CN101352156B - Method for hatching Changbaishan female wood frog - Google Patents

Abstract

The invention relates to a method for hatching female frogs of Changbaishan forest frogs, comprising the following steps: a closed hatching sieve is made and hatching is carried out: firstly, the hatching sieve is put into a hatching pool, the water depth of which is 0.6 to 3 meters, then the collected egg masses are put into the hatching sieve which is shaded, the temperature in the sieve is kept between 5 DEG C and 25 DEG C, the collected masses in a same sieve can not exceed three days, after fertilized eggs are hatched into polliwogs and the egg jelly is eaten up, the sieve is taken away to lead the polliwogs to run freely in the hatching pool; a feeding table and a shading shed is arranged; feeding; animal fat is cut into particles, roasted and uniformly mixed with food stuffs for forest frogs and polliwogs, the fat content is between 5 percent to 7 percent, feeding is carried out twice a day, and the food stuffs for frogs cannot contain estrogen. By adopting the method, more than 70 percent of the forest frogs are female individuals. Besides, the method has no hidden troubles to descendant individuals and can increase the output of forest frog oil.

Description

Method for hatching Changbaishan female wood frog

Technical field

The present invention relates to a kind of three provinces in the northeast of China Changbai Mountain wood frog envelope ditch and culture female hatching technique.

Background technology

In envelope ditch breed of current three provinces northeastward and the intensive culture wood frog industry, the female-male proportion detuning phenomena is arranged, male accounting for about 70%, female accounting for about 30%, and wood frog oil is female oviduct, female ratio directly influences the output of China's wood frog oil less, and the female hatching of three provinces in the northeast of China's forest frog culture industry at present has two kinds of methods: the one, and hormone method, the 2nd, low temperature hatching method.

The hormone method is " the existing introduction in wood frog industry knowledge handbook one book, this book has been introduced the zooscopy person Zhang Zhiyi of the Chinese Academy of Sciences and Yao of academy of agricultural sciences, Jilin Province and unit's cooperation, project verification in 1987 Changbai Mountain wood frog sex controlling has been carried out the research in 6 years by a definite date, last conclusion is: use 17B-estradiol effect optimum, optium concentration is that every premium on currency uses 25---100ug, concentration surpasses 150ug/L, to produce male and male and female binary individuality, sex hormone induces sex can only use (property in spring processing then is to catching autumn then) in the commodity frog produces.Mr. Zhang Zhiyi proposes theoretically, and false female through hormone-treated female genus still, false female wood frog and public frog mating might produce whole males.

The low temperature hatching method has prolonged the growth of tadpole, abnormal time, and feeding cost increases, and the rate of recapture descends, and water temperature is difficult to control in addition, so nobody adopts in foster frog producer.

Summary of the invention

The purpose of this invention is to provide a kind of forest frog tadpoles of control naturally to female sexual development and to the Changbaishan female wood frog frog hatching m of offspring without any hidden danger.

Purpose of the present invention realizes by following measure:

A kind of method for hatching Changbaishan female wood frog comprises the steps:

A, the closed hatching sieve of manufacturing, hatching

Spring wood frog go out before the river lays eggs, be ready to the hatching sieve, make framework with plank all around, the bottom is provided with gauze and constitutes the hatching sieve, to hatch sieve and put into the hatchery, the degree of depth of hatchery water is between 0.6-3 rice, then the ovum group of collecting is put into the hatching sieve, in the above with branch put up arch be fixed on around on the plank, repaved black plastic cloth on branch, fixed, temperature remains on 5 ℃-25 ℃ in the sieve, ovum group in the same sieve must not be above three days, after treating that incubating oosperm becomes tadpole to eat up egg jelly, remove sieve, allow tadpole be active in the hatchery voluntarily;

B, foodstuff platform and shade are set

Before removing the hatching sieve, to build the tadpole foodstuff platform around the hatchery, foodstuff platform is placed the depth of water about 20 centimetres, then above the tadpole foodstuff platform 1 meter highly vertically set up parallel bracket, width is identical with foodstuff platform, repave black spun yarn net above or black plastic cloth is fixed, tadpole diet under the environment that shelters from heat or light is grown;

C, feeding

Animal tallow is cut into particle, fries and do, stir with the forest frog tadpoles foodstuff then, fat content accounts between the 5%-7% of foodstuff weight, throws something and feeds twice every day, mustn't contain oestrogenic hormone in the tadpole foodstuff.

The Changbai Mountain wood frog does not have sex chromosome, does not have genitalia, and it is in vitro fertilization, ectogenetic amphibian animal.Signal in the egg mother cell matter is arranged in male and female differentiation, be subjected in the nature again simultaneously, the conduction of water temperature illumination.Changbai Mountain wood frog male and female differentiation is not influenced by year human relations, and the inventor experiences the theory study and the experiment in annual spring in 4 years, has found the rule of male and female differentiation.By the present invention program, forest frog tadpoles artificially is set to the space environment of female sexual development and to the needed enough material energy of female sexual development, can reach the female individuals that contains in the wood frog colony more than 70%, and to offspring's individuality without any hidden danger.The present invention has looked for the basic reason of nature control forest frog tadpoles male and female differentiation, therefore can be enough to make the double growth of China's wood frog oil yield.

Embodiment

A kind of Changbaishan female wood frog frog hatching m comprises the steps:

A, the closed hatching sieve of manufacturing, hatching

Spring wood frog go out before the river lays eggs, be ready to the hatching sieve, make framework with plank all around, the bottom is provided with gauze and constitutes the hatching sieve, the specification of hatching sieve is long 5 meters, wide 1-2 rice, high 20 centimetres cuboid, to hatch sieve and put into the hatchery, the degree of depth of hatchery water is put into the ovum group of collecting the hatching sieve then between 0.6-3 rice, in the above with branch put up arch be fixed on around on the plank, on branch, repaved black plastic cloth, fixed, prevented to be blown away, temperature remains on 5 ℃-25 ℃ in the sieve, temperature is too high, and to take Polypropylence Sheet off aeration-cooling, and the ovum group in the same sieve must not surpass three days, treat that incubating oosperm becomes tadpole to eat up egg jelly after, remove sieve, allow tadpole be active in the hatchery voluntarily; Is 7 days from oocyte fertilization to hatching into tadpole;

B, foodstuff platform and shade are set

Before removing the hatching sieve, periphery around in the hatchery will be built the tadpole foodstuff platform, and the specification of foodstuff platform is wide about 1 meter, about 20 centimetres of height, place the depth of water 20 centimetres of (apart from horizontal plane) positions, the left and right sides, be preferably in 20 centimetres, 1 meter height above the tadpole foodstuff platform is vertically set up parallel bracket then, width is identical with foodstuff platform, repave black spun yarn net above or black plastic cloth is fixed, prevent to be blown away, tadpole diet under the environment that shelters from heat or light is grown;

C, feeding

Animal tallow is cut into particle, fry does, stir with the forest frog tadpoles foodstuff then, fat content accounts between the 5%-7% of foodstuff weight, throws something and feeds twice every day, and about 8 of mornings, once mid-afternoon once mustn't contain oestrogenic hormone in the tadpole foodstuff.From the tadpole to the abnormal young frog, need 50 days.

The inventor is according to the experiment experience of theoretical direction and 4 years, at in April, 2008 branch, carry out the 32nd experiment of female wood frog hatching in Feng Gou cultivation base, Jilin Province's Dunhua State Forestry Administration, P.R. China big stone river forest farm, utilize technical solution of the present invention, behind the abnormal formation of the forest frog tadpoles froglet, 64 wood frog sexual glands have arbitrarily been extracted, sexual gland is made section, 46 ovum histocytes under microscopic examination, have been obtained, determined to develop into 70% female individuals by drawing, because adopt the place control mode, thus to offspring's individuality without any hidden danger.

Claims (2)

1. a method for hatching Changbaishan female wood frog comprises the steps:

A, the closed hatching sieve of manufacturing, hatching

Spring wood frog go out before the river lays eggs, be ready to the hatching sieve, make framework with plank all around, the bottom is provided with gauze and constitutes the hatching sieve, to hatch sieve and put into the hatchery, the degree of depth of hatchery water is between 0.6-3 rice, then the ovum group of collecting is put into the hatching sieve, in the above with branch put up arch be fixed on around on the plank, repaved black plastic cloth on branch, fixed, temperature remains on 5 ℃-25 ℃ in the sieve, ovum group in the same sieve must not be above three days, after treating that incubating oosperm becomes tadpole to eat up egg jelly, remove sieve, allow tadpole be active in the hatchery voluntarily;

B, foodstuff platform and shade are set

Before removing the hatching sieve, to build the tadpole foodstuff platform around the hatchery, foodstuff platform is placed the depth of water about 20 centimetres, then above the tadpole foodstuff platform 1 meter highly vertically set up parallel bracket, width is identical with foodstuff platform, repave black spun yarn net above or black plastic cloth is fixed, tadpole diet under the environment that shelters from heat or light is grown;

C, feeding

Animal tallow is cut into particle, fries and do, stir with the forest frog tadpoles foodstuff then, fat content accounts between the 5%-7% of foodstuff weight, throws something and feeds twice every day, mustn't contain oestrogenic hormone in the tadpole foodstuff.

2. a kind of method for hatching Changbaishan female wood frog according to claim 1 is characterized in that the specification of described hatching sieve is long 5 meters, wide 1-2 rice, high 20 centimetres cuboid.