CN101497642A

CN101497642A - Method for directly extracting tea saponin from tea seeds

Info

Publication number: CN101497642A
Application number: CNA2009100379873A
Authority: CN
Inventors: 张卫国
Original assignee: Shaoguan University
Current assignee: Shaoguan University
Priority date: 2009-03-19
Filing date: 2009-03-19
Publication date: 2009-08-05

Abstract

The invention relates to a method for extracting tea saponin directly from tea seeds. The method comprises the following steps: firstly, the oil-tea camellia seeds are hulled, tea kernels are crushed into 200-300 meshes of particles; then, the leaching is carried out, the PH value of the leaching solution is controlled between 5 and 9, the leaching solution is added with organic solution, the amount of the organic solution is determined according to the weight-to-volume ratio (3-8 times) between the oil-tea camellia seeds and the organic solution, the temperature is controlled between 60-80 DEG C, the extraction time is 1-4 hours, and the lixiviation is repeatedly performed for 1-2 times; then, the separation and the solvent recovery are carried out, the filtering or the centrifugal separation is performed to the leaching solution, and meanwhile, the reduced pressure evaporation and the recovery are performed to the organic solvent in the leaching solution to obtain aqueous solution of the tea saponin; and finally, the water is removed from the liquid tea saponin to obtain the crude tea saponin product. The invention has the advantages that the oil-tea camellia seeds are directly taken as the raw materials for extracting the tea saponin, the extract of the obtained tea saponin has high purity and good quality, and the method is suitable for the industrial large-scale production.

Description

Directly from the tea seed, extract the method for tea saponin

[technical field]

The invention belongs to farming, secondary product of forestry deep process technology field, relate to a kind of method of directly from the tea seed, extracting tea saponin.

[background technology]

Oil tea is that Camellia oleifera Abel is the camellia plant, and main product China concentrates to be distributed in the south China and the west and south.According to incompletely statistics, China produces more than 60 ten thousand tons in oil tea seed per year, produces tea oil about 150,000 tons and about 500,000 tons tea seed grouts per year.The tea saponin that contains 10-20% in the tea seed grouts.Tea saponin is a kind of five rings three obedient class materials, the sugared body of possess hydrophilic property and hydrophobic sapogenin, it is a kind of natural nonionic surface active agent, have pharmacological functions such as surfactivity effects such as emulsification, dispersion, moistening, foaming and impervious, anti-inflammatory, analgesia, and the function that sterilizing pesticide is arranged and stimulate certain plants to grow, thereby be widely used in daily use chemicals, agricultural chemicals, food and medicine and other fields.The tea saponin good water solubility, foam is abundant, and strong detergency is that articles for washing industry, the splendid of emulsifying agent industry are selected materials, in order to producing shampoo, body wash, shampoo, not only hair-washing hair-care, but also anti-inflammatory anti-itch, its anti-dandruff is effective.The anti-leakage of tea saponin and anti-inflammatory feature pharmaceutically have anti-inflammatory, analgesia, antibiotic, relieving cough and reducing sputum effect, and highly purified tea saponin can be developed into national two kind new medicine tea saponin capsules.Aspect prawn and crab cultivation, utilize the hemolytic action and the ichthyotoxin effect of tea saponin, can be used as pond agent clearly, kill harmful fish, and prawn and crab toxicological harmless effect.As one of main batching of novel low toxicity highly effective pesticide; with it is that the refining environmental protection type agricultural chemical auxiliary agent that forms of main body can be widely used in sterilant, sterilant, weedicide; simultaneously because the repellent and the bio-hormone effect of tea saponin; it itself also is a kind of good biological pesticide; can also stimulate plant growth; can not cause soil pollution, help environment protection.

At present existing many pieces of articles and patent disclosure be the method that raw material extracts tea saponin with the tea seed grouts, mainly contain water extraction and organic solvent extraction, though these technologies have obtained bigger progress, the tea saponin quality product that obtains can't satisfy the requirement in market.The tea saponin quality product is not only relevant with extracting method, also relevant with the raw material quality that extracts tea saponin, with the tea seed grouts behind the extraction tea oil is that raw material extraction tea saponin brings disadvantageous effect can for quality product (color and luster and purity etc.), dark as color and luster, purity is lower, therefore cause product postorder processed complex, carry out that impurity elimination and decolouring are handled etc. as needs, this will have influence on the activity that tea saponin itself had and the yield of product.

[summary of the invention]

In order to overcome the above-mentioned shortcoming of prior art, the invention provides a kind ofly have that technology is simple, production cost is low, product purity is high, the method for directly from the tea seed, extracting tea saponin of the good advantage of quality.

The technical solution adopted for the present invention to solve the technical problems is: a kind of method of directly from the tea seed, extracting tea saponin, earlier tea seed is shelled, and again through fragmentation, above-mentioned tea seed kernel is broken into 20 orders-300 purpose particle; Leaching then, the pH value scope of leaching liquid is controlled between the 5-9, adds organic solution in leaching liquid, the add-on of organic solution is 3-8 times according to the weightmeasurement ratio of tea seed and organic solution, temperature is controlled between 60-80 ℃, and extraction time is 1-4 hour, repeats lixiviate 1-2 time; And then separate and solvent recuperation, filtering or the centrifugation vat liquor, reduction vaporization reclaims the organic solvent in the vat liquor simultaneously, obtains the tea saponin aqueous solution; From the liquid tea saponin, remove moisture at last, obtain thick tea saponin product.

Described tea seed particle size after cracking is 60 orders-120 orders.

Described organic solvent includes methyl alcohol, ethanol and acetone.

Described organic solution is to get by the volume ratio of organic solvent and water is mixed, and its concentration range is 50-90%.

The invention has the beneficial effects as follows: directly be that raw material extracts tea saponin with the tea seed, gained tea saponin extract purity height, quality is good; Preparation technology is simple, has saved removal of impurities, decolouring step, has shortened the production cycle, has reduced production cost, is suitable for commercial scale production.

[description of drawings]

Fig. 1 is a technical process block diagram of the present invention;

[embodiment]

The present invention is further described below in conjunction with drawings and Examples:

Referring to Fig. 1, a kind of method of directly from the tea seed, extracting tea saponin,, earlier tea seed is shelled, again through fragmentation, above-mentioned tea seed kernel is broken into 20 orders-300 purpose particle, preferred tea seed particle size after cracking is 60 orders-120 orders; Leaching then, the pH value scope of leaching liquid is controlled between the 5-9, in leaching liquid, add organic solution, organic solution is to get by the volume ratio of organic solvent and water is mixed, and its concentration range is 50-90%, and organic solvent includes methyl alcohol, ethanol and acetone, the add-on of organic solution be the weightmeasurement ratio according to tea seed and organic solution be 3-8 doubly, temperature is controlled between 60-80 ℃, and extraction time is 1-4 hour, repeats lixiviate 1-2 time; And then separate and solvent recuperation, filtering or the centrifugation vat liquor, reduction vaporization reclaims the organic solvent in the vat liquor simultaneously, obtains the tea saponin aqueous solution; From the liquid tea saponin, remove moisture at last, obtain thick tea saponin product.

Embodiment 1:

Take by weighing big arnotto caul-fat tea seed kernel 100 grams, wet crushing is crossed 80 orders, adds water and ethanol then, makes alcohol concn reach 80%, volumes of aqueous ethanol is 5 times of tea seed weight, stir then, the centrifugation ethanolic soln was extracted in 70 ℃ of heating 2 hours, repeat lixiviate 2 times and wash residue, merge vat liquor, reclaim the ethanol and the dry tea saponin aqueous solution, obtain thick tea saponin.

Embodiment 2:

Take by weighing big arnotto caul-fat tea seed kernel 100 grams, wet crushing is crossed 80 orders, adds water and ethanol then, makes alcohol concn reach 85%, volumes of aqueous ethanol is 7 times of tea seed weight, stir then, the centrifugation ethanolic soln was extracted in 70 ℃ of heating 2 hours, repeat lixiviate 2 times and wash residue, merge vat liquor, reclaim the ethanol and the dry tea saponin aqueous solution, obtain thick tea saponin.

Claims

1, a kind of method of directly extracting tea saponin from the tea seed is shelled tea seed earlier, again through fragmentation, it is characterized in that: above-mentioned tea seed kernel is broken into 20 orders-300 purpose particle; Leaching then, the pH value scope of leaching liquid is controlled between the 5-9, adds organic solution in leaching liquid, the add-on of organic solution is 3-8 times according to the weightmeasurement ratio of tea seed and organic solution, temperature is controlled between 60-80 ℃, and extraction time is 1-4 hour, repeats lixiviate 1-2 time; And then separate and solvent recuperation, filtering or the centrifugation vat liquor, reduction vaporization reclaims the organic solvent in the vat liquor simultaneously, obtains the tea saponin aqueous solution; From the liquid tea saponin, remove moisture at last, obtain thick tea saponin product.

2, the method for directly extracting tea saponin from the tea seed as claimed in claim 1, it is characterized in that: described tea seed particle size after cracking is 60 orders-120 orders.

3, the method for directly extracting tea saponin from the tea seed as claimed in claim 1, it is characterized in that: described organic solvent includes methyl alcohol, ethanol and acetone.

4, the method for directly extracting tea saponin from the tea seed as claimed in claim 1 is characterized in that: described organic solution is to get by the volume ratio of organic solvent and water is mixed, and its concentration range is 50-90%,