CN101215558A - 一种全血dna的提取方法 - Google Patents

一种全血dna的提取方法 Download PDF

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CN101215558A
CN101215558A CNA2008100137776A CN200810013777A CN101215558A CN 101215558 A CN101215558 A CN 101215558A CN A2008100137776 A CNA2008100137776 A CN A2008100137776A CN 200810013777 A CN200810013777 A CN 200810013777A CN 101215558 A CN101215558 A CN 101215558A
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dna
centrifuge tube
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陈刚
杨树林
温晓燕
林汝湘
刘文敏
陈星�
周鹏
魏然
朱海宁
栾萌
王博
高春义
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陈刚
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Abstract

本发明公开了一种全血DNA的提取方法，解决了目前提取全血DNA时提取率不高的缺点，本发明的步骤为：将血置于离心管中，加入含有蛋白酶K的缓冲液进行酶切，离心，加入酚，再离心，然后用酚－氯仿混合物提取2～3次，即得DNA；本发明所用的蛋白酶K的缓冲液为含有20mmol/L Tris.Cl(pH8.0)、200mmol/L EDTA(pH8.0)、1％SDS、240ug/ml蛋白酶K的水溶液。本发明具有简单、有效、提取率高等优点。

Description

一种全血DNA的提取方法
技术领域
本发明涉及一种全血DNA的提取方法。
背景技术
目前，提取全血DNA的方法一般为：首先用红细胞裂解液破碎红细胞，然后通过离心使白细胞沉淀，然后反复洗涤以弃去血红蛋白，虽然这样能提取出纯度较高的DNA，但是在破碎、离心过程中有相当数量的白细胞亦遭受破坏，尤其是冻存多年的陈旧血液；由于数量不等的白细胞随着血红蛋白一起被丢弃，因而造成了DNA的大量损失；有些血液样本资源十分宝贵，来源有限，不可再生，丢失会造成无可估量向的损失，其后果不可挽回。因此，急需一种既能保证纯度、提取率又高的方法来提取全血DNA。
发明内容
针对上述现有技术的不足，本发明提供了一种提取率高的全血DNA的提取方法。
本发明是通过以下技术方案实现的：
一种全血DNA高产出率的提取方法，包括以下步骤：
(1).取冻融处理的ACD抗凝血，置于离心试管中，加入含有240ug/ml蛋白酶K的缓冲液，振荡混匀，置于50℃水浴20～28小时，水浴过程中缓慢摇动离心管；
(2).酶切完成后，向离心管中加入等体积的pH为8.0的重蒸饱和酚，盖紧管盖，反复颠倒或用DNA混合仪转动混匀，直至两相混合均匀；
(3).把离心管置于13000rpm下离心5分钟，轻轻移动试管，用移液器将上清液体移至另一个尖底离心管中；
(4).重复步骤(2)(3)；
(5).向上述离心管中加入酚，再加入与酚等体积的氯仿或直接加入事先混好的1∶1的酚-氯仿混合物，盖紧管盖，反复颠倒或用DNA混合仪转动混匀，至两相混合均匀；
(6).用移液器将上清液移至另一个尖底试管中；
(7).向上述溶液中加入氯仿，盖紧管盖，反复颠倒或用DNA混合仪转动混匀，至两相混合均匀；
(8).将上清液转移至尖底试管中，加入-20℃的无水乙醇，颠倒混匀；
(9).把离心管置入高速离心机，以13000rpm离心8分钟，打开试管盖，将试管口朝下，轻轻地倒掉上清液；
(10).加入70％乙醇，清洗一次，把离心管放入高速离心机以13000rpm离心8分钟，打开试管盖，反转试管口朝下，轻轻倒掉上清液；
(11).将尖底管倒扣于吸水纸上，吸干残留液体后平卧，室温晾干；
(12).晾干后加入双蒸水，37℃保温或常温下过夜，使DNA溶解；
(13).将溶解后的DNA进行浓度测定，然后置于-20℃冰冻保存。
所述含有240ug/ml蛋白酶K的缓冲液为含有20mmol/L Tris.Cl(pH8.0)、200mmol/L EDTA(pH8.0)、1％SDS、240ug/ml蛋白酶K的水溶液。
本发明的提取方法省略了可致白细胞丢失的裂解红细胞过程，利用特制DNA提取溶液直接对全血成份进行消化处理，然后对消化液用酚、酚-氯仿混合液以及氯仿进行抽提，减少了白细胞的损失，大大提高了DNA的产量，基本做到无损失全量提取DNA。在有些情况下，DNA是一种极为宝贵的、不可再生的资源，尤其是那些已经去世的具有宝贵研究价值的遗传病患者，所留下的血液样本量极为有限，若采用常规的DNA的提取方法提取DNA，由于提取率低，会造成不可估量的损失，而采用本发明的方法则可取得较好的效果。本发明具有效果好、操作简单等优点。
具体实施方式
实施例：提取全血DNA，步骤如下：
(1).取冻融处理的ACD(柠檬酸、柠檬钠、葡萄糖)抗凝血100ul，置于1.5ml离心试管中，加入400ul含有240ug/ml蛋白酶K的缓冲液，振荡混匀，置于50℃水浴24小时，进行酶切，水浴过程中缓慢摇动离心管。
(2).酶切完成后，含有血液的混浊的溶液会变成清澈透明的红葡萄酒状，这时向离心管中加入等体积(约500ul)的pH为8.0重蒸饱和酚，盖紧管盖，反复颠倒20次左右或用DNA混合仪转动混匀10分钟，直至两相混合均匀，切忌使用振荡器或用力过猛。
(3).把离心管置于13000rpm下离心5分钟，轻轻移动试管，避免搅动界面，用1ml吸头的移液器将上清液体移至另一个1.5ml的尖底离心管中，为了最大限度回收DNA，可以稍稍吸带下层的酚。
(4).重复步骤(2)(3)。
(5).向上述离心管中加入250ul的酚，再加入250ul氯仿或直接加入500ul事先混好的1∶1的酚-氯仿混合物，盖紧管盖，反复颠倒20次左右或用DNA混合仪转动混匀10分钟，直至两相混合均匀。
(6).用1ml吸头的移液器将上清液移至另一个1.5ml的尖底试管中，不要吸入下层的酚。
(7).向上述溶液中加入500ul氯仿，盖紧管盖，反复颠倒20次左右或用DNA混合仪转动混匀10分钟，直至两相混合均匀。
(8).将上清液转移至1.5ml的尖底试管中，加入1ml-20℃的无水乙醇，颠倒混匀，这时会看到呈棉絮状析出的DNA。
(9).把离心管置入高速离心机，以13000rpm离心8分钟，打开试管盖，将试管口朝下，轻轻地倒掉上清液。
(10).加入1ml70％乙醇，清洗一次，把离心管放入高速离心机以13000rpm离心8分钟，打开试管盖，反转试管口朝下，轻轻倒掉上清液。
(11).将尖底管倒扣于吸水纸上，吸干残留液体后平卧，室温晾干10-15分钟。
(12).晾干后加入双蒸水(ddH2O)100-500ul，37℃保温3小时或常温下过夜，使DNA溶解。
(13).将溶解后的DNA进行浓度测定，然后置于-20℃冰冻保存。
所述含有240ug/ml蛋白酶K的缓冲液为含有20mmol/L Tris.Cl(pH8.0)、200mmol/LDTA(pH8.0)、1％SDS、240ug/ml蛋白酶K的水溶液。
采用传统酚～氯仿提取法提取全血DNA，与本发明方法提取相同血样DNA，1ml血得到DNA的量的比较：
应用独立样本T检验，两总体样本均为II型糖尿病病人血样，统计结果如下：
血样种类 样本量       1ml血得到DNA量的均数(μg) 标准差 标准误
  传统酚、氯仿法      293        34.15832      31.12173      1.81815
  本发明方法      96        246.36400      123.46601      12.60120
设置置信水平为α＝0.05，临界置信水平为0.000，远小于设置的置信水平，因此两种方法提取DNA平均1ml血得到DNA的量有显著性差异。由数据可知，用本发明的提取方法的提取率高，几乎可达100％。由表中数据可知，本发明的方法所提取的DNA与传统方法在OD260/280的比值方面略有下降，然而这并不影响所获得的DNA分子在常规的分子生物学操作的实验结果，因此这种变化是可以忽略的。
所用样本数据见下表1、2。
表1.传统酚、氯仿法提取糖尿病患者血样DNA
样品号 原液浓度   本试验提取DNA的量(μg)   本试验用血量(ml)   1ml全血提取DNA的量(μg) 260/280
  131730395055687480879197111112114126129134135137138141144147152157174   604.408147.15268.92253.52868.304101.23281.896103.8454.14458.60839.21689.784154.683.62458.71285.36151.872144.048156.26463.55259.384211.576144.104158.248146.48270.92260.312   60.440814.71526.89225.35286.830410.12328.189610.3845.41445.86083.92168.978415.468.36245.87128.53615.187214.404815.62646.35525.938421.157614.410415.824814.64827.09226.0312   0.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.5   120.881629.430413.78450.705613.660820.246416.379220.76810.828811.72167.843217.956830.9216.724811.742417.07230.374428.809631.252812.710411.876842.315228.820831.649629.29654.18452.0624   1.6271.2391.0931.5031.0671.5331.3611.0481.3251.4341.1481.4261.1141.4271.0590.9030.9891.2821.4911.3170.971.3581.2691.0821.2671.2131.22
  175   166.192   16.6192   0.5   33.2384   1.176
  1761771781801961971992012052152182212282322492502522532552572632672712852882922943063l1   67.088106.82477.93681.9289.032112.696125121.656106.968141.79276.691.16879.824106.65668.136216.464112.872163.01671.18456.768199.39276.27271.52850.02457.368187.26464.77665.232247.256   6.708810.68247.79368.1928.903211.269612.512.165610.696814.17927.669.11687.982410.66566.813621.646411.287216.30167.11845.676819.93927.62727.15285.00245.736818.72646.47766.523224.7256   0.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.5   13.417621.364815.587216.38417.806422.53922524.331221.393628.358415.3218.233615.964821.331213.627243.292822.574432.603214.236811.353639.878415.254414.305610.004811.473637.452812.955213.046449.4512   1.0621.3261.1381.0911.2961.1441.1591.0321.3020.9511.2191.0981.2821.3451.0851.5591.1611.3311.3611.0451.4691.2241.3411.1261.2421.6221.5461.3801.493
  312   107.816   10.7816   0.5   21.5632   1.177
  319322323330348350354357384402403408435442446448459471478489497505507511518519524528529   58.64852.464.728127.72241.072287.48118.31264.368346.78465.63265.60890.28882.304122.34484.85648.99256.776102.688110.032102.28850.14471.41653.168118.21677.264117.608357.71261.72105.368   5.86485.246.472812.77224.107228.74811.83126.436834.67846.56326.56089.02888.230412.23448.48564.89925.677610.268811.003210.22885.01447.14165.316811.82167.726411.760835.77126.17210.5368   0.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.5   11.729610.4812.945625.54448.214457.49623.662412.873669.356813.126413.121618.057616.460824.468816.97129.798411.355220.537622.006420.457610.028814.283210.633623.643215.452823.521671.542412.34421.0736   1.3551.3931.2211.0730.9210.9221.561.0331.6541.1121.8961.4511.3740.9171.2791.0071.2371.1611.1731.2521.1851.071.091.3261.1151.41.7431.9071.287
  531   144.672   14.4672   0.5   28.9344   1.119
  533534541564575580592609616621622628629634635655661678688693700751759762763764778787790   58.18469.35264.08176.952120.488231.784405.864193.5129.5203.57382343.5216.581174.56570.896180154462.474.572.592.550.555.5169258.587   5.81846.93526.40817.695212.048823.178440.586419.3512.9520.357.38.234.3521.658.117.456.57.08961815.446.247.457.259.255.055.5516.925.858.7   0.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.5   11.636813.870412.81635.390424.097646.356881.172838.725.940.714.616.468.743.316.234.91314.17923630.892.4814.914.518.510.111.133.851.717.4   1.1361.0921.1161.4791.5031.0921.1161.661.461.471.541.211.521.321.331.61.421.0881.391.261.6471.521.411.651.601.581.061.431.1
  792   93.5   9.35   0.5   18.7   1.13
  794797816817819820833834843851857861864865869871872873879881882887891892900902903915918   94298.5215181218213.579.5125.5468.808108.696220.8481131.5287.42442.8530.824139.5448.5713.560.5391.928254.5116.5118.5111.5106.95295479211.4   9.429.8521.518.121.821.357.9512.5546.880810.869622.0848113.1528.74244.2853.082413.9544.8571.356.0539.1928.25.4511.6511.8511.1510.69529.547.921.14   0.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.5   18.859.74336.243.642.715.925.193.761621.739244.1696226.357.48488.56106.164827.989.7142.712.178.38416.410.923.323.722.321.39041995.842.28   1.121.471.491.181.421.321.661.541.5621.6931.6131.771.5791.2921.951.211.631.781.191.5821.621.231.251.161.581.1421.341.71.441
  923   81.5   8.15   0.5   16.3   1.04
  924925930931943946947948949951958961967973978983984988990991101810201025102910331035103710431050   122109.5141241.5108.968598.5922559.55249.5239.559.568217340.5349.5175.585.536183174.5475212.5216.67297.562.553.598.5441.424116.5481.848578.296   12.210.9514.124.1510.896859.8592.255.95524.9523.955.956821.734.0534.9517.558.553618.317.4547.521.2521.66729.756.255.359.8544.142411.6548.184857.8296   0.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.5   24.421.928.248.321.7936119.7184.4111.91049.947.911.913643.468.169.935.117.107236.634.99542.543.334419.512.510.719.788.284823.396.3696115.6592   1.621.211.381.51.3241.721.531.4871.11.761.1491.571.771.771.71.1031.431.661.211.441.151.41.631.361.631.5881.591.2521.492
  1054   184.5   18.45   0.5   36.9   1.67
  10551057105810721084108510861092109610981101110211031106110811111114112311271129113311431147114811491161116511671174   61154.5146276.522270.27210398.5975659.68837.5766579.21638.1448099.5346.7664.584.5164.5127.5223118129.595.548178923   6.115.4514.627.6522.27.027210.39.859.75.65.96883.75766.57.92163.814489.9534.6766.458.4516.4512.7522.311.812.959.554.817.892.3   0.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.5   12.230.929.255.344.414.054420.619.719.411.211.93767.51521315.84327.62881619.969.35212.916.932.925.544.623.625.919.19.635.6184.6   1.111.181.21.751.3331.0521.31.541.51.461.0441.1251.071.3411.0461.771.631.721.541.581.781.761.61.171.21.741.621.271.21
  1175   834.5   83.45   0.5   166.9   1.17
  11861192120112021210121112321234123512361238124012481251125312581275127612801282128612941301130413071317131913261328   121.5107.5176.528638.7271.22461.608243.008427.304395.656304.624383.64321.60852.272304.592335.392124.128103.77654.456565.36879.92328.288157.3676.45656.6293.18479.64830.776186.152420.232   12.1510.7517.652863.8727.12246.160824.300842.730439.565630.462438.36432.16085.227230.459233.539212.412810.37765.445656.53687.99232.828815.7367.64565.6629.31847.96483.077618.615242.0232   0.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.5   24.321.535.3056127.74414.244812.321648.601685.460879.131260.924876.72864.321610.454460.918467.078424.825620.755210.8912113.073615.98465.657631.47215.291211.3258.636815.92966.155237.230484.0464   1.151.121.4531.3281.0391.091.2941.4371.4511.1481.5641.6761.0671.6551.561.6491.331.1521.5931.5671.5881.6531.7712.0511.2951.6311.1041.5791.672
  1333   58.296   5.8296   0.5   11.6592   1.282
  13361341134213451349135613571358136413651371137213911399140114021405140914181421142214261429143014381442144514471448   123.256152.04494.576404.08306.648156.9651.616153.48210.832136.104107.04116.272227.624127.32157.24858.968254.784141.896253.368213.832211.46475.48173.15286.528178.60892.288195.84122.408138.92   12.325615.20449.457640.40830.664815.6965.161615.34821.083213.610410.70411.627222.762412.73215.72485.896825.478414.189625.336821.383221.14647.54817.31528.652817.86089.228819.58412.240813.892   0.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.5   24.651230.40898.915280.81661.329631.39210.323230.69642.166427.220821.40823.254445.524825.46431.449611.793650.956828.379250.673642.766442.292815.09634.630417.305635.721618.457639.16824.481627.784   1.5581.2771.5911.6781.7031.3381.5511.4061.5451.411.5381.5841.5091.321.3381.1331.381.5281.6781.2541.5581.4491.481.5141.1931.2881.4961.861.582
  1451   122.368   12.2368   0.5   24.4736   2.101
  1453146914711473147414751481148214841488148914921495149615031504150715141517153015341536154215461547   66.35277.03274.968240.10437.3684.144128.888132.15295.45691.61681.712261.784132.26456.544300.439.25654.12898.75273.704341.184108106.55249.27288.68866.208   6.63527.70327.496824.01043.7368.414412.888813.21529.54569.16168.171226.178413.22645.654430.043.92565.41289.87527.370434.118410.810.65524.92728.86886.6208   0.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.50.5   13.270415.406414.993648.02087.47216.828825.777626.430419.091218.323216.342452.356826.452811.308860.087.851210.825619.750414.740868.236821.621.31049.854417.737613.2416   1.6951.4531.3611.4521.1851.2721.4561.5011.5611.5461.3151.21.3031.4461.1471.1321.4321.3971.2841.221.3761.521.2121.0561.372
表4.本发明方法提取糖尿病患者血样DNA
样品号 原液浓度   本试验提取DNA的量(μg)   本试验用血量(ml)   1ml全血提取DNA的量(μg) 260/280
  310162048678285939599101105106165167183186205211225234235237238239240   427.592443.368396.944171.16154.216154.712152.312133.824184.384163.504187.672206.72182.512208.84289.584157.824202.072245.01698.928258.072477.552212.704237.94458.76212.344114.376145.704   42.759244.336839.694417.11615.421615.471215.231213.382418.438416.350418.767220.67218.251220.88428.958415.782420.207224.50169.892825.807247.755221.270423.79445.87621.234411.437614.5704   0.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.1   427.592443.368396.944171.16154.216154.712152.312133.824184.384163.504187.672206.72182.512208.84289.584157.824202.072245.01698.928258.072477.552212.704237.94458.76212.344114.376145.704   1.0151.0291.0251.0041.0451.0221.0221.0371.0451.0431.0611.2151.0761.0811.0541.0561.0331.0451.0791.5561.0621.2171.0691.1721.2861.0451.042
  248   179   17.9   0.1   179   1.078
  277281282283284290291296300301302303304305307308309316318320326328329331344345365367369   307.472111.104234.664271.56181.688430.304212.872249.144466.776405.688255.752334.216149.088110.592200.352162.64178.928500.008182.496200.56205.432116.528178.4303.92292.856298.60885.76151.232147.84   30.747211.110423.466427.15618.168843.030421.287224.914446.677640.568825.575233.421614.908811.059220.035216.26417.892850.000818.249620.05620.543211.652817.8430.39229.285629.86088.57615.123214.784   0.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.1   307.472111.104234.664271.56181.688430.304212.872249.144466.776405.688255.752334.216149.088110.592200.352162.64178.928500.008182.496200.56205.432116.528178.4303.92292.856298.60885.76151.232147.84   1.0241.1081.0321.0271.1961.4331.0381.0861.4871.2441.0261.0341.0861.0611.0251.0341.0841.1081.0951.0251.0471.0191.1031.0420.9941.1511.291.0391.077
370 190.136 19.0136 0.1 190.136 1.032
  371372373376377378379380382383385386389390391394395396398399404421422423424428498500501   197.352338.712420.344655.408330.88266.552280.03277.504127.824499.496513.2486.536450.56451.992157.16175.128162.208273.64125.544155.776215.848201.99280.176242.8232.04173.88599.704321.376200.68   19.735233.871242.034465.540833.08826.655228.00327.750412.782449.949651.3248.653645.05645.199215.71617.512816.220827.36412.554415.577621.584820.19928.017624.2823.20417.38859.970432.137620.068   0.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.10.1   197.352338.712420.344655.408330.88266.552280.03277.504127.824499.496513.2486.536450.56451.992157.16175.128162.208273.64125.544155.776215.848201.99280.176242.8232.04173.88599.704321.376200.68   1.0251.0641.0581.0471.0321.0251.0411.2291.2091.1091.1141.0691.1861.1741.0531.0451.1121.3641.1281.0311.0871.1141.1021.1780.9781.0751.0471.0081.005
  522   243.04   24.304   0.1   243.04   1.095
  525527530532535536539545   214.264214.24183.168104.16370.92179.264276.264277.024   21.426421.42418.316810.41637.09217.926427.626427.7024   0.10.10.10.10.10.10.10.1   214.264214.24183.168104.16370.92179.264276.264277.024   1.0761.3251.0530.9921.010.9861.0070.971

Claims (2)

1.一种全血DNA的提取方法，其特征在于：包括以下步骤：
(1).取冻融处理的ACD抗凝血，置于离心试管中，加入含有240ug/ml蛋白酶K的缓冲液，振荡混匀，置于50℃水浴20～28小时，水浴过程中缓慢摇动离心管；
(2).酶切完成后，向离心管中加入等体积的pH为8.0的重蒸饱和酚，盖紧管盖，反复颠倒或用DNA混合仪转动混匀，直至两相混合均匀；
(3).把离心管置于13000rpm下离心5分钟，轻轻移动试管，用移液器将上清液体移至另一个尖底离心管中；
(4).重复步骤(2)(3)；
(5).向上述离心管中加入酚，再加入与酚等体积的氯仿或直接加入事先混好的1∶1的酚-氯仿混合物，盖紧管盖，反复颠倒或用DNA混合仪转动混匀，至两相混合均匀；
(6).用移液器将上清液移至另一个尖底试管中；
(7).向上述溶液中加入氯仿，盖紧管盖，反复颠倒或用DNA混合仪转动混匀，至两相混合均匀；
(8).将上清液转移至尖底试管中，加入-20℃的无水乙醇，颠倒混匀；
(9).把离心管置入高速离心机，以13000rpm离心8分钟，打开试管盖，将试管口朝下，轻轻地倒掉上清液；
(10).加入70％乙醇，清洗一次，把离心管放入高速离心机以13000rpm离心8分钟，打开试管盖，反转试管口朝下，轻轻倒掉上清液；
(11).将尖底管倒扣于吸水纸上，吸干残留液体后平卧，室温晾干；
(12).晾干后加入双蒸水，37℃保温或常温下过夜，使DNA溶解；
(13).将溶解后的DNA进行浓度测定，然后置于-20℃冰冻保存。
2.根据权利要求1所述的一种全血DNA的提取方法，其特征在于：所述缓冲液为含有20mmol/LTris.Cl、200mmol/L EDTA、1％SDS、240ug/ml蛋白酶K的水溶液。
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104017800A (zh) * 2014-06-20 2014-09-03 益百尚(北京)生物技术有限责任公司 一种用于血液中全基因组dna提取试剂盒及其方法
CN104152438A (zh) * 2014-08-15 2014-11-19 四川农业大学 用于提取家禽dna的细胞裂解液、试剂盒和方法
CN106754869A (zh) * 2016-11-30 2017-05-31 成都大学 一种血液基因组dna的提取方法

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104017800A (zh) * 2014-06-20 2014-09-03 益百尚(北京)生物技术有限责任公司 一种用于血液中全基因组dna提取试剂盒及其方法
CN104152438A (zh) * 2014-08-15 2014-11-19 四川农业大学 用于提取家禽dna的细胞裂解液、试剂盒和方法
CN106754869A (zh) * 2016-11-30 2017-05-31 成都大学 一种血液基因组dna的提取方法

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