CN101008649A - Multi-probe optical fiber evanescent wave biological detector based on correlation detection - Google Patents
Multi-probe optical fiber evanescent wave biological detector based on correlation detection Download PDFInfo
- Publication number
- CN101008649A CN101008649A CN 200710036880 CN200710036880A CN101008649A CN 101008649 A CN101008649 A CN 101008649A CN 200710036880 CN200710036880 CN 200710036880 CN 200710036880 A CN200710036880 A CN 200710036880A CN 101008649 A CN101008649 A CN 101008649A
- Authority
- CN
- China
- Prior art keywords
- circuit
- temperature
- sample cell
- sample
- signal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 title claims abstract description 61
- 238000001514 detection method Methods 0.000 title claims abstract description 29
- 239000013307 optical fiber Substances 0.000 title abstract description 17
- 238000012545 processing Methods 0.000 claims abstract description 25
- 230000003287 optical effect Effects 0.000 claims abstract description 21
- 230000002093 peripheral effect Effects 0.000 claims abstract description 9
- 238000010438 heat treatment Methods 0.000 claims description 17
- 239000000835 fiber Substances 0.000 claims description 16
- 230000005284 excitation Effects 0.000 claims description 11
- 230000010363 phase shift Effects 0.000 claims description 11
- 230000001427 coherent effect Effects 0.000 claims description 9
- 239000013068 control sample Substances 0.000 claims description 3
- 230000035945 sensitivity Effects 0.000 abstract description 8
- 230000010354 integration Effects 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 description 15
- 239000007850 fluorescent dye Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000006870 function Effects 0.000 description 4
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 239000004065 semiconductor Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 3
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 238000013480 data collection Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000004313 glare Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000007639 printing Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000218691 Cupressaceae Species 0.000 description 1
- 206010035148 Plague Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 206010064097 avian influenza Diseases 0.000 description 1
- 238000009529 body temperature measurement Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000005055 memory storage Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 230000003534 oscillatory effect Effects 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000005676 thermoelectric effect Effects 0.000 description 1
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
A multi-probe optical fiber evanescent wave biological detector based on correlation detection comprises an optical system, a photoelectric detector, a sample flow path, a data acquisition and signal processing system, a one-dimensional scanning platform, a stepping motor and a plurality of optical fibers, and is characterized by further comprising: the related detection circuit and the temperature control system for controlling the temperature of the sample pool, wherein the data acquisition and signal processing system consists of an embedded microprocessor chip and a peripheral control part thereof. The invention further reduces the system noise, improves the detection efficiency and the sensitivity of the system, and has the characteristic of small integration.
Description
Technical field
The present invention relates to the fast travelling waves of optical fibre Measurement for Biotechnique, particularly a kind of many probes optical fibre bio detector based on coherent detection is the improvement to many probes optical fibre bio detector.This detector can be surveyed a various biomolecules in the sample simultaneously, as virus, bacterium, noxious material etc., and its dynamic process is monitored, and can satisfy some special occasions needs of the multiple biological substance of fast detecting simultaneously.This detector can widespread use in biomedical research, Food Inspection, environmental monitoring, biological warfare agent are surveyed.
Background technology
The evanescent wave that multiprobe fiber optical evanescent wave biotester produces when utilizing light wave to transmit in the total reflection mode in optical fiber comes the fluorescent dye of excitation fiber fibre core surface indicia on biomolecule to be measured, be attached to the attribute of biological substance in the scope of evanescent wave field, fibre core surface and the detection of content thereof by specific reaction, realize identification and detection multiple biological substance.
Formerly the structure of a multiprobe fiber optical evanescent wave biotester in the technology as shown in Figure 1, this detector mainly is made up of optical system 1, photodetector 2, sample flow path 3, data acquisition and signal processing system 4, one-dimensional scanning platform 5, stepper motor 6 and optical fiber 10.Wherein, optical system 1 comprises laser instrument 101, excitation light path 102 and fluorescence receiving light path 103.Its course of work is as follows: in the sample cell solution mark fluorescent dye biomolecule to be measured be fixed in the biological identification molecule generation specific reaction of optical fiber surface after, fluorescent dye is fixed in the fibre core surface thereupon; The multifiber 10 that the optical system of the laser that laser instrument 101 sends by detector is opposite in the sample cell 303 under the room temperature scans detection, the fluorescent dye Stimulated Light of optical fiber surface excites the back to produce faint fluorescence signal, through being projeced into behind the fluorescence receiving light path 103 on the photodetector 2, the electric signal of photodetector 2 outputs is made data collection and signal Processing by general-purpose computing system.
The shortcoming of above-mentioned technology formerly is:
1, the signal to noise ratio (S/N ratio) of system is lower.Formerly in the technology, extraneous veiling glare, laser itself all can produce fluorescence signal and disturb, and form noise; The electrical noise that the electronic devices and components of data acquisition and control section are introduced is also very important.Above-mentioned noise is low-frequency component normally, does not formerly have in the technology to adopt the means that can reduce or eliminate these low-frequency noises, so both all make the signal to noise ratio (S/N ratio) of this system reduce greatly.
2, detection speed and sensitivity are all lower.Be the fast reaction speed and the raising extent of reaction, the specific reaction between biological identification molecule and the checking matter should carry out under the biological living temperature.For example, the optimum temperature of antigen and antibody mediated immunity reaction is 37 ℃, and the optimum temperature of nucleic acid hybridization reaction is 42 ℃.And formerly technology lacks temperature control to solution in the sample cell, and reaction is at room temperature carried out, thereby can reduce detection speed and sensitivity.
3, power consumption is big and volume is big.Owing to formerly adopted general-purpose computing system to carry out work such as data acquisition and signal Processing in the technology, it is higher that power consumption and cost all compare, and mouse and keyboard etc. are peripheral hardware, not portable, causes instrument can't realize that complete machine is integrated.
Summary of the invention
The objective of the invention is to improvement, a kind of multiprobe fiber optical evanescent wave biotester based on coherent detection is provided above-mentioned technology formerly.This instrument should further reduce system noise, improves the detection efficiency and the sensitivity of system, makes instrument small-sized integrated.
The improved main thought of the present invention is: utilize related detecting method, the direct current signal of system is become high frequency ac signal, obtain final signal after correlator is handled, thereby reduced system noise, improve the detection sensitivity of system.Simultaneously, the solution of sample cell is carried out temperature control, make solution temperature be in the temperature of reaction of biological living,, improved detection efficiency and sensitivity so reaction velocity is fast, the extent of reaction is high.
In addition, data acquisition and signal processing system then adopt embedded microprocessor chip DSP to replace original general-purpose computing system, not only reduced the power consumption of system, volume also reduces greatly, and replaced the formerly external keyboard and the mouse of technology with simple and easy keyboard, realized that complete machine is integrated.
Technical solution of the present invention is as follows:
A kind of multiprobe fiber optical evanescent wave biotester based on coherent detection, comprise optical system, photodetector, sample flow path, data acquisition and signal processing system, one-dimensional scanning platform, stepper motor and multifiber, described optical system is made up of laser instrument, excitation light path and fluorescence receiving light path; Described sample flow path is made up of sample inlet, sample cell, sample export; Described multifiber one dimension is arranged in the described sample cell equally spacedly, and this sample cell places by on the described stepper motor driven one-dimensional scanning platform, is characterized in also comprising:
One correlation detecting circuit, this correlation detecting circuit comprises that high-frequency signal produces circuit and related operation circuit: the output terminal that described high-frequency signal produces circuit links to each other with the control end of described laser instrument, described high-frequency signal produces another output terminal of circuit through phase-shift circuit, the square wave drive circuit connects the reference input of the multiplier circuit of described related operation circuit, the signal input part of this multiplier circuit connects the output terminal of described photodetector, and the output terminal of this multiplier circuit links to each other with the input end of signal processing system with described data acquisition through the integrator circuit of described related operation circuit;
One control sample pool temperature control system, form by feed circuit, heating element, temperature sensor and temperature feedback circuit, feed circuit are powered to described sample cell heating to heating element, the temperature of this sample cell is surveyed by temperature sensor, the temperature information of the sample cell that the temperature that this temperature sensor is surveyed obtains after temperature feedback circuit output and target temperature are compared is sent into described feed circuit again, the power supply of control heating element, its function is to make the solution in the sample cell constant in required temperature.
Described data acquisition and signal processing system are made up of embedded microprocessor chip and peripheral control section thereof, this periphery control section comprises A/D converter, stepper motor driver, LCDs, mini-printer, clock chip, USB chip and driving circuit thereof, keyboard, RS232 interface, impact damper, and described microprocessor chip links to each other with above-mentioned each peripheral control section respectively.
Compare with technology formerly, technique effect of the present invention is as follows:
1. the signal to noise ratio (S/N ratio) of system improves greatly.
The modulation signal that high-frequency signal generation circuit sends can be controlled the output of excitation source, and the light intensity that excitation source is sent becomes the square wave with certain frequency.The related operation circuit produces circuit square-wave signal that modulates and the reference signal of passing through phase-shift circuit and square wave drive circuit with high-frequency signal and carries out related operation at correlator, realizes the detection to faint fluorescence, thereby has improved the signal to noise ratio (S/N ratio) of system.
The present invention has introduced correlation detecting circuit to the detection of fluorescent signals, it to external world the low-frequency noise that produces of veiling glare and electronic devices and components have very strong inhibition ability, thereby improved the signal to noise ratio (S/N ratio) of system significantly.
2. detection efficiency is high and highly sensitive.
Apparatus of the present invention are provided with temperature control system to sample cell, make the solution in the sample cell can constant temperature temperature required to the biological respinse of biomolecule to be measured, thereby have accelerated reaction velocity, improved the extent of reaction, have improved detection efficiency and sensitivity.
3. power consumption and volume all reduce.
The present invention has adopted embedded microprocessor chip DSP, and not only processing speed is fast for this microprocessor, volume is little, and low in energy consumption.
4. complete machine is realized integrated.
The present invention has replaced external units such as external keyboard in the technology formerly and mouse can be embedded into simple and easy keyboard in the cabinet and display screen, helps the integrated of complete machine.
Description of drawings
Fig. 1 is the structural representation of technology multiprobe fiber optical evanescent wave biotester formerly.
Fig. 2 is the structural representation that the present invention is based on the multiprobe fiber optical evanescent wave biotester of coherent detection.
Fig. 3 is the structural representation of correlation detecting circuit of the present invention.
Fig. 4 is the structural representation block diagram of temperature control system of the present invention.
Fig. 5 is data acquisition of the present invention and signal processing system structural representation.
Embodiment
The invention will be further described below in conjunction with drawings and Examples.
See also Fig. 2 earlier, Fig. 2 is the structural representation that the present invention is based on the multiprobe fiber optical evanescent wave biotester of coherent detection.As seen from the figure, the present invention is based on the multiprobe fiber optical evanescent wave biotester of coherent detection, comprise optical system 1, photodetector 2, sample flow path 3, data acquisition and signal processing system 4, one-dimensional scanning platform 5, stepper motor 6 and multifiber 10, described optical system 1 is made up of laser instrument 101, excitation light path 102 and fluorescence receiving light path 103; Described sample flow path 3 is made up of sample inlet 301, sample cell 303, sample export 302; Described multifiber 10 one dimensions are arranged in the described sample cell 303 equally spacedly, and this sample cell 303 places on the one-dimensional scanning platform 5 that is driven by described stepper motor 6, it is characterized in that also comprising:
One correlation detecting circuit, this correlation detecting circuit comprises that high-frequency signal produces circuit 9 and related operation circuit 7: the output terminal that described high-frequency signal produces circuit 9 links to each other with the control end of described laser instrument 101, described high-frequency signal produces another output terminal of circuit 9 through phase-shift circuit 901, square wave drive circuit 902 connects the reference input of the multiplier circuit 701 of described related operation circuit 7, the signal input part of this multiplier circuit 701 connects the output terminal of described photodetector 2, and the output terminal of this multiplier circuit 701 links to each other with the input end of described data acquisition with signal processing system 4 through the integrator circuit 702 of described related operation circuit 7;
The temperature control system 8 of described sample cell 303 temperature of one control, form by feed circuit 801, heating element 802, temperature sensor 803 and temperature feedback circuit 804, feed circuit 801 give heating element 802 power supplies to described sample cell 303 heating, the temperature of this sample cell 303 is surveyed by temperature sensor 803, the temperature information of the sample cell 303 that the temperature that this temperature sensor 803 is surveyed obtains after temperature feedback circuit 804 outputs are compared with target temperature is sent into the heating of described feed circuit 801 control heating elements 802 again, with the temperature of control sample cell 303.
Described data acquisition and signal processing system 4 adopt embedded microprocessor chip 401 and peripheral control section thereof to form, this periphery control section comprises A/D converter 402, stepper motor driver 403, LCDs 404, mini-printer 405, clock chip 406, USB chip and driving circuit 407 thereof, keyboard 408, RS232 interface 409, impact damper 410, and described microprocessor chip 401 links to each other with above-mentioned each peripheral control section respectively.
The control that the output intensity of described laser instrument 101 is subjected to high-frequency signal to produce circuit 9, its excitation beam that sends is injected optical fiber 10 through behind the excitation light path 102.Described photodetector 2 is converted to electric signal with the fluorescence signal of fluorescence receiving light path 103 outputs.The output of photodetector 2 enters data acquisition and signal processing system 4 behind related operation circuit 7.
As shown in Figure 3, described correlation detecting circuit comprises that high-frequency signal produces the multiplier circuit 701 and the integrator circuit 702 of circuit 9, phase-shift circuit 901, square wave drive circuit 902, photoelectric detector 2, related operation circuit 7.
As shown in Figure 4, described temperature control system 8 comprises feed circuit 801, heating element 802, temperature sensor 803, temperature feedback circuit 804.
As shown in Figure 5, described data acquisition and signal processing system 4 comprise stepper motor driver 403, clock chip 406, LCDs 404, keyboard 408, USB chip and driving circuit 407 thereof, mini-printer 405, RS232 interface 409, the impact damper 410 of microprocessor chip 401, A/D converter 402, control one-dimensional scanning platform 5.
Compare with technology formerly, characteristics of the present invention are exactly:
Adopt correlation detecting circuit that fluorescence signal is detected, make sample cell 303 constant temperature required with temperature control system 8; What data acquisition and signal processing system 4 adopted is microprocessor chip 401 and corresponding peripheral hardware thereof.
High-frequency signal generation circuit 9 in the correlation detecting circuit produces the two-way high-frequency signal, one road signal is used for modulated laser 101, the exciting light that laser instrument 101 is sent is modulated into the pulsed light that frequency is f, exciting light is through injecting the optical fiber 10 in the sample cell 303 behind the excitation light path 102, the fluorescence that inspires is gone into photodetector 2 through fluorescence receiving light path 103 is laggard, is converted into electric signal; Come in addition from the signal of high-frequency signal generation circuit 9 then as with reference to signal r, change the phase place of output signal among the reference signal r through phase-shift circuit 901, make it adjustable in 360 degree, through square wave drive circuit 902 signal wave of phase-shift circuit 901 is become the square-wave signal that dutycycle is 1:1 again, multiplier circuit 701 and integrator circuit 702 that the output electric signal of this signal and photodetector 2 together enters in the related operation circuit 7 carry out related operation.Related operation circuit 7 is electronic circuits of realizing reference signal r and the computing of measured signal s related function, ability with very strong inhibition noise, it adopts the simple crosscorrelation reception technique, by multiplier circuit 701 and integrator circuit 702, to amplify with the synchronous signal of high frequency reference signal r among the fluorescence signal s to be measured and detect, greatly reduced equivalent noise bandwidth, thereby reduced the noise of system, this output signal enters the A/D converter 402 of data acquisition and signal processing system 4 again.
Described temperature control system 8 adds direct current by the cold junction and the hot junction of 801 pairs of heating elements 802 of feed circuit, thermoelectric effect according to this heating element 802 obtains heat in the hot junction, the solution of supplying with in the sample cell 303 improves its temperature, described temperature sensor 803 is given temperature feedback circuit 804 with it after recording sample cell 303 temperature, by comparing with target temperature, send again and feed circuit 801, realize sample cell 303 temperature controlling.
Described microprocessor chip 401 is as the core of whole data collection and signal processing system 4, main by receiving order from keyboard 408, command each external unit to carry out function corresponding, as the content on LCDs 404 show, the data acquisition of the printing of mini-printer 405, A/D converter 402, the data control by the communicating by letter of RS232 interface 409 and computing machine, stepper motor driver 403 etc.
The course of work of the present invention is as follows:
At first in sample cell 303, put into multifiber 10, sample cell 303 is placed on the one-dimensional scanning platform 5; By sample inlet 301 testing sample solution is sucked in the sample cell 303; By temperature control system 8 with the temperature constant of solution in the sample cell 303 in the required only temperature of biological respinse, identification molecule on the optical fiber 10 and the checking matter in the solution are fully reacted.
After system powers on, LCDs 404 explicit user informations, by the input of keyboard 408, time, setting operation person, the tested biomolecule specy that can revise system be ID number, negative and positive decision content, printing, store and check that testing result, startup " begin to detect " orders etc.
Behind the reaction certain hour, start " beginning to detect " order.Stepper motor driver 403 is subjected to the instruction drive stepping motor 6 of microprocessor chip 401 to drive 5 motions of one-dimensional scanning platform, and its coordinate X gathers the internal data memory that deposits microprocessor chip 401 in by A/D converter 402; The light signal through 9 modulation of high-frequency signal generation circuit that laser instrument 101 sends is injected optical fiber 10 through excitation light path 102, the fluorescent dye on the fibre core surface of optical fiber 10 is through exciting the generation fluorescence signal, this fluorescence signal is converted into the high frequency electrical signal s that is directly proportional with fluorescence signal intensity after entering photodetector 2 by fluorescence receiving light path 103; Carry out related operation with the high-frequency signal r of another road process phase-shift circuit 901 and square wave drive circuit 902 at related operation circuit 7, its result sends into the internal data memory of microprocessor chip 401 by A/D converter 402 afterwards, simultaneously on LCDs 404 respectively with X, Y is as horizontal ordinate, ordinate is drawn the multifiber fluorescence signal absolute value that scanning survey arrives in dye solution in real time, this value compares with the yin and yang attribute decision content of corresponding kind respectively, and then the kind of biomolecule to be measured contained in the sample solution and content made accurately judge, this judged result is also sent into the internal data memory storage, testing result can be exported by the RS232 serial ports.
Specific embodiment:
As shown in Figure 2, laser instrument 101 is a red light semiconductor laser, and its peak wavelength is 635nm, and output power is 10mW, and during detection, the output intensity of semiconductor laser is produced circuit 9 by high-frequency signal and is modulated into the square wave that frequency is 1kHz.Place 4 optical fiber 10 in the sample cell 303, be respectively applied for the three kinds of checking matters such as the plague, anthrax and bird flu that detect in the solution, another root optical fiber is as signal correction.The thermostat temperature of sample cell 303 has 37 ℃ and 42 ℃ two kinds, corresponds respectively to the immune response of antigen and antibody and the hybridization reaction of nucleic acid.Photodetector 2 adopts photomultiplier.
In the correlation detecting circuit, it is the 1kHz symmetrical square wave oscillatory circuit that core is formed by NE555 and d type flip flop CD4013 that high-frequency signal produces circuit 9.Phase-shift circuit adopts two-stage electric current Series Negative Feedback Amplifier circuit, makes the output phase shift of reference signal and measured signal can spend adjustable 0～360 by the variable resistance of regulating wherein.Related operation circuit 7 adopts phase-sensitive detector (PSD) (PSD).Multiplier wherein adopts the switching regulator multiplier that constitutes with field effect transistor, and it has desirable multiplication characteristic and very strong anti-overload ability, is generally adopted.The variable resistor of regulating in the phase-shift circuit 901 makes reference signal and measured signal homophase, thereby makes multiplier be output as maximum.Integrator adopts Active RC low-pass filter (LPF), for improving the output signal-to-noise ratio of correlator, reduces noise bandwidth, but fully suppress multiplier output again with the frequency harmonic component, its integration time constant is chosen RC=12ms.
That described microprocessor chip 401 adopts is the dsp chip MC56F8356 that Freescale company produces, its key component adopts Harvard structure, support parallel processing, under the clock frequency of 60MHz, can reach the execution speed of 60,000,000 instructions, support the instruction of DSP with the C compiler, the program flash memory that has 256K in the sheet, the program storage of 4K, the data flash memory of 8K, the data-carrier store of 16K, 4 * 4 12 modulus converter A/D, dual serial communication interface (SCIs), contain 16 position datawires and address wire, also have 30 I/O mouths in addition, very help the expansion of chip, have the JTAG mouth in addition on the sheet, can be implemented in line programming and debugging, use in the present invention, its speed and function are added the multi-purpose computer that LCDs 404 can replace in the technology formerly fully and are finished data acquisition and signal Processing.407 SL811HST that adopt CYPRESS company to produce of USB chip finally make the communication speed between dsp chip and the USB flash disk can reach 150kbit/s in addition.
Present embodiment is finished the one-time detection required time and is less than 15min, and 4 detection sensitivities to normal concentration solution all reach 10
-12Mol.These performance index all are better than technology formerly.
This detector is in the same place all components and parts and the system integration, and complete machine is integrated, and its volume (length) is 250mm * 220mm * 190mm, and weight is 5kg, can be carried to the field and carry out field quick detection.
Claims (2)
1, a kind of multiprobe fiber optical evanescent wave biotester based on coherent detection, comprise optical system (1), photodetector (2), sample flow path (3), data acquisition and signal processing system (4), one-dimensional scanning platform (5), stepper motor (6) and multifiber (10), described optical system (1) is made up of laser instrument (101), excitation light path (102) and fluorescence receiving light path (103); Described sample flow path (3) is gone out (302) and is formed by sample inlet (301), sample cell (303), sample; Described multifiber (10) one dimension is arranged in the described sample cell (303) equally spacedly, and this sample cell (303) places on the one-dimensional scanning platform (5) that is driven by described stepper motor (6), it is characterized in that also comprising:
One correlation detecting circuit, this correlation detecting circuit comprises that high-frequency signal produces circuit (9) and related operation circuit (7): the output terminal that described high-frequency signal produces circuit (9) links to each other with the control end of described laser instrument (101), described high-frequency signal produces another output terminal of circuit (9) through phase-shift circuit (901), square wave drive circuit (902) connects the reference input of the multiplier circuit (701) of described related operation circuit (7), the signal input part of this multiplier circuit (701) connects the output terminal of described photodetector (2), and the output terminal of this multiplier circuit (701) links to each other with the input end of described data acquisition with signal processing system (4) through the integrator circuit (702) of described related operation circuit (7);
The temperature control system (8) of one control described sample cell (303) temperature, by feed circuit (801), heating element (802), temperature sensor (803) and temperature feedback circuit (804) are formed, feed circuit (801) heat for heating element (802) power supply to described sample cell (303), the temperature of this sample cell (303) is surveyed by temperature sensor (803), the temperature information of the sample cell (303) that the temperature that this temperature sensor (803) is surveyed obtains after temperature feedback circuit (804) output and target temperature are compared is sent into the heating of described feed circuit (801) control heating elements (802) again, with the temperature of control sample cell (303).
2, multiprobe fiber optical evanescent wave biotester based on coherent detection according to claim 1, it is characterized in that described data acquisition and signal processing system (4) be made up of microprocessor chip (401) and peripheral control section thereof, this periphery control section comprises A/D converter (402), stepper motor driver (403), LCDs (404), mini-printer (405), clock chip (406), USB chip and driving circuit thereof (407), keyboard (408), RS232 connects (409), impact damper (410), described microprocessor chip (401) link to each other with above-mentioned each peripheral control section respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100368808A CN101008649B (en) | 2007-01-26 | 2007-01-26 | Multi-probe optical fiber evanescent wave biological detector based on correlation detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100368808A CN101008649B (en) | 2007-01-26 | 2007-01-26 | Multi-probe optical fiber evanescent wave biological detector based on correlation detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101008649A true CN101008649A (en) | 2007-08-01 |
| CN101008649B CN101008649B (en) | 2011-04-06 |
Family
ID=38697180
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100368808A Expired - Fee Related CN101008649B (en) | 2007-01-26 | 2007-01-26 | Multi-probe optical fiber evanescent wave biological detector based on correlation detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101008649B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101701913B (en) * | 2009-11-20 | 2011-03-30 | 清华大学 | Multi-probe optical fiber evanescent wave biological sensor with all-optical-fiber structure |
| CN101246119B (en) * | 2007-03-23 | 2011-06-08 | 国家纳米技术与工程研究院 | Optical fiber biological sensing system |
| CN101799467B (en) * | 2009-02-05 | 2013-10-23 | 深圳迈瑞生物医疗电子股份有限公司 | Particle analyzer, constant-temperature reaction system and constant-temperature reaction method |
| CN105080241A (en) * | 2015-05-28 | 2015-11-25 | 中国科学院上海光学精密机械研究所 | Multi-probe leakage detecting device for efficient filter |
| CN107607514A (en) * | 2017-11-06 | 2018-01-19 | 合肥中科迪沃宁科技有限公司 | A kind of multichannel miRNA fast detectors |
| CN108413994A (en) * | 2018-05-25 | 2018-08-17 | 杭州顿恒科技有限公司 | A kind of power optical fiber sensing device |
| CN110501506A (en) * | 2018-07-05 | 2019-11-26 | 东莞东阳光医疗智能器件研发有限公司 | A kind of biosensor and its application |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6103535A (en) * | 1996-05-31 | 2000-08-15 | University Of Maryland | Optical fiber evanescent field excited fluorosensor and method of manufacture |
| CN1156685C (en) * | 2001-12-26 | 2004-07-07 | 中国科学院上海光学精密机械研究所 | Optical Fiber Evanescent Wave Biosensor |
| CN2674441Y (en) * | 2003-02-28 | 2005-01-26 | 中国科学院上海光学精密机械研究所 | Multi-probe optical fiber wave biosensor |
| CN100470279C (en) * | 2006-06-30 | 2009-03-18 | 清华大学 | Biosensor of full fiber optic evanescent wave |
| CN201016978Y (en) * | 2007-01-26 | 2008-02-06 | 中国科学院上海光学精密机械研究所 | Multi-probe optical fiber evanescent wave biological detector based on correlation detection |
-
2007
- 2007-01-26 CN CN2007100368808A patent/CN101008649B/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101246119B (en) * | 2007-03-23 | 2011-06-08 | 国家纳米技术与工程研究院 | Optical fiber biological sensing system |
| CN101799467B (en) * | 2009-02-05 | 2013-10-23 | 深圳迈瑞生物医疗电子股份有限公司 | Particle analyzer, constant-temperature reaction system and constant-temperature reaction method |
| CN101701913B (en) * | 2009-11-20 | 2011-03-30 | 清华大学 | Multi-probe optical fiber evanescent wave biological sensor with all-optical-fiber structure |
| CN105080241A (en) * | 2015-05-28 | 2015-11-25 | 中国科学院上海光学精密机械研究所 | Multi-probe leakage detecting device for efficient filter |
| CN107607514A (en) * | 2017-11-06 | 2018-01-19 | 合肥中科迪沃宁科技有限公司 | A kind of multichannel miRNA fast detectors |
| CN108413994A (en) * | 2018-05-25 | 2018-08-17 | 杭州顿恒科技有限公司 | A kind of power optical fiber sensing device |
| CN108413994B (en) * | 2018-05-25 | 2024-05-03 | 陕西哲晟电力工程有限公司 | Power optical fiber sensing device |
| CN110501506A (en) * | 2018-07-05 | 2019-11-26 | 东莞东阳光医疗智能器件研发有限公司 | A kind of biosensor and its application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101008649B (en) | 2011-04-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101008649B (en) | Multi-probe optical fiber evanescent wave biological detector based on correlation detection | |
| CN103245641B (en) | Multi-channel planar waveguide evanescent wave biosensor | |
| CN103091486B9 (en) | Test strip detection system | |
| CN101046445B (en) | Linear light beam scanned surface plasma resonant imaging light intensity detection method and system | |
| CN102087214A (en) | Fluorescent quantitative detection instrument | |
| CN201535776U (en) | Quantitative detection system based on test strip marked with constantly illuminating material | |
| CN101231314A (en) | Method and apparatus for testing magnetosensor resonant frequency elasticity | |
| CN206546331U (en) | The fluorescence immunity analyzer of illegal drug in a kind of quick detection food | |
| CN201133898Y (en) | Food safety rapid detector | |
| CN204439552U (en) | A kind of immunofluorescence quantitative analysis instrument | |
| CN108572141A (en) | Composite enhanced optical fiber biosensor and bioprotein molecular concentration detection method | |
| CN102426162A (en) | Fluorescence quantitative detector | |
| CN201016978Y (en) | Multi-probe optical fiber evanescent wave biological detector based on correlation detection | |
| CN203672786U (en) | Dual-wavelength-modulation photoelectric detection device for trace materials | |
| CN1989402B (en) | Single analyte molecule detection | |
| CN1204391C (en) | Method and appaatus for single-laser-sourceharmonic remote sensing gas detection | |
| CN104977281A (en) | High-precision online fluorescence detector and detection method thereof | |
| Huang et al. | A simple optical reader for upconverting phosphor particles captured on lateral flow strip | |
| CN103558177B (en) | Raw meat organizes moisture near-infrared detection apparatus and detection method | |
| CN102087215A (en) | Fluorescent quantitative detection instrument | |
| CN104422669B (en) | Optical waveguide type particle plasma resonance sensing system | |
| CN201440139U (en) | Semi-automatic tubular chemiluminescence immune assay instrument | |
| CN2575678Y (en) | Globe satellite positioning sea chart and fish school detector | |
| CN206906237U (en) | Water quality testing meter | |
| CN207908365U (en) | A kind of data acquisition device of real-time fluorescence quantitative PCR instrument |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110406 Termination date: 20170126 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |