CN101249131B - Gynostemma pentaphylla effective ingredient extract - Google Patents
Gynostemma pentaphylla effective ingredient extract Download PDFInfo
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Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a Gynostemma pentaphyllum Makino soft capsule preparation, a preparation method thereof, an effective part extract of the Gynostemma pentaphyllum Makino and the preparation method thereof. The effective part extract of the Gynostemma pentaphyllum Makino, which contains Gynosaponin, flavone and amylase, is extracted and made from the Gynostemma pentaphyllum Makino through the ultrasonic water extraction and ethanol precipitation or the alcohol reflux ultrasonic water extraction method. The effective part extract of the Gynostemma pentaphyllum Makino is added into solvent for dissolving, the temperature of the solvent ranges from 50 to 80 DEG C; then auxiliary material such as stabilizing agent, etc., is added, and the solution is fully and evenly mixed in a spiral mixer; the solution which passes through a colloid mill is processed through desaeration, pelleting, finalization dryness, disk-arraying dryness, pill selection and polishing, so as to prepare the Gynostemma pentaphyllum Makino soft capsule preparation. The soft capsule preparation has the advantages that the dosage is small and easy to take, the effect is fast, the efficacy is definite, and has the functions of nourishing the heart, invigorating the spleen, supplementing Qi and activating blood circulation, expelling phlegm, absorbing clot, reducing blood lipid, antioxidation, enhancing body immunity, and improving the cardiovascular system.
Description
Technical field
The present invention relates to a kind of the effective-part of Gynostemma pentaphyllum extract and preparation method thereof, belong to medical technical field.
Background technology
Herb Gynostemmae Pentaphylli [Gynostemma pentaphyllum (thunb) mak] is a cucurbitaceae genus gynostemma.Herb Gynostemmae Pentaphylli contains multiple gypenoside to the human body beneficial, flavone, vitamin, aminoacid and polysaccharide.Studies show that the Herb Gynostemmae Pentaphylli active ingredient has effects such as blood fat reducing, blood sugar lowering, antitumor, defying age, resisting fatigue, the liver protecting and enhancing body immune function.Diseases such as Cardiovascular system disorders such as hyperlipemia, hypertension, coronary heart disease, diabetes, tumor had good prevention effect.Long-term edible having no side effect is described as China's " southern Radix Ginseng ", " anticancer rising star ".Motherland's medical science is thought, Herb Gynostemmae Pentaphylli bitter in the mouth, cold in nature, nontoxic.Eliminating inflammation and expelling toxin, the eliminating phlegm and stopping cough of being used among the people, the producing region masses adopt its stem and leaf in summer and fry in shallow oil water and make refreshment drink more.
Effective ingredient for the most effective extraction and raising Herb Gynostemmae Pentaphylli guarantees making full use of of Herb Gynostemmae Pentaphylli, and the increase drug effect reduces cost and increases economic efficiency, and the present invention overcomes the prior art deficiency, and a kind of the effective-part of Gynostemma pentaphyllum extract and preparation method thereof is provided.
Summary of the invention
One of purpose of the present invention provides a kind of the effective-part of Gynostemma pentaphyllum extract; Two of the object of the invention provides a kind of the effective-part of Gynostemma pentaphyllum preparation method of extract; It is the soft capsule preparation of primary raw material with the Herb Gynostemmae Pentaphylli effective part extract that three of the object of the invention provides a kind of; It is the preparation method of the soft capsule preparation of primary raw material with the Herb Gynostemmae Pentaphylli effective part extract that four of the object of the invention provides a kind of.
Herb Gynostemmae Pentaphylli extract provided by the invention is to be raw material with the Herb Gynostemmae Pentaphylli, extracts through the ultrasonic water extraction of ultrasonic water extract-alcohol precipitation or alcohol reflux to make, and main effective ingredient is gypenoside, flavone, polysaccharide.
