CN101007077B - Composite for oral cavity containing natural plant extracts - Google Patents
Composite for oral cavity containing natural plant extracts Download PDFInfo
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- CN101007077B CN101007077B CN2006100492586A CN200610049258A CN101007077B CN 101007077 B CN101007077 B CN 101007077B CN 2006100492586 A CN2006100492586 A CN 2006100492586A CN 200610049258 A CN200610049258 A CN 200610049258A CN 101007077 B CN101007077 B CN 101007077B
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- China
- Prior art keywords
- folium eriobotryae
- fermented product
- tea polyphenols
- oral cavity
- extract
- Prior art date
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- 210000000214 mouth Anatomy 0.000 title claims description 68
- 239000000419 plant extract Substances 0.000 title claims description 5
- 239000002131 composite material Substances 0.000 title 1
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 73
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 73
- 239000000284 extract Substances 0.000 claims abstract description 67
- 239000000203 mixture Substances 0.000 claims abstract description 40
- 239000006187 pill Substances 0.000 claims abstract description 28
- 239000006189 buccal tablet Substances 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 77
- 241000628997 Flos Species 0.000 claims description 37
- 239000007921 spray Substances 0.000 claims description 35
- 239000002904 solvent Substances 0.000 claims description 34
- 239000002671 adjuvant Substances 0.000 claims description 27
- 229940023488 pill Drugs 0.000 claims description 27
- 241000894006 Bacteria Species 0.000 claims description 21
- 230000003115 biocidal effect Effects 0.000 claims description 13
- 238000001035 drying Methods 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- WCGUUGGRBIKTOS-GPOJBZKASA-N (3beta)-3-hydroxyurs-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CC[C@@H](C)[C@H](C)[C@H]5C4=CC[C@@H]3[C@]21C WCGUUGGRBIKTOS-GPOJBZKASA-N 0.000 claims description 3
- 229940046011 buccal tablet Drugs 0.000 claims description 3
- 229940096998 ursolic acid Drugs 0.000 claims description 3
- PLSAJKYPRJGMHO-UHFFFAOYSA-N ursolic acid Natural products CC1CCC2(CCC3(C)C(C=CC4C5(C)CCC(O)C(C)(C)C5CCC34C)C2C1C)C(=O)O PLSAJKYPRJGMHO-UHFFFAOYSA-N 0.000 claims description 3
- 238000003916 acid precipitation Methods 0.000 claims description 2
- 239000003513 alkali Substances 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims description 2
- 238000010992 reflux Methods 0.000 claims description 2
- 230000002401 inhibitory effect Effects 0.000 claims 9
- 238000000034 method Methods 0.000 abstract description 6
- 230000002265 prevention Effects 0.000 abstract description 4
- 239000006196 drop Substances 0.000 abstract description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 abstract 1
- 244000061508 Eriobotrya japonica Species 0.000 abstract 1
- 235000009008 Eriobotrya japonica Nutrition 0.000 abstract 1
- 244000269722 Thea sinensis Species 0.000 abstract 1
- 230000006806 disease prevention Effects 0.000 abstract 1
- 235000009569 green tea Nutrition 0.000 abstract 1
- 229940041678 oral spray Drugs 0.000 abstract 1
- 239000000668 oral spray Substances 0.000 abstract 1
- 241001122767 Theaceae Species 0.000 description 59
- 238000002156 mixing Methods 0.000 description 32
- 230000000844 anti-bacterial effect Effects 0.000 description 31
- 230000005764 inhibitory process Effects 0.000 description 14
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- 229920000742 Cotton Polymers 0.000 description 6
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- 229920001817 Agar Polymers 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 238000004500 asepsis Methods 0.000 description 3
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- 238000004140 cleaning Methods 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
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- 241000606125 Bacteroides Species 0.000 description 1
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Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to an oral composition, especially to an oral composition containing extracts of natural plants, which is prepared from extracts of green tea polyphenols and loquat leaves. The composition can be prepared into oral spray, buccal tablets or drop pills through conventional processes. The oral composition provided by the present invention has the advantages of oral protection, gum shield, fresh breath, antibacterium, bacteriostasis and disease prevention, etc.; moreover the oral composition is suitable for personal daily oral care, prevention and disinfection.
Description
Technical field
The present invention relates to a kind of composition for oral cavity, relate in particular to a kind of composition for oral cavity that contains natural plant extracts.
Background technology
Current China is along with industry develops rapidly, and various industrial waste gases, waste material produce a large amount of polluter, makes that people live, the pollution of study, working environment is serious day by day, causes the morbidity of various infectious diseases to improve day by day.And the probability that antibacterial, virus are propagated by air, oral cavity route is high.As not paid attention to defence, make various diseases pestilence wink speed draw out, consequence is just hardly imaginable.Some present formulation products generally contain chemical constituent, not only can only play partial action during use, and use the preparation that contains chemical constituent, to people's healthy same formation harm.For this reason, people just replace antibacterial, the bactericide product of chemistry with antibiotic, antibacterial goods in the enough oral cavities that contain natural botanical extraction liquid of serious hope energy all the time.Just be subjected to the favor in the world in recent years along with the effect of Chinese herbal medicine, the various effects in the Chinese herbal medicine constantly are familiar with by world people, accept, and its is nontoxic, it is safer with respect to chemical products to have no side effect, the use that can feel more relieved.
Chinese patent application CN99816880.7, denomination of invention is for disclosing the bacteriostasis of the composition for oral cavity that contains tea polyphenols in ' composition for oral cavity that contains tea polyphenols '.Its weak point is the bacteriostasis after the fungistatic effect of tea polyphenols is worse than tea polyphenols and Folium Eriobotryae fermented product combination.
Summary of the invention
The present invention is directed to existing oral cavity with the defective that contains chemical analysis in antibiotic, the antibacterial goods, propose a kind of antibiotic, antibacterial compositions of using in oral cavity that contains natural plant extracts and more be better than prior art, and can be prepared into mouth sprays, buccal tablet, drop pill.Technical scheme of the present invention is as follows:
Composition for oral cavity of the present invention is characterized in that: every 1000ml or 1000 or 1000 drop pill compositionss are formulated by following component:
Tea polyphenols 5-40g Folium Eriobotryae fermented product 1-40g
All the other are adjuvant.
Composition for oral cavity of the present invention is characterized in that: every 1000ml or 1000 or 1000 drop pill compositionss are formulated by following component:
Tea polyphenols 5-20g Folium Eriobotryae fermented product 1-20g
All the other are adjuvant.
Composition for oral cavity of the present invention is characterized in that: every 1000ml or 1000 or 1000 drop pill compositionss are formulated by following component:
Tea polyphenols 15g Folium Eriobotryae fermented product 2g
All the other are adjuvant.
Composition for oral cavity of the present invention is characterized in that: also added Fructus Anisi Stellati extract, Flos Lonicerae extract, Oleum menthae any or any two, the formulated in combination of three kinds of components forms.
Composition for oral cavity of the present invention is characterized in that: every 1000ml or 1000 or 1000 drop pill compositionss are formulated by following component:
Fructus Anisi Stellati extract 50-200g Flos Lonicerae extract 10-160g
Tea polyphenols 5-40g Folium Eriobotryae fermented product 1-40g
All the other are adjuvant for Oleum menthae 1-20g.
Composition for oral cavity of the present invention is characterized in that: every 1000ml or 1000 or 1000 drop pill compositionss are formulated by following component:
Fructus Anisi Stellati extract 50-150g Flos Lonicerae extract 10-80g
Tea polyphenols 5-20g Folium Eriobotryae fermented product 1-20g
All the other are adjuvant for Oleum menthae 1-10g.
Composition for oral cavity of the present invention is characterized in that: every 1000ml or 1000 or 1000 drop pill compositionss are formulated by following component:
Fructus Anisi Stellati extract 75g Flos Lonicerae extract 20g
Tea polyphenols 15g Folium Eriobotryae fermented product 2g
All the other are adjuvant for Oleum menthae 2g.
Composition for oral cavity of the present invention is characterized in that: adjuvant is that water is formed by correctives 10-100g, solubilizing agent 10-50g, surplus in every 1000ml compositions, makes mouth sprays through conventional technology.
Composition for oral cavity of the present invention is characterized in that: described compositions is made buccal tablet through conventional technology.
Composition for oral cavity of the present invention is characterized in that: described compositions is made drop pill through conventional technology.
Folium Eriobotryae fermented product among the present invention, Flos Lonicerae extract, Fructus Anisi Stellati extract are can directly buy on the market, and its extracting method is:
Folium Eriobotryae fermented product: this product be Folium Eriobotryae behind alcohol reflux, carry acid precipitation, drying through alkali again and make.Contain ursolic acid at least 25%.
Flos Lonicerae extract: this product is that Flos Lonicerae is through water extract-alcohol precipitation, concentrated getting.
Fructus Anisi Stellati extract: the ethanol lixiviate, concentrate and get.
The present composition confirms through in-vitro antibacterial (antibacterial) effect: to escherichia coli, and staphylococcus aureus, Staphylococcus albus, Streptococcus mutans, Streptococcus sanguis, actinomyces viscosus, beta hemolytic streptococcuss etc. all have inhibition preferably, killing action.The present invention adopts natural botanical extraction liquid, makes harmlessly, is of value to healthy a kind of environmental protection goods.Have the protection oral cavity, tooth protection, fresh breath, antibiotic, antibacterial, effects such as pestilence prevention.The present invention is suitable for individual's daily mouth care, prevention and sterilization and uses.
The present invention through tea polyphenols, Folium Eriobotryae fermented product, with the combination of tea polyphenols and Folium Eriobotryae fermented product, the comparative test of three's fungistatic effect, the result shows: the fungistatic effect of the combination of tea polyphenols and Folium Eriobotryae fermented product is better than the fungistatic effect of tea polyphenols or Folium Eriobotryae fermented product.
The specific embodiment
The invention will be further described below in conjunction with embodiment.But they are not limitation of the invention.
Embodiment 1
Tea polyphenols, Folium Eriobotryae fermented product, with the combination of tea polyphenols and Folium Eriobotryae fermented product, the comparative test of three's fungistatic effect:
Material
1. for test strain: staphylococcus aureus, escherichia coli, good fortune Sai Shi bacteroid, the general Salmonella of middle type.
2. tea polyphenols: content is〉90% light yellowish brown powder.Be made into 1.5% solution as sample with sterilized water;
Folium Eriobotryae fermented product: contain the ursolic acid amount and be 25% yellow powder.Be made into 0.2% solution as sample with sterilized water;
The combination of tea polyphenols and Folium Eriobotryae fermented product: tea polyphenols 15g, Folium Eriobotryae fermented product 2g are made into 1000ml solution as sample with sterilized water.
With sterilized water in contrast.
3. method is utilized this product constantly to dissolve through agar diffusion and is formed the variable concentrations gradient, to show its bacteriostasis.Test is big or small to judge whether it has bacteriostasis by antibacterial ring.
Operation sequence
(1) preparation of bacteria inhibition tablet: get aseptic and exsiccant filter paper.Every drips actual working concentration this product 20 μ l, filter paper is lain against in the aseptic plate of cleaning then, uncaps to put in the incubator (37 ℃) and dries, or put under the room temperature standby behind the natural drying.
(2) preparation of negative control print: get aseptic dry filter paper sheet, every drips sterile distilled water 20 μ l, and dry back is standby.
(3) inoculation of test organisms: dip in aseptic cotton swab that to get concentration be 5 * 105cfu/ml～5 * 106cfu/ml staphylococcus aureus (ATCC6538) bacteria suspension, evenly smear 3 times at the nutrient agar planar surface.Whenever smear 1 time, flat board should rotate 60 °, at last cotton swab is smeared a week around plate edge.Build plate, put drying at room temperature 5min.
(4) print pastes and puts: each test is pasted and is put 1 microbiological contamination flat board, and each dull and stereotyped subsides is put 4 test prints, 1 negative control print, totally 5.Be put in planar surface with the subsides of aseptic nipper coupongs.Between each print center at a distance of more than the 25mm, with the periphery of flat board at a distance of more than the 15mm.After subsides are put well, gently press print, make it be close to planar surface with aseptic nipper.Build plate, put 37 ℃ of incubators, cultivate 16h～18h observed result.Diameter (comprising paster) and record with the antibacterial ring of vernier caliper measurement.Test repeats 3 times.
When measuring antibacterial ring, should select evenly and the antibacterial ring of integral asepsis growth carries out.Measuring its diameter should be the boundary with antibacterial ring outer.
Estimate the judgement of regulation (1) bacteriostasis:
Inhibition zone diameter has been judged to bacteriostasis greater than 7mm person.
Inhibition zone diameter is less than or equal to 7mm person, is judged to no bacteriostasis.
(2) 3 times repeated trials all has bacteriostasis person as a result, and it is qualified to be judged to.
(3) negative control group should not have antibacterial ring generation.Otherwise invalidate the test.
The results are shown in following table:
The result shows: the fungistatic effect of the combination of tea polyphenols and Folium Eriobotryae fermented product is better than the fungistatic effect of tea polyphenols or Folium Eriobotryae fermented product.
Embodiment 2
Select tea polyphenols 5g for use, Folium Eriobotryae fermented product 1g, solubilizing agent 10g, correctives 10g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 3
Select tea polyphenols 40g for use, Folium Eriobotryae fermented product 40g, solubilizing agent 50g, correctives 100g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 4
Select tea polyphenols 15g for use, Folium Eriobotryae fermented product 2g, solubilizing agent 25g, correctives 50g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 5
Select tea polyphenols 20g for use, Folium Eriobotryae fermented product 20g adds adjuvant, makes 1000 of buccal tablets of the present invention through conventional technology.
Embodiment 6
Select tea polyphenols 15g for use, Folium Eriobotryae fermented product 2g adds adjuvant, makes drop pill 1000 drop pill of the present invention through conventional technology.
Embodiment 7
Select tea polyphenols 5g for use, Folium Eriobotryae fermented product 1g, Fructus Anisi Stellati extract 50g, solubilizing agent 10g, correctives 10g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 8
Select tea polyphenols 40g for use, Folium Eriobotryae fermented product 40g, Fructus Anisi Stellati extract 200g, solubilizing agent 50g, correctives 100g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 9
Select tea polyphenols 15g for use, Folium Eriobotryae fermented product 2g, Fructus Anisi Stellati extract 75g, solubilizing agent 25g, correctives 50g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 10
Select tea polyphenols 15g for use, Folium Eriobotryae fermented product 2g, Fructus Anisi Stellati extract 75g adds adjuvant, makes 1000 of buccal tablets of the present invention through conventional technology.
Embodiment 11
Select tea polyphenols 20g for use, Folium Eriobotryae fermented product 20g, Fructus Anisi Stellati extract 150g adds adjuvant, makes drop pill 1000 drop pill of the present invention through conventional technology.
Embodiment 12
Select tea polyphenols 5g for use, Folium Eriobotryae fermented product 1g, Flos Lonicerae extract 10g, solubilizing agent 10g, correctives 10g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 13
Select tea polyphenols 40g for use, Folium Eriobotryae fermented product 40g, Flos Lonicerae extract 160g, solubilizing agent 50g, correctives 100g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 14
Select tea polyphenols 15g for use, Folium Eriobotryae fermented product 2g, Flos Lonicerae extract 20g, solubilizing agent 25g, correctives 50g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 15
Select tea polyphenols 15g for use, Folium Eriobotryae fermented product 2g, Flos Lonicerae extract 20g adds adjuvant, makes 1000 of buccal tablets of the present invention through conventional technology.
Embodiment 16
Select tea polyphenols 20g for use, Folium Eriobotryae fermented product 20g, Flos Lonicerae extract 80g adds adjuvant, makes drop pill 1000 drop pill of the present invention through conventional technology.
Embodiment 17
Select tea polyphenols 5g for use, Folium Eriobotryae fermented product 1g, Oleum menthae 1g, solubilizing agent 10g, correctives 10g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 18
Select tea polyphenols 40g for use, Folium Eriobotryae fermented product 40g, Oleum menthae 20g, solubilizing agent 50g, correctives 100g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 19
Select tea polyphenols 15g for use, Folium Eriobotryae fermented product 2g, Oleum menthae 2g, solubilizing agent 25g, correctives 50g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 20
Select tea polyphenols 5g for use, Folium Eriobotryae fermented product 1g, Oleum menthae 1g adds adjuvant, makes 1000 of buccal tablets of the present invention through conventional technology.
Embodiment 21
Select tea polyphenols 15g for use, Folium Eriobotryae fermented product 2g, Oleum menthae 2g adds adjuvant, makes drop pill 1000 drop pill of the present invention through conventional technology.
