CN217470937U - Continuous algae culture device - Google Patents
Continuous algae culture device Download PDFInfo
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- CN217470937U CN217470937U CN202220594906.0U CN202220594906U CN217470937U CN 217470937 U CN217470937 U CN 217470937U CN 202220594906 U CN202220594906 U CN 202220594906U CN 217470937 U CN217470937 U CN 217470937U
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Abstract
The utility model discloses an algae continuous culture device, which comprises a light culture device and a dark culture device, wherein the light culture device and the dark culture device are horizontally arranged or arranged in a staggered way, the light culture device is connected with a culture solution input device through a first pipeline, the outlet of the first pipeline is arranged at the upper end inside the light culture device, the light culture device is connected with the dark culture device through a second pipeline, the inlet of the second pipeline is arranged at the bottom of the light culture device, the outlet is arranged at the upper end inside the dark culture device, the dark culture device is connected with an algae product processing device through a third pipeline, the inlet of the third pipeline is arranged at the bottom of the dark culture device, the dark culture device is connected with the light culture device through a return pipeline, at least one of the second pipeline and the return pipeline is provided with a liquid conveying pump, the bottoms of the light incubator and the dark incubator are provided with a waste material discharge device, and the light incubator is also provided with a light source. And a continuous culture mode is adopted, so that the utilization rate of equipment is improved, and the industrial continuous production is facilitated.
Description
Technical Field
The utility model relates to an alga culture plate technical field, concretely relates to alga continuous culture plate.
Background
Algae are lower plants without true root, stem, leaf differentiation, have chloroplasts, perform photoautotrophic action, and are distributed on land, water or even float in the atmosphere. In nature, the algae exist in the forms of single cells, colony, filament, heterofilament (the algae filament is composed of erect branch and creeping branch), polykaryon, pseudoparenchyma and parenchyma, and the body sizes are different from several microns to tens of meters. Although the algae is tiny, the algae has very high material exchange efficiency with the external environment, can quickly absorb nutrition, efficiently utilize light energy, and has the characteristics of high growth speed and high photosynthetic efficiency. Many algae are rich in nutrition, are sources of ideal food and health care products, and some micro algae are natural starter baits of fish, shrimps, crabs and shellfish and are also important in aquaculture breeding and cultivation, so the algae have higher development and utilization values.
In recent years, more and more research has focused on the cultivation of algae, which have relatively simple nutritional requirements and need only provide the necessary moisture, air, light and the necessary inorganic salts. The culture of algae is mainly divided into 3 types of one-time culture, semi-continuous culture and continuous culture according to a harvesting mode, wherein the continuous culture refers to that algae cells are continuously harvested and nutrient salts are added according to the density of algae or the consumption of the nutrient salts in a culture solution after inoculation. According to the growth characteristics of the algae cells, the growth of the algae cells mainly comprises four stages of a slow phase, a logarithmic phase, a stationary phase and a decay phase, the algae cells in the logarithmic phase grow and propagate fast, and related products of algae can be obtained more easily.
In the prior art, when algae cells are cultured in a continuous culture mode, a culture solution is generally input into a culture device by a constant flow pump or an automatic input pump, gas required by the growth of algae is introduced into the culture device by a vent pipe, and the algae is prevented from agglomerating by stirring by a magnetic stirrer in the culture process. However, according to the growth habit of algae, the cultivation of algae requires alternation of light and dark, and the growth of algae is rather inhibited by cultivation only under light conditions without closed light cultivation. On the other hand, the nutrients required by the algae in the light and dark conditions are different greatly, for example, the algae needs a large amount of carbon dioxide for photosynthesis under the light condition, while the algae needs respiration under the dark condition, and the extra increase of the oxygen content is beneficial to the better growth of the algae. Therefore, the development of an algae cultivation apparatus having both a light incubator and a dark incubator is a necessary trend to improve the yield of algae.
Disclosure of Invention
An object of the utility model is to overcome the defect that exists among the prior art, the utility model provides an algae continuous culture ware is provided, this culture ware contains light culture ware and dark culture ware, the entrance of light culture ware links to each other with culture solution input device, the export is continuous with dark culture ware through first transfer pump, the exit of dark culture ware is connected with the second transfer pump, the other end of second transfer pump links to each other with algae product processing apparatus, all be provided with filter equipment in light culture ware and the dark culture ware, a detection device, a stirring apparatus, heating device and air inlet and exhaust apparatus, still be provided with the light source in the light culture ware. By dividing the incubator into a light incubator and a dark incubator and arranging corresponding auxiliary devices in the light incubator and the dark incubator respectively, favorable conditions are provided for photosynthesis of algae in the light incubator and respiration in the dark incubator. When the incubator of the utility model is used for algae cultivation, the algae has more favorable growth conditions and higher productivity.
In order to achieve the above object, the present invention provides an algae continuous culture apparatus, comprising a light culture apparatus and a dark culture apparatus, wherein the light culture apparatus and the dark culture apparatus are horizontally arranged or staggered, the light culture apparatus is connected to a culture solution input device through a first pipeline, an outlet of the first pipeline is arranged at an upper end inside the light culture apparatus, the light culture apparatus and the dark culture apparatus are connected through a second pipeline, an inlet of the second pipeline is arranged at a bottom of the light culture apparatus, an outlet of the second pipeline is arranged at an upper end inside the dark culture apparatus, the dark culture apparatus is connected to an algae product processing device through a third pipeline, an inlet of the third pipeline is arranged at a bottom of the dark culture apparatus, the dark culture apparatus is connected to the light culture apparatus through a return pipeline, the second pipeline and the return pipeline are connected, at least one pipeline is provided with an infusion pump, the bottoms of the light incubator and the dark incubator are provided with waste material discharge devices, and a light source is also arranged in the light incubator.
A first infusion pump is arranged on the second pipeline, a second infusion pump is arranged on the third pipeline, the second infusion pump is connected with the light incubator through a return pipeline, a first waste discharge pipeline and a second waste discharge pipeline are respectively arranged at the bottoms of the light incubator and the dark incubator, and a first stop valve, a second stop valve, a third stop valve, a fourth stop valve, a fifth stop valve and a sixth stop valve are respectively arranged on the first pipeline, the second pipeline, the third pipeline, the return pipeline, the first waste discharge pipeline and the second waste discharge pipeline; and a filtering device, a detection device, a stirring device, a heating device, an air inlet device and an air exhaust device are arranged in the light incubator and the dark incubator.
When the algae cell culture is actually carried out, a pump in the culture solution input device conveys the culture solution to the light incubator through a first pipeline, after the algae cells are cultured in the light incubator for a period of time, the culture solution flows into the dark incubator through a second pipeline for culture, algae products cultured in the dark incubator are discharged into the algae product treatment device through a third pipeline for further treatment, and the culture solution which does not grow into algae finished products flows back into the light incubator for continuous culture.
In a further technical scheme, when algae are cultured in the light incubator, the light source provides illumination conditions for photosynthesis of algae cells, and CO 2 Is delivered into the light incubator through the air inlet device to carry out the culture of the algae cellsConditions are provided for photosynthesis, the heating device can heat the optical incubator to provide proper temperature for growth of algae cells, and the detection device can detect pH, temperature, algae density and CO of the culture solution 2 The concentration provides the most suitable condition for the growth of the algae cells. The stirring device keeps the stirring state all the time, prevents the algal cells from agglomerating, and improves the growth efficiency of the algal cells. The algae cells inevitably generate a certain amount of metabolic waste in the growth process, and the filtering device has the main function of discharging the metabolic waste, so that the algae cells have a better growth environment and can keep a higher growth rate. After algae cells are cultured in a light incubator for a period of time, culture solution in the light incubator is conveyed to a dark incubator through a second pipeline under the action of a first liquid conveying pump, and O 2 Enters from an air inlet device in the dark incubator to provide necessary O for the algae cells to perform respiration 2 . After the algae cells are cultured in the dark incubator for a period of time, part of algae products are generated, the second infusion pump can convey the algae products in the dark incubator into the algae product processing device through the third pipeline, and the algae product processing device can further process the algae products. The algae cell culture solution in the dark culture chamber is conveyed to the light culture chamber through the return pipeline under the action of the second liquid conveying pump, the culture solution input device replenishes part of the culture solution, and all the culture solution is cultured in the light culture device and the dark culture device in a new round.
Further preferred technical scheme does, filter equipment includes the pellicle, the bottom inboard of light culture ware and dark culture ware is provided with the stainless steel net, the pellicle passes through pellicle fixer fixed connection respectively on the shell lateral wall and the stainless steel net of light culture ware and dark culture ware. Adopt the pellicle as filter equipment, set up stainless steel net in the bottom of incubator to pass through the pellicle fixer and fix respectively at the shell lateral wall of incubator and stainless steel net with the pellicle. Algal cells can produce metabolic waste or product after cultivateing a period in the incubator, and the main advantage of using the pellicle as filter equipment lies in that the pellicle has the characteristics that the micromolecule can pass through and the macromolecule can not pass through, cultivates algal cells in the pellicle, can guarantee that nutrient substance can get into the pellicle, can make the poisonous and harmful product of micromolecule flow out again, guarantees algal cells's activity.
The detection device comprises a sensor, one end of the sensor is fixed on the upper covers of the light incubator and the dark incubator, and the other end of the sensor is provided with a pH probe, a temperature probe, an algae density probe and a gas concentration probe and extends to the bottoms of the light incubator and the dark incubator. The pH probe, the temperature probe, the algae density probe and the gas concentration probe are combined together to form a sensor, the sensor is used as a detection device, the sensor integrates pH detection, temperature detection, algae density detection and gas concentration detection devices, and space occupation caused by the arrangement of a plurality of detection devices in the incubator can be avoided.
According to a further preferred technical scheme, the stirring device comprises a stirrer, the stirrer comprises a motor and a stirring part connected to the motor, the motor is fixed on the upper covers of the light incubator and the dark incubator, and the stirring part extends to the bottoms of the light incubator and the dark incubator. The stirring device can also be a magnetic stirrer, the magnetic stirrer comprises a magnetic stirrer and a magnet, the magnetic stirrer is placed at the bottom in the light incubator and the dark incubator, and the magnet is arranged at the bottom outside the light incubator and the dark incubator. The main function of the stirrer is to stir the culture solution to prevent the algal cells in the culture solution from agglomerating during the growth process.
The heating device comprises a heating rod, one end of the heating rod is fixed on the upper covers of the light incubator and the dark incubator, and the other end of the heating rod extends towards the bottoms of the light incubator and the dark incubator. The heating rod is mainly arranged for heating the culture solution, providing proper temperature for the growth of the algae cells,
the technical scheme is that the light source is an underwater LED light source and comprises a fixing part and a light source part, the fixing part is arranged on the upper cover of the light incubator, the light source part extends to the bottom of the light incubator, a photocatalysis net is further arranged in the light incubator, and the light incubator is provided with a light sourceThe catalytic mesh is made by supporting a photocatalyst on polypropylene fibers. The main purpose of setting the light source is to provide necessary illumination conditions for photosynthesis of algae cells, and the light source is replaced by an underwater LED light source, so that the algae cell culture solution at the bottom of the light incubator can be ensured to contact with sufficient illumination, and necessary conditions are provided for growth of the algae cell culture solution. Preferably, a photocatalysis net is arranged in the light incubator and is formed by TiO 2 The photocatalyst is loaded on the polypropylene fiber net. The photocatalytic net is arranged inside the semi-permeable membrane in the optical incubator, is kept at a certain distance from the semi-permeable membrane, and can generate OH and O by the photocatalyst under the irradiation of an LED light source 2- Etc. active substances capable of decomposing toxic and harmful organic substances into CO 2 And H 2 And O, purifying the living environment of the algae cells. Although the high concentration of photocatalyst has the effect of inhibiting the growth of algal cells, the low concentration of specific catalyst has the effect of promoting the growth of algal cells. The powdered photocatalyst has the problems of difficult recovery, low repeated utilization rate and the like, and the photocatalyst is loaded on the polypropylene fiber, so that the activity of the photocatalyst can be ensured, and the photocatalyst can be conveniently recovered.
