CN101444229B - Kojic acid plant growth regulator and preparation process thereof - Google Patents
Kojic acid plant growth regulator and preparation process thereof Download PDFInfo
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- CN101444229B CN101444229B CN 200810236497 CN200810236497A CN101444229B CN 101444229 B CN101444229 B CN 101444229B CN 200810236497 CN200810236497 CN 200810236497 CN 200810236497 A CN200810236497 A CN 200810236497A CN 101444229 B CN101444229 B CN 101444229B
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Abstract
The invention belongs to the technical field of microbe fermentation products, and a preparation method thereof, in particular to a kojic acid plant growth regulator and the preparation process thereof. To solve the problem that the prior art is not ideal in the aspects of promoting a plant to increase production and improving quality. The invention adopts the following solution : the technique for preparing the kojic acid plant growth regulator comprises the following steps: step 1, preparing liquid fermentation medium by glucose, potassium phosphate dibasic, potassium chloride, magnesium sulphate, ferrous sulfate and ammonium nitrate, adding aspergillus by aseptic operation to ferment spawn, stirring, and fermenting continuously; step 2, heating the mature fermented liquid for carrying out solid-liquid separation to obtain a transparent liquid in yellow to tan; and step 3, decompressing and concentrating to obtain a brown liquid preparation. Compared with the prior art, the kojic acid plant growth regulator and the preparation process thereof which adopt microbe fermentation production process have the advantages that the emphytic character of the produced spawn is stable; the product has a definite formula and functional components; and obvious effects on production increasing and quality improving after vegetable spraying facilities.
Description
Technical field:
The invention belongs to microbial fermentation product and preparation method thereof technical field, be specifically related to a kind of kojic acid plant growth regulator and preparation technology thereof.
Background technology:
Kojic acid is a kind of Acidic Compounds that utilizes starch sugar raw material aerobic fermentation to produce by microorganism, chemical name 2-methylol-5-hydroxyl-gamma-pyrone, its structure is similar to glucose, confirms through isotope experiment, and the biosynthesis pathway of kojic acid is to be formed by glucose direct oxidation dehydration.Chemical composition and molecular structure according to kojic acid, that kojic acid has is antibacterial, anti-oxidant, suppress the character such as polyphenol oxidase, chelated metal ions, can be used as the antibiotic antiseptic of food, fruits and vegetables prevent the antistaling agent of enzymatic browning, the antioxidation and color-protecting agent of meat products, aquatic products, the brightening, dispelling spots, dispelling function base-material of skin-lightening cosmetic.Therefore, can see that kojic acid has application prospect preferably in industries such as food processing, garden stuff processing, medical material production, cosmetics, be the particularly comparatively active a kind of organic acid of product application and development of Recent study, and its application is constantly developed.The organic acid plant growth regulator is widely used in agricultural production, but prior art is promoting crop yield and improving aspect quality not ideal enough.
Summary of the invention:
The invention provides a kind of kojic acid plant growth regulator and preparation technology thereof, promoting crop yield and improving undesirable problem aspect quality to solve prior art.
The problem that exists for solving prior art, technical solution of the present invention is:
A kind of kojic acid plant growth regulator is prepared from by following method:
Step 1, fermentation: the part by weight by glucose 10～12%, dipotassium hydrogen phosphate 0.05～0.1%, potassium chloride 0.025～0.05%, magnesium sulfate 0.025～0.05%, ferrous sulfate 0.001～0.002%, yeast extract 0.1～0.2%, ammonium nitrate 0.2～0.4% prepares liquid fermentation medium, routine operation is to the fermentation medium sterilization treatment, 6～10% sterile workings on weight of solution access the aspergillus fermented bacteriums, 30 ± 1 ℃ of steady temperatures, mixing speed 150～200rpm/min, ventilation 0.27～0.3M
3/ M
3Min, tank pressure 0.065～0.07MPa, continuing fermentation stop fermentation when measuring zymotic fluid kojic acid content and reach 3～5g/100ml;
Step 2 is extracted and is separated: maturing fermentation liquid is heated to 80～85 ℃, kept 15～20 minutes, carry out Separation of Solid and Liquid, obtain yellow to brown yellow transparent liquid;
Step 3, preparation is concentrated: filtrate is at 60～70 ℃, and vacuum 13～14KPa is evaporated to 50% volume, obtains the brown liquid preparation.
A kind of preparation method of kojic acid plant growth regulator comprises the steps successively,
Step 1, fermentation: the part by weight by glucose 10～12%, dipotassium hydrogen phosphate 0.05～0.1%, potassium chloride 0.025～0.05%, magnesium sulfate 0.025～0.05%, ferrous sulfate 0.001～0.002%, yeast extract 0.1～0.2%, ammonium nitrate 0.2～0.4% prepares liquid fermentation medium, routine operation is to the fermentation medium sterilization treatment, 6～10% sterile workings on weight of solution access the aspergillus fermented bacteriums, 30 ± 1 ℃ of steady temperatures, mixing speed 150～200rpm/min, ventilation 0.27～0.3M
3/ M
3Min, tank pressure 0.065～0.07MPa, continuing fermentation stop fermentation when measuring zymotic fluid kojic acid content and reach 3～5g/100ml;
Step 2 is extracted and is separated: maturing fermentation liquid is heated to 80～85 ℃, kept 15～20 minutes, carry out Separation of Solid and Liquid, obtain yellow to brown yellow transparent liquid;
Step 3, preparation is concentrated: filtrate is at 60～70 ℃, and vacuum 13～14KPa is evaporated to 50% volume, obtains the brown liquid preparation.
The present invention's advantage compared with prior art is:
The present invention is take glucose as raw material, auxiliary dipotassium hydrogen phosphate, potassium chloride, magnesium sulfate, ferrous sulfate, yeast extract and the ammonium nitrate of joining, be mixed with the nutrient medium that contains various ingredients, be converted into kojic acid plant growth regulator through the micro-organisms aspergillus strain fermentation, this kojic acid plant growth regulator can promote crop yield, improve quality.The present invention adopts the microbial fermentation production technology, has definite technological process and process operation parameter, produces the bacterial classification hereditary capacity and stablizes, and product is comprised of and functional component clear and definite formula, after facilities vegetable sprays, obvious volume increase and the effect that improves quality is arranged.
Embodiment:
The below will explain the present invention by specific embodiment.
Embodiment 1:
A kind of kojic acid plant growth regulator is prepared from by following method:
Step 1: press the total ingredients by weight obtaining liq of 10kg fermentation medium, glucose 1.2kg wherein, dipotassium hydrogen phosphate 0.01kg, potassium chloride 0.005kg, magnesium sulfate 0.005kg, ferrous sulfate 0.0002kg, yeast extract 0.02kg, ammonium nitrate 0.04kg, surplus is water.Be 5.0 with the sulphur acid for adjusting pH, be heated to 120 ℃, constant 30 minutes, routine operation sterilization treats that the medium temperature is down to 30 ℃, access 1kg kojic acid fermented liquid bacterial classification, 30 ± 1 ℃ of steady temperatures, mixing speed is controlled 150rpm/min at 1～72hr, controls 200rpm/min at 73～120hr, and ventilation is controlled 0.27M at 1～72hr
3/ M
3Min controls 0.3M at 73～120hr
3/ M
3Min, tank pressure is controlled 0.065MPa at 1～72hr, controls 0.07MPa at 73～120hr, and continuing fermentation 120hr begins to measure zymotic fluid kojic acid content, namely stops fermentation when reaching 5g/100ml.
Step 2, extract and separate: maturing fermentation liquid is heated to 80～85 ℃, keeps 15 minutes, obtains brown color filtrate with the press filtration mode,
Step 3, preparation is concentrated: use decompression concentrator, under the condition of 60～70 ℃ of temperature, vacuum 13KPa, reduced pressure concentration obtains the 5.0kg preparation.
Embodiment 2:
A kind of kojic acid plant growth regulator is prepared from by following method:
Step 1: press the total ingredients by weight obtaining liq of 50kg fermentation medium, glucose 5.0kg wherein, dipotassium hydrogen phosphate 0.025kg, potassium chloride 0.0125kg, magnesium sulfate 0.0125kg, ferrous sulfate 0.0005kg, yeast extract 0.05kg, ammonium nitrate 0.1kg, surplus is water.Be 5.0 with the sulphur acid for adjusting pH, be heated to 120 ℃, constant 30 minutes, routine operation sterilization treats that the medium temperature is down to 30 ℃, access 4kg kojic acid fermented liquid bacterial classification, 30 ± 1 ℃ of steady temperatures, mixing speed is controlled 150rpm/min at 1～72hr, controls 200rpm/min at 73～120hr, and ventilation is controlled 0.27M at 1～72hr
3/ M
3Min controls 0.3M at 73～120hr
3/ M
3Min, tank pressure is controlled 0.065MPa at 1-72hr, controls 0.07MPa at 73-120hr, and continuing fermentation 120hr begins to measure zymotic fluid kojic acid content, namely stops fermentation when reaching 4g/100ml.
Step 2, extract and separate: maturing fermentation liquid is heated to 80～85 ℃, keeps 20 minutes, obtains brown color filtrate with the press filtration mode;
Step 3, preparation is concentrated: use decompression concentrator, under the condition of 60～70 ℃ of temperature, vacuum 14KPa, reduced pressure concentration obtains the 25kg preparation.
Embodiment 3:
A kind of kojic acid plant growth regulator is prepared from by following method:
Step 1: press the total ingredients by weight obtaining liq of 10kg fermentation medium, glucose 1.1kg wherein, dipotassium hydrogen phosphate 0.01kg, potassium chloride 0.0025kg, magnesium sulfate 0.005kg, ferrous sulfate 0.0002kg, yeast extract 0.01kg, ammonium nitrate 0.04kg, surplus is water.Be 5.0 with the sulphur acid for adjusting pH, be heated to 140 ℃, constant 25 minutes, routine operation sterilization treats that the medium temperature is down to 30 ℃, access 0.8kg kojic acid fermented liquid bacterial classification, 30 ± 1 ℃ of steady temperatures, mixing speed is controlled 150rpm/min at 1～72hr, controls 200rpm/min at 73～120hr, and ventilation is controlled 0.27M at 1～72hr
3/ M
3Min controls 0.3M at 73～120hr
3/ M
3Min, tank pressure is controlled 0.065MPa at 1-72hr, controls 0.07MPa at 73-120hr, and continuing fermentation 120hr begins to measure zymotic fluid kojic acid content, namely stops fermentation when reaching 5g/100ml.
Step 2, extract and separate: maturing fermentation liquid is heated to 80～85 ℃, keeps 20 minutes, obtains brown color filtrate with the press filtration mode,
Step 3, preparation is concentrated: use decompression concentrator, under the condition of 60～70 ℃ of temperature, vacuum 13KPa, reduced pressure concentration obtains the 5.0kg preparation.
Using method: the said goods is soaked seed or be sprayed onto vegetable surface after the water dilution and use as plant growth regulator.As 10 times of dilute with waters, soaked wheat, corn, soybean, cucumber seed kind 30 minutes, can effectively improve the germination rate of above-mentioned seed kind, increase plant height, root length, bright root heavily reach bright cauline leaf and weigh, and as with after 0.75%, 0.375% kojic acid plant growth regulator seed soaking cucumber, grow 20 days, average plant height is respectively 16.8cm and 16.6cm, that clear water contrasts is 6.3cm, and root length is respectively 5.7cm and 5.3cm, and contrasts only 4.77cm.As 15 times of dilute with waters, spray greenhouse cucumber, can shorten seedling-slowing stage, promote the cucumber seedling growing way, increase plant height, the number of blade, as with 0.5%, 0.25% kojic acid plant growth regulator to the cucumber plant foliar spray, be conducive to vertical slow seedling, the shortening seedling-slowing stage of planting of cucumber.After using 3 times continuously, the cucumber seedling growing way of 0.5% kojic acid preparation treatment region is best, and plant height contrasts increases 13.5-25cm, and the number of blade increases the 1-3 sheet, and the leaf look dark green, and growth potential is strong.This product has Promoting plant growth grows, and improves the effect of quality, can and be applied to agricultural production as seed soaking agent, the foliage-spray of crops.
Claims (2)
1. kojic acid plant growth regulator is prepared from by following method:
Step 1: press the total ingredients by weight obtaining liq of lOkg fermentation medium, glucose 1.1kg wherein, dipotassium hydrogen phosphate 0.01kg, potassium chloride 0.0025kg, magnesium sulfate 0.005kg, ferrous sulfate 0.0002kg, yeast extract 0.01kg, ammonium nitrate 0.04kg, surplus is water; Be 5.0 with the sulphur acid for adjusting pH, be heated to 140 ℃, constant 25 minutes, routine operation sterilization treats that the medium temperature is down to 30 ℃, access 0.8kg kojic acid fermented liquid bacterial classification, 30 ± 1 ℃ of steady temperatures, mixing speed is controlled 150rpm/min at l-72hr, controls 200rpm/min at 73-120hr, and ventilation is controlled 0.27M at 1-72hr
3/ M
3Min controls 0.3 M at 73-120hr
3/ M
3Min, tank pressure is controlled 0.065MPa at 1-72hr, controls 0.07MPa at 73-120hr, and continuing fermentation 120hr begins to measure zymotic fluid kojic acid content. namely stop fermentation when reaching 5g/lOOml;
Step 2, extract and separate: maturing fermentation liquid is heated to 80-85 ℃, keeps 20 minutes, obtains brown color filtrate with the press filtration mode;
Step 3, preparation is concentrated: use decompression concentrator, under the condition of 60-70 ℃ of temperature, vacuum 13KPa, reduced pressure concentration obtains the 5.0kg preparation.
2. as the preparation method of the described a kind of kojic acid plant growth regulator of claim l, comprise the steps successively,
Step 1: press the total ingredients by weight obtaining liq of lOkg fermentation medium, glucose 1.1kg wherein, dipotassium hydrogen phosphate 0.01kg, potassium chloride 0.0025kg, magnesium sulfate 0.005kg, ferrous sulfate 0.0002kg, yeast extract 0.01kg, ammonium nitrate 0.04kg, surplus is water; Be 5.0 with the sulphur acid for adjusting pH, be heated to 140 ℃, constant 25 minutes, routine operation sterilization treats that the medium temperature is down to 30 ℃, access 0.8kg kojic acid fermented liquid bacterial classification, 30 ± 1 ℃ of steady temperatures, mixing speed is controlled 150rpm/min at l-72hr, controls 200rpm/min at 73-120hr, and ventilation is controlled 0.27M at 1-72hr
3/ M
3Min controls 0.3 M at 73-120hr
3/ M
3Min, tank pressure is controlled 0.065MPa at 1-72hr, controls 0.07MPa at 73-120hr, and continuing fermentation 120hr begins to measure zymotic fluid kojic acid content. namely stop fermentation when reaching 5g/lOOml;
Step 2, extract and separate: maturing fermentation liquid is heated to 80-85 ℃, keeps 20 minutes, obtains brown color filtrate with the press filtration mode;
Step 3, preparation is concentrated: use decompression concentrator, under the condition of 60-70 ℃ of temperature, vacuum 13KPa, reduced pressure concentration obtains the 5.0kg preparation.
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| CN102321700A (en) * | 2011-08-03 | 2012-01-18 | 无锡赛德生物工程有限公司 | Kojic acid fermentation technique and apparatus |
| CN107006167A (en) * | 2017-03-17 | 2017-08-04 | 界首市兴邦家庭农场 | A kind of method for culturing seedlings of soya seeds bud rate occurred frequently |
| CN113481016A (en) * | 2021-07-29 | 2021-10-08 | 海南金象云农业科技有限公司 | Soil conditioner and preparation method and application thereof |
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Non-Patent Citations (2)
| Title |
|---|
| 王博彦, 等.发酵有机酸生产与应用手册.《发酵有机酸生产与应用手册》.中国轻工业出版社,2000,第598-603页. * |
| 陆正清,等.曲酸的发酵法生产及其在食品加工中的应用.《中国调味品》.2008,(第01期), * |
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