Embodiment 22
Select tea polyphenols 5g for use, Folium Eriobotryae fermented product 1g, Fructus Anisi Stellati extract 50g, Flos Lonicerae extract 10g, solubilizing agent 10g, correctives 10g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 23
Select tea polyphenols 40g for use, Folium Eriobotryae fermented product 40g, Fructus Anisi Stellati extract 200g, Flos Lonicerae extract 160g, solubilizing agent 50g, correctives 100g, after adding suitable quantity of water dissolving mixing, add water to 1000ml again and make mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 24
Select tea polyphenols 15g for use, Folium Eriobotryae fermented product 2g, Fructus Anisi Stellati extract 75g, Flos Lonicerae extract 20g, solubilizing agent 25g, correctives 100g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 25
Select tea polyphenols 20g for use, Folium Eriobotryae fermented product 20g, Fructus Anisi Stellati extract 150g, Flos Lonicerae extract 80g, solubilizing agent 30g, correctives 60g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 26
Select tea polyphenols 5g for use, Folium Eriobotryae fermented product 1g, Fructus Anisi Stellati extract 50g, Flos Lonicerae extract 10g adds adjuvant, makes 1000 of buccal tablets of the present invention through conventional technology.
Embodiment 27
Select tea polyphenols 5g for use, Folium Eriobotryae fermented product 1g, Flos Lonicerae extract 10g, Oleum menthae 1g, solubilizing agent 10g, correctives 10g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 28
Select tea polyphenols 40g for use, Folium Eriobotryae fermented product 40g, Flos Lonicerae extract 160g, Oleum menthae 20g, solubilizing agent 50g, correctives 100g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 29
Select tea polyphenols 20g for use, Folium Eriobotryae fermented product 20g, Flos Lonicerae extract 80g, Oleum menthae 10g, solubilizing agent 30g, correctives 60g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 30
Select tea polyphenols 15g for use, Folium Eriobotryae fermented product 2g, Flos Lonicerae extract 20g, Oleum menthae 2g, solubilizing agent 25g, correctives 50g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 31
Select tea polyphenols 5g for use, Folium Eriobotryae fermented product 1g, Flos Lonicerae extract 10g, Oleum menthae 1g adds adjuvant, makes drop pill 1000 drop pill of the present invention through conventional technology.
Embodiment 32
Select tea polyphenols 5g for use, Folium Eriobotryae fermented product 1g, Fructus Anisi Stellati extract 50g, Oleum menthae 1g, solubilizing agent 10g, correctives 10g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 33
Select tea polyphenols 40g for use, Folium Eriobotryae fermented product 40g, Fructus Anisi Stellati extract 200g, Oleum menthae 20g, solubilizing agent 50g, correctives 100g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 34
Select tea polyphenols 20g for use, Folium Eriobotryae fermented product 20g, Fructus Anisi Stellati extract 150g, Oleum menthae 10g, solubilizing agent 30g, correctives 60g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 35
Select tea polyphenols 15g for use, Folium Eriobotryae fermented product 2g, Fructus Anisi Stellati extract 75g, Oleum menthae 2g, solubilizing agent 25g, correctives 50g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 36
Select tea polyphenols 20g for use, Folium Eriobotryae fermented product 20g, Fructus Anisi Stellati extract 150g, Oleum menthae 10g adds adjuvant, makes 1000 of buccal tablets of the present invention through conventional technology.
Embodiment 37
Select tea polyphenols 5g for use, Folium Eriobotryae fermented product 1g, Flos Lonicerae extract 10g, Oleum menthae 1g, solubilizing agent 10g, correctives 10g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 38
Select tea polyphenols 40g for use, Folium Eriobotryae fermented product 40g, Flos Lonicerae extract 160g, Oleum menthae 20g, solubilizing agent 50g, correctives 100g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 39
Select tea polyphenols 20g for use, Folium Eriobotryae fermented product 20g, Flos Lonicerae extract 80g, Oleum menthae 10g, solubilizing agent 30g, correctives 60g after adding suitable quantity of water dissolving and mixing, adds water to 000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 40
Select tea polyphenols 15g for use, Folium Eriobotryae fermented product 2g, Flos Lonicerae extract 20g, Oleum menthae 2g, solubilizing agent 25g, correctives 50g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 41
Select tea polyphenols 5g for use, Folium Eriobotryae fermented product 1g, Flos Lonicerae extract 10g, Oleum menthae 1g adds adjuvant, makes drop pill 1000 drop pill of the present invention through conventional technology.
Embodiment 42
Select tea polyphenols 5g for use, Folium Eriobotryae fermented product 1g, Fructus Anisi Stellati extract 50g, Flos Lonicerae extract 10g, Oleum menthae 1g, solubilizing agent 10g, correctives 10g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 43
Select tea polyphenols 40g for use, Folium Eriobotryae fermented product 40g, Fructus Anisi Stellati extract 200g, Flos Lonicerae extract 160g, Oleum menthae 20g, solubilizing agent 50g, correctives 100g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 44
Select tea polyphenols 20g for use, Folium Eriobotryae fermented product 20g, Fructus Anisi Stellati extract 150g, Flos Lonicerae extract 80g, Oleum menthae 10g, solubilizing agent 30g, correctives 60g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 45
Select tea polyphenols 15g for use, Folium Eriobotryae fermented product 2g, Fructus Anisi Stellati extract 75g, Flos Lonicerae extract 20g, Oleum menthae 2g, solubilizing agent 25g, correctives 50g after adding suitable quantity of water dissolving and mixing, adds water to 1000ml again and makes mouth sprays 1000ml of the present invention through conventional technology.
Embodiment 46
Select tea polyphenols 20g for use, Folium Eriobotryae fermented product 20g, Fructus Anisi Stellati extract 150g, Flos Lonicerae extract 80g, Oleum menthae 10g adds adjuvant, makes 1000 of buccal tablets of the present invention through conventional technology.
Embodiment 47
Adopt the foregoing description 2,7,12,21,22,25,31,44 products obtained therefroms to carry out in-vitro antibacterial (antibacterial) test.
Anti-(pressing down) bacterium test of staphylococcus aureus
Purpose is measured this product to anti-(pressing down) bacterium effect of staphylococcus aureus.
Method is utilized this product constantly to dissolve through agar diffusion and is formed the variable concentrations gradient, to show its bacteriostasis.Test is big or small to judge whether it has bacteriostasis by antibacterial ring.
Operation sequence
(1) preparation of bacteria inhibition tablet: get aseptic and exsiccant filter paper.Every drips actual working concentration this product 20 μ l, filter paper is lain against in the aseptic plate of cleaning then, uncaps to put in the incubator (37 ℃) and dries, or put under the room temperature standby behind the natural drying.
(2) preparation of negative control print: get aseptic dry filter paper sheet, every drips sterile distilled water 20 μ l, and dry back is standby.
(3) inoculation of test organisms: dip in aseptic cotton swab that to get concentration be 5 * 10
5Cfu/ml～5 * 10
6Cfu/ml staphylococcus aureus (ATCC6538) bacteria suspension is evenly smeared 3 times at the nutrient agar planar surface.Whenever smear 1 time, flat board should rotate 60 °, at last cotton swab is smeared a week around plate edge.Build plate, put drying at room temperature 5min.
(4) print pastes and puts: each test is pasted and is put 1 microbiological contamination flat board, and each dull and stereotyped subsides is put 4 test prints, 1 negative control print, totally 5.Be put in planar surface with the subsides of aseptic nipper coupongs.Between each print center at a distance of more than the 25mm, with the periphery of flat board at a distance of more than the 15mm.After subsides are put well, gently press print, make it be close to planar surface with aseptic nipper.Build plate, put 37 ℃ of incubators, cultivate 16h～18h observed result.Diameter (comprising paster) and record with the antibacterial ring of vernier caliper measurement.Test repeats 3 times.
When measuring antibacterial ring, should select evenly and the antibacterial ring of integral asepsis growth carries out.Measuring its diameter should be the boundary with antibacterial ring outer.
Estimate the judgement of regulation (1) bacteriostasis:
Inhibition zone diameter has been judged to bacteriostasis greater than 7mm person.
Inhibition zone diameter is less than or equal to 7mm person, is judged to no bacteriostasis.
(2) 3 times repeated trials all has bacteriostasis person as a result, and it is qualified to be judged to.
(3) negative control group should not have antibacterial ring generation.Otherwise invalidate the test.
4 bacteria inhibition tablet inhibition zone diameters are all greater than 7mm as a result, and the negative control scraps of paper do not have antibacterial ring.
The conclusion sample all has bacteriostasis to staphylococcus aureus.
Anti-(pressing down) bacterium test of escherichia coli
Purpose is measured this product to colibacillary anti-(pressing down) bacterium effect.
Method is utilized this product constantly to dissolve through agar diffusion and is formed the variable concentrations gradient, to show its bacteriostasis.Test is big or small to judge whether it has bacteriostasis by antibacterial ring.
(1) staphylococcus aureus (ATCC6538), escherichia coli (8099)
Operation sequence
(1) preparation of bacteria inhibition tablet: get aseptic and exsiccant filter paper.Every drips actual working concentration this product 20 μ l, filter paper is lain against in the aseptic plate of cleaning then, uncaps to put in the incubator (37 ℃) and dries, or put under the room temperature standby behind the natural drying.
(2) preparation of negative control print: get aseptic dry filter paper sheet, every drips sterile distilled water 20 μ l, and dry back is standby.
(3) inoculation of test organisms: dip in aseptic cotton swab that to get concentration be 5 * 10
5Cfu/ml～5 * 10
6Cfu/ml escherichia coli (8099) bacteria suspension is evenly smeared 3 times at the nutrient agar planar surface.Whenever smear 1 time, flat board should rotate 60 °, at last cotton swab is smeared a week around plate edge.Build plate, put drying at room temperature 5min.
(4) print pastes and puts: each test is pasted and is put 1 microbiological contamination flat board, and each dull and stereotyped subsides is put 4 test prints, 1 negative control print, totally 5.Be put in planar surface with the subsides of aseptic nipper coupongs.Between each print center at a distance of more than the 25mm, with the periphery of flat board at a distance of more than the 15mm.After subsides are put well, gently press print, make it be close to planar surface with aseptic nipper.Build plate, put 37 ℃ of incubators, cultivate 16h～18h observed result.Diameter (comprising paster) and record with the antibacterial ring of vernier caliper measurement.Test repeats 3 times.
When measuring antibacterial ring, should select evenly and the antibacterial ring of integral asepsis growth carries out.Measuring its diameter should be the boundary with antibacterial ring outer.
Estimate the judgement of regulation (1) bacteriostasis:
Inhibition zone diameter has been judged to bacteriostasis greater than 7mm person.
Inhibition zone diameter is less than or equal to 7mm person, is judged to no bacteriostasis.
(2) 3 times repeated trials all has bacteriostasis person as a result, and it is qualified to be judged to.
(3) negative control group should not have antibacterial ring generation.Otherwise invalidate the test.
4 bacteria inhibition tablet inhibition zone diameters are all greater than 7mm as a result, and the negative control scraps of paper do not have antibacterial ring.
The conclusion sample all has bacteriostasis to escherichia coli.
Embodiment 48
Adopt the foregoing description 5,11,15,19,23,31,33,40,46 products obtained therefroms to carry out mucomembranous irritant test
The Holland white rabbit is provided by Zhejiang Province Disease Control and Prevention Center.Get 20 female Holland pure lines white rabbits, body weight 2.5-3Kg is divided into two groups at random.10 of test group, 10 of matched groups, test group vagina administration every day three times, each 1ml; Matched group vagina every day is given distilled water three times, and each 1ml all one week of continuous application, observes vaginal secretions every day.Experimental result: two treated animal vaginas all do not have any abnormal secretion thing, and pudendum does not all have redness.The back execution of one week, perusal: two treated animal vaginal mucosaes are smooth, no petechia and edema, it is complete that the pathological section mirror is observed mucosa down, no cell infiltration, no meat dental tissue hypertrophy.Result of the test shows that oral cavity composition of the present invention does not have any stimulation to skin.
Embodiment 49
Adopt the foregoing description 2,7,11,15,20,24,33,40,43 products obtained therefroms to carry out acute toxicity test
Get 50 of white mice, male and female half and half, body weight 20-22g, be divided into 10 of matched groups at random, 40 of test control group, every day 3 times, the gastrointestinal oral administration, each 1ml is 15000 times that the adult measures, matched group 3 oral isodose distilled water every day in one week of logotype, are observed the animal state, put to death after seven days, the naked eyes mirror is observed the heart, liver, nephridial tissue pathological change down.
The result is in the medication process, the two treated animal hearts, and liver, the kidney tunicle is smooth, and no any damage is organized in no petechia.Result of the test shows that oral cavity composition of the present invention does not have any toxic action to skin.
Claims (9)
1. an oral cavity of containing natural plant extracts is characterized in that with antibiotic, bacteria inhibiting composition: every 1000ml or 1000 or 1000 drop pill compositionss are formulated by following component:
Tea polyphenols 5-40g Folium Eriobotryae fermented product 1-40g
All the other are adjuvant; Described Folium Eriobotryae fermented product, is carried acid precipitation, drying through alkali again and is made behind alcohol reflux by Folium Eriobotryae, wherein contains ursolic acid at least 25% by weight percentage.
2. oral cavity according to claim 1 is characterized in that with antibiotic, bacteria inhibiting composition: every 1000ml or 1000 or 1000 drop pill compositionss are formulated by following component:
Tea polyphenols 5-20g Folium Eriobotryae fermented product 1-20g
All the other are adjuvant.
3. oral cavity according to claim 1 is characterized in that with antibiotic, bacteria inhibiting composition: every 1000ml or 1000 or 1000 drop pill compositionss are formulated by following component:
Tea polyphenols 15g Folium Eriobotryae fermented product 2g
All the other are adjuvant.
4. oral cavity according to claim 1 is with antibiotic, bacteria inhibiting composition, it is characterized in that every 1000ml or 1000 or 1000 drop pill compositionss are formulated by following component:
Fructus Anisi Stellati extract 50-200g Flos Lonicerae extract 10-160g
Tea polyphenols 5-40g Folium Eriobotryae fermented product 1-40g
All the other are adjuvant for Oleum menthae 1-20g;
Described Flos Lonicerae extract is Flos Lonicerae through water extract-alcohol precipitation, concentrate and get, and described Fructus Anisi Stellati extract is ethanol lixiviate, concentrated getting.
5. oral cavity according to claim 4 is characterized in that with antibiotic, bacteria inhibiting composition: every 1000ml or 1000 or 1000 drop pill compositionss are formulated by following component:
Fructus Anisi Stellati extract 50-150g Flos Lonicerae extract 10-80g
Tea polyphenols 5-20g Folium Eriobotryae fermented product 1-20g
All the other are adjuvant for Oleum menthae 1-10g.
6. oral cavity according to claim 4 is characterized in that with antibiotic, bacteria inhibiting composition: every 1000ml or 1000 or 1000 drop pill compositionss are formulated by following component:
Fructus Anisi Stellati extract 75g Flos Lonicerae extract 20g
Tea polyphenols 15g Folium Eriobotryae fermented product 2g
All the other are adjuvant for Oleum menthae 2g.
According to each described oral cavity in the claim 1 to 6 with antibiotic, bacteria inhibiting composition, it is characterized in that: adjuvant is that water is formed by correctives 10-100g, solubilizing agent 10-50g, surplus in every 1000ml compositions, makes mouth sprays through conventional technology.
According to each described oral cavity in the claim 1 to 6 with antibiotic, bacteria inhibiting composition, it is characterized in that:
Described compositions is made buccal tablet through conventional technology.
According to each described oral cavity in the claim 1 to 6 with antibiotic, bacteria inhibiting composition, it is characterized in that:
Described compositions is made drop pill through conventional technology.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100492586A CN101007077B (en) | 2006-01-25 | 2006-01-25 | Composite for oral cavity containing natural plant extracts |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100492586A CN101007077B (en) | 2006-01-25 | 2006-01-25 | Composite for oral cavity containing natural plant extracts |
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| CN101007077B true CN101007077B (en) | 2011-10-26 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP2192910A1 (en) * | 2007-10-01 | 2010-06-09 | Colgate-Palmolive Company | Oral compositions containing botanical extracts |
| EP2689807A1 (en) * | 2009-12-04 | 2014-01-29 | Colgate-Palmolive Company | Oral compositions containing extracts of zizyphus joazeiro and related methods |
| CN106562921B (en) * | 2016-11-02 | 2020-11-24 | 李浩然 | A liquid oral composition containing Chinese medicinal active substances and tea tree essential oil |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1086669A (en) * | 1992-11-11 | 1994-05-18 | 李宗相 | A kind of health loquat tea and compound method thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1086669A (en) * | 1992-11-11 | 1994-05-18 | 李宗相 | A kind of health loquat tea and compound method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 张玉等.枇杷的营养及功能成分研究进展.食品科学.2005,26(9),602-604. * |
| 朱景委.药茶的炮制与饮用.饮食科学.1995,(4),26-27. * |
| 毕彩虹等.茶叶保健制品的开发利用.四川农业科技.2005,(7),37-38. * |
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Denomination of invention: Composite for oral cavity containing natural plant extracts Effective date of registration: 20171124 Granted publication date: 20111026 Pledgee: Bank of Hangzhou Limited by Share Ltd science and Technology Branch Pledgor: Hangzhou Lion Biotechnology Co., Ltd. Registration number: 2017330000196 |
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