CN101392033B - Aloe polysaccharide extraction method and use thereof in cigarette - Google Patents
Aloe polysaccharide extraction method and use thereof in cigarette Download PDFInfo
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- CN101392033B CN101392033B CN2008102307108A CN200810230710A CN101392033B CN 101392033 B CN101392033 B CN 101392033B CN 2008102307108 A CN2008102307108 A CN 2008102307108A CN 200810230710 A CN200810230710 A CN 200810230710A CN 101392033 B CN101392033 B CN 101392033B
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Abstract
The invention discloses an extracting method of aloe polysaccharides, and the application thereof in cigarettes, wherein, the extracting method comprises the following steps: (1) fresh aloe leaves are crushed, centrifuged and filtered to obtain pure aloe raw juice; (2) after going through the steps of microwave extraction, centrifugal separation and concentration, water is added into alcohol, mixed, rested and filtered and crude aloe polysaccharides is obtained by settlement collection; (3) the filtrate goes through a nanofiltration membrane and an ultrafiltration membrane respectivcely for nanofiltration and ultrafiltration and then is frozen and dried; and (4) after being dissolved by water, the aloe polysaccharides is applied to tobacco shreds during a feeding process in a spraying manner so as to improve the moisturizing effect of the cigarettes; when being smoked, the dry sensation of the cigarettes is reduced, no undesirable odor exists in the smoke and the saliva of smokers is enriched. The extracting method has the obvious advantages that: the smoking quality of the cigarettes is improved; the content of harmful substances in the smoke is reduced; the sweetness and comfort are improved; the effects of enriching saliva and moisturizing are significant; and the application of the technology does not change the processing techniques of the existing cigarettes and has significance and value in the actual production.
Description
Technical field
The present invention relates to extraction and the application, particularly aloe polysaccharide extraction method of plant and in tobacco, use.This The Application of Technology does not change the existing complete processing of cigarette, can improve the sucking quality of cigarette.
Background technology
Along with China's rapid economy development, the cigarette human consumer is more and more higher to the requirement of product inner quality, and domestic all kinds of cigarette product is compared with human consumer's requirement, also have quality defect in various degree, as fragrance faint owe plentiful, odour characteristics not significantly, flue gas drying, pungent, thorn larynx, sweet degree is not enough, comfort level is relatively poor etc.Reduce the dry sensation of cigarette, the sucking quality that improves cigarette becomes the inexorable trend of cigarette development.
Aloe is subordinate to Liliaceae, for perennial evergreen meat herbaceous plant, is widely used in makeup, can play the effect of skin humectation.The main component that plays the humectation effect in definite at present aloe is an Aloe polysaccharide, and its kind has: (1 → 4) mannosans of glucomannan, mannosans, pectic acid and second phthaleinization.According to the Pharmacopoeia of the People's Republic of China (nineteen ninety version first one) record, Aloe polysaccharide has the effect of removing heat from the liver, defaecation.Modern study shows, effects such as aloe is antitumor in addition, antiviral, anti-ageing, enhancing body immunizing power.Domestic application about Aloe polysaccharide is not found the application precedent in tobacco mainly at aspects such as chemical constituents determination and makeup.
Summary of the invention
The present invention just is being based on above-mentioned present situation and is extracting at the Aloe polysaccharide in the aloe specially, and is applied in the tobacco, plays the pipe tobacco humectation, improves the purpose of the sucking quality of cigarette.
The objective of the invention is to be achieved through the following technical solutions: aloe polysaccharide extraction method of the present invention may further comprise the steps:
A, the wash clean Fresh leaf of aloe dried after, carry out the uv-radiation sterilization;
B, Fresh leaf of aloe is crushed into dilute slurry, carries out precipitation process with high speed precipitation machine with high speed disintegrator; Filter afterwards or vacuum filtration, promptly become the pure Normal juice of aloe;
C, the water that adds 1.5～3 times of amounts (v/v) in pure Normal juice extract, and industrial centrifugal machine is centrifugal with going up behind the extracting liquid filtering, gets supernatant liquor and is concentrated into 1/10～1/50 of original volume;
D, the supernatant liquor after will concentrating join in 90～100% ethanol, stir, leave standstill, filtered and recycled ethanol, collecting precipitation gets aloe coarse polysaccharid, filter cloth washes with water 3 times, washings merges with Crude polysaccharides, stirring and dissolving, the Crude polysaccharides aqueous solution, filter collection filtrate;
E, filtrate is undertaken promptly getting Aloe polysaccharide solution after nanofiltration, the ultrafiltration by 2000～3000 daltonian nanofiltration membrane and 5000～20000 daltonian ultra-filtration membranes respectively;
F, polysaccharide soln is carried out lyophilize handle, vacuum tightness is 600～750mmHg, and drying temperature is-22～35 ℃, and be 20～30h time of drying, promptly gets Aloe polysaccharide dry powder.
In the present invention, the used the most handy phreatic water of clear water of washing Fresh leaf of aloe can not be with the tap water that contains SYNTHETIC OPTICAL WHITNER, because of SYNTHETIC OPTICAL WHITNER can cause aloe liquid variable color, rotten.When extracting Aloe original juice, adopts water the method for microwave extracting.Using industrial centrifugal machine is with the 1000-2000 rev/min of centrifugal 15-20 of speed minute.
Aloe polysaccharide of the present invention when using, be with Aloe polysaccharide (solution or dry powder) after water dilution or dissolving, be applied on the pipe tobacco at the pipe tobacco charging process by spray pattern, cigarette humectation effect is obviously improved, and when burning and sucking cigarette, the cigarette dry sensation reduces, the flue gas free from extraneous odour has the effect of promoting the production of body fluid; The Aloe polysaccharide consumption is the 0.01-1.5% of pipe tobacco weight.
Outstanding advantage of the present invention is: the sucking quality that can improve cigarette, reduce content of harmful in the flue gas, sweet degree, comfort level improve, have significantly promote the production of body fluid, the humectation effect, and this The Application of Technology does not change the complete processing of existing cigarette, has the meaning and the value of actual production.
Pipe tobacco humectation measure of merit: with Aloe polysaccharide after water dissolution, be applied on the blank pipe tobacco at charging process by spray pattern, blank pipe tobacco applies the water of Isodose, it is positioned under the relative humidity 40% low wet environment in the lump, the result shows that the cigarette shreds humectation effect that applies Aloe polysaccharide obviously is better than blank pipe tobacco referring to pipe tobacco humectation measure of merit figure.
The smoking result of the blank cigarette that applies the cigarette of Aloe polysaccharide and do not apply sees Table 1,
Table 1, interpolation Aloe polysaccharide cigarette and blank cigarette contrast the experimental result of smokeing panel test
| Add kind | Fragrance | Assorted gas | Pungency | Strength | Pleasant impression |
| Aloe polysaccharide | Strong, substantial, the fragrance property sent out thoroughly is better | Little have | Have slightly | Moderate | More comfortable, the sense of promoting the production of body fluid is arranged |
| Contrast (blank) | Stronger, substantial, fragrance is sent out inadequately thoroughly | Have slightly | Heavy slightly | Moderate | Have residual slightly |
Description of drawings
Fig. 1 is the process flow sheet of extracting method of the present invention.
Fig. 2 is for adding the pipe tobacco humectation measure of merit figure of Aloe polysaccharide front and back.
Embodiment
The present invention is described further below in conjunction with embodiment, but is not restriction the present invention.
Embodiment 1
1, get Fresh leaf of aloe, clean with the phreatic water rinsing, dry, behind the 6min ultraviolet-sterilization, Fresh leaf of aloe is crushed into dilute slurry with high speed disintegrator.Carry out precipitation process with high speed precipitation machine then.Filter afterwards or vacuum filtration, promptly become the pure Normal juice of aloe.
2, with volume ratio be the water of 1:2, microwave extracting 10min with going up industrial centrifugal machine behind the extracting liquid filtering with 1000 rev/mins of centrifugal 15min of speed, gets supernatant liquor and is concentrated into 1/10 of original volume;
3, the supernatant liquor after will concentrating joins in 95% ethanol, stirs 5 minutes, leaves standstill filtered and recycled ethanol 2 hours, collecting precipitation gets aloe coarse polysaccharid, and filter cloth washes with water 3 times, and washings and Crude polysaccharides merge, stirring and dissolving, get the Crude polysaccharides aqueous solution, filter, collect filtrate;
4, filtrate is undertaken promptly getting polysaccharide soln after nanofiltration, the ultrafiltration by 2000 daltonian nanofiltration membrane and 10000 daltonian ultra-filtration membranes respectively.
5, polysaccharide soln is carried out lyophilize and handle, vacuum tightness is 700mmHg, and drying temperature is-30 ℃, and be 30h time of drying, gets polysaccharide dry powder.
6, with Aloe polysaccharide dry powder of the present invention after water dissolution, be applied on the cigarette shreds at charging process by spray pattern.Concrete usage ratio is: the Aloe polysaccharide consumption is 0.5% of a pipe tobacco weight.
Embodiment 2
1, get Fresh leaf of aloe, clean with the phreatic water rinsing, dry, behind the 8min ultraviolet-sterilization, Fresh leaf of aloe is crushed into dilute slurry with high speed disintegrator.Carry out precipitation process with high speed precipitation machine then.Filter afterwards or vacuum filtration, promptly become the pure Normal juice of aloe.
2, with volume ratio be the water of 1:3, microwave extracting 20min with going up industrial centrifugal machine behind the extracting liquid filtering with 2000 rev/mins of centrifugal 15min of speed, gets supernatant liquor and is concentrated into 1/20 of original volume;
3, the supernatant liquor after will concentrating joins in 98% ethanol, stirs 8 minutes, leaves standstill filtered and recycled ethanol 3 hours, collecting precipitation gets aloe coarse polysaccharid, and filter cloth washes with water 3 times, and washings and Crude polysaccharides merge, stirring and dissolving, get the Crude polysaccharides aqueous solution, filter, collect filtrate;
4, filtrate is undertaken promptly getting polysaccharide soln after nanofiltration, the ultrafiltration by 3000 daltonian nanofiltration membrane and 20000 daltonian ultra-filtration membranes respectively.
5, polysaccharide soln is carried out lyophilize and handle, vacuum tightness is 750mmHg, and drying temperature is-35 ℃, and be 25h time of drying, gets polysaccharide dry powder.
6, Aloe polysaccharide dry powder of the present invention (also can directly be diluted the back with the Aloe polysaccharide solution with water) after water dissolution, be applied on the cigarette shreds at charging process by spray pattern.Concrete usage ratio is: the Aloe polysaccharide consumption is 1% of a pipe tobacco weight.
Claims (3)
1. the application of Aloe polysaccharide in cigarette, it is characterized in that: with Aloe polysaccharide after water dissolution, be applied on the pipe tobacco at the pipe tobacco charging process by spray pattern, the Aloe polysaccharide consumption is the 0.01-1.5% of pipe tobacco weight, and described Aloe polysaccharide prepares by following steps:
A, the wash clean Fresh leaf of aloe dried after, carry out the uv-radiation sterilization;
B, Fresh leaf of aloe is crushed into dilute slurry, carries out precipitation process with high speed precipitation machine with high speed disintegrator; Filter afterwards, promptly become the pure Normal juice of aloe;
C, the water that adds 1.5～3 times of amounts (v/v) in pure Normal juice extract, and industrial centrifugal machine is centrifugal with going up behind the extracting liquid filtering, gets supernatant liquor and is concentrated into 1/10～1/50 of original volume;
D, the supernatant liquor after will concentrating join in 90～100% ethanol, stir, leave standstill, filtered and recycled ethanol, collecting precipitation gets aloe coarse polysaccharid, filter cloth washes with water, washings merges with Crude polysaccharides, stirring and dissolving, the Crude polysaccharides aqueous solution, filter collection filtrate;
E, filtrate is undertaken promptly getting Aloe polysaccharide solution after nanofiltration, the ultrafiltration by 2000～3000 daltonian nanofiltration membrane and 5000～20000 daltonian ultra-filtration membranes respectively;
F, polysaccharide soln is carried out lyophilize handle, vacuum tightness is 600～750mmHg, and drying temperature is-22～-35 ℃, and be 20～30h time of drying, promptly gets Aloe polysaccharide dry powder.
2. the application of Aloe polysaccharide according to claim 1 in cigarette is characterized in that: the method that adopts microwave extracting when water extracts Aloe original juice.
3. the application of Aloe polysaccharide according to claim 1 in cigarette is characterized in that: industrial centrifugal machine was with the 1000-2000 rev/min of centrifugal 15-20 of speed minute.
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| CN2008102307108A CN101392033B (en) | 2008-11-03 | 2008-11-03 | Aloe polysaccharide extraction method and use thereof in cigarette |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101845101B (en) * | 2010-06-22 | 2012-04-25 | 中国烟草总公司郑州烟草研究院 | Preparation of undaria pinnatifida rough polysaccharide and application thereof in cigarette |
| CN103126063B (en) * | 2011-11-23 | 2016-02-17 | 福建中烟工业有限责任公司 | One grows tobacco sense organ humectant and the using method in tobacco thereof |
| GB201213870D0 (en) | 2012-08-03 | 2012-09-19 | British American Tobacco Co | Tobacco extract, preparation thereof |
| CN102793263B (en) * | 2012-08-27 | 2016-01-20 | 福建中烟工业有限责任公司 | The extracting method of white fungus water extracting liquid and the application in tobacco thereof |
| CN102796204A (en) * | 2012-08-27 | 2012-11-28 | 福建中烟工业有限责任公司 | Extraction method of tremella polysaccharide and application of tremella polysaccharide in tobaccos |
| CN103045370B (en) * | 2012-12-28 | 2014-12-24 | 华宝食用香精香料（上海）有限公司 | Method for preparing cigarette essence containing aloe extractive and application of cigarette essence to cigarettes |
| CN103504463B (en) * | 2013-09-27 | 2015-09-30 | 广东中烟工业有限责任公司 | The application of a kind of shaddock ped polysaccharide in tobacco leaf humectation |
| CN106590931A (en) * | 2016-12-14 | 2017-04-26 | 江西中烟工业有限责任公司 | Preparation method of cigarette protein-polysaccharide composite emulsion carrier system and application of carrier system in cigarettes |
| CN107034018A (en) * | 2017-04-27 | 2017-08-11 | 河南中烟工业有限责任公司 | A kind of quick-fried pearl aloe flavour of cigarette and its application in cigarette |
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| WO2003057893A1 (en) * | 2002-01-02 | 2003-07-17 | Unigen Pharmaceuticals, Inc. | Process for the preparation of immunomodulatory polysaccharides from aloe |
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| US20050019433A1 (en) * | 2001-12-27 | 2005-01-27 | Willem Van Dijk | Negatively charged polysaccharide derivable from aloe vera |
| CN1208348C (en) * | 2002-06-26 | 2005-06-29 | 湖南金正方生物科技有限公司 | Process for extracting aloinose |
| CN1948346A (en) * | 2005-10-10 | 2007-04-18 | 云南元江万绿生物（集团）有限公司 | Fractional extraction method of aloe polysaccharide |
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| US20050019433A1 (en) * | 2001-12-27 | 2005-01-27 | Willem Van Dijk | Negatively charged polysaccharide derivable from aloe vera |
| WO2003057893A1 (en) * | 2002-01-02 | 2003-07-17 | Unigen Pharmaceuticals, Inc. | Process for the preparation of immunomodulatory polysaccharides from aloe |
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