CN101435751A - Method and apparatus for collecting water sample in aquipectenirradiuns cultivation cage - Google Patents
Method and apparatus for collecting water sample in aquipectenirradiuns cultivation cage Download PDFInfo
- Publication number
- CN101435751A CN101435751A CNA2008102385380A CN200810238538A CN101435751A CN 101435751 A CN101435751 A CN 101435751A CN A2008102385380 A CNA2008102385380 A CN A2008102385380A CN 200810238538 A CN200810238538 A CN 200810238538A CN 101435751 A CN101435751 A CN 101435751A
- Authority
- CN
- China
- Prior art keywords
- cage
- scallop
- sampling
- stopple coupon
- water sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention provides a method and a device for collecting a water sample in a scallop culturing cage. The method is characterized by comprising: a sampling pipe is inserted in the cage through a hole of a base plate of the scallop cage, and is arranged in the center of the scallop cage; the other end of the sampling pipe is fixed on a buoyant raft of a culturing raft rack; sampling is started after adaption of between 3 and 5 days; one end of the sampling pipe is taken off from the buoyant raft, and is closely connected with a sampling pipe connection plug on a rubber plug of a sampling bottle mouth; a vacuumizing pipe of a manual vacuum pump is closely connected with a vacuumizing pipe connection plug on the rubber plug of the sampling bottle mouth; water in the sampling pipe is pumped and poured first, and then is vacuumized by the manual vacuum pump, so that water sucked in the sampling bottle can be taken to a laboratory for analyzing treatment after reaching a required amount. The method can collect on-site water sample without interrupting cultured creatures, thereby ensuring scientificity and effectiveness of marine ecologic environmental monitoring.
Description
Technical field
The invention belongs to breeding ecological learn in the manufacturing technology field of relevant ECOLOGICAL ENVIRONMENTAL MONITORING sample collecting method and sample apparatus equipment, be the acquisition method and the device of water sample in a kind of scallop cultivation cage specifically, the mensuration that is used for the physics and chemistry coenocorrelation factors such as water body chlorophyll, suspended particulate matter concentration, microorganism and nutrient concentration in the cylinder mould, analyze and research the in view of the above variation of the inside and outside ecologic environment of cylinder mould is estimated fouling organism on cultured scallop and the cylinder mould to the influence of environment in the cylinder mould.
Background technology
Fouling organism in the raft culture process becomes international, ubiquity and the problem that faces, has caused to pay close attention to widely and pay attention to greatly.Fouling organism is attached on cylinder mould etting, cylinder mould dividing plate and the shell in a large number, block mesh, influence the inside and outside water quality exchange of cylinder mould, strive food with aquaculture organism and take bait by force, the weight of equipment is cultured in aging, the increasing of quickening cylinder mould, hinder the growth of aquaculture organism, reduce quality of aquatic products or the like, thereby influence the production efficiency of aquaculture and output, the output value.Especially along with the continuous expansion of China's raft culture scale and cultivation density, aquaculture organism, raft frame, fouling organism etc. hinder the exchange of current, aquaculture organism is for the bait deficiency, and the refuse of metabolism can not in time spread, and local waters environmental baseline worsens phenomenon and is on the rise.Since 1997, it is dead on a large scale that northern China is cultured Chlamys farreri, brought enormous economic loss to China.So far, the Chlamys farreri aquaculture still fails to rise from the valley bottom.Result of study thinks that it is the extensive main causes of death of Chlamys farreri that harmful growing of pathogen induced in the deterioration of breeding environment.PRELIMINARY RESULTS shows, the adhering to the busy season of the emergence period of Chlamys farreri high mortality and fouling organism (in 7-9 month) is extremely identical, and growth rate, the mortality ratio that fan in the biomass of fouling organism and comb hole have good correlativity.Has is " little ecology " environment in the shellfish culture cylinder mould tarnished biological influence and significant change taken place? fouling organism is to the variation of understanding fully " little ecology " environment in the cylinder mould of aquaculture organism growth, survival, can be used as potential diagnostic means and cause the extensive dead condition of cultivated shellfish with judgement, may aspect the reason of inquiring into the extensive death of Chlamys farreri, mechanism, make a breakthrough.
At present, some fouling organisms do not have the variation of " little ecology " environment in the quantitative research cylinder mould to the many suppositions based on qualitative or science of the influence of aquaculture.Because the measurement difficulty of water environment is bigger in the cylinder mould, about in the research process of fouling organism to the influence of " little ecology " environment in the shellfish cylinder mould, is to carry out each parametric measurement by the water sample that the frogman obtains in the cylinder mould so far.Also there are not to reduce sample apparatus equipment and acquisition method now to the disturbance of " little ecology " environment in the cylinder mould.
Summary of the invention
The objective of the invention is to exist expense height, efficient low at existing (frogman's diving) sampling method, the problem that sampling technique very lacks, the acquisition method and the device of water sample in a kind of scallop cultivation cage are provided, and sampling method is simple, cost is low, efficient is high; Sampler is simple and reasonable for structure, and field investigation is easy to carry, can reduce the disturbance to " little ecology " environment in the cylinder mould, applied range.
The objective of the invention is to be achieved through the following technical solutions: the acquisition method of water sample in a kind of scallop cultivation cage is characterized in that the water sampling method is as follows:
Stopple coupon is inserted in the cage by the hole on the scallop cage chassis, stopple coupon is placed the central authorities of scallop cage, the other end of stopple coupon is fixed on the buoyant raft of culture raft frame, adapt to and begin sampling after 3～5 days, one end of stopple coupon is taken off from buoyant raft, closely link to each other with the stopple coupon attachment plug beyond the Great Wall of sampling jar chewing-gum, closely link to each other with the vacuum-pumping tube attachment plug beyond the Great Wall of sampling jar chewing-gum with the hand-operating vacuum-pump vacuum-pumping tube, vacuumize the inner all water of extraction stopple coupon by hand-operating vacuum-pump, be used for the flushing of sampling jar, outwell, begin to gather water sample, vacuumize with hand-operating vacuum-pump, the water sample that sucks in the sampling jar is reached to take back the laboratory behind the aequum and carry out analyzing and processing.
Improvement to above-mentioned acquisition method: stopple coupon inserts 0.4～0.6m in the scallop cage through 3 scallop cage chassis, and it is 2.5～3.5m that the scallop cage hangs the foster depth of water.
Further improvement to above-mentioned acquisition method: experimental needs, in 3～5 scallop cages, insert stopple coupon, establish parallel sample.
The harvester of water sample in a kind of scallop cultivation cage, it is characterized in that, comprise the stopple coupon attachment plug and the vacuum-pumping tube attachment plug that penetrate on plug on hand-operating vacuum-pump, stopple coupon, sampling jar, the sampling bottleneck, the plug, the hand-operating vacuum-pump vacuum-pumping tube closely links to each other with vacuum-pumping tube attachment plug, stopple coupon one end closely links to each other with stopple coupon attachment plug, and the other end inserts in the cage by the hole on the scallop cage chassis.
Improvement to above-mentioned harvester: described stopple coupon attachment plug is the glass tube of external diameter 10mm, and described vacuum-pumping tube attachment plug is the glass tube of external diameter 8mm, and the sampling jar volume is 500ml～1000ml.
Further improvement to above-mentioned harvester: described hand-operating vacuum-pump max vacuum is pressed and is not less than 635 mm Hg.
Further improvement to above-mentioned harvester: the material of sampling jar adopts polyethylene bottle or vial.
Further improvement to above-mentioned harvester: described plug external diameter is suitable with the internal diameter of sampling bottleneck, and plug is filled in closely on the sampling bottleneck, so that whole device can be in air-tight state, is convenient to the absorption of water sample.
Further improvement to above-mentioned harvester: described stopple coupon is a polyethylene pipe, and the internal diameter of stopple coupon is 10mm, and external diameter is 12mm.
The present invention compared with prior art has many advantages and good effect:
1, the acquisition method of water sample in the scallop cultivation cage of the present invention can " micro-ecological environment " cause under the situation of disturbance in not to aquaculture organism and cylinder mould, obtains on-the-spot water sample, helps to understand the real situation of water quality in the scallop cage, reduces the error of experiment.Sampling method is simple, cost is low, efficient is high.
2, the required material of harvester of water sample is simple and easy in the scallop cultivation cage of the present invention, and cost is low, and field stripping was convenient when apparatus was at sea investigated, and is light flexibly, simple to operate, quick, only need can finish operating personnel's simple training.
3, the present invention can be according to the purpose of research and the needs of the water quality parameter of mensuration, the sampling jar of transposing unlike material.
4, the present invention also can be used for water quality environment investigation, sampling in the fish net cage.
Description of drawings
Fig. 1 is the structural drawing of the harvester of water sample in a kind of scallop cultivation cage of the present invention.
Embodiment
As shown in Figure 1, embodiment for the harvester of water sample in a kind of scallop cultivation cage of the present invention, comprise the stopple coupon attachment plug 4 and the vacuum-pumping tube attachment plug 5 that penetrate on plug 3 on hand-operating vacuum-pump 1, stopple coupon 6, sampling jar 2, sampling jar 2 bottlenecks, the plug 3, hand-operating vacuum-pump 1 closely links to each other with vacuum-pumping tube attachment plug 5 by vacuum-pumping tube 7, stopple coupon 6 one ends closely link to each other with stopple coupon attachment plug 4, and the other end inserts in the cage by the hole on the scallop cage chassis.
The NALGENE board L222243 type that described hand-operating vacuum-pump 1 selects for use the U.S. to produce is furnished with pressure vacuum gauge, and the each 36cm3 of suction filtration speed reaches as high as 635 mm Hg vacuum tightnesss.
Described sampling jar 2 is cylindrical wide-mouth vial or the polyethylene bottle of volume 500ml～1000ml, and internal diameter of the bottleneck is 43m.The material of sampling jar 2 is to select according to the environmental index of measuring, and for example, the index of mensuration is nutrient concentrations such as the dissolubility inorganic nitrogen, reactive phosphate in the water body, and sampling jar 2 is selected vial or polyethylene bottles.
The external diameter of described plug 3 is 43mm, makes a call to two through holes on plug 3, and the aperture is respectively 8mm and 10mm.
Described stopple coupon attachment plug 4 is the glass tube of 10mm for external diameter, and vacuum-pumping tube attachment plug 5 is the glass tube of 8mm for external diameter, and two glass tubes 4,5 tightly insert two corresponding on the plug 3 through holes, and glass tube 4,5 is reserved the length of 20mm outside plug 3.
The internal diameter of described stopple coupon 6 is 10mm, and external diameter is 12mm, and stopple coupon 6 external diameters are consistent with the hole on the scallop cultivation cage chassis, so that stopple coupon is inserted in the scallop cultivation cage securely.
Be described in detail the acquisition method of water sample in a kind of scallop cultivation cage of the present invention below by example:
During water sample in the collection in worksite cultured scallop cage, as required, stopple coupon 6 is inserted in the cage by the hole on the scallop cage chassis,, stopple coupon is placed central authorities' (approximately inserting cage 0.5m) of scallop cage through 3 chassis.The other end of stopple coupon 6 is fixed on the buoyant raft of culture raft frame.It is about 3m that the scallop cage hangs the foster depth of water.Adapt to after 3～5 days, begin sampling.According to the length of stopple coupon 6, calculating the water that retains in the stopple coupon 6 is 275ml.One end of stopple coupon 6 is taken off from buoyant raft, closely link to each other, hand-operating vacuum-pump 1 is closely linked to each other with vacuum-pumping tube attachment plug 5 by vacuum-pumping tube 7 with stopple coupon attachment plug 4, vacuumize with hand-operating vacuum-pump 1, extract water 300ml out, be used for the flushing of sampling jar 2, outwell, begin to gather water sample.If the water sample deficiency of Xi Quing contains the water that remains in for a long time in the stopple coupon 6 in the water sample of being gathered in advance, can not reflect the environmental quality in the scallop cultivation cage really, can make the data of experiment lose true and accurate.According to the needs of measuring environmental parameter, pump is got the seawater 500ml in the cultured scallop cage, is used for the mensuration of seawater chlorophyll-a concentration.Water sample is taken back the laboratory carry out analyzing and processing.
Also can take an amount of water sample of above-mentioned acquisition mode collection, carry out the mensuration of the physics and chemistry coenocorrelation factors such as suspended particulate matter concentration, microorganism and nutrient concentration.Analyze and research the in view of the above variation of the inside and outside ecologic environment of cylinder mould is estimated fouling organism on cultured scallop and the cylinder mould to the influence of environment in the cylinder mould.
Can experimental needs, in 3～5 scallop cages, insert stopple coupon 6, establish parallel sample.
In sum, use the acquisition method and the device of water sample in the scallop cultivation cage of the present invention can gather the interior water sample of cultured scallop cage easily, measure the variation of the every index of water quality environment in the cage, variation and the scallop of understanding water environment in the cylinder mould grown, the possible internal relation of survival.Have characteristics such as the combination dismounting is simple and direct, execute-in-place is strong, sampling efficiency is high, usable range is extensive.Point out that at this acquisition method of water sample is similar in on-site measurement method that is used for the aspects such as collection of water sample in the fish net cage and the scallop cage, measures the example of studying and omits at this.
