09-11 | Synthetic Genomics Inc | IN VITRO RECOMBINATION PROCEDURE |
| US20090017513A1 (en) * | 2007-07-13 | 2009-01-15 | Georgia Belle Plantation, Inc. | Process for producing hydrocarbon molecules from renewable biomass |
| WO2010102257A2 (en) * | 2009-03-06 | 2010-09-10 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
| US9267132B2 (en) | 2007-10-08 | 2016-02-23 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
| DK2255013T3 (en) * | 2008-02-15 | 2016-09-12 | Synthetic Genomics Inc | Methods for in vitro joining and combinatorial assembly of nucleic acid molecules. |
| US10093552B2 (en) | 2008-02-22 | 2018-10-09 | James Weifu Lee | Photovoltaic panel-interfaced solar-greenhouse distillation systems |
| US9259662B2 (en) | 2008-02-22 | 2016-02-16 | James Weifu Lee | Photovoltaic panel-interfaced solar-greenhouse distillation systems |
| AU2009217293B2 (en) * | 2008-02-23 | 2014-11-20 | James Weifu Lee | Designer organisms for photobiological butanol production from carbon dioxide and water |
| US8986963B2 (en) * | 2008-02-23 | 2015-03-24 | James Weifu Lee | Designer calvin-cycle-channeled production of butanol and related higher alcohols |
| CN102015995B (zh) | 2008-03-03 | 2014-10-22 | 焦耳无限科技公司 | 产生碳基目的产物的二氧化碳固定工程微生物 |
| EP2998402A1 (en) | 2008-10-17 | 2016-03-23 | Joule Unlimited Technologies, Inc. | Ethanol production by microorganisms |
| EP3257942B1 (en) | 2008-12-18 | 2020-07-22 | ITI Scotland Limited | Method for assembly of polynucleic acid sequences |
| US7794969B1 (en) | 2009-07-09 | 2010-09-14 | Joule Unlimited, Inc. | Methods and compositions for the recombinant biosynthesis of n-alkanes |
| US20120077251A1 (en) | 2010-09-14 | 2012-03-29 | C/O Joule Unlimited Technologies, Inc. | Methods and Compositions for Targeting Heterologous Integral Membrane Proteins to the Cyanobacterial Plasma Membrane |
| WO2011038134A1 (en) | 2009-09-25 | 2011-03-31 | Ls9, Inc. | Production of fatty acid derivatives |
| US20110269119A1 (en) | 2009-10-30 | 2011-11-03 | Synthetic Genomics, Inc. | Encoding text into nucleic acid sequences |
| MX2012005069A (es) * | 2009-10-30 | 2012-07-17 | Synthetic Genomics Inc | Codificar texto hacia secuencias de acido nucleico. |
| CN102939380A (zh) * | 2010-03-05 | 2013-02-20 | 合成基因组股份有限公司 | 用于克隆和操作基因组的方法 |
| GB2481425A (en) | 2010-06-23 | 2011-12-28 | Iti Scotland Ltd | Method and device for assembling polynucleic acid sequences |
| US8349587B2 (en) | 2011-10-31 | 2013-01-08 | Ginkgo Bioworks, Inc. | Methods and systems for chemoautotrophic production of organic compounds |
| US8790901B2 (en) | 2011-12-14 | 2014-07-29 | Pronutria, Inc. | Microorganisms and methods for producing unsaturated fatty acids |
| HK1206765A1 (en) | 2012-03-26 | 2016-01-15 | Axcella Health Inc. | Nutritive fragments, proteins and methods |
| SG10201604464SA (en) | 2012-03-26 | 2016-07-28 | Axcella Health Inc | Charged nutritive proteins and methods |
| HK1206764A1 (en) | 2012-03-26 | 2016-01-15 | Axcella Health Inc. | Nutritive proteins and methods |
| EP2831102A4 (en) | 2012-03-26 | 2015-12-02 | Pronutria Inc | NUTRITIONAL FRAGMENTS, PROTEINS THEREFOR AND METHOD |
| GB201219989D0 (en) | 2012-11-06 | 2012-12-19 | Discuva Ltd | Bacterial engineering |
| WO2014089436A1 (en) | 2012-12-07 | 2014-06-12 | Ginkgo Bioworks, Inc. | Methods and systems for methylotrophic production of organic compounds |
| US11408013B2 (en) | 2013-07-19 | 2022-08-09 | Cargill, Incorporated | Microorganisms and methods for the production of fatty acids and fatty acid derived products |
| WO2015048334A2 (en) | 2013-09-25 | 2015-04-02 | Pronutria, Inc. | Edible species nutritive polypeptides and methods of production and use thereof |
| WO2015048339A2 (en) | 2013-09-25 | 2015-04-02 | Pronutria, Inc. | Compositions and formulations for non-human nutrition and methods of production and use thereof |
| WO2015054507A1 (en) | 2013-10-10 | 2015-04-16 | Pronutria, Inc. | Nutritive polypeptide production systems, and methods of manufacture and use thereof |
| GB201413202D0 (en) | 2014-07-25 | 2014-09-10 | Discuva Ltd | Process for producing bacterial mutants |
| EP3430133A1 (en) | 2016-03-17 | 2019-01-23 | INVISTA Textiles (U.K.) Limited | Carboxylic acid reductase polypeptides and variants having improved activity, materials and processes relating thereto |
| US11085037B2 (en) * | 2016-03-23 | 2021-08-10 | Codex Dna, Inc. | Generation of synthetic genomes |
| CN110494566A (zh) | 2017-02-02 | 2019-11-22 | 嘉吉公司 | 产生c6-c10脂肪酸衍生物的经遗传修饰的细胞 |
| EP3775182A1 (en) | 2018-03-30 | 2021-02-17 | INVISTA Textiles (U.K.) Limited | Materials and methods for biosynthetic manufacture of pimelic acid and utilization of synthetic polypeptides |
| EP3775242A1 (en) | 2018-03-30 | 2021-02-17 | INVISTA Textiles (U.K.) Limited | High hydrogen utilization and gas recycle |
| EP3775240A1 (en) | 2018-03-30 | 2021-02-17 | INVISTA Textiles (U.K.) Limited | Method for controlling dissolved oxygen concentration in a continuous aerobic fermentation |
| US11512276B2 (en) | 2018-03-30 | 2022-11-29 | Inv Nylon Chemicals Americas, Llc | Methods for controlling oxygen concentration during aerobic biosynthesis |
| WO2019213033A1 (en) | 2018-05-02 | 2019-11-07 | Invista North America S.A.R.L. | Materials and methods for maximizing biosynthesis through alteration of pyruvate-acetyl-coa-tca balance in species of the genera ralstonia and cupriavidus and organisms related thereto |
| EP3788156A1 (en) | 2018-05-02 | 2021-03-10 | INVISTA Textiles (U.K.) Limited | Methods for controlling limitation conditions in product biosynthesis for non-phb generating cupriavidus or ralstonia |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6521427B1 (en) * | 1997-09-16 | 2003-02-18 | Egea Biosciences, Inc. | Method for the complete chemical synthesis and assembly of genes and genomes |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5750380A (en) | 1981-06-30 | 1998-05-12 | City Of Hope Research Institute | DNA polymerase mediated synthesis of double stranded nucleic acids |
| US5928905A (en) | 1995-04-18 | 1999-07-27 | Glaxo Group Limited | End-complementary polymerase reaction |
| US5976846A (en) | 1996-01-13 | 1999-11-02 | Passmore; Steven E. | Method for multifragment in vivo cloning and mutation mapping |
| IL120338A0 (en) * | 1997-02-27 | 1997-06-10 | Gesher Israel Advanced Biotecs | Single step DNA fragments assembly |
| US6670127B2 (en) * | 1997-09-16 | 2003-12-30 | Egea Biosciences, Inc. | Method for assembly of a polynucleotide encoding a target polypeptide |
| JP3131633B1 (ja) | 1999-11-26 | 2001-02-05 | 農林水産省食品総合研究所長 | Pcr法による植物遺伝子の検出方法 |
| CN1384203A (zh) | 2001-04-30 | 2002-12-11 | 香港科技创业股份有限公司 | 具有高度延伸专一性的dna低温循环延伸反应方法 |
| US7267984B2 (en) * | 2002-10-31 | 2007-09-11 | Rice University | Recombination assembly of large DNA fragments |
| US20040185449A1 (en) | 2003-03-20 | 2004-09-23 | Quinn John J. | Method for preparing assay samples |
| EP1629097A1 (en) * | 2003-05-22 | 2006-03-01 | University of California | Method for producing a synthetic gene or other dna sequence |
| KR100624452B1 (ko) | 2004-12-21 | 2006-09-18 | 삼성전자주식회사 | 고정화된 히드로겔 또는 peg-히드로겔 공중합체를이용한 핵산의 분리 및 정제 방법 |
| DK1915446T3 (en) | 2005-08-11 | 2017-09-11 | Synthetic Genomics Inc | IN VITRO RECOMBINATION PROCEDURE |
| DK1929012T3 (da) | 2005-08-11 | 2011-01-31 | Synthetic Genomics Inc | Fremgangsmåde til in vitro-rekombination |
| US20090305233A1 (en) | 2007-07-03 | 2009-12-10 | Arizona Board Of Regents, A Body Corporate Of The State Of Arizona | Methods and Reagents for Polynucleotide Assembly |
