CN101049074A - Breeding and reproducing eucalyptus type cajeput with alternate leaves - Google Patents
Breeding and reproducing eucalyptus type cajeput with alternate leaves Download PDFInfo
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- CN101049074A CN101049074A CN 200710027978 CN200710027978A CN101049074A CN 101049074 A CN101049074 A CN 101049074A CN 200710027978 CN200710027978 CN 200710027978 CN 200710027978 A CN200710027978 A CN 200710027978A CN 101049074 A CN101049074 A CN 101049074A
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Abstract
A method for culture and reproducing the eucalyptus-kind cajeputtree whose content of 1, 8-eucalyptol is more than 70% includes such steps as choosing strong cajeputtree plant, culturing it in big plastic film shed, choosing by several generations according to the content of 1, 8-eucalyptol until obtaining an excellent cajeputtree plant whose content of 1, 8-encalyptol is more than 70%, using it as female parent, and culturing and reproducing by cuttage method or tissue culture method.
Description
Technical field
The present invention relates to a kind of method of the new varieties that cultivate plants, specifically is cultivation and numerous method for planting of narrow leaved tea tree new variety of plant.
Background technology
Narrow leaved tea tree is Australia cajeputtree again, formal name used at school: Melaleuca alternifolia, Myrtaceae (Myrtaceae), Melaleuca (MelaleucaL.).Cajeputtree original producton location, Australia is an Australia, aiphyllium, narrow leaf, its height of tree can reach 6 meters, deep-rootedness, happiness acid ground, drought-resistant dead soil of energy and accumulated water ground, the area growth of growing in temperature height, frost-free season is rapid, and the ability light frost has certain cold resistance.Common cajeputtree plant variety has: narrow leaved tea tree belongs to kind of (melaleucaaltemifolia); Bag squama cajeputtree kind (melaleuca bracteata): Chinese Photinia Leaf cajeputtree kind (melaleuea eriefolia); White oil seeds (melaleuea quinquenerria); Niaouli kind (melaleueaviridiflora); Cajeputtree cajaput kind melaleuca leueadendra) and cajeputtree seeds (melaleuca cajapnti) etc. multiple, the leaf shape has needle and Liu Zhuanye.The branches and leaves of can gathering after narrow leaved tea tree was planted 1 year extract essential oil, can go into full operation after 1 year, and every strain can be adopted branches and leaves 1.5-2.5 kilogram every year, can gather in oil refining 1-2 time every year, and bright branches and leaves oil yield is about 1.0%, is the very high economic crops of economic worth.The aromatic oil that its branches and leaves extract promptly claims to contain 10%1 in the world, and that the 8-cineole is following is " tea oil " (Tea Tree Oil), through after the deep processing, 1, what the 8-cineole reached 70-80% is called 1, the 8-eucalyptus oil, more than 90% is 1, the 8-cineole.Tea oil (eucalyptus oil) is the stronger natural antibacterial agent of present known activity, can kill the fungi and the bacterium on human body skin surface efficient, nontoxic, non-stimulatedly, and its sterilizing power is a standard bactericide-phenolic 13 times.FDA (FDA) authorization tea oil need not handle with regard to vendible first-aid medicine and health products as a kind of.1, the 8-cineole is very good in the international market, and it mainly contains terpinene-4 ethanol, 1, and more than ten kind of compositions such as 8-cineole, γ-Song Youxi are rare natural health pharmaceuticals materials.Can be widely used in spicing industry, perfume industry, cosmetic industry and soapmaking industry, can be used as preservative, analeptic again, particularly importantly it can be used as medicine chemical material.
In recent years, introduce a fine variety and establishing in large scale in succession in Guangdong, Guangxi, Fujian.But because the seed of narrow leaved tea tree mostly is abortion, germination rate is low, and interspecific difference is big, causes the good seed scarcity, has restricted the raising of essential oil production quantity and product quality.On output,, (mainly refer to 1,8-cineole and Foxlene-1 alcohol-4) qualitatively also not as good as Australian tea oil at essential oil far away from Australia.According to ISO 4730 (international standard) regulation, terpinenol .4 〉=30%, 1 of narrow leaved tea tree essential oil, 8-cineole≤15%.AS 2782 (Australian national standard) regulation, terpinenol-4 〉=35%, 1,8-cineole≤4% ± 2%.Mainly to wherein terpinenol-4 and 1,8-cineole content is limited for these standards and regulation.As seen terpinenol .4 and 1, the 8-cineole is the characteristic component of this plants essential oil, whether good the height that contains the alcohol amount be to weigh one of narrow leaved tea tree essential oil sign.Though some domestic enterprises also recognize the effect of tea oil, certain exploitation is also arranged on product, but still just be confined to aspects such as perfumed soap, bath foam, conditioning liquid, toothpaste, mildy wash.In pharmaceuticals industry, food flavor industry almost is blank.Some use tea oil also very little as enterprise's consumption of raw material, secondly, because the narrow leaved tea tree that China's seed is introduced, a plantation year per mu yield has only the 2-3 ton, the yield of carrying of essential oil has only 0.7-0.9%, so that processing cost is higher, the costing an arm and a leg of essential oil, the price of its deep processed product is also corresponding higher.The narrow leaved tea tree kind 1 of the overwhelming majority, 8-cineole content is below 10%, and high-load then must carry out deep processing if desired, and the process complexity causes processing cost higher, the costing an arm and a leg of essential oil, the price of its deep processed product is also corresponding higher.
It is investigated that domestic data shows, the output of China's eucalyptus oil is along with raw-material continuous atrophy shortage sharply descends year by year.Substantially, be in blank aspect the outlet sale in recent years, the eucalyptus oil of domestic use is mainly by import.Domestic production at present has the eucalyptus oil of minute quantity, its main source: the one, and the Eucalyptus Globulus oil that produce in Yunnan; The 2nd, the fragrant camphor oil that produce in Sichuan; The 3rd, the assorted camphor oil that produce in Jiangxi, Hunan, Guangdong, Guangxi.The Eucalyptus Globulus oil in Yunnan is owing to cut down the blue gum steam oil for a long time, and the age of tree is aging mostly, and the blue gum of planting could be cut down steam oil after more than at least 10 years, so Eucalyptus Globulus oil output descended year by year in recent years.And the assorted camphor oil that produce in Jiangxi, Hunan, Guangdong, Guangxi is that the felling camphor tree is got its root and comes steam oil, and is very big to the destructiveness of the woods.National forestry portion hereinafter forbids cutting down the camphor tree steam oil.So one of source of eucalyptus oil is blocked again.This shows that in quite general period, eucalyptus oil is in the situation that supply falls short of demand.And eucalyptus oil is traditional commodities at home, and it is the narrow leaved tea tree essential oil of Main Ingredients and Appearance that market demand is far longer than with 4-terpineol (teypinen-4-01).On the marketability price ratio, eucalyptus oil also exceeds about 20,000 yuan than narrow leaved tea tree essential oil price per ton at home.In view of the above, eucalyptus type narrow leaved tea tree kind has more the market development potential.The narrow leaved tea tree seeds are carried out breed breeding to inventor's decision and exploitation is grown in training.
Summary of the invention
The purpose of this invention is to provide a kind of 1, the cultivation and the propagation method of the new variety of plant of the eucalyptus type narrow leaved tea tree kind that 8-cineole content is higher.
For being planted out in each province, China south, narrow leaved tea tree contains the high kind of essential oil amount, the inventor is through in a few years to research and the test of narrow leaved tea tree seeds, introduce in many kinds of planting in flakes at seed, repeatedly the narrow leaved tea tree plant variety is compared and screens, screening is also cultivated tree-like sturdy, tree crown is bigger, be thick with leaves, fast growth, and bright branches and leaves oil yield can be up to 1.0-1.8%, in the essential oil 1,8-cineole content is up to the eucalyptus type narrow leaved tea tree kind more than 70%, and the leaf that we cultivate this kind is shaped as needle-like, present domestic and international natural seeds 1 have been filled up, the blank that 8-cineole content is lower.
Technical scheme of the present invention is as follows:
Tens narrow leaved tea tree seeds that the inventor at first will introduce for many years, analyze the chemical composition of seeds, regather the seed that contains the folium eucalypti innage, general 1,8-cineole content reaches more than 50% (high more good more) and screens, adopt ultraviolet illumination, with disinfectant seed soaking 10-30 minute, will damage then, full or not different in nature seed is removed, in booth, cultivate, select tree-like sturdy, tree crown is bigger, and the tree type that is thick with leaves extracts 1,8-cineole content, preferred through several generations, obtain 1 of individual plant, 8-cineole content as female parent, adopts following two kinds of methods to cultivate and breed up to the improved seeds more than 70% then:
Method one: the annual semi-lignified of fine individual plant, health, disease-free branch → bud induce propagation → cultivation to strengthen bud → sprigging.
