CN107006167A - A kind of method for culturing seedlings of soya seeds bud rate occurred frequently - Google Patents
A kind of method for culturing seedlings of soya seeds bud rate occurred frequently Download PDFInfo
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- CN107006167A CN107006167A CN201710159763.4A CN201710159763A CN107006167A CN 107006167 A CN107006167 A CN 107006167A CN 201710159763 A CN201710159763 A CN 201710159763A CN 107006167 A CN107006167 A CN 107006167A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
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- Natural Medicines & Medicinal Plants (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
A kind of disclosure of the invention method for culturing seedlings of soya seeds bud rates occurred frequently：The preparation of soya seeds；The dipping pretreatment of soya seeds；It is prepared by soya seeds treatment fluid；Soya seeds processing；Soya seeds vernalization；Soya seeds are sowed.Compared with existing soybean seedling-raising technique, the present invention has extremely significant advantage, and the soya seeds germination percentage that the present invention is handled is up to more than 99.2%, and seedling replanting survival rate is 96.5%.
Description
Technical field
The present invention relates to field of planting, the method for culturing seedlings of more particularly to a kind of soya seeds bud rate occurred frequently.
Background technology
Soybean, is generally called soya bean, originates in China, has cultivation all over China, also cultivate extensively in all over the world, during soybean is
One of important cereal crops of state, existing 5,000 years cultivation histories.Soybean nursery is that soybean obtains precocity, high yield, fine quality production
Important means.The selection of nursery date of seeding, the pretreatment of seed, vernalization all have vital influence to the quality of seedling.
The content of the invention
Above-mentioned in order to overcome the shortcomings of, the invention provides a kind of method for culturing seedlings of soya seeds bud rate occurred frequently.
The present invention is achieved through the following technical solutions：
A kind of method for culturing seedlings of soya seeds bud rate occurred frequently, comprises the following steps：
The preparation of a soya seeds：Natural maturity, healthy and strong soya seeds is selected to be used for nursery；
The dipping pretreatment of b soya seeds：Soya seeds are put into the bay acid solution that mass percent at 32-36 DEG C is 0.1%
After middle immersion 1.5 hours, take out, drain；
It is prepared by c soya seeds treatment fluid：Navy bean is mixed with water, is beaten, is incubated 20min at 38 DEG C, forming water content is
Aspergillus flora, is then inoculated in the mixture of above-mentioned gained, obtains culture by the mixture of 40% percentage by weight, its
In, the aspergillus flora is：Aspergillus oryzae, aspergillus niger, monascus three's mixed in equal amounts, and the inoculum concentration of the aspergillus flora accounts for navy bean
The 0.014% of weight；Above-mentioned gained culture progress solid state fermentation is obtained into tunning, wherein, the condition of the solid state fermentation
Including：Relative humidity 80%, fermentation temperature are 32 DEG C, and the solid state fermentation time is 25 hours, and above-mentioned tunning is hydrolyzed
The mixed weight ratio that reaction obtains tunning and water in hydrolyzate, hydrolysis is 1：1.2, hydrolysis condition is：Temperature
It is 18 hours for 42 DEG C, time, above-mentioned hydrolyzate is centrifuged into 30min under 2500r/min rotating speeds, then stands 2 hours, mistake
Filter, retains supernatant liquid, carries out sterilization treatment, then adds the sodium carbonate of clarified solution quality 0.5%, after stirring, produces
Required soya seeds treatment fluid；
The processing of d soya seeds：Soya seeds and soya seeds treatment fluid are pressed 1：50-55 mass ratios uniformly after mixing, are heated
To 30 DEG C, 1 hour is incubated, then by temperature adjustment to 28 DEG C, is stirred 2.5 hours with 30r/min rotating speeds, then stops stirring,
At 28 DEG C, using 38kHz, 300W ultrasonication 1.5min, soya seeds are taken out, are drained naturally at 28 DEG C；
E soya seeds vernalization：Soya seeds are placed in vernalization 20 hours under 28-35 DEG C of environment temperature, treat that 80% soya seeds reveal
It is white；
F soya seeds are sowed：The soya seeds showed money or valuables one carries unintentionally are sowed, soya seeds surface earthing 0.5cm, at soya seeds
Manage liquid and press 1 with water：It is prepared into after the mixing of 50-60 mass ratios and pours liquid filling, liquid filling will be poured the soybean just sowed is poured, table
Face is uniformly poured, and is 1kg per square meter irrigation amount, after 2 days, then is poured once, irrigation amount is identical with first time.
Immersion treatment of the soya seeds treatment fluid prepared by using the present invention to soya seeds at different temperatures, energy
Enough significantly improve the proteinase in soya seeds, promote to decompose the protein stored in endosperm, fat there is provided
Energy and material required for soybean germination, so as to promote the sprouting speed of soya seeds, by using Soybean Species after broadcasting
Subprocessing liquid, which is watered, to be poured, and soybean seedling root system can be promoted to develop, and promotes suction of the soybean seedling root system to nutriment
Receive, simultaneously, additionally it is possible to improve nitrate reductase activity to a certain extent, and the activity height of nitrate reductase is directly influenced
Soybean is to the assimilative capacicy of inorganic nitrogen, by the raising for improving nitrate reductase activity so that soybean seedling nitrogen assimilation ability by
It is cumulative strong, so as to promote growing for soybean seedling.
The beneficial effects of the invention are as follows：Compared with existing soybean seedling-raising technique, the present invention has extremely significant advantage, this hair
The soya seeds germination percentage of daylight reason is up to more than 99.2%, and seedling replanting survival rate is in 96.5%, the seedling cultivated, growth potential
By force, well developed root system, disease resistance is strong, and soybean yields is compared to adding for being handled without soya seeds treatment fluid of the present invention
More than 32.5%.
Embodiment
Embodiment 1
A kind of method for culturing seedlings of soya seeds bud rate occurred frequently, comprises the following steps：
The preparation of a soya seeds：Natural maturity, healthy and strong soya seeds is selected to be used for nursery；
The dipping pretreatment of b soya seeds：Soya seeds are put into mass percent at 32 DEG C in 0.1% bay acid solution
After immersion 1.5 hours, take out, drain；
It is prepared by c soya seeds treatment fluid：Navy bean is mixed with water, is beaten, is incubated 20min at 38 DEG C, forming water content is
Aspergillus flora, is then inoculated in the mixture of above-mentioned gained, obtains culture by the mixture of 40% percentage by weight, its
In, the aspergillus flora is：Aspergillus oryzae, aspergillus niger, monascus three's mixed in equal amounts, and the inoculum concentration of the aspergillus flora accounts for navy bean
The 0.014% of weight；Above-mentioned gained culture progress solid state fermentation is obtained into tunning, wherein, the condition of the solid state fermentation
Including：Relative humidity 80%, fermentation temperature are 32 DEG C, and the solid state fermentation time is 25 hours, and above-mentioned tunning is hydrolyzed
The mixed weight ratio that reaction obtains tunning and water in hydrolyzate, hydrolysis is 1：1.2, hydrolysis condition is：Temperature
It is 18 hours for 42 DEG C, time, above-mentioned hydrolyzate is centrifuged into 30min under 2500r/min rotating speeds, then stands 2 hours, mistake
Filter, retains supernatant liquid, carries out sterilization treatment, then adds the sodium carbonate of clarified solution quality 0.5%, after stirring, produces
Required soya seeds treatment fluid；
The processing of d soya seeds：Soya seeds and soya seeds treatment fluid are pressed 1：50 mass ratios uniformly after mixing, are heated to 30
DEG C, 1 hour is incubated, then by temperature adjustment to 28 DEG C, is stirred 2.5 hours with 30r/min rotating speeds, then stops stirring, 28
At DEG C, using 38kHz, 300W ultrasonication 1.5min, soya seeds are taken out, are drained naturally at 28 DEG C；
E soya seeds vernalization：Soya seeds are placed in vernalization 20 hours under 28-35 DEG C of environment temperature, treat that 80% soya seeds reveal
It is white；
F soya seeds are sowed：The soya seeds showed money or valuables one carries unintentionally are sowed, soya seeds surface earthing 0.5cm, at soya seeds
Manage liquid and press 1 with water：It is prepared into after the mixing of 50 mass ratios and pours liquid filling, liquid filling will be poured the soybean just sowed is poured, surface
It is uniform to pour, it is 1kg per square meter irrigation amount, after 2 days, then pours once, irrigation amount is identical with first time.
