CN101361846A - Detoxification fever-reduction traditional Chinese medicine for treating internal medical excess fire disease and production method thereof - Google Patents
Detoxification fever-reduction traditional Chinese medicine for treating internal medical excess fire disease and production method thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine for detoxification, clearing heat and curing internal sthenia fire diseases and a preparation method thereof; the traditional Chinese medicine has obvious clinical effects in curing inflammations caused by a plurality of pathogenies, epidemic febrile disease at early stage and internal and external febrile symptoms; five medical herbs in the traditional Chinese medicine of the invention can perform a collaborative function, thus being capable of eliminating external and internal heats and heat in triple warmer. The traditional Chinese medicine of the invention is prepared by the following medical materials: 12 to 15 weight proportions of rhizoma coptidis, 18 to 24 weight proportions of radix scutellariae, 11 to 16 weight proportions of phellodendron, 24 to 29 weight proportions of honeysuckle and 19 to 27 weight proportions of forsythia suspensa vahl. The technical steps are: the forsythia suspensa vahl and the honeysuckle are added with water for double decoctions, wherein, the first decoction lasts for 1.5 hours and the second decoction lasts for 1 hour; the phellodendron is added with water for double decoctions, wherein, both decoctions last for two hours; the decoction of the forsythia suspensa vahl and the honeysuckle as well as the decoction of the phellodendron are combined, filtered, decompressed and concentrated to an extractum; the rhizoma coptidis and the radix scutellariae are crushed into fine powder; the powder and the extractum are mixed, dried, crushed, screened, palletized, compressed into tablets and coated to obtain the traditional Chinese medicine of the invention.
Description
One, technical field:
The present invention relates to a kind of Chinese medicine, especially relate to a kind of detoxifcation heat clearing Chinese medicine and manufacture method that is used for the treatment of internal medicine excess-fire card class.
Two, background technology:
The tender genus of children's's body constitution " pure sun " is easy to being invaded by exogenous pathogen, and poison is gone into heresy, and heat-transformation changes very fast, and evil poison invasion heat-transformation causes multiple warm disease, is usual diseases of childhood machine characteristics, and the heat clearing away of therefore detoxifying is the basic rule of treatment of the warm disease of treatment children's.Existing Chinese medicine and the Western medicine curative effect that is used for the treatment of the warm disease of children's is long, and therapeutic effect is not obvious.
Three, summary of the invention:
The object of the present invention is to provide a kind of detoxifcation heat clearing Chinese medicine and manufacture method that is used for the treatment of internal medicine excess-fire card class, it all has significant clinical efficacy to the inflammation due to the Different types of etiopathogenises, to epidemic febrile disease from the beginning of, and the exterior and the interior heat symptom-complex has significant curative effect, five kinds of medicines all can be brought into play synergism, messenger drug is the exterior and the interior no matter, and the heat of three warmers all can be dispeled.
For achieving the above object, the technical solution used in the present invention is:
A kind of detoxifcation heat clearing Chinese medicine that is used for the treatment of internal medicine excess-fire card class, its special character is to be made by the following weight parts crude drug: Rhizoma Coptidis 12-15 weight portions, Radix Scutellariae 18-24 weight portions, Cortex Phellodendri 11-16 weight portions, Flos Lonicerae 24-29 weight portions, Fructus Forsythiae 19-27 weight portions.
A kind of manufacture method that is used for the treatment of the detoxifcation heat clearing Chinese medicine of internal medicine excess-fire card class, it is characterized in that comprising following processing step: Fructus Forsythiae, Flos Lonicerae decoct with water secondary, are respectively 1.5 hours, 1 hour; Cortex Phellodendri decocts with water secondary, each 2 hours; Collecting decoction, filtration, filtrate decompression are concentrated into the thick paste that relative density is 1.30-1.35 (50 ℃); Rhizoma Coptidis, Radix Scutellariae powder are broken into fine powder, add mixing in the thick paste, and drying is pulverized, sieve, and granulation, tabletting, sugar coating is promptly.
Compared with prior art, the advantage and the effect that have of the present invention is as follows:
1, some of Chinese medicine compatibilities commonly used and tradition name side between their composition, often show as Synergistic, reduce drug resistance and form or reduce beneficial effects such as side reaction.Share as Flos Lonicerae and Fructus Forsythiae, is exactly an example, and to the research of a large amount of compound recipes that contains Rhizoma Coptidis, the Rhizoma Coptidis compound recipe has stronger heat-clearing and toxic substances removing than the single Rhizoma Coptidis and renders a service.Radix Scutellariae, Rhizoma Coptidis, Cortex Phellodendri all have the merit of clearing away heat-fire detoxifcation in the side, and Radix Scutellariae can expelling pathogenic factors from muscles with clear purte the white move back pathogenic heat, Rhizoma Coptidis eliminating heart-fire and the contrary dysentery of the kind stopping vomiting of relieving restlessness, Cortex Phellodendri is rushed down the kidney fire and the kind clear part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels of YIN nourishing damp and hot, therefore ancients have Radix Scutellariae to control the part of the body cavity above the diaphragm housing the heart and lungs, and Rhizoma Coptidis is controlled middle Jiao, and Cortex Phellodendri is controled the inferior saying of Jiao.Being monarch drug with the Rhizoma Coptidis among the we, is ministerial drug with Radix Scutellariae, Cortex Phellodendri, and the monarch and his subjects' compatibility is played heat-clearing and toxic substances removing altogether, removes the effect of three warmers pathogenic heat.Flos Lonicerae, sweet cold Chhnang and go into lung meridian not only kindly is dispersed in the heat of the pathogenic heat of table but also the stomach that can clear away heart-fire and detoxifies; Fructus Forsythiae, the light clear come-up of bitter cold, the pathogenic heat of the exterior and the interior that can loose thoroughly and the heat clearing away of inducing sweat, Flos Lonicerae, Fructus Forsythiae all have heat-clearing and toxic substances removing, the feature of the saturating the exterior and the interior that looses can diathermanously reach table, again interior-heat and detoxifying clearly, YINQIAO two flavors all have to rise to float declares diffusing property, with the product that are heat-clearing and toxic substances removing, the pyretic toxicity pathogenic factor is separated from table.This two flavor is both as the usefulness of ministerial drug and adjuvant drug, the real double again property of medicine that makes.Five medicines cooperate, and can better bring into play synergism, Flos Lonicerae, and Fructus Forsythiae can rush down stomach-fire in middle Jiao with Rhizoma Coptidis again with the Radix Scutellariae purte the white in the part of the body cavity above the diaphragm housing the heart and lungs, also can be with the Cortex Phellodendri reducing the minister-fire in the part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels.SANHUANG, again by Flos Lonicerae, Fructus Forsythiae rise power messenger drug that floating a surname looses nowhere less than, make that burning hot poison is evil to be separated from table, the exterior and the interior no matter, the heat of three warmers all can be removed it.
2, the present invention is through clinical verification, and total effective rate is 97.23%.Radix Scutellariae, Rhizoma Coptidis, the detoxifcation of Cortex Phellodendri clearing away heat-fire in the side, Radix Scutellariae can move back pathogenic heat by expelling pathogenic factors from muscles with clear purte the white, the contrary dysentery of the kind stopping vomiting of Rhizoma Coptidis removing pathogen in the heart and relieving restlessness, Cortex Phellodendri rush down the kidney fire and the kind clear part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels of YIN nourishing damp and hot, sweet cold the lightly floating into lung of Flos Lonicerae, if Fructus Forsythiae is cold light clear come-up, the pathogenic heat of the exterior and the interior of loosing thoroughly and the heat clearing away of inducing sweat, five kinds of medicines all can be brought into play synergism, and messenger drug is the exterior and the interior no matter, the heat of three warmers all can be dispeled.Product is a sugar-coat extractum sheet, ratify through Department of Public Health of Shanxi Province, in the hospital of traditional Chinese hospital, Xi'an, units such as city maternity and child care center, Xincheng District institute of traditional Chinese medicine carry out the observation of curative effect of 314 routine patients (wherein 80 examples are adults), proof Sanhuang Qingjie sheet to epidemic febrile disease from the beginning of, and the exterior and the interior heat symptom-complex has significant curative effect, total effective rate is 94.2%, and wherein recovery from illness accounts for 79.3% of sum.Taking medicine got final product onset in back one day, and only need 2-5 day promptly to heal the general course of treatment.Do not see untoward reaction and other side effect.The present invention is a sugar-coat extractum sheet, and the tablet of the whole crude drugs of consumption is little, and suitable child administration technology is reasonable, is convenient to promote.Quality standard has all carried out qualitative test and micro-difference to full constituent in the prescription, can control drug quality more effectively.Product is not only to children's, and is the active drug of a kind of heat-clearing and toxic substances removing, anti-inflammation per capita to becoming.
3, the present invention can realize standardization, large-scale production, constant product quality, reliable.
Four, the specific embodiment:
Embodiment one: the traditional method of learning from else's experience collection processing is concocted: Rhizoma Coptidis 12.6 weight portions, Radix Scutellariae 21.3 weight portions, Cortex Phellodendri 13.3 weight portions, Flos Lonicerae 26.7 weight portions, Fructus Forsythiae 26.1 weight portions.Above five tastes Fructus Forsythiae, Flos Lonicerae decoct with water secondary, are respectively 1.5 hours, 1 hour.Cortex Phellodendri decocts with water secondary, each 2 hours.Collecting decoction, filtration, filtrate decompression are condensed into extractum.Rhizoma Coptidis, Radix Scutellariae powder are broken into fine powder, add mixing in the thick paste, and drying is pulverized, sieve, and granulation, tabletting, coating is promptly.
Embodiment two: the traditional method of learning from else's experience collection processing is concocted: Rhizoma Coptidis 13.6 weight portions, Radix Scutellariae 23.5 weight portions, Cortex Phellodendri 14.8 weight portions, Flos Lonicerae 26.7 weight portions, Fructus Forsythiae 21.4 weight portions.Above five tastes Fructus Forsythiae, Flos Lonicerae decoct with water secondary, are respectively 1.5 hours, 1 hour.Cortex Phellodendri decocts with water secondary, each 2 hours.Collecting decoction, filtration, filtrate decompression are condensed into extractum.Rhizoma Coptidis, Radix Scutellariae powder are broken into fine powder, add mixing in the thick paste, and drying is pulverized, sieve, and granulation, tabletting, coating is promptly.
Embodiment three: the traditional method of learning from else's experience collection processing is concocted: Rhizoma Coptidis 13.3 weight portions, Radix Scutellariae 20.0 weight portions, Cortex Phellodendri 13.3 weight portions, Flos Lonicerae 26.7 weight portions, Fructus Forsythiae 26.7 weight portions.Above five tastes Fructus Forsythiae, Flos Lonicerae decoct with water secondary, are respectively 1.5 hours, 1 hour.Cortex Phellodendri decocts with water secondary, each 2 hours.Collecting decoction, filtration, filtrate decompression are condensed into extractum.Rhizoma Coptidis, Radix Scutellariae powder are broken into fine powder, add mixing in the thick paste, and drying is pulverized, sieve, and granulation, tabletting, coating is promptly.
Embodiment four: the traditional method of learning from else's experience collection processing is concocted: Rhizoma Coptidis 14.5 weight portions, Radix Scutellariae 23.5 weight portions, Cortex Phellodendri 13.8 weight portions, Flos Lonicerae 28.5 weight portions, Fructus Forsythiae 19.7 weight portions.Above five tastes Fructus Forsythiae, Flos Lonicerae decoct with water secondary, are respectively 1.5 hours, 1 hour.Cortex Phellodendri decocts with water secondary, each 2 hours.Collecting decoction, filtration, filtrate decompression are condensed into extractum.Rhizoma Coptidis, Radix Scutellariae powder are broken into fine powder, add mixing in the thick paste, and drying is pulverized, sieve, and granulation, tabletting, coating is promptly.
Embodiment five: the traditional method of learning from else's experience collection processing is concocted: Rhizoma Coptidis 12.8 weight portions, Radix Scutellariae 21.3 weight portions, Cortex Phellodendri 14.2 weight portions, Flos Lonicerae 25.2 weight portions, Fructus Forsythiae 26.5 weight portions.Above five tastes Fructus Forsythiae, Flos Lonicerae decoct with water secondary, are respectively 1.5 hours, 1 hour.Cortex Phellodendri decocts with water secondary, each 2 hours.Collecting decoction, filtration, filtrate decompression are condensed into extractum.Rhizoma Coptidis, Radix Scutellariae powder are broken into fine powder, add mixing in the thick paste, and drying is pulverized, sieve, and granulation, tabletting, coating is promptly.
Embodiment six: the traditional method of learning from else's experience collection processing is concocted: Rhizoma Coptidis 12.0 weight portions, Radix Scutellariae 22.0 weight portions, Cortex Phellodendri 14.0 weight portions, Flos Lonicerae 28.0 weight portions, Fructus Forsythiae 24.0 weight portions.Above five tastes Fructus Forsythiae, Flos Lonicerae decoct with water secondary, are respectively 1.5 hours, 1 hour.Cortex Phellodendri decocts with water secondary, each 2 hours.Collecting decoction, filtration, filtrate decompression are condensed into extractum.Rhizoma Coptidis, Radix Scutellariae powder are broken into fine powder, add mixing in the thick paste, and drying is pulverized, sieve, and granulation, tabletting, coating is promptly.
Character: this product is a coated tablet, removes to show sepia behind the coating; Bitter in the mouth.
Differentiate: (1) gets 5 of this product, removes coating, and porphyrize adds 75% ethanol 10ml, and supersound process 20 minutes filters, and filtrate is as need testing solution.Other gets baicalin, chlorogenic acid reference substance, adds 75% ethanol respectively and makes the solution that every 1ml contains 0.2mg, in contrast product solution.According to thin layer chromatography (appendix VIB of Chinese Pharmacopoeia version in 2000) test, draw each 2 μ l of above-mentioned three kinds of solution, put respectively on same polyamide film, be developing solvent with acetic acid, launch, take out, dry, put under the ultra-violet lamp (365nm) and inspect.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the fluorescence speckle of same color.
(2) get 5 of this product, remove coating, porphyrize adds methanol 10ml, and supersound process 20 minutes filters, and filtrate is as need testing solution.Other gets Fructus Forsythiae control medicinal material 0.5g, shines medical material solution in pairs with legal system.Test according to thin layer chromatography (appendix VIB of Chinese Pharmacopoeia version in 2005), draw each 5 μ l of above-mentioned two kinds of solution, putting in same Sodium Tvlose respectively is on the silica gel g thin-layer plate of adhesive, with chloroform-methanol (5:1) is developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing at 110 ℃.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the speckle of same color.
(3) get Rhizoma Coptidis control medicinal material 50mg, add methanol 5ml, supersound process 20 minutes filters, and filtrate adds methanol to 5ml, in contrast medical material solution.Get the berberine hydrochloride reference substance again, add methanol and make the solution that every 1ml contains 0.5mg, in contrast product solution.Test according to thin layer chromatography (appendix VIB of Chinese Pharmacopoeia version in 2005), draw and differentiate (2) item need testing solution and each 1 μ l of above-mentioned two kinds of contrast solutions down, put respectively on same silica gel g thin-layer plate, with benzene-ethyl acetate-methanol-isopropyl alcohol-ammonia solution (12:6:3:3:1) is developing solvent, put with in 15 minutes the expansion cylinder of ammonia steam presaturation, launch, take out, dry, put under the ultra-violet lamp (365nm) and inspect.In the test sample chromatograph, with control medicinal material chromatograph and the corresponding position of reference substance chromatograph on, show identical yellow fluorescence speckle.
Check: should meet every regulation relevant under the tablet item (appendix ID of Chinese Pharmacopoeia version in 2005) assay: measure according to high performance liquid chromatography (appendix VID of Chinese Pharmacopoeia version in 2005).
Differentiate: chromatographic condition and compatibility test octadecylsilane chemically bonded silica are filler: methanol-water-phosphoric acid (45:55:0.04) mobile phase: the detection wavelength is 276nm.Number of theoretical plate calculates by the baicalin peak should be not less than 5500.
The preparation of reference substance solution: precision takes by weighing at 4 hours baicalin reference substance of 60 ℃ of dryings an amount of, adds methanol and makes the solution that every 1ml contains 30 μ g, promptly.
The preparation of need testing solution: get 20 of this product, remove coating, the accurate title, decided porphyrize, get an amount of (being equivalent to 4 approximately), the accurate title, decide, and the accurate 45% methanol 50ml that adds claims to decide weight, reflux 2 hours is put coldly, claims to decide weight again, supplies the weight that subtracts mistake with 45% methanol, shake up, filter, discard filtrate just, precision is measured subsequent filtrate 1ml, put in the 25ml measuring bottle, add mobile phase, shake up to scale, filter with microporous filter membrane (0.45um), get subsequent filtrate, promptly.
Algoscopy: accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing, inject chromatograph of liquid, measure, promptly.
Every of this product contains Radix Scutellariae in baicalin (C21H18011), must not be less than 7.5mg.
Cure mainly and function: heat-clearing and toxic substances removing.Be used for the pathogenic wind-warm calentura, heating cough with asthma, aphtha pharyngeal swelling, diseases such as pyretic stranguria dysentery.
Usage and dosage: oral, three times on the one, be grown up one time 6～8; Child eight years old to 14 years old, one time 4～6; Four to seven years old, one time 3～4; One-year-old to three years old, 1～2.Not one year old children's is cut down according to the circumstance.
The property of medicine of the medicine of respectively distinguishing the flavor of in the present invention's prescription:
Rhizoma Coptidis is the dry rhizome of ranunculaceae plant Rhizoma Coptidis, Coptis deltoidea C.Y.Cheng et Hsiao or Coptis Teeta Wall.Hardship, cold.GUIXIN, spleen, stomach, liver, gallbladder, large intestine channel.Heat clearing and damp drying, eliminating fire and detoxication.Cure mainly damp and hot feeling of fullness, the vomiting acid regurgitation, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood is told nosebleed, conjunctival congestion, the carbuncle furuncle is quenched one's thirst in toothache; External treatment eczema, eczema, auditory meatus is suppurated.The kind clear part of the body cavity above the diaphragm housing the heart and lungs of Rhizoma Coptidis (processed with wine) is burning hot.Be used for conjunctival congestion, aphtha, prepared RHIZOMA COPTIDIS with rhizoma zingiberis recens juice clearing stomach stomach function regulating preventing or arresting vomiting.Be used for cold and heat and tie mutually, damp and hot middle resistance, feeling of fullness vomiting.Cornel Rhizoma Coptidis soothing liver-QI stomach function regulating preventing or arresting vomiting.Be used for incoordination between the liver and stomach, the vomiting acid regurgitation.
Cortex Phellodendri is the dry bark of rutaceae Cortex Phellodendri wampee.Bitter, cold.Return kidney, urinary bladder channel.Heat clearing away is scorching, and pathogenic fire purging removes steams, the detoxifcation furuncle.Be used for damp-heat dysentery, jaundice, leukorrhagia, pyretic stranguria, beriberi ， flaccidity of feet with lamenness, hectic fever due to YIN-deficiency consumptive fever, night sweat, seminal emission, sore swollen toxin, eczema Pruritus.The stir-baked CORTEX PHELLODENDRI with salt solution nourishing YIN to lower pathogenic fire.Be used for hyperactivity of fire caused by deficiency of YIN, the night sweat hectic fever due to YIN-deficiency.
Radix Scutellariae is the dry root of labiate Radix Scutellariae.Hardship, cold.Return lung, gallbladder, spleen, large intestine, small intestine meridian.Heat clearing and damp drying, eliminating fire and detoxication, hemostasis, antiabortive.Be used for hygropyrexia, the warm vomiting and nausea uncomfortable in chest of administration, damp and hot feeling of fullness, dysentery, jaundice, the cough due to lung-heat, the hyperpyrexia excessive thirst, heat in blood is told nosebleed, carbuncle sore tumefacting virus, frequent fetal movement.
Flos Lonicerae is caprifoliaceae plant dry flower or the first flower of opening of band of Radix Ophiopogonis.Sweet, cold.Return lung, the heart, stomach warp.Heat-clearing and toxic substances removing, wind-heat dissipating.Be used for the carbuncle furuncle, sore throat, erysipelas, toxic-heat and blood stasis, anemopyretic cold, epidemic febrile disease heating.
Fructus Forsythiae is the dry fruit of Oleaceae plant Fructus Forsythiae.Hardship is slightly cold.Return lung, the heart, small intestine meridian.Heat-clearing and toxic substances removing, dispersing swelling and dissipating binds.Be used for carbuncle, scrofula, acute mastitis, erysipelas,, anemopyretic cold, epidemic febrile disease is from the beginning of, hyperpyrexia excessive thirst, and coma is sent out speckle, the pyretic stranguria urine retention.
Pharmacological evaluation
The Sanhuang Qingjie sheet is to the laboratory observation of influenza poison and the influence of white mice phagocytic function
ShanXi Chinese Medicine Academy Chong Hong Sun Xu light Zhang Ying
The Sanhuang Qingjie sheet is a kind of antipyretic-detoxicant preparation of prescription, and is remarkable to the acute respiratory infection effect except that the various bacteria sexuality is had the certain curative effect through clinical observation, and acute respiratory infection kind 90-95% is caused by virus.So be worth further studying and inquiring into its curative effect mechanism; for clinical practice provides experimental basis; for this reason; we have carried out the resisiting influenza virus effect outside Embryo Gallus domesticus of Sanhuang Qingjie sheet; resisiting influenza virus effect in Embryo Gallus domesticus; to the experiment and the phagocyte experiment of the white mice protective effect of influenza virus infection, and to corresponding experiment result, be that index has been carried out laboratory observation to the variation of white mice immunologic function to the mortality rate of white mice protective effect and macrophage percentage rate and macrophage phagocytic index.Now, be reported as follows the preliminary experiment result.
Experiment material
1. Strain: Shanghai 74-5 influenzae strain virus is from Institute of Viruses of Academy of Medical Sciences, Beijing.After Embryo Gallus domesticus goes down to posterity, preserve standby.
Influenza virus A-prime FM1 Mus lung adapts to available from Chinese Academy of Medical Sciences institute of antibiotics.
2. medicinal liquid: by the Xi'an Sanhuang Qingjie sheet ethanol extract that the pharmaceutical factory of traditional Chinese medicine provides of relying on oneself, every milliliter contains crude drug 0.5 gram.
3. Embryo Gallus domesticus: adopt order on the 10th to enjoy kind of an Embryo Gallus domesticus.
4. laboratory animal: the Kunming white mouse that adopts four army large animal rooms to provide, body weight are respectively 12-14 gram 18-20 gram, health, male and female half and half.
5. starch flour meat soup liquid: starch 5 grams, flour 3 grams, 100 milliliters of adding distil waters, mixing boils into starchiness, 5 milliliters of every pipe packing, 8 pounds of sterilizations in 20 minutes, refrigerator is preserved standby, and the time spent adds row injection again behind the aseptic meat soup mixing of equivalent.
6. chicken erythrocyte suspension preparation, enjoy chicken wing vein with asepsis injector from health and get blood, mix in the A Shi liquid of 5 times of blood volumes, put in 4 degrees centigrade of refrigerators and preserve, use physiological saline solution centrifuge washing three times before using, be made into 5% normal saline chicken erythrocyte suspension.
7. diluent: be freshly prepared PH7.0 buffer saline.
Experimental technique and result
1. the experiment of the anti-Shanghai of Sanhuang Qingjie sheet 74-5 influenzae strain toxic action.
(1) experiment of Sanhuang Qingjie sheet anti-Shanghai 74-5 influenzae strain virus function outside Embryo Gallus domesticus: Sanhuang Qingjie sheet ethanol extract is begun with buffer saline 1:40, doubly amount is diluted to 1:1280, and respectively with the viral carbamide liquid mixed in equal amounts (matched group replaces medicinal liquid with slow group saline) of 0.1 HAU.After when placing 37 degrees centigrade of water-bath effects 1, each dilution factor is inoculated in 4 hatches in the Embryo Gallus domesticus carbamide that made in 10th, every is injected 0.3 milliliter, in 35 degrees centigrade of incubators, hatched 48 hours, collect live chickens embryo carbamide liquid and carry out the blood clotting experiment, blood clotting phenomenon person does not appear in the whole Embryo Gallus domesticus carbamide liquid that contain high dilution medicinal liquid, then judges this concentration for to tiring that this influenza virus is worked, and the result is as shown in table 1:
The experimental result of table 1. Sanhuang Qingjie sheet anti-Shanghai 74-5 influenzae strain virus function outside Embryo Gallus domesticus
The drug level result
1：40 0/4
1：80 0/4
1：160 0/4
1：320 0/4
1：640 0/4
1：1280 0/4
Virus control group 4/4
Annotate: divide the subrepresentation blood clotting positive embryo number, denominator is represented the embryo number.
Table 1 shows: the Sanhuang Qingjie sheet has the effect of resisiting influenza virus outside embryo, and it tires 〉=experiment of 1280 (2) Sanhuang Qingjie sheets anti-Shanghai 74-5 influenzae strain virus function in Embryo Gallus domesticus:
Sanhuang Qingjie sheet alcohol extract is begun doubly amount dilution 1:32 and takes mixed liquor with the viruria of 0.1 HAU respectively with buffer saline 22:2.Three Embryo Gallus domesticus of each dilution factor injection of each medicinal liquid through 35 degrees centigrade of 48 hours hatch, are collected live chickens embryo carbamide liquid and are carried out the blood clotting experiment, and the wing judges that the susceptible toxic action of this medicine convection current tires.The result is as shown in table 2.
The experimental result of table 2. Sanhuang Qingjie sheet anti-Shanghai 74-5 influenzae strain virus function in Embryo Gallus domesticus.
The drug level experimental result
1:2 0/3
1:4 0/3
1:8 0/3
1:16 3/3
1:32 3/3
Virus control group 3/3
Annotate: divide the positive embryo of subrepresentation blood clotting to loose, denominator represents to test the embryo number
From table again as can be seen after, the Sanhuang Qingjie sheet to Shanghai 74-5 influenzae strain virus infect administration simultaneously can the demonstration effect.
3. the Sanhuang Qingjie sheet is to the laboratory observation of the white mice protective effect of influenza virus infection:
Adopt animal housing of The Fourth Military Medical University that Kunming white mouse is provided, body weight 12-14 gram, healthy male and female half and half.White mice given immediately be administration group and matched group, 15 every group.Administration group lumbar injection Sanhuang Qingjie sheet 0.12-0.14ml/ time (LD50 amount 21.26+1.58g/kg body weight), matched group white mice inoculation 0.12-0.14ml sterile saline.Slightly liquor-saturated down after 24 hours at ether, give every experimental group and matched group white mice each intranasal inoculation 10-4 virus 0.03ml (trying to achieve) for this virus LD50 measures, by the administration respectively of intraperitoneal administration approach, give normal saline 3 days again.Meanwhile examine day by day to the 14th day, should notice during observation that white mice has or not lethargy, Folium Pini, the generation of the phenomenon of symptoms such as dyspnea and death, self-infection virus two groups of mice dying numbers of elements of 36 hours opening entries (dead person disregards in 36 hours), according to two groups of mice dying rates, result of determination.The result is as shown in table 3.
Table 3. Sanhuang Qingjie sheet is to the protective effect of influenza virus infection white mice:
Table 3 shows that the Sanhuang Qingjie sheet has certain protective role to the influenza virus infection white mice, with the matched group contrast, notable difference (P＜0.01) is arranged.
The experiment of Sanhuang Qingjie sheet antiinflammatory action
(1) experimental technique and result:
1. the laboratory observation of anti-Oleum Tiglii induced mice auricular concha inflammation:
(1) animal: adult mice, body weight 18-22 gram, male and female are not limit, and the Xi'an is defended animal field, school and is provided.
(2) medicine: 2% Oleum Tiglii; Pure Oleum Tiglii is provided by the Xi'an Chinese medicine of relying on oneself, and is diluted to 2% concentration with vegetable oil, and the storage refrigerator is standby.
0.5% cetacort: the Shanghai Xinyi Pharmaceutical Factory is produced.
(3) experimental technique:
1. mice is divided into after only weighing at random: experimental group (B), medicine matched group (H) and saline control group (N) are raised for three groups under the same conditions.
2. following medicine is injected in each treated animal skin chamber, once a day, and continuous eight days;
B group: every animal skin chamber injection Sanhuang Qingjie sheet 15ml/ kg body weight, (containing crude drug 7.5 grams)
The H group: 5% cetacort 10ml/ kg body weight is injected in every animal skin chamber, (containing 50 milligrams of cetacorts)
The N group:: every animal skin chamber injection physiological saline solution 10ml/.
3. administration is the 8th day, after administration 30 minutes, be coated with 2% Oleum Tiglii 0.07ml and respectively organize mice left side auricular concha, all put to death after four hours, with the 5mm card punch mice left and right sides auricular concha is taken off respectively, on balance, take by weighing weight respectively, and, between each group, compare and calculate the inflammation suppression ratio with following formula by only calculating two weight differences:
Suppression ratio=(matched group two ear weight difference-experimental grouies two ear weight differences)/matched group two ear weight difference * 100%
2. experimental result sees Table:
Experiment is respectively organized mice two ear weight differences relatively
3. median lethal dose(LD 50) is measured according to simplifying probit and is sent out a skin chamber injection median lethal dose(LD 50) of Sanhuang Qingjie sheet mice (LD50) of survey and be: 21.26+1.0.2 gram * 12 * 2 plates 58 gram/kg body weight.
(2) brief summary and discussion:
Report according to clinical data: Chinese medicine Sanhuang Qingjie sheet all has significant clinical efficacy to the inflammation due to the Different types of etiopathogenises, according to these characteristics, adopted with the Oleum Tiglii local excitation and made the observation that experimentizes of nonspecific animal model, in the hope of make zoopery as far as possible near meeting clinical practice.
Observe in the experiment of anti-Oleum Tiglii induced mice auricular concha inflammation, the inflammation suppression ratio of Sanhuang Qingjie sheet and hydrocortisone is all more than 60%, compares with the normal saline matched group for two groups, and highly significant difference (P＜0.001) is all arranged.Inferring the evil antiinflammatory action of Sanhuang Qingjie sheet, may be by the vascular reaction in certain machine-processed inflammation-inhibiting process, and the minimizing inflammation realizes oozing out of liquid.
Some cases are provided:
(1) Bai Ke, the man, 7 years old, head of a family's text of an annotated book Ji, warehouse, Shi Jia street, first visit fever half a day, companioned with headache, dry pharynx, pharyngalgia, hoarseness, two are just normal.Body temperature is 38 ℃, and mind is clear, swallows red, both sides antiadoncus, red tongue, white and thin fur, floating and rapid pulse, hemogram chemical examination, leukocyte 10200, neutrality 69%, lymph 31%.
Card belongs to: flu (anemopyretic)
Prescription: 36 of Sanhuang Qingjie sheets, each 3, four times a day.
Vitamin C 0.16g, every day three times.
Curative effect: after being ill with the side, the sick improvement after its mother tells and took medicine two days, recovery from illness after three days.
(2) about the answer of Sanhuang Qingjie sheet trial effect
Li Zheng, having a fever is, 38.4 ℃ of body temperature are seen outpatient service to city hospital, are diagnosed as " acute tonsillitis " through doctor Du.Medicine " Sanhuang Qingjie sheet " on probation was promptly brought down a fever later in one day, and blood test is normal after three days.
The Li Zheng cough to this institute, please doctor Du check that " Sanhuang Qingjie sheet " used in diagnosis " tonsillitis " again, medicine totally four days, check is normal.
Li Zheng to accommodate tonsillitis several times, is to treat with penicillin.This twice usefulness Sanhuang Qingjie sheet treatment, on treatment time and effect we think suitable with penicillin, and the misery that child has been exempted have an injection and a lot of troubles of the head of a family.
Claims (2)
1. a detoxifcation heat clearing Chinese medicine that is used for the treatment of internal medicine excess-fire card class is characterized in that: made by following raw material medicaments: Rhizoma Coptidis 12-15 weight portions, Radix Scutellariae 18-24 weight portions, Cortex Phellodendri 11-16 weight portions, Flos Lonicerae 24-29 weight portions, Fructus Forsythiae 19-27 weight portions.
2. a kind of manufacture method that is used for the treatment of the detoxifcation heat clearing Chinese medicine of internal medicine excess-fire card class according to claim 1, it is characterized in that: processing step is: Fructus Forsythiae, Flos Lonicerae decoct with water secondary, are respectively 1.5 hours, 1 hour; Cortex Phellodendri decocts with water secondary, each 2 hours; Collecting decoction, filtration, filtrate decompression are condensed into extractum; Rhizoma Coptidis, Radix Scutellariae powder are broken into fine powder, add mixing in the thick paste, and drying is pulverized, sieve, and granulation, tabletting, coating is promptly.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108567864A (en) * | 2016-04-01 | 2018-09-25 | 重庆市畜牧科学院 | Chinese medicine preparation for treating sore mouth virus |
| CN110157667A (en) * | 2019-06-06 | 2019-08-23 | 广州鸿泉生物科技有限公司 | A kind of preparation method of mouse red blood cell suspension |
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2008
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108567864A (en) * | 2016-04-01 | 2018-09-25 | 重庆市畜牧科学院 | Chinese medicine preparation for treating sore mouth virus |
| CN110157667A (en) * | 2019-06-06 | 2019-08-23 | 广州鸿泉生物科技有限公司 | A kind of preparation method of mouse red blood cell suspension |
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