CN101322729B - Using method of Chinese chestnut kernel alcohol extract as antiinflammatory medicament - Google Patents
Using method of Chinese chestnut kernel alcohol extract as antiinflammatory medicament Download PDFInfo
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- CN101322729B CN101322729B CN2008101348223A CN200810134822A CN101322729B CN 101322729 B CN101322729 B CN 101322729B CN 2008101348223 A CN2008101348223 A CN 2008101348223A CN 200810134822 A CN200810134822 A CN 200810134822A CN 101322729 B CN101322729 B CN 101322729B
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Abstract
The invention relates to an application method of taking Chinese chestnut kernel alcohol extract as anti-inflammatory drug. The application method of the extractum of taking the Chinese chestnut kernel alcohol extract as the anti-inflammatory drug comprises: a. oral administration: the extractum of the chestnut kernel alcohol extract is made into solid drug for direct oral administration; b. injection: the extractum of the Chinese chestnut kernel alcohol extract is placed in a mortar, added with Tween 80, ground thoroughly, and added with physiological saline for a plurality of times while grinding until the extract is ground evenly; the mixture is placed in an injector and taken as injection drug. The core raw material in the invention is the Chinese chestnut kernel which is produced in Qianxi County, Hebei Province, has abundant resources, wide sources, and high medicinal value. The application method of the extractum of the Chinese chestnut kernel alcohol extract in the anti-inflammatory drugs is simple, and animal experiments show that the Chinese chestnut kernel alcohol extract has obvious anti-inflammatory effect.
Description
Technical field
The present invention relates to Chinese chestnut kernel the extract application process in medicine, especially Chinese chestnut kernel alcohol extract method for using as anti-inflammatory drug.
Background technology
Semen Castaneae (Castanea mollissima Blume) is China's special product, have the good reputation of " king of dry fruit ", and it also is called as " Herba Herminii " abroad.Motherland's medical science thinks that Semen Castaneae is warm in nature, and sweet in the mouth is flat, goes into spleen, stomach, kidney channel, and effects such as the kidney invigorating, spleen invigorating are arranged.About the medical value of Semen Castaneae, the traditional Chinese medical science thinks that the chestnut fruit has nourishing stomach and spleen, the strong muscle of the kidney invigorating, and the function of promoting blood circulation and hemostasis, and be of value to hypertension, the sick control of hat.Diseases such as clinically, Semen Castaneae also can be used for treating regurgitation, has loose bowels, the waist lower limb is weak, spit blood, have blood in stool, incised wound.Simultaneously, Semen Castaneae adds that other Chinese medicines or food material can process medicated diet, the treatment tracheitis, suffers from a deficiency of the kidney diseases such as dyspepsia, diarrhoea, apoplexy.
Chinese patent " extractive of chestnut kernel and preparation method thereof and the application " (publication number is CN101129426A) in promoting the gastrointestinal peristalsis medicine; The application of kernel of Semen Castaneae Mollissimae in promoting the gastrointestinal peristalsis medicine just disclosed; Up to now, the record that kernel of Semen Castaneae Mollissimae is used in anti-inflammatory drug does not also appear.
Summary of the invention
Goal of the invention of the present invention is to Chinese chestnut kernel extract range of application narrow in medicine the method for using of a kind of Chinese chestnut kernel alcohol extract as anti-inflammatory drug to be provided.
Realize the foregoing invention purpose, adopt following technical scheme: a kind of Chinese chestnut kernel alcohol extract is raw material as the method for using of anti-inflammatory drug with the Chinese chestnut kernel, and its method for distilling is: (1) is ground into coarse powder with the Semen Castaneae peeling, sieves; (2) with 75% alcohol heating reflux of 10 times of amounts 2 times, each 1.5 hours, decompression recycling ethanol was to there being the ethanol flavor, Chinese chestnut kernel alcohol extract extractum, with its method for using be as anti-inflammatory drug:
A, oral: Chinese chestnut kernel alcohol extract extractum is prepared into solid drugs, directly oral;
B, injection: Chinese chestnut kernel alcohol extract extractum is placed mortar, add tween 80 and fully grind, divide to add for several times normal saline again, edged mill in limit till fully grinding evenly, is placed in the syringe, uses as injectable drug.
Core material-Chinese chestnut kernel of being mentioned among the present invention, the Semen Castaneae of adopting the Qianxi County, Hebei province to produce, aboundresources, wide material sources, medical value is high; And the application process of the extractum of Chinese chestnut kernel ethanol extraction in anti-inflammatory drug is simple, shows that through the zoopery result Chinese chestnut kernel alcohol extraction thing has the drug action of tangible antiinflammatory pharmacology.
The specific embodiment
Below in conjunction with embodiment the present invention is detailed.
The method for distilling of the Chinese chestnut kernel alcohol extract described in the present invention, its step is following:
(1) with the Semen Castaneae peeling, is ground into coarse powder, crosses 14 mesh sieves;
(2) with 75% alcohol heating reflux of 10 times of amounts 2 times, each 1.5 hours, decompression recycling ethanol was to there being the ethanol flavor, pure extractum;
The Semen Castaneae coarse powder can be the 10-40 order in the above-mentioned steps (1); Used concentration of alcohol is 60-95% in the step (2), and amount of ethanol is 8-15 times, and method for distilling is percolation or reflux.Percolation in the above-mentioned steps be whole ethanol percolation liquid percolation are finished till; The heating and refluxing extraction method is to extract 3 times, each boiling reflux 1 hour to 2 hours.
The Chinese chestnut kernel ethanol extraction is tested with white mice as the method for using of anti-inflammatory drug, and operational approach and experiment effect are following:
(1) Chinese chestnut kernel ethanol extraction xylol causes the inhibitory action experiment of mice ear:
1. experiment material
1.1 sample, Chinese chestnut kernel alcohol extraction thing extractum, face the time spent to be mixed with solution, compound method: it is an amount of to get extractum; High dose group is got the about 120mg of extractum, and low dose group is got the about 60mg of extractum, and accurate the title decides; Put in the mortar, add the 0.1ml tween 80, fully grind; Get the 10ml normal saline again and divide adding for several times, the limit edged grinds, till fully grinding evenly.Positive control drug: aspirin (beach, Shenzhen pharmaceutical factory)
1.2 reagent, xylene (Shenyang chemical reagent factory).
1.3 instrument: electronic balance (SartoriousBP211D), card punch.
1.4 animal: Kunming mouse, male and female half and half 18-22g, but there is Shenyang medicine university animal center to provide.
2, method and result
90 of male mice in kunming are divided into 9 groups at random, 10 every group.3 kinds of given the test agent are established low dose group 0.15g/kg and high dose group 0.30g/kg and normal saline, tween 80-normal saline, positive control aspirin totally 9 experimental grouies respectively.Each organizes gastric infusion, for three days on end, and behind last administration 2h (aspirin administration 1h); Xylene 0.05mL is applied to the mouse right ear tow sides, and cervical vertebra dislocation is put to death behind the 15min, with diameter 9mm card punch about the punching of two auricle same area take a sample; In time weigh; Be calculated as follows suppression ratio and swelling degree, carry out statistical procedures with the t check, and compare with matched group.
Suppression ratio=(matched group swelling degree-administration group swelling degree) ÷ matched group swelling degree * 100% swelling rate=(auris dextra sheet heavy-left auricle is heavy) ÷ left side auricle is heavy
Table 1 Chinese chestnut kernel ethanol extraction xylol causes the antiinflammatory action of mice ear
* compare P＜0.05 with tween 280-normal saline group;
* and normal saline group compare, P＜0.05.
Experimental result shows: Chinese chestnut kernel ethanol extraction 0.30g/kg dose groups xylol causes mice ear has obvious inhibitory action, and suppression ratio is respectively 35.81% and 42.14%, and respectively with negative control group 2, group 1 is than there were significant differences (P＜0105).
(2) the Chinese chestnut kernel ethanol extraction is tested the influence of mouse peritoneal capillary permeability:
1. experiment material
1.1 sample, Chinese chestnut kernel alcohol extraction thing extractum, face the time spent to be mixed with solution, compound method: it is an amount of to get extractum; High dose group is got the about 120mg of extractum, and low dose group is got the about 60mg of extractum, and accurate the title decides; Put in the mortar, add the 0.1ml tween 80, fully grind; Get the 10ml normal saline again and divide adding for several times, the limit edged grinds, till fully grinding evenly.Positive control drug: aspirin (beach, Shenzhen pharmaceutical factory)
1.2 reagent, Yi Wensilan, glacial acetic acid (Shenyang chemical reagent factory)
1.3 instrument: electronic balance (SartoriousBP211D)
1.4 animal: Kunming mouse, male and female half and half 18-22g is provided by Shenyang Pharmaceutical University's animal center.
2, method and result
70 Kunming mouses are divided into 7 groups by the sex difference, 10 every group, male and female half and half; The administration of dividing into groups as stated above, each is organized behind last administration 2h (aspirin administration 30min), tail vein injection 1% Yi Wensilan (40mg/kg); While lumbar injection 0.7% glacial acetic acid 0.2mL, mice is put to death in the cervical vertebra dislocation behind the 15min, and injects the 5mL normal saline in mouse peritoneal; Extract peritoneal fluid in 4 ℃ of placement 6h, get its supernatant, with 751 spectrophotometers (590nm) colorimetric; Relatively the difference between administration group and matched group is calculated as follows suppression ratio, carries out statistics t check.
Suppression ratio=(matched group Yi Wensilan seepage discharge-administration group Yi Wensilan seepage discharge) ÷ matched group Yi Wensilan seepage discharge * 100%.
Table 2 Chinese chestnut kernel ethanol extraction is to the influence of mouse peritoneal capillary permeability
* compare P＜0.05 with tween 280-normal saline group.
Experimental result shows: Chinese chestnut kernel ethanol extraction 0.15g/kg dose groups has the effect (P＜0.05) of remarkable inhibition capillary permeability.
Infer that through above instance Chinese chestnut kernel ethanol extraction extractum also has significant antiinflammatory action to human body.
It is prepared into granular medicament or pill, and administering mode is an oral administration, calculates through body surface area, infers that the human body dosage is 1.16～2.33g/ man day (perhaps 0.39～0.78g/ time, every day 3 times).
Chinese chestnut kernel alcohol extract extractum is placed mortar, and high dose group is got the about 120mg of extractum, and low dose group is got the about 60mg of extractum; Adding tween 80 according to the need dose fully grinds; Divide to add normal saline for several times, edged mill in limit is till fully grinding evenly; Be placed in syringe or the injection, use as injectable drug.
Claims (1)
1. the application of Chinese chestnut kernel alcohol extract in the preparation anti-inflammatory drug is raw material with the Chinese chestnut kernel, and its method for distilling is: (1) is ground into coarse powder with the Semen Castaneae peeling, sieves; (2) with 75% alcohol heating reflux of 10 times of amounts 2 times, each 1.5 hours, decompression recycling ethanol was to there being the ethanol flavor, Chinese chestnut kernel alcohol extract extractum, it is characterized in that, with its application process be as anti-inflammatory drug:
A, oral: Chinese chestnut kernel alcohol extract extractum is prepared into solid drugs, directly oral;
B, injection: Chinese chestnut kernel alcohol extract extractum is placed mortar, add tween 80 and fully grind, divide to add for several times normal saline again, edged mill in limit till fully grinding evenly, is placed in the syringe, uses as injectable drug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101348223A CN101322729B (en) | 2008-08-01 | 2008-08-01 | Using method of Chinese chestnut kernel alcohol extract as antiinflammatory medicament |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101348223A CN101322729B (en) | 2008-08-01 | 2008-08-01 | Using method of Chinese chestnut kernel alcohol extract as antiinflammatory medicament |
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| Publication Number | Publication Date |
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| CN101322729A CN101322729A (en) | 2008-12-17 |
| CN101322729B true CN101322729B (en) | 2012-02-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2008101348223A Expired - Fee Related CN101322729B (en) | 2008-08-01 | 2008-08-01 | Using method of Chinese chestnut kernel alcohol extract as antiinflammatory medicament |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101129426A (en) * | 2007-08-17 | 2008-02-27 | 赵国强 | Extractive of chestnut kernel, method of preparing the same and application of the same in accelerating peristalsis of stomach and intestine |
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2008
- 2008-08-01 CN CN2008101348223A patent/CN101322729B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101129426A (en) * | 2007-08-17 | 2008-02-27 | 赵国强 | Extractive of chestnut kernel, method of preparing the same and application of the same in accelerating peristalsis of stomach and intestine |
Non-Patent Citations (3)
| Title |
|---|
| Dongsong Zhang et al.Flavonol glycosides from Castanea mollissima Blume.Asian Journal of Traditional Medicines.2007,第2卷(第5期),229-234. * |
| 许振朝.福星栗胶囊的药理作用研究.《中草药》.1997,第29卷(第1期),29-31. * |
| 金在久等.板栗的化学成分及药理研究进展.《山西医药杂志》.2005,第34卷(第9期),751-753. * |
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| CN101322729A (en) | 2008-12-17 |
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Granted publication date: 20120208 Termination date: 20120801 |