Embodiment 2
A kind of method for culturing seedlings of soya seeds bud rate occurred frequently, comprises the following steps：
The preparation of a soya seeds：Natural maturity, healthy and strong soya seeds is selected to be used for nursery；
The dipping pretreatment of b soya seeds：Soya seeds are put into mass percent at 36 DEG C in 0.1% bay acid solution
After immersion 1.5 hours, take out, drain；
It is prepared by c soya seeds treatment fluid：Navy bean is mixed with water, is beaten, is incubated 20min at 38 DEG C, forming water content is
Aspergillus flora, is then inoculated in the mixture of above-mentioned gained, obtains culture by the mixture of 40% percentage by weight, its
In, the aspergillus flora is：Aspergillus oryzae, aspergillus niger, monascus three's mixed in equal amounts, and the inoculum concentration of the aspergillus flora accounts for navy bean
The 0.014% of weight；Above-mentioned gained culture progress solid state fermentation is obtained into tunning, wherein, the condition of the solid state fermentation
Including：Relative humidity 80%, fermentation temperature are 32 DEG C, and the solid state fermentation time is 25 hours, and above-mentioned tunning is hydrolyzed
The mixed weight ratio that reaction obtains tunning and water in hydrolyzate, hydrolysis is 1：1.2, hydrolysis condition is：Temperature
It is 18 hours for 42 DEG C, time, above-mentioned hydrolyzate is centrifuged into 30min under 2500r/min rotating speeds, then stands 2 hours, mistake
Filter, retains supernatant liquid, carries out sterilization treatment, then adds the sodium carbonate of clarified solution quality 0.5%, after stirring, produces
Required soya seeds treatment fluid；
The processing of d soya seeds：Soya seeds and soya seeds treatment fluid are pressed 1：55 mass ratios uniformly after mixing, are heated to 30
DEG C, 1 hour is incubated, then by temperature adjustment to 28 DEG C, is stirred 2.5 hours with 30r/min rotating speeds, then stops stirring, 28
At DEG C, using 38kHz, 300W ultrasonication 1.5min, soya seeds are taken out, are drained naturally at 28 DEG C；
E soya seeds vernalization：Soya seeds are placed in vernalization 20 hours under 28-35 DEG C of environment temperature, treat that 80% soya seeds reveal
It is white；
F soya seeds are sowed：The soya seeds showed money or valuables one carries unintentionally are sowed, soya seeds surface earthing 0.5cm, at soya seeds
Manage liquid and press 1 with water：It is prepared into after the mixing of 60 mass ratios and pours liquid filling, liquid filling will be poured the soybean just sowed is poured, surface
It is uniform to pour, it is 1kg per square meter irrigation amount, after 2 days, then pours once, irrigation amount is identical with first time.
Embodiment 3
A kind of method for culturing seedlings of soya seeds bud rate occurred frequently, comprises the following steps：
The preparation of a soya seeds：Natural maturity, healthy and strong soya seeds is selected to be used for nursery；
The dipping pretreatment of b soya seeds：Soya seeds are put into mass percent at 34 DEG C in 0.1% bay acid solution
After immersion 1.5 hours, take out, drain；
It is prepared by c soya seeds treatment fluid：Navy bean is mixed with water, is beaten, is incubated 20min at 38 DEG C, forming water content is
Aspergillus flora, is then inoculated in the mixture of above-mentioned gained, obtains culture by the mixture of 40% percentage by weight, its
In, the aspergillus flora is：Aspergillus oryzae, aspergillus niger, monascus three's mixed in equal amounts, and the inoculum concentration of the aspergillus flora accounts for navy bean
The 0.014% of weight；Above-mentioned gained culture progress solid state fermentation is obtained into tunning, wherein, the condition of the solid state fermentation
Including：Relative humidity 80%, fermentation temperature are 32 DEG C, and the solid state fermentation time is 25 hours, and above-mentioned tunning is hydrolyzed
The mixed weight ratio that reaction obtains tunning and water in hydrolyzate, hydrolysis is 1：1.2, hydrolysis condition is：Temperature
