CN101224237B - Novel uses of compound seabuckthorn seed oil in preparing medicine - Google Patents
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Abstract
The invention discloses a novel application of compound seabuckthorn oil in pharmacy, that is, the compound seabuckthorn oil in preparing drugs for repairing trauma surface after physical therapy of cervical erosion and light to medium degree atypical hyperplasia of cervical intraepithelial neoplasia. Proved by pharmacodynamics test and study, the compound seabuckthorn oil suppository has a certain treatment effect on animal vagina and cervicitis model caused by chemical burn and biological factors infection and on light to medium degree atypical hyperplasia of cervical intraepithelial neoplasia and can present preclinical effectiveness on the therapy of cervical erosion and on light to medium degree atypical hyperplasia of cervical intraepithelial neoplasia.
Description
Technical field: the present invention relates to the new purposes of compound seabuckthorn fruit oil in pharmacy, particularly the purposes of the medicine of light, the moderate atypical hyperplasia of the reparation of compound seabuckthorn fruit oil wound face after preparation is used for the cervical erosion naturopathy and cervical intraepithelial neoplasia (CIN) belongs to technical field of Chinese medicines.
Background technology: cervical erosion is the modal a kind of pathological change of chronic cervicitis, is owing to inflammatory stimulus, the covering epithelium cell detachment on cervix uteri surface, and the columnar epithelial cell of below is outwards outstanding, has replaced the covering epithelium that comes off; Because of columnar epithelial cell blood capillary appears, make the inflammation district be cerise, so be called cervical erosion.Rotten to the corn face is covered by complete cervical canal simple columnar epithelium, and because of columnar epithelium is poor, matter appeared and takes on a red color between it was following, was not true erosion, and the true erosion pathology refer to exuviation ulcer.
Wound face behind the cervical erosion naturopathy is meant the wound that stays behind cervical erosion underwent operative or the naturopathy, takes the speed that can improve the wound face recovery and heal behind certain medicine, the domestic medicine that does not also have this effect at present.
Cervical intraepithelial neoplasia (CIN) is light, moderate atypical hyperplasia is different with cervical erosion, and cervical intraepithelial neoplasia (CIN) also claims cervical dysplasia, is meant cervical epithelial cells part or most of generation abnormity and atypical differentiation.Cervical dysplasia can betide cervix uteri collar extension or transitional zone or endocervix surface.Carcinoma in situs of cervix is meant that cervical dysplasia involves squama type epithelium holostrome, but does not break through basement membrane, matter between not invading, and pathological changes is confined in the squama type epithelial layer.Cervical intraepithelial neoplasia (CIN) (CIN) patient does not generally show manifest symptom, or the symptom of general cervicitis is only arranged, and as leucorrhoea grow in quantity, a small amount of colporrhagia etc. is arranged also after main suit leucorrhea band blood or the property contact.The visible cervix uteri of gynecologial examination is smooth, does not have obvious inflammation, or has cervix uteri congested or rotten to the corn, and rotten to the corn varying degree, scope are also different, and that touches is easily hemorrhage sometimes, does not have obvious difference with general chronic cervicitis.The concrete typing of cervical intraepithelial neoplasia (CIN) (CIN) is 1. cervical dysplasia-cervical epithelial cells part or most ofly substituted by different heterocysts.Involve scope according to special-shaped degree of cell and epithelium, be divided into light, in, weigh three degree.(1) (CIN I level)-cell atypia is light for slight atypical hyperplasia, and heterocyst only accounts for following 1/3 of epithelial layer; (2) (CIN II level)-cell atypia is obvious for moderate atypical hyperplasia, and heterocyst is limited to following 2/3 of epithelial layer; (3) (CIN III level)-cell atypia is remarkable for the severe atypical hyperplasia, and heterocyst almost involves or all involve epithelial layer.Wherein I level, II level generally can be passed through Drug therapy, and the III level needs operative treatment.
