CN101480123A - Method for cultivating aseptic seedling of Ferula sinkiangensis - Google Patents
Method for cultivating aseptic seedling of Ferula sinkiangensis Download PDFInfo
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- CN101480123A CN101480123A CNA2009101132242A CN200910113224A CN101480123A CN 101480123 A CN101480123 A CN 101480123A CN A2009101132242 A CNA2009101132242 A CN A2009101132242A CN 200910113224 A CN200910113224 A CN 200910113224A CN 101480123 A CN101480123 A CN 101480123A
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Abstract
The invention discloses a ferula seedling-raising method, in particulate relates to a ferula sinkiangensis aseptic seedling cultivation method which is realized mainly through the procedures of seed selection, disinfection and germination bed culture. Compared with the prior art, the invention has the advantages that the method breakthroughs the lamination of the traditional ferula seedling-raising method, greatly improves the sprouting rate of seeds, can realize the scale ferula seedling-raising, can especially cultivate the ferula sinkiangensis aseptic seedlings indoors, can provide the seedlings all the year round, can ensure the culture tissues younger and avoid the direct harm of the disinfectant to the young tissues, improves the success ratio, realizes the scale tissue culture rapid propagation system of the ferula sinkiangensis, effectively protects Sinkiang wild medical resources, maintains the diversity of organisms, provides stable and sustainable medicinal resources for market demands of the pharmaceuticals industry, especially for the Chinese traditional medicine and the ethnic medicines, and has significant value.
Description
Technical field:
The present invention relates to a kind of asafoetide seedling-cultivating method, particularly a kind of method for cultivating aseptic seedling of Ferula sinkiangensis.
Technical background:
Ferula sinkiangensis (Ferula sinkiangensis K.M.Shen) is the perennial once solid class ephemeral in early spring of Umbelliferae (Umbelliferae) Ferula (Ferula L.), it is the only domestic source of a plurality of ethnic group of China traditional national medicinal material and Chinese medicine asafoetide, record for going through an edition Pharmacopoeia of the People's Republic of China, have high medical value.But the Ferula sinkiangensis plant resources is in the state of gradually endangering at present, is difficult to satisfy the present Chinese medicine that enlarges day by day, the market demand of ethnic drug, therefore it is carried out artificial fast numerous very urgent with protection of resources.
Ferula sinkiangensis is the ephemeral in early spring; growth rapidly; add that growing environment is abominable; organize very easy aging; gathering the culture materials Best Times is difficult to grasp; therefore the cell culture probalility of success reduces greatly; the ways and means that adopts only limits to cultivate early spring at present; make the asafoetide work of growing seedlings limited; can not form scale, particularly there is the physiological dormancy phenomenon in the Ferula sinkiangensis seed in natural environment, adopts usual method to grow seedlings; germination rate; success rate is very low; particularly be difficult to turn out aseptic seedling, and adopt indoor cultivation to breed aseptic seedling of Ferula sinkiangensis, seedling not only can be provided at all seasons; and can guarantee to have tenderer cultured tissue and avoid the direct injury of disinfectant tender tissue; improve culture success ratio, and then can protect the Xinjiang Wild drug resource effectively, keep bio-diversity; be pharmaceutical sector, particularly Chinese medicine; the market demand of ethnic drug provides stable; the herb resource of sustainable use.
Therefore, a kind ofly can provide seedling at all seasons, and can guarantee to have tenderer cultured tissue and avoid the direct injury of disinfectant that improve the asafoetide seedling-cultivating method of culture success ratio, particularly a kind of method for cultivating aseptic seedling of Ferula sinkiangensis just arises at the historic moment to tender tissue.
Summary of the invention:
The purpose of this invention is to provide a kind of asafoetide seedling-cultivating method; particularly a kind of method for cultivating aseptic seedling of Ferula sinkiangensis; to realize the scale tissue-culturing rapid propagation system of Ferula sinkiangensis; satisfy the Chinese medicine that enlarges day by day at present, the market demand of ethnic drug; and then protect the Xinjiang Wild drug resource effectively; keep bio-diversity, be that the market demand of pharmaceutical sector, particularly Chinese medicine, ethnic drug provides herb resource stable, sustainable use.
The objective of the invention is to be achieved through the following technical solutions:
Seed selection: select seed clean, full, neat and consistent;
Sterilization: selected seed is immersed 50～80% alcohol disinfectings, 25～35s, and draining and inserting concentration of volume percent to the no dropping liquid phenomenon is 10～15% H
2O
2Solution soaking 10～20min drains to the no dropping liquid phenomenon totally with aseptic water washing, drains to the nothing situation of dripping.
H in the above-mentioned disinfectant program
2O
2Can be that 3～8% NaClO solution or weight percent concentration are that 0.8～1.2% HgCI solution is replaced also by weight percent concentration.
Putting bed cultivates: the seed after will sterilize is tiled in the aseptic germination bed, and thickness is preferably between the seed supreme stack situation down and is advisable, and keeping envionmental humidity is 25～75%, in the cultivation 7～60 days down of 2～20 ℃ of temperature;
The above-mentioned bed of putting is preferably following a kind of:
A. cancel the seed behind the poison, place on the germinating bed after the sterilization, under 2～15 ℃ of conditions, cultivated 20～60 days; Described temperature condition is preferably 4～10 ℃;
B. cancel the seed behind the poison, through the gibberellin (GA of concentration 800～1200mg/L
3) 15～30h that soaks seed in the solution, clean with aseptic water washing, place on the germinating bed after the sterilization, under 15～20 ℃ of conditions, cultivated 7～15 days.
Above-mentioned germinating bed is preferably a kind of in the filter paper bed that casting bed or at least one metafiltration paper constituted
Duration of germination is observed the record germination, gets final product when radicle is broken through seed 1/2 above length, and gained can be used as that next step carries out tissue culture, seedling is planted or moves the material of sanction etc.
The present invention has broken through the restriction that traditional asafoetide is grown seedlings; improved seed germination rate greatly; can realize the scale that asafoetide is grown seedlings; particularly can adopt the indoor cultivation aseptic seedling of Ferula sinkiangensis; seedling can be provided at all seasons; and can guarantee to have tenderer cultured tissue and avoid the direct injury of disinfectant tender tissue; improve culture success ratio; realize the scale tissue-culturing rapid propagation system of Ferula sinkiangensis; protect the Xinjiang Wild drug resource effectively, keep bio-diversity, be pharmaceutical sector; Chinese medicine particularly; the market demand of ethnic drug provides stable; the herb resource of sustainable use has great importance.
Embodiment:
Embodiment 1:
Select seed clean, full, neat and consistent, selected seed is immersed 70% alcohol disinfecting 25s, draining and inserting concentration of volume percent to the no dropping liquid is 10% H
2O
2Solution soaking 15min drains to the no dropping liquid totally with aseptic water washing, drains to the nothing situation of dripping.Drain to the no dropping liquid with the aseptic water washing of 2 times of amounts of seed weight 2 times, drain to the nothing situation of dripping, described germinating bed is a casting bed, earlier with casting bed 30min under 150 ℃ of steam, be cooled to 4～10 ℃, the seed behind the cancellation poison places on the described seed sprouting bed, cultivates 30 days under 4 ℃ of conditions, duration of germination is also observed the record germination at any time, can be used as when radicle is broken through seed 1/2 above length that next step carries out tissue culture, seedling is planted or moves the material of sanction etc.
Embodiment 2:
Select seed clean, full, neat and consistent, selected seed is immersed 50% alcohol disinfecting 35s, draining and inserting concentration of volume percent to the no dropping liquid phenomenon is 15% H
2O
2Solution soaking 10min, drain to the no dropping liquid phenomenon with 5 times of weight aseptic water washings 3, drain to the nothing situation of dripping, seed after will sterilizing on the casting bed after fully sterilizing is tiled in the aseptic germination bed, thickness is that supreme stack situation down is advisable between the seed, keeping envionmental humidity is 55%, cultivated 60 days down for 10 ℃ in temperature, duration of germination is observed the record germination, get final product when 70% above seed base-root is broken through seed 1/2 above length, the gained plumule can be used as that next step carries out tissue culture, seedling is planted or moves the material of sanction etc.
Embodiment 3:
Select seed clean, full, neat and consistent, selected seed is immersed 60% alcohol disinfecting 30s, draining and inserting concentration of volume percent to the no dropping liquid is 14% H
2O
2Solution soaking 14min drains to the no dropping liquid totally with aseptic water washing, drains to the nothing situation of dripping.Drain to the no dropping liquid with the aseptic water washing of 3 times of weight of seed 2 times, drain to the nothing situation of dripping, described germinating bed is a casting bed, earlier with casting bed at disinfection by ultraviolet light 60min, seed behind the cancellation poison places on the described seed sprouting bed, cultivates 30 days under 7 ℃ of conditions, and duration of germination is also observed the record germination at any time, when the radicle of seed over half is broken through seed 1/2 above length, can be used as promptly that next step carries out tissue culture, seedling is planted or move the material of sanction etc.
Embodiment 4:
Select seed clean, full, neat and consistent, selected seed is immersed 65% alcohol disinfecting 30s, draining and inserting concentration of volume percent to the no dropping liquid phenomenon is 11% H
2O
2Solution soaking 10min, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, 20h soaks seed in the Gibberellins solution of concentration 800mg/L, with 2 times of weight aseptic water washings 1 time, get the two-layer tiling of aseptic filter paper as germinating bed, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down between the seed, keep envionmental humidity 70%, cultivated 10 days and observe at any time the record germination down for 15 ℃ in temperature, get final product when the radicle of 60% above seed is broken through seed 2/3 above length, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 5:
Seed selection: select seed clean, full, neat and consistent, selected seed is immersed 75% alcohol disinfecting 25s, draining and inserting concentration of volume percent to the no dropping liquid phenomenon is 15% H
2O
2Solution soaking 15min, drain to the no dropping liquid phenomenon with the aseptic water washing of 2 times of weight 3 times, drain to the nothing situation of dripping, 15h soaks seed in gibberellin 0 solution of concentration 1000mg/L, with 3 times of weight aseptic water washings 2 times, get 3 layers of tiling of aseptic filter paper as germinating bed, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down between the seed, keep envionmental humidity 60%, cultivated 7 days and observe at any time the record germination down for 20 ℃ in temperature, get final product when the radicle of 50% above seed is broken through seed 1/2 above length, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 6:
Seed selection: select seed clean, full, neat and consistent, selected seed is immersed 55% alcohol disinfecting 35s, draining and inserting concentration of volume percent to the no dropping liquid phenomenon is 13% H
2O
2Solution soaking 12min, drain to the no dropping liquid phenomenon with the aseptic water washing of 3 times of weight 3 times, drain to the nothing situation of dripping, seed behind the cancellation poison, 30h soaks seed in the Gibberellins solution of concentration 1100mg/L, with 3 times of weight aseptic water washings 2 times, get 2 layers of tiling of aseptic filter paper as germinating bed, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down between the seed, keep envionmental humidity 55%, cultivated 10 days and observe at any time the record germination down for 18 ℃ in temperature, get final product when the radicle of 80% above seed is broken through seed 1/2 above length, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 7:
Select seed clean, full, neat and consistent; Selected seed is immersed 70% alcohol disinfecting 25s, and draining and inserting concentration of volume percent to the no dropping liquid phenomenon is 15% H
2O
2Solution soaking 18min, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, seed behind the cancellation poison, 25h soaks seed in the Gibberellins solution of concentration 900mg/L, with 4 times of weight aseptic water washings 1 time, spread 1 layer of aseptic filter paper as germinating bed, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down between the seed, keeping envionmental humidity is 70%, cultivated 8 days down for 16 ℃ in temperature, duration of germination is observed the record germination, and when getting final product 2/3 or more and during the radicle of seed breakthrough seed 1/2 above length, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 8:
Select seed clean, full, neat and consistent; Selected seed is immersed 55% alcohol disinfecting 30s, and draining and inserting concentration of volume percent to the no dropping liquid phenomenon is 12% H
2O
2Solution soaking 20min, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, seed behind the cancellation poison, 20h soaks seed in the Gibberellins solution of concentration 1200mg/L, with 3 times of weight aseptic water washings 2 times, spread 3 layers of aseptic filter paper as germinating bed, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down between the seed, keeping envionmental humidity is 65%, cultivated 12 days down for 15 ℃ in temperature, duration of germination is observed the record germination, and when getting final product 2/3 or more and during the radicle of seed breakthrough seed 1/2 above length, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 9:
Seed selection: select clean, full, the seed of neat and consistent, selected seed is immersed 75% alcohol disinfecting 30s, draining and inserting weight percent concentration to the no dropping liquid phenomenon is 5% NaClO solution soaking 15min, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, seed after will sterilizing on the casting bed after fully sterilizing is tiled in the aseptic germination bed, supreme stack situation down between the seed, keeping envionmental humidity is 70%, cultivated 50 days down for 5 ℃ in temperature, duration of germination is observed the record germination, get final product when the radicle of 70% seed is broken through seed 1/2 above length, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 10:
