KR101844898B1 - High-density mass cultivation apparatus for phytoplankton - Google Patents

Abstract

A high-density mass cultivation apparatus for phytoplankton according to the present invention comprises: a culture water tank having a predetermined volume and having an opening at an upper portion and a culture liquid discharge valve at one side of a lower portion; a gas supply pipe having one end connected to the lower side of the culture water tank and the other end connected to a gas supply device; a gas supply valve adjusting the supply of the gas provided through the gas supply pipe to the culture water tank; an illumination device formed on the upper side or the side of the culture water tank and supplying light into the culture water tank; and a frame capable of adjusting the position and angle of the illumination device by fixing the illumination device, thereby providing the high-density mass cultivation apparatus for phytoplankton. Therefore, it is possible to study not only the physiological activity at the cellular level of red tide species such as Cochlodinium, but also the interaction mechanism with fish, thereby being a great help to the study of red tide.

Description

[0001] HIGH-DENSITY MASS CULTIVATION APPARATUS FOR PHYTOPLANKTON [0002]

The present invention relates to a device for high-density mass culture of phytoplankton, and more particularly, to a phytoplankton high-density mass culture apparatus in which a plurality of gas supply devices are formed in a culture tank and a culture tank, .

Red tide is a phenomenon in which plankton is abnormally proliferated or biologically and physically integrated to discolor sea, rivers, canals and lakes, and adversely affect aquatic organisms. The occurrence of red tides causes problems in ecosystems, especially in marine ecosystems, and affects human life in many ways. The greatest cause of red tide is the eutrophication of water, that is, when there are too many organic nutrients in the water. In the past, phosphorus contained in soaps and detergents became a problem, but in recent years, besides the cause of nutrient supply, reduction of tidal flats due to coastal development has become a big problem.

Many living creatures in the tidal flats have played a role of natural remediation to maintain these levels to some extent by feeding microorganisms and plankton in the water. However, due to such activities as reclamation, tidal flats decrease and eutrophication becomes more severe, . It is also known that red tide occurs when the temperature of the water increases due to the change of temperature and the microorganisms reproduce more vigorously or when the wind is not blowing so much that the sea water does not mix well. Especially, it is known that red tide appears more frequently due to the increase of water temperature due to changes in the global environment such as El Niño.

When red tide occurs, the concentration of oxygen dissolved in the water is lowered, so that the fish and shellfish breathing using oxygen in the water suffocate and die. In addition, there are some cases where plankton is physically suffocated by fish gills, and there are some algae that are toxic among the plankton causing red tides, which are dead due to this toxicity. Because of this, when red tide occurs, it may not only hurt fishery, especially aquaculture, but it may also cause symptoms of poisoning by ingesting fish and shellfish that accumulate toxic substances.

Red tides generally appear differently depending on the color of plankton, and may also be orange, reddish brown, brown, etc. This is because creatures causing red tides have carotenoids like red and brown pigments in addition to chlorophyll. Phytoplankton such as diatom and dinoflagellate are the most common plankton causing red tides, and red tide standards in Korea also use the amounts of these two plankton. In addition, cyanobacteria (cyanobacteria), prototypes (noctiluca), and protozoan (mesodinium) may occur in the red tide.

In Korea, cochlodinium polykrikoides red tide, which causes large-scale fisheries damage, started to occur in 1990, and it has a tendency to occur for a long time in a wide area. In Korea, the farms are concentrated in the inland waters near the shoreline, so that it is easy to inflow the red tide farms, which causes a great deal of damage when red tide occurs. Especially, since 1995, about 76.4 billion won of massive fisheries damage have been caused, there has been a yearly red tide almost every year, causing fisheries damage of more than several hundred million won.

In order to minimize the damage caused by red tide, the physiology and ecology of red tide creatures, research on the mechanism of fish death, and development of various materials and equipment for red tide creatures should be continued throughout the year. For continuous research, high-density red tide organisms must be supplied throughout the year, and in particular, high-density red tide organisms are required to carry out large-scale red tide organ cultivation in order to conduct research on red tide for large-scale organisms such as fish.

However, with the present technology, it is difficult to cultivate high density of species such as cochlearnidium. Therefore, the research is being conducted for a limited period of time by collecting the red tide occurring at the site during the summer tide occurrence period. High-density mass cultivation of these red tide organisms is not secured at home and abroad. Therefore, it is urgent to develop a culture apparatus as a part of securing red tide biological high-density technology.

Korean Patent Registration No. 10-0500651 discloses a first aspect of the present invention relates to a multi-stage prototype culture vessel which is sequentially connected to a connection pipe capable of opening and closing; And a plurality of biological culture vessels, each of which is connected to an openable and closable connection channel in sequence. The biological culture vessel for causing red tide is connected to a cultivating vessel for raw biological material predominant at the bottom and a connection channel for opening and closing, And a large-scale culture apparatus for predator-causing organisms and an organism-causing predator including a physical culture predator mass culture apparatus. In Korean Patent No. 10-0903691, a raw water tank for storing raw water supplied from a fresh water lake; The filtration unit separating the phytoplankton and the zooplankton contained in the raw water discharged from the raw water tank and the at least one first culture tank for containing and culturing the phytoplankton filtered at the filtration unit and the unfiltered zooplankton are accommodated in the filtration unit, And at least one second culture tank connected to receive the phytoplankton cultured in the first culture tank, wherein the zooplankton cultivated in the second culture tank is discharged into fresh water, And a predatory culture device capable of predation. Korean Patent Registration No. 10-1290810 discloses a culture tank for culturing microalgae using effluent in which carbon dioxide is dissolved as a culture water and a useful substance accumulation tank for inducing the accumulation of useful substances in microalgae cultured through the culture tank Wherein the useful substance accumulation tank is divided into at least two zones and is provided in the form of a container having a constant width along the periphery of the culture tank, wherein an inner space is used as a culture tank, and an opening / Discloses a microalgae culture system in which microalgae cultured from a culture tank are configured to be directly input into a useful substance accumulation tank divided into respective zones. Korean Patent Laid-Open Publication No. 10-2012-0095826 discloses a microalgae culture reactor having a closed structure and a culture solution outlet formed at a predetermined position at the lower end thereof; A reactor cap detachably coupled to the top of the microalgae culture reactor; A culture fluid inlet pipe and an air discharge pipe inserted into the reactor stopper so as to communicate with the inside of the microalgae culture reactor; An air injection unit detachably coupled to a lower end of the microalgae culture reactor to supply air or combustion gas into the microalgae culture reactor; The present invention relates to a microbiological culture bioreactor for microalgae culture, and a microalgae culture and harvesting method using the same. However, the above-mentioned inventions are characterized in that a plurality of branches are formed under the culture tank under the culture tank capable of culturing phytoplankton, and the gas is supplied at a time difference in the culture liquid. Respectively.

Conventional optical incubators or photoreactors can be used for culture of phytoplankton cultivated in a variety of apparatuses such as a culture tank, a light condition, a temperature condition, a gas supply into a culture medium, There was a problem of large scale and complexity. Accordingly, the present invention relates to a method for rapidly studying phytoplankton including red tide organisms, a method for rapidly culturing large quantities of the phytoplankton in a laboratory, phytoplankton stagnation inside the incubator, removal of growth inhibitory gases such as ammonia, The purpose of the device is to provide.

In order to solve the above problems, the present invention is characterized in that a cylindrical culture water tank having a predetermined volume is formed, and a plurality of gas injection pipes are formed on the lower side of the culture water tank. A cylindrical culture water tank having a predetermined volume is formed, And a gas generator in which a plurality of gas injection pipes are formed on the side surface and connected to the open top of the gas injection pipe by a silicone tube.
The present invention is characterized in that a cylindrical culture water tank having a predetermined volume is formed and a plurality of gas injection pipes are formed on the lower side of the culture water tank and a gas generator connected to the open top of the gas injection pipe by a silicone tube, The silicon tube of the present invention may be a high-density phytoplankton mass culture apparatus in which a filter is formed to remove foreign matter.
In the embodiment of the present invention, a phytoplankton high-density mass culture apparatus has a culture tank having a predetermined volume and an opening formed at an upper portion thereof and a culture liquid discharge valve formed at a lower portion thereof, one end connected to a lower side of the culture water tank, A gas supply valve connected to the apparatus, a gas supply valve for regulating the supply of the gas supplied through the gas supply pipe to the culture water tank, an illumination device formed on the upper or side of the culture water tank and supplying light into the culture water tank, And a frame which is fixed and adjusts the position and the angle of the lighting device. The present invention also provides a high-density phytoplankton mass culture apparatus.

A large amount of phytoplankton containing red tide organisms can be cultivated at high speed in a laboratory for the study of phytoplankton, especially red tide organisms, by using the phytoplankton high density culture apparatus according to the present invention. It is possible to induce agitation of the cultured organism and prevent the cultured organism from stagnating or stopping in the incubator.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a perspective view of a high density, mass culture apparatus for phytoplankton according to the present invention.
2 is a front view of a high density bulk phytoplankton culture apparatus according to the present invention.
3 is a front view schematically showing the configuration of a high-density mass culture apparatus for phytoplankton according to the present invention.
4 is a view showing a culture tank of a phytoplankton high-density mass culture apparatus according to the present invention.
FIG. 5 is a view showing a circulation plate of a high-density mass culture apparatus for phytoplankton according to the present invention.
FIG. 6 is a front view of a high density bulk culture apparatus for phytoplankton according to the present invention.
7 is a photograph of a high density bulk phytoplankton culture apparatus according to the present invention.
8 is a side view of a high density bulk phytoplankton culture apparatus according to the present invention.
FIG. 9 is a front view of a high density bulk phytoplankton culture apparatus according to the present invention.
Figure 10 is a photograph of the air supply valve of a high density, mass culture apparatus for phytoplankton according to the present invention.
11 is a photograph of a culture liquid discharge valve of a phytoplankton high density bulk culture apparatus according to the present invention.
FIG. 12 is a front view of a lighting apparatus of a high-density mass culture apparatus for phytoplankton according to the present invention.
FIG. 13 is a side view of a lighting apparatus of a high-density mass culture apparatus for phytoplankton according to the present invention.
FIG. 14 is a photograph of a metal halide and a fluorescent lamp, which are illumination lights of a high-density mass culture apparatus for phytoplankton according to the present invention.
FIG. 15 is a photograph showing a height adjustment device of a lighting apparatus of a high-density mass culture apparatus for phytoplankton according to the present invention.
FIG. 16 is a photograph showing a large-scale culture of cochlodinium using a phytoplankton high-density mass culture apparatus according to the present invention.

The high density culture apparatus for phytoplankton according to the present invention is designed to cultivate phytoplankton, especially phytoplankton causing red tide, at a high speed in a laboratory. Hereinafter, the present invention will be described in detail with reference to specific examples.

FIG. 1 is a perspective view of a high-density phytoplankton mass culture apparatus according to the present invention, FIG. 2 is a front view thereof, and FIG. 3 is a front view schematically illustrating the configuration of a high-density phytoplankton mass culture apparatus according to the present invention. The apparatus for mass culturing phytoplankton according to the present invention comprises a culture water tank 100 having a predetermined volume and an opening formed at an upper portion thereof and a culture liquid discharge valve formed at a lower side thereof, one end of the culture water tank 100 is connected to a lower side of the culture water tank 100 And a gas supply valve 210 for regulating the supply of the gas provided through the gas supply pipe 200 to the culture water tank. The gas supply valve 210, which is formed on the upper or the side of the culture water tank, And a frame 310 for fixing and fixing the position and the angle of the illuminating device to the illuminating device 300 to provide a high density dense large-scale phytoplankton culture device.

The culture water tank 100 accommodates a culture solution containing phytoplankton containing the creatures causing red tide. The culture water tank 100 is preferably made of a transparent or semitransparent material so that photosynthetic action can be smoothly performed to grow phytoplankton at a high density, so that light can be easily transmitted through the culture liquid in the culture container. The culture water tank 100 can be made of transparent or semitransparent glass, pyrex, Pyrex material which has good light transmittance and is resistant to physical impact, transparent acrylic which facilitates mass culture and has a low risk of breakage desirable.

In addition, since the environment of the ocean is not stagnant and the sea water is always moving, air bubbles are injected so that the culture liquid in the culture tank (100) is always stagnant so that the phytoplankton can grow in an environment similar to nature. . To this end, the culture water tank 100 is formed in a cylindrical shape so that the water flow can be easily moved through the lower arcuate bottom. In the upper part of the culture water tank 100, an opening 101 is formed so that the gas supplied into the culture water tank 100 dissolves in the culture liquid and a part of the gas is allowed to escape to the upper part to facilitate gas communication and the culture liquid or phytoplankton To be performed easily. A transparent lid can be formed in the opening 101 to open and close.

A culture liquid discharge valve 102 is formed below the culture water tank 100 to facilitate the recovery of phytoplankton cultured in the culture water tank 100. And can be easily used for washing the culture water tank 100 and the like.

4 is a view showing a culture tank of a phytoplankton high-density mass culture apparatus according to the present invention. A gas supply valve 210 for supplying a gas to the culture water tank is formed at a lower portion of the culture water tank 100, and a filtration filter, a timer, and the like may be formed. At this time, a plurality of gas supply valves 210 are regularly formed in the lower part of the culture water tank 100 in the left, right, front and back directions.

The plurality of gas supply valves 210 may be connected to a timer and controlled by a timer. At this time, the gas supply valve connected to the culture water tank 100 may be connected to the gas supply valve 210 are alternately opened and closed so that the culture liquid in the culture water tank 100 is instantly stirred by the air bubbles to which the culture liquid is supplied. The supplied gas may be compressed carbon dioxide, and when the air compressor is used, the power supply unit may be connected to the frame 310 and used.

In addition, in order to mass-grow phytoplankton, light must be supplied for photosynthesis necessary for growth of phytoplankton. In order to cultivate a large amount of coccodinium species, which is one of the phytoplankton, especially the red plankton, in the present invention, even if phytoplankton forms a high density in the culture water tank 100, the light for photosynthesis can reach the inside of the culture water tank Respectively. For this purpose, the light source used in the illumination device 300 of the present invention may be a metal halide, a fluorescent lamp, an LED, or the like.

FIG. 5 is a view showing a circulating plate installed inside a high-density mass culture apparatus for phytoplankton according to the present invention. A circulation plate 103 capable of inducing the breeding water circulation can be installed in the culture water tank 100 to circulate the water in the culture water tank 100 more efficiently and efficiently. The circulation plate 103 is provided on the lower portion of the culture water tank 100, and is formed in a curved plate shape disposed inside in parallel with the outer peripheral curved surface of the culture water tank 100. When the gas is supplied to the gas supply valve 210 connected to the lower portion of the culture water tank 100, the gas moves at a high speed along a narrow space formed between the circulation plate 103 and the bottom surface of the culture water tank 100 , The pressure between the circulating plate and the bottom plate of the culture tank decreases according to the Bernoulli theorem, and the surrounding water, which is the normal pressure, moves into the low-pressure space and collects the collected water, And it is possible to circulate the breeding water by a flow of 5 to 10 times compared to the stopping time. In addition, since the gas is sequentially supplied by the gas supply valve 210 formed symmetrically to the right and left of the culture water tank 100, the circulation of the water in the culture water tank 100 can be maximized.

The circulation plate 103 is spaced apart from the bottom surface of the culture water tank 100 by a support 104 formed on the inner lower side of the culture water tank 100 and the support 104 is pillar- . The height of the column can be adjusted to be adjustable in a multistage manner, so that the flow rate of the breeding water occurring between the circulating plate and the bottom plate of the culture tank can be adjusted according to the height adjustment.

FIG. 6 is a front view of a phytoplankton high-density mass culture apparatus according to the present invention, FIG. 7 is an oblique view, and FIG. 8 is a side view. A water tank 110 can be formed at a lower portion of the culture water tank 100 so as to stably support the culture water tank 100 and the culture liquid contained therein. In addition, a moving wheel is formed in the water tank 100 and a phytoplankton high- Can be easily moved.

FIG. 9 is a front view of a culture tank of a phytoplankton high-density mass culture apparatus according to the present invention, FIG. 10 is a photograph of an air supply valve, and FIG. 11 is a photograph of a culture liquid discharge valve. The water tank 100 may be provided with grooves for stably accommodating the culture water tank 100 and the gas supply pipe 200. The water tray 110 can separate the culture water tank 100 from the floor at a predetermined height to provide a space in which the culture liquid discharge valve 102 is formed to facilitate recovery of the culture liquid. A water tank fixing device may be further formed at a lower portion of the culture water tank 100, where the water tank support 110 meets.

FIG. 12 is a front view of a lighting device of a phytoplankton high-density mass culture apparatus according to the present invention, which is actually manufactured, and FIG. 13 is a side view thereof.

In order to continuously study to minimize fisheries damage caused by red tide, high-density red tide organisms must be supplied throughout the year. Especially, in order to carry out research on red tide for large-sized organisms such as fish, However, in the present technology, it is difficult to cultivate high-density cultivars such as cochlodinum which cause red tide, and research is ongoing for a limited period of time by collecting red tide from the site during the summer tide occurrence period. In order to solve this problem, in order to solve this problem, a large amount of phytoplankton is continuously stirred by the sequential aeration of bubbles, but cultured without the stress that the phytoplankton is destroyed by the bottom of the cylindrical culture tank.

FIG. 14 is a photograph of a metal halide and a fluorescent lamp used as an illumination light of a phytoplankton high-density mass culture apparatus according to the present invention, and FIG. 15 is a photograph showing an apparatus for controlling height of a lighting apparatus of a phytoplankton high-density mass culture apparatus. For effective photosynthesis, it is possible to freely move the position, height, and angle of the lighting device according to the culture state and density of the phytoplankton, and it is confirmed that high-density culture of the causative organism of red tide origin is possible by uniformly irradiating the phytoplankton . In particular, the metal halide lamp has an optimal wavelength for light synthesis, strong brightness, and excellent color rendering property, which is preferable for high density large-scale cultivation of phytoplankton.

FIG. 16 is a photograph showing a large-scale culture of cochlodinium using a phytoplankton high-density mass culture apparatus according to the present invention. By using a high-density mass culture apparatus of phytoplankton of the present invention, it is possible to study the physiological activity at the cellular level of the red tide species as well as the interaction mechanism with the fish by high-density mass culture of the causative organisms causing red tide such as cochlearnidium This can be helpful in red tide studies.

In addition, the high density culture apparatus for phytoplankton according to the present invention can be applied not only to red tide creatures but also to high density cultivation of phytoplankton such as phytoplankton which is capable of high density culture of phytoplankton, which is a seaweed bivalve living organism, and various physiologically active substances.

The present invention provides a high-density mass culture apparatus for phytoplankton which can be mass-cultured at a high speed in a laboratory for the study of phytoplankton, especially red tide creatures. Therefore, the present invention is useful for the study of red tide and the utilization industry of phytoplankton, .

100: culture tank 101: culture tank opening
102: Culture liquid discharge valve 103: Circulation plate
104: Support body 110: Water tray support
200: gas supply pipe 210: gas supply valve
300: illumination device 310: frame

Claims (6)

A culture water tank having a predetermined volume and having an opening at an upper portion thereof and a culture liquid discharge valve at a lower portion thereof;
A circulation plate which is located inside the culture water tank and is formed as a curved surface disposed in parallel to the outer peripheral curved surface of the culture water tank to maximize circulation of feed water by gas supply; Wherein the circulation plate is fixedly formed on the inner bottom surface of the culture water tank by a multi-stage adjustable support,
A plurality of gas supply pipes having one end connected to the lower side of the culture water tank and the other end connected to the gas supply device; And a gas supply valve for regulating the supply of the gas provided through each of the gas supply pipes to the culture water tank is formed, and one side of the gas supply valve is connected to a timer to control opening and closing, So that the gas is supplied sequentially or at a time interval between the circulating plate of the culture tank and the space formed by the inner bottom of the culture tank;
An illumination device formed on the upper side or the side of the culture water tank and supplying light into the culture water tank; And a frame capable of adjusting the position and angle of the illuminating device by fixing the illuminating device to the high-density phytoplankton. delete delete delete delete delete

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