The compound seabuckthorn fruit oil preparation is with prescriptions such as Fructrs Hippophae seed oil, Fructus Cnidii, Radix Sophorae Flavescentis, Myrrhas, has the effect of heat clearing and damp drying, killing parasites for relieving itching, reducing swelling and alleviating pain, blood activating and promoting tissue regeneration.Wherein compound seabuckthorn fruit oil suppository (patent No. is 02114512.1 patent) belongs to national Chinese medicine three kind new medicines, is mainly used in cervical erosion, vaginitis due to the treatment women damp invasion of lower energizer.The applicant is through further experimental study and exploration, discovery compound seabuckthorn fruit oil preparation can not only be used for the treatment of cervical erosion, the vaginitis due to women's damp invasion of lower energizer, also has new medicinal usage, promptly be used for the reparation of wound face behind the cervical erosion naturopathy and cervical intraepithelial neoplasia (CIN) is light, the treatment of moderate atypical hyperplasia, and also relevant therewith so far report.
Summary of the invention:
The object of the present invention is to provide the new purposes of compound seabuckthorn fruit oil in pharmacy, i.e. the purposes of the medicine of light, the moderate atypical hyperplasia of the reparation of compound seabuckthorn fruit oil wound face after preparation is used for the cervical erosion naturopathy and cervical intraepithelial neoplasia (CIN).
Technical scheme of the present invention: the reparation of compound seabuckthorn fruit oil wound face after preparation is used for the cervical erosion naturopathy and cervical intraepithelial neoplasia (CIN) are light, the purposes of the medicine of moderate atypical hyperplasia.
Described compound seabuckthorn fruit oil is the medicament of being made by Fructrs Hippophae seed oil 400-600g, Fructus Cnidii 130-180g, Radix Sophorae Flavescentis 60-100g, Calamina 140-160g, Olibanum 35-60g, Myrrha 35-60g and Borneolum Syntheticum 15-25g.
Preferred compound seabuckthorn fruit oil is the suppository of being made by Fructrs Hippophae seed oil 500g, Fructus Cnidii 150g, Radix Sophorae Flavescentis 80g, Calamina 150g, Olibanum 50g, Myrrha 50g and Borneolum Syntheticum 20g.
Described compound seabuckthorn fruit oil suppository agent is prepared from through following steps: get Fructus Cnidii, Radix Sophorae Flavescentis, Calamina, Olibanum, Myrrha ground spice and be broken into fine powder; Get the Borneolum Syntheticum porphyrize, with above-mentioned powder facing-up, mixing; Other gets glycerol gelatin matrix 1550g, puts heating and melting in 75 ℃ of water-baths, adds the Fructrs Hippophae seed oil and the water 150ml of uniform temp, and emulsifying adds above-mentioned powder to being the thickness glue rapidly, and mixing treats that bubble collapse promptly pours into a mould in the bolt mould, and cooling is granulated, promptly.
The specific embodiment:
To further set forth the present invention by the preparation of compound seabuckthorn fruit oil suppository agent with to the clinical preceding pharmacodynamics test that it carries out below.
One, the preparation of compound seabuckthorn fruit oil suppository
Get Fructus Cnidii 150g, Radix Sophorae Flavescentis 80g, Calamina 150g, Olibanum 50g and Myrrha 50g and be ground into fine powder; Other gets Borneolum Syntheticum 20g porphyrize, with above-mentioned powder facing-up, mixing; Other gets glycerol gelatin matrix 1550g, puts heating and melting in 75 ℃ of water-baths, adds the Fructrs Hippophae seed oil 500g and the water 150ml of uniform temp, and emulsifying is to being the thickness glue rapidly, add above-mentioned powder, mixing treats that bubble collapse promptly pours into a mould in the bolt mould, cooling is granulated, and promptly gets compound seabuckthorn fruit oil suppository.
Usage and dosage: vagina medicinal.Cervical erosion, cervical intraepithelial neoplasia (CIN) begins medication after menstruation is clean; Medication in 3 days behind the cervical erosion naturopathy.Clean external genital, suppository is filled in the vagina depths.1 every night, every day or the next day once, 6 times is a course of treatment.
Two, compound seabuckthorn fruit oil suppository is used for the reparation of wound face behind the cervical erosion naturopathy and cervical intraepithelial neoplasia (CIN) is light, the pharmacodynamic study of moderate atypical hyperplasia
1, for Pharmacodynamic test of active extract before the reparation of wound face behind the cervical erosion naturopathy clinical
1.1 Pyrogentisinic Acid's rubber cement causes the therapeutical effect of rat vagina and cervicitis animal pattern
Experiment material
Animal: the SD female rats, body weight 170-200g, the moving word of quality certification Shan doctor 08-25 number is provided by Shaanxi Chinese medicine academy Experimental Animal Center.
Reagent: 25% phenol rubber cement: liquefied phenol 5ml, Calculus Bovis from Northwest of China have a liking for glue 1g, and glycerol 4ml, adding distil water are to 20ml.
The experiment medicine: compound seabuckthorn fruit oil suppository is provided by Haitian Pharmacy Co., Ltd., Shaanxi, the accurate word Z19991076 of traditional Chinese medicines, lot number 20060612.Specification: every heavy 2.7g.Usage and dosage: vagina administration, 1 every night, 6 times is a course of treatment.
Positive drug: GONGJINGYANKANG SHUAN is provided by Guangxi health pharmaceutcal corporation, Ltd, the accurate word Z20010065 of traditional Chinese medicines, lot number 20060509.Specification: every heavy 1.2g.Usage and dosage: vagina administration, 1 every night, 10 times is a course of treatment.
Experimental technique and result
Get 48 of SD female rats, be divided into blank group at random and (wait the capacity excipient, suitably stop up with cotton balls), model group, positive drug GONGJINGYANKANG SHUAN group (0.4g/kg, clinical 20 times), compound seabuckthorn fruit oil suppository 0.225,0.45,0.9g/kg group (be equivalent to clinical consumption 5,10,20 times), 8 every group.0.1ml/100g body weight phenol rubber cement is injected in every Mus intravaginal, every day 1 time, continuous 3 Cheng Mo, normal group injection equal-volume distilled water, as seen naked eyes go out with the purulence logistics that has that rat vagina lipstick behind the phenol rubber cement swells, has, each treated animal gives corresponding medicine respectively, continuous 7 days from vagina administration (suitably fixing with cotton balls) then.Behind the last medicine 1 hour, vagina was got in conventional anesthesia and cervix uteri is weighed, and calculates organ index, sees Table 1.Get a block organization again and be equipped with inspection, use 10% formalin fixed, carry out histopathology and detect.
The pathological tissue standards of grading:
1) mucosa: 0 minute: do not see obvious degeneration necrosis, come off and ulcer; 1 minute: visible mild degeneration and necrosis, come off and rotten to the corn; 2 minutes: visible obviously degeneration necrosis, come off and ulcer; 3 minutes: visible a large amount of degeneration necrosis, come off and rotten to the corn.
2) tela submucosa vasodilation hyperemia: 0 minute: tela submucosa is not seen obvious blood vessel dilating; 1 minute: the visible slight blood vessel dilating of tela submucosa; 2 minutes: the visible obviously blood vessel dilating of tela submucosa; 3 minutes: the more proliferation of vessels expansion of tela submucosa.
3) tela submucosa cell infiltration: 0 minute: do not see obvious cell infiltration: 1 minute: visible slight cell infiltration; 2 minutes: visible obviously cell infiltration; 3 minutes: visible a large amount of cell infiltration.
4) abscess existence: 0 minute: have abscess to exist; 1 minute: no abscess formed.
The results are shown in Table 2.
The influence of table 1 pair vagina and cervicitis rat model body weight, vagina and cervix uteri animal weight (X ± SD)
Table 2 Pyrogentisinic Acid rubber cement causes the therapeutical effect (X ± SD) of rat vagina and cervicitis model
Annotate: table 1,2 with model control group comparison, * P＜0.05, * * P＜0.01.
As seen table 1 tests front and back rat body weight no significant difference, and the vagina and the cervix uteri organ index of positive group and three dosage groups of compound seabuckthorn fruit oil suppository are starkly lower than model group; Table 2 as seen, mucous epithelium layer pathological changes: the positive, blank, all there are notable difference in big or middle dosed administration group of compound seabuckthorn fruit oil suppository and model group; The proper mucous membrane congestion and edema: the positive, blank, big or middle dosed administration group of compound seabuckthorn fruit oil suppository and model group there is no notable difference; Proper mucous membrane cell infiltration degree: the positive, blank, all there are notable difference in big or middle dosed administration group of compound seabuckthorn fruit oil suppository and model group; Exist abscess to change under the mucosa: the positive, blank, there are notable difference in big or middle dosed administration group of compound seabuckthorn fruit oil suppository and model group.