| DK2255013T3 (en) | 2008-02-15 | 2016-09-12 | Synthetic Genomics Inc | Methods for in vitro joining and combinatorial assembly of nucleic acid molecules. |
| WO2010042007A1 (en) | 2008-10-10 | 2010-04-15 | Jonas Tegenfeldt | Method for the mapping of the local at/gc ratio along dna |
| SG10201701022VA (en) | 2012-08-16 | 2017-04-27 | Synthetic Genomics Inc | Digital to biological converter |
| WO2014093535A1 (en) | 2012-12-13 | 2014-06-19 | Synthetic Genomics, Inc. | Peg-mediated assembly of nucleic acid molecules |
-
2006
- 2006-12-06 WO PCT/US2006/046803 patent/WO2008024129A2/en not_active Ceased
- 2006-12-06 EP EP06851474.4A patent/EP1968994B1/en active Active
- 2006-12-06 DK DK06851474.4T patent/DK1968994T3/da active
- 2006-12-06 CN CN200680052444.3A patent/CN101501207B/zh active Active
- 2006-12-06 CA CA002643356A patent/CA2643356A1/en not_active Abandoned
- 2006-12-06 US US11/635,355 patent/US10041060B2/en active Active
- 2006-12-06 AU AU2006347573A patent/AU2006347573B2/en active Active
- 2006-12-06 JP JP2008544524A patent/JP5106412B2/ja active Active
-
2008
- 2008-06-10 IL IL192041A patent/IL192041A/en active IP Right Grant
-
2018
- 2018-08-06 US US16/056,343 patent/US20180340165A1/en not_active Abandoned
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6521427B1 (en) * | 1997-09-16 | 2003-02-18 | Egea Biosciences, Inc. | Method for the complete chemical synthesis and assembly of genes and genomes |
Non-Patent Citations (11)
| Title |
|---|
| Accurate multiplex gene synthesis from programmable DNA microchips;Jingdong Tian,et al.;《Nature》;20041230;第432卷;1050-1054 * |
| Clyde A. Hutchison III,et al..Global Transposon Mutagenesis and a Minimal Mycoplasma Genome.《Science》.1999,第286卷2165-2169. |
| ELIOT MARSHALL.Venter Gets Down to Life’s Basics.《Science》.2002,第298卷1701. |
| ELIZABETH PENNISI.Venter Cooks Up a Synthetic Genome in Record Time.《Science》.2003,第302卷1307. |
| Generating a synthetic genome by whole genome assembly:X174 bacteriophage from synthetic;Hamilton O. Smith,et al.;《PNAS》;20031223;第100卷(第26期);15440-15445 * |
| Global Transposon Mutagenesis and a Minimal Mycoplasma Genome;Clyde A. Hutchison III,et al.;《Science》;19991210;第286卷;2165-2169 * |
| Hamilton O. Smith,et al..Generating a synthetic genome by whole genome assembly: |
| Jingdong Tian,et al..Accurate multiplex gene synthesis from programmable DNA microchips.《Nature》.2004,第432卷1050-1054. |
| Venter Cooks Up a Synthetic Genome in Record Time;ELIZABETH PENNISI;《Science》;20031121;第302卷;1307 * |
| Venter Gets Down to Life’s Basics;ELIOT MARSHALL;《Science》;20021129;第298卷;1701 * |
| X174 bacteriophage from synthetic.《PNAS》.2003,第100卷(第26期),15440-15445. |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1968994B1 (en) | 2013-07-03 |
| EP1968994A2 (en) | 2008-09-17 |
| IL192041A0 (en) | 2008-12-29 |
| WO2008024129A2 (en) | 2008-02-28 |
| US20070264688A1 (en) | 2007-11-15 |
| US10041060B2 (en) | 2018-08-07 |
| JP5106412B2 (ja) | 2012-12-26 |
| US20180340165A1 (en) | 2018-11-29 |
| JP2009518038A (ja) | 2009-05-07 |
| DK1968994T3 (da) | 2013-09-30 |
| CN101501207A (zh) | 2009-08-05 |
| CA2643356A1 (en) | 2008-02-28 |
| AU2006347573B2 (en) | 2013-01-17 |
| EP1968994A4 (en) | 2009-04-08 |
| WO2008024129A3 (en) | 2008-10-09 |
| AU2006347573A1 (en) | 2008-02-28 |
| IL192041A (en) | 2013-08-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101501207B (zh) | 合成基因组 | |
| Glick et al. | Molecular biotechnology: principles and applications of recombinant DNA | |
| Gallaher et al. | High‐throughput sequencing of the chloroplast and mitochondrion of Chlamydomonas reinhardtii to generate improved de novo assemblies, analyze expression patterns and transcript speciation, and evaluate diversity among laboratory strains and wild isolates | |
| Al-Haj et al. | Cyanobacteria as chassis for industrial biotechnology: progress and prospects | |
| Moya et al. | Toward minimal bacterial cells: evolution vs. design | |
| Ran et al. | Genome erosion in a nitrogen-fixing vertically transmitted endosymbiotic multicellular cyanobacterium | |
| Robart et al. | Group II intron retroelements: function and diversity | |
| Bishé et al. | Modification of RSF1010-based broad-host-range plasmids for improved conjugation and cyanobacterial bioprospecting | |
| Su et al. | RNA-Seq analyses reveal the order of tRNA processing events and the maturation of C/D box and CRISPR RNAs in the hyperthermophile Methanopyrus kandleri | |
| CN109072243A (zh) | 通过crispr-cas系统进行的分子记录的方法和系统 | |
| Zhou et al. | Strategies and methods for improving the efficiency of CRISPR/Cas9 gene editing in plant molecular breeding | |
| Jovicevic et al. | Total synthesis of a eukaryotic chromosome: Redesigning and SCRaMbLE‐ing yeast | |
| Teufel et al. | A multifunctional system for genome editing and large-scale interspecies gene transfer | |
| Wang et al. | Guide RNA scaffold variants enabled easy cloning of large gRNA cluster for multiplexed gene editing | |
| Permyakova et al. | CRISPR/Cas9-mediated targeted DNA integration: rearrangements at the junction of plant and plasmid DNA | |
| Walker et al. | Design and assembly of the 117-kb Phaeodactylum tricornutum chloroplast genome | |
| Reisinger et al. | Total synthesis of multi-kilobase DNA sequences from oligonucleotides | |
| Chakrabarti et al. | De novo transcriptome assembly and dynamic spatial gene expression analysis in red clover | |
| Gali et al. | Development of a sequence-based reference physical map of pea (Pisum sativum L.) | |
| Ohtani et al. | Serial assembly of Thermus megaplasmid DNA in the genome of Bacillus subtilis 168: a BAC‐based domino method applied to DNA with a high GC content | |
| Shaposhnikov et al. | Genome-Editing Tools for Lactic Acid Bacteria: Past Achievements, Current Platforms, and Future Directions | |
| Kong et al. | Combination of hairy root and whole-plant transformation protocols to achieve efficient CRISPR/Cas9 genome editing in soybean | |
| Tang et al. | Genome editing in rice and tomato with a small Un1Cas12f1 nuclease | |
| Karas et al. | Rescue of mutant fitness defects using in vitro reconstituted designer transposons in Mycoplasma mycoides | |
| Kosariya et al. | Strategies for strain improvement of economically important microorganisms |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: SYNTHETIC GENOMICS LTD. Free format text: FORMER OWNER: JOHN CRAIG VENTER RESEARCH CO., LTD. Effective date: 20100512 |
|
| CB03 | Change of inventor or designer information |
Inventor after: Smith Hamilton O. Inventor after: Smith Hamilton O. Inventor after: Hutchison Clyde A. III Inventor before: Smith Hamilton O. Inventor before: Smith Hamilton O. |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: VENTER CRAIG J. SMITH HAMILTON O. TO: VENTER CRAIG J. SMITH HAMILTON O. HUTCHISON CLYDE A. III |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20100512 Address after: American California Applicant after: Synthetic Genomics Inc. Address before: American California Applicant before: J. Craig. Venter research, Limited by Share Ltd |
|
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Smith Hamilton O. Inventor after: Smith Hamilton O. Inventor after: Hutchison Clyde A. III Inventor after: Young Lei Inventor before: Smith Hamilton O. Inventor before: Smith Hamilton O. Inventor before: Hutchison Clyde A. III |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: J. CRAIG VENTER HAMILTON O. SMITH CLYDE A. HUTCHISON III TO: J. CRAIG VENTER HAMILTON O. SMITH CLYDE A. HUTCHISON III DANIEL G. GIBSON |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |