CN101484181B - 用于生产l-天门冬酰胺酶ⅱ的重组宿主 - Google Patents

用于生产l-天门冬酰胺酶ⅱ的重组宿主 Download PDF

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CN101484181B
CN101484181B CN2007800248607A CN200780024860A CN101484181B CN 101484181 B CN101484181 B CN 101484181B CN 2007800248607 A CN2007800248607 A CN 2007800248607A CN 200780024860 A CN200780024860 A CN 200780024860A CN 101484181 B CN101484181 B CN 101484181B
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asnase
chromosome
host cell
seq
subunit
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CN101484181A (zh
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戴维·R·菲尔普拉
王懋梁
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Schweer Intellectual Property Uk Ltd
Schweitzer Uk Intellectual Property Co ltd
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Enzon Pharmaceuticals Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/82—Asparaginase (3.5.1.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Molecular Biology (AREA)
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  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN2007800248607A 2006-06-30 2007-06-08 用于生产l-天门冬酰胺酶ⅱ的重组宿主 Active CN101484181B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US81781706P 2006-06-30 2006-06-30
US60/817,817 2006-06-30
PCT/US2007/070706 WO2008011234A2 (en) 2006-06-30 2007-06-08 Recombinant host for producing l-asparaginase ii

Publications (2)

Publication Number Publication Date
CN101484181A CN101484181A (zh) 2009-07-15
CN101484181B true CN101484181B (zh) 2012-12-26

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CN2007800248607A Active CN101484181B (zh) 2006-06-30 2007-06-08 用于生产l-天门冬酰胺酶ⅱ的重组宿主

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Country Link
US (2) US7807436B2 (https=)
EP (1) EP2046369B1 (https=)
JP (1) JP5308333B2 (https=)
KR (1) KR101408062B1 (https=)
CN (1) CN101484181B (https=)
AU (1) AU2007275535B2 (https=)
CA (1) CA2652718A1 (https=)
DK (1) DK2046369T3 (https=)
ES (1) ES2553329T3 (https=)
HU (1) HUE025738T2 (https=)
IL (1) IL195168A (https=)
MX (1) MX2009000284A (https=)
NZ (1) NZ572519A (https=)
PL (1) PL2046369T3 (https=)
PT (1) PT2046369E (https=)
TW (1) TWI427148B (https=)
WO (1) WO2008011234A2 (https=)

Families Citing this family (11)

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Publication number Priority date Publication date Assignee Title
BR102014000585B1 (pt) 2014-01-10 2021-01-12 Coppe/Ufrj-Instituto Alberto Luiz Coimbra De Pós-Graduação l-asparaginase recombinante de zymomonas
CN105802948B (zh) 2014-12-29 2020-06-09 江苏众红生物工程创药研究院有限公司 聚乙二醇定点修饰的门冬酰胺酶及其制备方法与应用
US10480014B2 (en) * 2015-06-15 2019-11-19 Ajou University Industry-Academic Cooperation Foundation Method for producing natural rubber by using recombinant microorganism
KR102783452B1 (ko) 2016-06-01 2025-03-19 세르비에 아이피 유케이 리미티드 폴리알킬렌 옥시드-아스파라기나제의 제형, 및 그의 제조 및 사용 방법
KR101837636B1 (ko) * 2016-06-14 2018-03-12 전남대학교산학협력단 코리네박테리움 재조합 균주를 이용한 아스파라기나제의 분비생산 방법
CN108795832B (zh) * 2017-04-26 2021-05-14 江苏恒瑞医药股份有限公司 一种内源l-门冬酰胺酶ii基因敲除的宿主菌、其制备方法及其应用
WO2019083794A1 (en) 2017-10-27 2019-05-02 Pfenex Inc. PROCESS FOR PRODUCING EAR ASPARAGINASE. RECOMBINED COLI
JP7784804B2 (ja) 2017-10-27 2025-12-12 フェネックス インク． 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法
US11364223B2 (en) 2018-12-13 2022-06-21 Huyabio International, Llc Sulcardine administration for treatment of acute atrial fibrillation
US20220227805A1 (en) 2020-12-23 2022-07-21 Jazz Pharmaceuticals Ireland Ltd. Methods of purifying charge-shielded fusion proteins
CN114854729B (zh) * 2021-02-03 2025-09-02 重庆派金生物科技有限公司 定向化学偶联门冬酰胺酶突变体及其制备方法和应用