Herb Gynostemmae Pentaphylli extract provided by the invention makes by the following technical programs:
Technical scheme one
1. Herb Gynostemmae Pentaphylli is pulverized, add 12～20 times of water supersound extraction 3 times, each 1～3h, supersonic frequency 20～80kHz extracts 70～90 ℃ of temperature, and extracting solution merges, add 0.02%～0.2% chitosan clarification, 2～4h, filter, be evaporated to the concentrated solution of relative density 1.05～1.20;
2. add the ethanol mixing in this concentrated solution, adjusting concentration of alcohol is 50%～80%, leaves standstill 24～48h under 4 ℃～10 ℃ conditions, filters with absorbent cotton, and precipitation is filtered with 50%～80% washing with alcohol 1～3 time, and the filtering residue preservation is standby; Merging filtrate, concentrating under reduced pressure reclaim ethanol, relative density is 1.05～1.20 concentrated solution;
3. above-mentioned concentrated solution is crossed the macroporous adsorbent resin chromatographic column, earlier with 10～50 times of water elutions, eluent is preserved standby; With 10～50 times of 50%～80% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts a;
4. above-mentioned filtering residue is added 12～20 times of water supersound extraction 1 time, extraction time 1～3h, supersonic frequency 20～80kHz, extract 70～90 ℃ of temperature, absorbent cotton filters, and filtrate adds 0.02%～0.2% chitosan clarification, 2～4h, filter, filtrate is crossed the macroporous adsorbent resin chromatographic column; With 10～50 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts b that gets of concentrated spray;
5. extract a and extract b are pressed 1%～99%: 1%～99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 6～12KGY
60The Co irradiation sterilization is preserved standby.
Technical scheme two:
1. Herb Gynostemmae Pentaphylli is pulverized, with 1～10 times alcohol reflux 1～3 time, each 1～3h, concentration of alcohol 50%～80%, the medicinal residues reservation is standby;
2. merge ethanol extract, filter with absorbent cotton, precipitation is filtered with 50%～80% ethanol elution 1～3 time, and the filtering residue preservation is standby; Filtrate decompression concentrate to reclaim ethanol, is concentrated into relative density and is 1.05～1.20 concentrated solution;
3. concentrated solution is crossed the macroporous adsorbent resin chromatographic column, with 10～50 times of water elutions, eluent is preserved standby with earlier in elder generation; With 10～50 times of 50%～80% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts A;
4. with 1. medicinal residues and 2. filtering residue merging of step of step, add 12～20 times of water supersound extraction 1～3 time, each 1～3h, supersonic frequency 20～80kHz, extract 70～90 ℃ of temperature, absorbent cotton filters, and filtrate adds 0.02%～0.2% chitosan clarification, 2～4h, filter, filtrate is crossed the macroporous adsorbent resin chromatographic column; With 10～50 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts B that gets of concentrated spray;
5. extract A and extract B are pressed 1%～99%: 1%～99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 6～12KGY
60The Co irradiation sterilization is preserved standby.
Herb Gynostemmae Pentaphylli extract of the present invention mainly contains effective ingredient such as gypenoside, flavone, polysaccharide.
Gynostemma pentaphylla soft capsules preparations of the present invention is that primary raw material is made with the Herb Gynostemmae Pentaphylli extract, contains gypenoside 1%～20%, flavone 1%～30%, any one or a few effective ingredient mixture in the polysaccharide 10%～50%.Gynostemma pentaphylla soft capsules preparations of the present invention is the effective-part of Gynostemma pentaphyllum extract to be added in 50～80 ℃ the solvent dissolve; Add adjuvants such as stabilizing agent again,, cross colloid mill with the abundant mix homogeneously of helical mixer, the degassing, pelleting, typing is dry, and row is coiled drying, selects ball, and polishing makes gynostemma pentaphylla soft capsules preparations of the present invention.
In the gynostemma pentaphylla soft capsules preparations of the present invention, the effective-part of Gynostemma pentaphyllum extract accounts for 10%～50% of soft capsule content, and adjuvants such as stabilizing agent account for 5%～30% of soft capsule content, and the solvent adjuvant accounts for 30%～80%.
Solvent of the present invention can be any one or any several mixture in soybean oil, olive oil, Oleum Arachidis hypogaeae semen, glycerol, Polyethylene Glycol, propylene glycol, the isopropyl alcohol, and consumption is 30%～80% of a soft capsule content; It can be soybean lecithin, Cera Flava, ethyl cellulose that stabilizing agent is decided adjuvant, and the mixture of any one or a few in the aluminum monostearate, consumption are 5%～30% of soft capsule content.
Soft capsule of the present invention is to be that primary raw material is prepared from the effective-part of Gynostemma pentaphyllum extract, is the extract preparation, and dose is little, taking convenience, and the bioavailability height, stable effective ingredients, effect are direct, rapid-action, exact efficacy.Gynostemma pentaphylla soft capsules of the present invention has the spleen invigorating of nourishing heart, benefiting QI for activating blood circulation, and the expectorant blood stasis dispelling, blood fat reducing, antioxidation, human body immunity improving power is improved the function of cardiovascular system.Be applicable to hyperlipidemia, see that had palpitation breathes hard limb fiber crops uncomfortable in chest, dizziness and headache, forgetful tinnitus, heart deficiency of spleen-QI such as unconsciously sweat and tire or distension and fullness in the abdomen, stagnation of phlegm blood stasis person.
The specific embodiment
The following examples help those skilled in the art more fully to understand the present invention, but do not limit the present invention in any way.
Embodiment 1
1. Herb Gynostemmae Pentaphylli 15kg is pulverized, add 12～20 times of water supersound extraction 3 times, each 1～3h, supersonic frequency 20～80kHz extracts 70～90 ℃ of temperature, and extracting solution merges, add 0.02%～0.2% chitosan clarification, 2～4h, filter, be evaporated to the concentrated solution of relative density 1.05～1.20;
2. add the ethanol mixing in this concentrated solution, adjusting concentration of alcohol is 50%～80%, leaves standstill 24～48h under 4 ℃～10 ℃ conditions, filters with absorbent cotton, and precipitation is filtered with 50%～80% washing with alcohol 1～3 time, and the filtering residue preservation is standby; Merging filtrate, concentrating under reduced pressure reclaim ethanol, relative density is 1.05～1.20 concentrated solution;
3. above-mentioned concentrated solution is crossed the macroporous adsorbent resin chromatographic column, earlier with 10～50 times of water elutions, eluent is preserved standby; With 10～50 times of 50%～80% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts a;
4. above-mentioned filtering residue is added 12～20 times of water supersound extraction 1 time, extraction time 1～3h, supersonic frequency 20～80kHz, extract 70～90 ℃ of temperature, absorbent cotton filters, and filtrate adds 0.02%～0.2% chitosan clarification, 2～4h, filter, filtrate is crossed the macroporous adsorbent resin chromatographic column; With 10～50 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts b that gets of concentrated spray;
5. extract a and extract b are pressed 1%～99%: 1%～99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 6～12KGY
60The Co irradiation sterilization is preserved standby.
Embodiment 2
1. Herb Gynostemmae Pentaphylli 15kg is pulverized, add 20 times of water supersound extraction 3 times, each 3h, supersonic frequency 40kHz extracts 85～90 ℃ of temperature, and extracting solution merges, and adds 0.08% chitosan clarification 4h, filters, and is evaporated to the concentrated solution of relative density 1.05～1.20;
2. add the ethanol mixing in this concentrated solution, adjusting concentration of alcohol is 75%, leaves standstill 48h under 4 ℃ of conditions, filters with absorbent cotton, and precipitation is filtered with 75% washing with alcohol 3 times, and the filtering residue preservation is standby; Merging filtrate, concentrating under reduced pressure reclaim ethanol, relative density is 1.05～1.20 concentrated solution;
3. above-mentioned concentrated solution is crossed the macroporous adsorbent resin chromatographic column, earlier with 30 times of water elutions, eluent is preserved standby; With 40 times of 75% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts a;
4. above-mentioned filtering residue is added 15 times of water supersound extraction 1 time, extraction time 3h, supersonic frequency 40kHz extracts 85～90 ℃ of temperature, and absorbent cotton filters, and filtrate adds 0.05% chitosan clarification 4h, filters, and filtrate is crossed the macroporous adsorbent resin chromatographic column; With 20 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts b that gets of concentrated spray;
5. extract a and extract b are pressed 1%～99%: 1%～99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 10KGY
60The Co irradiation sterilization is preserved standby.
Embodiment 3
1. Herb Gynostemmae Pentaphylli 10kg is pulverized, with 1～10 times alcohol reflux 1～3 time, each 1～3h, concentration of alcohol 50%～80%, the medicinal residues reservation is standby;
2. merge ethanol extract, filter with absorbent cotton, precipitation is filtered with 50%～80% ethanol elution 1～3 time, and the filtering residue preservation is standby; Filtrate decompression concentrate to reclaim ethanol, is concentrated into relative density and is 1.05～1.20 concentrated solution;
3. concentrated solution is crossed the macroporous adsorbent resin chromatographic column, with 10～50 times of water elutions, eluent is preserved standby with earlier in elder generation; With 10～50 times of 50%～80% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts A;
4. with 1. medicinal residues and 2. filtering residue merging of step of step, add 12～20 times of water supersound extraction 1～3 time, each 1～3h, supersonic frequency 20～80kHz, extract 70～90 ℃ of temperature, absorbent cotton filters, and filtrate adds 0.02%～0.2% chitosan clarification, 2～4h, filter, filtrate is crossed the macroporous adsorbent resin chromatographic column; With 10～50 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts B that gets of concentrated spray;
5. extract A and extract B are pressed 1%～99%: 1%～99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 6～12KGY
60The Co irradiation sterilization is preserved standby.
Embodiment 4
1. Herb Gynostemmae Pentaphylli 10kg is pulverized, with 10 times alcohol reflux 3 times, each 3h, concentration of alcohol 75%, the medicinal residues reservation is standby;
2. merge ethanol extract, filter with absorbent cotton, precipitation is filtered with 75% ethanol elution 3 times, and the filtering residue preservation is standby; Filtrate decompression concentrate to reclaim ethanol, is concentrated into relative density and is 1.05～1.20 concentrated solution;
3. concentrated solution is crossed the macroporous adsorbent resin chromatographic column, with 30 times of water elutions, eluent is preserved standby with earlier in elder generation; With 40 times of 75% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts A;
4. with 1. medicinal residues and 2. filtering residue merging of step of step, add 20 times of water supersound extraction 3 times, each 3h, supersonic frequency 40kHz extracts 85～90 ℃ of temperature, and absorbent cotton filters, and filtrate adds 0.1% chitosan clarification 4h, filtration, filtrate is crossed the macroporous adsorbent resin chromatographic column; With 20 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts B that gets of concentrated spray;
5. extract A and extract B are pressed 1%～99%: 1%～99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 6～12KGY
60The Co irradiation sterilization is preserved standby.
Embodiment 5
Get the Herb Gynostemmae Pentaphylli extract 220g that obtains among the embodiment 2, add among 65 ℃ the soybean oil 690g fully stirring and dissolving, add Cera Flava 50g again, soybean lecithin 30g, ethyl cellulose 10g with the abundant mix homogeneously of helical mixer, cross colloid mill, the degassing, pelleting, typing is dry, and row is coiled drying, select ball, polishing makes 2000 of gynostemma pentaphylla soft capsules preparations of the present invention, the heavy 0.5g of every capsules.
Embodiment 6
Get the Herb Gynostemmae Pentaphylli extract 180g that obtains among the embodiment 4, add among 65 ℃ the soybean oil 750g fully stirring and dissolving, add Cera Flava 40g again, soybean lecithin 20g, aluminum monostearate 10g with the abundant mix homogeneously of helical mixer, cross colloid mill, the degassing, pelleting, typing is dry, and row is coiled drying, select ball, polishing makes 2000 of gynostemma pentaphylla soft capsules preparations of the present invention, the heavy 0.5g of every capsules.
