CN101829181A - Extract of the effective-part of Gynostemma pentaphyllum and preparation method thereof - Google Patents
Extract of the effective-part of Gynostemma pentaphyllum and preparation method thereof Download PDFInfo
- Publication number
- CN101829181A CN101829181A CN201010151843.3A CN201010151843A CN101829181A CN 101829181 A CN101829181 A CN 101829181A CN 201010151843 A CN201010151843 A CN 201010151843A CN 101829181 A CN101829181 A CN 101829181A
- Authority
- CN
- China
- Prior art keywords
- extract
- gynostemma pentaphyllum
- effective
- ethanol
- standby
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000284 extract Substances 0.000 title claims abstract description 75
- 235000002956 Gynostemma pentaphyllum Nutrition 0.000 title claims abstract description 37
- 240000006509 Gynostemma pentaphyllum Species 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 83
- 238000000605 extraction Methods 0.000 claims abstract description 18
- 238000001556 precipitation Methods 0.000 claims abstract description 10
- 238000010992 reflux Methods 0.000 claims abstract description 7
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims abstract description 6
- 229930003944 flavone Natural products 0.000 claims abstract description 6
- 150000002212 flavone derivatives Chemical class 0.000 claims abstract description 6
- 235000011949 flavones Nutrition 0.000 claims abstract description 6
- 150000004676 glycans Chemical class 0.000 claims abstract description 6
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 6
- 239000005017 polysaccharide Substances 0.000 claims abstract description 6
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000010828 elution Methods 0.000 claims description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 239000003480 eluent Substances 0.000 claims description 24
- 239000000706 filtrate Substances 0.000 claims description 24
- 238000001914 filtration Methods 0.000 claims description 18
- 229920000742 Cotton Polymers 0.000 claims description 16
- 239000003463 adsorbent Substances 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 16
- 239000011347 resin Substances 0.000 claims description 16
- 229920005989 resin Polymers 0.000 claims description 16
- 229920001661 Chitosan Polymers 0.000 claims description 11
- 238000005352 clarification Methods 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 8
- 238000004321 preservation Methods 0.000 claims description 8
- 239000007921 spray Substances 0.000 claims description 8
- 238000001694 spray drying Methods 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 239000012141 concentrate Substances 0.000 claims description 5
- 230000006837 decompression Effects 0.000 claims description 5
- 239000000469 ethanolic extract Substances 0.000 claims description 5
- 229930187479 gypenoside Natural products 0.000 claims description 5
- ZRBFCAALKKNCJG-UHFFFAOYSA-N gypenoside-XVII Natural products C1CC(C2(CCC3C(C)(C)C(OC4C(C(O)C(O)C(CO)O4)O)CCC3(C)C2CC2O)C)(C)C2C1C(C)(CCC=C(C)C)OC(C(C(O)C1O)O)OC1COC1OC(CO)C(O)C(O)C1O ZRBFCAALKKNCJG-UHFFFAOYSA-N 0.000 claims description 5
- 239000007901 soft capsule Substances 0.000 abstract description 20
- 239000008280 blood Substances 0.000 abstract description 7
- 210000004369 blood Anatomy 0.000 abstract description 7
- 239000004615 ingredient Substances 0.000 abstract description 6
- 238000001035 drying Methods 0.000 abstract description 5
- 239000000084 colloidal system Substances 0.000 abstract description 4
- 238000007872 degassing Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 238000005498 polishing Methods 0.000 abstract description 4
- 239000002904 solvent Substances 0.000 abstract description 4
- 239000003381 stabilizer Substances 0.000 abstract description 4
- 206010062717 Increased upper airway secretion Diseases 0.000 abstract description 3
- 210000000748 cardiovascular system Anatomy 0.000 abstract description 3
- 208000026435 phlegm Diseases 0.000 abstract description 3
- 230000003213 activating effect Effects 0.000 abstract description 2
- 230000017531 blood circulation Effects 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract description 2
- 210000000952 spleen Anatomy 0.000 abstract description 2
- 238000007599 discharging Methods 0.000 abstract 1
- 150000002632 lipids Chemical class 0.000 abstract 1
- 238000009740 moulding (composite fabrication) Methods 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 239000008188 pellet Substances 0.000 abstract 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 abstract 1
- 229930182490 saponin Natural products 0.000 abstract 1
- 150000007949 saponins Chemical class 0.000 abstract 1
- 238000005728 strengthening Methods 0.000 abstract 1
- 230000001502 supplementing effect Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 description 6
- 239000002994 raw material Substances 0.000 description 5
