CN101180963A - Composition containing Zhongshengmycin and thiophanate-methyl with synergism - Google Patents
Composition containing Zhongshengmycin and thiophanate-methyl with synergism Download PDFInfo
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- CN101180963A CN101180963A CNA2007103002403A CN200710300240A CN101180963A CN 101180963 A CN101180963 A CN 101180963A CN A2007103002403 A CNA2007103002403 A CN A2007103002403A CN 200710300240 A CN200710300240 A CN 200710300240A CN 101180963 A CN101180963 A CN 101180963A
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- rhzomorph
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Abstract
The synergistic composition of C19H34O8N8 and triophanate-methyl relates to a pesticide composition. The active ingredients are C19H34O8N8 and triophanate-methyl. The composition is used for the prevention and treatment of disease of the apple tree, particularly for the prevention and treatment of the apple ring rot.
Description
Technical field
The composition pesticide that this invention relates to is to be active ingredient with middle living rhzomorph and thiophanate-methyl, is used to prevent and treat ring rot of apple on agricultural.
Technical background
The middle rhzomorph of giving birth to is a kind of novel bactericide.Belong to a kind of N-glucoside antibiotic.Its has a broad antifungal spectrum all has good result to various bacteria, fungi.Gram-negative and positive bacterium there is very good effect.Can cause in the hyphal cell protoplasm cohesion to fungi, the sprouting of pathogen spore is had had strong inhibitory effects.There is not cross resistance with conventional dose.
Thiophanate-methyl is a benzimidazole germicide.The thiophanate-methyl fungicidal spectrum is wide, and multiple diseases is all had the better prevention effect.Have interior suction, prevention and therapeutic action.It is converted into carbendazim in plant corpus, the formation of spindle influences cell division in the mitosis of interference germ.
Yet in the real process of agricultural production, preventing and treating the problem that disease is easy to generate most is the drug-fast generation of germ.Most important disease is a ring rot of apple in apple cultivation process, because its spread speed is fast, it is serious to cause harm, as prevents and treats untimelyly, often causes early stage a large amount of fallen leaves, seriously undermines tree vigo(u)r, influences output.Preventing and treating this disease often needs a large amount of agricultural chemicals that use, so be easy to generate resistance especially.In some ring spots serious place of causing harm, common bactericide such as tpn, carbendazim, use has not had any effect separately.Medicaments such as thiophanate-methyl have been used in a lot of places in a large number because long-time, large-area application, be relatively be easy to generate drug-fast.
Go up the disease that produces resistance for control agricultural, a kind of way is to release the new component that new and existing kind do not have cross resistance.But, the development cost height of new active ingredient, the construction cycle is long, and all produces the speed of resistance forever less than germ.Other method such as crop allocation adjustment, different agricultural chemicals are rotated etc., in the process of practical operation, are difficult to really play obvious effects.
The composition of different cultivars carries out composite, is to prevent and treat the very common way of resistance germ.Heterogeneity is carried out composite, according to the effect of practical application, judge certain composite be synergy, add and or antagonism.Compound synergic is well filled a prescription, owing to obviously improved actual control efficiency, reduced the usage amount of agricultural chemicals, thereby delayed the drug-fast generation of germ greatly, and be the important means of integrated control disease.Still nobody reported to give birth to the composite of rhzomorph and thiophanate-methyl and application at present.
Summary of the invention
Go up the germ that produces resistance in order to prevent and treat agricultural, we have carried out mutual composite synergy research with compositions such as middle living rhzomorph, thiophanate-methyls.It is tested object that ring rot of apple is adopted in test, and concrete grammar is:
Test material is the ring rot of apple germ.Test medicine provides former medicine for producer, take by weighing a certain amount of middle former medicine of rhzomorph, the former medicine of thiophanate-methyl of giving birth to, in give birth to the direct water dissolving of rhzomorph, thiophanate-methyl add a certain amount of dimethyl sulfoxide (DMSO) with its dissolving after, add Tween-80 more respectively and a certain amount of aqua sterilisa is made into mother liquor.On the basis of mother liquor, by the trial test result, series is for the dilution of examination concentration more than 5 in preparation, and each concentration is established more than 4 and repeated.By the preliminary experiment result, respectively according to middle living rhzomorph: thiophanate-methyl is 1: 2,1: 5,1: 10,1: 20,1: 40 proportioning, with aqua sterilisa mother liquor is diluted to suitable series concentration.
The mycelial growth rate method is adopted in test.Compare (CK) with the sterile water solution that contains an amount of dimethyl sulfoxide (DMSO) and Tween 80.Getting the 2ml soup joins in the conical flask that the hot medium of 38ml (PDA medium, 45-50 ℃) is housed, after shaking up, pour diameter 90mm glass culture dish rapidly into, each culture dish is poured band medicine medium 13ml into, and each handles three repetitions, level leaves standstill, and makes flat board after the cooling.One of the bacterium cake of every ware access diameter 0.4cm.All operations all carries out sterile working at superclean bench.Be placed on after the processing in 26 ± 1 ℃ the constant temperature aseptic culture case and cultivate, take out behind the 3d.Adopt the right-angled intersection method to measure the colony diameter that each is handled respectively, the mean value, the mycelial growth that calculate colony diameter suppress virulence regression equation and EC
50
Calculate the toxicity index of each medicament and the co-toxicity coefficient of mixture (CTC value) according to the abundant method of Sun Yun, promptly CTC≤80 are antagonism, and 80＜CTC＜120 are summation action, and CTC 〉=120 are synergistic effect.Co-toxicity coefficient (CTC)=mixture actual measurement toxicity index (the ATI)/theoretical toxicity index (TTI) of mixture.
Actual measurement toxicity index (ATI)=standard medicament EC
50/ reagent agent EC
50* 100
The percentage composition of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture
The percentage composition of B in+B medicament toxicity index * mixture
Test be mixed result such as following table:
Give birth to the prescription The selection result of rhzomorph thiophanate-methyl in the table 1 to the ring rot of apple bacterium
| Reagent agent | Slope (B) | Intercept (A) | Correlation coefficient (R) | EC 50(mg/L) | Mixture actual measurement virulence refers to ATI) | The theoretical toxicity index (TTI) of mixture | The CTC value |
| Z (middle living rhzomorph) | 5.2516 | 1.2900 | 0.9968 | 0.64 | |||
| J (thiophanate-methyl) | 2.8489 | 1.4900 | 0.9953 | 27.78 | |||
| ZJ 1(1∶1) | 5.0255 | 1.1359 | 0.9862 | 0.95 | 67.21 | 51.15 | 131.41 |
| ZJ 2(1∶2) | 4.9924 | 1.2812 | 0.9912 | 1.01 | 62.96 | 52.30 | 120.38 |
| ZJ 3(1∶5) | 5.1877 | 1.3806 | 0.9921 | 0.73 | 87.29 | 55.74 | 156.58 |
| ZJ 4(1∶10) | 5.2421 | 1.2445 | 0.9895 | 0.64 | 99.90 | 61.49 | 162.47 |
| ZJ 5(1∶20) | 5.5875 | 1.6215 | 0.9935 | 0.43 | 147.00 | 72.98 | 201.43 |
| ZJ 5(1∶40) | 5.5680 | 1.6003 | 0.9911 | 0.44 | 144.53 | 95.95 | 150.62 |
Experimental result is mixed:
Middle living rhzomorph and thiophanate-methyl are mixed, and the ring rot of apple germ is had good indoor activity.Middle rhzomorph and six proportionings of thiophanate-methyl of giving birth to, its CTC value is all greater than 120.Show that two effective ingredients have synergistic effect under this this proportioning.By being mixed, can reach the effect that reduces cost, improves the medicament activity and delay the generation of pathogen pesticide resistance.
Embodiment
Application example one: ring rot of apple
By middle living rhzomorph 1%, thiophanate-methyl 25%, dispersant calcium lignosulfonate 8%, wetting agent neopelex 3%, surplus adds kaolin, breaks into 600 purpose wetting powders with airslide disintegrating mill.This prescription is by 1000 times of dilution sprayings, and 14 days control efficiency are to give birth to rhzomorph wetting powder and 70% thiophanate-methyl wetting powder in 96.2%, 3% according to using with quadrat method behind the medicine, and 14 days preventive effects are respectively 83.3% and 82.0% behind the medicine.
Application example two: ring rot of apple
By middle living rhzomorph 2%, thiophanate-methyl 20%, dispersant sodium lignin sulfonate 10%, wetting agent neopelex 3%, surplus adds kaolin, breaks into 600 purpose wetting powders with airslide disintegrating mill.This prescription is handled with 1500 times of dilution sprayings.14 days preventive effects reach 90.0% behind the medicine.Give birth to rhzomorph wetting powder and 70% thiophanate-methyl wetting powder in 3% according to using with quadrat method.14 days control efficiency are respectively 72.2% and 75.5% behind the medicine.
Claims (3)
1. composition pesticide, effective ingredient is made up of middle living rhzomorph and thiophanate-methyl, and the mass ratio of middle living rhzomorph and thiophanate-methyl is 1: 1-1: between 40.
2. the composition of mentioning in the claim 1 is made wetting powder during practical application.
3. the composition of mentioning in the claim 1, the mass percentage content of effective ingredient in preparation is between 1%-80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007103002403A CN101180963B (en) | 2007-12-17 | 2007-12-17 | Composition containing Zhongshengmycin and thiophanate-methyl with synergism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007103002403A CN101180963B (en) | 2007-12-17 | 2007-12-17 | Composition containing Zhongshengmycin and thiophanate-methyl with synergism |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101180963A true CN101180963A (en) | 2008-05-21 |
| CN101180963B CN101180963B (en) | 2012-04-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2007103002403A Active CN101180963B (en) | 2007-12-17 | 2007-12-17 | Composition containing Zhongshengmycin and thiophanate-methyl with synergism |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102369940A (en) * | 2011-10-31 | 2012-03-14 | 陕西美邦农药有限公司 | Bactericidal composite containing fluopicolide and Zhongshengmycin |
| CN104488930A (en) * | 2014-12-18 | 2015-04-08 | 广西田园生化股份有限公司 | Compound composition containing methanesulphonyl myclobutanil and zhongshengmycin and preparation |
| CN106342851A (en) * | 2016-08-31 | 2017-01-25 | 福建凯立生物制品有限公司 | Composition of Zhongshengmycin, pyrimethanil and thiophanate methyl |
| CN106922712A (en) * | 2015-12-30 | 2017-07-07 | 江苏龙灯化学有限公司 | A kind of bactericidal composition |
| CN107459398A (en) * | 2017-08-31 | 2017-12-12 | 江西省农业科学院植物保护研究所 | A kind of medicine fertilizer and preparation method thereof, application process, purposes |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1219879C (en) * | 2003-11-04 | 2005-09-21 | 中国农业科学院生物防治研究所 | Hainan variety of lilac grey streptomycete and method for preparing agricultural antibiotic from variety |
| CN1267007C (en) * | 2003-11-05 | 2006-08-02 | 中国农业科学院生物防治研究所 | Antibiotic agricultural chemicals wettable powder and its preparation method |
| AR049371A1 (en) * | 2004-03-15 | 2006-07-26 | Basf Ag | FUNGICIDE MIXTURES, FUNGICIDE PRODUCT CONTAINING THEM, SEEDS WITH AGGREGATE OF THE MENTIONED MIXTURE AND A PROCEDURE TO COMBAT POTENTIAL NOISE FUNGIANS OF THE RICE THAT USES AN ACTIVE SYNTHETIC AMOUNT OF THE MIXTURE |
-
2007
- 2007-12-17 CN CN2007103002403A patent/CN101180963B/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102369940A (en) * | 2011-10-31 | 2012-03-14 | 陕西美邦农药有限公司 | Bactericidal composite containing fluopicolide and Zhongshengmycin |
| CN104488930A (en) * | 2014-12-18 | 2015-04-08 | 广西田园生化股份有限公司 | Compound composition containing methanesulphonyl myclobutanil and zhongshengmycin and preparation |
| CN106922712A (en) * | 2015-12-30 | 2017-07-07 | 江苏龙灯化学有限公司 | A kind of bactericidal composition |
| CN106342851A (en) * | 2016-08-31 | 2017-01-25 | 福建凯立生物制品有限公司 | Composition of Zhongshengmycin, pyrimethanil and thiophanate methyl |
| CN107459398A (en) * | 2017-08-31 | 2017-12-12 | 江西省农业科学院植物保护研究所 | A kind of medicine fertilizer and preparation method thereof, application process, purposes |
| CN107459398B (en) * | 2017-08-31 | 2020-10-09 | 江西省农业科学院植物保护研究所 | Pesticide fertilizer and preparation method, application method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101180963B (en) | 2012-04-18 |
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