Certainly, above-mentioned explanation is not to be limitation of the present invention, and the present invention is not limited to above-mentioned giving an example, those skilled in the art, and in essential scope of the present invention, the variation of making, remodeling, interpolation or replacement all should belong to protection scope of the present invention.
Claims (10)
1, the acquisition method of water sample in a kind of scallop cultivation cage is characterized in that the water sampling method is as follows:
Stopple coupon is inserted in the cage by the hole on the scallop cage chassis, stopple coupon is placed the central authorities of scallop cage, the other end of stopple coupon is fixed on the buoyant raft of culture raft frame, adapt to and begin sampling after 3～5 days, one end of stopple coupon is taken off from buoyant raft, closely link to each other with the stopple coupon attachment plug beyond the Great Wall of sampling jar chewing-gum, closely link to each other with the vacuum-pumping tube attachment plug beyond the Great Wall of sampling jar chewing-gum with the hand-operating vacuum-pump vacuum-pumping tube, vacuumize the inner all water of extraction stopple coupon by hand-operating vacuum-pump, be used for the flushing of sampling jar, outwell, begin to gather water sample, vacuumize with hand-operating vacuum-pump, the water sample that sucks in the sampling jar is reached to take back the laboratory behind the aequum and carry out analyzing and processing.
According to the acquisition method of water sample in the described scallop cultivation cage of claim 1, it is characterized in that 2, stopple coupon inserts 0.4～0.6m in the scallop cage through 3 scallop cage chassis, it is 2.5～3.5m that the scallop cage hangs the foster depth of water.
3, according to the acquisition method of water sample in claim 1 or the 2 described scallop cultivation cages, it is characterized in that, in 3～5 scallop cages, insert stopple coupon, establish parallel sample.
4, the harvester of water sample in a kind of scallop cultivation cage, it is characterized in that, comprise the stopple coupon attachment plug and the vacuum-pumping tube attachment plug that penetrate on plug on hand-operating vacuum-pump, stopple coupon, sampling jar, the sampling bottleneck, the plug, the hand-operating vacuum-pump vacuum-pumping tube closely links to each other with vacuum-pumping tube attachment plug, stopple coupon one end closely links to each other with stopple coupon attachment plug, and the other end inserts in the cage by the hole on the scallop cage chassis.
5, according to the harvester of water sample in the described scallop cultivation cage of claim 4, it is characterized in that, described stopple coupon attachment plug is the glass tube of external diameter 10mm, and described vacuum-pumping tube attachment plug is the glass tube of external diameter 8mm, and the sampling jar volume is 500ml～1000ml.
According to the harvester of water sample in claim 4 or the 5 described scallop cultivation cages, it is characterized in that 6, described hand-operating vacuum-pump max vacuum is pressed and is not less than 635 mm Hg.
According to the harvester of water sample in claim 4 or the 5 described scallop cultivation cages, it is characterized in that 7, the material of described sampling jar adopts polyethylene bottle or vial.
According to the harvester of water sample in claim 4 or the 5 described scallop cultivation cages, it is characterized in that 8, described plug external diameter is suitable with the internal diameter of sampling bottleneck, plug is filled in closely on the sampling bottleneck, so that whole device can be in air-tight state.
According to the harvester of water sample in the described scallop cultivation cage of claim 7, it is characterized in that 9, described plug external diameter is suitable with the internal diameter of sampling bottleneck, plug is filled in closely on the sampling bottleneck, so that whole device can be in air-tight state.
10, according to the harvester of water sample in claim 4 or the 5 described scallop cultivation cages, it is characterized in that described stopple coupon is a polyethylene pipe, the internal diameter of stopple coupon is 10mm, and external diameter is 12mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102385380A CN101435751B (en) | 2008-12-18 | 2008-12-18 | Method and apparatus for collecting water sample in aquipectenirradiuns cultivation cage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102385380A CN101435751B (en) | 2008-12-18 | 2008-12-18 | Method and apparatus for collecting water sample in aquipectenirradiuns cultivation cage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101435751A true CN101435751A (en) | 2009-05-20 |
| CN101435751B CN101435751B (en) | 2011-02-02 |
Family
ID=40710303
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008102385380A Expired - Fee Related CN101435751B (en) | 2008-12-18 | 2008-12-18 | Method and apparatus for collecting water sample in aquipectenirradiuns cultivation cage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101435751B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103926113A (en) * | 2014-04-10 | 2014-07-16 | 浙江省海洋水产研究所 | Sterile water sample collecting bottle for detection |
| CN104293658A (en) * | 2014-10-03 | 2015-01-21 | 浙江大学舟山海洋研究中心 | Sterile metering sampler for fermentation and use of sterile metering sampler |
| CN105403430A (en) * | 2015-12-11 | 2016-03-16 | 苏州国环环境检测有限公司 | A water sampling device |
| CN106353138A (en) * | 2016-09-06 | 2017-01-25 | 天津渤海水产研究所 | Water sample and bottom sediment collecting device for bottom water course coupled interface |
| CN110672383A (en) * | 2019-11-21 | 2020-01-10 | 上海美旗环保技术工程有限公司 | Negative pressure water absorption type automatic sampler |
| CN113514281A (en) * | 2021-04-26 | 2021-10-19 | 上海化工院检测有限公司 | Sampling device for residual liquid in barrel |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102042916B (en) * | 2009-10-15 | 2013-04-24 | 贝荣辉 | Ship ballast water collection device |
-
2008
- 2008-12-18 CN CN2008102385380A patent/CN101435751B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103926113A (en) * | 2014-04-10 | 2014-07-16 | 浙江省海洋水产研究所 | Sterile water sample collecting bottle for detection |
| CN104293658A (en) * | 2014-10-03 | 2015-01-21 | 浙江大学舟山海洋研究中心 | Sterile metering sampler for fermentation and use of sterile metering sampler |
| CN105403430A (en) * | 2015-12-11 | 2016-03-16 | 苏州国环环境检测有限公司 | A water sampling device |
| CN106353138A (en) * | 2016-09-06 | 2017-01-25 | 天津渤海水产研究所 | Water sample and bottom sediment collecting device for bottom water course coupled interface |
| CN106353138B (en) * | 2016-09-06 | 2024-01-12 | 天津市水产研究所 | Water sample and bottom mud collecting device of bottom water layer coupling interface |
| CN110672383A (en) * | 2019-11-21 | 2020-01-10 | 上海美旗环保技术工程有限公司 | Negative pressure water absorption type automatic sampler |
| CN113514281A (en) * | 2021-04-26 | 2021-10-19 | 上海化工院检测有限公司 | Sampling device for residual liquid in barrel |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101435751B (en) | 2011-02-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101435751B (en) | Method and apparatus for collecting water sample in aquipectenirradiuns cultivation cage | |
| Choi | Primary production and release of dissolved organic carbon from phytoplankton in the western North Atlantic Ocean | |
| CN201883097U (en) | Low-temperature nitrobacteria culture box and culture device | |
| CN202143383U (en) | In-situ cultivation barrel for phytoplankton | |
| Alongi | Extraction of protists in aquatic sediments via density gradient centrifugation | |
| CN111707491B (en) | Device and method for sampling biological membrane in underground water | |
| CN207081564U (en) | Raw biological sampling device | |
| CN105475189B (en) | Individual marking method in echinoderm BLUP method breeding process | |
| CN213632775U (en) | A high-efficient sampler of water for determining water vibrio | |
| CN106359237B (en) | Marine shellfish biological physiological experiment device and method based on community level | |
| CN216585031U (en) | Zebra fish gradient dissolved oxygen experimental apparatus | |
| CN103404464B (en) | A kind of bioscrubbing method of fouling organism on mariculture scallop cage and shell | |
| Davis et al. | A mesocosm system for ecological research with marine invertebrate larvae | |
| CN210834298U (en) | Marine pollution sampling equipment | |
| CN115165454A (en) | Glass fiber membrane-based eDNA solid passive sampler and passive sampling method | |
| CN109061069A (en) | A kind of snail and fish eco-toxicity in-situ monitoring device and detection method | |
| CN209947207U (en) | Large-space-scale field portable micro-universe experimental device | |
| Carter et al. | Investigation into the use of Zostera muelleri (Irmisch ex Aschers) as a sentinel accumulator for copper | |
| IMAI et al. | A simple technique for establishing axenic cultures of phytoflagellates | |
| CN202230011U (en) | Grading and counting device for living microalgae in ballast water | |
| CN213239498U (en) | Netting stained organism collecting, counting and storing device | |
| CN215346520U (en) | Oyster hypoxemia stress experimental apparatus | |
| CN203132868U (en) | Sampler for aquaculture | |
| CN204762892U (en) | Cultivate nemic separation collection device in ocean | |
| CN214702858U (en) | Undisturbed water sample collector |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110202 Termination date: 20111218 |