Seed selection: select clean, full, the seed of neat and consistent, selected seed is immersed 65% alcohol disinfecting 25s, draining and inserting weight percent concentration to the no dropping liquid phenomenon is 8% NaClO solution soaking 20min, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, with casting bed behind 200 ℃ of steam sterilizing 30min, be cooled to 10 ℃, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down between the seed, keeping envionmental humidity is 55%, cultivated 40 days down for 10 ℃ in temperature, duration of germination is observed the record germination, gets final product when the radicle of 75% seed is broken through seed 1/2 above length, and gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 11:
Seed selection: select clean, full, the seed of neat and consistent, selected seed is immersed 50% alcohol disinfecting 25s, draining and inserting weight percent concentration to the no dropping liquid phenomenon is 3% NaClO solution soaking 20min, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, seed after will sterilizing on 2 layers of aseptic filter paper is tiled in the aseptic germination bed, supreme stack situation down between the seed, keeping envionmental humidity is 75%, cultivated 40 days down for 4 ℃ in temperature, duration of germination is observed the record germination, get final product when the radicle of 70% seed is broken through seed 1/2 above length, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 12:
Seed selection: select clean, full, the seed of neat and consistent, selected seed is immersed 50% alcohol disinfecting 35s, draining and inserting weight percent concentration to the no dropping liquid phenomenon is 6% NaClO solution soaking 15min, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down is advisable between the seed, keeping envionmental humidity is 55%, cultivated 50 days down for 8 ℃ in temperature, duration of germination is observed the record germination, get final product when the radicle of 80% seed is broken through seed 1/2 above length, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 13:
Seed selection: select clean, full, the seed of neat and consistent, selected seed is immersed 75% alcohol disinfecting 30s, draining and inserting weight percent concentration to the no dropping liquid phenomenon is to soak 15min in 0.8% the HgCI solution, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, seed after will sterilizing on the casting bed after fully sterilizing is tiled in the aseptic germination bed, supreme stack situation down between the seed, keeping envionmental humidity is 70%, cultivated 50 days down for 5 ℃ in temperature, duration of germination is observed the record germination, get final product when the radicle of 70% seed is broken through seed 1/2 above length, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 14:
Seed selection: select clean, full, the seed of neat and consistent, selected seed is immersed 65% alcohol disinfecting 25s, draining and inserting weight percent concentration to the no dropping liquid phenomenon is to soak 25min in 1.2% the HgCI solution, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, with casting bed behind 180 ℃ of steam sterilizing 30min, be cooled to 8 ℃, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down between the seed, keeping envionmental humidity is 70%, cultivated 55 days down for 8 ℃ in temperature, duration of germination is observed the record germination, gets final product when the radicle of 75% seed is broken through seed 1/2 above length, and gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 15:
Seed selection: select clean, full, the seed of neat and consistent, selected seed is immersed 60% alcohol disinfecting 25s, draining and inserting weight percent concentration to the no dropping liquid phenomenon is to soak 20min in 1% the HgCI solution, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, seed after will sterilizing on 1 layer of aseptic filter paper is tiled in the aseptic germination bed, supreme stack situation down between the seed, keeping envionmental humidity is 65%, cultivated 55 days down for 5 ℃ in temperature, duration of germination is observed the record germination, get final product when the radicle of 70% seed is broken through seed 1/2 above length, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 16:
Seed selection: select clean, full, the seed of neat and consistent, selected seed is immersed 70% alcohol disinfecting 35s, draining and inserting weight percent concentration to the no dropping liquid phenomenon is to soak 15min in 1.2% the HgCI solution, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down is advisable between the seed, keeping envionmental humidity is 65%, cultivated 50 days down for 6 ℃ in temperature, duration of germination is observed the record germination, get final product when the radicle of 75% seed is broken through seed 1/2 above length, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 17:
Seed selection: select clean, full, the seed of neat and consistent, selected seed is immersed 65% alcohol disinfecting 30s, draining and inserting weight percent concentration to the no dropping liquid phenomenon is to soak 25min in 1.1% the HgCI solution, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, seed after will sterilizing on 3 layers of aseptic filter paper is tiled in the aseptic germination bed, supreme stack situation down between the seed, keeping envionmental humidity is 75%, cultivated 15 days down for 15 ℃ in temperature, duration of germination is observed the record germination, get final product when the radicle of 60% seed is broken through seed 1/2 above length, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 18:
