CN101003829A - Polypeptide of seed of spine date, preparation method, and application - Google Patents
Polypeptide of seed of spine date, preparation method, and application Download PDFInfo
- Publication number
- CN101003829A CN101003829A CNA2007100614268A CN200710061426A CN101003829A CN 101003829 A CN101003829 A CN 101003829A CN A2007100614268 A CNA2007100614268 A CN A2007100614268A CN 200710061426 A CN200710061426 A CN 200710061426A CN 101003829 A CN101003829 A CN 101003829A
- Authority
- CN
- China
- Prior art keywords
- seed
- polypeptide
- spine date
- collagen protein
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
This invention provides a method for preparing Ziziphus jujuba peptides and their application. The Ziziphus jujuba peptides are a mixture of water-soluble small molecular peptides by combination of Ziziphus jujuba and collagen, thus can largely improve the absorption rate by human bodies. The Ziziphus jujuba peptides can improve sleep, and have such advantages as high curative effect, high absorbency, rapid acting, more addiction, and no toxic or side effects. The method comprises: mixing macromolecular collagen with Ziziphus jujuba powder, and enzymolyzing to obtain small molecular peptide. The method is simple yet practicable. The Ziziphus jujuba peptides can be used to produce food, healthcare food and medicine.
Description
Technical field
The present invention relates to food, heath food, medicine and preparing technical field thereof.
Background technology
Spina Date Seed is one of herbal medicine of medicine-food two-purpose.In traditional Chinese medicine, calm the nerves as having, the Chinese medicine of the peaceful heart, calmness, improvement sleep, but generally want the plurality of Chinese compatibility to use, and consumption is big, and must process boils or complicated procedures of forming such as pill.Its effective ingredient is not made that micromolecule polypeptide uses as protective foods.It eats and the equal end of pharmaceutical use is brought into play well.
Summary of the invention
Polypeptide of seed of spine date, Preparation method and use have been the purpose of this invention is to provide, polypeptide of seed of spine date is a Spina Date Seed and collagen protein bonded polypeptide small molecule mixture soluble in water, have calm and improve the sleep effect, this eliminate effect height, human absorptivity's height, functional component play a role soon, use easy to carry, take not habituation, without any side effects for a long time; Its method for making uniqueness is simple and practical; Polypeptide of seed of spine date especially can carry out widespread use in food, protective foods or medicine.
One of the present invention has provided a kind of new material: polypeptide of seed of spine date is characterized in that it is a Spina Date Seed and collagen protein bonded polypeptide small molecule mixture soluble in water; The absorption of human body and the curative effect thereof that have greatly promoted these two kinds of materials of Spina Date Seed and collagen protein are worth, have calm and improve the sleep effect, effect height, human absorptivity's height, functional component play a role soon, take for a long time habituation not, characteristics without any side effects.
The present invention's two has provided a kind of preferable preparation method of this novel substance of polypeptide of seed of spine date, it is characterized in that the method with enzymolysis, is about to macromolecular collagen protein and mixes the method that enzymolysis also obtains small-molecular peptides with spiny jujuba seed powder.
Described enzymolysis process is preferably: bright bone (animal fresh bones such as ox, sheep, pig) is extracted through steaming and decocting under high pressure collagen protein → enzymolysis → also filtration can make with spiny jujuba seed powder mixing → accents pH value to 7.2 ~ 8.5 → carry out.
Can be after the intact filtering and concentrating of above-mentioned enzymolysis again through super-dry.It is good using the filtration of sheet frame pressing machine, vacuum concentration and spraying drying, to produce dry powder doses.
The temperature that collagen protein is extracted in the bright bone steaming and decocting under high pressure of above-mentioned ox is that 100 ~ 125 ℃, pressure are that 1 ~ 3MPa is good.
Above-mentioned spiny jujuba seed powder can be with 120 order powder, also available micron powder etc.
Above-mentioned collagen protein and spiny jujuba seed powder mixed weight portion rate (dry-matter than), collagen protein: spiny jujuba seed powder=39 ~ 41: 59 ~ 61 is good, during the said extracted collagen protein, and to make concentration of substrate be 5 ~ 9% preferable.
Above-mentioned accent pH value, it is good transferring pH value with citric acid and food grade ammoniacal liquor, adjustment such as also available acetic acid and edible alkali.
To carry out enzymolysis be good to add compound protease during above-mentioned enzymolysis, add-on be substrate gross weight (comprising collagen egg, water, spiny jujuba seed powder etc.) 0.8 ~ 5% for good, enzymolysis time was good with 4 ~ 5 hours.
The present invention's three has provided the purposes of this novel substance of polypeptide of seed of spine date: can be used for making the goods such as food, protective foods or medicine with sedative action.
For example, can be made into chewing tablet, electuary or the capsule made from method of Chinese medicinal; Also available method for making food food prepared therefrom.
It is good that system chewing tablet has following components by weight percent umber proportioning, polypeptide of seed of spine date: releasing agent: seasonings=100: 1 ~ 2: 0.7 ~ 1.3.
Mechanism of the present invention is: polypeptide of seed of spine date is meant Spina Date Seed and collagen protein bonded polypeptide mixture, and is for the small-molecular peptides material, soluble in water.Spina Date Seed occurs with polypeptide form, and and the collagen protein combination, played the effect of mutually promoting, greatly promoted the absorptivity of these two kinds of materials and curative effect thereof and be worth, can make the curative effect of Spina Date Seed appear suddenly out, very easily by the abundant rapid absorption of human body.After polypeptide of seed of spine date enters human body, need not digest again and can directly be absorbed, reduce acidolysis and enzymolysis process in stomach, so to keeping its curative effect composition to have crucial meaning by stomach and intestine.Owing to increased the effect of Spina Date Seed greatly, make the effective constituent in the Spina Date Seed obtain maximum performance, thus its consumption is reduced, and easy to use.Do raw material with polypeptide of seed of spine date, be pressed into various oral preparation such as chewing tablet, have the better effects of sedative health-care medicine-food two-purpose.Spina Date Seed with the directed cutting means of biological enzymolysis, can be converted into the macromolecular substance of these two kinds of raw materials the micromolecule polypeptide material, make the molecular change of Spina Date Seed get very little (3000D ~ 50D), can make the effective constituent in the Spina Date Seed obtain maximum performance through enzymolysis.This also is an innovative approach, and the simple and feasible practicality is a kind of preferable method for making.The polypeptide of seed of spine date product is a kind of white or micro-yellow powder, makes protective foods or medicament medicine with it as raw material.Evidence has outstanding calmness and improves the effect of sleeping, and effect is remarkable.
The positive unusual effect that the present invention has is: polypeptide of seed of spine date sedative health-care oral preparation of the present invention, effective constituent is polypeptide of seed of spine date, people through nearly hundred insomnias and poor sleeping quality tries clothes, have remarkable calmness and improve the sleep effect, good health care effect, do not add Western medicine, take not habituation for a long time, have effect height, human absorptivity's height, make functional component play a role fast, use easy to carry, without any side effects, the ripe simple uniqueness of method for making, resource wide, can save outstanding features such as a large amount of drug resources, cost are low.Its method for making uniqueness is simple and practical.But the polypeptide of seed of spine date medicine-food two-purpose especially can carry out widespread use in goods such as food, protective foods or medicine.
Be described further below in conjunction with embodiment, but not as a limitation of the invention.
Embodiment
One, polypeptide of seed of spine date (mixture) preparation method (with above-mentioned) is for example:
1, Spina Date Seed is sieved the impurity elimination thing, pulverize with pulverizing mill;
2, extract collagen protein.Get the bright bone required amount (as 40kg) of ox, add water, as can be 60% (60kg) of total amount, (1 ~ 3MPa) boiling, temperature are 100 ~ 125 ℃ to high pressure, and 2 hours time, it is stand-by to come unstuck.
3, mix.The Spine Date Seed that processing is made mixes with the collagen protein of said extracted, and wherein the ratio of weight and number of collagen protein and spiny jujuba seed powder is, 31 ~ 41: 59 ~ 61 (dry-matteies than), and concentration of substrate mixed be adjusted to 5 ~ 9%.
4, transfer pH value to 7.5 with citric acid and food grade ammoniacal liquor;
5, add compound protease, add-on is that enzymolysis is carried out in 0.8 ~ 5% (as 0.8,3 or 5) of substrate gross weight, and enzymolysis time is 4H;
6, filter, filter with flame filter press, and with filtrate through vacuum concentration, spraying drying promptly makes polypeptide of seed of spine date.
Two, polypeptide of seed of spine date sedative health-care oral preparation chewing tablet embodiment proportioning:
1, polypeptide of seed of spine date: releasing agent: seasonings=100: 1: 0.7.
2, polypeptide of seed of spine date: releasing agent: seasonings=100: 2: 1.3.
By the said ratio batching, mixing is pressed into tablet with tabletting machine, and every is 1g.With the product of the acceptance(check) packing of packing into, indicate date manufactured, factory site, seal and carry out moistureproof damp proof preservation, wait to dispatch from the factory (other preparations or food proportioning slightly, available ordinary method).
Three, the clinical test results of the health oral goods chewing tablet made from the main component polypeptide of seed of spine date (but medicine-food two-purpose).
People through 81 insomnias of certain hospital and poor sleeping quality tries clothes, and the result is as follows:
1, examination example: obtain at random.Test group 81 examples, the male sex's 52 examples wherein, women's 29 examples; Year makes 36 ~ 70 years old.Insomnia is all arranged, poor sleeping quality.
2, using method: during use the direct chewing of tablet is taken, every is 1g, each 3, sleeps and takes in preceding 1 hour.Statistics effect on the 7th.
3, mark Huaihe River: divide produce effects: insomnia disappears, and sleep quality is normal, quality good.Effectively: insomnia improves, and sleep quality has improvement.Invalid: symptom does not have improvement, no change.
4, result of use: by UR, test group: produce effects 74 examples, obvious effective rate 91%; Effective 4 examples, efficient 5%; Total effective rate 96%; Invalid 3 examples, inefficiency 4%.The result shows, health-care effect of the present invention is definite, resource is wide, method for making is simple, technical maturity, low, the easy preservation of price, and it makes the patient obtain health care under not unhealthful situation.
Four, typical case:
1, Wu * *, the man, 71 years old, on April 6th, 2006 rose and takes the polypeptide of seed of spine date chewing tablet, after 3 days, sleeping difficult symptom is obviously improved, prolong the length of one's sleep.
2, Lee * *, the man, 46 years old, on February 25th, 2006 was taken the polypeptide of seed of spine date chewing tablet, after 5 days, insomnia obviously improves, sleep quality improves.
3, the field * *, woman 36 years old took the polypeptide of seed of spine date chewing tablet on December 23rd, 2004, a week back insomnia disappears, daytime clearheaded (all the other cases slightly).
Claims (10)
1, polypeptide of seed of spine date is characterized in that it is a Spina Date Seed and collagen protein bonded polypeptide small molecule mixture soluble in water; Promoted the absorption of human body of these two kinds of materials of Spina Date Seed and collagen protein and curative effect thereof greatly and be worth, had calm and improve sleep effect, effect height, human absorptivity's height, functional component and play a role soon, take for a long time habituation not, characteristics without any side effects.
2, a kind of preparation method of polypeptide of seed of spine date according to claim 1 is characterized in that using enzymolysis process, is about to that macromolecular collagen protein mixes with spiny jujuba seed powder and enzymolysis obtains the method for small-molecular peptides.
3, the preparation method of polypeptide of seed of spine date according to claim 2 is characterized in that described enzymolysis process is: bright bone through steaming and decocting under high pressure extract collagen protein → with spiny jujuba seed powder mixing → accent pH value to 7.2 ~ 8.5 → carry out enzymolysis → filtering and concentrating.
4, the preparation method of polypeptide of seed of spine date according to claim 3 is characterized in that the temperature that collagen protein is extracted in the bright bone steaming and decocting under high pressure of ox is 100 ~ 125 ℃, pressure 1 ~ 3MPa.
5, the preparation method of polypeptide of seed of spine date according to claim 3 is characterized in that described collagen protein and spiny jujuba seed powder mixed weight portion rate are collagen protein: spiny jujuba seed powder=39 ~ 41: 59 ~ 61, and to make concentration of substrate be 5 ~ 9%.
6, the preparation method of polypeptide of seed of spine date according to claim 3 is characterized in that transferring pH value with citric acid and food grade ammoniacal liquor.
7, the preparation method of polypeptide of seed of spine date according to claim 3 is characterized in that adding compound protease and carries out enzymolysis, add-on be collagen protein mix back substrate gross weight with spiny jujuba seed powder 0.8 ~ 5%, enzymolysis time 4 ~ 5 hours.
8, according to the preparation method of claim 3,4,5,6 or 7 described polypeptide of seed of spine date, when it is characterized in that producing polypeptide of seed of spine date, filter with plate-and-frame filter press, vacuum concentration, spray-dried again.
9, the purposes of polypeptide of seed of spine date is characterized in that being used to make food, protective foods or the medicine with sedative action.
10, the purposes of polypeptide of seed of spine date according to claim 9 is characterized in that being used to make sedative health-care chewing tablet, and this chewing tablet has following components by weight percent umber proportioning, polypeptide of seed of spine date: releasing agent: transfer and hide agent=100: 1 ~ 2: 0.7 ~ 1.3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100614268A CN101003829A (en) | 2007-01-18 | 2007-01-18 | Polypeptide of seed of spine date, preparation method, and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100614268A CN101003829A (en) | 2007-01-18 | 2007-01-18 | Polypeptide of seed of spine date, preparation method, and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101003829A true CN101003829A (en) | 2007-07-25 |
Family
ID=38703206
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100614268A Pending CN101003829A (en) | 2007-01-18 | 2007-01-18 | Polypeptide of seed of spine date, preparation method, and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101003829A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174625A (en) * | 2010-12-31 | 2011-09-07 | 山东鼎力枣业食品集团有限公司 | Gold thread jujube peptide production method |
| CN110339247A (en) * | 2018-04-04 | 2019-10-18 | 南京惠宝生物医药有限公司 | A kind of preparation and application of Wild jujube seeds extract |
| CN114032267A (en) * | 2021-09-27 | 2022-02-11 | 南京中医药大学 | Preparation method and application of active peptide |
-
2007
- 2007-01-18 CN CNA2007100614268A patent/CN101003829A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174625A (en) * | 2010-12-31 | 2011-09-07 | 山东鼎力枣业食品集团有限公司 | Gold thread jujube peptide production method |
| CN102174625B (en) * | 2010-12-31 | 2013-11-06 | 山东鼎力枣业食品集团有限公司 | Gold thread jujube peptide production method |
| CN110339247A (en) * | 2018-04-04 | 2019-10-18 | 南京惠宝生物医药有限公司 | A kind of preparation and application of Wild jujube seeds extract |
| CN114032267A (en) * | 2021-09-27 | 2022-02-11 | 南京中医药大学 | Preparation method and application of active peptide |
| CN114032267B (en) * | 2021-09-27 | 2024-03-29 | 南京中医药大学 | Preparation method and application of active peptide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101244016B (en) | Application of aurantiin in preparing oral cavity nursing health products | |
| KR101272602B1 (en) | Pomegranate extract containing a high content of ellagic acid, and a method for improving effect of pomegranate extract related to postmenopausal syndrome | |
| CN104719899A (en) | Ginseng blood glucose-reducing soft sweets and preparation method | |
| US10894070B2 (en) | Drug compound for the control of blood glucose, blood lipids and weight | |
| CN103704697A (en) | Turnip-fructus hippophae tablet and preparation method and applications thereof | |
| CN105707887A (en) | Health care and weight loss chewable tablet and preparation method thereof | |
| CN101167898A (en) | Traditional Chinese medicine preparation for treating insomnia | |
| CN104352624B (en) | Application of the anaesthetic core fragrant plant n-butanol extract in preventing and treating diabetes medicament is prepared | |
| US20020076451A1 (en) | Health food products | |
| CN107319242A (en) | L-cn effervescent tablet for slimming and preparation method thereof | |
| CN101003829A (en) | Polypeptide of seed of spine date, preparation method, and application | |
| RU2351354C1 (en) | Medication for treatment and prevention of thyroid gland diseases | |
| CN1329049C (en) | Blood sugar- and blood pressure-lowering medicine | |
| CN103039985A (en) | Health food with function of physical fatigue relief function | |
| RU2333764C1 (en) | Medicine for treatment and prevention of thyroid gland diseases | |
| KR101910013B1 (en) | A composition for improving, preventing and treating of pain comprising herb extract | |
| JP4371431B2 (en) | Antiallergic composition | |
| CN101002932A (en) | Tuckahoe polypeptide, its preparing method and use | |
| CN101816708B (en) | Chinese medicinal composition for curing rheumatism and preparation method thereof | |
| RU2472357C1 (en) | Biologically active food additive with immunostimulating and strengthening effect | |
| RU2182011C1 (en) | Biologically active food additive to prevent reduction of immune system | |
| JP4818549B2 (en) | Aldose reductase inhibitor | |
| CN1321630C (en) | Method for preparing blood flow cutting off dispersing tablet | |
| CN102178873B (en) | Chinese medicine weight-losing preparation comprising external preparation and internal preparation and preparation method thereof | |
| CN101002933A (en) | Honeysuckle polypeptide, its preparing method and use |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |