CN101210227A - Fermentation technique for gaining high active bacillusmusilaginosis in biofertilizer - Google Patents
Fermentation technique for gaining high active bacillusmusilaginosis in biofertilizer Download PDFInfo
- Publication number
- CN101210227A CN101210227A CNA2006101483353A CN200610148335A CN101210227A CN 101210227 A CN101210227 A CN 101210227A CN A2006101483353 A CNA2006101483353 A CN A2006101483353A CN 200610148335 A CN200610148335 A CN 200610148335A CN 101210227 A CN101210227 A CN 101210227A
- Authority
- CN
- China
- Prior art keywords
- zymotechnique
- fermentation
- bacillusmusilaginosiengineering
- powder
- cooling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a fermentation process for acquiring high-activity bacillus mucilaginosus from bio-fertilizer. The raw materials adopted in the invention comprises, by weight ratio, fermenting seed liquid of the bacillus mucilaginosus 0.4% to 0.8%, corn flour 1.5% to 4%, beau powder 1.0% to 2.0%, wheat middling 0.5% to 1.2%, fish meal 0.2% to 0.8%, calcium carbonate 0.05% to 0.15%, inorganic salt 0.01% to 0.08% and water 92% to 96%. The fermentation process comprises directly feeding materials, fast disinfecting and cooling single-layer fermentation tank, improving the fermentation temperature, reducing the fermentation tank pressure and the ventilation ratio, etc, thus reducing the production cost, shortening the fermentation cycle and acquiring the high-activity bacillus mucilaginosus. The process is suitable for production of bio-fertilizer.
Description
Technical field
The present invention relates to the mushroom zymotechnique, relate to a kind of zymotechnique that obtains high reactivity bacillusmusilaginosiengineering in the bio-feritlizer specifically.
Background technology
Potassium is the needed macroelement of plant growth, soil and fertilising are the main sources of crop potassium nutrition, every mu of about 1740Kg of soil potassium content, and wherein have only 2% replacement potassium and water-soluble potassium to be absorbed by crop, and all the other mineral potassiums can't directly be absorbed by crop.Bacillusmusilaginosiengineering has very strong capacity of decomposition to mineral potassium, also has the effect of fixed nitrogen, phosphorus decomposing simultaneously, so bacillusmusilaginosiengineering is used widely in the production of bio-feritlizer.
Zymotechnique commonly used at present is:
Adopt raw material (in weight ratio):
Bacillusmusilaginosiengineering fermentation seed liquid 0.5～1%
Starch 1～3%
Yeast powder 0.5～0.7%
Peptone 0.5～0.8%
Sodium-chlor 0.2～0.3%
Potassium hydrogen phosphate 0.1～0.2%
Ammonium sulfate 0.15～0.20%
Ammonium molybdate 0.01～0.08%
Water 96.54～97.00%
Zymotechnique is:
1. feed intake: earlier the raw material of preparation is according to the above ratio put into a mixer, stir evenly, and adjusting pH value is 7.5, pump into fermentor tank with underflow pump again.
2, sterilization: traditional method, enter steam with the fermentor tank interlayer earlier, fermented feed liquid is preheated 90 ℃, directly advance steam subsequently in fermentor tank, make batch can be warmed up to 121 ℃, tank pressure is raised to 0.11Mpa, keeps 30 minutes.
3, cooling: after sterilization finishes, make jar interior sterile air that feeds, when making feed temperature naturally cool to 100 ℃, enter water coolant at interlayer and cool off.
4, seed fermentation: with 0.5% ferment-seeded is that bacillusmusilaginosiengineering is joined 99.5% water and got involved in the seeding tank and ferment, and the seed culture time is 8～10 hours.
5, inoculation: 1% of the seed liquor that ferments is inserted fermentor tank.
6, fermentation culture: postvaccinal seed and fermentor tank were ventilating ratio 1: 0.5, and tank pressure 1.0Mpa cultivates under the condition that temperature is 30 ± 2 ℃, and the fermentor cultivation time is 16～20 hours, and fermentation ends fermentor tank viable bacteria plate count is 10～1,500,000,000/ml.
The shortcoming of this zymotechnique:
1, fermentation raw material generally adopts chemical pure or food grade materials, the cost height, and use in the general laboratory that adapts to of prescription, and solid concentration is low.
2, feed intake and use mixer premix, troublesome poeration.
By the interlayer preheating, disinfecting time is long when 3, sterilizing, waste steam, consumes energy.
4, when cooling elder generation naturally cooling has prolonged temperature fall time, makes the feed liquid length of holding time when high temperature, its nutrient loss, component generation chemical transformation.
5, fermentation period is longer, and fermentation activity is low.
The present inventor provides a kind of zymotechnique that obtains the high reactivity bacillusmusilaginosiengineering, thereby has shortened fermentation period by improving fermentation raw material prescription and zymotechnique, has reduced cost, can be used for the production of bio-feritlizer better.
Summary of the invention
The object of the present invention is to provide a kind of zymotechnique that obtains high reactivity bacillusmusilaginosiengineering in the bio-feritlizer.
Purpose of the present invention realizes by following measure:
The raw material that zymotechnique of the present invention adopts comprises in weight ratio: bacillusmusilaginosiengineering fermentation seed liquid 0.4～0.8%, Semen Maydis powder 1.5～4%, bean cake powder 1.0～2.0%, wheat time powder 0.5～1.2%, fish meal 0.2～0.8%, lime carbonate 0.05～0.15%, inorganic salt 0.01～0.08% and water 92～96%
Described zymotechnique may further comprise the steps: feed intake, sterilize, cooling, seed fermentation, inoculation and cultivation.
Wherein said bacillusmusilaginosiengineering is single or mixes.
Wherein said inorganic salt are single or mix that described inorganic salt are selected from sal epsom, sodium-chlor, potassium hydrogen phosphate, sodium hydrogen phosphate and calcium chloride.
The wherein said employing that feeds intake directly feeds intake, and direct air inlet method is adopted in described sterilization, and direct interlayer water inlet cooling is adopted in described cooling.
In culturing step, ventilating ratio is 1: 1～1: 4, and tank pressure is 0.5～0.9Mpa, and temperature is 37 ± 2 ℃, and incubation time is 8～10 hours.
Carbon source adopts starch in the composition of raw materials of tradition bacillusmusilaginosiengineering zymotechnique, nitrogenous source adopts yeast powder and peptone, the price of these raw materials is all somewhat expensive, and carbon source adopts Semen Maydis powder in the zymotechnique of the present invention, nitrogenous source adopts bean cake powder and fish meal, these all are agricultural byproducts, and price descends 80～90% than original, has reduced production cost.Materials of wheat of the present invention in addition time powder is to be that raw material grinds the byproduct that obtains behind the various flour with the wheat, contains VITAMIN, somatomedin and zymin, can promote the bacillusmusilaginosiengineering fermentation.
Adopt when the present invention feeds intake directly to feed intake, made things convenient for production operation; When cooling sterilization adopted the cooling of sterilizing fast of individual layer fermentor tank, can reduce the use of quantity of steam, and save time; Improved leavening temperature during fermentation, reduced fermentation tank pressure and ventilating ratio, shortened fermentation period like this, improved the fermentation unit activity, the zymophyte number reaches 45～5,000,000,000/ml, and the gemma rate of formation reaches more than 90%.
Adopt the high reactivity bacillusmusilaginosiengineering of zymotechnique gained of the present invention can be widely used in the production of bio-feritlizer, further improved the fertilizer efficiency of bio-feritlizer.
Embodiment
Below for a more detailed description with embodiment to the present invention.These embodiment only are the descriptions to best mode for carrying out the invention, scope of the present invention are not had any restriction.
Embodiment 1:
Obtain the proportioning raw materials following (in weight ratio) of the zymotechnique of high reactivity bacillusmusilaginosiengineering:
Bacillusmusilaginosiengineering fermentation seed liquid 0.5%
Semen Maydis powder 3.8%
Bean cake powder 1.2%
Wheat time powder 0.5%
Fish meal 0.2%
Lime carbonate 0.1%
Sodium-chlor 0.05%
Water 93.65%
Zymotechnique:
Directly add the water constant volume in fermentor tank, bubbling air directly drops into jar interior a stirring with above-mentioned raw materials by proportioning in advance, with direct air inlet method sterilization, will expect in the sterilization that temperature directly is raised to 121 ℃, tank pressure maintains 0.11Mpa, kept 30 minutes, and directly cooled off after sterilization finishes by the interlayer water inlet.Be that bacillusmusilaginosiengineering is joined 99.5% water and inserted in the seeding tank and ferment simultaneously with 0.5% ferment-seeded, the seed culture time is 5 hours.Then 1% of the seed liquor that ferments is inserted fermentor tank, ventilating ratio 1: 1, tank pressure 0.5Mpa, the condition bottom fermentation that temperature is 37 ± 2 ℃ was cultivated 8 hours, fermentation ends.
Embodiment 2:
Obtain the proportioning raw materials following (in weight ratio) of the zymotechnique of high reactivity bacillusmusilaginosiengineering:
Bacillusmusilaginosiengineering fermentation seed liquid 0.4%
Semen Maydis powder 2.1%
Bean cake powder 1.8%
Wheat time powder 0.8%
Fish meal 0.6%
Lime carbonate 0.08%
Sal epsom 0.03%
Water 94.19%
Zymotechnique is with embodiment 1, and wherein ventilating ratio is 1: 2, tank pressure 0.6Mpa.
Embodiment 3:
Obtain the proportioning raw materials following (in weight ratio) of the zymotechnique of high reactivity bacillusmusilaginosiengineering:
Bacillusmusilaginosiengineering fermentation seed liquid 0.8%
Semen Maydis powder 3.5%
Bean cake powder 1.3%
Wheat time powder 1.0%
Fish meal 0.5%
Lime carbonate 0.12%
Sodium hydrogen phosphate 0.03%
Calcium chloride 0.04%
Water 92.71%
Zymotechnique is with embodiment 1, and wherein ventilating ratio is 1: 3, and tank pressure 0.9Mpa, incubation time are 10 hours.
The bacillusmusilaginosiengineering that adopts zymotechnique of the present invention to obtain, its zymophyte number reaches 45～5,000,000,000/ml, and the gemma rate of formation reaches more than 90%.
Claims (8)
1. zymotechnique that obtains high reactivity bacillusmusilaginosiengineering in the bio-feritlizer, it is characterized in that the raw material that described zymotechnique adopts comprises in weight ratio: bacillusmusilaginosiengineering fermentation seed liquid 0.4～0.8%, Semen Maydis powder 1.5～4%, bean cake powder 1.0～2.0%, wheat time powder 0.5～1.2%, fish meal 0.2～0.8%, lime carbonate 0.05～0.15%, inorganic salt 0.01～0.08% and water 92～96%;
Described zymotechnique may further comprise the steps: feed intake, sterilize, cooling, seed fermentation, inoculation and cultivation.
2. zymotechnique according to claim 1, wherein said bacillusmusilaginosiengineering are single or mix.
3. zymotechnique according to claim 1, wherein said inorganic salt are single or mix.
4. according to claim 1 or 3 described zymotechniques, wherein said inorganic salt are selected from sal epsom, sodium-chlor, potassium hydrogen phosphate, sodium hydrogen phosphate and calcium chloride.
5. zymotechnique according to claim 1, the wherein said employing that feeds intake directly feeds intake.
6. zymotechnique according to claim 1, wherein said sterilization adopt direct air inlet method.
7. zymotechnique according to claim 1, wherein said cooling adopt direct interlayer water inlet cooling.
8. zymotechnique according to claim 1, in the wherein said culturing step, ventilating ratio is 1: 1～1: 4, and tank pressure is 0.5～0.9Mpa, and temperature is 37 ± 2 ℃, and incubation time is 8～10 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006101483353A CN101210227A (en) | 2006-12-29 | 2006-12-29 | Fermentation technique for gaining high active bacillusmusilaginosis in biofertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006101483353A CN101210227A (en) | 2006-12-29 | 2006-12-29 | Fermentation technique for gaining high active bacillusmusilaginosis in biofertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101210227A true CN101210227A (en) | 2008-07-02 |
Family
ID=39610483
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2006101483353A Pending CN101210227A (en) | 2006-12-29 | 2006-12-29 | Fermentation technique for gaining high active bacillusmusilaginosis in biofertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101210227A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102747001A (en) * | 2011-07-05 | 2012-10-24 | 天津理工大学 | Physical pretreatment fermentation process for corn straws by using flora degradation |
| CN104480035A (en) * | 2014-11-14 | 2015-04-01 | 武汉科诺生物科技股份有限公司 | Paenibacillus mucilaginosus high producing strain, and culturing method and use thereof |
-
2006
- 2006-12-29 CN CNA2006101483353A patent/CN101210227A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102747001A (en) * | 2011-07-05 | 2012-10-24 | 天津理工大学 | Physical pretreatment fermentation process for corn straws by using flora degradation |
| CN104480035A (en) * | 2014-11-14 | 2015-04-01 | 武汉科诺生物科技股份有限公司 | Paenibacillus mucilaginosus high producing strain, and culturing method and use thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1030761C (en) | Compound organic fertilizer containing multiple bacterial spawns and preparation thereof | |
| CN107162656A (en) | A kind of feces of livestock and poultry superhigh temperature compost method | |
| CN104529642A (en) | Organic, inorganic and microbe compound fertilizer for rice production and preparation method thereof | |
| CN102276367B (en) | Biological-organic-inorganic compound fertilizer and preparation method thereof | |
| CN108218565A (en) | Alkaline soil improver and preparation method thereof | |
| CN101905985B (en) | Method for preparing microorganism-decomposing agent used for producing biological organic fertilizer by livestock and poultry manure fermentation | |
| CN102951966A (en) | Production method for compound microbial manure | |
| CN102599341B (en) | Fermentation method of soybean meal | |
| CN103342597A (en) | Preparation method of special compound microbial fertilizer for wheat for improving soil and product thereof | |
| CN101709272A (en) | Method for preparing feed yeast | |
| CN101214006B (en) | Method for using EM original liquid fermentation apple pomace to produce protein feed | |
| CN1254696A (en) | Compound biological fertilizer | |
| CN112645746A (en) | Animal manure straw bio-organic fertilizer and preparation method thereof | |
| CN106542926A (en) | A kind of Se-enriched microbial organic fertilizer with humic acid membrane wrapping and preparation method thereof | |
| CN110384178B (en) | Lactic acid bacteria culture prepared based on vinasse and application of lactic acid bacteria culture in animal feed | |
| CN104177138A (en) | Biological bacterial fertilizer prepared through solid fermentation technology and application of biological bacterial fertilizer | |
| CN101659570A (en) | Preparation method of biofertilizer | |
| CN101885625A (en) | Solid fermentation method for producing microbial agent by using biomass power plant ash as carrier | |
| CN102219575B (en) | Industrial method for producing bio-fertilizer inoculant by utilizing decayed cow dung as raw material | |
| CN1240828C (en) | Fermenting liquid for feed with microbiological colonies | |
| CN108147868A (en) | A kind of method, bamboo fermentate and its application of the decomposed fermentation of bamboo | |
| CN102010239B (en) | Organic fertilizer and preparation method thereof | |
| CN1286776C (en) | Composite microbial manure and method for production thereof | |
| CN106748042A (en) | Biological organic fertilizer leavening and preparation method thereof | |
| CN101210227A (en) | Fermentation technique for gaining high active bacillusmusilaginosis in biofertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20080702 |