CN101444229A - Kojic acid plant growth regulator and preparation process thereof - Google Patents
Kojic acid plant growth regulator and preparation process thereof Download PDFInfo
- Publication number
- CN101444229A CN101444229A CNA2008102364971A CN200810236497A CN101444229A CN 101444229 A CN101444229 A CN 101444229A CN A2008102364971 A CNA2008102364971 A CN A2008102364971A CN 200810236497 A CN200810236497 A CN 200810236497A CN 101444229 A CN101444229 A CN 101444229A
- Authority
- CN
- China
- Prior art keywords
- liquid
- fermentation
- kojic acid
- preparation
- plant growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the technical field of microbe fermentation products, and a preparation method thereof, in particular to a kojic acid plant growth regulator and the preparation process thereof. To solve the problem that the prior art is not ideal in the aspects of promoting a plant to increase production and improving quality. The invention adopts the following solution : the technique for preparing the kojic acid plant growth regulator comprises the following steps: step 1, preparing liquid fermentation medium by glucose, potassium phosphate dibasic, potassium chloride, magnesium sulphate, ferrous sulfate and ammonium nitrate, adding aspergillus by aseptic operation to ferment spawn, stirring, and fermenting continuously; step 2, heating the mature fermented liquid for carrying out solid-liquid separation to obtain a transparent liquid in yellow to tan; and step 3, decompressing and concentrating to obtain a brown liquid preparation. Compared with the prior art, the kojic acid plant growth regulator and the preparation process thereof which adopt microbe fermentation production process have the advantages that the emphytic character of the produced spawn is stable; the product has a definite formula and functional components; and obvious effects on production increasing and quality improving after vegetable spraying facilities.
Description
Technical field:
The invention belongs to microbial fermentation product and preparation method thereof technical field, be specifically related to a kind of kojic acid plant growth regulator and preparation technology thereof.
Background technology:
Kojic acid is a kind of faintly acid compound that utilizes starch sugar raw material aerobic fermentation to produce by microorganism, chemical name 2-methylol-5-hydroxyl-γ-pyrones, its structure is similar to glucose, confirm that through isotope experiment the biosynthesis pathway of kojic acid is to be formed by glucose direct oxidation dehydration.Chemical composition and molecular structure according to kojic acid, that kojic acid has is antibacterial, anti-oxidant, suppress character such as polyphenol oxidase, chelated metal ions, can be used as the antibiotic antiseptic of food, fruits and vegetables prevent the antistaling agent of enzymatic browning, the antioxidation and color-protecting agent of meat products, aquatic products, the brightening, dispelling spots, dispelling function base-material of skin-lightening cosmetic.Therefore, can see that kojic acid has application promise in clinical practice in industries such as food processing, garden stuff processing, medical material production, cosmetics, be the particularly comparatively active a kind of organic acid of product application and development of Recent study, and its application constantly obtains exploitation.The organic acid plant growth regulator is widely used in agricultural production, but prior art is promoting crop yield and improving aspect the quality not ideal enough.
Summary of the invention:
The invention provides a kind of kojic acid plant growth regulator and preparation technology thereof, promoting crop yield and improving unfavorable problem aspect the quality to solve prior art.
For solving the problem that prior art exists, technical solution of the present invention is:
A kind of kojic acid plant growth regulator is prepared from by following method:
Step 1, fermentation: the part by weight by glucose 10～12%, dipotassium hydrogen phosphate 0.05～0.1%, potassium chloride 0.025～0.05%, magnesium sulfate 0.025～0.05%, ferrous sulfate 0.001～0.002%, yeast extract 0.1～0.2%, ammonium nitrate 0.2～0.4% prepares liquid fermentation medium, routine operation is to the fermentation medium sterilization treatment, 6～10% sterile workings on weight of solution insert the aspergillus fermented bacterium, 30 ± 1 ℃ of steady temperatures, mixing speed 150～200rpm/min, ventilation 0.27～0.3M
3/ M
3Min, tank pressure 0.065～0.07MPa, continuing fermentation stop fermentation when measuring zymotic fluid kojic acid content and reach 3～5g/100ml;
Step 2 is extracted and is separated: maturing fermentation liquid is heated to 80～85 ℃, kept 15～20 minutes, carry out Separation of Solid and Liquid, obtain yellow to brown yellow transparent liquid;
Step 3, preparation concentrates: filtrate is at 60～70 ℃, and vacuum 13～14KPa is evaporated to 50% volume, obtains the brown liquid preparation.
A kind of preparation method of kojic acid plant growth regulator comprises the steps successively,
Step 1, fermentation: the part by weight by glucose 10～12%, dipotassium hydrogen phosphate 0.05～0.1%, potassium chloride 0.025～0.05%, magnesium sulfate 0.025～0.05%, ferrous sulfate 0.001～0.002%, yeast extract 0.1～0.2%, ammonium nitrate 0.2～0.4% prepares liquid fermentation medium, routine operation is to the fermentation medium sterilization treatment, 6～10% sterile workings on weight of solution insert the aspergillus fermented bacterium, 30 ± 1 ℃ of steady temperatures, mixing speed 150～200rpm/min, ventilation 0.27～0.3M
3/ M
3Min, tank pressure 0.065～0.07MPa, continuing fermentation stop fermentation when measuring zymotic fluid kojic acid content and reach 3～5g/100ml;
Step 2 is extracted and is separated: maturing fermentation liquid is heated to 80～85 ℃, kept 15～20 minutes, carry out Separation of Solid and Liquid, obtain yellow to brown yellow transparent liquid;
Step 3, preparation concentrates: filtrate is at 60～70 ℃, and vacuum 13～14KPa is evaporated to 50% volume, obtains the brown liquid preparation.
The present invention's advantage compared with prior art is:
The present invention is raw material with glucose, auxilliary dipotassium hydrogen phosphate, potassium chloride, magnesium sulfate, ferrous sulfate, yeast extract and the ammonium nitrate of joining, be mixed with the nutrient medium that contains various ingredients, be converted into the kojic acid plant growth regulator through the micro-organisms aspergillus strain fermentation, this kojic acid plant growth regulator can promote crop yield, improve quality.The present invention adopts the microbial fermentation production technology, has definite technological process and process operation parameter, produces the bacterial classification hereditary capacity and stablizes, and product is made up of and functional component clear and definite prescription, after facilities vegetable sprays, tangible volume increase and the effect that improves quality is arranged.
Embodiment:
To explain the present invention by specific embodiment below.
Embodiment 1:
A kind of kojic acid plant growth regulator is prepared from by following method:
Step 1: press the total ingredients by weight obtaining liq of 10kg fermentation medium, glucose 1.2kg wherein, dipotassium hydrogen phosphate 0.01kg, potassium chloride 0.005kg, magnesium sulfate 0.005kg, ferrous sulfate 0.0002kg, yeast extract 0.02kg, ammonium nitrate 0.04kg, surplus is a water.Regulating pH with sulfuric acid is 5.0, heat temperature raising to 120 ℃, constant 30 minutes, routine operation sterilization treats that the medium temperature reduces to 30 ℃, inserts 1kg kojic acid fermented liquid bacterial classification, 30 ± 1 ℃ of steady temperatures, mixing speed is at 1～72hr control 150rpm/min, and at 73～120hr control 200rpm/min, ventilation is at 1～72hr control 0.27M
3/ M
3Min is at 73～120hr control 0.3M
3/ M
3Min, tank pressure are at 1～72hr control 0.065MPa, and at 73～120hr control 0.07MPa, continuing fermentation 120hr begins to measure zymotic fluid kojic acid content, promptly stop fermentation when reaching 5g/100ml.