Brief summary
The result shows that the ponderal index of each dosage group vagina of compound seabuckthorn fruit oil suppository and cervix uteri reduces, and pathology damage takes a turn for the better, and compares P＜0.05～0.01 with model group.Prompting compound seabuckthorn fruit oil suppository Pyrogentisinic Acid rubber cement causes rat vagina and the cervicitis model has certain therapeutical effect.
1.2 staphylococcus aureus is caused the therapeutical effect of vagina and cervicitis animal pattern
Experiment material
Animal: the SD female rats, body weight 170-200g, the moving word of quality certification Shan doctor 08-25 number is provided by Shaanxi Chinese medicine academy Experimental Animal Center.
Staphylococcus aureus: 1,000,000,000 every milliliter.
The experiment medicine: compound seabuckthorn fruit oil suppository is provided by Haitian Pharmacy Co., Ltd., Shaanxi, the accurate word Z19991076 of traditional Chinese medicines, lot number 20060612.Specification: every heavy 2.7g.Usage and dosage: vagina administration, 1 every night, 6 times is a course of treatment.
Positive drug: GONGJINGYANKANG SHUAN is provided by Guangxi health pharmaceutcal corporation, Ltd, the accurate word Z20010065 of traditional Chinese medicines, lot number 20060509.Specification: every heavy 1.2g.Usage and dosage: vagina administration, 1 every night, 10 times is a course of treatment.
Experimental technique and result
Get 48 of SD female rats, be divided into blank group at random and (wait the capacity excipient, suitably stop up with cotton balls), model group, positive drug GONGJINGYANKANG SHUAN group (0.4g/kg, clinical 20 times), compound seabuckthorn fruit oil suppository 0.225,0.45,0.9g/kg group (be equivalent to clinical consumption 5,10,20 times), 8 every group.Every Mus intravaginal mucosa is 2 points of injection down, every some 0.2ml, every day 1 time, continuous 3 Cheng Mo, blank group injection equal-volume normal saline.As seen naked eyes go out with the purulence logistics that has that the rat vagina lipstick of staphylococcus aureus swells, has, and each treated animal gives corresponding medicine respectively, continuous 7 days from vagina administration (suitably fixing with cotton balls) then.Behind the last medicine 1 hour, anesthesia, jugular vein is got blood and is surveyed routine blood test, observes leukocyte and classification, the results are shown in Table 3; Put to death animal, get vagina and the cervix uteri calculating ponderal index of weighing, the results are shown in Table 4; Get a block organization again and be equipped with inspection, use 10% formalin fixed, carry out histopathology and detect, standards of grading the results are shown in Table 5 with test 1.1.
The influence of table 3 pair leukocyte, lymph and neutrophil cell (X ± SD)
The influence of table 4 pair vagina and cervicitis rat model body weight, vagina and cervix uteri animal weight (X ± SD)
Table 5 pair staphylococcus aureus causes the therapeutical effect (X ± SD) of rat vagina and cervicitis model
Annotate: table 3,4,5 with model control group comparison, * P＜0.05, * * P＜0.01.
Table 3 as seen, the total white blood cells of excipient group, positive group and three dosage groups of compound seabuckthorn fruit oil suppository is starkly lower than model group; As seen table 4 tests front and back rat body weight no significant difference, and the vagina and the cervix uteri organ index of positive group and three dosage groups of compound seabuckthorn fruit oil suppository are starkly lower than model group; Table 5 as seen, mucous epithelium layer pathological changes: the positive, blank, all there are notable difference in large, medium and small dosed administration group of compound seabuckthorn fruit oil suppository and model group; The proper mucous membrane congestion and edema: there are notable difference in blank group and model group; Proper mucous membrane cell infiltration degree: the positive, blank, all there are notable difference in large, medium and small dosed administration group of compound seabuckthorn fruit oil suppository and model group; Exist abscess to change under the mucosa: the positive, blank, all there are notable difference in big or middle dosed administration group of compound seabuckthorn fruit oil suppository and model group.
Brief summary
The result shows that each dosage group total white blood cells of compound seabuckthorn fruit oil suppository, vagina and cervix uteri ponderal index reduce, and pathology damage takes a turn for the better, and compares P＜0.05～0.01 with model group.Staphylococcus aureus is caused rat vagina to the prompting compound seabuckthorn fruit oil suppository and cervicitis has certain therapeutical effect.
Pharmacodynamics test to sum up, adopt two kinds of vaginas and cervicitis model to observe compound seabuckthorn fruit oil suppository intravaginal injection staphylococcus aureus is caused the therapeutical effect of rat vagina and cervicitis model and the therapeutical effect that intravaginal injection phenol rubber cement is caused rat vagina and cervicitis model, observed the influence of compound seabuckthorn fruit oil suppository to organ index, histopathology, leukocyte and classification, the result shows that compound seabuckthorn fruit oil suppository is to chemical burn and biotic factor infects actuating thing vagina and the cervicitis model has certain therapeutical effect.The prompting compound seabuckthorn fruit oil suppository is for the clinical preceding effectiveness of cervical erosion treatment.
2, Pharmacodynamic test of active extract before, moderate atypical hyperplasia light clinical for cervical intraepithelial neoplasia (CIN)
Cervical intraepithelial neoplasia (CIN) (CIN) comprises cervical dysplasia and carcinoma in situs of cervix, and the formation of pathological changes is subjected to multiple factor affecting, and individual variation is also very big, is not reported in as yet at present and causes the CIN model on the animal.Consider that compound seabuckthorn fruit oil suppository of the present invention mainly controls light moderate CIN, and light moderate CIN is difficult to distinguish, and generally need only need not deal with by symptomatic treatment clinically with inflammatory hyperplasia.In addition, C1N epithelium of cervix uteri mitosis increases.So the applicant carries out two experiments: (1) is to the mitotic influence test of mouse vagina mucosa basal cell; (2) influence of rat proliferative inflammation is tested.Though can not cause the therapeutical effect of CIN animal model direct observation compound seabuckthorn fruit oil suppository to CIN, above two tests can have suggesting effect to the effectiveness of clinical verification compound seabuckthorn fruit oil suppository.
2.1 to the mitotic influence of mouse vagina mucosa basal cell
Experiment material
Animal: the ICR female mice, body weight 18-22g, the moving word of quality certification Shan doctor 08-24 number is provided by Shaanxi Chinese medicine academy Experimental Animal Center.
Reagent: the diethylstilbestrol injection, Shanghai General Pharmaceutical Co., ltd. produces, lot number 050101.
The experiment medicine: compound seabuckthorn fruit oil suppository is provided by Haitian Pharmacy Co., Ltd., Shaanxi, the accurate word Z19991076 of traditional Chinese medicines, lot number 20060612.Specification: every heavy 2.7g.Usage and dosage: vagina administration, 1 every night, 6 times is a course of treatment.
Positive drug: YUJIN YINXIE PIAN is produced by Xi'an Zhengda Pharmaceutical Co., Ltd., the accurate word Z61021428 of traditional Chinese medicines, lot number 20050813.Specification: the heavy 0.24g of sheet.Usage and dosage: oral, 1 3-6 sheet, 2-3 time on the 1st, people's consumption 0.072g/kg, the bibliographical information YUJIN YINXIE PIAN can suppress mouse vagina mucosa basal cell mitosis.
Select 60 of healthy ICR mices, female, body weight 18-22g is divided into 6 groups at random, 10 every group.First group of normal control group irritated stomach deionized water 10ml/kg, and the two or six group equal lumbar injection diethylstilbestrol 0.2mg/, every day 1 time, for three days on end.And irritate stomach deionization 10ml/kg for second group, be model control group, irritate stomach Radix Curcumae sheet 1.296g/kg for the 3rd group, positive matched group is given compound seabuckthorn fruit oil suppository (suitably fixing with cotton balls) 1.35,0.675,0.3375g/kg (be equivalent to clinical consumption 30,15,7.5 times) from vagina respectively for the four or six group.Continuous 7 days.After the administration on the 7th 4 hours, take off neck and put to death mice, get the mouse vagina tissue, through 10% formalin fixed, draw materials routine paraffin wax embedding, serial section, thickness 5 μ m, HE dyeing is observed with the LEICA biological microscope, the mitosis number that is occurred in 300 mouse vagina basal cells of light microscopic counting, be converted into the mitosis number of per 100 basal cells, see Table 6.
Table 6 pair mouse model vaginal epithelial cell mitosis number influence (X ± SD)
Medicine example number mitosis number
Blank group 10 0.96 ± 1.09**
Model control group 10 6.74 ± 2.57
YUJIN YINXIE PIAN positive controls 10 2.15 ± 1.95**
The heavy dose of group of compound seabuckthorn fruit oil suppository 10 2.22 ± 2.22**
Dosage group 10 2.19 ± 2.33** in the compound seabuckthorn fruit oil suppository
Compound seabuckthorn fruit oil suppository small dose group 10 4.59 ± 2.56
Annotate: compare with model control group, * represents P＜0.05, and * * represents P＜0.01.
Table 6 as seen, mouse model vaginal epithelial cell mitosis number, blank group, YUJIN YINXIE PIAN positive controls, the big or middle dosage group of compound seabuckthorn fruit oil suppository and small dose group all are significantly less than model control group.
Brief summary
The result shows that the big-and-middle dosage of compound seabuckthorn fruit oil suppository can suppress mouse vagina mucosa basal cell mitosis, and the prompting compound seabuckthorn fruit oil suppository has certain mitotic effect of inhibition vaginal mucosa basal cell.
2.2 to the swollen outgrowth influence of rat granuloma
Animal: the SD female rats, body weight 170-200g, the moving word of quality certification Shan doctor 08-25 number is provided by Shaanxi Chinese medicine academy Experimental Animal Center.
Reagent: 25% phenol rubber cement: liquefied phenol 5ml, Calculus Bovis from Northwest of China have a liking for glue 1g, and glycerol 4ml, adding distil water are to 20ml.
The experiment medicine: compound seabuckthorn fruit oil suppository is provided by Haitian Pharmacy Co., Ltd., Shaanxi, the accurate word Z19991076 of traditional Chinese medicines, lot number 20060612.Specification: every heavy 2.7g.Usage and dosage: vagina administration, 1 every night, 6 times is a course of treatment.
Positive drug: GONGJINGYANKANG SHUAN is provided by Guangxi health pharmaceutcal corporation, Ltd, the accurate word Z20010065 of traditional Chinese medicines, lot number 20060509.Specification: every heavy 1.2g.Usage and dosage: vagina administration, 1 every night, 10 times is a course of treatment.
Get 50 of SD rats, be divided into 5 groups, 10 every group.Blank group (wait the capacity excipient, suitably stop up), positive drug GONGJINGYANKANG SHUAN group (0.4g/kg, clinical 20 times), compound seabuckthorn fruit oil suppository 0.225,0.45,0.9g/kg group (be equivalent to clinical consumption 5,10,20 times) with cotton balls.Each is organized rat and all uses etherization, cuts off rat groin place hair, behind iodine tincture and alcohol disinfecting, make a transverse incision, diameter is about 10mm, with vascular forceps skin histology is separated to muscular fasciae, draws back otch gently with tweezers then, 20mg disinfectant cotton balls is imbedded otch, use suturing with thread management two pins at last No. 0, with slip-off preventing, operation intramuscular injection on same day penicillin sodium 4000 units, continuous 4 days, in case infect the beginning administration on the secondth of performing the operation, totally 7 days, the 8th day, with rat haircut sacrificed by exsanguination, with surgical scissors and tweezers granulation tissue to be peeled off, the blood crusts that careful ground will be attached on the granulation is separated with muscular fasciae totally, use scales/electronic balance weighing, gained weight deducts raw cotton ball weight 20mg, promptly gets the granuloma weight in wet base, the results are shown in Table 7, relatively, t checks between group.
The swollen influence that forms of table 7 pair rat granuloma (X ± SD)
Group dosage g/kg number of animals granulation weight in wet base (mg) P value
Matched group-10 414.0 ± 67.19
Positive controls 0.4 10 374.0+49.26＜0.05
Heavy dose of group 0.9 10 314.0 ± 55.22＜0.01
Middle dosage group 0.45 10 332.0 ± 52.45＜0.01
Small dose group 0.225 10 357.0 ± 51.65＜0.05
Table 7 as seen, each dosage of positive drug and compound seabuckthorn fruit oil suppository is formed with obvious inhibitory action to the rat granuloma tissue.
Brief summary
The result shows that the large, medium and small dosage of compound seabuckthorn fruit oil suppository can suppress the rat granuloma tissue and form, and the prompting compound seabuckthorn fruit oil suppository has certain inhibitory action to inflammatory hyperplasia.
Pharmacodynamics test to sum up, the indication newly-increased according to this medicine, adopt two kinds of relevant animal models, observed compound seabuckthorn fruit oil suppository to mouse vagina mucosa basal cell mitotic influence with to the influence of rat proliferative inflammation, the therapeutical effect that the side light compound seabuckthorn fruit oil suppository is light to cervical intraepithelial neoplasia (CIN), moderate atypical hyperplasia is sick, can point out that compound seabuckthorn fruit oil suppository is light for cervical intraepithelial neoplasia (CIN), moderate atypical hyperplasia clinical before effectiveness.
Sum up: pharmacodynamics test to sum up, the indication newly-increased " cervical erosion physics is lived and treated the reparation of back wound face " according to this medicine, adopt two kinds of vaginas and cervicitis model to observe compound seabuckthorn fruit oil suppository intravaginal injection staphylococcus aureus is caused the therapeutical effect of rat vagina and cervicitis model and the therapeutical effect that intravaginal injection phenol rubber cement is caused rat vagina and cervicitis model, observed compound seabuckthorn fruit oil suppository to organ index, histopathology, the influence of leukocyte and classification, the result shows that compound seabuckthorn fruit oil suppository is to chemical burn and biotic factor infects actuating thing vagina and the cervicitis model has certain therapeutical effect.Can point out the clinical preceding effectiveness of compound seabuckthorn fruit oil suppository for the cervical erosion treatment.The indication newly-increased " cervical intraepithelial neoplasia (CIN) is light, moderate atypical hyperplasia " according to this medicine, adopt two kinds of relevant animal models, observed compound seabuckthorn fruit oil suppository to mouse vagina mucosa basal cell mitotic influence with to the influence of rat proliferative inflammation, the therapeutical effect that the side light compound seabuckthorn fruit oil suppository is light to cervical intraepithelial neoplasia (CIN), moderate atypical hyperplasia is sick, can point out that compound seabuckthorn fruit oil suppository is used for that cervical intraepithelial neoplasia (CIN) is light, moderate atypical hyperplasia clinical before effectiveness.
Claims (4)
1. compound seabuckthorn fruit oil is used in preparation that cervical intraepithelial neoplasia (CIN) is light, the purposes of the medicine of moderate atypical hyperplasia.
2. according to the purposes of the described compound seabuckthorn fruit oil of claim 1, it is characterized in that: described compound seabuckthorn fruit oil is the medicament of being made by Fructrs Hippophae seed oil 400-600g, Fructus Cnidii 130-180g, Radix Sophorae Flavescentis 60-100g, Calamina 140-160g, Olibanum 35-60g, Myrrha 35-60g and Borneolum Syntheticum 15-25g.
3. according to the purposes of claim 1 or 2 described compound seabuckthorn fruit oils, it is characterized in that: described compound seabuckthorn fruit oil is the suppository of being made by Fructrs Hippophae seed oil 500g, Fructus Cnidii 150g, Radix Sophorae Flavescentis 80g, Calamina 150g, Olibanum 50g, Myrrha 50g and Borneolum Syntheticum 20g.
4. according to the purposes of the described compound seabuckthorn fruit oil of claim 3, it is characterized in that: described compound seabuckthorn fruit oil suppository agent is prepared from through following steps: get Fructus Cnidii, Radix Sophorae Flavescentis, Calamina, Olibanum, Myrrha ground spice and be broken into fine powder; Get the Borneolum Syntheticum porphyrize, with above-mentioned powder facing-up, mixing; Other gets glycerol gelatin matrix 1550g, puts heating and melting in 75 ℃ of water-baths, adds the Fructrs Hippophae seed oil and the water 150ml of uniform temp, and emulsifying adds above-mentioned powder to being the thickness glue rapidly, and mixing treats that bubble collapse promptly pours into a mould in the bolt mould, and cooling is granulated, promptly.
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