CN101019964A - Health wine with leech and its production process - Google Patents

Abstract

The present invention discloses one kind of health wine with leech and its production process. The health wine is prepared with leech, wolfberry fruit, ginkgo leaf extract, honey and liquor in certain weight proportion, and through traditional soaking process. It has the main active component of leech, natural hirudin, preserved, and its hirudin content may be detected through direct thrombin titration. The health wine can improve blood circulation, promote metabolism, dilate capillary vessel and result in other health functions to prevent and treat diseases and solve subhealth problem.

Description

A kind of health wine with leech and production method thereof

Technical field

The present invention relates to the healthy medicated wine of a kind of alcopop, particularly hirudin.

Background technology

Along with Chinese society and economy develop fast, science and technology is constantly progressive, living standards of the people improve day by day, the variation of essence has taken place in dietary structure, the nutrient substance such as fat, albumen and saccharide of many people's excess intakes in daily diet, substantially exceeded the nutritional need of self, material more than needed is just piled up in vivo everywhere, blood vessel blockage, organ hypertrophy or phenomenons such as dysfunction, oils and fats lamination occur, form obesity, cardiovascular and cerebrovascular disease, diabetes etc. modern so-called " affluenza " thus; The life stress that adds the modern increases, and the routine work life does not often have rule, also has the combined influence of some bad dietary habit as the excessive drinking of smoking etc., and the crowd who is in " subhealth state " state is more and more huger.And the root of above-mentioned two kinds of symptoms all is the mobile variation of blood, and blood is not smooth, and every metabolism of human body just can not normally carry out, and various diseases and defective mode are just numerous and confused to be occurred.

Summary of the invention

The object of the present invention is to provide a kind of health wine with leech, it has the blood circulation of improvement, enhances metabolism, effects such as expansion blood capillary, blood viscosity lowering, removing free radical, thus can prevent and treat affluenza, solve the problem of subhealth state.

Another object of the present invention provides a kind of production method of described health wine with leech.

Technical scheme of the present invention is achieved in that

The prescription that product of the present invention adopted comprises the bright product of Hirudo, Fructus Lycii, Folium Ginkgo extract, Mel and wine, and the weight part ratio of each component is as follows:

1～5 part of Hirudo, 1～4 part of Fructus Lycii, 1～4 part of Folium Ginkgo extract, 10～100 parts of wine, 1～4 part of Mel, 0～100 part of pure water.

In the above-mentioned prescription, Hirudo comprises aquatic foods product or the dry product of the class Hirudos of sucking blood such as Hirudinaria manillensis, blood-eating hirudo, excellent stricture of vagina cattle trematodiasis, haemadipsa japonica and Haemadipsa hainana, preferred bright product; Folium Ginkgo extract is a water-soluble products, and used wine is that rice wine or Sorghum vulgare Pers. Wine spirituosity degree are more than 40 degree.

Hirudo should be done following processing earlier before use: with fresh goods clean water wash clean, quick-freezing is taken out airing water after it is caused death, and is standby.Dry product can directly soak.

Product of the present invention adopts its quality of following control indexes:

Hirudin 1～50ATU/ml, Ginkgo total flavones 0.2～0.4mg/L.

The preparation method of product of the present invention is:

1, Fructus Lycii, Hirudo are placed in the lump the above wine of 40 degree that has just soaked last Fructus Lycii and Hirudo, sealing was soaked 1～4 month, soaked the after-filtration that finishes, and got filtrate, and supernatant is got in clarification, and is standby;

2, wine, Mel and the pure water of getting Folium Ginkgo extract, surplus in proportion places container to mix with above-mentioned supernatant in the lump, and supernatant is got in clarification, promptly obtains final products of the present invention.

The invention has the advantages that the quality that the content that can utilize Markward t thrombin direct titrimetric method to measure hirudin comes monitoring product.Its principle is: the main active of Hirudo is a hirudin, and hirudin can combine with the thrombin specificity, make the thrombin inactivation, it is 1: 1 in conjunction with ratio, the measurement unit of hirudin is an iu, represent that with ATU the iu of thrombin activity is NIH, during promptly 1 ATU equals and the hirudin amount of 1 NI H thrombin.

Hirudin detection method of content in the product of the present invention:

1.1 reagent and material

The Tris-HCl buffer (pH7.54) of 0.9% normal saline, 0.2mol/L, fibrinogen (biochemical reagents, thrombin (biochemical reagents), stopwatch, whiteware titer plate, microsyringe (1-500 μ L).

1.2 the preparation of solution

1.2.1 the preparation Tris hydrochloride buffer of Tris-HCl buffer (pH7.54) (is got 0.2mol/L tris solution 25ml and the about 40ml of 0.1mol/L hydrochloric acid solution, add water to 100ml, regulate pH value to 7.4) (matching while using).

1.2.2 the preparation of 0.5% fibrinogen: with 4.2 preparations.

1.2.3 the preparation of thrombin solution: the solution that is mixed with the variable concentrations gradient with 0.9% normal saline, be that (be benchmark concentration, promptly 1 times, its 5 μ L is a titration volume to 20NIH/ml, be 1V, be 0.1ATU), 40NIH/ml (promptly 2 times), 100 NIH/ml (promptly 5 times), and the like.(matching while using)

1.3 operational approach

Get several 0.75 * 10cm small test tubes, each adds 0.5% fibrin original solution, 200 μ L, respectively add testing sample 50Ml again, be placed on 5min in 37 ℃ of thermostat water baths, getting one props up, Dropwise 5 thrombin liquid 5 μ L doubly shake up rapidly, if solidify (observing) in room temperature in 1 minute on the whiteware titer plate; If do not solidify in the room temperature 1min, then Dropwise 5 times thrombin 5 μ L again condense up to fibrinogen, write down used thrombin solution cumulative volume.

1.3.1 blank test: get 0.75 * 10cm small test tube and add 0.5% fibrinogen solution, 200 μ l, add normal saline 50 μ l again as blank, shake up, with blowing minute bubbles in the pipettor fibrinogen solution in vitro, as observing labelling.Add 100NI H/ml thrombin solution 5 μ l and shook up for 6 seconds gently, (attention avoids producing other bubble), then at room temperature (about 25 ℃) with rotating test tube in the test tube rack gently, when reaction was less than terminal point, minute bubbles can rest on the top of reactant liquor always; When reaction was reached home, minute bubbles rotated and rotate along with test tube, were reaction end this moment.The blank response time should be controlled at (± 5 seconds) about 1 minute.As not in this scope, then fibrinogen concentration should be regulated or thrombin remeasures.

1.3.2 hirudin detection method of content in the product of the present invention: operation is identical with the blank test.The normal saline that just replaces blank with product solution of the present invention to be measured 50 μ l.When response time to 1 minute, still rest on the top of reactant liquor as minute bubbles, promptly fibrin does not condense, and continues this moment to add 100NIH/ml thrombin solution 5 μ l, continues to observe.Added thrombin 1 time every 1 minute, till fibrinogen solution condenses.(if it is still noncondensing more than 3 times to add thrombin, then needs the Hirudo sample is diluted detection again).Be when adding blood coagulation, enzyme from the first time, to add 1 thrombin until reaction end every 1 minute computation time.

4.4 the result calculates:

1ml product solution hirudin of the present invention active unit=

Each amount * (enzyme-added number of times-blank 1 time)/0.05ml (sample size) that adds thrombin

Hirudin anticoagulant hemase vigor calculates by (A-1) in the sample liquid.

X1＝V×20………………………… (A-1)

Wherein:

X1---hirudin anticoagulant hemase vigor, ATU/ml;

V---consumption thrombin solution cumulative volume, ml.

Described below is the relevant effect of above-mentioned key component:

Hirudo: contain abundant natural hirudin in the salivary gland of Hirudo and the secreted saliva thereof, the low molecular polypeptide that it is made up of 65～66 aminoacid, molecular weight are 7000, very easily water-soluble, highly stable under the room temperature, be heated to 80 ℃, 40min is not destroyed.

The strongest natural antithrombase material in the material that natural hirudin is up to now to be found has specially good effect to thrombotic disease such as human cardiovascular and cerebrovascular diseases especially cerebral thrombosis, its prevention effect be any material can not compare.Scientific research simultaneously proves that also natural hirudin is the material base of Hirudo performance drug effect.In addition, natural hirudin can also play and improve blood circulation, enhances metabolism, blood viscosity lowering, maintenance blood vessel elasticity, makes the unobstructed effect of blood flow.

Folium Ginkgo extract: contain flavone compound and distinctive Ginkgo total lactones two class bioactive substances, can remove the rubbish in the blood, improve blood circulation, strengthen brain vigor and neural health, improving memory delays the brain cell aging, go back the scalable blood fat simultaneously, remove free radical, improve moisture content of skin and fat content, suppress the generation of wrinkle.

Wine: wine energy blood circulation promoting has effects such as blood-enriching face-nourishing, relaxing muscles and tendons and activating QI and blood in the collateral, building body and life lengthening.

Mel: Mel delicate fragrance is sweet, and nutritious tonifying has heat clearing away, invigorating middle warmer, detoxifies, moisturizes, effect such as convergence.The Nulomoline more than 70% that contains in the Mel can directly be absorbed by human body intestinal wall cell without digestion.Mel is not fatty, therefore is equally applicable to obesity, old age, hypertension blood fat crowd;

Pure water: do dilution and use.

Product of the present invention, the medicated wine combination, efficacy enhanced by wine, wine helps the efficacy of a drug, make natural hirudin and flavone compound and Ginkgo total lactones two class bioactive substances in human body, play rapidly to improve blood circulation, enhance metabolism, effects such as expansion blood capillary, blood viscosity lowering, removing free radical, thus above-mentioned affluenza can be prevented and treated, solve the problem of subhealth state.

The specific embodiment

Embodiment one

1, takes by weighing the bright product 1Kg of Hirudinaria manillensis, the Fructus Lycii 1Kg of airing respectively, place in the lump in the cylinder, put into the rice wine 10Kg sealing of 52 degree then and soaked 1 month.1 month after-filtration is got filtrate, and clarification is 3 days naturally, gets supernatant 7.8Kg, and is standby;

2, take by weighing Folium Ginkgo extract 1Kg, Mel 1Kg respectively, place in the lump in the cylinder, pour the rice wine 10Kg and the above-mentioned supernatant 7.8Kg of 52 degree then respectively into, mix, clarification is 3 days naturally, gets supernatant 19.4Kg, promptly becomes final products of the present invention.After testing, the alcoholic strength of this product is that the content of 42.3 degree, hirudin is 10ATU/ml, and Ginkgo total flavones content is 0.3mg/L.

Embodiment two

1, takes by weighing blood-eating hirudo dry product 5Kg, Fructus Lycii 4Kg respectively, place in the lump in the cylinder, put into the rice wine 80Kg sealing of 50 degree then and soaked 2 months.2 months after-filtration are got filtrate, and clarification is 2 days naturally, gets supernatant 70.3Kg, and is standby;

2, take by weighing Folium Ginkgo extract 4Kg, Mel 4Kg, pure water 100Kg respectively, place in the lump in the cylinder, pour the rice wine 20Kg and the above-mentioned supernatant 70.3Kg of 50 degree then respectively into, mix, naturally clarification is 3 days, gets supernatant 193.2Kg, promptly becomes final products of the present invention.After testing, the alcoholic strength of this product is that the content of 26.5 degree, hirudin is 5ATU/ml, and Semen Ginkgo General Political Department flavones content is 0.4mg/L.

Embodiment three

1, take by weighing Hirudinaria manillensis dry product 3Kg respectively, Fructus Lycii 1Kg places in the cylinder in the lump, puts into the rice wine 20Kg sealing of 55 degree then and soaks 3 months.3 months after-filtration are got filtrate, and clarification is 3 days naturally, gets supernatant 14.6Kg, and is standby;

2, take by weighing Folium Ginkgo extract 2Kg, Mel 3Kg, pure water 30Kg respectively, place in the lump in the cylinder, pour the rice wine 15Kg and the above-mentioned supernatant 14.6Kg of 55 degree then respectively into, mix, naturally clarification is 2 days, gets supernatant 45.8Kg, promptly becomes final products of the present invention.After testing, the alcoholic strength of this product is that the content of 23.4 degree, hirudin is 20ATU/ml, and flavonoid of ginkgo biloba is 0.2mg/L.

Claims (5)

1, a kind of health wine with leech is characterized in that it is made by the following raw material of routine weight part ratio:

1～5 part of Hirudo, 1～4 part of Fructus Lycii, 1～4 part of Folium Ginkgo extract, 100 parts of wine, 1～4 part of Mel, 0～100 part of pure water.

2, healthy medicated wine according to claim 1 is characterized in that this production method comprises the following steps:

A, Hirudo, Fructus Lycii are placed in the lump an amount of wine seal in proportion to soak 1～4 month, and soaked the after-filtration that finishes, get filtrate, supernatant is got in clarification, and is standby;

B, wine, Mel and the pure water with Folium Ginkgo extract, surplus places container to mix with above-mentioned supernatant in the lump in proportion, and supernatant is got in clarification, promptly obtains final products of the present invention.

3, healthy medicated wine according to claim 1 and 2 is characterized in that:

Described Hirudo comprises aquatic foods product or the dry product of Hirudinaria manillensis, blood-eating hirudo, excellent stricture of vagina cattle trematodiasis, haemadipsa japonica and Haemadipsa hainana and the class Hirudo of sucking blood thereof, preferred bright product.

4, healthy medicated wine according to claim 1 and 2 is characterized in that:

Folium Ginkgo extract is a water-soluble products, and used wine is rice wine or Sorghum vulgare Pers. Wine, and alcoholic strength is more than 40 degree.

5, healthy medicated wine according to claim 1 and 2 is characterized in that:

The hirudin content of this healthy medicated wine is 1～50ATU/ml, and Ginkgo total flavones content is 0.2～0.4mg/L.