Step 2, extract and separate: maturing fermentation liquid is heated to 80～85 ℃, keeps 15 minutes, obtains pale brown look filtrate with the press filtration mode,
Step 3, preparation concentrates: use the concentrating under reduced pressure device, under the condition of 60～70 ℃ of temperature, vacuum 13KPa, concentrating under reduced pressure obtains the 5.0kg preparation.
Embodiment 2:
A kind of kojic acid plant growth regulator is prepared from by following method:
Step 1: press the total ingredients by weight obtaining liq of 50kg fermentation medium, glucose 5.0kg wherein, dipotassium hydrogen phosphate 0.025kg, potassium chloride 0.0125kg, magnesium sulfate 0.0125kg, ferrous sulfate 0.0005kg, yeast extract 0.05kg, ammonium nitrate 0.1kg, surplus is a water.Regulating pH with sulfuric acid is 5.0, heat temperature raising to 120 ℃, constant 30 minutes, routine operation sterilization treats that the medium temperature reduces to 30 ℃, inserts 4kg kojic acid fermented liquid bacterial classification, 30 ± 1 ℃ of steady temperatures, mixing speed is at 1～72hr control 150rpm/min, and at 73～120hr control 200rpm/min, ventilation is at 1～72hr control 0.27M
3/ M
3Min is at 73～120hr control 0.3M
3/ M
3Min, tank pressure are at 1-72hr control 0.065MPa, and at 73-120hr control 0.07MPa, continuing fermentation 120hr begins to measure zymotic fluid kojic acid content, promptly stop fermentation when reaching 4g/100ml.
Step 2, extract and separate: maturing fermentation liquid is heated to 80～85 ℃, keeps 20 minutes, obtains pale brown look filtrate with the press filtration mode;
Step 3, preparation concentrates: use the concentrating under reduced pressure device, under the condition of 60～70 ℃ of temperature, vacuum 14KPa, concentrating under reduced pressure obtains the 25kg preparation.
Embodiment 3:
A kind of kojic acid plant growth regulator is prepared from by following method:
Step 1: press the total ingredients by weight obtaining liq of 10kg fermentation medium, glucose 1.1kg wherein, dipotassium hydrogen phosphate 0.01kg, potassium chloride 0.0025kg, magnesium sulfate 0.005kg, ferrous sulfate 0.0002kg, yeast extract 0.01kg, ammonium nitrate 0.04kg, surplus is a water.Regulating pH with sulfuric acid is 5.0, heat temperature raising to 140 ℃, constant 25 minutes, routine operation sterilization treats that the medium temperature reduces to 30 ℃, inserts 0.8kg kojic acid fermented liquid bacterial classification, 30 ± 1 ℃ of steady temperatures, mixing speed is at 1～72hr control 150rpm/min, and at 73～120hr control 200rpm/min, ventilation is at 1～72hr control 0.27M
3/ M
3Min is at 73～120hr control 0.3M
3/ M
3Min, tank pressure are at 1-72hr control 0.065MPa, and at 73-120hr control 0.07MPa, continuing fermentation 120hr begins to measure zymotic fluid kojic acid content, promptly stop fermentation when reaching 5g/100ml.
Step 2, extract and separate: maturing fermentation liquid is heated to 80～85 ℃, keeps 20 minutes, obtains pale brown look filtrate with the press filtration mode,
Step 3, preparation concentrates: use the concentrating under reduced pressure device, under the condition of 60～70 ℃ of temperature, vacuum 13KPa, concentrating under reduced pressure obtains the 5.0kg preparation.
Using method: with the said goods through water dilution back immersion seed or be sprayed onto vegetable surface and use as plant growth regulator.As 10 times of dilute with waters, soaked wheat, corn, soybean, cucumber seed kind 30 minutes, can effectively improve the germination rate of above-mentioned seed kind, increase plant height, root length, bright root heavily reach bright cauline leaf and weigh, and as with behind 0.75%, 0.375% the kojic acid plant growth regulator seed soaking cucumber, grow 20 days, average plant height is respectively 16.8cm and 16.6cm, that clear water contrasts is 6.3cm, and root length is respectively 5.7cm and 5.3cm, and contrasts only 4.77cm.As 15 times of dilute with waters, spray greenhouse cucumber, can shorten seedling-slowing stage, promote the cucumber seedling growing way, increase plant height, the number of blade, as with 0.5%, 0.25% kojic acid plant growth regulator to the cucumber plant foliar spray, help upright slow seedling, the shortening seedling-slowing stage of planting of cucumber.After using 3 times continuously, the cucumber seedling growing way of 0.5% kojic acid preparation treatment region is best, and plant height contrast increases 13.5-25cm, and the number of blade increases the 1-3 sheet, and the leaf look dark green, and growth potential is strong.This product has the promotion growth and development of plants, improves the effect of quality, can and be applied to agricultural production as seed soaking agent, the foliage-spray of crops.
Claims (2)
1, a kind of kojic acid plant growth regulator is prepared from by following method:
Step 1, fermentation: the part by weight by glucose 10～12%, dipotassium hydrogen phosphate 0.05～0.1%, potassium chloride 0.025～0.05%, magnesium sulfate 0.025～0.05%, ferrous sulfate 0.001～0.002%, yeast extract 0.1～0.2%, ammonium nitrate 0.2～0.4% prepares liquid fermentation medium, routine operation is to the fermentation medium sterilization treatment, 6～10% sterile workings on weight of solution insert the aspergillus fermented bacterium, 30 ± 1 ℃ of steady temperatures, mixing speed 150～200rpm/min, ventilation 0.27～0.3M
3/ M
3Min, tank pressure 0.065～0.07MPa, continuing fermentation stop fermentation when measuring zymotic fluid kojic acid content and reach 3～5g/100ml;
Step 2 is extracted and is separated: maturing fermentation liquid is heated to 80～85 ℃, kept 15～20 minutes, carry out Separation of Solid and Liquid, obtain yellow to brown yellow transparent liquid;
Step 3, preparation concentrates: filtrate is at 60～70 ℃, and vacuum 13～14KPa is evaporated to 50% volume, obtains the brown liquid preparation.
2, the preparation method of a kind of kojic acid plant growth regulator as claimed in claim 1 comprises the steps successively,
Step 1, fermentation: the part by weight by glucose 10～12%, dipotassium hydrogen phosphate 0.05～0.1%, potassium chloride 0.025～0.05%, magnesium sulfate 0.025～0.05%, ferrous sulfate 0.001～0.002%, yeast extract 0.1～0.2%, ammonium nitrate 0.2～0.4% prepares liquid fermentation medium, routine operation is to the fermentation medium sterilization treatment, 6～10% sterile workings on weight of solution insert the aspergillus fermented bacterium, 30 ± 1 ℃ of steady temperatures, mixing speed 150～200rpm/min, ventilation 0.27～0.3M
3/ M
3Min, tank pressure 0.065～0.07MPa, continuing fermentation stop fermentation when measuring zymotic fluid kojic acid content and reach 3～5g/100ml;
Step 2 is extracted and is separated: maturing fermentation liquid is heated to 80～85 ℃, kept 15～20 minutes, carry out Separation of Solid and Liquid, obtain yellow to brown yellow transparent liquid;
Step 3, preparation concentrates: filtrate is at 60～70 ℃, and vacuum 13～14KPa is evaporated to 50% volume, obtains the brown liquid preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810236497 CN101444229B (en) | 2008-12-29 | 2008-12-29 | Kojic acid plant growth regulator and preparation process thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810236497 CN101444229B (en) | 2008-12-29 | 2008-12-29 | Kojic acid plant growth regulator and preparation process thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101444229A true CN101444229A (en) | 2009-06-03 |
| CN101444229B CN101444229B (en) | 2013-05-22 |
Family
ID=40740342
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200810236497 Active CN101444229B (en) | 2008-12-29 | 2008-12-29 | Kojic acid plant growth regulator and preparation process thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101444229B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321700A (en) * | 2011-08-03 | 2012-01-18 | 无锡赛德生物工程有限公司 | Kojic acid fermentation technique and apparatus |
| CN107006167A (en) * | 2017-03-17 | 2017-08-04 | 界首市兴邦家庭农场 | A kind of method for culturing seedlings of soya seeds bud rate occurred frequently |
| CN113481016A (en) * | 2021-07-29 | 2021-10-08 | 海南金象云农业科技有限公司 | Soil conditioner and preparation method and application thereof |
-
2008
- 2008-12-29 CN CN 200810236497 patent/CN101444229B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 王博彦, 等: "《发酵有机酸生产与应用手册》", 30 September 2000, 中国轻工业出版社 * |
| 陆正清，等: "曲酸的发酵法生产及其在食品加工中的应用", 《中国调味品》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321700A (en) * | 2011-08-03 | 2012-01-18 | 无锡赛德生物工程有限公司 | Kojic acid fermentation technique and apparatus |
| CN107006167A (en) * | 2017-03-17 | 2017-08-04 | 界首市兴邦家庭农场 | A kind of method for culturing seedlings of soya seeds bud rate occurred frequently |
| CN113481016A (en) * | 2021-07-29 | 2021-10-08 | 海南金象云农业科技有限公司 | Soil conditioner and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101444229B (en) | 2013-05-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102010240B (en) | Plant enzyme foliar fertilizer and preparation method thereof | |
| CN106190699B (en) | A kind of preparation method of brown rice wine of rice fermented with red yeast | |
| KR101799780B1 (en) | Method for Manufacturing Plant Nutrient Containing Mineral Element | |
| CN101270329B (en) | Method for preparing high concentration fruit vinegar with liquid state submerged fermentation | |
| CN104350954B (en) | Cornu Cervi Pantotrichum mushroom cultivation method | |
| CN105036921A (en) | Plant nutrient solution and preparing method thereof | |
| CN107759262A (en) | A kind of fertilizing method of high yield high nutrition quality red bayberry | |
| CN107996164A (en) | A kind of selenium enriched tea cuttage and seedling culture method | |
| CN108040792A (en) | A kind of implantation methods of good quality and high output peanut | |
| CN100535104C (en) | Method for preparing feedstuff yeast from maize peel hydrolysis solution | |
| CN106665044A (en) | Cultivation method of selenium-enriched tomato | |
| CN108476863A (en) | A kind of cultivation base stock and preparation method thereof of sliding mushroom | |
| CN103449900B (en) | Special liquid organic fertilizer for tea and preparation method of liquid organic fertilizer | |
| CN102381896B (en) | Crab-flavor mushroom liquid strain medium formula and preparation method thereof | |
| CN103725626B (en) | Bacillus laterosporus preparation | |
| CN101444229B (en) | Kojic acid plant growth regulator and preparation process thereof | |
| CN109836298A (en) | A kind of biological humic acid ferment liquid fertilizer and the preparation method and application thereof | |
| CN109618827A (en) | A kind of cynomorium songaricum high-yield disease resisting cultural method | |
| CN107517714A (en) | A kind of cultural method of high yield high nutrition strawberry | |
| CN108029308A (en) | A kind of fertilizing method for lifting pumpkin mouthfeel | |
| CN106995347A (en) | A kind of Flower Plants in Hydroponic Culture nutrient solution and preparation method thereof | |
| CN106518193A (en) | Zinc-rich organic fertilizer | |
| CN110117205B (en) | Germanium-rich nutrient solution for increasing germanium content of rapeseeds and preparation method and application thereof | |
| CN103387427B (en) | Method for manufacture of biofertilizer by pleuromutilin bacterial residue harmless treatment | |
| CN107509522A (en) | A kind of cultural method of high yield high-quality watermelon |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |