CN104116899A - Preparation method of traditional Chinese medicine washing liquid for treating gynecological inflammation - Google Patents
Preparation method of traditional Chinese medicine washing liquid for treating gynecological inflammation Download PDFInfo
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Abstract
The invention relates to a preparation method of a traditional Chinese medicine washing liquid for treating gynecological inflammation. The washing liquid is prepared by compounding rhizoma anemarrhenae, polygonum cuspidatum, saururus chinensis, lindley eupatorium herb, Chinese magnoliavine fruit, licorice, golden larch bark, catechu, desmodium, and common carpesium fruit. The prepared washing liquid is a broad-spectrum high-effective contact bactericide, will not generate drug resistance, can promote the synthesis of vaginal mucosa epithelial cells and glycogen secretion, promote the generation of vaginal lactobacillus, and recover the normal ecological system of vagina, and can be used to treat colpomycosis and common colpitis.
Description
Technical field
The present invention relates to technical field of Chinese medicines, be specifically related to a kind of preparation method for the treatment of the Effect of Chinese Medicine Qing washing liquid of gynecological inflammation.
Background technology
Vaginitis in gynaecopathia, pelvic inflammatory disease, cervicitis, cervical erosion are a kind of commonly encountered diseases and frequently-occurring disease, and the Therapeutic Method for these gynaecopathias mainly adopts physiotherapy and Drug therapy at present.The conventional means of naturopathy is a little scalded art, laser therapy and cryotherapy, and its ultimate principle is caused inflammation tissue necrosis, come off by physical factor exactly, then in cervix uteri, again grows squamous epithelial tissue.Though physiotherapy can make inflammatory tissue come off, cervical mucosa is newborn to be repaired, and has the side effect such as hemorrhage, water sample oozes out, and also can shrink and make uterus because of lymphoid tissue
Neck tube is narrow, adhesion, and must treat in hospital, expense high and trouble.In Drug therapy except oral medicine, also has outer therapy, outer therapy is mainly with Chinese medicine fumigation and washing, suppository, effervescent etc. are treated, although there is certain effect, in therapeutic process, patient feels cumbersome (as fumigation and wash method), while adopting suppository in treatment, person feels foreign body sensation can to make uterine erosion, and while utilizing effervescent treatment, patient can feel again dry uncomfortable.These phenomenons are all because the factor of dosage form is brought bad reaction, make patient be unwilling to adhere to treatment.
Summary of the invention
The object of the invention is the deficiency for solving the problems of the technologies described above, a kind of preparation method for the treatment of the Effect of Chinese Medicine Qing washing liquid of gynecological inflammation is provided, the lotion product making can effectively be treated the gynecological inflammation of middle aged and aged women.
The present invention is the deficiency solving the problems of the technologies described above, and the technical scheme adopting is: a kind of preparation method for the treatment of the Effect of Chinese Medicine Qing washing liquid of gynecological inflammation, comprises the following steps:
(1) get the Rhizoma Anemarrhenae, alcohol reflux by 20 times of weight, extract altogether twice, each 2h, merge the ethanol extract of twice, filter, reclaim ethanol, concentrated alcohol is sink to containing alcohol 80%, reclaim ethanol, be diluted with water to 10 times of medical material weight, obtain medicinal liquid, then regulating PH is 4, and medicinal liquid is crossed to anion exchange resin, medicinal liquid has been crossed and washed post bed to effluent with water after post bed is colourless, it is colourless with 0.8% NaOH, being eluted to effluent again, collect alkali eluent, adjusting pH value is 5, filter, water bath method, at 80 ℃ of temperature, dry, obtain powder I, the weight ratio of take is again mixed as 10:1 gets powder I and osmanthus flower fragrance, stir, obtain mixed powder I, standby,
(2) get Rhizoma Polygoni Cuspidati, pulverized 50 orders, then adding mass concentration is 3% calcium hydroxide spice, then adds the water of 10 times to heat to 60 ℃, is incubated 2 hours, filters, and obtains extracting solution; Regulate the pH value to 9 of extracting solution, upper macroporous adsorbent resin after filtering, adsorb, adsorb saturated after washing, cylinder filler after washing is first used 0.3% sodium hydroxide eluting, do not collect eluent, after with containing volumetric concentration, be the eluent eluting that 60% alcoholic solution and mass concentration are 1% sodium hydroxide again, collecting eluent, is 2 rear centrifugal by eluent with sulphuric acid adjust pH, and cold drying obtains powder II, the weight ratio of take is again mixed as 12:1 gets powder II and vanillin, stir, obtain mixed powder II, standby;
(3) get the rhizome of Rhizoma Saururi (Herba Saururi), the methanol that adds 8 times of weight after pulverizing, heating and refluxing extraction 2h, filtering residue adds the methanol of 6 times of weight again, and heating and refluxing extraction 1h filters, merge filtrate twice, vacuum concentration obtains extractum, then with the hot water of 3 times of extractum weight, extractum is spread, then with isopyknic ethyl acetate extraction 4 times, extract is after reclaim under reduced pressure ethyl acetate, obtain extractum, vacuum becomes dry powder, obtains powder III, the weight ratio of take is again mixed as 15:1 gets powder III and gardenia compound, stir, obtain mixed powder III, standby;
(4) according to parts by weight get mixed powder I 30-50 part, mixed powder II 20-40 part,
Mixed powder III 20-40 part, Herba Eupatorii Lindleyani 50-80 part, Fructus Schisandrae Chinensis 40-60 part, Radix Glycyrrhizae 40-60 part, Cortex Pseudolaricis 10-20 part, catechu 15-20 part, Herba Lysimachiae 10-20 part, Fructus Carpesii 10-20 part and Alumen 40-60 part are standby;
(5) get Herba Eupatorii Lindleyani, Fructus Schisandrae Chinensis, Radix Glycyrrhizae, Cortex Pseudolaricis, catechu, Herba Lysimachiae and Fructus Carpesii, be placed in multi-function extractor, add 8 times to the drinking-water of drug weight, heating extraction 1 hour, filters; Add again 6 times of water gagings and extract 1 hour, filter; Add 4 times of water gagings again and extract 0.5 hour, filter, merging filtrate, makes extracting solution, then the mixed powder I of taking-up, mixed powder II and mixed powder III is added in extracting solution, stirs 5-10min, obtains mixed extract;
(6) above-mentioned mixed extract is placed in settling tank, adds Alumen to dissolve, staticly settle 14 hours, then get the supernatant of the medicinal liquid after precipitation, be placed in high speed centrifuge, carry out centrifugal filtration; After filtering, filtrate is put in concentrating under reduced pressure tank, through concentrating under reduced pressure, is concentrated into relative density to 1.25, takes out concentrated solution and is transported in Agitation Tank; By concentrated solution in Agitation Tank, adjust relative density to 1.04, add the sodium benzoate of liquor capacity 0.3% simultaneously, take out medicinal liquid, put clean container, make the colpitic lotion for the treatment of.
The addition of the middle calcium hydroxide of described step (2) is 0.1 times of Rhizoma Polygoni Cuspidati weight.
The usage of cleanout fluid of the present invention and consumption: depend on patient's the factor such as age, body weight, health status, as guidance: ampoule 100ml, add warm water to 1000ml, 1 time on the one 1 week is 1 course for the treatment of.
beneficial effect
Cleanout fluid of the present invention is by the Rhizoma Anemarrhenae, Rhizoma Polygoni Cuspidati, Rhizoma Saururi (Herba Saururi), Herba Eupatorii Lindleyani, Fructus Schisandrae Chinensis, Radix Glycyrrhizae, Cortex Pseudolaricis, catechu, Herba Lysimachiae and Fructus Carpesii compatibility, the contact antibacterial of the lotion broad-spectrum high efficacy making, and do not form Drug resistance, can promote that vaginal epithelium is synthetic and secrete glycogen, promote the generation of vagina lactobacillus, recover the normal ecosystem of vagina, can be used for the treatment of colpitis mycotica and general vaginitis.
The specific embodiment
embodiment 1:
A preparation method for the treatment of the Effect of Chinese Medicine Qing washing liquid of gynecological inflammation, comprises the following steps:
(1) get the Rhizoma Anemarrhenae, alcohol reflux by 20 times of weight, extract altogether twice, each 2h, merge the ethanol extract of twice, filter, reclaim ethanol, concentrated alcohol is sink to containing alcohol 80%, reclaim ethanol, be diluted with water to 10 times of medical material weight, obtain medicinal liquid, then regulating PH is 4, and medicinal liquid is crossed to anion exchange resin, medicinal liquid has been crossed and washed post bed to effluent with water after post bed is colourless, it is colourless with 0.8% NaOH, being eluted to effluent again, collect alkali eluent, adjusting pH value is 5, filter, water bath method, at 80 ℃ of temperature, dry, obtain powder I, the weight ratio of take is again mixed as 10:1 gets powder I and osmanthus flower fragrance, stir, obtain mixed powder I, standby,
(2) get Rhizoma Polygoni Cuspidati, pulverized 50 orders, then adding mass concentration is 3% calcium hydroxide spice (in described step (2), the addition of calcium hydroxide is 0.1 times of Rhizoma Polygoni Cuspidati weight), then add the water of 10 times to heat to 60 ℃, be incubated 2 hours, filter, obtain extracting solution; Regulate the pH value to 9 of extracting solution, upper macroporous adsorbent resin after filtering, adsorb, adsorb saturated after washing, cylinder filler after washing is first used 0.3% sodium hydroxide eluting, do not collect eluent, after with containing volumetric concentration, be the eluent eluting that 60% alcoholic solution and mass concentration are 1% sodium hydroxide again, collecting eluent, is 2 rear centrifugal by eluent with sulphuric acid adjust pH, and cold drying obtains powder II, the weight ratio of take is again mixed as 12:1 gets powder II and vanillin, stir, obtain mixed powder II, standby;
(3) get the rhizome of Rhizoma Saururi (Herba Saururi), the methanol that adds 8 times of weight after pulverizing, heating and refluxing extraction 2h, filtering residue adds the methanol of 6 times of weight again, and heating and refluxing extraction 1h filters, merge filtrate twice, vacuum concentration obtains extractum, then with the hot water of 3 times of extractum weight, extractum is spread, then with isopyknic ethyl acetate extraction 4 times, extract is after reclaim under reduced pressure ethyl acetate, obtain extractum, vacuum becomes dry powder, obtains powder III, the weight ratio of take is again mixed as 15:1 gets powder III and gardenia compound, stir, obtain mixed powder III, standby;
(4) according to parts by weight get 30 parts of mixed powder I, 20 parts of mixed powder II,
40 parts, 20 parts of mixed powder III, 50 parts of Herba Eupatorii Lindleyanis, 40 parts of Fructus Schisandrae Chinensis, 40 parts, Radix Glycyrrhizae, 10 parts of Cortex Pseudolariciss, 15 parts, catechu, 10 parts of Herba Lysimachiaes, 10 parts of Fructus Carpesiis and Alumen, standby;
(5) get Herba Eupatorii Lindleyani, Fructus Schisandrae Chinensis, Radix Glycyrrhizae, Cortex Pseudolaricis, catechu, Herba Lysimachiae and Fructus Carpesii, be placed in multi-function extractor, add 8 times to the drinking-water of drug weight, heating extraction 1 hour, filters; Add again 6 times of water gagings and extract 1 hour, filter; Add 4 times of water gagings again and extract 0.5 hour, filter, merging filtrate, makes extracting solution, then the mixed powder I of taking-up, mixed powder II and mixed powder III is added in extracting solution, stirs 5-10min, obtains mixed extract;
(6) above-mentioned mixed extract is placed in settling tank, adds Alumen to dissolve, staticly settle 14 hours, then get the supernatant of the medicinal liquid after precipitation, be placed in high speed centrifuge, carry out centrifugal filtration; After filtering, filtrate is put in concentrating under reduced pressure tank, through concentrating under reduced pressure, is concentrated into relative density to 1.25, takes out concentrated solution and is transported in Agitation Tank; By concentrated solution in Agitation Tank, adjust relative density to 1.04, add the sodium benzoate of liquor capacity 0.3% simultaneously, take out medicinal liquid, put clean container, make the colpitic lotion for the treatment of.
embodiment 2:
A preparation method for the treatment of the Effect of Chinese Medicine Qing washing liquid of gynecological inflammation, comprises the following steps:
(1) get the Rhizoma Anemarrhenae, alcohol reflux by 20 times of weight, extract altogether twice, each 2h, merge the ethanol extract of twice, filter, reclaim ethanol, concentrated alcohol is sink to containing alcohol 80%, reclaim ethanol, be diluted with water to 10 times of medical material weight, obtain medicinal liquid, then regulating PH is 4, and medicinal liquid is crossed to anion exchange resin, medicinal liquid has been crossed and washed post bed to effluent with water after post bed is colourless, it is colourless with 0.8% NaOH, being eluted to effluent again, collect alkali eluent, adjusting pH value is 5, filter, water bath method, at 80 ℃ of temperature, dry, obtain powder I, the weight ratio of take is again mixed as 10:1 gets powder I and osmanthus flower fragrance, stir, obtain mixed powder I, standby,
(2) get Rhizoma Polygoni Cuspidati, pulverized 50 orders, then adding mass concentration is 3% calcium hydroxide spice (in described step (2), the addition of calcium hydroxide is 0.1 times of Rhizoma Polygoni Cuspidati weight), then add the water of 10 times to heat to 60 ℃, be incubated 2 hours, filter, obtain extracting solution; Regulate the pH value to 9 of extracting solution, upper macroporous adsorbent resin after filtering, adsorb, adsorb saturated after washing, cylinder filler after washing is first used 0.3% sodium hydroxide eluting, do not collect eluent, after with containing volumetric concentration, be the eluent eluting that 60% alcoholic solution and mass concentration are 1% sodium hydroxide again, collecting eluent, is 2 rear centrifugal by eluent with sulphuric acid adjust pH, and cold drying obtains powder II, the weight ratio of take is again mixed as 12:1 gets powder II and vanillin, stir, obtain mixed powder II, standby;
(3) get the rhizome of Rhizoma Saururi (Herba Saururi), the methanol that adds 8 times of weight after pulverizing, heating and refluxing extraction 2h, filtering residue adds the methanol of 6 times of weight again, and heating and refluxing extraction 1h filters, merge filtrate twice, vacuum concentration obtains extractum, then with the hot water of 3 times of extractum weight, extractum is spread, then with isopyknic ethyl acetate extraction 4 times, extract is after reclaim under reduced pressure ethyl acetate, obtain extractum, vacuum becomes dry powder, obtains powder III, the weight ratio of take is again mixed as 15:1 gets powder III and gardenia compound, stir, obtain mixed powder III, standby;
(4) according to parts by weight, get 60 parts, 50 parts of mixed powder I, 40 parts of mixed powder II, 40 parts of mixed powder III, 80 parts of Herba Eupatorii Lindleyanis, 60 parts of Fructus Schisandrae Chinensis, 60 parts, Radix Glycyrrhizae, 20 parts of Cortex Pseudolariciss, 20 parts, catechu, 20 parts of Herba Lysimachiaes, 20 parts of Fructus Carpesiis and Alumen, standby;
(5) get Herba Eupatorii Lindleyani, Fructus Schisandrae Chinensis, Radix Glycyrrhizae, Cortex Pseudolaricis, catechu, Herba Lysimachiae and Fructus Carpesii, be placed in multi-function extractor, add 8 times to the drinking-water of drug weight, heating extraction 1 hour, filters; Add again 6 times of water gagings and extract 1 hour, filter; Add 4 times of water gagings again and extract 0.5 hour, filter, merging filtrate, makes extracting solution, then the mixed powder I of taking-up, mixed powder II and mixed powder III is added in extracting solution, stirs 5-10min, obtains mixed extract;
(6) above-mentioned mixed extract is placed in settling tank, adds Alumen to dissolve, staticly settle 14 hours, then get the supernatant of the medicinal liquid after precipitation, be placed in high speed centrifuge, carry out centrifugal filtration; After filtering, filtrate is put in concentrating under reduced pressure tank, through concentrating under reduced pressure, is concentrated into relative density to 1.25, takes out concentrated solution and is transported in Agitation Tank; By concentrated solution in Agitation Tank, adjust relative density to 1.04, add the sodium benzoate of liquor capacity 0.3% simultaneously, take out medicinal liquid, put clean container, make the colpitic lotion for the treatment of.
embodiment 3:
A preparation method for the treatment of the Effect of Chinese Medicine Qing washing liquid of gynecological inflammation, comprises the following steps:
(1) get the Rhizoma Anemarrhenae, alcohol reflux by 20 times of weight, extract altogether twice, each 2h, merge the ethanol extract of twice, filter, reclaim ethanol, concentrated alcohol is sink to containing alcohol 80%, reclaim ethanol, be diluted with water to 10 times of medical material weight, obtain medicinal liquid, then regulating PH is 4, and medicinal liquid is crossed to anion exchange resin, medicinal liquid has been crossed and washed post bed to effluent with water after post bed is colourless, it is colourless with 0.8% NaOH, being eluted to effluent again, collect alkali eluent, adjusting pH value is 5, filter, water bath method, at 80 ℃ of temperature, dry, obtain powder I, the weight ratio of take is again mixed as 10:1 gets powder I and osmanthus flower fragrance, stir, obtain mixed powder I, standby,
(2) get Rhizoma Polygoni Cuspidati, pulverized 50 orders, then adding mass concentration is 3% calcium hydroxide spice (in described step (2), the addition of calcium hydroxide is 0.1 times of Rhizoma Polygoni Cuspidati weight), then add the water of 10 times to heat to 60 ℃, be incubated 2 hours, filter, obtain extracting solution; Regulate the pH value to 9 of extracting solution, upper macroporous adsorbent resin after filtering, adsorb, adsorb saturated after washing, cylinder filler after washing is first used 0.3% sodium hydroxide eluting, do not collect eluent, after with containing volumetric concentration, be the eluent eluting that 60% alcoholic solution and mass concentration are 1% sodium hydroxide again, collecting eluent, is 2 rear centrifugal by eluent with sulphuric acid adjust pH, and cold drying obtains powder II, the weight ratio of take is again mixed as 12:1 gets powder II and vanillin, stir, obtain mixed powder II, standby;
(3) get the rhizome of Rhizoma Saururi (Herba Saururi), the methanol that adds 8 times of weight after pulverizing, heating and refluxing extraction 2h, filtering residue adds the methanol of 6 times of weight again, and heating and refluxing extraction 1h filters, merge filtrate twice, vacuum concentration obtains extractum, then with the hot water of 3 times of extractum weight, extractum is spread, then with isopyknic ethyl acetate extraction 4 times, extract is after reclaim under reduced pressure ethyl acetate, obtain extractum, vacuum becomes dry powder, obtains powder III, the weight ratio of take is again mixed as 15:1 gets powder III and gardenia compound, stir, obtain mixed powder III, standby;
(4) according to parts by weight, get 50 parts, 40 parts of mixed powder I, 30 parts of mixed powder II, 30 parts of mixed powder III, 60 parts of Herba Eupatorii Lindleyanis, 50 parts of Fructus Schisandrae Chinensis, 50 parts, Radix Glycyrrhizae, 15 parts of Cortex Pseudolariciss, 17 parts, catechu, 15 parts of Herba Lysimachiaes, 15 parts of Fructus Carpesiis and Alumen, standby;
(5) get Herba Eupatorii Lindleyani, Fructus Schisandrae Chinensis, Radix Glycyrrhizae, Cortex Pseudolaricis, catechu, Herba Lysimachiae and Fructus Carpesii, be placed in multi-function extractor, add 8 times to the drinking-water of drug weight, heating extraction 1 hour, filters; Add again 6 times of water gagings and extract 1 hour, filter; Add 4 times of water gagings again and extract 0.5 hour, filter, merging filtrate, makes extracting solution, then the mixed powder I of taking-up, mixed powder II and mixed powder III is added in extracting solution, stirs 5-10min, obtains mixed extract;
(6) above-mentioned mixed extract is placed in settling tank, adds Alumen to dissolve, staticly settle 14 hours, then get the supernatant of the medicinal liquid after precipitation, be placed in high speed centrifuge, carry out centrifugal filtration; After filtering, filtrate is put in concentrating under reduced pressure tank, through concentrating under reduced pressure, is concentrated into relative density to 1.25, takes out concentrated solution and is transported in Agitation Tank; By concentrated solution in Agitation Tank, adjust relative density to 1.04, add the sodium benzoate of liquor capacity 0.3% simultaneously, take out medicinal liquid, put clean container, make the colpitic lotion for the treatment of.
test the therapeutic effect of lotion of the present invention to rat vagina inflammation
Adopt the not healthy rat of copulation of 40 female, sexual maturity, body weight is 250 ± 20g.
Test group medication is the prepared Chinese medicine lotion of embodiment of the present invention l; Positive drug control group medication is JIEERYIN XIYE, from hospital or pharmacy, buys.
Experimental strain: staphylococcus aureus and escherichia coli plastc ring concentration: l09cfu/mL, i.e. staphylococcus aureus bacterium; Suspension concentration is 5 * l08cfulmL, and escherichia coli bacteria suspension concentration is 5 * l08cfulmL.
test method
1, by normal female mouse carotid back subcutaneous injection estradiol benzoate injection 2mg/mLO. 05mL, continuous 3 times, 2 days 1 time.In the 6th day, staphylococcus aureus and the uncommon mattress plastc ring 20uL of large intestine dust are injected to operation absorption styptic sponge, the hands wood absorption styptic sponge plug that oneself is injected to strain is in mouse vagina, in postvaccinal the 4th day, get vaginal secretions smear for microscopic examination, the growing state of observing mycelia and blastopore, infects successfully to determine.
2, rat is fed l after week in adaptability, is divided at random 4 groups, 10 every group, is respectively: blank group, model group, positive drug control group, test group.Model group and blank group are given respectively normal saline, and positive drug control group is given with JIEERYIN XIYE, test group
Chinese medicine lotion of the present invention, all administration is 10 days, and medication is that medicinal liquid is injected in rat vagina.After model group and each administration group rat inoculation bacterium, observe day by day, blank group rat is inoculated bacteria not, and except blank group, model group and each administration group rat all present vaginitis symptom after injecting antibacterial 5d.After there is typical vaginitis symptom, each administration group gives respectively Drug therapy.Blank group gives intravaginal saline injection, and successive administration lOd is a course for the treatment of, every day 1 time.
observation index
Rat vagina secretions is measured: within the 1st, 4 days after last administration, get vaginal swab smear for microscopic examination, 2 microscopy results are negative patient and turn out cloudy for this pathogen, to determine its situation of turning out cloudy.
Pathological study: get vagina tissue section after execution rat and do pathological examination, be divided into 0-111 level by pathology, 0 grade is that mucosal epithelium is smooth, comes off without necrosis; Nothing hyperemia, edema, hemorrhage under mucosa, scorching delicate infiltration; Intravaginal is without inflammatory exudate.I level is that mucosal epithelium comes off without necrosis; Congested, edema under mucosa; Intravaginal is without inflammatory exudate.II level is that mucosal epithelium has focal necrosis to come off; Hyperemia under mucosa, edema, petechial hemorrhage, a small amount of cell infiltration; Intravaginal has a small amount of inflammatory exudate.III level is that mucosal epithelium necrosis comes off; Hyperemia under mucosa, edema, lamellar are hemorrhage, cell infiltration; Intravaginal has a large amount of inflammatory exudates.
Result is as follows:
Table 1 is the impact of each group on rat vagina secretions
Blank treated animal vaginal secretion microscopy is all negative, and with model control group comparison, difference has statistical significance (P<0.05); Prompting bacterial vaginitis mouse model modeling success; After test group, positive controls drug withdrawal animal vaginal secretion microscopy staphylococcus aureus turn out cloudy number and escherichia coli turn out cloudy number respectively with model group comparison, difference has statistical significance (P<0.05); But compare and there is no statistical significance between test group and positive drug control group.
Table 2 is the impact of each group on rat vagina secretions
Grade situation and the model control group comparison of blank treated animal vaginal infection specimen histopathologic examination, difference has statistical significance; Test group, positive want grade situation and the model control group comparison of control animals vaginal infection specimen histopathologic examination, difference has statistical significance (P<0.05). but compare and there is no statistical significance between test group and positive drug control group.Animal test results shows: the lotion that the present invention makes all has obvious therapeutical effect to rat bacterial vaginitis, and animal vaginal mucosa tissue is had to significant protective effect.
human clinical trial's data of lotion of the present invention
Select in May, 2012 in December ,-20 13 to be diagnosed as atrophic vaginitis 106 examples, 49 years old～69 years old age, diagnosis mark Huaihe River: with reference to < < obstetrics and gynecology > > (the 5th edition), all cases is all clarified a diagnosis according to medical history, gynecologial examination.Symptom is xerosis vulae, pale or the flushing of vaginal mucosa or have little petechia, vaginal health scoring <15 divides, serum follicle stimulating hormone (FSH) >=40 work U/L, serum estradiol (E2)≤llOpmol/L, abdominal ultrasound examination endometrium thickness
<5mm。All patients all carry out vaginal secretions inspection and get rid of trichomonal vaginitis, monilial vaginitis before treatment, and urine is cultivated negative, without vulvar dystrophy and dermatosis.All cases are got rid of hormone-dependent tumor, do not accept hormone medicine treatment over nearly 3 months.
Matched group 53 example and test group 53 examples compare before two groups of patient treatments in age, the state of an illness, the course of disease, and difference not statistically significant (P>0.05), has comparability.
Matched group adopts 1% lactic acid solution washout, the red and swollen significantly local antibiotic that uses of pudendum
Emulsion, 1 time/d.The lotion washout that test group makes with the embodiment of the present invention 1,1 ld.
Observation index: conventional inquiry medical history before and after medication, and according to vaginal health point system (VHS) scoring, determine symptom improvement situation.
Criterion of therapeutical effect: with reference to < < obstetrics and gynecology > >, recovery from illness: clinical condition is held up, sign disappears; Effective: clinical symptoms, sign turn for the better by and large; Effective: clinical symptoms, sign alleviate; Invalid: clinical symptoms, sign are unchanged.
Statistical analysis: SPSS13.0 carries out statistical analysis.Vaginal health scoring single index adopts nonparametric statistics, and measurement data is checked with t, and enumeration data is checked with X2.
Result of the test:
Vaginal symptoms is improved situation: after medication 5d, and the patient of matched group 66.03%, and all case vagina drying of test group, dyspareunia symptom all start to obtain improvement in various degree.Gynecologial examination vaginal wall elasticity increases, and fold is deepened, and surface is pale, flushing and bleeding are clearly better.According to vaginal health standards of grading, the summation that vaginal health scoring is elasticity, wettability, pH, mucosa, 5 project score values of secretions.Before test group patient's medication, scoring is 8.27 ± 1.74, and the average of marking after medication 14d is 14.26 ± 1.93.
Two groups of Comprehensive Clinical curative effects comparison: after medication 14d, matched group cure rate, obvious effective rate, effective percentage, inefficiency are about respectively 20%, 35%, 35%, 10%; The cure rate of test group, obvious effective rate, effective percentage, inefficiency are respectively 60%, 30%, 10%, 0.00%.
This result of study shows, lotion for treatment atrophic vaginitis total effective rate of the present invention is 100%, has that untoward reaction is light, the higher advantage of safety, and in 3 months, relapse rate is low.
Claims (2)
1. a preparation method for the treatment of the Effect of Chinese Medicine Qing washing liquid of gynecological inflammation, is characterized in that: comprise the following steps:
(1) get the Rhizoma Anemarrhenae, alcohol reflux by 20 times of weight, extract altogether twice, each 2h, merge the ethanol extract of twice, filter, reclaim ethanol, concentrated alcohol is sink to containing alcohol 80%, reclaim ethanol, be diluted with water to 10 times of medical material weight, obtain medicinal liquid, then regulating PH is 4, and medicinal liquid is crossed to anion exchange resin, medicinal liquid has been crossed and washed post bed to effluent with water after post bed is colourless, it is colourless with 0.8% NaOH, being eluted to effluent again, collect alkali eluent, adjusting pH value is 5, filter, water bath method, at 80 ℃ of temperature, dry, obtain powder I, the weight ratio of take is again mixed as 10:1 gets powder I and osmanthus flower fragrance, stir, obtain mixed powder I, standby,
(2) get Rhizoma Polygoni Cuspidati, pulverized 50 orders, then adding mass concentration is 3% calcium hydroxide spice, then adds the water of 10 times to heat to 60 ℃, is incubated 2 hours, filters, and obtains extracting solution; Regulate the pH value to 9 of extracting solution, upper macroporous adsorbent resin after filtering, adsorb, adsorb saturated after washing, cylinder filler after washing is first used 0.3% sodium hydroxide eluting, do not collect eluent, after with containing volumetric concentration, be the eluent eluting that 60% alcoholic solution and mass concentration are 1% sodium hydroxide again, collecting eluent, is 2 rear centrifugal by eluent with sulphuric acid adjust pH, and cold drying obtains powder II, the weight ratio of take is again mixed as 12:1 gets powder II and vanillin, stir, obtain mixed powder II, standby;
(3) get the rhizome of Rhizoma Saururi (Herba Saururi), the methanol that adds 8 times of weight after pulverizing, heating and refluxing extraction 2h, filtering residue adds the methanol of 6 times of weight again, and heating and refluxing extraction 1h filters, merge filtrate twice, vacuum concentration obtains extractum, then with the hot water of 3 times of extractum weight, extractum is spread, then with isopyknic ethyl acetate extraction 4 times, extract is after reclaim under reduced pressure ethyl acetate, obtain extractum, vacuum becomes dry powder, obtains powder III, the weight ratio of take is again mixed as 15:1 gets powder III and gardenia compound, stir, obtain mixed powder III, standby;
(4) according to parts by weight get mixed powder I 30-50 part, mixed powder II 20-40 part,
Mixed powder III 20-40 part, Herba Eupatorii Lindleyani 50-80 part, Fructus Schisandrae Chinensis 40-60 part, Radix Glycyrrhizae 40-60 part, Cortex Pseudolaricis 10-20 part, catechu 15-20 part, Herba Lysimachiae 10-20 part, Fructus Carpesii 10-20 part and Alumen 40-60 part are standby;
(5) get Herba Eupatorii Lindleyani, Fructus Schisandrae Chinensis, Radix Glycyrrhizae, Cortex Pseudolaricis, catechu, Herba Lysimachiae and Fructus Carpesii, be placed in multi-function extractor, add 8 times to the drinking-water of drug weight, heating extraction 1 hour, filters; Add again 6 times of water gagings and extract 1 hour, filter; Add 4 times of water gagings again and extract 0.5 hour, filter, merging filtrate, makes extracting solution, then the mixed powder I of taking-up, mixed powder II and mixed powder III is added in extracting solution, stirs 5-10min, obtains mixed extract;
(6) above-mentioned mixed extract is placed in settling tank, adds Alumen to dissolve, staticly settle 14 hours, then get the supernatant of the medicinal liquid after precipitation, be placed in high speed centrifuge, carry out centrifugal filtration; After filtering, filtrate is put in concentrating under reduced pressure tank, through concentrating under reduced pressure, is concentrated into relative density to 1.25, takes out concentrated solution and is transported in Agitation Tank; By concentrated solution in Agitation Tank, adjust relative density to 1.04, add the sodium benzoate of liquor capacity 0.3% simultaneously, take out medicinal liquid, put clean container, make the colpitic lotion for the treatment of.
2. a kind of preparation method for the treatment of the Effect of Chinese Medicine Qing washing liquid of gynecological inflammation as claimed in claim 1, is characterized in that: the addition of the middle calcium hydroxide of described step (2) is 0.1 times of Rhizoma Polygoni Cuspidati weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410338138.2A CN104116899A (en) | 2014-07-16 | 2014-07-16 | Preparation method of traditional Chinese medicine washing liquid for treating gynecological inflammation |
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| CN110123703A (en) * | 2019-06-17 | 2019-08-16 | 广州市百爱神生物科技有限公司 | One kind resisting antibacterial nursing cleaning curing agent and preparation method for gynaecology's secret |
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| CN1879831A (en) * | 2006-05-17 | 2006-12-20 | 杨晋懿 | Bacteriostatic externally applied preparation for treating gynecological inflammation and method for preparing same |
| CN102743609A (en) * | 2012-06-17 | 2012-10-24 | 隗淑新 | Preparation method of Chinese medicine irrigation solution for treating vagina mucosa swelling type mycotic vaginitis |
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| CN1879831A (en) * | 2006-05-17 | 2006-12-20 | 杨晋懿 | Bacteriostatic externally applied preparation for treating gynecological inflammation and method for preparing same |
| CN102743609A (en) * | 2012-06-17 | 2012-10-24 | 隗淑新 | Preparation method of Chinese medicine irrigation solution for treating vagina mucosa swelling type mycotic vaginitis |
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| 宋小妹主编: "《中药化学》", 31 August 2012 * |
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| CN110123703A (en) * | 2019-06-17 | 2019-08-16 | 广州市百爱神生物科技有限公司 | One kind resisting antibacterial nursing cleaning curing agent and preparation method for gynaecology's secret |
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Application publication date: 20141029 |