Seed selection: select clean, full, the seed of neat and consistent, selected seed is immersed 55% alcohol disinfecting 35s, draining and inserting weight percent concentration to the no dropping liquid phenomenon is to soak 20min in 1.2% the HgCI solution, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down is advisable between the seed, keeping envionmental humidity is 65%, cultivated 20 days down for 20 ℃ in temperature, duration of germination is observed the record germination, get final product when the radicle of 65% seed is broken through seed 1/2 above length, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 19:
Seed selection: select clean, full, the seed of neat and consistent, selected seed is immersed 55% alcohol disinfecting 35s, draining and inserting weight percent concentration to the no dropping liquid phenomenon is to soak 20min in 1.2% the HgCI solution, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, seed after the sterilization is tiled in 2 layers of aseptic filter paper germinating bed, supreme stack situation down is advisable between the seed, keeping envionmental humidity is 65%, cultivated 10 days down for 20 ℃ in temperature, duration of germination is observed the record germination, when breaking through seed 1/2 above length, the radicle of 65% seed gets final product, if the seed bud ratio does not reach predetermined value, can prolong 5 days again, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 20:
Seed selection: select clean, full, the seed of neat and consistent, selected seed is immersed 70% alcohol disinfecting 30s, draining and inserting weight percent concentration to the no dropping liquid phenomenon is to soak 30min in 0.9% the HgCI solution, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, seed after the sterilization is tiled in 3 layers of aseptic filter paper germinating bed, supreme stack situation down is advisable between the seed, keeping envionmental humidity is 75%, cultivated 15 days down for 15 ℃ in temperature, duration of germination is observed the record germination, when breaking through seed 1/2 above length, the radicle of 60% seed gets final product, if the seed bud ratio does not reach predetermined value, can prolong 5 days again, gained can be used as next step and carries out tissue culture, seedling plantation or move the material of sanction etc.
Embodiment 21:
Seed selection: select seed clean, full, neat and consistent, selected seed is immersed 75% alcohol disinfecting 25s, draining and inserting concentration of volume percent to the no dropping liquid phenomenon is 15% H
2O
2Solution soaking 15min, drain to the no dropping liquid phenomenon with the aseptic water washing of 2 times of weight 3 times, drain to the nothing situation of dripping, get 3 layers of tiling of aseptic filter paper as germinating bed, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down between the seed, keep envionmental humidity 60%, cultivated 70 days and observe at any time the record germination down for 5 ℃ in temperature, get final product when the radicle of 80% above seed is broken through seed 1/2 above length, gained can be used as that next step carries out tissue culture, seedling is planted or moves the material of sanction etc.
Embodiment 22:
Seed selection: select seed clean, full, neat and consistent, selected seed is immersed 55% alcohol disinfecting 35s, draining and inserting concentration of volume percent to the no dropping liquid phenomenon is 13% H
2O
2Solution soaking 12min, drain to the no dropping liquid phenomenon with the aseptic water washing of 3 times of weight 3 times, drain to the nothing situation of dripping, get 2 layers of tiling of aseptic filter paper as germinating bed, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down between the seed, keep envionmental humidity 55%, cultivated 50 days and observe at any time the record germination down for 8 ℃ in temperature, get final product when the radicle of 80% above seed is broken through seed 1/2 above length, gained can be used as that next step carries out tissue culture, seedling is planted or moves the material of sanction etc.
Embodiment 23:
Select seed clean, full, neat and consistent; Selected seed is immersed 70% alcohol disinfecting 25s, and draining and inserting concentration of volume percent to the no dropping liquid phenomenon is 15% H
2O
2Solution soaking 18min, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, spread 1 layer of aseptic filter paper as germinating bed, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down between the seed, keeping envionmental humidity is 70%, cultivated 45 days down for 10 ℃ in temperature, duration of germination is observed the record germination, when more than 2/3 and the radicle of seed get final product when breaking through seed 1/2 above length, gained can be used as that next step carries out tissue culture, seedling is planted or moves the material of sanction etc.
