CN101401578B - Uses of protopine in agricultural pesticide - Google Patents
Uses of protopine in agricultural pesticide Download PDFInfo
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- CN101401578B CN101401578B CN2008101435950A CN200810143595A CN101401578B CN 101401578 B CN101401578 B CN 101401578B CN 2008101435950 A CN2008101435950 A CN 2008101435950A CN 200810143595 A CN200810143595 A CN 200810143595A CN 101401578 B CN101401578 B CN 101401578B
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Abstract
The invention provides an application of protopine in pesticide bactericide, which uses the protopine as a main effective constituent in the aspect of pesticide bactericide. An inventor uses the protopine separately or compounds the protopine with other active constituents, and processes the protopine into wettable powder, emulsifiable solution, suspending agents, water dispersible granules, emulsions in water and other dosage forms having good effects in the aspect of bactericide by a preparation method.
Description
Technical field
The present invention relates to a kind of alkaloid with anti-microbial property prevents and treats crops and has good application aspect the disease.This a kind of alkaloid is the Biflorine (protopine) that belongs to protopine.
Technical background:
According to FAO (Food and Agriculture Organization of the United Nation) (FAO) statistics, the world food output due to illness loss that causes of insect pest accounts for 12% of gross output greatly every year, and a year amount of loss reaches 1,200 hundred million dollars, is equivalent to 1/2 of the Chinese agriculture gross output value, 1/3 of the U.S., 2 times of Japan, more than 4 times of Britain.The due to illness crop loss of worm harm 15% in China's every year, wherein grain is 3,500,000 tons, 900,000 tons in cotton, 2,800 ten thousand tons of vegetables, 3,000,000 tons of fruit, total value is roughly equal to more than 300 hundred million yuan.In the U.S. because the loss in damage by disease and insect every year on average is about 15% of agricultural production sum, above about 15,000,000,000 dollars.Just be to use the natural products agricultural chemicals for the appropriate methods of the control of diseases and pests of agronomic crop.
Before 2000 Christian eras, Homer (Alcaic) has just been write about " prevent insect pest, use sulphur, purify stifling under its gods' blessing ".China also is one of country that uses the earliest insecticide, bactericidal agent for preventing and treating plant pest, just uses mercurial arsenical and black false hellebore pest control before 1800.After 20th century invention forties organic chemistry agricultural chemicals, greatly strengthened the ability of the sick worm harm of human control, made great contribution for retrieving the crop yield loss.But, rely on for a long time and a large amount of Synthetic Organic Chemistry agricultural chemicals that use, brought a series of problems such as well-known environmental pollution, ecological balance destruction and food security.This more and more is subjected to the attention of scientists, and scientists turns to emphasis on those highly active natural agricultural chemicals.It seems its following tool special advantages now: one, because their contained active ingredient is natural materials, using the back has its degradation pathway smoothly at natural world, and environmental pollution is little; Two, to the people, animal low toxin.The application that is used for control of agricultural pest from active skull cap components is following pesticide developing main flow direction.
After first commercial seal chinaberry preparation Margosan-0 of U.S. Register in 1985, many seal chinaberry products have appearred in the world, as Azatin, Neemazid, Bio-neem, Align, Turplex, Bollwhip etc.; The product that with nicotine is main component in addition has Nico Soap, XL-ALL Noxfire Nicotin etc.; With the trifoliate jewelvine is Chem Sect, Cube Root, Noxfire, Potentox, the Prentox etc. of principal component.Domestic now successfully to have developed with nicotine, matrine, chinaberry element, fennel artemisin and Tea Saponin etc. be 26 plant species agricultural chemicals of principal component, and registered registration, and manufacturer reaches 102 families.
Application study to Biflorine is not a lot of at present, and the pertinent literature report has: human Biflorines (pmtopine) such as Jiang Liping and Han Xiaojian find that to the protective effect and the Mechanism Study of focal cerebral ischemia in rats, chmice acute cerebral ischemia and anoxia enduring it is to having anti-hypoxia, treating cerebral ischemia.People such as Fan Jishan find that Biflorine can suppress tracheal smooth muscle PDE activity, by cAMP level in the raising cell, and then lax tracheal smooth muscle effect.People such as Malaysian's justice find that Biflorine has the effect of the rabbit of inhibition platelet aggregation.Aspect patent: people such as Chen Biyi apply for a patent the application (01108424.3) of Biflorine in preparation treatment senile dementia disease drug.But also there is not Biflorine at the related application research report of pesticide sterilizing aspect antibacterial.
Biflorine mainly is present in plants such as Papaveraceae, Rutaceae and Berberidaceae, representative plant such as greater celandine, snowpoppy, macleaya cordata, macleaya microcarpa Fedde, decumbent corydalis tuber, heavenly bamboo etc.Do not see as yet yet and extract the application of Biflorine on bactericide agricultural chemicals in these plants.
208 ℃ of Biflorine fusing points.Be dissolved in chloroform, ethanol, ether, be slightly soluble in ethyl acetate, carbon disulphide, benzene, benzinum, water-soluble hardly.Molecular formula: C20H19N05 molecular weight: 353.37, structural formula:
Summary of the invention
The inventor carries out bioactivity research by agricultural chemicals pastille medium therapy (NY/T1156.2-2006) test method to it to Biflorine, finds that Biflorine has better bactericidal action, and concrete test is seen below.
Biflorine and moistened surface agent, bleeding agent and corresponding buffer and filler etc. are processed into pesticidal preparations and will have good market prospects aspect agriculture chemicals fungicides.
The objective of the invention is to propose a kind of non-selective agricultural chemicals, Biflorine is applied in the agriculture chemicals fungicide aspect.
Therefore, the present invention is by Biflorine being used to be prepared into the agricultural chemicals with bactericidal action, and produces good bactericidal action.
The Biflorine that the present invention mentioned is to be applied to sterilization pesticide, and acquisition or synthetic obtain from natural plants.
The application of Biflorine is to comprise independent use, perhaps uses jointly with other pesticide sterilizing active ingredients.When being used, also has good bactericidal action with other agricultural chemicals.
The invention provides a kind of agricultural chemicals, contain main bactericidal active ingredient Biflorine.
Described agricultural chemicals also can comprise the auxiliary material that auxiliary agent, filler etc. are conventional.
The bactericide agricultural chemicals that contains Biflorine of the present invention can be to comprising Chinese cabbage root rot, grey mould fruit rot of strawberry, and Chinese cabbage downy mildew, tobacco brown spot pathogen and Pyricularia oryzae have good killing action.
Agricultural chemicals of the present invention is applied to the exploitation of aspects such as bactericide for Biflorine is added the various formulations that pharmacy corresponding auxiliary material such as surfactant, buffer reagent and auxiliary material are made.Biflorine list usefulness or composite with other fungicide active ingredients can be processed into wetting powder, missible oil, suspending agent by formulation method, the application of formulations such as water dispersible granules and aqueous emulsion aspect bactericide has good effect.
Described pesticidal preparations is main bactericidal active ingredient with Biflorine, and the surfactant that is added can quicken active ingredient on the surface of leaf and stem or penetrate its diffusion into the surface.Cationics such as betain, amido betaine and pyrrolones; Anion surfactant is as sodium lauryl sulfate; Non-ionic surface active agent is as alkylolamides (FFA), fatty alcohol-polyoxyethylene ether (AE), alkylphenol polyoxyethylene (APE or OP), fatty acid, free fatty acid,, Tween-80, carboxymethyl cellulose, ethylene glycol, alkyl polyoxyethylene ether, alkyl polyoxyethylene ether, ethoxylation soya-bean oil, the castor-oil plant hydrogenated oil and fat, vegetable oil, silicone oil, methyl seed oil; And penetrating agent, as pyrroles, N-alkyl pyrrolones, pyrrolones and polyvinylpyrrolidone etc. methylate.These surfactants can use or mix use separately.
The Biflorine that the present invention mentions can extract to separate from natural plants and obtain, and also can obtain by synthetic.
The present invention is that main active is used for agriculture chemicals fungicide with the Biflorine, and at control Chinese cabbage root rot, grey mould fruit rot of strawberry, the disease due to Chinese cabbage downy mildew, tobacco brown spot pathogen and the Pyricularia oryzae etc. has good result.Have effectively, advantages such as low-residual, environmental protection.
In order better to understand essence of the present invention, below with test explanation more specifically.
One, Biflorine
Content 98% is provided by Hunan Engineering Research Center of Botanical Extract.
Two, bactericidal assay
1, bacterial classification: Chinese cabbage root rot, grey mould fruit rot of strawberry, Chinese cabbage downy mildew, tobacco brown spot pathogen and Pyricularia oryzae crop pathogens are provided by bio-safety science and technology institute of Agricultural University Of Hunan.
2, test method
Pastille medium therapy (NY/T1156.2-2006)
Above-mentioned Biflorine is dissolved with adequate amount of ethanol, contain the aqueous solution dilution of 0.1% Tween 80 then, being made into the Biflorine alkali content is 250mg/L, 125mg/mL, 62.5mg/L, 31.25mg/L, 15.6mg/L, 7.80mg/L, 3.90mg/L, gets the 1ml soup and adds the pastille culture medium flat plate of making different series concentration in the PDA quantitative culture medium that is cooled to 45 ℃.Make 6.5mm diameter mycelia piece from each sick all colony edge of medium 3-4d, move on each pastille medium, place 25 ℃ the biochemical incubator of constant temperature to cultivate, measure colony diameter behind the 4d, calculate growth inhibition ratio.Computing formula is as follows:
Growth inhibition ratio=[contrast colony diameter (mm)-processing colony diameter (mm)]/[contrast colony diameter (mm)-6.5] * 100
3, experimental result
| Bacterial classification | Biflorine EC 50(mg/L) |
| The Chinese cabbage pine root fungus | 28.80 |
| The grey mould fruit rot of strawberry bacterium | 22.60 |
| The Chinese cabbage downy mildew bacterium | 13.08 |
| Tobacco brown spot pathogen | 15.50 |
| Pyricularia oryzae | 10.20 |
The result shows: Biflorine all has different inhibition of degree or control efficiency to above bacterial classification.
Three, phytotoxicity test
1, to the toxicity test of strawberry
Above-mentioned Biflorine is made into 25,50,75,100,150,300 and 600ppm concentration solution be used to be sprayed at strawberry leaves, during dispenser for the first time, dispenser for the second time after 7 days, poisoning are observed and are lasted till that always dispenser for the second time is after 7 days.The result: it is withered that experimentation blade tip occurs without any a metering group, and big blade has poisoning phenomenons such as black splotch or vanelets are all withered.
2, to the toxicity test of tobacco
Above-mentioned Biflorine is made into 25,50,75,100,150,300 and 600ppm concentration solution be used to be sprayed at tobacco, during dispenser for the first time, dispenser for the second time after 7 days, poisoning are observed and are lasted till that always dispenser for the second time is after 7 days.The result: experimentation without any a metering group occur burning, symptom poisoning such as floral leaf, leaf roll, deformity, withered, death.
3, to the Chinese cabbage phytotoxicity test
Above-mentioned Biflorine is made into 25,50,75,100,150,300 and 600ppm concentration solution be used to be sprayed at Chinese cabbage, during dispenser for the first time, dispenser for the second time after 7 days, poisoning are observed and are lasted till that always dispenser for the second time is after 7 days.The result: experimentation blade band macula lutea, foxiness, withered spot etc. occur without any a metering group, symptoms such as withered, death.
The result shows that this series bactericidal agent contains Biflorine and in the 25-600ppm scope plant do not had toxic action.
Embodiment
Following examples are intended to illustrate the present invention rather than limitation of the invention further.
Embodiment one
Get (1) Biflorine 1.00% (2) castor-oil plant hydrogenated oil and fat 4.00% (3) Triton X-100 1.00% (4) octyl group pyrrolones 1.00% (5) ethanol 2.00% (6) water 91.00%.Make aqueous emulsion and be used to prevent and treat Alternaria alternate.Preparation dilution 1:50-100; The control rate of Alternaria alternate reaches more than 83%.
Embodiment two
Get (1) Biflorine 10.00% (2) polyethylene glycol 1.00% (3) sodium dihydrogen phosphate 2.00% (4) dodecyl sodium sulfate 10.00% (5) white carbon 5.00% (6) starch 7.00% (7) diatomite 65.00%.Make water dispersible granules, be used to prevent and treat the Chinese cabbage root rot.Preparation dilution 1:50-100; Chinese cabbage root rot control rate reaches more than 86%.
Embodiment three
Get (1) Biflorine 1.50% (2) carboxymethyl cellulose 0.50% (3) 1-methyl pyrrolidone 1.00% (4) nimbin 5.00% (4) acetone 5.00% (5) castor-oil plant hydrogenated oil and fat 7.00% (6) water 80.00%.Make aqueous emulsion and be used to prevent and treat the Chinese cabbage downy mildew bacterium, preparation dilution 1:50-100; The Chinese cabbage downy mildew control rate reaches more than 76%.
Embodiment four
Get (1) Biflorine 1.0% (2) Triton X-100 2.5% (3) Tea Saponin 10.0% (4) white sugar 2.0% (5) diatomite 84.5% and make wetting powder and be used to prevent and treat the grey mould fruit rot of strawberry bacterium, preparation dilution 1:100; The grey mould fruit rot of strawberry control rate reaches 83%.
Embodiment five
Get (1) Biflorine 1.00% (2) Tween-80 5.00% (3) water 15.00% (4) organosilicon 0.50% (5) rilanit special 78.5%.Make missible oil and be used to prevent and treat rice blast fungus, preparation dilution 1:100; The rice blast control rate reaches more than 76%.
Claims (4)
1. the application of Biflorine in agriculture chemicals fungicide is characterized in that, Biflorine is used to prepare the agricultural chemicals that disease due to Chinese cabbage root rot, grey mould fruit rot of strawberry, Chinese cabbage downy mildew or the tobacco brown spot pathogen is had preventive and therapeutic effect.
2. the application of Biflorine according to claim 1 in agriculture chemicals fungicide is characterized in that, Biflorine is to extract in the natural plants to separate to obtain, or synthetic obtains.
3. the application of Biflorine according to claim 1 in agriculture chemicals fungicide is characterized in that, its application mode comprises to be used Biflorine separately as bactericidal active ingredient, perhaps be used with other pesticide sterilizing active ingredients.
4. the application of Biflorine according to claim 1 in agriculture chemicals fungicide is characterized in that the described formulations of pesticide are wetting powder, missible oil, suspending agent, water dispersible granules, aqueous emulsion or aqua.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101435950A CN101401578B (en) | 2008-11-14 | 2008-11-14 | Uses of protopine in agricultural pesticide |
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| CN2008101435950A CN101401578B (en) | 2008-11-14 | 2008-11-14 | Uses of protopine in agricultural pesticide |
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| Publication Number | Publication Date |
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| CN101401578A CN101401578A (en) | 2009-04-08 |
| CN101401578B true CN101401578B (en) | 2011-04-20 |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107788015A (en) * | 2017-09-29 | 2018-03-13 | 杨凌农科大无公害农药研究服务中心 | A kind of wood tar and Macleaya cordata alkaloids composite bactericide, preparation method and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004095932A1 (en) * | 2003-04-28 | 2004-11-11 | Hermann Roth | Natural plant protection agent or repellent |
| CN1579387A (en) * | 2004-05-20 | 2005-02-16 | 北京神农坛医药科技有限公司 | Extraction of protopine from plant, and its manufacture of medicinal preparation and use |
| CN101073568A (en) * | 2006-05-15 | 2007-11-21 | 山东绿叶天然药物研究开发有限公司 | Use of medicine for treating or preventing heart failure |
| CN101352180A (en) * | 2008-09-12 | 2009-01-28 | 中国农业大学 | Macleaya cordata alkaloids for inhibiting plant pathogenic bacteria and preparation method thereof |
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- 2008-11-14 CN CN2008101435950A patent/CN101401578B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004095932A1 (en) * | 2003-04-28 | 2004-11-11 | Hermann Roth | Natural plant protection agent or repellent |
| CN1579387A (en) * | 2004-05-20 | 2005-02-16 | 北京神农坛医药科技有限公司 | Extraction of protopine from plant, and its manufacture of medicinal preparation and use |
| CN101073568A (en) * | 2006-05-15 | 2007-11-21 | 山东绿叶天然药物研究开发有限公司 | Use of medicine for treating or preventing heart failure |
| CN101352180A (en) * | 2008-09-12 | 2009-01-28 | 中国农业大学 | Macleaya cordata alkaloids for inhibiting plant pathogenic bacteria and preparation method thereof |
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