It is 18 hours for 42 DEG C, time, above-mentioned hydrolyzate is centrifuged into 30min under 2500r/min rotating speeds, then stands 2 hours, mistake
Filter, retains supernatant liquid, carries out sterilization treatment, then adds the sodium carbonate of clarified solution quality 0.5%, after stirring, produces
Required soya seeds treatment fluid；
The processing of d soya seeds：Soya seeds and soya seeds treatment fluid are pressed 1：52 mass ratios uniformly after mixing, are heated to 30
DEG C, 1 hour is incubated, then by temperature adjustment to 28 DEG C, is stirred 2.5 hours with 30r/min rotating speeds, then stops stirring, 28
At DEG C, using 38kHz, 300W ultrasonication 1.5min, soya seeds are taken out, are drained naturally at 28 DEG C；
E soya seeds vernalization：Soya seeds are placed in vernalization 20 hours under 28-35 DEG C of environment temperature, treat that 80% soya seeds reveal
It is white；
F soya seeds are sowed：The soya seeds showed money or valuables one carries unintentionally are sowed, soya seeds surface earthing 0.5cm, at soya seeds
Manage liquid and press 1 with water：It is prepared into after the mixing of 55 mass ratios and pours liquid filling, liquid filling will be poured the soybean just sowed is poured, surface
It is uniform to pour, it is 1kg per square meter irrigation amount, after 2 days, then pours once, irrigation amount is identical with first time.
Comparative example 1：Soya seeds are differed only in embodiment 1 after planting to pour twice using clear water, twice irrigation amount
It is in the same manner as in Example 1.
Experiment：Soya seeds are uniformly divided into 5 groups, respectively by embodiment 1, embodiment 2, embodiment 3（Embodiment during statistics
1st, 2,3 average is taken）, comparative example 1, control group method handled, broadcast rear 48h and rise, sampling once, determines seed breathing per 24h
Speed and lipase active, are sampled 3 times altogether：
Control group：Distinguish and only handle clear water is replaced into soya seeds treatment fluid with the processing of, step d soya seeds with embodiment 1,
Step f is poured twice using clear water, and each irrigation amount is same as Example 1；
Table 1
As can be seen from Table 1, the inventive method can significantly improve soya cells respiratory rate, improve the work of intracellular lipase
Property, so as to promote seed to sprout, improve percentage of seedgermination；
Survey root activity and nitrate reductase within after planting 10 days（NR）Activity：
Table 2
The inventive method can significantly improve root activity and seedling nitrogen assimilation ability as can be seen from Table 2.
Claims (1)
1. a kind of method for culturing seedlings of soya seeds bud rate occurred frequently, it is characterised in that comprise the following steps：
The preparation of a soya seeds：Natural maturity, healthy and strong soya seeds is selected to be used for nursery；
The dipping pretreatment of b soya seeds：Soya seeds are put into the bay acid solution that mass percent at 32-36 DEG C is 0.1%
After middle immersion 1.5 hours, take out, drain；
It is prepared by c soya seeds treatment fluid：Navy bean is mixed with water, is beaten, is incubated 20min at 38 DEG C, forming water content is
Aspergillus flora, is then inoculated in the mixture of above-mentioned gained, obtains culture by the mixture of 40% percentage by weight, its
In, the aspergillus flora is：Aspergillus oryzae, aspergillus niger, monascus three's mixed in equal amounts, and the inoculum concentration of the aspergillus flora accounts for navy bean
The 0.014% of weight；Above-mentioned gained culture progress solid state fermentation is obtained into tunning, wherein, the condition of the solid state fermentation
Including：Relative humidity 80%, fermentation temperature are 32 DEG C, and the solid state fermentation time is 25 hours, and above-mentioned tunning is hydrolyzed
The mixed weight ratio that reaction obtains tunning and water in hydrolyzate, hydrolysis is 1：1.2, hydrolysis condition is：Temperature
It is 18 hours for 42 DEG C, time, above-mentioned hydrolyzate is centrifuged into 30min under 2500r/min rotating speeds, then stands 2 hours, mistake
Filter, retains supernatant liquid, carries out sterilization treatment, then adds the sodium carbonate of clarified solution quality 0.5%, after stirring, produces
Required soya seeds treatment fluid；
The processing of d soya seeds：Soya seeds and soya seeds treatment fluid are pressed 1：50-55 mass ratios uniformly after mixing, are heated
To 30 DEG C, 1 hour is incubated, then by temperature adjustment to 28 DEG C, is stirred 2.5 hours with 30r/min rotating speeds, then stops stirring,
At 28 DEG C, using 38kHz, 300W ultrasonication 1.5min, soya seeds are taken out, are drained naturally at 28 DEG C；
E soya seeds vernalization：Soya seeds are placed in vernalization 20 hours under 28-35 DEG C of environment temperature, treat that 80% soya seeds reveal
It is white；
F soya seeds are sowed：The soya seeds showed money or valuables one carries unintentionally are sowed, soya seeds surface earthing 0.5cm, at soya seeds
Manage liquid and press 1 with water：It is prepared into after the mixing of 50-60 mass ratios and pours liquid filling, liquid filling will be poured the soybean just sowed is poured, table
Face is uniformly poured, and is 1kg per square meter irrigation amount, after 2 days, then is poured once, irrigation amount is identical with first time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710159763.4A CN107006167A (en) | 2017-03-17 | 2017-03-17 | A kind of method for culturing seedlings of soya seeds bud rate occurred frequently |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710159763.4A CN107006167A (en) | 2017-03-17 | 2017-03-17 | A kind of method for culturing seedlings of soya seeds bud rate occurred frequently |
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| CN201710159763.4A Pending CN107006167A (en) | 2017-03-17 | 2017-03-17 | A kind of method for culturing seedlings of soya seeds bud rate occurred frequently |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108738484A (en) * | 2018-06-25 | 2018-11-06 | 安徽农业大学 | A kind of Kiwi berry seed initiator and cause method |
| CN108781596A (en) * | 2018-07-03 | 2018-11-13 | 全椒县马塘湖农作物种植专业合作社 | A kind of method for treating seeds improving soybean nutritional value |
| CN113455136A (en) * | 2021-08-17 | 2021-10-01 | 宁安市宏欣种业有限公司 | Soybean breeding and germination accelerating method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1101814A (en) * | 1993-10-18 | 1995-04-26 | 刘文炳 | Preparation and process for "Yicijing" peeling agent for vegetables and fruit |
| CN101444229A (en) * | 2008-12-29 | 2009-06-03 | 陕西省微生物研究所 | Kojic acid plant growth regulator and preparation process thereof |
| CN103283961A (en) * | 2013-05-10 | 2013-09-11 | 浙江工业大学 | Method for preparing health-benefiting fermented soybean meal |
| CN106258080A (en) * | 2016-08-23 | 2017-01-04 | 南阳师范学院 | A kind of method utilizing biotechnology accelerating germination Ramulus et folium taxi cuspidatae seed |
-
2017
- 2017-03-17 CN CN201710159763.4A patent/CN107006167A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1101814A (en) * | 1993-10-18 | 1995-04-26 | 刘文炳 | Preparation and process for "Yicijing" peeling agent for vegetables and fruit |
| CN101444229A (en) * | 2008-12-29 | 2009-06-03 | 陕西省微生物研究所 | Kojic acid plant growth regulator and preparation process thereof |
| CN103283961A (en) * | 2013-05-10 | 2013-09-11 | 浙江工业大学 | Method for preparing health-benefiting fermented soybean meal |
| CN106258080A (en) * | 2016-08-23 | 2017-01-04 | 南阳师范学院 | A kind of method utilizing biotechnology accelerating germination Ramulus et folium taxi cuspidatae seed |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108738484A (en) * | 2018-06-25 | 2018-11-06 | 安徽农业大学 | A kind of Kiwi berry seed initiator and cause method |
| CN108738484B (en) * | 2018-06-25 | 2021-05-25 | 安徽农业大学 | Kiwi seed initiator and initiation method |
| CN108781596A (en) * | 2018-07-03 | 2018-11-13 | 全椒县马塘湖农作物种植专业合作社 | A kind of method for treating seeds improving soybean nutritional value |
| CN113455136A (en) * | 2021-08-17 | 2021-10-01 | 宁安市宏欣种业有限公司 | Soybean breeding and germination accelerating method |
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