Embodiment 24:
Select seed clean, full, neat and consistent; Selected seed is immersed 55% alcohol disinfecting 30s, and draining and inserting concentration of volume percent to the no dropping liquid phenomenon is 12% H
2O
2Solution soaking 20min, drain to the no dropping liquid phenomenon clean with aseptic water washing, drain to the nothing situation of dripping, spread 3 layers of aseptic filter paper as germinating bed, seed after the sterilization is tiled in the aseptic germination bed, supreme stack situation down between the seed, keeping envionmental humidity is 65%, cultivated 50 days down for 6 ℃ in temperature, duration of germination is observed the record germination, when more than 2/3 and the radicle of seed get final product when breaking through seed 1/2 above length, gained can be used as that next step carries out tissue culture, seedling is planted or moves the material of sanction etc.
Claims (7)
1, a kind of method for cultivating aseptic seedling of Ferula sinkiangensis is characterized in that being achieved through the following technical solutions:
Seed selection:
Select seed clean, full, neat and consistent;
Sterilization:
After selected seed immersed 50～80% alcohol, 25～35s, insert concentration of volume percent after draining and be 10～15% H
2O
2Solution soaking 10～20min, clean with aseptic water washing, drain;
Putting bed cultivates:
Seed after the sterilization is tiled in the aseptic germination bed, and keeping envionmental humidity is 25～75%, cultivates 7～60 days down for 2～20 ℃ in temperature.
2, method for cultivating aseptic seedling of Ferula sinkiangensis according to claim 1 is characterized in that described putting in the bed cultivation, cultivates 20～60 days under 2～15 ℃ of conditions.
3, method for cultivating aseptic seedling of Ferula sinkiangensis according to claim 2 is characterized in that described temperature condition is 4～10 ℃.
4, method for cultivating aseptic seedling of Ferula sinkiangensis according to claim 1, it is characterized in that described putting in the bed cultivation, seed behind the cancellation poison, 15～30h soaks seed in the Gibberellins solution of concentration 800～1200mg/L, clean with aseptic water washing, place on the germinating bed after the sterilization, under 15～20 ℃ of conditions, cultivated 7～15 days.
5,, it is characterized in that described germinating bed is a kind of in the filter paper bed that constitutes of casting bed or at least one metafiltration paper according to claim 1,2,3 or 4 described method for cultivating aseptic seedling of Ferula sinkiangensis.
6, according to claim 1,2,3 or 4 described method for cultivating aseptic seedling of Ferula sinkiangensis, it is characterized in that the H in the described disinfectant program
2O
2Solution is that 3～8% the NaClO aqueous solution or weight percent concentration are that 0.8～1.2% HgCI solution is replaced by weight percent concentration.
7, method for cultivating aseptic seedling of Ferula sinkiangensis according to claim 5 is characterized in that the H in the described disinfectant program
2O
2Solution is that 3～8% the NaClO aqueous solution or weight percent concentration are that 0.8～1.2% HgCI solution is replaced by weight percent concentration.
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| CN105917958A (en) * | 2016-04-15 | 2016-09-07 | 新疆农业科学院微生物应用研究所 | Method for researching interaction between Pleurotus ferulae fungi and eremophyte ferula asafoetida seed germination |
| CN108338043A (en) * | 2018-02-13 | 2018-07-31 | 新疆维吾尔自治区中药民族药研究所 | A kind of method of medicinal asafoetide artificial growth cultivation |
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2009
- 2009-02-13 CN CNA2009101132242A patent/CN101480123A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104919935A (en) * | 2015-05-31 | 2015-09-23 | 贵州黔西南喀斯特区域发展研究院 | Method for promoting rapid germination of coix lacryma-jobi seeds |
| CN105917958A (en) * | 2016-04-15 | 2016-09-07 | 新疆农业科学院微生物应用研究所 | Method for researching interaction between Pleurotus ferulae fungi and eremophyte ferula asafoetida seed germination |
| CN105917958B (en) * | 2016-04-15 | 2018-11-20 | 新疆农业科学院微生物应用研究所 | A method of for studying Pleurotus ferulae fungi and ermophyte asafoetide germination interaction |
| CN108338043A (en) * | 2018-02-13 | 2018-07-31 | 新疆维吾尔自治区中药民族药研究所 | A kind of method of medicinal asafoetide artificial growth cultivation |
| CN108338043B (en) * | 2018-02-13 | 2020-04-28 | 新疆维吾尔自治区中药民族药研究所 | Method for artificially planting and cultivating medicinal asafetida |
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