CN100589818C - Immunopotentiator of trepang compound Chinese traditional medicine - Google Patents

Abstract

The invention discloses a compound Chinese herbal immunopotentiator which comprises the following constituents (by weight percent): schisandra fruit 15-25%, root of herbaceous peony 5-15%, cinnamon twig 5-15%, perilla fruit 15-35%, poria cocos wolf 15-25% and polyporus umbellatus 15-25%.

Description

Trepang compound Chinese herbal medicine immunostimulant

Technical field:

The present invention relates to a kind of compound Chinese herb immunostimulant, especially a kind of nontoxic, having no side effect obviously to strengthen the Chinese medicine formula of Stichopus japonicus immunocompetence.

Background technology:

After the pool cultivated outburst of the coastal shrimp nineties on a large scale can not cure diseases, apostichopus japonicus culture replaces prawn culturing gradually became the main project that sea pond is cultured, because of the economic benefit of culturing Radix Morinae Bulleyanae considerable, so apostichopus japonicus culture is just in cumulative year after year.But, the deterioration of, carrying capacity excessive overload and breeding environment along with holothruian cultures density, the Stichopus japonicus disease is outburst again and again also, the common self-dissolving (being commonly called as " change plate ") that has the mashed limit of auricularia in seedling stage, skin ulcer to cause, the skin fester disease that the mature stage Stichopus japonicus occurs, swollen mouth disease or the like.At present, the Stichopus japonicus prevention and treatment of diseases mostly adopts the antibiotic dipping to handle, and especially the growing seedlings and indoor keeping a full stand of seedings the stage of Stichopus japonicus, the Drug resistance of antibacterial, virus strengthens gradually, to such an extent as to the essential dosage that strengthens.The excessive antibiotic and the use of chemical drugs cause the intravital antibiotic remains of Stichopus japonicus to exceed standard, and not only influence ingesting and growing of Stichopus japonicus, have also influenced the marketing quality of Radix Morinae Bulleyanae simultaneously, and people's edible safety is also constituted a serious threat; Water body has also been polluted in the use of other chemical classes disinfectant, causes environmental pollution.

Summary of the invention:

The present invention is in order to solve the existing the problems referred to above of prior art, a kind of characteristics such as natural sex, multifunctionality, noresidue, no Drug resistance, price is low, the source is abundant that have are provided, can improve the immunity of Stichopus japonicus, improve the trepang compound Chinese herbal medicine immunostimulant of disease-resistant, the anti-adverse environment ability of seed.

Technical solution of the present invention is: a kind of trepang compound Chinese herbal medicine immunostimulant is characterized in that each weight percentages of components is as follows:

Fructus Schisandrae Chinensis 15%～25%

The Radix Paeoniae Alba 5%～15%

Ramulus Cinnamomi 5%～15%

Folium Perillae 15%～35%

Poria 15%～25%

Polyporus 15%～25%.

Each component optimum weight percentage ratio is as follows:

Fructus Schisandrae Chinensis 20%

The Radix Paeoniae Alba 10%

Ramulus Cinnamomi 10%

Folium Perillae 20%

Poria 20%

Polyporus 20%.

Fructus Schisandrae Chinensis is the dry mature fruit of magnoliaceae schisandra, has another name called: face rattan, Fructus Zanthoxyli Plansipini.Contain deoxyschizandrin, deoxyschisandrin, schisandrol, schizantherin etc.; the syncytium that deoxyschizandrin can suppress virus induction forms; the cell of protection viral infection suppresses virus and duplicates intracellular, and is inhibited in early days to the HIV-1 virus replication.These flavour of a drug are acid warm, and the effect of supplementing QI for promoting the production of body fluid is arranged.

Poria is the sclerotium of plant Polyporaceae Poria, its main component is pachyman, pachymic acid etc., after digestion, be transformed into pachyman and have the enhancing human body immunity function, promote cell division, activating complement, effects such as antimutagenic are to the effect of being significantly improved of the cytophagous phagocytic activity of mice body cavity.

The Radix Paeoniae Alba is rich in paeoniflorin, the water decoction of the Radix Paeoniae Alba has obvious facilitation to the phagocytic activity of macrophage, dysentery bacterium there is bacteriostasis, in addition, can also suppresses staphylococcus, the alcohol agent can suppress bacillus pyocyaneus, Radix Paeoniae Alba preserved material also has inhibitory action to some pathogenic fungus, to pyococcus, and gastrointestinal pathogen and conditioned pathogen, as escherichia coli, Streptococcus viridans etc., antibacterial action is in various degree arranged all.

The hot temperature of Ramulus Cinnamomi, it is cold to be apt to dispel the wind, can cure cold wind and cold, fever with chills, Stichopus japonicus water body of living in belongs to the ground of YIN-cold, and this medicine and Poria collocation are used and can be changed YIN-cold, defeated body fluid; Ramulus Cinnamomi leachate filter paper is studied staphylococcus aureus, Staphylococcus albus, bacillus pyocyaneus, Bacillus proteus, alpha streptococcus, group B streptococcus bacteriostasis, and the result shows all has obvious bacteriostasis to above antibacterial.

The hot temperature of Folium Perillae, have deliver, cold expelling, the effect of regulating the flow of vital energy.It is about 0.5% that the Folium Perillae herb contains volatile oil, includes perillaldehyde etc., can strengthen the reticuloendothelial system function, improves immunity.

The present invention be according to the formulation principle of Chinese herbal medicine when strengthening drug effect, to not damage of Stichopus japonicus, can in the Stichopus japonicus body, not produce residually, can not influence the normal growth of Stichopus japonicus, quite remarkable to the raising effect of Stichopus japonicus immunity.When regulating and improving Stichopus japonicus immunity, can also play antibacterial, germ-resistant effect, avoided using the weak point of antibiotics and chemical disinfection quasi drugs, have no side effect, non-corrosiveness, safe in utilization, degradable under field conditions (factors), also pollution-free to water environment, be the product that effectively prevents the Stichopus japonicus disease.Reasonable compatibility of the present invention makes some itself contain the perform toxic attenuation or the elimination of the natural goods Chinese herbal medicine of toxic component, brings into play its useful effect better.Component steady sources of the present invention, with low cost, low price, production technology simple possible.

Description of drawings:

Fig. 1 is the embodiment of the invention is organized the influence of alkaline phosphatase activities to the Stichopus japonicus body wall a sketch map.

The specific embodiment:

Embodiment 1:

Get 15～25 gram Fructus Schisandrae Chinensis, 10～15 gram Radix Paeoniae Albas, 15～15 gram Ramulus Cinnamomi, 15～25 gram Folium Perillaes, 15～25 Poria, 15～25 gram Polyporus, get every flavor medicine and can in described scope, choose, but weight should be 100 grams.Pulverized 200 order mesh screens, the dosage with feedstuff weight 1% behind the medicated powder mixing adds in the Stichopus japonicus normal feedstuff, and water and even looses and throws in Miao Chizhong before throwing something and feeding.

Embodiment 2:

20 gram Fructus Schisandrae Chinensis, the 10 gram Radix Paeoniae Albas, 10 gram Ramulus Cinnamomi, 20 gram Folium Perillaes, 20 gram Poria, 20 gram Polyporus, pulverized 200 order mesh screens, the dosage with feedstuff weight 2% behind the medicated powder mixing adds in the Stichopus japonicus normal feedstuff, and attention must mix homogeneously, water is transferred and is mixed slurry before throwing something and feeding, and looses and throws in Miao Chizhong.

Embodiment 3:

25 gram Fructus Schisandrae Chinensis, the 5 gram Radix Paeoniae Albas, 15 gram Ramulus Cinnamomi, 25 gram Folium Perillaes, 15 gram Poria, 15 gram Polyporus, pulverized 200 order mesh screens, the dosage with feedstuff weight 3% behind the medicated powder mixing adds in the Stichopus japonicus normal feedstuff, and attention must mix homogeneously, water is transferred and is mixed slurry before throwing something and feeding, and looses and throws in Miao Chizhong.

Result of use is as follows:

1. see Table 1 with present embodiment 1,2,3 influences to the Stichopus japonicus growth in back 30 days

Table 1

	Matched group	Embodiment 1	Embodiment 2	Embodiment 3
					Average weight gain amount (g)	2.05	2.55	2.58	2.4
Average growth rate (%)	48.69％	49.70％	55.48％	48.78％

Matched group wherein is the Stichopus japonicus group that does not apply the Chinese herbal medicine feeding.The average weight gain of this utility model embodiment 1,2,3 all is higher than matched group, embodiment 1, embodiment 3 processed group average growth rates and matched group approaching, and embodiment 2 average growth rates are higher than matched group.

2. see Table 2 with present embodiment 1,2,3 back phagocyte percentage compositions (* 100%)

Table 2

The sampling number of times	Matched group	Embodiment 1	Embodiment 2	Embodiment 3
					For the first time	0.109±0.021	0.237±0.016	0.383±0.012	0.279±0.009
For the second time	0.109±0.022	0.274±0.012	0.309±0.006	0.355±0.034
					For the third time	0.109±0.023	0.154±0.005	0.189±0.009	0.376±0.008
The 4th time	0.109±0.024	0.268±0.01	0.288±0.033	0.304±0.021

As shown in table 2, handle with the embodiment of the invention 1,2,3 and to make that cytophagous percentage composition increases to some extent in the Stichopus japonicus coelomocyte, embodiment 2 and 3 groups of performances are more outstanding, 3 groups of prolongations of embodiment with the time of throwing something and feeding, and the phagocyte percentage composition maintains more than 30% substantially.

3. see Table 3 with present embodiment 1,2,3 posterior coelom liquid phagocyte phagocytic rates (* 100%)

Table 3

As shown in table 3: as to handle with the embodiment of the invention 1,2,3, the cytophagous phagocytic rate of Stichopus japonicus body cavity is obviously improved, than matched group, the phagocytic rate of embodiment 2 and embodiment 3 improves near one times, two groups have all reached more than 50% when taking a sample for the second time, and effect is very stable in throwing something and feeding for a long time, all is higher than matched group.

4. see Table 4 with present embodiment 1,2,3 posterior coelom liquid phagocyte phagocytic index

Table 4

5. see Table 5 with present embodiment 1,2,3 posterior coelom coelomic fluid activity of lysozyme (U/ml)

Table 5

The sampling number of times	Matched group	1% group	2% group	3% group
					For the first time	77.630	96.432	118.298	100.471
For the second time	77.630	85.290	155.901	70.109
					For the third time	77.630	92.950	113.144	134.314
The 4th time	77.630	131.389	153.533	104.370

As shown in table 3: make Stichopus japonicus coelomic fluid activity of lysozyme obviously improve with the embodiment of the invention 1,2,3 processing, the raising effect of embodiment is the most outstanding.

1,2,3 pairs of body walls of embodiment organize alkaline phosphatase activities influence as shown in Figure 1: alkali phosphatase is the lysosomal marker enzyme of Stichopus japonicus, the raising of the raising explanation Stichopus japonicus lysosome content of its content, promptly the defence capability to exogenous invader improves, the embodiment of the invention 1,2,3 has improved body wall and has organized alkaline phosphatase activity, and with the prolongation of the time of throwing something and feeding, alkaline phosphatase activity is strong more.

Claims (2)

1. trepang compound Chinese herbal medicine immunostimulant is characterized in that each weight percentages of components is as follows:

Fructus Schisandrae Chinensis 15%～25%

The Radix Paeoniae Alba 5%～15%

Ramulus Cinnamomi 5%～15%

Folium Perillae 15%～35%

Poria 15%～25%

Polyporus 15%～25%.

2. trepang compound Chinese herbal medicine immunostimulant according to claim 1 is characterized in that each weight percentages of components is as follows:

Fructus Schisandrae Chinensis 20%

The Radix Paeoniae Alba 10%

Ramulus Cinnamomi 10%

Folium Perillae 20%

Poria 20%

Polyporus 20%.

Images