CN215346520U - Oyster hypoxemia stress experimental apparatus - Google Patents

Oyster hypoxemia stress experimental apparatus Download PDF

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Publication number
CN215346520U
CN215346520U CN202120549104.3U CN202120549104U CN215346520U CN 215346520 U CN215346520 U CN 215346520U CN 202120549104 U CN202120549104 U CN 202120549104U CN 215346520 U CN215346520 U CN 215346520U
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oxygen
barrel body
oyster
barrel
tank
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马元
韦嫔媛
何苹萍
彭金霞
张兴志
官俊良
张立
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Guangxi Academy of Fishery Sciences
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Guangxi Academy of Fishery Sciences
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Abstract

The utility model discloses an oyster hypoxia stress experimental device which comprises a barrel body, a filter, a supporting frame, an air pump, an oxygen dissolving instrument, a wave making pump, a barrel cover, a nitrogen tank and an oxygen tank, wherein the nitrogen tank and the oxygen tank are arranged on one side of the barrel body; one side of the bottom end in the barrel body is respectively provided with a bubble bar and a heating rod, and the other side of the bottom end in the barrel body is provided with a filter; the nitrogen tank and the oxygen tank are respectively connected with the air inlet of the air pump through a conduit, and the air outlet of the air pump is connected with the air inlet of the bubble strip. According to the utility model, a closed system is adopted to perform an oyster hypoxia stress test, so that the influence of the entering of external oxygen on the experimental result is prevented; the flow of the nitrogen tank and the flow of the oxygen tank are controlled respectively, and then gas is output to the bubble strips through the air pump, so that the oxygen reduction and the oxygen increase of the water body are controlled, and the effects of quickly reducing the oxygen and increasing the oxygen of the water body are achieved.

Description

Oyster hypoxemia stress experimental apparatus
Technical Field
The utility model belongs to the technical field of oyster hypoxia experimental devices, and particularly relates to an oyster hypoxia stress experimental device.
Background
Dissolved oxygen is one of the essential elements for the oyster to live, and the dissolved oxygen in the water body is directly related to the life processes of ingestion, growth, reproduction and the like of the oyster. Due to global warming caused by greenhouse effect, water eutrophication, mutation or dew caused by disastrous weather and tidal action, dissolved oxygen in water can drop suddenly, oysters are stressed by hypoxia, so that the immune function is influenced, the oysters grow slowly or do not grow, and the oysters die. Therefore, the adaptation condition of the oysters to the hypoxia condition is explored under the laboratory condition, and the influence of hypoxia stress with different concentrations on the growth, survival and the like of the oysters is of great significance in guiding the proper oxygenation in the oyster cultivation process.
At present, shellfish hypoxia stress research is developed in laboratories, hypoxia experimental equipment for other aquatic animals such as fishes, shrimps, crabs and the like is mostly directly adopted, nitrogen is generally introduced into a water body to reduce dissolved oxygen content, and then a dissolved oxygen instrument is used for measuring, so that a culture container is sealed after the required dissolved oxygen concentration is reached. The hypoxia experimental device is used for oyster hypoxia stress experiments and has the following defects: 1. oysters are sessile organisms, breathing and ingestion are carried in by flowing seawater in a natural environment, and common fish, shrimp and crab hypoxia stress equipment does not have a device for stirring a water body, so that dissolved oxygen and bait algae in a culture water body are not uniformly distributed, and the hypoxia stress test result and ingestion of the oysters can be seriously influenced; 2. the air stone is adopted, so that the effect is slow in both aeration by filling air and aeration by filling nitrogen for oxygen reduction in a water body; 3. the culture water body is not provided with a filtering device, the ammonia nitrogen content is increased, the normal physiology of the oysters is influenced, and the experimental result is influenced; 4. oysters can only be tested in a mode of being paved at the bottom of a test container, and the number of the oysters tested in the mode is limited; 5. without a temperature control device, the temperature varied with the time of the experiment. Because the existing device has the defects that the accuracy of an experimental result is influenced, a hypoxia experimental device specially designed for oysters is lacked in the oyster hypoxia stress research process.
SUMMERY OF THE UTILITY MODEL
Aiming at the defects of the prior art, the utility model provides the oyster hypoxia stress experimental device which can stir water, dissolve oxygen uniformly, increase oxygen, reduce oxygen with quick effect, has a large experimental capacity and can control temperature.
In order to achieve the purpose, the technical scheme of the utility model is as follows:
an oyster hypoxia stress experimental device comprises a barrel body, a filter, a support frame, an air pump, an oxygen dissolving instrument, a wave making pump, a barrel cover, a nitrogen tank and an oxygen tank, wherein the nitrogen tank and the oxygen tank are arranged on one side of the barrel body; one side of the bottom end in the barrel body is respectively provided with a bubble bar and a heating rod, and the other side of the bottom end in the barrel body is provided with a filter; the nitrogen tank and the oxygen tank are respectively connected with the air inlet of the air pump through a conduit, and the air outlet of the air pump is connected with the air inlet of the bubble strip; the conduit connected with the nitrogen tank is provided with a flow regulating valve A, and the conduit connected with the oxygen tank is provided with a flow regulating valve B; the middle part of the barrel body is provided with an oxygen dissolving instrument, and the detection end of the oxygen dissolving instrument extends into the barrel body; a wave making pump is arranged on one side of the upper part of the barrel body, and an exhaust funnel is arranged on the other side of the upper part of the barrel body; the outlet of the exhaust funnel is provided with a sealing cover; a support frame is arranged in the middle of the inside of the barrel body; a tray body is arranged between the supporting frames; holes are uniformly distributed in the middle of the tray body; the top opening of the barrel body is connected with the barrel cover.
The filter, the dissolved oxygen meter, the wave making pump and the bubble strip are hermetically connected with the barrel body, and the sealing joints are additionally sealed by sealant; the barrel body is made of transparent acrylic or glass; the support frame set up the multilayer disk body, the disk body is equipped with the hole that runs through for dissolved oxygen volume and bait alga between every layer of oyster distribute evenly, also easily ingest oyster bait alga.
As a further technical improvement, a sealing ring is arranged between the barrel cover and the barrel body. The sealing ring further improves the sealing effect of the barrel cover and the barrel body.
As a further technical improvement, a cushion block is arranged at the bottom of the supporting frame; the bottom of cushion is equipped with the rubber pad. The bottom of support frame be equipped with the cushion, the bottom of cushion is equipped with the rubber pad, be for making the support nature of support frame better, difficult emergence is slided in the staving.
As a further technical improvement, the top of the sealing cover is hinged with the top of the exhaust funnel. The top of the sealing cover is hinged with the top of the exhaust funnel, so that the sealing cover is convenient to open and close.
As a further technical improvement, a handle is arranged in the middle of the top of the barrel cover.
As a further technical improvement, the barrel body is made of transparent acrylic or glass. The operation condition of the device in the barrel and the death condition of the oysters can be observed in the experimental process.
The use method of the utility model comprises the following steps: adding seawater crystal into a barrel body to prepare seawater, adding bait algae, wherein the height of the seawater is 5cm lower than that of an exhaust funnel, cleaning and uniformly placing healthy oyster shell surfaces with uniform specifications on a plate body, covering a barrel cover, opening a sealing cover of the exhaust funnel, respectively starting a wave making pump, an oxygen dissolving instrument and an air pump, opening a flow control valve A, closing a flow control valve B, filling nitrogen, and observing a detection value of the oxygen dissolving instrument until the concentration of dissolved oxygen is reduced to an oxygen concentration value required by an experiment; if the concentration value is lower than the concentration value, opening the flow control valve B, closing the flow control valve A, filling oxygen, and increasing the oxygen concentration value to the oxygen concentration value required by the experiment; closing the sealing cover, starting the heating rod to heat the water to the temperature specified in the experiment, starting the filter while keeping the temperature, performing an oxygen stress experiment on the oysters, observing the dissolved oxygen value displayed by the dissolved oxygen meter in real time, and timely oxygenating and recovering when the dissolved oxygen value is lower than the oxygen concentration value required by the experiment.
Compared with the prior art, the utility model has the following beneficial effects:
1. according to the utility model, a closed system is adopted to perform an oyster hypoxia stress test, so that the influence of the entering of external oxygen on the experimental result is prevented.
2. The barrel body is made of transparent acrylic or glass, and the operation condition of the device in the barrel body and the death condition of oysters can be observed in the experimental process.
3. The utility model realizes the control of oxygen reduction and oxygen increase of the water body by controlling the flow of the nitrogen tank and the oxygen tank respectively and outputting gas to the bubble strip through the air pump, thereby achieving the effect of rapid oxygen reduction and oxygen increase of the water body.
4. The utility model adds the dissolved oxygen instrument, monitors the dissolved oxygen in water in real time, reflects the dissolved oxygen condition visually, and is convenient to master and adjust the low oxygen state of the water body at any time; the control of the water body temperature is realized by adopting a heating rod heating mode.
5. The utility model adopts the mode of stirring the water body by the wave making pump, realizes uniform dissolved oxygen in the water, and is more beneficial to feeding bait algae in the water by the oysters in a running water environment.
6. The utility model realizes the purification of the water body in the barrel through the filter.
7. The openable sealing cover is arranged at the outlet of the exhaust funnel, so that gas is conveniently discharged in the oxygen adjusting process, the influence on the experimental result due to different pressures inside and outside the barrel is avoided, and the opening of the sealing cover of the experimental barrel is facilitated.
Drawings
Fig. 1 is a schematic view of the internal structure of the present invention.
FIG. 2 is a cross-sectional view of the utility model A-A.
Wherein, each icon and the corresponding part name are as follows:
1-barrel body, 2-filter, 3-disk body, 4-support frame, 5-cushion block, 6-bubble strip, 7-air pump, 8-heating rod, 9-dissolved oxygen instrument, 10-wave making pump, 11-barrel cover, 12-handle, 13-sealing ring, 14-exhaust funnel, 15-sealing cover, 16-nitrogen tank and 17-oxygen tank.
Detailed Description
In order to make the aforementioned objects, features and advantages of the basic invention comprehensible, embodiments accompanied with figures are described in further detail below.
Example 1:
as shown in fig. 1, the experimental apparatus for hypoxia stress of oysters in the embodiment comprises a barrel body 1, a filter 2, a support frame 4, an air pump 7, an oxygen dissolving instrument 9, a wave making pump 10, a barrel cover 11, a nitrogen tank 16 and an oxygen tank 17, wherein the nitrogen tank 16 and the oxygen tank are arranged on one side of the barrel body 1;
one side of the bottom end in the barrel body 1 is respectively provided with a bubble strip 6 and a heating rod 8, and the other side of the bottom end in the barrel body 1 is provided with a filter 2; the nitrogen tank 16 and the oxygen tank 17 are respectively connected with the air inlet of the air pump 7 through a conduit, and the air outlet of the air pump 7 is connected with the air inlet of the bubble bar 6; a flow regulating valve A is arranged on a conduit connected with the nitrogen tank 16, and a flow regulating valve B is arranged on a conduit connected with the oxygen tank 17; the middle part of the barrel body 1 is provided with an oxygen dissolving instrument 9, and the detection end of the oxygen dissolving instrument 9 extends into the barrel body 1; a wave making pump 10 is arranged on one side of the upper part of the barrel body 1, and an exhaust funnel 14 is arranged on the other side of the upper part of the barrel body 1; the outlet of the exhaust funnel 14 is provided with a sealing cover 15; a support frame 4 is arranged in the middle of the interior of the barrel body 1; a tray body 3 is arranged between the supporting frames 4; holes are uniformly distributed in the middle of the tray body 3; the top opening of the barrel body 1 is connected with a barrel cover 11. The filter 2, the dissolved oxygen meter 9, the wave making pump 10 and the bubble strip 6 are hermetically connected with the barrel body 1, and the sealing joints are additionally sealed by sealant; the barrel body 1 is made of transparent acrylic or glass; support frame 4 set up multilayer disk body 3, the disk body is equipped with the hole that runs through for dissolved oxygen volume and bait alga between every layer of oyster distribute evenly, also easily ingest oyster bait alga.
The application method of the embodiment comprises the following steps: adding seawater crystal into the barrel body 1 to prepare seawater, adding bait algae, wherein the height of the seawater is 5cm lower than that of the exhaust funnel, cleaning and uniformly placing healthy oyster shell surfaces of uniform specification on the plate body 3, covering the barrel cover 11, opening a sealing cover 15 of the exhaust funnel 14, respectively starting the wave making pump 10, the dissolved oxygen meter 9 and the air pump 7, opening the flow control valve A, closing the flow control valve B, filling nitrogen, and observing a detection value of the dissolved oxygen meter 9 until the dissolved oxygen concentration is reduced to an oxygen concentration value required by an experiment; if the concentration value is lower than the concentration value, opening the flow control valve B, closing the flow control valve A, filling oxygen, and increasing the oxygen concentration value to the oxygen concentration value required by the experiment; closing the sealing cover 15, starting the heating rod 8 to heat the water to the temperature specified in the experiment, starting the filter 2 while keeping the temperature, performing an oxygen stress experiment on the oysters, observing the dissolved oxygen value displayed by the dissolved oxygen meter 9 in real time, and timely oxygenating and recovering when the dissolved oxygen value is lower than the oxygen concentration value required by the experiment.
Example 2:
the difference from the embodiment 1 is that: and a sealing ring 13 is arranged between the barrel cover 11 and the barrel body 1. The sealing ring 13 further improves the sealing effect between the barrel cover 11 and the barrel body 1.
A cushion block 5 is arranged at the bottom of the support frame 4; the bottom of cushion 5 is equipped with the rubber pad. The bottom of support frame 4 be equipped with cushion 5, the bottom of cushion 5 is equipped with the rubber pad, is in order to make support frame 4's support nature better, be difficult for taking place to slide in the staving.
The method of use of this example is the same as example 1.
Example 3:
the difference from the embodiment 1 is that:
the top of the sealing cover 15 is hinged with the top of the exhaust funnel 14. The top of the sealing cover 15 is hinged with the top of the exhaust funnel 14, so that the sealing cover 15 is convenient to open and close.
The middle of the top of the barrel cover 11 is provided with a handle 12.
The barrel body 1 is made of transparent acrylic or glass. The operation condition of the device in the barrel and the death condition of the oysters can be observed in the experimental process.
The method of use of this example is the same as example 1.
Example 4:
the difference from the embodiment 1 is that:
and a sealing ring 13 is arranged between the barrel cover 11 and the barrel body 1.
A cushion block 5 is arranged at the bottom of the support frame 4; the bottom of cushion 5 is equipped with the rubber pad.
The top of the sealing cover 15 is hinged with the top of the exhaust funnel 14.
The middle of the top of the barrel cover 11 is provided with a handle 12.
The barrel body 1 is made of transparent acrylic or glass. The operation condition of the device in the barrel and the death condition of the oysters can be observed in the experimental process.
The method of use of this example is the same as example 1.
It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The foregoing are merely exemplary embodiments of the present invention, which enable those skilled in the art to understand or practice the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the utility model. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (6)

1. The utility model provides an oyster hypoxemia stress experimental apparatus which characterized in that: comprises a barrel body (1), a filter (2), a support frame (4), an air pump (7), an oxygen dissolving instrument (9), a wave making pump (10), a barrel cover (11), and a nitrogen tank (16) and an oxygen tank (17) which are arranged on one side of the barrel body (1);
one side of the bottom end in the barrel body (1) is respectively provided with a bubble bar (6) and a heating rod (8), and the other side is provided with a filter (2); the nitrogen tank (16) and the oxygen tank (17) are respectively connected with the air inlet of the air pump (7) through a conduit, and the air outlet of the air pump (7) is connected with the air inlet of the bubble bar (6); a flow regulating valve A is arranged on a conduit connected with the nitrogen tank (16), and a flow regulating valve B is arranged on a conduit connected with the oxygen tank (17);
the middle part of the barrel body (1) is provided with an oxygen dissolving instrument (9), and the detection end of the oxygen dissolving instrument (9) extends into the barrel body (1);
a wave making pump (10) is arranged on one side of the upper part of the barrel body (1), and an exhaust funnel (14) is arranged on the other side of the upper part of the barrel body (1); a sealing cover (15) is arranged at the outlet of the exhaust funnel (14);
a support frame (4) is arranged in the middle of the interior of the barrel body (1); a tray body (3) is arranged between the support frames (4); holes are uniformly distributed in the middle of the tray body (3);
the top opening of the barrel body (1) is connected with a barrel cover (11).
2. The oyster hypoxia stress experimental facility of claim 1, wherein: and a sealing ring (13) is arranged between the barrel cover (11) and the barrel body (1).
3. The oyster hypoxia stress experimental facility of claim 1, wherein: the bottom of support frame (4) be equipped with cushion (5), the bottom of cushion (5) is equipped with the rubber pad.
4. The oyster hypoxia stress experimental facility of claim 1, wherein: the top of the sealing cover (15) is hinged with the top of the exhaust funnel (14).
5. The oyster hypoxia stress experimental facility of claim 1, wherein: the middle of the top of the barrel cover (11) is provided with a handle (12).
6. The oyster hypoxia stress experimental facility of claim 1, wherein: the barrel body (1) is made of transparent acrylic or glass.
CN202120549104.3U 2021-03-17 2021-03-17 Oyster hypoxemia stress experimental apparatus Active CN215346520U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202120549104.3U CN215346520U (en) 2021-03-17 2021-03-17 Oyster hypoxemia stress experimental apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202120549104.3U CN215346520U (en) 2021-03-17 2021-03-17 Oyster hypoxemia stress experimental apparatus

Publications (1)

Publication Number Publication Date
CN215346520U true CN215346520U (en) 2021-12-31

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ID=79624626

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202120549104.3U Active CN215346520U (en) 2021-03-17 2021-03-17 Oyster hypoxemia stress experimental apparatus

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CN (1) CN215346520U (en)

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