CN101074434A - Special composite enzyme for breaking wall and decomposing plant pollen cell - Google Patents
Special composite enzyme for breaking wall and decomposing plant pollen cell Download PDFInfo
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- CN101074434A CN101074434A CN 200610026529 CN200610026529A CN101074434A CN 101074434 A CN101074434 A CN 101074434A CN 200610026529 CN200610026529 CN 200610026529 CN 200610026529 A CN200610026529 A CN 200610026529A CN 101074434 A CN101074434 A CN 101074434A
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- Prior art keywords
- enzyme
- pollen
- special composite
- cell wall
- wall breaking
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Abstract
A special composite enzyme for decomposing and breaking the wall of vegetable pollen cell consists of beta-glucanase 15-30 wt%, pankrin 15-30 wt%, chitinase 20 wt% and lipase 20 wt%. The process is carried out by mixing pollen with water at 50-60 degree and pH=5.6-6.6 in proportion of 1:3, dissolving special composite enzyme and water in proportion of 1:10, adding into pollen solution, agitating, keeping temperature at 55 degree for 20-40 hrs, enzyme decomposing, raising temperature while killing enzyme, filtering to obtain clarified liquid, spraying and drying to obtain final product. It has composite formula with multiple enzyme preparations and better health-care and medicinal functions.
Description
Technical field:
The present invention relates to bioengineering field, belong to biological medicine extracts active ingredients technology, particularly the plant pollen cell wall breaking decomposes special composite enzyme and application thereof
Background technology:
At present plant pollen is absorbed natural estrogen as the medicines and health protection based food by the feed mode, generally adopt pollen and honey mixed edible or pollen is pressed into tablet and use.In a word, the application of pollen is the application under the state that is not cracked of the cell at pollen.Because the pollen cell wall of plant is to be made of chitosan class material, the pollen cell shell is hard unusually, and is edible under the situation that cell walls is not decomposed by collapse, can't be absorbed by the human body normal stool, so can't reach the result of use of expection at all.Destroy and decompose the key that the pollen cell wall is a raising pollen using value, traditional cell wall breaking mode is following mode: the pollen cell wall is abolished in (1) mechanical disintegration friction, and (2) ultrasonic wave mode is destroyed the pollen cell wall.But also in the processing of pollen, do not use with upper type,, reduced the range of application of pollen even adopt the desired result that can not reach the completely destroy pollen cell.Decompose special composite enzyme with a kind of plant pollen cell wall breaking and adopt water decomposition pollen cell wall to discharge the mode of content material, will greatly improve the use value of plant pollen.
One of purpose of the present invention is the deficiency that overcomes above-mentioned technology, provides a kind of plant pollen cell wall breaking to decompose special composite enzyme
Another object of the present invention provides the application method that a kind of plant pollen cell wall breaking decomposes special composite enzyme
Of the present invention according to following scheme realization:
A kind of plant pollen cell wall breaking decomposes special composite enzyme, it is characterized in that it is the prozyme that the one-tenth by the per-cent of following weight is grouped into
Beta glucan enzyme 15%-30%
Proteolytic enzyme 15%-30%
Chitoanase 20%
Lipase 20%
Above-mentioned beta glucan enzyme is made of the composition of the per-cent of following weight:
Beta glucan enzyme 40%
Cellulase 40%
Pentosanase 20%
Above-mentioned proteolytic enzyme is made of the composition of the per-cent of following weight:
Inscribe peptase 50%
Carboxypeptidase 25%
Aminopeptidase 25%
Above-mentioned lipase is made of the composition of the per-cent of following weight
Lipase 60%
Pectinesterase 40%.
Claims (6)
1. a plant pollen cell wall breaking decomposes special composite enzyme, it is characterized in that it is the prozyme that the one-tenth by the per-cent of following weight is grouped into
Beta-glucanase 15%-30%
Proteolytic enzyme 15%-30%
Chitoanase 20%
Lipase 20%.
2. plant pollen cell wall breaking as claimed in claim 1 decomposes special composite enzyme, it is characterized in that above-mentioned beta-glucanase is made of the composition of the per-cent of following weight:
Beta-glucanase 40%
Cellulase 40%
Pentosanase 20%.
3. plant pollen cell wall breaking as claimed in claim 1 decomposes special composite enzyme, it is characterized in that above-mentioned proteolytic enzyme is made of the composition of the per-cent of following weight:
Inscribe peptase 50%
Carboxypeptidase 25%
Aminopeptidase 25%.
4. plant pollen cell wall breaking as claimed in claim 1 decomposes special composite enzyme, it is characterized in that above-mentioned lipase is made of the composition of the per-cent of following weight
Lipase 60%
Pectinesterase 40%.
5. plant pollen cell wall breaking as claimed in claim 1 decomposes special composite enzyme, it is characterized in that above-mentioned chitoanase is a chitoanase.
6. plant pollen cell wall breaking as claimed in claim 1 decomposes the application method of special composite enzyme, it is characterized in that pollen and temperature 50 degree-60 degree, the warm water of pH value 5.6-6.6 mixes, the ratio of pollen and water is 1: 3 times, described plant pollen cell wall breaking is decomposed in the aqueous solution of special composite enzyme and water dissolving back adding in 1: 10 pollen raw material and stir, zymolytic raw material need keep temperature 55 degree 20-40 hour by the time enzyme decompose pollen raw material fully and reach expectation index, pollen raw material heats up to go out and enters that to obtain the clear liquid spraying drying after filterer's preface be product behind the enzyme.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610026529 CN101074434A (en) | 2006-05-15 | 2006-05-15 | Special composite enzyme for breaking wall and decomposing plant pollen cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610026529 CN101074434A (en) | 2006-05-15 | 2006-05-15 | Special composite enzyme for breaking wall and decomposing plant pollen cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101074434A true CN101074434A (en) | 2007-11-21 |
Family
ID=38975706
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610026529 Pending CN101074434A (en) | 2006-05-15 | 2006-05-15 | Special composite enzyme for breaking wall and decomposing plant pollen cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101074434A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102948656A (en) * | 2012-10-26 | 2013-03-06 | 北京知蜂堂蜂产品有限公司 | Method for wall breaking, desensitization and water-soluble component extraction of pollen and water-soluble pollen cream prepared by the method |
| CN102948496A (en) * | 2012-10-26 | 2013-03-06 | 北京知蜂堂蜂产品有限公司 | Method for pollen wall breaking by enzyme and extraction of pollen grease composition by supercritical CO2 |
-
2006
- 2006-05-15 CN CN 200610026529 patent/CN101074434A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102948656A (en) * | 2012-10-26 | 2013-03-06 | 北京知蜂堂蜂产品有限公司 | Method for wall breaking, desensitization and water-soluble component extraction of pollen and water-soluble pollen cream prepared by the method |
| CN102948496A (en) * | 2012-10-26 | 2013-03-06 | 北京知蜂堂蜂产品有限公司 | Method for pollen wall breaking by enzyme and extraction of pollen grease composition by supercritical CO2 |
| CN102948656B (en) * | 2012-10-26 | 2014-04-09 | 北京知蜂堂蜂产品有限公司 | Method for wall breaking, desensitization and water-soluble component extraction of pollen and water-soluble pollen cream prepared by method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20071121 |