- 239000002671 adjuvant Substances 0.000 description 4
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241001065361 Gynostemma Species 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 239000007766 cera flava Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 229940083466 soybean lecithin Drugs 0.000 description 3
- 239000003549 soybean oil Substances 0.000 description 3
- 235000012424 soybean oil Nutrition 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 239000001856 Ethyl cellulose Substances 0.000 description 2
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- UGMCXQCYOVCMTB-UHFFFAOYSA-K dihydroxy(stearato)aluminium Chemical compound CCCCCCCCCCCCCCCCCC(=O)O[Al](O)O UGMCXQCYOVCMTB-UHFFFAOYSA-K 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229920001249 ethyl cellulose Polymers 0.000 description 2
- 235000019325 ethyl cellulose Nutrition 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 241000219104 Cucurbitaceae Species 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010033557 Palpitations Diseases 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 208000009205 Tinnitus Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000003172 expectorant agent Substances 0.000 description 1
- 230000003419 expectorant effect Effects 0.000 description 1
- 201000005577 familial hyperlipidemia Diseases 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 231100000886 tinnitus Toxicity 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an extract of the effective-part of Gynostemma pentaphyllum and a preparation method thereof. The extract of the effective-part of gynostemma pentaphyllum contains gynostemma pentaphyllum saponin, flavone and polysaccharide and is prepared from gynostemma pentaphyllum through ultrasonic aqueous extraction and alcohol precipitation or through the alcohol reflux ultrasonic aqueous extraction method. The preparation method comprises the following steps: adding the extract of the effective-part of gynostemma pentaphyllum in 50-80 DEG C of solvent to dissolve; then adding stabilizer and other auxiliary ingredients, using a spiral mixer to mix completely and evenly, passing through a colloid mill, degassing, pelleting, forming, drying, discharging in trays, drying, selecting pellets and polishing to prepare gynostemma pentaphyllum soft capsule preparation. The soft capsule has small dosage, convenient taking way, fast onset of efficacy and obvious effect and has the functions of nourishing heart, strengthening the spleen, supplementing qi, activating blood circulation, clearing phlegm, removing blood stasis, reducing blood lipid, resisting oxidation, increasing the immunity of the organism and improving the cardiovascular system.
Description
The application is " 2008.3.31 " to the applying date; Application number is " 200810103117.7 "; Denomination of invention is divided an application for the application for a patent for invention of " gynostemma pentaphylla soft capsules preparations and preparation method thereof ".
Technical field
The present invention relates to a kind of Chinese medicinal soft capsule preparation and easy to prepare, relate in particular to a kind of gynostemma pentaphylla soft capsules preparations and preparation method thereof, belong to medical technical field.
Background technology
Herb Gynostemmae Pentaphylli [Gynostemma pentaphyllum (thunb) mak] is a cucurbitaceae genus gynostemma.Herb Gynostemmae Pentaphylli contains multiple gypenoside to the human body beneficial, flavone, vitamin, aminoacid and polysaccharide.Studies show that the Herb Gynostemmae Pentaphylli active ingredient has effects such as blood fat reducing, blood sugar lowering, antitumor, defying age, resisting fatigue, the liver protecting and enhancing body immune function.Diseases such as Cardiovascular system disorders such as hyperlipemia, hypertension, coronary heart disease, diabetes, tumor had good prevention effect.Long-term edible having no side effect is described as China's " southern Radix Ginseng ", " anticancer rising star ".Motherland's medical science is thought, Herb Gynostemmae Pentaphylli bitter in the mouth, cold in nature, nontoxic.Eliminating inflammation and expelling toxin, the eliminating phlegm and stopping cough of being used among the people, the producing region masses adopt its stem and leaf in summer and fry in shallow oil water and make refreshment drink more.
Effective ingredient for the most effective extraction and raising Herb Gynostemmae Pentaphylli guarantees making full use of of Herb Gynostemmae Pentaphylli, and the increase drug effect reduces cost and increases economic efficiency, and the present invention overcomes the prior art deficiency, and a kind of gynostemma pentaphylla soft capsules preparations and preparation method thereof is provided.
Summary of the invention
One of purpose of the present invention provides a kind of the effective-part of Gynostemma pentaphyllum extract; Two of the object of the invention provides a kind of the effective-part of Gynostemma pentaphyllum preparation method of extract; It is the soft capsule preparation of primary raw material with the Herb Gynostemmae Pentaphylli effective part extract that three of the object of the invention provides a kind of; It is the preparation method of the soft capsule preparation of primary raw material with the Herb Gynostemmae Pentaphylli effective part extract that four of the object of the invention provides a kind of.
Herb Gynostemmae Pentaphylli extract provided by the invention is to be raw material with the Herb Gynostemmae Pentaphylli, extracts through the ultrasonic water extraction of ultrasonic water extract-alcohol precipitation or alcohol reflux to make, and main effective ingredient is gypenoside, flavone, polysaccharide.
Herb Gynostemmae Pentaphylli extract provided by the invention makes by the following technical programs:
Technical scheme one
1. Herb Gynostemmae Pentaphylli is pulverized, add 12~20 times of water supersound extraction 3 times, each 1~3h, supersonic frequency 20~80kHz extracts 70~90 ℃ of temperature, and extracting solution merges, add 0.02%~0.2% chitosan clarification, 2~4h, filter, be evaporated to the concentrated solution of relative density 1.05~1.20;
2. add the ethanol mixing in this concentrated solution, adjusting concentration of alcohol is 50%~80%, leaves standstill 24~48h under 4 ℃~10 ℃ conditions, filters with absorbent cotton, and precipitation is filtered with 50%~80% washing with alcohol 1~3 time, and the filtering residue preservation is standby; Merging filtrate, concentrating under reduced pressure reclaim ethanol, relative density is 1.05~1.20 concentrated solution;
3. above-mentioned concentrated solution is crossed the macroporous adsorbent resin chromatographic column, earlier with 10~50 times of water elutions, eluent is preserved standby; With 10~50 times of 50%~80% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts a;
4. above-mentioned filtering residue is added 12~20 times of water supersound extraction 1 time, extraction time 1~3h, supersonic frequency 20~80kHz, extract 70~90 ℃ of temperature, absorbent cotton filters, and filtrate adds 0.02%~0.2% chitosan clarification, 2~4h, filter, filtrate is crossed the macroporous adsorbent resin chromatographic column; With 10~50 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts b that gets of concentrated spray;
5. extract a and extract b are pressed 1%~99%: 1%~99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 6~12KGY
60The Co irradiation sterilization is preserved standby.
Technical scheme two:
1. Herb Gynostemmae Pentaphylli is pulverized, with 1~10 times alcohol reflux 1~3 time, each 1~3h, concentration of alcohol 50%~80%, the medicinal residues reservation is standby;
2. merge ethanol extract, filter with absorbent cotton, precipitation is filtered with 50%~80% ethanol elution 1~3 time, and the filtering residue preservation is standby; Filtrate decompression concentrate to reclaim ethanol, is concentrated into relative density and is 1.05~1.20 concentrated solution;
3. concentrated solution is crossed the macroporous adsorbent resin chromatographic column, with 10~50 times of water elutions, eluent is preserved standby with earlier in elder generation; With 10~50 times of 50%~80% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts A;
4. with 1. medicinal residues and 2. filtering residue merging of step of step, add 12~20 times of water supersound extraction 1~3 time, each 1~3h, supersonic frequency 20~80kHz, extract 70~90 ℃ of temperature, absorbent cotton filters, and filtrate adds 0.02%~0.2% chitosan clarification, 2~4h, filter, filtrate is crossed the macroporous adsorbent resin chromatographic column; With 10~50 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts B that gets of concentrated spray;
5. extract A and extract B are pressed 1%~99%: 1%~99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 6~12KGY
60The Co irradiation sterilization is preserved standby.
Herb Gynostemmae Pentaphylli extract of the present invention mainly contains effective ingredient such as gypenoside, flavone, polysaccharide.
Gynostemma pentaphylla soft capsules preparations of the present invention is that primary raw material is made with the Herb Gynostemmae Pentaphylli extract, contains gypenoside 1%~20%, flavone 1%~30%, any one or a few effective ingredient mixture in the polysaccharide 10%~50%.Gynostemma pentaphylla soft capsules preparations of the present invention is the effective-part of Gynostemma pentaphyllum extract to be added in 50~80 ℃ the solvent dissolve; Add adjuvants such as stabilizing agent again,, cross colloid mill with the abundant mix homogeneously of helical mixer, the degassing, pelleting, typing is dry, and row is coiled drying, selects ball, and polishing makes gynostemma pentaphylla soft capsules preparations of the present invention.
In the gynostemma pentaphylla soft capsules preparations of the present invention, the effective-part of Gynostemma pentaphyllum extract accounts for 10%~50% of soft capsule content, and adjuvants such as stabilizing agent account for 5%~30% of soft capsule content, and the solvent adjuvant accounts for 30%~80%.
Solvent of the present invention can be any one or any several mixture in soybean oil, olive oil, Oleum Arachidis hypogaeae semen, glycerol, Polyethylene Glycol, propylene glycol, the isopropyl alcohol, and consumption is 30%~80% of a soft capsule content; It can be soybean lecithin, Cera Flava, ethyl cellulose that stabilizing agent is decided adjuvant, and the mixture of any one or a few in the aluminum monostearate, consumption are 5%~30% of soft capsule content.
Soft capsule of the present invention is to be that primary raw material is prepared from the effective-part of Gynostemma pentaphyllum extract, is the extract preparation, and dose is little, taking convenience, and the bioavailability height, stable effective ingredients, effect are direct, rapid-action, exact efficacy.Gynostemma pentaphylla soft capsules of the present invention has the spleen invigorating of nourishing heart, benefiting QI for activating blood circulation, and the expectorant blood stasis dispelling, blood fat reducing, antioxidation, human body immunity improving power is improved the function of cardiovascular system.Be applicable to hyperlipidemia, see that had palpitation breathes hard limb fiber crops uncomfortable in chest, dizziness and headache, forgetful tinnitus, heart deficiency of spleen-QI such as unconsciously sweat and tire or distension and fullness in the abdomen, stagnation of phlegm blood stasis person.
The specific embodiment
The following examples help those skilled in the art more fully to understand the present invention, but do not limit the present invention in any way.
Embodiment 1
1. Herb Gynostemmae Pentaphylli 15kg is pulverized, add 12~20 times of water supersound extraction 3 times, each 1~3h, supersonic frequency 20~80kHz extracts 70~90 ℃ of temperature, and extracting solution merges, add 0.02%~0.2% chitosan clarification, 2~4h, filter, be evaporated to the concentrated solution of relative density 1.05~1.20;
2. add the ethanol mixing in this concentrated solution, adjusting concentration of alcohol is 50%~80%, leaves standstill 24~48h under 4 ℃~10 ℃ conditions, filters with absorbent cotton, and precipitation is filtered with 50%~80% washing with alcohol 1~3 time, and the filtering residue preservation is standby; Merging filtrate, concentrating under reduced pressure reclaim ethanol, relative density is 1.05~1.20 concentrated solution;
3. above-mentioned concentrated solution is crossed the macroporous adsorbent resin chromatographic column, earlier with 10~50 times of water elutions, eluent is preserved standby; With 10~50 times of 50%~80% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts a;
4. above-mentioned filtering residue is added 12~20 times of water supersound extraction 1 time, extraction time 1~3h, supersonic frequency 20~80kHz, extract 70~90 ℃ of temperature, absorbent cotton filters, and filtrate adds 0.02%~0.2% chitosan clarification, 2~4h, filter, filtrate is crossed the macroporous adsorbent resin chromatographic column; With 10~50 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts b that gets of concentrated spray;
5. extract a and extract b are pressed 1%~99%: 1%~99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 6~12KGY
60The Co irradiation sterilization is preserved standby.
Embodiment 2
1. Herb Gynostemmae Pentaphylli 15kg is pulverized, add 20 times of water supersound extraction 3 times, each 3h, supersonic frequency 40kHz extracts 85~90 ℃ of temperature, and extracting solution merges, and adds 0.08% chitosan clarification 4h, filters, and is evaporated to the concentrated solution of relative density 1.05~1.20;
2. add the ethanol mixing in this concentrated solution, adjusting concentration of alcohol is 75%, leaves standstill 48h under 4 ℃ of conditions, filters with absorbent cotton, and precipitation is filtered with 75% washing with alcohol 3 times, and the filtering residue preservation is standby; Merging filtrate, concentrating under reduced pressure reclaim ethanol, relative density is 1.05~1.20 concentrated solution;
3. above-mentioned concentrated solution is crossed the macroporous adsorbent resin chromatographic column, earlier with 30 times of water elutions, eluent is preserved standby; With 40 times of 75% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts a;
4. above-mentioned filtering residue is added 15 times of water supersound extraction 1 time, extraction time 3h, supersonic frequency 40kHz extracts 85~90 ℃ of temperature, and absorbent cotton filters, and filtrate adds 0.05% chitosan clarification 4h, filters, and filtrate is crossed the macroporous adsorbent resin chromatographic column; With 20 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts b that gets of concentrated spray;
5. extract a and extract b are pressed 1%~99%: 1%~99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 10KGY
60The Co irradiation sterilization is preserved standby.
Embodiment 3
1. Herb Gynostemmae Pentaphylli 10kg is pulverized, with 1~10 times alcohol reflux 1~3 time, each 1~3h, concentration of alcohol 50%~80%, the medicinal residues reservation is standby;
2. merge ethanol extract, filter with absorbent cotton, precipitation is filtered with 50%~80% ethanol elution 1~3 time, and the filtering residue preservation is standby; Filtrate decompression concentrate to reclaim ethanol, is concentrated into relative density and is 1.05~1.20 concentrated solution;
3. concentrated solution is crossed the macroporous adsorbent resin chromatographic column, with 10~50 times of water elutions, eluent is preserved standby with earlier in elder generation; With 10~50 times of 50%~80% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts A;
4. with 1. medicinal residues and 2. filtering residue merging of step of step, add 12~20 times of water supersound extraction 1~3 time, each 1~3h, supersonic frequency 20~80kHz, extract 70~90 ℃ of temperature, absorbent cotton filters, and filtrate adds 0.02%~0.2% chitosan clarification, 2~4h, filter, filtrate is crossed the macroporous adsorbent resin chromatographic column; With 10~50 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts B that gets of concentrated spray;
5. extract A and extract B are pressed 1%~99%: 1%~99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 6~12KGY
60The Co irradiation sterilization is preserved standby.
Embodiment 4
1. Herb Gynostemmae Pentaphylli 10kg is pulverized, with 10 times alcohol reflux 3 times, each 3h, concentration of alcohol 75%, the medicinal residues reservation is standby;
2. merge ethanol extract, filter with absorbent cotton, precipitation is filtered with 75% ethanol elution 3 times, and the filtering residue preservation is standby; Filtrate decompression concentrate to reclaim ethanol, is concentrated into relative density and is 1.05~1.20 concentrated solution;
3. concentrated solution is crossed the macroporous adsorbent resin chromatographic column, with 30 times of water elutions, eluent is preserved standby with earlier in elder generation; With 40 times of 75% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts A;
4. with 1. medicinal residues and 2. filtering residue merging of step of step, add 20 times of water supersound extraction 3 times, each 3h, supersonic frequency 40kHz extracts 85~90 ℃ of temperature, and absorbent cotton filters, and filtrate adds 0.1% chitosan clarification 4h, filtration, filtrate is crossed the macroporous adsorbent resin chromatographic column; With 20 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts B that gets of concentrated spray;
5. extract A and extract B are pressed 1%~99%: 1%~99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 6~12KGY
60The Co irradiation sterilization is preserved standby.
Embodiment 5
Get the Herb Gynostemmae Pentaphylli extract 220g that obtains among the embodiment 2, add among 65 ℃ the soybean oil 690g fully stirring and dissolving, add Cera Flava 50g again, soybean lecithin 30g, ethyl cellulose 10g with the abundant mix homogeneously of helical mixer, cross colloid mill, the degassing, pelleting, typing is dry, and row is coiled drying, select ball, polishing makes 2000 of gynostemma pentaphylla soft capsules preparations of the present invention, the heavy 0.5g of every capsules.
Embodiment 6
Get the Herb Gynostemmae Pentaphylli extract 180g that obtains among the embodiment 4, add among 65 ℃ the soybean oil 750g fully stirring and dissolving, add Cera Flava 40g again, soybean lecithin 20g, aluminum monostearate 10g with the abundant mix homogeneously of helical mixer, cross colloid mill, the degassing, pelleting, typing is dry, and row is coiled drying, select ball, polishing makes 2000 of gynostemma pentaphylla soft capsules preparations of the present invention, the heavy 0.5g of every capsules.
Claims (2)
1. a effective-part of Gynostemma pentaphyllum extract contains gypenoside 1%~20%, flavone 1%~30%, and polysaccharide 10%~50% is characterized in that, the effective-part of Gynostemma pentaphyllum preparation method of extract is:
1. Herb Gynostemmae Pentaphylli is pulverized, with 1~10 times alcohol reflux 1~3 time, each 1~3h, concentration of alcohol 50%~80%, the medicinal residues reservation is standby;
2. merge ethanol extract, filter with absorbent cotton, precipitation is filtered with 50%~80% ethanol elution 1~3 time, and the filtering residue preservation is standby; Filtrate decompression concentrate to reclaim ethanol, is concentrated into relative density and is 1.05~1.20 concentrated solution;
3. concentrated solution is crossed the macroporous adsorbent resin chromatographic column, with 10~50 times of water elutions, eluent is preserved standby with earlier in elder generation; With 10~50 times of 50%~80% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts A;
4. with 1. medicinal residues and 2. filtering residue merging of step of step, add 12~20 times of water supersound extraction 1~3 time, each 1~3h, supersonic frequency 20~80kHz, extract 70~90 ℃ of temperature, absorbent cotton filters, and filtrate adds 0.02%~0.2% chitosan clarification, 2~4h, filter, filtrate is crossed the macroporous adsorbent resin chromatographic column; With 10~50 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts B that gets of concentrated spray;
5. extract A and extract B are pressed 1%~99%: 1%~99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 6~12KGY
60The Co irradiation sterilization is preserved standby.
2. a kind of the effective-part of Gynostemma pentaphyllum extract according to claim 1 is characterized in that the effective-part of Gynostemma pentaphyllum preparation method of extract is:
1. Herb Gynostemmae Pentaphylli is pulverized, with 10 times alcohol reflux 3 times, each 3h, concentration of alcohol 75%, the medicinal residues reservation is standby;
2. merge ethanol extract, filter with absorbent cotton, precipitation is filtered with 75% ethanol elution 3 times, and the filtering residue preservation is standby; Filtrate decompression concentrate to reclaim ethanol, is concentrated into relative density and is 1.05~1.20 concentrated solution;
3. concentrated solution is crossed the macroporous adsorbent resin chromatographic column, with 30 times of water elutions, eluent is preserved standby with earlier in elder generation; With 40 times of 75% ethanol elution, the eluent concentrating under reduced pressure reclaims ethanol again, and spray drying gets powder extracts A;
4. with 1. medicinal residues and 2. filtering residue merging of step of step, add 20 times of water supersound extraction 3 times, each 3h, supersonic frequency 40kHz extracts 85~90 ℃ of temperature, and absorbent cotton filters, and filtrate adds 0.1% chitosan clarification 4h, filtration, filtrate is crossed the macroporous adsorbent resin chromatographic column; With 20 times of water elutions; Eluent and step 3. gained water elution liquid merge, the dry powder extracts B that gets of concentrated spray;
5. extract A and extract B are pressed 1%~99%: 1%~99% mixed, promptly get the effective-part of Gynostemma pentaphyllum extract, through 6~12KGY
60The Co irradiation sterilization is preserved standby.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201010151843.3A CN101829181A (en) | 2008-03-31 | 2008-03-31 | Extract of the effective-part of Gynostemma pentaphyllum and preparation method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201010151843.3A CN101829181A (en) | 2008-03-31 | 2008-03-31 | Extract of the effective-part of Gynostemma pentaphyllum and preparation method thereof |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200810103117.7A Division CN101249131B (en) | 2008-03-31 | 2008-03-31 | Gynostemma pentaphylla effective ingredient extract |
Publications (1)
Publication Number | Publication Date |
---|---|
CN101829181A true CN101829181A (en) | 2010-09-15 |
Family
ID=42713502
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201010151843.3A Pending CN101829181A (en) | 2008-03-31 | 2008-03-31 | Extract of the effective-part of Gynostemma pentaphyllum and preparation method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN101829181A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102652764A (en) * | 2012-04-26 | 2012-09-05 | 安徽省芬格欣普蓝生物药业有限公司 | Gynostemma extract extraction method implemented through carrying out wall breaking firstly |
CN104982777A (en) * | 2015-06-17 | 2015-10-21 | 蚌埠市天星树脂有限责任公司 | Resin for original material extraction of spleen invigorating and qi benefiting health care medicine and preparation method thereof |
CN105532976A (en) * | 2015-12-22 | 2016-05-04 | 桂林双象生物科技有限公司 | Strigose hydrangea leaf and herba gynostemmatis pentaphylli compound original tea preparation method |
CN106963803A (en) * | 2017-03-31 | 2017-07-21 | 浙江工业大学 | Application of the gynostemma pentaphyllum total flavones in the medicine for preparing preventing and treating myocardial hypertrophy |
-
2008
- 2008-03-31 CN CN201010151843.3A patent/CN101829181A/en active Pending
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102652764A (en) * | 2012-04-26 | 2012-09-05 | 安徽省芬格欣普蓝生物药业有限公司 | Gynostemma extract extraction method implemented through carrying out wall breaking firstly |
CN104982777A (en) * | 2015-06-17 | 2015-10-21 | 蚌埠市天星树脂有限责任公司 | Resin for original material extraction of spleen invigorating and qi benefiting health care medicine and preparation method thereof |
CN105532976A (en) * | 2015-12-22 | 2016-05-04 | 桂林双象生物科技有限公司 | Strigose hydrangea leaf and herba gynostemmatis pentaphylli compound original tea preparation method |
CN106963803A (en) * | 2017-03-31 | 2017-07-21 | 浙江工业大学 | Application of the gynostemma pentaphyllum total flavones in the medicine for preparing preventing and treating myocardial hypertrophy |
CN106963803B (en) * | 2017-03-31 | 2020-10-09 | 浙江工业大学 | Application of gynostemma pentaphylla total flavone in preparing medicine for preventing and treating cardiac hypertrophy |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101703623A (en) | Capsule with effect of relieving physical fatigue | |
WO2016173509A1 (en) | Compositions comprising cyclocarya paliurus extract and preparation method and uses thereof | |
CN101703221B (en) | Food supplement for incretion regulation, blood enrichment, skin maintenance, hairdressing and speckle removal and preparation method thereof | |
CN101249131B (en) | Gynostemma pentaphylla effective ingredient extract | |
CN107375807A (en) | Yin-nourishing and health composition and preparation method | |
CN105265672A (en) | Diaphragma juglandis kidney supplementing and sperm producing health preserving tea and production method thereof | |
KR20190045057A (en) | Dietary herbal composition for improving obesity and method for producing the same | |
CN101829181A (en) | Extract of the effective-part of Gynostemma pentaphyllum and preparation method thereof | |
CN102266487B (en) | Kudzuvine root-containing composition for treating hyperlipemia and preparation method and application thereof | |
CN103798473A (en) | Longevity healthcare tea | |
CN104666643B (en) | A kind of compound snow chrysanthemum product and its preparation method and application | |
CN103479691A (en) | Lipid-decreasing liver protection traditional Chinese medicine composition | |
CN105266149A (en) | Herba dendrodii officinalis compound health-care capsule and preparation method thereof | |
CN104115960A (en) | Fresh reed rhizome herbal tea beverage | |
CN101829180B (en) | Gynostemma pentaphyllum soft capsule preparation and preparation method thereof | |
CN108684992A (en) | A kind of alcohol-decomposing beverage of plant formula and preparation method thereof of energy Quick sobering | |
CN110538311A (en) | Pure traditional Chinese medicine composition for controlling hypertension and preparation method thereof | |
CN103656226A (en) | Compound medicine for treating sicca syndrome | |
CN102846720B (en) | Composite for lowering blood fat and improving anoxia endurance | |
CN105582334A (en) | Whole-plant healthcare product | |
CN111011852A (en) | Preparation method of ginseng instant food with liver protection function | |
CN100364584C (en) | Method for preparing compound micron pini-konnjaku juice fat-reducing sugar-reducing oral liquor | |
CN110090249A (en) | Prevent and treat the Chinese medicine composition and the preparation method and application thereof of hypertension | |
CN104474337A (en) | Preparation method of Chinese patent health granule containing green tea extract | |
CN107581443A (en) | A kind of granular solids beverage and preparation method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
AD01 | Patent right deemed abandoned |
Effective date of abandoning: 20100915 |
|
C20 | Patent right or utility model deemed to be abandoned or is abandoned |