[summary of the invention]
The main technical problem to be solved in the present invention is, a kind of particle analyzer, isothermal reaction system and method are provided, by the fluorescent dye that enters before the reaction tank is taked innovative approach, reduce environment temperature to the impact of measurement result, improve the Stability and veracity of measurement result.
According to an aspect of the present invention, provide a kind of isothermal reaction system, comprising: be used to reactant that the reaction tank of reacting environment is provided; Be used for fluorescent dye is transported to the dyestuff pipe of reaction tank, an end of described dyestuff pipe is communicated with reaction tank, sends dyestuff to reaction tank inside; It is design temperature that thermostatically-controlled equipment, described thermostatically-controlled equipment, reaction tank and dyestuff pipe are configured the temperature that makes described thermostatically-controlled equipment control described reaction tank and dyestuff pipe.
According to a further aspect in the invention, provide a kind of isothermal reaction method, comprise and carry out thermostatically controlled step to entering reaction tank fluorescent dye before.
In accordance with a further aspect of the present invention, provide a kind of particle analyzer, comprise flow chamber and above-mentioned isothermal reaction system, the delivery outlet of described isothermal reaction system is communicated with the entrance of flow chamber, is used for detected material is transported to flow chamber.
The invention has the beneficial effects as follows: the present invention not only carries out thermostatic control to reaction tank according to design temperature, reactant in the reaction tank is reacted under design temperature, also the fluorescent dye that enters before the reaction tank is carried out thermostatic control according to design temperature, thereby heating or the temperature fall time of dyestuff have been prolonged, make the temperature of dyestuff more level off to the temperature of setting, reduced the impact of environment temperature on dyestuff, the Stability and veracity of measurement result is improved.
[embodiment]
By reference to the accompanying drawings the present invention is described in further detail below by embodiment.
In one embodiment, the isothermal reaction system comprises reaction tank, dyestuff pipe and thermostatically-controlled equipment, reaction tank is that reactant (for example reagent and haemocyte) provides the isothermal reaction place, generally comprise mainboard and hatch the pond, hatching the pond is to be positioned at mainboard inside, for example will independently hatch the pond be installed in the cavity of mainboard or the mainboard cavity directly as hatching the pond, hatching the pond can have one or morely, hatches the pond and can be column, spherical or ellipsoid shape.Mainboard is mainly used in providing isoperibol for the reaction of reactant, hatches the pond and provides reacting environment for reactant; The dyestuff pipe is used for carrying fluorescent dye, the one end is communicated with reaction tank, be used for dyestuff is transported in the reaction tank, specifically can make by the pipeline that passes mainboard dyestuff flow at least one and hatch in the pond and mix, react with other reactant (for example other reagent or blood); Thermostatically-controlled equipment is used for the control temperature, by thermostatically-controlled equipment, reaction tank and dyestuff pipe are configured, so that the temperature of thermostatically-controlled equipment control reaction tank and dyestuff pipe is design temperature.
In the present embodiment, not only reaction tank is carried out thermostatic control according to design temperature, reactant in the reaction tank is reacted under design temperature, also the fluorescent dye (being the dyestuff in the dyestuff pipe) that enters before the reaction tank is carried out thermostatic control according to design temperature, thereby obtained following effect: the heating or the temperature fall time that have prolonged dyestuff, make the temperature of dyestuff more level off to the temperature of setting, thereby reduced the impact of environment temperature on dyestuff, the Stability and veracity of measurement result is improved.Temperature control effect can be referring to Fig. 1 and Fig. 2, and wherein horizontal ordinate is the loose direction of side, and ordinate is the fluorescence direction, the complexity of the loose reflection of side cell, fluorescence reflection nucleus amount.Fig. 1 is that dyestuff enters the front scatter diagram without measured same the blood of temperature control measures of reaction tank, about the environment temperature of two figure different, the environment temperature of right figure is higher than the environment temperature of left figure, because dyestuff enters before the reaction tank without temperature control measures, so its temperature can be subject to the impact of environment temperature, know that by Fig. 1 the rising of dye temperature descends before the fluorescence direction is with reaction tank.Fig. 2 is the scatter diagram that dyestuff enters measured same the blood of temperature control measures before the reaction tank, about the environment temperature of two figure different, the environment temperature of right figure is higher than the environment temperature of left figure, because dyestuff has temperature control measures before entering reaction tank, so its temperature is reduced by the impact of environment temperature, thus reduce the fluorescence direction because of reaction tank before the variable quantity that produces of the variation of dye temperature.
When carrying out thermostatic control to entering dyestuff before the reaction tank, can adopt one independently the temperature of thermostatically-controlled equipment control dyestuff pipe be design temperature, adopt again another independently the temperature of thermostatically-controlled equipment control reaction tank be design temperature, be that thermostatically-controlled equipment comprises the first thermostatically-controlled equipment and the second thermostatically-controlled equipment, the first thermostatically-controlled equipment is used for the temperature of control reaction tank, and the second thermostatically-controlled equipment is used for the temperature of control dyestuff pipe.
When carrying out thermostatic control to entering dyestuff before the reaction tank, also can adopt same set of thermostatically-controlled equipment control to enter fluorescent dye (being the dyestuff pipe) before the reaction tank and the temperature of reaction tank is design temperature.
The below describes as example to adopt same set of thermostatically-controlled equipment that dyestuff pipe and reaction tank are carried out temperature control.
Please refer to Fig. 3, in a kind of specific embodiment, the temperature of thermostatically-controlled equipment control reaction tank is design temperature; And be used in and carry fluorescent dye between the dyestuff pipe of reaction tank and reaction tank, to carry out heat interchange.Reaction tank comprise mainboard 1 and be positioned at mainboard 1 at least one hatch pond 3, reagent enters by the pipeline 13 that passes mainboard 1 and hatches the pond and export by the pipeline 14 that passes mainboard 1.For blood cell analyzer, reaction tank provides the isothermal reaction environment for the reaction of reagent and haemocyte, therefore must carry out thermostatic control to reaction tank.In the present embodiment; thermostatically-controlled equipment comprises temperature-control circuit; temperature sensor and thermode; as shown in Figure 4; temperature sensor 8 and thermode 5; 6 link to each other with temperature-control circuit 10 respectively; temperature sensor 8 is installed on the mainboard 1; for example can be attached to the outer wall of mainboard 1 or the inside of inserting mainboard 1; the temperature that is used for the Quorum Sensing pond; the output of temperature-control circuit 10 response temperature sensors 8, control thermode 5; 6 electric power makes thermode 5; 6 change temperature; temperature switch 9 is used for overheat protector; when temperature is higher or lower than certain temperature threshold, control thermode 5; 6 energisings or outage, thus make it reach design temperature.In the embodiment shown in fig. 3, thermode 5,6 contact with the mainboard 1 of reaction tank and and mainboard 1 between carry out heat interchange, the dyestuff pipe is contacted with reaction tank and make dyestuff in the dyestuff pipe and reaction tank between carry out heat interchange, thereby reach the dyestuff pipe and reaction tank shares same set of thermostatically-controlled equipment.For example offer through hole in mainboard 1, the dyestuff pipe inserts in the through hole, and then enters and hatch the pond by entering the pipeline of hatching the pond, carries out heat interchange between dyestuff and the reaction tank thereby make, and realizes entering the thermostatic control of the dyestuff before the reaction tank.For prolonging the temperature control time of dyestuff, can make through hole is curve-like, increases the distance of dye stream warp.
Be illustrated in figure 5 as the temperature control principle of the first thermostatically-controlled equipment, adopt the PID close-loop control mode, with 11 actual temperature value that obtain are as the input of increment type PID controller 12 from temperature sensor from controlled device, process controller 12 resolves and obtains with respect to present actual temperature controlling increment with target temperature.Temperature control is converted into control heat time heating time, the final temperature closed loop control that realizes controlled device 11.
Make the dyestuff pipe contact and make another kind of embodiment such as the Fig. 6 that carries out heat interchange between dyestuff and the reaction tank with reaction tank, shown in 7, the outer wall of mainboard 1 inwardly is provided with groove 15, one end of groove 15 extends to the edge of described mainboard 1 outer wall, the other end extends to dyestuff pipe 4 and the connectivity part of hatching the pond, dyestuff pipe 4 enters from the groove 15 at the edge of mainboard 1 outer wall, be contained in the described groove 15, the other end passes in the groove 15 and is connected with pipeline that the pond is hatched in connection, the one section dyestuff pipe 4 that is positioned at groove 15 is contacted with mainboard 1, and carry out exchange heat between the mainboard, carry out heat interchange between dyestuff and the reaction tank thereby make, realize entering the thermostatic control of the dyestuff before the reaction tank.For prolonging the temperature control time of dyestuff, can make groove is curve-like, for example groove is made into letter " U " or " R " shape, to increase the distance of dye stream warp.In further improved embodiment, comprise that also for the pressure ring 2 that dyestuff pipe 4 is fixed in the groove, shown in Fig. 6,7, dyestuff pipe 4 is fixed on the reaction tank mainboard 1 through pressure ring 2, dyestuff pipe 4 and hatch pond 3 and be communicated with, the dyestuff pipes in the pressure ring 2 are in the control of reaction tank temperature.Fig. 8 is the diagram that dyestuff pipe 4 passes pressure ring 2.Because pressure ring 2 except the fixed dye pipe, has also played the effect (for example, reaction tank mainboard heat being delivered to the dyestuff pipe to add hot dye) of hot transmission.Therefore can adopt the metal material with high thermal conductivity, for example aluminium, aluminium alloy, copper or silver.Smear Heat Conduction Material between dyestuff pipe and pressure ring, the groove, for example heat-conducting silicone grease strengthens the exchange capability of heat between them.
Heated dye volume V is by the sectional area S of dyestuff pipe and be fixed on that dyestuff length of tube L on the reaction tank mainboard determines.Relational expression is V=SL.
In the isothermal reaction system, volume is that thermode was by carrying out heat interchange after the dyestuff of V entered dyestuff pipe on the reaction tank mainboard between reaction tank mainboard and the dyestuff.
Temperature control to dyestuff pipe and reaction tank can be to make it reach design temperature by adding hot dye pipe and reaction tank, also can be by making it reach design temperature to dyestuff pipe and reaction tank cooling.Therefore in above-described embodiment, thermode 5,6 can be heating arrangement, also can be refrigerating plant.Heating arrangement can be attached to heating plate, the heating film on mainboard surface, or heating rod etc.
In above-described embodiment, the reaction tank mainboard is main transferring heat in structure of controlling temperature, therefore can adopt the metal material with high thermal conductivity, for example aluminium, copper or silver.The dyestuff pipe also adopts the material with high thermal conductivity, to improve rate of heat exchange.Reagent and haemocyte are hatched the pond internal reaction constant temperature, and therefore hatching the pond should have preferably chemical stability, can select metal, plastics, pottery etc. to have higher chemical stability material, can use titanium, stainless steel etc. such as connection metal.
In above-described embodiment, reaction tank can also adopt integral structure, one or more cavitys namely are set as hatching the pond in mainboard, offer different through holes for the reactant input and output at the mainboard wall, for example an end and the dyestuff charging through hole with the dyestuff pipe is communicated with, dyestuff is delivered into hatches the pond, as shown in Figure 3.
In certain embodiments, in order further to keep the temperature of reaction tank, in the outside of mainboard or heating plate, the heating film outside also be coated with thermofin 7, shown in Fig. 3,7, thermofin 7 is used for hindering reaction tank and extraneous heat interchange.For example thermofin 7 is bonded in reaction tank mainboard 1 around, be wrapped in heating film, reduced the heat dissipation capacity of heating film to external environment, improved the efficiency of heating surface, also reduced simultaneously the heat dissipation capacity of mainboard 1 to external environment.
Adopt same set of thermostatically-controlled equipment to the other embodiment that dyestuff pipe and reaction tank carry out temperature control can be: thermostatically-controlled equipment comprises temperature-control circuit, temperature sensor and constant temperature oven, temperature sensor links to each other with temperature-control circuit respectively with constant temperature oven, the output of temperature-control circuit response temperature sensor, the control constant temperature oven changes temperature, and reaction tank and dyestuff pipe are positioned in the constant temperature oven, constant temperature oven can be gas tank, controls the temperature of reaction tank and dyestuff pipe with the more uniform air bath of temperature; Also can be water tank, by the thermostatic control of water-bath realization to reaction tank and dyestuff pipe.
The present invention carries out thermostatic control to dyestuff to entering before hatching the pond after dyestuff flows out from dye reservoir, make the temperature of dyestuff as far as possible near design temperature, reduces environment temperature to the impact of dyestuff.
Above-mentioned any embodiment can be applied to comprise cytoanalyze etc. in the various particle analyzers.Particle analyzer comprises flow chamber and isothermal reaction system, and the delivery outlet of isothermal reaction system is communicated with the entrance of flow chamber, is used for that detected material is transported to flow chamber and detects.Testing result outputs to processor and carries out analytic statistics.
Above content is in conjunction with concrete preferred implementation further description made for the present invention, can not assert that implementation of the present invention is confined to these explanations.For the general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some simple deduction or replace, all should be considered as belonging to protection scope of the present invention.