CN1424330A - Extraction of aloe polysaccharide - Google Patents
Extraction of aloe polysaccharide Download PDFInfo
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- CN1424330A CN1424330A CN 02159727 CN02159727A CN1424330A CN 1424330 A CN1424330 A CN 1424330A CN 02159727 CN02159727 CN 02159727 CN 02159727 A CN02159727 A CN 02159727A CN 1424330 A CN1424330 A CN 1424330A
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Abstract
A process for extracting aloe polyose from the raw aloe juice features use of membrane separation, nano filter and ultrafilter. Its advantages are not degradable easily and high bioactivity and output rate.
Description
Technical field
The present invention relates to the preparation method of Aloe polysaccharide.
Background technology
Aloe is the perennial fleshiness herbaceous plant of Liliaceae, originate in Africa, because of its magical effect, extensively propagated by people all over the world, now rise to each kind biological product and pharmaceutical raw material from simple raw material as food, healthcare products, skin care product and beverage.
Activeconstituents in the aloe is distributed in two different positions: gel and yellow juice.Mainly has a thermo-sensitivity polysaccharide in the gel by what mannosans or glucomannan were formed.It is the important component that aloe has pharmaceutical activity, that its main effect to human body has is antiviral, enhance immunity power, antitumor, promote wound healing, treatment radiation burn and to effects such as the preserving moisture of skin, nutrition, reparations.
At present, also there is not the sophisticated method that from Aloe original juice, prepares Aloe polysaccharide.
Summary of the invention
The purpose of this invention is to provide a kind of method of from Aloe original juice, extracting Aloe polysaccharide, obtain the Aloe polysaccharide in different molecular weight interval.
For achieving the above object, the present invention takes following scheme:
At first Aloe original juice is dropped into and carry out the nanofiltration processing in the nanofiltration device, the aperture of the used separatory membrane of nanofiltration process is 1000～2000 dalton, small-molecule substance is seen through, and the material of intermediate molecular weight and macromolecule can not see through; The filtrate that can not see through the nanofiltration device then adds ultra-fine filter and carries out two-step approach membrane sepn (being uf processing), and the aperture of separatory membrane is 3000～6000 dalton for the first time, and the material of intermediate molecular weight is passed through; The aperture of separatory membrane is 10000～20000 dalton for the second time, and the material less than this molecular weight is passed through; Then the polysaccharide liquid after the uf processing being carried out lyophilize respectively handles, during processing, vacuum tightness is 650～750mmHg, and drying temperature is-30 ℃～-20 ℃, 30～40 hours time of drying, can obtain the Aloe polysaccharide powder in large, medium and small three grades of different molecular weight intervals.
Advantage of the present invention is: provide a kind of and utilize that membrane separation technique is separated, the method for purification Aloe polysaccharide from Aloe original juice.Utilize the present invention can obtain the Aloe polysaccharide freezing dry powder in different molecular weight interval, the Aloe polysaccharide of different molecular weight can be used as raw material and adds to different in product.Wherein the polysaccharide of small molecular weight can be used as material additive and adds in food, the makeup, effect such as reach nutrition, preserve moisture, and the polysaccharide of macromolecule since have good enhancing body resistibility, antitumor, antiviral, promote to can be used as medical functions such as wound healing raw material and to add biological products, medicine, healthcare products to.In addition, present method is carried out under normal temperature and low temperature, has avoided the decomposition of Aloe polysaccharide effectively.
Description of drawings
Fig. 1 is the uf processing schema of the embodiment of the invention 1
Fig. 2 is the infrared spectrogram of the macromolecule powder of the embodiment of the invention 1 gained
Embodiment
At first Aloe original juice 50kg is dropped into and carry out the nanofiltration processing in the nanofiltration device, the aperture of the used separatory membrane of nanofiltration process is 1000 dalton, can make molecular weight less than 1000 daltonian material permeances, and collection can not see through the filtrate of nanofiltration device; The filtrate that can not see through the nanofiltration device then adds ultra-fine filter and carries out uf processing, as shown in Figure 1.The aperture of separatory membrane is 4000 dalton for the first time, and the aperture of separatory membrane is 10000 dalton for the second time, and the material less than this molecular weight is passed through; Then the polysaccharide liquid after the uf processing being carried out lyophilize respectively handles, during processing, vacuum tightness is 750mmHg, drying temperature is-30 ℃, 30 hours time of drying, can obtain big (molecular weight is greater than 10000 dalton), in each 500g of Aloe polysaccharide powder in (molecular weight is between 4000 to 10000), little (molecular weight is between 1000 to 4000) three grades of different molecular weight intervals.
The macromolecule powder of gained is carried out Infrared spectroscopy, and its spectrogram is seen Fig. 2.As shown in Figure 2, products therefrom really is an Aloe polysaccharide.
Compare with embodiment 1, embodiment 2～4 has changed the parameter when lyophilize is handled, the aperture of each separatory membrane when embodiment 5～8 has changed nanofiltration processing and uf processing, and it the results are shown in the table 1 of nextpage.
Table 1
| Numbering | (dalton) handled in nanofiltration | Uf processing (dalton) | Lyophilize is handled | Product (Aloe polysaccharide powder) (g) | |||||
| For the first time | For the second time | Vacuum tightness (mmHg) | Temperature (℃) | Time (hr) | Small molecular weight | Middle molecular weight | Macromolecule | ||
| Example 1 | ??1000 | ??4000 | ??10000 | ????750 | ????-30 | ????30 | ????500 | ????500 | ????500 |
| Example 2 | ??1000 | ??4000 | ??10000 | ????650 | ????-30 | ????30 | ????480 | ????460 | ????490 |
| Example 3 | ??1000 | ??4000 | ??10000 | ????750 | ????-30 | ????40 | ????490 | ????475 | ????492 |
| Example 4 | ??1000 | ??4000 | ??10000 | ????750 | ????-40 | ????30 | ????480 | ????469 | ????481 |
| Example 5 | ??2000 | ??4000 | ??10000 | ????750 | ????-30 | ????30 | ????410 | ????501 | ????495 |
| Example 6 | ??1000 | ??3000 | ??10000 | ????750 | ????-30 | ????30 | ????500 | ????426 | ????570 |
| Example 7 | ??1000 | ??6000 | ??10000 | ????750 | ????-30 | ????-30 | ????700 | ????310 | ????493 |
| Example 8 | ??1000 | ??4000 | ??20000 | ????750 | ????-30 | ????-30 | ????498 | ????498 | ????688 |
Claims (4)
1, a kind of method of extracting Aloe polysaccharide from Aloe original juice is characterized in that, comprises following steps:
A. Aloe original juice is dropped into and carry out the nanofiltration processing in the nanofiltration device, the aperture of the used separatory membrane of nanofiltration process is 1000～2000 dalton, small-molecule substance is seen through, and the material of intermediate molecular weight and macromolecule can not see through; Then
The filtrate that B. can not see through the nanofiltration device adds ultra-fine filter and carries out the two-step approach membrane sepn, and the aperture of separatory membrane is 3000～6000 dalton for the first time, and the material of intermediate molecular weight is passed through; The aperture of separatory membrane is 10000～20000 dalton for the second time, and the material less than this molecular weight is passed through; Then
C. the polysaccharide liquid that obtains among the step B is carried out lyophilize respectively and handle, during processing, vacuum tightness is 650～750mmHg, and drying temperature is-30 ℃～-20 ℃, 30～40 hours time of drying, can obtain the Aloe polysaccharide powder in different molecular weight interval.
2, the method for utilizing membrane separation technique to extract Aloe polysaccharide from Aloe original juice according to claim 1 is characterized in that: in the described nanofiltration treating processes, the aperture of separatory membrane is 1000 dalton.
3, the method for utilizing membrane separation technique from Aloe original juice, to extract Aloe polysaccharide according to claim 1, it is characterized in that: in the described process of ultrafiltration treatment, the aperture of separatory membrane is 4000 dalton for the first time, and the aperture of separatory membrane is 10000 dalton for the second time.
4, the method for utilizing membrane separation technique to extract Aloe polysaccharide from Aloe original juice according to claim 1 is characterized in that: in the described freezing dry process, vacuum tightness is 750mmHg, and drying temperature is-30 ℃, 40 hours time of drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02159727 CN1424330A (en) | 2002-12-30 | 2002-12-30 | Extraction of aloe polysaccharide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02159727 CN1424330A (en) | 2002-12-30 | 2002-12-30 | Extraction of aloe polysaccharide |
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| CN1424330A true CN1424330A (en) | 2003-06-18 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101070354B (en) * | 2007-05-18 | 2010-04-07 | 中南大学 | Method for synchronous producing aloe polysaccharide powder and aloe active water using aloes |
| CN102101893A (en) * | 2010-12-20 | 2011-06-22 | 大兴安岭林格贝有机食品有限责任公司 | Method for enriching and purifying aloe polysaccharides in aloe |
| CN101392033B (en) * | 2008-11-03 | 2011-12-21 | 中国烟草总公司郑州烟草研究院 | Aloe polysaccharide extraction method and use thereof in cigarette |
| CN103463378A (en) * | 2013-09-09 | 2013-12-25 | 开封九福来生物技术有限公司 | Method for preparing aloe injection by using aloe liquid |
| CN103554294A (en) * | 2013-11-19 | 2014-02-05 | 海南钟晨生物工程有限责任公司 | Method for processing high-O-acetyl-content raw aloe product |
| CN105588905A (en) * | 2015-08-13 | 2016-05-18 | 北京工商大学 | Fingerprint spectrum establishment method and standard fingerprint spectrum of aloe polysaccharides |
| CN106265888A (en) * | 2015-05-22 | 2017-01-04 | 天津市医药科学研究所 | Herba Sophorae alopecuroidis polysaccharide composition and its preparation method and application |
| CN106456525A (en) * | 2014-04-17 | 2017-02-22 | 荷兰联合利华有限公司 | Aloe vera extract for personal care compositions |
| US10456353B2 (en) | 2014-04-17 | 2019-10-29 | Conopco, Inc. | Personal care compositions |
-
2002
- 2002-12-30 CN CN 02159727 patent/CN1424330A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101070354B (en) * | 2007-05-18 | 2010-04-07 | 中南大学 | Method for synchronous producing aloe polysaccharide powder and aloe active water using aloes |
| CN101392033B (en) * | 2008-11-03 | 2011-12-21 | 中国烟草总公司郑州烟草研究院 | Aloe polysaccharide extraction method and use thereof in cigarette |
| CN102101893A (en) * | 2010-12-20 | 2011-06-22 | 大兴安岭林格贝有机食品有限责任公司 | Method for enriching and purifying aloe polysaccharides in aloe |
| CN102101893B (en) * | 2010-12-20 | 2012-08-22 | 大兴安岭林格贝有机食品有限责任公司 | Method for enriching and purifying aloe polysaccharides in aloe |
| CN103463378A (en) * | 2013-09-09 | 2013-12-25 | 开封九福来生物技术有限公司 | Method for preparing aloe injection by using aloe liquid |
| CN103554294B (en) * | 2013-11-19 | 2016-05-04 | 海南钟晨生物工程有限责任公司 | A kind of high-load O-acetyl group raw material aloe product processing method |
| CN103554294A (en) * | 2013-11-19 | 2014-02-05 | 海南钟晨生物工程有限责任公司 | Method for processing high-O-acetyl-content raw aloe product |
| CN106456525A (en) * | 2014-04-17 | 2017-02-22 | 荷兰联合利华有限公司 | Aloe vera extract for personal care compositions |
| US10449143B2 (en) | 2014-04-17 | 2019-10-22 | Conopco, Inc. | Aloe vera extract for personal care compositions |
| US10456353B2 (en) | 2014-04-17 | 2019-10-29 | Conopco, Inc. | Personal care compositions |
| CN106456525B (en) * | 2014-04-17 | 2019-12-03 | 荷兰联合利华有限公司 | Aloe extract for personal care composition |
| CN106265888A (en) * | 2015-05-22 | 2017-01-04 | 天津市医药科学研究所 | Herba Sophorae alopecuroidis polysaccharide composition and its preparation method and application |
| CN105588905A (en) * | 2015-08-13 | 2016-05-18 | 北京工商大学 | Fingerprint spectrum establishment method and standard fingerprint spectrum of aloe polysaccharides |
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