Citations (1)

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CN1748790A (zh) * 2005-09-16 2006-03-22 北京双鹭药业股份有限公司 一种天门冬酰胺酶注射液、其制备方法及应用

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US5324844A (en) 1989-04-19 1994-06-28 Enzon, Inc. Active carbonates of polyalkylene oxides for modification of polypeptides
US5122614A (en) 1989-04-19 1992-06-16 Enzon, Inc. Active carbonates of polyalkylene oxides for modification of polypeptides
US5643575A (en) 1993-10-27 1997-07-01 Enzon, Inc. Non-antigenic branched polymer conjugates
US5919455A (en) 1993-10-27 1999-07-06 Enzon, Inc. Non-antigenic branched polymer conjugates
US5615460A (en) * 1994-06-06 1997-04-01 The Procter & Gamble Company Female component for refastenable fastening device having regions of differential extensibility
US6180095B1 (en) 1997-12-17 2001-01-30 Enzon, Inc. Polymeric prodrugs of amino- and hydroxyl-containing bioactive agents
US5965119A (en) 1997-12-30 1999-10-12 Enzon, Inc. Trialkyl-lock-facilitated polymeric prodrugs of amino-containing bioactive agents
US6958212B1 (en) 1999-02-01 2005-10-25 Eidgenossische Technische Hochschule Zurich Conjugate addition reactions for the controlled delivery of pharmaceutically active compounds
US7087229B2 (en) 2003-05-30 2006-08-08 Enzon Pharmaceuticals, Inc. Releasable polymeric conjugates based on aliphatic biodegradable linkers
US7122189B2 (en) 2002-08-13 2006-10-17 Enzon, Inc. Releasable polymeric conjugates based on aliphatic biodegradable linkers

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
CN1748790A (zh) * 2005-09-16 2006-03-22 北京双鹭药业股份有限公司 一种天门冬酰胺酶注射液、其制备方法及应用

Non-Patent Citations (4)

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Title
Amardeep Khushoo等.Extracellular expression and single step puriWcation of recombinantEscherichia coli L-asparaginase II.《protein expression and purification》.2004,第29－36页. *
YINGDA WANG等.Cloning and Expressionof L-Asparaginase Gene in Escherichia coli.《applied biochemistry and biology》.2001,第95卷(第2期),第93-101页. *
赖龙生.L2天冬酰胺酶Ⅱ基因工程菌的培养.《药　物　生　物　技　术》.1999,第6卷(第3期),第140－143页. *
韩照中等.L2天门冬酰胺酶II 的表达与纯化.《药 物 生 物 技 术》.2001,第8卷(第1期),第22-25页. *

Also Published As

Publication number Publication date
HUE025738T2 (en) 2016-04-28
KR20090033175A (ko) 2009-04-01
MX2009000284A (es) 2009-01-26
CN101484181A (zh) 2009-07-15
WO2008011234A2 (en) 2008-01-24
ES2553329T3 (es) 2015-12-07
TW200817518A (en) 2008-04-16
IL195168A (en) 2014-12-31
PT2046369E (pt) 2015-11-26
KR101408062B1 (ko) 2014-06-20
TWI427148B (zh) 2014-02-21
JP5308333B2 (ja) 2013-10-09
US7807436B2 (en) 2010-10-05
EP2046369A4 (en) 2010-04-21
US20080063632A1 (en) 2008-03-13
CA2652718A1 (en) 2008-01-24
NZ572519A (en) 2011-06-30
DK2046369T3 (en) 2015-11-30
AU2007275535B2 (en) 2012-08-16
AU2007275535A1 (en) 2008-01-24
JP2009542219A (ja) 2009-12-03
IL195168A0 (en) 2011-08-01
US20100273236A1 (en) 2010-10-28
EP2046369A2 (en) 2009-04-15
PL2046369T3 (pl) 2016-01-29
WO2008011234A3 (en) 2008-10-16
EP2046369B1 (en) 2015-08-12

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