CN101199292A - Biological potentiating agent for increasing preventing and controlling effect of living insecticide, composite, preparation method and application - Google Patents

Biological potentiating agent for increasing preventing and controlling effect of living insecticide, composite, preparation method and application Download PDF

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CN101199292A
CN101199292A CNA2007100664553A CN200710066455A CN101199292A CN 101199292 A CN101199292 A CN 101199292A CN A2007100664553 A CNA2007100664553 A CN A2007100664553A CN 200710066455 A CN200710066455 A CN 200710066455A CN 101199292 A CN101199292 A CN 101199292A
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biological
potentiating agent
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composition
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杨佩文
尚慧
董丽英
刘树芳
池亨镇
李家瑞
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Institute of Agricultural Environment and Resources of Yunnan Academy of Agricultural Sciences
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Institute of Agricultural Environment and Resources of Yunnan Academy of Agricultural Sciences
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    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract

The invention provides a biological synergist and the composition for improving the control efficiency of biotic insecticide, as well as the preparation method and the application. The biological synergist of the invention is composed of 15-30g of fresh hen egg yolk, 45-90ml of edible vegetable oil and clear water as a residue, and the bulk volume is 700-1500ml. The 2 percent of avermectin emulsifiable solution containing 3 percent-8 percent of biological synergist has the pest average rate of decrease of 85.79 percent-90.93 percent seven days after the application of synergist, and has the increased average control efficiency by 5.85 percent-12.19 percent compared with only using the 2 percent of avermectin emulsifiable solution seven days after the application of synergist. The 10 billion of living spores/grams of Bacillus thuringiensis wettable powder (BT) containing 3 percent-8 percent of biological synergist has the pest average rate of decrease of 83.42 percent-86.69 percent seven days after the application of synergist, and has the increased average control efficiency by 19.48 percent-24.16 percent compared with only using the 10 billion living spores/grams of Bacillus thuringiensis wettable powder (BT) seven days after the application of synergist. The biological synergist and the composition will not pollute the environment and is safe to human beings and livestock, and the preparing process is simple and the price is low. The invention can obviously improve the control efficiency of biotic insecticide and has huge application potential.

Description

Improve biological potentiating agent and composition, preparation method and the application of biological insecticides control efficiency
Technical field the invention belongs to biological pesticide technical field.The synergist that relates to biopesticide is specifically related to a kind of composition, preparation method and application that improves the biological potentiating agent of biological insecticides control efficiency and contain this biological potentiating agent.
In the later stage sixties 20th century of background technology, the pesticide hazards problem is on the rise, and countries in the world are progressively developed the developing direction of biopesticide as novel pesticide to development low toxicity, efficient novel pesticide.Advantages such as that biopesticide has is nontoxic, free from environmental pollution to the person poultry safety, noresidue, non-killed natural enemies and beneficial organism are fit to the requirement of nuisanceless production, are the directions of agricultural chemicals development from now on.Along with the countries in the world consumer increases day by day to the demand of non-polluted farm product, the development of biopesticide has broad prospects.According to estimates, will reach 8,000,000,000 dollars, account for half of agricultural chemicals sales volume to whole world biopesticide sales volume in 2008.The biopesticide active ingredient kind that China's year in 2002 was registered is 85,1,800,000,000 yuan of annual value of production, and on average each kind annual value of production is more than 200 ten thousand yuan, and China has become maximum in the world jinggangmeisu, Avermectin, gibberellin producing country.
In recent years, though biopesticide has obtained comprehensive exploitation and used widely,, hamper the development of biopesticide because the characteristic of itself also exists some distinct issues and difficulty at aspects such as research, exploitation, production and application.The biopesticide drug effect is slow, the term of validity short, and the insecticidal and antibacterial spectrum is narrow relatively, in a short time to a little less than the Pest management power, can not reduce the disease index of insect insect density and disease rapidly, is difficult to tackle sudden and crushing damage by disease and insect.In addition, the stability of medicament also is a key factor of its development of restriction, particularly is the product of active ingredient with the living microorganism, and biologically active descends fast, and the warranty period is short, is subject to the influence of external environment again, causes the control efficiency instability.Therefore the effectiveness that improves biopesticide becomes important subject.
Summary of the invention the purpose of this invention is to provide a kind of bio-synergistic preparation that is used to improve the biological insecticides control efficiency.This bio-synergistic preparation is the mixed liquor that contains edible vegetable oil and fresh egg yolk.This bio-synergistic preparation is free from environmental pollution, and to people, animal safety, and preparation technology is simple, cheap.
The present invention also provides the method for preparing above-mentioned biological potentiating agent, the composition that contains above-mentioned biological potentiating agent and their application.
Technical scheme of the present invention is:
One, improve the biological potentiating agent of biological insecticides control efficiency, it is characterized in that: form by following content and component:
Fresh egg yolk 15-30g, edible vegetable oil 45-90mL, the cumulative volume that closes of these components of clear water surplus mL is 700-1500mL.
The content of the preferred biological potentiating agent that the present invention recommends and component are formed: fresh egg yolk 20g, and edible vegetable oil 60mL, clear water are surplus mL, the cumulative volume that closes of these components is 1000mL.
Above-mentioned fresh egg yolk is meant fresh raw egg yolk.
Two, the composition that contains above-mentioned biological potentiating agent, it is characterized in that: said composition is mixed by biological insecticides and biological potentiating agent of the present invention and is formed, and its described biological potentiating agent consumption is the 3%-8% (volume ratio) of the biological insecticides solution usage prepared.
The preferred composition that contains above-mentioned biological potentiating agent that the present invention recommends is characterized in that: said composition is mixed by 2% abamectin emulsifiable concentrate and biological potentiating agent of the present invention and is formed; Its described biological potentiating agent consumption is the 3%-8% of the 2% abamectin emulsifiable concentrate solution usage volume ratio prepared.
Another of the present invention's recommendation contains the preferred composition of above-mentioned biological potentiating agent, it is characterized in that: said composition is mixed by 10,000,000,000 living spores/gram bacillus thuringiensis,Bt wetting powder (hereinafter to be referred as Bt) and above-mentioned biological potentiating agent and is formed; Its described biological potentiating agent consumption is 5% of the Bt solution usage volume ratio prepared.
The composition that below respectively contains biological potentiating agent, another consumption of recommending contained biological potentiating agent are 5% of the biological insecticides solution usage volume ratio prepared.
Three, the preparation method of biological potentiating agent of the present invention is characterized in that: this method may further comprise the steps:
(a) take out the clear water rinsing of fresh yolk, get yolk 15-30g;
(b) yolk and proper amount of clear water are stirred, add edible vegetable oil 45-90mL again, stir, add clear water at last to 700-1500mL, and mix, not stratified in clear water until the preparation of making, can fully be dissolved into the emulsion of milk shape, place cold compartment of refrigerator (refrigerator cold-storage typical temperature) standby.
More than be formulated in normal temperature, normal pressure carries out.
Four, contain the application of the composition of biological potentiating agent of the present invention, it is characterized in that: carry out spray application at insect pest initial stage of origination or Sheng phase.
Five, the control that contains the diamond-back moth insect of composition on the cauliflower crop of biological potentiating agent of the present invention is used.
Methods such as the compound method of the Biocidal agent solution of preparing of the present invention, field rotating medicine amount are the method that routine or specification are introduced.
Edible vegetable oil of the present invention is commercially available vegetable oil.Be used for edible vegetable oil of the present invention and fresh egg yolk source and be not particularly limited, those skilled in the art can select suitable edible vegetable oil and fresh egg yolk.
Employing contains the biological insecticides of bio-synergistic preparation of the present invention, and it is obvious to improve the biological insecticides control efficiency.2% abamectin emulsifiable concentrate that contains 3%-8% bio-synergistic preparation of the present invention, after the dispenser 7 days, the insect average rate of decrease was 85.79%-90.93%, the average correction control efficiency is 86.90%-91.59%.After the dispenser 7 days is 5.85%-12.19% than single average control efficiency raising rate with 2% abamectin emulsifiable concentrate, and average correction control efficiency raising rate is 5.26%-10.94%.
The Bt that contains 3%-8% bio-synergistic preparation of the present invention, after the dispenser 7 days, the insect average rate of decrease was 83.42%-86.69%, the average correction control efficiency is 84.69%-87.80%.After the dispenser 7 days is 19.48%-24.16% than single average control efficiency raising rate with Bt, and average correction control efficiency raising rate is 17.41%-21.72%.
Biological potentiating agent of the present invention has the advantage that significantly improves the biological insecticides control efficiency, and is very big as the auxiliary agent application potential of biological insecticides.
Below in conjunction with concrete implementation column beneficial effect of the present invention is described.
Embodiment
Embodiment 1:
One, experimental condition
1, subjects, crop and breed selection: subjects is diamond-back moth [Plutella xylostella (Linnaeus)], and trial crops is a cauliflower, and kind is for too white.
2, environmental condition: the experimental field is positioned at Yang Guang town, Tonghai County, Yunnan Province peasant household vegetables field, and soil is sandy paddy soil, alkalescent, middle fertility.Irrigation conditions is better, and management is undertaken by the locality custom, 1 water of every 3-5 days border irrigation, and duration of test does not have applying fertilizer.Sowing on August 9th, 2007, September 20 transplanted, 45000 strains/hm2, the medium generation of diamond-back moth.The cultivation condition uniformity of all experimental plots, the Pass Test requirement.
Two, experimental scheme and arrangement
1, test medicine:
(1) test is formed by following content and component with biological potentiating agent of the present invention:
Fresh egg yolk 20g, edible vegetable oil 60mL, the clear water surplus, cumulative volume is 1000mL.
Its compound method is by the preparation method of biological potentiating agent of the present invention " three, " preparation in the technical scheme of the present invention.
(2) 2% abamectin emulsifiable concentrates, the Huizhou City Zhongxun Chemical Industry Co., Ltd produces, and is mixed with liquid by the requirement of medication instruction book, 187.50 milliliters/hectare of dispenser dosage (amount of formulation);
(3) Bt, Shandong Dong Tainongization chemical industry Co., Ltd produces, and is mixed with liquid by the requirement of medication instruction book, dispenser dosage (amount of formulation) 2250.00 gram/hectares.
Volume ratio 3%, 5%, 8% consumption that biological potentiating agent of the present invention is pressed the Biocidal agent solution of being prepared respectively adds, and mixes.
2, sub-district area and repetition: small plot experiment adopts randomized arrangement.8 processing are established in test, 1 processing of blank, totally 9 processing, 4 repetitions, 36 sub-districts, every sub-district 20m 2, the sub-district area is 720m altogether 2, establish guard row.
3, application method: adopt the spraying of WS-16 type (Shandong Wish Plant Protection Machinery Co., Ltd.) knapsack hand sprayer.To all even and fine spraying of complete stool (leave dual sides).Spray date is on October 6th, 2007, and 6 leaf phases of cauliflower, diamond-back moth low instar larvae are contained the phase of sending out, spray medicine 1 time.Duration of test does not have other damage by disease and insect of control.
4, meteorology and soil information: 1 day no rain behind the dispenser same day and the medicine, light rain was arranged in the 2nd day behind the medicine, there was not rain behind the medicine in 3-7 days.19 ℃ of duration of test mean temperature of air, 26 ℃ of maximum temperatures, 12 ℃ of minimum temperatures (weather is fine during dispenser, 18.7 ℃ of daily mean temperatures, relative moisture 65%).There is not other adverse weather condition that influences result of the test.Experimental field physical features is smooth, and soil fertility is higher, and duration of test waters once, and the field does not have weeds.
Three, investigation method, time and number of times
5 samplings in every sub-district, every some direct motion fixed point investigation 5 strains, and the generation radix of record diamond-back moth.Later on, investigate the residual borer population of each strain, calculate insect population go down rate and preventive effect respectively at the 1st day behind the medicine, the 3rd day and the 7th day.Adopt the DMRT method to carry out the significance of difference test investigation result.
Figure S2007100664553D00041
Figure S2007100664553D00051
Four, result and analysis
Table 1 result of the test and table 3 show: when 2% abamectin emulsifiable concentrate is 187.50 milliliters/hectare in the administered formulation amount, single agent control diamond-back moth, after the dispenser 1 day-7 days, average rate of decrease was 65.93%-81.05%, and the average correction control efficiency is 65.98%-82.56%.
Adopting 2% abamectin emulsifiable concentrate to add biological potentiating agent control diamond-back moth of the present invention, is 3% o'clock containing described biological potentiating agent amount of application, and after the dispenser 1 day-7 days, average rate of decrease was 69.07%-85.79%, and the average correction control efficiency is 69.13%-86.90%.Containing described biological potentiating agent amount of application is 5% o'clock, and after the dispenser 1 day-7 days, average rate of decrease was 76.53%-90.49%, and the average correction control efficiency is 76.61%-91.12%.Containing described biological potentiating agent amount of application is 8% o'clock, and after the dispenser 1 day-7 days, average rate of decrease was 76.47%-90.93%, and the average correction control efficiency is 76.54%-91.59%.
Table 2 result of the test and table 4 show: when Bt is 2250.50 gram/hectares in the administered formulation amount, and single agent control diamond-back moth, after the dispenser 1 day-7 days, average rate of decrease was 52.19%-69.82%, the average correction control efficiency is 52.37%-72.13%.
Bt adds bio-synergistic preparation control diamond-back moth of the present invention, is 3% o'clock containing described bio-synergistic preparation amount of application, and after the dispenser 1 day-7 days, average rate of decrease was 63.74%-83.42%, and the average correction control efficiency is 63.87%-84.69%.Containing described biological potentiating agent amount of application is 5% o'clock, and after the dispenser 1 day-7 days, average rate of decrease was 68.48%-86.71%, and the average correction control efficiency is 68.61%-87.68%.Containing described biological potentiating agent amount of application is 8% o'clock, and after the dispenser 1 day-7 days, average rate of decrease was 68.61%-86.69%, and the average correction control efficiency is 68.70%-87.80%.
Adopt 2% abamectin emulsifiable concentrate to add 3%-8% bio-synergistic preparation control of the present invention diamond-back moth, after the dispenser 7 days, than single average control efficiency raising rate with 2% abamectin emulsifiable concentrate is 5.85%-12.19%, and average correction control efficiency raising rate is 5.26%-10.94%.
Adopt Bt to add 3%-8% biological potentiating agent control of the present invention diamond-back moth, after the dispenser 7 days is 19.48%-24.16% than single average control efficiency raising rate with Bt, and average correction control efficiency raising rate is 17.41%-21.72%.
Adopt duncan's new multiple range method to carry out statistical analysis, the result shows: single control efficiency of handling with 2% abamectin emulsifiable concentrate and the interpolation of 2% abamectin emulsifiable concentrate contain between the control efficiency of bio-synergistic preparation processing of the present invention and have significant difference, it is not remarkable to add between the control efficiency of two processing containing 5% and 8% bio-synergistic preparation of the present invention difference, itself and contain significant difference between the control efficiency that 3% bio-synergistic preparation of the present invention handles, show that bio-synergistic preparation of the present invention has obvious synergistic effect to the control efficiency of biological insecticides 2% abamectin emulsifiable concentrate.
Adopt duncan's new multiple range method to carry out statistical analysis, the result shows: single control efficiency of handling with Bt and Bt add to contain between the control efficiency that bio-synergistic preparation of the present invention handles and have significant difference, it is not remarkable to add between the control efficiency contain two processing of 5% and 8% bio-synergistic preparation of the present invention difference, itself and contain significant difference between the control efficiency that 3% bio-synergistic preparation of the present invention handles, show that bio-synergistic preparation of the present invention has obvious synergistic effect to the control efficiency of biological insecticides Bt.
Do not find in the process of the test crop and non-target beneficial organism are produced harmful effect, safer.
Result of the test shows that bio-synergistic preparation of the present invention has obvious synergistic effect to biopesticide, and quick-acting and lasting effect are good, and to cauliflower safety, the recommendation using dosage is 5%-8%, uses with the biological insecticides mixed atomizing.
Embodiment 2:
Test is formed by following content and component with biological potentiating agent of the present invention,
Fresh egg yolk 15g, edible vegetable oil 45mL, the clear water surplus, cumulative volume is 700mL.
Its compound method is by the preparation method of biological potentiating agent of the present invention " three, " preparation in the technical scheme of the present invention.
Other experimental condition of embodiment 2 is consistent with embodiment 1 with method, is not repeating.
Embodiment 3:
Test is formed by following content and component with biological potentiating agent of the present invention,
Fresh egg yolk 30g, edible vegetable oil 90mL, the clear water surplus, cumulative volume is 1500mL.
Its compound method is by the preparation method of biological potentiating agent of the present invention " three, " preparation in the technical scheme of the present invention.
Other experimental condition of embodiment 3 is consistent with embodiment 1 with method, is not repeating.
2% abamectin emulsifiable concentrate of table 1 2% abamectin emulsifiable concentrate and interpolation biological potentiating agent of the present invention to the diamond-back moth field efficacy relatively
(Yunnan, maritime Yang Guang in November, 2007)
Handle Repeat Radix (head) Handled back 1 day Handled back 3 days Handled back 7 days
Borer population (head) alive Rate of descent (%) Proofread and correct preventive effect (%) Average preventive effect (%) Borer population (head) alive Rate of descent (%) Proofread and correct preventive effect (%) Average preventive effect (%) Borer population (head) alive Rate of descent (%) Proofread and correct preventive effect (%) Average preventive effect (%)
Single 2% abamectin emulsifiable concentrate of using I II III IV 41 35 39 44 13 12 15 14 68.29 65.71 61.54 68.18 68.29 66.46 60.16 69.00 65.98 9 8 10 8 78.05 77.14 74.36 81.82 78.64 77.64 76.01 82.73 78.76 9 6 9 6 78.05 82.86 76.92 86.36 78.64 83.93 80.32 87.36 82.56
2% abamectin emulsifiable concentrate contains 3% biological potentiating agent I II III IV 34 29 43 36 11 9 14 10 67.65 68.97 67.44 72.22 67.65 69.64 66.28 72.93 69.13 6 6 10 7 82.35 79.31 76.74 80.56 82.83 79.76 78.24 81.53 80.59 4 5 6 5 88.24 82.76 86.05 86.11 88.55 83.84 88.10 87.13 86.90
2% abamectin emulsifiable concentrate contains 5% biological potentiating agent I II III IV 38 43 37 40 10 9 8 10 73.68 79.07 78.38 75.00 73.68 79.52 77.61 75.64 76.61 6 5 4 6 84.21 88.37 89.19 85.00 84.64 88.62 89.89 85.75 87.22 5 3 2 5 86.84 93.02 94.59 87.50 87.20 93.46 95.39 88.41 91.12
2% abamectin emulsifiable concentrate contains 8% biological potentiating agent I II III IV 31 28 41 35 7 6 10 9 77.42 78.57 75.61 74.29 77.42 79.04 74.74 74.95 76.54 4 4 5 4 87.10 85.71 87.80 88.57 87.45 86.02 88.59 89.14 87.80 3 3 3 3 90.32 89.29 92.68 90.32 90.58 89.96 93.76 92.06 91.59
Table 2 Bt and the Bt that adds biological potentiating agent of the present invention compare the diamond-back moth field efficacy
(Yunnan, maritime Yang Guang in November, 2007)
Handle Repeat Radix (head) Handled back 1 day Handled back 3 days Handled back 5 days
Borer population (head) alive Rate of descent (%) Proofread and correct preventive effect (%) Average preventive effect (%) Borer population (head) alive Rate of descent (%) Proofread and correct preventive effect (%) Average preventive effect (%) Borer population (head) alive Rate of descent (%) Proofread and correct preventive effect (%) Average preventive effect (%)
Singly use Bt I II III IV 37 44 39 35 19 21 17 17 48.65 52.27 56.41 51.43 48.65 53.31 54.85 52.67 52.37 14 16 14 12 62.16 63.64 64.10 65.71 63.18 64.43 66.42 67.43 65.36 12 14 12 9 67.57 68.18 69.23 74.29 68.44 70.17 73.76 76.17 72.13
Bt contains 3% biological potentiating agent I II III IV 41 28 35 39 15 10 12 15 63.41 64.29 65.71 61.54 63.41 65.06 64.49 62.52 63.87 12 8 9 11 70.73 71.43 74.29 71.79 71.52 72.05 75.94 73.21 73.18 8 4 6 6 80.49 85.71 82.86 84.62 81.02 86.61 85.38 85.74 84.69
Bt contains 5% biological potentiating agent I II III IV 28 38 46 31 8 12 14 11 71.43 68.42 69.57 64.52 71.43 69.11 68.48 65.43 68.61 7 8 9 7 75.00 78.95 80.43 77.42 75.68 79.41 81.70 78.55 78.83 5 6 6 2 82.14 84.21 86.96 93.55 82.63 85.20 88.87 94.02 87.68
Bt contains 8% biological potentiating agent I II III IV 36 29 31 41 11 9 10 13 69.44 68.97 67.74 68.29 69.44 69.64 66.59 69.11 68.70 8 6 6 8 77.78 79.31 80.65 77.78 78.38 79.76 81.89 79.15 79.79 4 4 5 5 88.89 86.21 83.87 87.80 89.19 87.07 86.24 88.70 87.0
CK (clear water) I II III IV 36 45 29 38 36 46 28 39 0.00 -2.22 3.45 -2.63 - - - - - 37 46 31 40 -2.78 -2.22 -6.90 -5.26 - - - - - 37 48 34 41 -2.78 -6.67 -17.24 -7.89 - - - - -
Table 3: singly add the average preventive effect comparison of biological potentiating agent of the present invention to the diamond-back moth field control with 2% abamectin emulsifiable concentrate and 2% abamectin emulsifiable concentrate
Handle Handled back 1 day Handled back 3 days Handled back 7 days Handled back 7 days
Average rate of decrease (%) Average correction control efficiency (%) Average rate of decrease (%) Average correction control efficiency (%) Average rate of decrease (%) Average correction control efficiency (%) Compare with 2% abamectin emulsifiable concentrate with single
Average control efficiency raising rate 1 (%) Average correction control efficiency raising rate 2 (%)
Single 2% abamectin emulsifiable concentrate of using 65.93 65.98 77.84 78.76 81.05 82.56 - -
2% abamectin emulsifiable concentrate contains 3% biological potentiating agent 69.07 69.13 79.74 80.59 85.79 86.90 5.85 5.26
2% abamectin emulsifiable concentrate contains 5% biological potentiating agent 76.53 76.61 86.69 87.22 90.49 91.12 11.65 10.37
2% abamectin emulsifiable concentrate contains 8% biological potentiating agent 76.47 76.54 87.30 87.80 90.93 91.59 12.19 10.94
Table 4: single Bt of using and Bt interpolation biological potentiating agent of the present invention compare the average preventive effect of diamond-back moth field control
Handle Handled back 1 day Handled back 3 days Handled back 7 days Handled back 7 days
Average rate of decrease (%) Average correction control efficiency (%) Average rate of decrease (%) Average correction control efficiency (%) Average rate of decrease (%) Average correction control efficiency (%) Compare with Bt with single
Average control efficiency raising rate 1 (%) Average correction control efficiency raising rate 2 (%)
Singly use Bt 52.19 52.37 63.90 65.36 69.82 72.13 - -
Bt contains 3% biological potentiating agent 63.74 63.87 72.06 73.18 83.42 84.69 19.48 17.41
Bt contains 5% biological potentiating agent 68.48 68.61 77.95 78.83 86.71 87.68 24.19 21.56
Bt contains 8% biological potentiating agent 68.61 68.70 78.95 79.79 86.69 87.80 24.16 21.72
1 calculating is in table 3 and the table 4:
Figure S2007100664553D00101
2 calculating is in table 3 and the table 4:
Figure S2007100664553D00102

Claims (9)

1. improve the biological potentiating agent of biological insecticides control efficiency, it is characterized in that: form by following content and component:
Fresh egg yolk 15-30g,
Edible vegetable oil 45-90mL,
Clear water surplus mL
More than the cumulative volume that closes of each component be 700-1500mL.
2. the described biological potentiating agent of claim 1 is characterized in that: form by following content and component:
Fresh egg yolk 20g,
Edible vegetable oil 60mL,
Clear water surplus mL
More than the cumulative volume that closes of each component be 1000mL.
3. the composition that contains claim 1 or the described biological potentiating agent of claim 2, it is characterized in that: said composition is mixed by biological insecticides and claim 1 or the described biological potentiating agent of claim 2 and is formed, and its described biological potentiating agent consumption is the 3%-8% of the biological insecticides solution usage volume ratio prepared.
4. the described composition of claim 3, it is characterized in that: the biological insecticides in the said composition are 2% abamectin emulsifiable concentrate.
5. the described composition of claim 3, it is characterized in that: the biological insecticides in the said composition are 10,000,000,000 living spores/gram bacillus thuringiensis,Bt wetting powder.
6. the arbitrary described composition that contains biological potentiating agent of claim 3-5 is characterized in that: described biological potentiating agent consumption is 5% of the biological insecticides solution usage volume ratio prepared.
7. the preparation method of claim 1 or the described biological potentiating agent of claim 2, it is characterized in that: this method may further comprise the steps:
(a) take out the clear water rinsing of fresh egg yolk, get yolk 15-30g;
(b) yolk and proper amount of clear water are stirred, add edible vegetable oil 45-90mL again, stir, add clear water at last to 700-1500mL, and mix, not stratified in clear water until the preparation of making, can fully be dissolved into the emulsion of milk shape, place cold compartment of refrigerator standby.
8. the arbitrary described application that contains the composition of biological potentiating agent of claim 3-6 is characterized in that: carry out spray application at insect pest initial stage of origination or Sheng phase.
9. the described application that contains the composition of biological potentiating agent of claim 8 is characterized in that: the control of the diamond-back moth insect on the cauliflower crop is used.
CNA2007100664553A 2007-12-14 2007-12-14 Biological potentiating agent for increasing preventing and controlling effect of living insecticide, composite, preparation method and application Pending CN101199292A (en)

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CN103987263A (en) * 2011-12-09 2014-08-13 金承穆 Pest control composition and method for preparing the same
CN104068067A (en) * 2014-06-20 2014-10-01 浙江省农业科学院 Application of yolk and edible oil mixture to production of reagent for preventing and treating crop diseases and pests
CN107306946A (en) * 2017-05-10 2017-11-03 中国农业大学 A kind of egg oil agricultural chemicals and its preparation method and application
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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CN103987263A (en) * 2011-12-09 2014-08-13 金承穆 Pest control composition and method for preparing the same
CN103987263B (en) * 2011-12-09 2016-03-16 金承穆 Control of insect composition and manufacture method thereof
CN103975960A (en) * 2014-04-04 2014-08-13 秦皇岛市远航有机肥料技术开发有限公司 Green organic insect-expelling insecticide for crops
CN104068067A (en) * 2014-06-20 2014-10-01 浙江省农业科学院 Application of yolk and edible oil mixture to production of reagent for preventing and treating crop diseases and pests
CN104068067B (en) * 2014-06-20 2017-01-25 浙江省农业科学院 Application of yolk and edible oil mixture to production of reagent for preventing and treating crop diseases and pests
CN107306946A (en) * 2017-05-10 2017-11-03 中国农业大学 A kind of egg oil agricultural chemicals and its preparation method and application
CN108849885A (en) * 2018-08-07 2018-11-23 广州市新景象生态科技有限公司 The agricultural synergist and preparation method thereof of one kind of multiple vegetable oil combinations

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