Claims (2)
1. a effective-part of Gynostemma pentaphyllum extract contains gypenoside 1%～20%, flavone 1%～30%, and polysaccharide 10%～50% is characterized in that, the effective-part of Gynostemma pentaphyllum preparation method of extract is:
1. Herb Gynostemmae Pentaphylli is pulverized, add 12～20 times of water supersound extraction 3 times, each 1～3h, supersonic frequency 20～80kHz extracts 70～90 ℃ of temperature, and extracting solution merges, add 0.02%～0.2% chitosan clarification, 2～4h, filter, be evaporated to the concentrated solution of relative density 1.05～1.20;
2. add the ethanol mixing in this concentrated solution, adjusting concentration of alcohol is 50%～80%, leaves standstill 24～48h under 4 ℃～10 ℃ conditions, filters with absorbent cotton, and precipitation is filtered with 50%～80% washing with alcohol 1～3 time, and the filtering residue preservation is standby; Merging filtrate, concentrating under reduced pressure reclaim ethanol, relative density is 1.05～1.20 concentrated solution;
3. above-mentioned concentrated solution is crossed the macroporous adsorbent resin chromatographic column, earlier with 10～50 times of water elutions, eluent is preserved standby; With 10～50 times of 50%～80% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts a;
4. above-mentioned filtering residue is added 12～20 times of water supersound extraction 1 time, extraction time 1～3h, supersonic frequency 20～80kHz, extract 70～90 ℃ of temperature, absorbent cotton filters, and filtrate adds 0.02%～0.2% chitosan clarification, 2～4h, filter, filtrate is crossed the macroporous adsorbent resin chromatographic column; With 10～50 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts b that gets of concentrated spray;
5. extract a and extract b are pressed 1%～99%: 1%～99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 6～12KGY
60The Co irradiation sterilization is preserved standby.
2. a kind of the effective-part of Gynostemma pentaphyllum extract according to claim 1 is characterized in that the effective-part of Gynostemma pentaphyllum preparation method of extract is:
1. Herb Gynostemmae Pentaphylli is pulverized, add 20 times of water supersound extraction 3 times, each 3h, supersonic frequency 40kHz extracts 85～90 ℃ of temperature, and extracting solution merges, and adds 0.08% chitosan clarification 4h, filters, and is evaporated to the concentrated solution of relative density 1.05～1.20;
2. add the ethanol mixing in this concentrated solution, adjusting concentration of alcohol is 75%, leaves standstill 48h under 4 ℃ of conditions, filters with absorbent cotton, and precipitation is filtered with 75% washing with alcohol 3 times, and the filtering residue preservation is standby; Merging filtrate, concentrating under reduced pressure reclaim ethanol, relative density is 1.05～1.20 concentrated solution;
3. above-mentioned concentrated solution is crossed the macroporous adsorbent resin chromatographic column, earlier with 30 times of water elutions, eluent is preserved standby; With 40 times of 75% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts a;
4. above-mentioned filtering residue is added 15 times of water supersound extraction 1 time, extraction time 3h, supersonic frequency 40kHz extracts 85～90 ℃ of temperature, and absorbent cotton filters, and filtrate adds 0.05% chitosan clarification 4h, filters, and filtrate is crossed the macroporous adsorbent resin chromatographic column; With 20 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts b that gets of concentrated spray;
5. extract a and extract b are pressed 1%～99%: 1%～99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 10KGY
60The Co irradiation sterilization is preserved standby.
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| CN201010151835.9A Division CN101829180B (en) | 2008-03-31 | 2008-03-31 | Gynostemma pentaphyllum soft capsule preparation and preparation method thereof |
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| CN101717452B (en) * | 2009-09-14 | 2011-08-03 | 西北工业大学 | Method for preparing neutral polysaccharide from gynostemma pentaphylla |
| CN102504038B (en) * | 2011-10-28 | 2013-08-21 | 浙江中烟工业有限责任公司 | Plant polysaccharide and preparation method thereof as well as application of plant polysaccharide in cigarette |
| CN109054987A (en) * | 2018-08-30 | 2018-12-21 | 广西那坡县翠株园林业科技有限公司 | A kind of production method of tea oil |
| CN109123673A (en) * | 2018-09-03 | 2019-01-04 | 西安禾其果商贸股份有限公司 | Selenium-rich gynostemma pentaphylla extract and the preparation method and application thereof |
| CN113331316A (en) * | 2021-06-18 | 2021-09-03 | 天津纳尔生物科技有限公司 | Eucalyptus oil-containing laying hen feed and production method thereof |
| CN114424823B (en) * | 2022-01-20 | 2023-07-04 | 江苏海王健康生物科技有限公司 | A soft capsule containing natural extract for reducing blood lipid and its preparation method |
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| 张育松等.绞股蓝干品、绞股蓝鲜品中皂苷、黄酮和多糖的提取条件及其饮料的加工工艺.福建农林大学学报36 5.2007,36(5),532-536. * |
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