According to a further preferable technical scheme, the bottom of the light incubator and the bottom of the dark incubator are respectively provided with a nano aeration pipe, the nano aeration pipes are laid below the stainless steel mesh, a plurality of nano aeration holes are formed in the nano aeration pipes, and the upper covers of the light incubator and the dark incubator are respectively provided with an exhaust hole. This scheme adopts nanometer aeration pipe as air inlet unit to lay it in the below of light culture ware and dark culture ware bottom stainless steel net, be provided with nanometer aeration hole on the nanometer aeration pipe. Pumping CO into the nano aeration pipe in a light incubator 2 The necessary energy is provided for the photosynthesis of algae, and in a dark culture device, O needs to be pumped into the nano aeration pipe 2 The oxygen is provided for the algae cells to breathe, so that the activity of the algae cells is improved, and the growth of the algae is promoted.
The preferable technical scheme is that the number of the underwater LED light sources is a plurality, and the underwater LED light sources are uniformly arranged on the light incubator. The LED light sources which are arranged in a balanced manner are beneficial to the fact that algae cells at different positions in the optical incubator can receive illumination, and growth of the algae cells is promoted.
The utility model has the advantages and the beneficial effects that:
1. the culture device is divided into a light culture device and a dark culture device, corresponding conditions are provided for photosynthesis and respiration of algae cells respectively, and the retention time of the algae cells in the light culture device and the dark culture device is changed by adjusting the flow of each pipeline, so that the rapid growth of the algae cells is facilitated.
2. Culturing algae cells in a semi-permeable membrane, setting nano aeration below the semi-permeable membrane, and pumping CO into a light culture device and a dark culture device respectively 2 And O 2 The normal photosynthesis and respiration of the algae cells are guaranteed, the algae cells are kept at high activity, nutrient substances can be guaranteed to enter the semipermeable membrane through the arrangement of the semipermeable membrane, small molecular toxic and harmful products can flow out, and the high-speed growth of the algae cells is maintained.
3. The specific photocatalysis net is arranged in the optical incubator, can degrade metabolic wastes of algae cells and maintain the activity of the algae cells, and can generate a small amount of active substances, thereby being beneficial to promoting the growth of the algae.
Drawings
FIG. 1 is a schematic view of the structure of an incubator according to the embodiment of the present invention.
FIG. 2 is a plan view of the incubator according to the embodiment of the present invention.
FIG. 3 is a schematic diagram showing the arrangement of the nano-aeration tubes in the photo-incubator in the example.
In the figure: 1. a light incubator; 2. a dark incubator; 3. a first conduit; 4. a culture solution input device; 5. a second conduit; 6. a first infusion pump; 7. a third pipeline; 8. an algae product treatment device; 9. a second infusion pump; 10. a return line; 11. a first waste discharge conduit 11; 12. a second waste discharge conduit; 13. a first shut-off valve; 14. a second stop valve; 15. a third stop valve; 16. a fourth stop valve; 17. a fifth stop valve; 18. a sixth stop valve; 19. a stainless steel mesh; 20. a semi-permeable membrane; 21. a semipermeable membrane retainer; 22. a blender; 221. a motor; 222. a stirring section; 23. a sensor; 24. a heating rod; 25. an underwater LED light source; 26. a photocatalytic network; 27. a carbon dioxide nano aeration pipe; 28. an oxygen nano aeration pipe; 29. and (4) exhausting holes.
Detailed Description
The following description will further describe embodiments of the present invention with reference to the accompanying drawings and examples. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
Example 1
As shown in FIGS. 1 and 2, a continuous algae incubator comprises a light incubator 1 and a dark incubator 2, the light incubator 1 is connected to a culture medium feeding device 4 through a first pipe 3, an outlet of the first pipe 3 is provided at an upper end of an inside of the light incubator 1, the light incubator 1 is connected to the dark incubator 2 through a second pipe 5, an inlet of the second pipe 5 is provided at a bottom of the light incubator 1, an outlet is provided at an upper end of an inside of the dark incubator 2, a first liquid delivery pump 6 is provided on the second pipe 5, the dark incubator 2 is connected to an algae product treating device 8 through a third pipe 7, an inlet of the third pipe 7 is provided at a bottom of the dark incubator 2, a second liquid delivery pump 9 is provided on the third pipe 7, the second liquid delivery pump 9 is connected to the light incubator 1 through a return pipe 10, a first waste discharge pipe 11 and a second waste discharge pipe 12 are provided at bottoms of the light incubator 1 and the dark incubator 2, respectively, the first pipe 3, the second pipe 5, the third pipe 7, the return pipe 10, the first waste discharge pipe 11, and the second waste discharge pipe 12 are provided with a first stop valve 13, a second stop valve 14, a third stop valve 15, a fourth stop valve 16, a fifth stop valve 17, and a sixth stop valve 18, respectively. Stainless steel nets 19 are arranged on the inner sides of the bottoms of the light incubator 1 and the dark incubator 2, and the semipermeable membrane 20 is fixedly connected to the side walls of the shells of the light incubator 1 and the dark incubator 2 and the stainless steel nets 19 through semipermeable membrane fixing devices 21 respectively. The stirrer 22 is provided at the center of the light incubator 1 and the dark incubator 2, the stirrer 22 includes a motor 221 and a stirrer 222 connected to the motor 221, the motor 221 is fixed to the upper covers of the light incubator 1 and the dark incubator 2, and the stirrer 222 extends toward the bottoms of the light incubator 1 and the dark incubator 2. The light incubator 1 and the dark incubator 2 are also provided with a sensor 23 and a heating rod 24, one end of the sensor 23 and the heating rod 24 is fixed on the upper cover of the light incubator 1 and the dark incubator 2, and the other end extends to the bottom. The light incubator 1 is also provided with 4 underwater LED light sources 25, the 4 underwater LED light sources 25 are evenly arranged on the light incubator 1, one end of each LED light source 25 is fixed on the upper cover of the light incubator 1, and the other end of each LED light source 25 extends to the bottom. The photo incubator 1 is also provided with a photo catalytic net 26, and the photo catalytic net 26 is made by supporting a photo catalyst on polypropylene fibers. The bottom of the light incubator 1 and the bottom of the dark incubator 2 are respectively provided with a carbon dioxide nano aerator pipe 27 and an oxygen nano aerator pipe 28, the carbon dioxide nano aerator pipe 27 and the oxygen nano aerator pipe 28 are laid below the stainless steel net 19, the carbon dioxide nano aerator pipe 27 and the oxygen nano aerator pipe 28 are respectively provided with a plurality of nano aeration holes, and the upper covers of the light incubator 1 and the dark incubator 2 are respectively provided with an exhaust hole 29.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A continuous alga incubator is characterized by comprising a light incubator and a dark incubator, wherein the light incubator and the dark incubator are horizontally arranged or arranged in a staggered manner, the light incubator is connected with a culture solution input device through a first pipeline, an outlet of the first pipeline is arranged at the upper end inside the light incubator, the light incubator is connected with the dark incubator through a second pipeline, an inlet of the second pipeline is arranged at the bottom of the light incubator, an outlet of the second pipeline is arranged at the upper end inside the dark incubator, the dark incubator is connected with an alga product processing device through a third pipeline, an inlet of the third pipeline is arranged at the bottom of the dark incubator, the dark incubator is connected with the light incubator through a return pipeline, at least one pipeline of the second pipeline and the return pipeline is provided with a liquid conveying pump, and waste discharge devices are arranged at the bottoms of the light incubator and the dark incubator, the light incubator is also provided with a light source.
2. The continuous culture device according to claim 1, wherein the second pipeline is provided with a first infusion pump, the third pipeline is provided with a second infusion pump, the second infusion pump is connected with the light culture device through a return pipeline, the bottom of the light culture device and the dark culture device are respectively provided with a first waste discharge pipeline and a second waste discharge pipeline, and the first pipeline, the second pipeline, the third pipeline, the return pipeline and the first waste discharge pipeline and the second waste discharge pipeline are respectively provided with a first stop valve, a second stop valve, a third stop valve, a fourth stop valve, a fifth stop valve and a sixth stop valve; and a filtering device, a detection device, a stirring device, a heating device, an air inlet device and an air exhaust device are arranged in the light incubator and the dark incubator.
3. The continuous culture device according to claim 2, wherein the filter device comprises a semi-permeable membrane, the inner side of the bottom of the light culture device and the dark culture device is provided with a stainless steel net, and the semi-permeable membrane is fixedly connected on the side wall of the shell of the light culture device and the dark culture device and the stainless steel net through a semi-permeable membrane fixer respectively.
4. The continuous incubator according to claim 3, wherein the detection means comprises a sensor having one end fixed to the upper covers of the light incubator and the dark incubator and the other end provided with a probe and extending toward the bottoms of the light incubator and the dark incubator.
5. The continuous culture apparatus of claim 3, wherein the stirring device comprises a stirrer, the stirrer comprises a motor and a stirring part connected to the motor, the motor is fixed to the upper covers of the light culture apparatus and the dark culture apparatus, and the stirring part extends toward the bottoms of the light culture apparatus and the dark culture apparatus.
6. The continuous culture apparatus of claim 3, wherein the stirring device comprises a magnetic stirrer, the magnetic stirrer comprises a magnetic stirrer and a magnet, the magnetic stirrer is arranged at the inner bottom of the light culture apparatus and the dark culture apparatus, and the magnet is arranged at the outer bottom of the light culture apparatus and the dark culture apparatus.
7. The continuous incubator of claim 3, wherein the heating means comprises a heating rod having one end fixed to the upper covers of the light and dark incubators and the other end extending toward the bottoms of the light and dark incubators.
8. The continuous culture device according to any one of claims 4 to 7, wherein the light source is an underwater LED light source, and comprises a fixing part and a light source part, the fixing part is arranged on the upper cover of the light culture device, the light source part extends to the bottom of the light culture device, and a photocatalytic net is arranged in the light culture device, and the photocatalytic net is made by supporting a photocatalyst on polypropylene fibers.
9. The continuous culture device according to claim 8, wherein the bottom of the light culture device and the bottom of the dark culture device are respectively provided with a nano aeration pipe, the nano aeration pipe is laid below the stainless steel mesh, the nano aeration pipe is provided with a plurality of nano aeration holes, and the upper covers of the light culture device and the dark culture device are respectively provided with an exhaust hole.
10. The continuous incubator of claim 9, wherein the number of underwater LED light sources is several, and the underwater LED light sources are equally disposed on the light incubator.
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| CN202220594906.0U CN217470937U (en) | 2022-03-18 | 2022-03-18 | Continuous algae culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114431131A (en) * | 2022-01-07 | 2022-05-06 | 中国科学院东北地理与农业生态研究所 | Aeration culture method for improving survival rate of algae |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114431131A (en) * | 2022-01-07 | 2022-05-06 | 中国科学院东北地理与农业生态研究所 | Aeration culture method for improving survival rate of algae |
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