Method two: the sturdy mutual leaf → callus → bud of fine individual plant annotinous branch induces propagation → cultivation to strengthen bud → sprigging.
It is branch or sturdy mutual leaf callus to be put in the inducing solution cultivate that described bud is induced enrichment procedure, and consisting of of this culture fluid can be following several any one:
(1) mixed solution of the indole-3-butyric acid of 0.1-0.5mg/L (IBA)+0.1-0.5mg/L methyl (NAA), the ABT1-9 of 0.1-0.5mg/L number wherein a kind of root-inducing powder also adds agar 3-10g/L in the medium, sucrose 10-40g/L, 1,8-cineole 1-5g/L, pH5.5-6.5.
(2) 2,4-dichlorphenoxyacetic acid (2,4-D) the mixed solution of 0.1-0.5mg/L, indole-3-butyric acid (IBA) 0.1-0.5mg/L 0.1-0.5mg/L methyl (NAA), 0.1-0.5mg/L ABT1-9 number wherein a kind of root-inducing powder, also add agar 3-10g/L in the medium, sucrose 10-40g/L, 1,8-cineole 1-5g/L, pH5.5-6.5.
(3) mixed solution of TDZ0.1-0.5mg/L, indole-3-butyric acid (IBA) 0.1-0.5mg/L 0.1-0.5mg/L methyl (NAA), 0.1-0.5mg/L ABT1-9 number wherein a kind of root-inducing powder, also add starch 3-10g/L in the medium, glucose 10-40g/L, 1,8-cineole 1-5g/L, pH5.5-6.5.
(4) mixing material of MS medium 0.1-0.5mg/L, benzyl purine (BA) 0.1-0.5mg/L, methyl (NAA) 0.1-0.5mg/L, 0.1-0.5mg/L ABT1-9 number wherein a kind of root-inducing powder, also add starch 3-10g/L in the medium, glucose 10-40g/L, 1,8-cineole 1-5g/L, pH5.5-6.5.
(5) mixing material of MS medium 0.1-0.5mg/L, indole-3-butyric acid (IBA) 0.1-0.5mg/L, methyl (NAA) 0.5-1.0mg/L, 0.1-0.5mg/L ABT 1-9 number wherein a kind of root-inducing powder, also add agar 3-10g/L in the medium, sucrose 10-40g/L, 1,8-cineole 1-5g/L, pH5.5-6.5.
(6) mixed solution of N5 medium 0.1-0.8mg/L, indole-3-butyric acid (IBA) 0.1-0.8mg/L benzyl purine (BA) 0.1-0.8mg/L, 0.1-0.5mg/L ABT1-9 number wherein a kind of root-inducing powder, also add agar 3-10g/L in the medium, sucrose 10-40g/L, 1,8-cineole 1-5g/L, pH5.5-6.5.
(7) mixed solution of 1/2MS medium, IBA 0.1-0.6mg/L, the ABT1-9 of 1.0-2.0mg/L number wherein a kind of root-inducing powder also adds starch 3-10g/L in the medium, glucose 10-40g/L, 1,8-cineole 1-5g/L, pH5.5-6.5.
The method of cultivating is: the eucalyptus type narrow leaved tea tree sprigging that above-mentioned two kinds of methods are obtained, because bud induces proliferating liquid to contain 1, the 8-cineole, the eucalyptus type narrow leaved tea tree 1 of the first generation, 8-cineole content is brought up to about 55% from 50%, the eucalyptus type narrow leaved tea tree that the content that the first generation is obtained is high is again organized training or cuttage once more, 1,8-cineole content can improve about 5% again, all add at every turn and contain 1, the bud of 8-cineole is induced training of proliferating liquid group or cuttage, and the cultivation of passing through some generations just obtains 1 more than 75%, the improved seeds of 8-cineole.
Eucalyptus type narrow leaved tea tree of the present invention can be from 1, the mechanism that 8-cineole content is brought up to more than 75% from 50% plain edition is that each all adds a certain amount of 1 for inducing at the bud of cultivating tissue cultivating seedling or cuttage seeding seedling process the proliferating liquid, the 8-cineole, according to the biochemical reaction principle, the reactant that adds contains certain material, product will be with the heredity of going up this material, particularly 1 of adding of the present invention, 8-cineole and eucalyptus type narrow leaved tea tree extract itself are with a kind of material, follow-on like this nursery stock contains 1, the 8-cineole can improve, having arrived some generations just can be so that hangs down 1 originally, the narrow leaved tea tree of 8-cineole content is elevated to desired content, and settles out gradually, forms new strain, this time is as long as adopt vegetative propagation, no longer cultivate of future generationly with the method for planting seed, just can guarantee in the narrow leaved tea tree 1,8-cineole content remains unchanged.
In the process of cultivating eucalyptus type narrow leaved tea tree of the present invention, the inventor also finds, when the cuttage of eucalyptus type narrow leaved tea tree, along with kind 1, the raising of 8-cineole content, survival rate reduces gradually, by analysis, find in the branch 1,8-cineole high-load has influenced the germination of narrow leaved tea tree callus, according to common way, begins us and adopts 1 of method flush away wound that clear water soaks, the 8-cineole, it is also very limited that but survival rate improves, and reason is that clear water is not easy the oil dissolving, and the time has been grown the microbial attack wound, cause corruption, if do not contact with soil besides plant passes through several hrs, just again be difficult to survive, we adopt organic solvent to soak the cuttage branch through test of many times, make the wound tissue of branch adsorb bud easily and induce the material of taking root in the proliferating liquid, take root in the cuttage process easily.The organic solvent that the present invention adopts at lauryl sodium sulfate, aliphatic alcohol sulfate, methyl alcohol, ethanol, is selected in the materials such as propyl alcohol, isopropyl alcohol, ether, ethylene glycol, and mass concentration is 1-10%.
Cuttage and seedling culture method of the present invention:
Select the tender sprout of sturdy, semi-lignified of the sapling (transplant land for growing field crops with tissue cultivating seedling then grow up to) of life in 1 year, about length 13-15 centimetre, earlier soak branch Lower Half (cuttage bury part) 3-5 hour (because of the shoot oil content height of eucalyptus type narrow leaved tea tree with the 1-10% organic solvent, the purpose of soaking is de-oiling, in order to taking root).Then sterilized 1-5 minute with the potassium permanganate of 0.1-1.0%; Then, the bud that placement prepares is in advance induced in the proliferating liquid and is handled, and gets final product cuttage.Will be before the cuttage with the nutritive mud in the nutritious bag, 7-10 days is formalin (formaldehyde) thorough disinfection of 1-5% with concentration in advance, and the cuttage degree of depth is long 1/3 being advisable of branch, and branch is moistening gets final product in maintenance after the cuttage.The cuttage of eucalyptus type narrow leaved tea tree is in planting percent the best in April to October in every year.Took root in general 20 days, 2 wheat harvesting periods can be planted, and emergence rate is more than 90%.Cuttage seeding survives transplanting land for growing field crops, back and detects after 3 years, keeps the fine quality of former fine individual plant equally.
The method of of the present invention group of training is:
With preferred narrow leaved tea tree leaf nose part, through disinfecting (sterilization method is identical with the sterilization method of common plant tissue culture seedling), what adding prepared in advance then contains 1, the bud of 8-cineole is induced in the proliferating liquid and is handled, method according to common tissue cultivating seedling is cultivated, can take root in 7-10 days, can transplant nutrient cup or seedbed cultivation in 40-60 days.
Outstanding substantive distinguishing features of the present invention and obvious improvement are:
1, through the practice of cuttage and seedling culture in a few years, the applicant found out a cover fast, high-quality, the cycle is short, survival rate is high cuttage raise seedling technique.Take 1,8-cineole bud is induced to handle in the proliferating liquid eucalyptus type narrow leaved tea tree plant variety is compared and screens, through the cultivation in some generations.The cineole of finding indivedual individual plants improves gradually, and by more than 50% the original beginning, a generation generation improves, and is stabilized in more than 70%.And tree-like sturdy, tree crown is bigger, is thick with leaves, and is short than other narrow leaved tea tree kind.Take steam distillation to put forward yield up to 1.4%-1.8%, than other belong to together kind to put forward yield high about one times.And successfully make its plantation begin to enter the implementation phase, these new varieties will make and be difficult at present obtain 1, and the situation of 8-cineole no longer takes place, and for extracting 1, the chemistry of forest product industry of 8-cineole will bring once new leap.
2, adopt two kinds of tissue culture and rapid propagation methods of the present invention can both guarantee the fine quality of tissue cultivating seedling, tissue cultivating seedling or cuttage seeding are transplanted the land for growing field crops and are detected after 3 years, sapling keeps crown good, the fast growth of former fine individual plant, the primary characteristic that biomass is high (every strain can be adopted branches and leaves 3.5-5.5 kilogram every year).1, the content of 8-cineole and fine individual plant basically identical, 1, the 8-cineole is carried the yield height.More than two kinds of tissue culture and rapid propagation methods be that eucalyptus type narrow leaved tea tree improved seeds large tracts of land is promoted and laid a solid foundation.
3, the present invention is because the cuttage and seedling culture process has adopted elder generation that branch is soaked with organic solvent, make the higher wound of oil-containing to deoil, the better bud that absorbs is induced propagation solution, improved the survival rate of cuttage seeding, the highest can reaching more than 98% overcome the difficult problem that present narrow leaved tea tree sapling is difficult to survive.
4, cultivating the eucalyptus type narrow leaved tea tree plant variety that obtains with the present invention grows rapidly, tissue cultivating seedling transplants that can to grow 2-3 rice in back 1 year high, can gather in top branch (above 20 centimetres of root) steam oil, the branch that the harvesting back root part grows is very easy to grow new twig, as long as keep moisture and fertilizer, can gather in the branches and leaves steam oil again after 4-6 month, can gather in every year more than 2 times, along with the time lengthening of plantation, output keeps stable high yield in 10 years continuously along with raising, so just alleviated 1 of the present China and the world, the situation of 8-cineole anxiety, particularly China Guangxi, Guangdong, Hainan, Fujian, the mountain area, rural area in Yunnan is if these new varieties are come into poor mountain area, be about to become new projects of peasant programme, simultaneously, by fresh branch and leaf are extracted 1, behind the 8-cineole, deep processing can pharmacy, branch can also be used for papermaking, and beaver board and fire charcoal etc. is for a kind of better outlet has been found in the comprehensive exploitation of narrow leaved tea tree.
5, new varieties of the present invention detect through provincial examination and test of products department, and its chemical composition content is 1,8-cineole: 70～82%; 4-terpineol: 1～1.2%; A-terpineol: 7～9%; Optical activity :+2～3 °; Refraction index (20 ℃): 1.4630.Color is transparent.
Embodiment:
Embodiment 1
Preferred eucalyptus type narrow leaved tea tree seeds, leaf is shaped as needle-like, analyze the chemical composition of seeds earlier, regather 1, the seed that 8-cineole content is high, generally choose 1, the kind of 8-cineole more than 50% screened, adopt ultraviolet illumination, with disinfectant seed soaking 10-30 minute, will damage then, full or not different in nature seed is removed, and cultivates in booth, select tree-like sturdy, tree crown is bigger, the be thick with leaves eucalyptus type narrow leaved tea tree seeds of fast growth are adopted the branch cutting method, are containing 1, the bud of 8-cineole is induced in the proliferating liquid and is handled, seed selection, preferred through several generations, obtain 1 of individual plant, 8-cineole content up to the improved seeds more than 70% as female parent, choose the annual semi-lignified of fine individual plant after the plantation, healthy, disease-free branch adopts cuttage or tissue culture method to cultivate, and obtains sprigging to big Tanaka.The method of cuttage is to select sturdy, the semi-lignified tender sprout of annual sapling (to grow up to behind tissue cultivating seedling or the cuttage seeding transplanting land for growing field crops), about length 13-15 centimetre, earlier soak branch Lower Half (cuttage bury part) 3-5 hour (because of the shoot oil content height of eucalyptus type narrow leaved tea tree with the 3-8% propyl alcohol, the purpose of soaking is de-oiling, in order to taking root).Then sterilized 1-5 minute with the potassium permanganate of 0.1-1.0%; Then, placement prepares in advance, contains 1, and the bud of 8-cineole is induced in the proliferating liquid and handled, and gets final product cuttage.Will be before the cuttage with the nutritive mud in the nutritious bag, 7-10 days in advance is formalin (formaldehyde) sterilization of 1-5% with concentration, and the cuttage degree of depth is long 1/3 being advisable of branch, and branch is moistening gets final product in maintenance after the cuttage.The cuttage of eucalyptus type narrow leaved tea tree is in planting percent the best in August to December in every year.Took root in general 20 days, 2 wheat harvesting periods can be planted, and emergence rate is more than 90%.Cuttage seeding survives transplanting land for growing field crops, back and detects after 3 years, keeps the fine quality of former fine individual plant equally.
Embodiment 2
Preferred eucalyptus type narrow leaved tea tree seeds, in booth, cultivate, select tree-like sturdy, tree crown is bigger, be thick with leaves, the eucalyptus type narrow leaved tea tree seeds of fast growth adopt the branch cutting method, containing 1, the bud of 8-cineole is induced in the proliferating liquid and is handled, seed selection again, preferred through several generations, obtain 1 of individual plant, 8-cineole content as female parent, is chosen the annual semi-lignified of fine individual plant, health, disease-free branch up to the improved seeds more than 70% after the plantation, adopt cuttage or tissue culture method to cultivate, obtain sprigging to big Tanaka.The method of cuttage is to select sturdy, the semi-lignified tender sprout of annual sapling, about length 13-15 centimetre, earlier soak branch Lower Half (cuttage bury part) 3-5 hour (because of the shoot oil content height of eucalyptus type narrow leaved tea tree with the 1-5% lauryl sodium sulfate, the purpose of soaking is de-oiling, in order to taking root).Then sterilized 1-5 minute with the potassium permanganate of 0.1-1.0%; Then, what placement prepared in advance contains 1, and the bud of 8-cineole is induced in the proliferating liquid and handled, and gets final product cuttage.Will be before the cuttage with the nutritive mud in the nutritious bag, 7-10 days in advance is formalin (formaldehyde) sterilization of 1-5% with concentration, and the cuttage degree of depth is long 1/3 being advisable of branch, and branch is moistening gets final product in maintenance after the cuttage.The cuttage of eucalyptus type narrow leaved tea tree is in planting percent the best in August to December in every year.Took root in general 20 days, 2 wheat harvesting periods can be planted, and emergence rate is more than 90%.Cuttage seeding survives transplanting land for growing field crops, back and detects after 3 years, keeps the fine quality of former fine individual plant equally.The method of group training is, with preferred narrow leaved tea tree leaf nose part, through disinfecting (sterilization method is identical with the sterilization method of common plant tissue culture seedling), what adding prepared in advance then contains 1, the bud of 8-cineole is induced in the proliferating liquid and is handled, method according to common tissue cultivating seedling is cultivated, and can take root in 7-10 days, can transplant nutrient cup or seedbed cultivation in 40-60 days.
Embodiment 3
Preferred eucalyptus type narrow leaved tea tree seeds, leaf is shaped as needle-like, analyze the chemical composition of seeds earlier, regather the seed that contains the folium eucalypti innage, generally choose 1, the kind of 8-cineole more than 50%, screen, adopt ultraviolet illumination, then with disinfectant seed soaking 10-30 minute, to damage, full or not different in nature seed is removed, in booth, cultivate, select tree-like sturdy, tree crown is bigger, the eucalyptus type narrow leaved tea tree seeds of fast growth of being thick with leaves are adopted the branch cutting method, containing 1, the bud of 8-cineole is induced in the proliferating liquid and is handled, extract 1,8-cineole assay, seed selection again, preferred through several generations, obtain 1 of individual plant, as female parent, the sturdy of annual sapling (to grow up to behind the tissue cultivating seedling transplanting land for growing field crops) selected in the plantation back to 8-cineole content up to the improved seeds more than 70%, the semi-lignified tender sprout, about length 13-15 centimetre, earlier soak branch Lower Half (cuttage bury part) 3-5 hour (because of the shoot oil content height of eucalyptus type narrow leaved tea tree, the purpose of immersion is de-oiling, in order to taking root) with 3-10% ethanol (can use the 70-95% ethanol dilution).Then sterilize with the potassium permanganate of 0.1-1%; Then, place the bud prepare in advance and induce the propagation medicament of taking root to handle, get final product cuttage.Will be before the cuttage with the nutritive mud in the nutritious bag, 7-10 days in advance is formalin (formaldehyde) sterilization of 1-8% with concentration, and the cuttage degree of depth is long 1/3 being advisable of branch, and branch is moistening gets final product in maintenance after the cuttage.The cuttage of eucalyptus type narrow leaved tea tree is in planting percent the best in August to December in every year.Took root in general 20 days, 2 wheat harvesting periods can be planted, and emergence rate is more than 90%.Cuttage seeding survives transplanting land for growing field crops, back and detects after 3 years, keeps the fine quality of former fine individual plant equally.Obtain after the improved seeds, adopt the method for cuttage or group training to cultivate, method is identical with the method for embodiment 2.
Embodiment 4
Contain 1, the bud of 8-cineole is induced the preparation (1) of proliferating liquid: with the mixed solution of indole-3-butyric acid (IBA)+0.1-0.5mg/L methyl (NAA) of 0.1-0.5mg/L, add the ABT1 root-inducing powder of 0.1-0.5mg/L, also add agar 3-10g/L in the medium, sucrose 10-40g/L, 1,8-cineole 1-5g/L regulates pH5.5-6.5, adds distilled water, reach 1 liter, can use.
Embodiment 5
Contain 1, the bud of 8-cineole is induced the preparation of proliferating liquid (2): with 2,4 dichlorophenoxyacetic acid (2,4-D) the mixed solution of 0.1-0.5mg/L, indole-3-butyric acid (IBA) 0.1-0.5mg/L 0.1-0.5mg/L methyl (NAA), add the ABT5 root-inducing powder of 0.1-0.5mg/L, also add agar 3-10g/L in the medium, sucrose 10-40g/L, 1,8-cineole 1-5g/L regulates pH5.5-6.5, adds distilled water, reach 1 liter, can use.
Embodiment 6
Contain 1, the bud of 8-cineole is induced the preparation (3) of proliferating liquid: with the mixed solution of TDZ0.1-0.5mg/L, indole-3-butyric acid (IBA) 0.1-0.5mg/L 0.1-0.5mg/L methyl (NAA), add the ABT8 root-inducing powder of 0.1-0.5mg/L, also add starch 3-10g/L in the medium, glucose 10-40g/L, 1,8-cineole 1-5g/L regulates pH5.5-6.5, adds distilled water, reach 1 liter, can use.Regulate pH5.5-6.5, add distilled water, reach 1 liter, can use.
Embodiment 7
Contain 1, the bud of 8-cineole is induced the preparation (4) of proliferating liquid: with the mixed solution of 0.1-0.5mg/L MS medium, 0.1-0.5mg/L benzyl purine (BA), 0.1-0.5mg/L methyl (NAA), add the ABT9 root-inducing powder of 0.1-0.5mg/L, also add starch 3-10g/L in the medium, glucose 10-40g/L, 1,8-cineole 1-5g/L regulates pH5.5-6.5, adds distilled water, reach 1 liter, can use.Regulate pH5.5-6.5, add distilled water, reach 1 liter, can use.
Embodiment 8
Contain 1, the bud of 8-cineole is induced the preparation (5) of proliferating liquid: with the mixed solution of 0.1-0.5mg/L MS medium, 0.1-0.5mg/L indole-3-butyric acid (IBA), 0.5-1.0mg/L methyl (NAA), add the ABT5 root-inducing powder of 0.1-0.5mg/L, also add agar 3-10g/L in the medium, sucrose 10-40g/L, 1,8-cineole 1-5g/L regulates pH5.5-6.5, adds distilled water, reach 1 liter, can use.
Embodiment 9
Contain 1, the bud of 8-cineole is induced the mixed solution of the preparation (6) of proliferating liquid with 0.1-0.8mg/L N5,0.1-0.8mg/L indole-3-butyric acid (IBA), 0.1-0.8mg/L benzyl purine (BA), add the ABT9 root-inducing powder of 0.1-0.5mg/L, also add agar 3-10g/L in the medium, sucrose 10-40g/L, 1,8-cineole 1-5g/L regulates pH5.5-6.5, adds distilled water, reach 1 liter, can use.
Embodiment 10
Contain 1, the bud of 8-cineole is induced the mixed solution of the preparation (7) of proliferating liquid with 1/2MS medium, 0.1-0.6mg/L indole-3-butyric acid (IBA), add the ABT8 root-inducing powder of 1.0-2.0mg/L, also add starch 3-10g/L in the medium, glucose 10-40g/L, 1,8-cineole 1-5g/L regulates pH5.5-6.5, adds distilled water, reach 1 liter, can use.Regulate pH5.5-6.5, add distilled water, reach 1 liter, can use.
Eucalyptus type narrow leaved tea tree of the present invention has developed into scale more than 500 mu at present through breeding and cultivating, and variety and quality is stable, has reached the primary condition of new variety of plant.
Below be the testing result of eucalyptus type narrow leaved tea tree of the present invention:
Guangxi * * provincial forest-science detects institute and detects 1, the content results of 8-cineole:
Certain base, the continuous district of Yulin City of Guangxi Zhuang Autonomous good fortune: 26 days 1 March in 2006, the content 75.81% of 8-cineole;
Yulin City of Guangxi Zhuang Autonomous Yuzhou District base: 24 days 1 April in 2006, the content 80.11% of 8-cineole;
Base, Beiliu City, Guangxi: 19 days 1 May in 2006, the content 74.61% of 8-cineole;
Base, Xingye County, Guangxi: 11 days 1 December in 2006, the content 71.75% of 8-cineole.
Claims (4)
1, a kind of cultivation of eucalyptus type narrow leaved tea tree and numerous method for planting, it is characterized in that: in booth, cultivate, select tree-like sturdy, tree crown is bigger, the tree type that is thick with leaves analyzes 1,8-cineole content, preferred through several generations, obtain 1 of individual plant, 8-cineole content as female parent, adopts cottage method or tissue culture method to cultivate and breed up to the improved seeds more than 70% then;
The bud that adopts in described cottage method or the tissue culture method induces proliferating liquid to contain 1, the 8-cineole.
2, the cultivation of eucalyptus type narrow leaved tea tree according to claim 1 and numerous method for planting, it is characterized in that: 1 of described individual plant, 8-cineole content up to the selection of the improved seeds more than 70% is: preferred eucalyptus type narrow leaved tea tree seeds, analyze the chemical composition of seeds earlier, regather 1, the 8-cineole contains high seed, screen, adopt ultraviolet illumination, then with disinfectant seed soaking 10-30 minute, to damage, full or not different in nature seed is removed, and cultivates in booth, selects tree-like sturdy, tree crown is bigger, the eucalyptus type narrow leaved tea tree seeds of fast growth of being thick with leaves are adopted the branch cutting method, containing 1, the bud of 8-cineole is induced in the proliferating liquid and is handled, again seed selection.
3, the cultivation of eucalyptus type narrow leaved tea tree according to claim 1 and numerous method for planting, it is characterized in that: described cottage method is: the tender sprout of sturdy, semi-lignified of selecting the sapling of life in 1 year, about length 13-15 centimetre, soak branch cutting with organic solvent earlier and buried part 3-5 hour, then sterilized 1-5 minute with the potassium permanganate of 0.1-1.0%; What placement prepared in advance then contains 1, and the bud of 8-cineole is induced in the proliferating liquid and handled, and gets final product cuttage;
Described organic solvent is selected in propyl alcohol, isopropyl alcohol, ether or the ethylene glycol at lauryl sodium sulfate, aliphatic alcohol sulfate, methyl alcohol, ethanol, and mass concentration is 1-10%.
4, the cultivation of eucalyptus type narrow leaved tea tree according to claim 1 and numerous method for planting, it is characterized in that: described tissue culture method is: with preferred narrow leaved tea tree leaf nose part, through disinfecting, what adding prepared in advance then contains 1, the bud of 8-cineole is induced in the proliferating liquid and is handled, and cultivates according to the method for common tissue cultivating seedling.
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| CN 200710027978 CN101049074A (en) | 2007-05-14 | 2007-05-14 | Breeding and reproducing eucalyptus type cajeput with alternate leaves |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102342232A (en) * | 2010-08-04 | 2012-02-08 | 湖南康普医药研究院 | Eucalyptus type melaleuca alternifolia seedling raising and planting method |
| CN102577942A (en) * | 2011-01-06 | 2012-07-18 | 厦门涌泉集团有限公司 | Tissue culture method of callistemon hybridus 'golden ball' |
| CN102870675A (en) * | 2012-09-26 | 2013-01-16 | 李斌 | Method for tissue culture of sprout |
| CN106416680A (en) * | 2016-09-08 | 2017-02-22 | 广西壮族自治区林业科学研究院 | Melaleuca alternifolia cutting propagation method |
-
2007
- 2007-05-14 CN CN 200710027978 patent/CN101049074A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102342232A (en) * | 2010-08-04 | 2012-02-08 | 湖南康普医药研究院 | Eucalyptus type melaleuca alternifolia seedling raising and planting method |
| CN102577942A (en) * | 2011-01-06 | 2012-07-18 | 厦门涌泉集团有限公司 | Tissue culture method of callistemon hybridus 'golden ball' |
| CN102870675A (en) * | 2012-09-26 | 2013-01-16 | 李斌 | Method for tissue culture of sprout |
| CN106416680A (en) * | 2016-09-08 | 2017-02-22 | 广西壮族自治区林业科学研究院 | Melaleuca alternifolia cutting propagation method |
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