KR100903691B1 - Cultivation apparatus of living organism to cause retide and its predator - Google Patents

Abstract

An apparatus for cultivating protozoan predators preying red tide and green algae-causing microorganisms is provided to manufacture protozoan predators massively and with high purity. An apparatus for cultivating protozoan predators preying red tide and green algae-causing microorganisms includes a raw water tank(10), a filtering part(20), a plurality of first cultivation vessels(30), a plurality of second cultivation vessels(50), a discharge unit(80), and a constant temperature cultivation chamber(90). The raw water tank is supplied fresh water through a raw water supply route(1). A raw water supply pump(2) is installed in the supply route. The filtration part is installed on a connection pipe line(110) connecting the raw water tank, the first and the second cultivation vessels. The filtration part is installed on a diverging point(113) of a second connection pipe line(112). The second cultivation vessel is connected with the first cultivation vessel through a third cultivation vessel.

Description

Cultivation apparatus of living organism to cause retide and its predator

The present invention relates to a red tide and green algae causative organism and a protozoa predator culturing apparatus capable of predating them, and more particularly, to a device for incubating protozoa predators for these harmful organisms in large quantities with high purity. will be.

'Red-tide or Harmful algal bloom' is a phenomenon in which the plankton grows in the ocean and turns the search into red or brown, and microorganisms that occupy a part of the marine ecosystem are the main cause. These red tide-causing organisms (phytoplankton) cause photosynthesis on a principle similar to that of land plants, and increase their populations by dividing their bodies into two parts, which are good for natural light and nutrients in seawater alone. In Korea, various red tide creatures appear on each coast from spring to autumn, causing many material and economic damages.

Some species that cause red tide are trapped between the gill tissues of fish to dissolve the tissues, making it impossible to exchange oxygen, or penetrate into the gill membranes and destroy the capillaries, thereby reducing the function of the gills' gas exchange and Causes secondary infection. Lack of dissolved oxygen in water can also have fatal effects on sticking benthic organisms, leading to increased physiological stress, space competition, and increased predation.

In addition, frequent economic losses to marine animals and humans in relation to red tide are due to the toxic substances created by them. Toxins produced from harmful algae can cause temporary or permanent loss of fishery resources, impeding the development of fisheries activities and humanity. Threatens your health. In particular, the occurrence of red tide caused by toxic coarse algae is accompanied by the fatality of fish and invertebrates, which increase the mortality rate of marine invertebrates, stop their behavior, impede muscle control, reduce feeding rate and ejaculation rate, and heartbeat abnormalities. Causes such symptoms.

For the control of red tide, chemical red tide control method, marine bacteria use method, competitive exclusion method that minimizes the damage of red tide by using toxic red tide causative organisms and non-toxic organisms that have nutritional inhibitory relationship and zooplankton against phytoplankton The method using the predation principle of the is used.

Among the red tide control method, the chemical red tide control method may cause secondary pollution problems and environmental disturbances due to the use of chemicals, and there is a problem that a large amount of cost and time are consumed in mass production and use, transportation and handling.

The red tide control method by marine bacteria has a disadvantage that it is difficult to apply the appropriate marine life to each of the red tide being large-scaled, wide-area, high density, and toxic because many kinds of red tide causing organisms and complex conditions vary from species to species. In addition, the control method of red tide by competitive exclusion is time-consuming and costly in the isolation, identification and cultivation of non-toxic organisms in nutritional competition with the toxic red tide organisms, and there is a limit to the application of effective non-toxic organisms.

As a specific example of the method using the predation principle, Korean Patent Laid-Open Publication No. 98-025804 relates to a method of preventing and treating red tide using protozoal predators (animal plankton). Disclosed is a method of mass-producing a rapid protozoa predator and feeding it to a red tide region or a red tide predicted region to prey a red tide causative organism.

However, the red tide control method using the protozoa of the protozoa is difficult to grow the zooplankton and a lot of time, despite the advantage that the growth rate of the protozoa is very fast to effectively remove various types of red tide with a small amount of input. There is a problem that costs and expenses.

The present invention has been made in view of the above, and an object of the present invention is to provide a culture apparatus capable of producing a large amount of red algae and algae causative organisms and protozoa predators capable of predating them with high purity.

Red tide and green algae causative organisms of the present invention for achieving the above object and protozoa predator culture apparatus capable of feeding them comprises: a raw water tank for storing the raw water supplied from the freshwater lake; A filtering unit for separating phytoplankton and animal plankton contained in the raw water discharged from the raw water tank; At least one first culture tank for receiving and culturing phytoplankton filtered by the filtration unit; Animal phytoplankton that is not filtered in the filtration unit is accommodated and cultured, the at least one second culture tank connected to receive the phytoplankton cultured in the first culture tank; including, the zooplankton cultured in the second culture tank Is characterized in that discharged to the fresh water.

Here, the first culture tank, the phytoplankton can be accommodated and cultured therein, the top is open and the first culture tank body of transparent or translucent material; Insulating material installed on the outside of the first culture vessel body; And a stirrer for stirring the culture solution inside the first culture vessel body.

The stirrer may include a stirrer body rotatably installed in the first culture tank; It is preferable that the stirrer body is installed outside the first vessel brewing to provide a rotational force to rotate, the wind turbine is rotated by the wind.

In addition, the inner surface of the first culture vessel body is preferably provided with a light reflecting portion for reflecting light.

In addition, the second culture tank, the second culture tank body that can be cultured by receiving the zooplankton therein; A cover member installed to cover the upper portion of the second culture tank body; Insulating material installed on the outside of the second culture vessel body; And an agitator for agitating the culture solution inside the second culture vessel body using wind power.

The cover member may include: a light shielding film for shielding sunlight; It is preferable to include a; support frame installed on the upper portion of the second culture vessel body to support the light shielding film.

In addition, the raw water tank, raw water tank body; A heater installed inside the raw water tank body; And a temperature control unit for sensing the raw water temperature inside the raw water tank main body and selectively controlling the driving of the heater based on the sensed temperature to maintain the raw water in the raw water tank main body at a constant temperature.

In addition, the first and second culture tank, it is preferable to include a pond facility formed so as to artificially formed to a predetermined depth artificially on the land adjacent to the freshwater lake.

In addition, it is preferable to further include an incubation chamber provided to cultivate only high-purity zooplankton to be supplied to the second culture tank or to mass culture on site through the first and second culture tanks.

In addition, it is installed to be immersed in the freshwater lake, it is preferable to further include a discharge device for discharging the culture solution containing the zooplankton cultured in the second culture tank to a pond or freshwater lake of a predetermined depth in the lake.

In addition, the discharge device, the discharge pipe is installed to be located at 0.5 to 1m below the surface of the water, the discharge pipe for discharging the culture solution supplied to the second culture tank in a fresh water lake or reservoir; An anchor connected to the discharge pipe and fixed to the bottom; A buoy installed to be exposed on the water surface connected to the discharge pipe; And installed on the buoy warning lights for facility safety; preferably include.

According to the red tide causative organism of the present invention and the protozoa predator cultivation apparatus capable of predating them, an animal plankton capable of feeding phytoplankton (red tide causative organism) by drawing raw water of freshwater lake or reservoir where red tide or green algae occurred. Bio-predators) can be concentrated and supplied in large quantities to freshwater lakes or reservoirs.

Therefore, by reducing the concentration of phytoplankton in freshwater lakes or reservoirs, it is possible to prevent red tide or freshwater phenomena in freshwater lakes or reservoirs.

Hereinafter, with reference to the accompanying drawings will be described in detail the red tide or green algae cause organisms and protozoa predator culture apparatus capable of predating them.

Referring to Figure 1, the probiotic predator culture apparatus according to an embodiment of the present invention, the raw water tank 10 for storing the raw water supplied from the fresh water, and the red tide contained in the raw water discharged from the raw water tank 10 or Filtration unit 20 for separating green algae-causing organisms (hereinafter referred to as phytoplankton) and primitive botanical predators (hereinafter referred to as zooplankton), and a plurality of first to receive and culture phytoplankton filtered by the filtration unit 20. The culture tank 30, the some 2nd culture tank 50 which accommodates and cultures the zooplankton filtered by the filtration part 20, and the discharge apparatus 80 are provided.

The raw water tank 10 receives fresh water through the raw water supply path 1 and stores the fresh water. The raw water supply pump 2 is installed on the supply path 1 to forcibly pump fresh water to be filled in the raw water tank 10.

As shown in FIG. 2, the raw water tank 10 has a raw water tank main body 11, a heater 12 installed in the raw water tank main body 11, and raw water in the raw water tank main body 11. And a temperature control unit 13 for controlling to maintain the temperature. The temperature control unit 13 of the heater 12 on the basis of the temperature sensor 13a for detecting the raw water temperature in the raw water tank main body 11 and the temperature sensing information transmitted from the temperature sensor 13a. The heater control part 13b which controls drive is provided.

According to the above configuration, when the raw water in the raw water tank 10 is below the set temperature by detecting the temperature sensing information detected by the temperature sensor 13a in real time, the heater control unit 13b drives the heater 12 to control the raw water The raw water in the tank main body 11 is set to set temperature (20 degreeC-25 degreeC).

In addition, the water level sensor 14 is installed in the raw water tank main body 11, the raw water by controlling the driving of the raw water supply pump (2) based on the water level information transmitted from the water level detection sensor (14). It is further provided with a pump drive control unit 15 for maintaining a predetermined water level or more. And the raw water tank main body 11 is provided with an opening 17 for flowing the raw water overflowed to the outside. Here, a fine screen box (not shown) is installed at the inlet of the supply pump 2 to prevent the inflow of impurities and small organisms, and has a double filter structure inside and outside.

The filtration unit 20 is installed on the connection pipe 110 for connecting the raw water tank 10, the first culture tank 30 and the second culture tank (50). The filtration unit 20 is preferably installed at a branch point 113 of the first connection pipe 111 facing the first culture tank 30 and the second connection pipe 112 facing the second culture tank 50. It is good to be. The filtration unit 20 may have a filter structure of a predetermined size so that phytoplankton passes and animal plankton does not pass. Preferably, the filtration unit 20 may have a filter structure of 20 μm or 80 μm.

Therefore, the zooplankton contained in the raw water discharged from the raw water tank 10 is moved to the second culture tank 50, the phytoplankton is moved to the first culture tank 30 is accommodated.

The first culture vessel 30 is provided with a plurality. The first culture tank 30 is for culturing a large amount of phytoplankton, as shown in Figure 3, the first culture vessel body 31, which is installed outside the first culture vessel body 31 The heat insulating material 32 and the stirrer 33 which stir the culture liquid in the inside of the 1st culture tank main body 31 are provided.

The first culture vessel body 31 is open to the upper to accommodate the phytoplankton cultured therein is formed of a transparent or translucent material. The heat insulating material 32 is formed of a material such as urethane, styrofoam, is installed to surround the outside of the first culture vessel body 31, it is possible to insulate the culture medium inside the first culture vessel body 31. . The first culture vessel body 31 is transparent or translucent so that sunlight is introduced into the first culture vessel body 31 in a large amount so that phytoplankton can be efficiently cultured.

The stirrer 33 is a stirrer body 33a rotatably installed in the first culture vessel body 31 and a wind turbine 33b connected to the stirrer body 33a and rotating by wind power to provide rotational force. ). The wind turbine (33b) is rotatably installed on the outside of the first culture vessel body 31 to be rotated by the wind, may be connected coaxially with the stirrer body (33a), between the reducer It can also be installed and connected. As such, since the agitator body 33a may be driven by using the wind turbine 33b that generates power by the wind, there is an advantage in that maintenance costs can be reduced.

In addition, preferably, the inner surface of the first culture vessel body 31 is further provided with a reflector 36 for reflecting sunlight to allow the sunlight to stay inside the first culture vessel body 31 for a long time. good. The reflector 36 may be provided by coating an inner surface of the first culture vessel body 31, or may be provided by installing a reflective member.

The second culture tank 50 is connected to the second connection pipe 112 receives the raw water containing the zooplankton, and is connected to the first culture tank 30 through the third connection pipe 114 to the animal It is connected to receive a culture solution containing phytoplankton which is a food of plankton. That is, the zooplankton of the second culture tank 50 is supplied with the high purity phytoplankton cultivated in the first culture tank 30 through the third connection pipe 114 to be intensively cultured. do. As shown in FIG. 4, the second culture vessel 50 includes a second culture vessel body 51, a heat insulating material 52, a cover member 53, and an agitator 54. The second culture vessel body 51 has a structure in which the upper portion is open.

The second culture vessel body 51 has a structure in which the upper portion is open. The heat insulating material 52 is installed on the outer side of the second culture vessel body 51 to serve to heat the second culture vessel body 51.

The cover member 53 includes a support frame 53a installed on the upper portion of the second culture vessel body 51 and a light shielding film 53b installed to cover the support frame 53a. The support frame 53a may include a reinforcing bar or a mesh net, and the light shielding film 53b is installed on the support frame 53a to cover the upper portion of the second culture vessel body 51. The light shielding film 53b may include a light shielding film or a giant to block 50% of sunlight from penetrating into the second culture vessel body 51.

While the stirrer 54 is rotated by wind power, the culture medium inside the second culture vessel body 51 is stirred, and has the same configuration as that of the stirrer 33 described above with reference to FIG. 3. Is omitted.

In addition, as shown in FIG. 1, the second culture tank 50 having the above configuration is connected to each of the first culture tanks 30 through a third connection pipe 114, and thus the first culture tank 30 is provided. Are fed phytoplankton cultured from The second culture tank 50 is installed to be paired with respect to each of the first culture tank (30).

The constant temperature culture room 90 is provided to supply the second culture tank (50) or to supply the second culture tank (30) by separately culturing only a high-purity animal plankton, and is pumped into the raw water tank (10) If the amount of zooplankton in the source water is low, supplement the zooplankton, or when the red or green algae in the water are serious, cultivate large amounts of pure zooplankton that are effective as natural enemies in the incubation room-> first culture-> second culture. It will be used as a device. In this case, the highly efficient zooplankton isolated from the laboratory was cultured in a constant temperature culture tank of 3.6 tons, and then increased to 12 tons of qhsoadj in the first culture tank (30), and then sent to the second culture tank (50) to increase the size to 24 tons. By discharging in the red tide or the green algae water, the control effect can be obtained.

As shown in FIGS. 5 and 6, the discharge device 80 uses the small anchor 81 and the buoy 82 so that the discharge pipe 83 is located at 0.5 to 1 m below the surface of the freshwater lake. Small ponds open toward the lake are formed to effectively discharge during low water levels.

The discharge device 80 includes a buoy 82 installed to float on the water surface, and a warning lamp 84 installed on the buoy 82 to be used as a safety device for preventing a ship accident and the like with the buoy 81. Equipped. In addition, the discharge device 80 is formed to be connected to each of the second culture tank 50 is cultured in the second culture tank 50 through the discharge pipe 83 is connected to and supported by the buoy 82 and the anchor Animal plankton can be supplied as fresh water. The outlet of the discharge pipe 83 may be provided with a mesh network for preventing the entry of fish.

Therefore, the zooplankton concentrated in the second culture tank 50 is supplied into the freshwater lake or the reservoir through the discharge device 80, and the supplied zooplankton concentrates the phytoplankton of the reservoir or the freshwater lake to concentrate the phytoplankton. This can effectively reduce the population.

In addition, the first or second culture tank (30, 50) may be installed in the interior of the building, it may be installed outdoors.

In addition, as shown in FIG. 7 as a culture tank according to another embodiment, it may include a pond facility 130 formed by digging the ground 100 to a predetermined depth. The pond facility 130 is made by digging the ground 100 to a predetermined depth to create a puddle 101 and cover the watertight film 131 to cover a portion of the puddle 101 and the ground (100) Can have The watertight membrane 131 may be fixed using a sand bag 133 or the like. The watertight membrane 131 may include a thick vinyl, a tent, or the like, and may be made of a material having watertightness to prevent the culture solution from flowing out or groundwater.

In addition, a cover member 135 is installed at the upper portion of the puddle 101. The cover member 135 may include a support frame 135a and a shielding film 135b installed on the support frame 135a. The support frame 135a may include a support skeleton made of reinforcing steel, wood, and the like, and the screening film 135b may include a vinyl, a tent, or the like for blocking rain. Pond facility 130 as described above may be installed to replace at least one of the first culture tank 30 and the second culture tank 50, and also the first and second culture tanks 30, 50 described above It can also be installed in combination.

The pond facility 130 is provided with an inlet tube 137 to which the plant or zooplankton culture solution is supplied, and a culture solution discharge tube 138 through which the culture solution containing the cultured phytoplankton or zooplankton is discharged.

Referring to the effect of the red tide and green algae causative organism and the protozoa predator culture apparatus capable of predating them according to the embodiment of the present invention having the above configuration as follows.

First, the pump 2 is driven to supply the raw water in the fresh water lake into the raw water tank 10 so that the raw water more than a predetermined amount is accommodated in the raw water tank 10.

Subsequently, the raw water in the raw water tank 10 is incubated while maintaining the predetermined temperature to purify the zooplankton and the phytoplankton so that they can be adapted indoors, that is, under the conditions of the culture tank. Where the raw water of the raw water tank 10 does not contain a sufficient amount of zooplankton, the high-purity animal plankton cultured in the incubation chamber 90 is supplied. Here, the constant temperature condition in the raw water tank 10 may be controlled to maintain a temperature of about 20 ℃.

Next, the raw water incubated in the raw water tank 10 is separately supplied to the first and second culture tanks 30 and 50, respectively. At this time, the filtration unit 20 passes only phytoplankton to move to the first culture tank 30, and animal plankton to move to the second culture tank (50).

The phytoplankton separated and moved to the first culture tank 30 is cultured through natural light while being maintained at a predetermined temperature. Phytoplankton introduced into the first culture tank 30 is cultured for about 3 days and then supplied to the second culture tank 50.

The zooplankton supplied to the second culture tank 50 is cultured for about 5 days, while intensively cultivating the phytoplankton cultured and supplied in the first culture tank 30. The second culture tank (50) by blocking the sunlight so that the zooplankton is actively cultured.

The zooplankton concentrated and cultured in the second culture tank 50 as described above is supplied through the discharge device 80 and supplied into the freshwater lake, so that the overgrown phytoplankton in the freshwater lake is fed to a predetermined level. It can be adjusted to hold.

In particular, when the red tide and green algae causative organisms having such a configuration and the protozoa predator cultivation apparatus capable of preying them are installed around Saemangeum lake, which is a representative freshwater lake in Korea, or around an agricultural reservoir inland, it frequently occurs in freshwater lakes. Red tide, green algae phenomenon can be effectively prevented.

Hereinafter, the contents of the pilot test (pilot test) to determine the effect of the red tide and algae causative organisms having the above configuration and the application of the culture apparatus of the protozoa predator that can feed them.

1. Culture Pilot Test Overview

 1) Planar layout of pilot: see FIG. 8

 2) Composition:-Container Material: Plastic Countertop

           Total capacity: 6 tons

           -Raw water input: A, B-> 1,38 ℓ / min

                           A-1, A-2, B-1, B-2-> 0.83 ℓ / min

           -Examination Schedule: May / 8 2008-June 10, 2008

           -Source of inflow: Munpo Port

2. Materials and Methods

 1) Experimental process: physicochemical analysis, quantitative analysis of phytoplankton and zooplankton by collecting water six times a week

       2) Analytical method: Phytoplankton was collected with a water collector and placed in a 500 ml polyethylene sample bottle and settled for about 2 weeks after fixation with Lugol solution (APHAAWWAWPCF, 1989). , 1978. Cell counts were determined using a Sedwick-Rafter counting chamber and examined at 400-1000 fold using an optical microscope, and species identification was followed by Mizuno (1964) and Hirose and Yamagishi (1977).

In the method of sampling the zooplankton, 10 L of water was collected and concentrated using a plankton net (mesh size 63 μm). The collected water was placed in a 250 ml polyethylene sample bottle, fixed in formalin at the site, transferred to a laboratory, and then counted by species using a 10-100 magnification dissecting microscope, and then the number of individuals per liter was converted. . For identification, species were observed on a Cobb slide containing lactophenol with 100-1000 times DIC microscope and the dissected species were dissected with anatomical pins and classified. The classification and identification of plankton were carried out with reference to the illustration of the Korean flora and fauna, the Japanese seawater / freshwater zooplankton search map, 水 野 (1964), Hirose and Yamogishi (1977), and 水 野 高橋 (1991).

3. Results

-Plan of cultures operated for this field application test

Pilot test to verify conditions as a kton culture and to examine its capacity

Was carried out.

1) Through the test, raw water was supplied to A and B through 80㎛ network, and it was found that phytoplankton, which is a food source of natural plankton, ie zooplankton, was cultured in the culture area.

2) Most of the zooplankton in A and B networks are supplied as A-1, A-2, B-1, and B-2, and it is designed as a device for mass culture of zooplankton in this culture.

3) In this pilot test, we examined the effect of plankton culture as originally designed device. The results are as follows.

      -. As a result, as can be seen with reference to Figs. 9a to 11b, in A and B, phytoplankton (phyto) was found to be 112-3364 cells / m 1.6-8.6 times more than 47-1740 cells / ml observed in raw water On the other hand, zooplankton (zoo) was caught by a filtration device, indicating that the population of 0-10 inds./l was found to be slightly less than 2-13 inds./l observed in raw water.

      In addition, phytoplankton was found to be 1-886 cells / ml in A-1, A-2, B-1, and B-2, which was significantly reduced compared to the number of cells observed in A and B. Is 15-299 inds. / ℓ 3-50 times more than the number of A and B cultures can be seen clearly that the function is effective in each culture.

In other words, this paper can be finally confirmed that the culture site installed for the field application test showed a distinct effect as a zooplankton culture facility.

1 is a block diagram showing a red tide causative organism and a protozoa predator culturing apparatus capable of predating them according to an embodiment of the present invention.

2 is a view showing the raw water tank of FIG.

3 is a view of FIG.

4 is a view showing a second culture tank of FIG.

5 and 6 each show the discharge device of FIG.

FIG. 7 shows a pond plant as another embodiment of the first and second culture baths. FIG.

Figure 8 shows the test culture structure for the pilot test.

9A and 9B are graphs showing the temperature and dissolved oxygen of each culture tank for testing for each point.

10A and 10B are graphs showing comparisons of phytoplankton and phytoplankton remaining amounts at respective points as of May 16, 2008.

11A and 11B are graphs showing comparisons of phytoplankton and phytoplankton remaining amounts at respective points as of May 29, 2008.

<Description of Symbols for Main Parts of Drawings>

10. Raw water tank 20. Filtration

30,50 .. First and second culture tanks 80 .. Discharge device

90. Constant temperature room 130. Pond facilities

Claims (11)

A raw water tank for storing raw water supplied from the freshwater lake; A filtering unit for separating phytoplankton and animal plankton contained in the raw water discharged from the raw water tank; At least one first culture tank for receiving and culturing phytoplankton filtered by the filtration unit; At least one second culture tank in which the phytoplankton cultured in the filtration unit is accommodated and cultured, and the phytoplankton cultured in the first culture tank is supplied; And It is installed to be immersed in the freshwater lake, and comprises a discharge device for discharging the culture solution containing the zooplankton cultured in the second culture tank to a pond or freshwater lake of a predetermined depth in the lake, Animal plankton cultured in the second culture tank is red tide and algae causative organisms, characterized in that discharged to the fresh water and protozoa predator culture apparatus capable of predating them. The method of claim 1, wherein the first culture tank, It can be cultured by receiving phytoplankton therein, and the first culture vessel body of the top is open and transparent or translucent material; Insulating material installed on the outside of the first culture vessel body; And Red stirrer and green algae causative organisms and protozoa predator culture apparatus capable of predating them, comprising: a stirrer for stirring the culture solution in the first culture tank body. The method of claim 2, wherein the stirrer, A stirrer body rotatably installed in the first culture tank; Red tide and green algae causative organisms and protozoa that can prey these, characterized in that it; is installed outside the first culture tank to provide a rotational force to rotate the stirrer body, and rotated by wind power Predator culture apparatus. According to claim 2, wherein the inner surface of the first culture tank body is provided with a light reflector for reflecting light, red tide and green algae organisms and protozoa predator culture apparatus capable of predating them. The method of any one of claims 1 to 4, wherein the second culture tank, A second culture vessel body capable of cultivating the zooplankton therein and having an open top; A cover member installed to cover the upper portion of the second culture tank body; Insulating material installed on the outside of the second culture vessel body; And Red stirrer and green algae causative organism and protozoa predator culture apparatus capable of predating them, comprising: a stirrer for agitating the culture solution inside the second culture tank using wind power. The method of claim 5, wherein the cover member comprises: a light shielding film for shielding sunlight; Red tide and green algae causative organisms and protozoa predator that can prey these, characterized in that it comprises; a support frame installed on the upper portion of the second culture tank body to support the light shielding film. The raw water tank according to any one of claims 1 to 4, Raw water tank body; A heater installed inside the raw water tank body; And a temperature control unit for sensing the raw water temperature inside the raw water tank main body and selectively controlling the driving of the heater based on the sensed temperature to maintain the raw water in the raw water tank main body at a constant temperature. Red tide and algae causative organisms and protozoa predator culture apparatus capable of predating them. The method of claim 1, wherein the first and second culture tanks, Red tide causal organisms and protozoa predator culture apparatus capable of preying them, characterized in that the pond adjacent to the freshwater lake artificially formed to a predetermined depth, so that the fresh water. The method according to any one of claims 1 to 4, Red tide and green algae causative organisms and predators are characterized in that it further comprises a constant-temperature culture room for culturing only the high-purity zooplankton and supplying it to the second culture tank or through the first and second culture tanks. Probiotic predator culture apparatus that can be. delete The method of claim 1, wherein the discharge device, A discharge pipe installed at a height of 0.5 to 1 m under water and floating so as to discharge the culture solution supplied to the second culture tank into a fresh water lake or a reservoir; An anchor connected to the discharge pipe and fixed to the bottom; A buoy installed to be exposed on the water surface connected to the discharge pipe; And Red and green algae causative organisms and protozoan predator culture apparatus capable of predating them, characterized in that it is installed in the buoy warning lights for facility safety.

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