CN100563446C - A kind of biologic product that promotes tomato plant strain growth, control tomato late blight and preparation method thereof and application - Google Patents
A kind of biologic product that promotes tomato plant strain growth, control tomato late blight and preparation method thereof and application Download PDFInfo
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- CN100563446C CN100563446C CNB2008100581502A CN200810058150A CN100563446C CN 100563446 C CN100563446 C CN 100563446C CN B2008100581502 A CNB2008100581502 A CN B2008100581502A CN 200810058150 A CN200810058150 A CN 200810058150A CN 100563446 C CN100563446 C CN 100563446C
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Abstract
The invention provides a kind of biologic product that promotes tomato plant strain growth, control tomato late blight and preparation method thereof and application, relate to biological pesticide technical field.Biologic product of the present invention is by giving birth to Ovum Anas domestica yolk 50 grams, 100 milliliters of edible vegetable oils, 100 milliliters in clear water, 62.5 milliliters of compositions of the bacillus thuringiensis,Bt of 1000 international units/microlitre.Biologic product preparation process of the present invention is the mixed process to above-mentioned four kinds of compositions, easy to make, economical and effective, and cost is low, safety non-pollution.Biologic product of the present invention on average improves 32.72～36.03% to the control efficiency of tomato late blight than conventional chemical medicament, improves 129.35～434.57% than bacillus thuringiensis,Bt.With the clear water contrast, the rate that increases of plant is 6.19～14.19%.
Description
Technical field
The present invention relates to biological pesticide technical field.Be specifically related to a kind of biologic product that promotes tomato plant strain growth, control tomato late blight and preparation method thereof and application.
Background technology
Chemical pesticide is extensive and application blindly in agricultural production, and the agricultural ecological balance is wrecked, and environment is subjected to severe contamination, injures people and animals' health simultaneously.21 century is ecological century of an environmental protection; along with the day by day attention of the mankind to environmental and ecological protection; in agricultural production, adopt, noresidue, non-killed natural enemies and beneficial organism nontoxic, free from environmental pollution, be fit to the biological control of nuisanceless production requirement the person poultry safety; greatly develop the requirement that biopesticide has adapted to the epoch, had very important meaning.
Though biopesticide has obtained wider exploitation and has used widely, but also there is the R﹠D costs height in biopesticide at aspects such as research and development, production and application, drug effect is slow, the term of validity is short, relatively the insecticidal and antibacterial spectrum is narrow and in a short time to problem and difficulty such as a little less than the Pest management power, become defective of the prior art and restricted the development of biopesticide, to overcome on the aforementioned defective of the prior art basis, develop and to improve the emphasis that biopesticide that the control of crop disease effect can better promote plant growth again becomes the present invention's research.
Summary of the invention
The present invention has developed and can improve the biologic product that the control of crop disease effect can better promote plant growth again in order to overcome the deficiency of above-mentioned biopesticide in producing, using.
Drug effect, the drug effect term of validity that biologic product of the present invention has improved biologic product greatly are longer, with strong points to tomato late blight control.Biologic product of the present invention has on average improved 32.72～36.03% to the control efficiency of tomato late blight than conventional chemical medicament, has improved 129.35～434.57% than bacillus thuringiensis,Bt.Biologic product of the present invention is to tomato plant strain growth, compare with the clear water contrast, plant increased rate 6.19～14.19%.
The present invention screens a kind of biologic product that promotes tomato plant strain growth, control tomato late blight from development test repeatedly, this biologic product has that preparation technology is simple, research cost is low, free from environmental pollution, to advantages such as person poultry safeties.
Technical scheme of the present invention is:
One, a kind of biologic product that promotes tomato plant strain growth, control tomato late blight, this biologic product is formed by following component and content:
Give birth to Ovum Anas domestica yolk: 50 grams
Edible vegetable oil: 100 milliliters
Clear water: 100 milliliters
The bacillus thuringiensis,Bt of 1000 international units/microlitre: 62.5 milliliters (suspending agent)
Above-mentioned living Ovum Anas domestica yolk is fresh living Ovum Anas domestica yolk, and above-mentioned edible vegetable oil is commercially available edible vegetable oil, preferred rapeseed oil.
Two, the preparation method of above-mentioned biologic product, step is in the following order carried out:
(1) takes out living Ovum Anas domestica yolk 50 grams, add 100 milliliters of edible vegetable oils, full and uniform stirring;
(2) add 100 milliliters of clear water, white emulsion is made in full and uniform stirring;
(3) 62.5 milliliters of the bacillus thuringiensis,Bts (suspending agent) of adding 1000 international units/microlitre, fully mixing is stored under 5 ℃ of conditions, and is standby.
Three, the application of above-mentioned biologic product, its application are the application at the control tomato late blight.Percent by volume with above-mentioned biologic product and clear water is 1: 100, and every square metre is used biologic product amount of the present invention is 0.75 milliliter, and compound method is a conventional method.With the whole vegetative growth phase of spray-on process,, used once in per 7 days all even and fine spraying of complete stool leave dual sides tomato.
Four, the application of above-mentioned biologic product, its application are in the application that promotes tomato plant strain growth.Percent by volume with above-mentioned biologic product and clear water is 1: 100, and every square metre is used biologic product amount of the present invention is 0.75 milliliter, and compound method is a conventional method.With the whole vegetative growth phase of spray-on process,, used once in per 7 days all even and fine spraying of complete stool leave dual sides tomato.
Embodiment:
Embodiment:
One, experimental condition
1, trial crops
Tomato: solanaceous crops (kind is a diamond)
2, environmental condition
Experimental field be positioned at winter vegetable main producing region, Yunnan Province Yuanmou County, this county soil is the black loam, alkalescent, and middle fertility, peasant's growing vegetables technical merit are better, and cultivation management standard, irrigation conditions are better.
Two, experimental scheme and arrangement
1, test medicine
Test medicine is a biologic product of the present invention, and its component and content are as follows:
Give birth to Ovum Anas domestica yolk: 50 grams
Rapeseed oil: 100 milliliters
Clear water: 100 milliliters
The bacillus thuringiensis,Bt of 1000 international units/microlitre (suspending agent): 62.5 milliliters.
This bacillus thuringiensis,Bt is by the development of Hubei bacillus thuringiensis,Bt research and development centre, and Kang Xin agricultural pharmaceutcal corporation, Ltd in Hubei produces.
Above-mentioned living Ovum Anas domestica yolk is fresh living Ovum Anas domestica yolk, and above-mentioned rapeseed oil is commercially available rapeseed oil.
The preparation method of above-mentioned biologic product as previously mentioned.
Test medicine is 1: 100 by the percent by volume of biologic product and clear water, and every square metre is used investigational agent dosage is 0.75 milliliter, and its compound method is a conventional method.
2, contrast medicament
(1), the bacillus thuringiensis,Bt (hereinafter to be referred as Bt) of 1000 international units/microlitre begins dispenser when the crop transplant survival, dispenser in per 7 days once, the pharmacy application amount is by the requirement of package insert.
(2), conventional chemical medicament: commercially available fast clean, streptomycin, S-Ethyl ethylthio sulfonate, thickness, eugenol, product promise, Jin Lei, torch, Cisco, the rice acid amides manganese salt etc. of pressing down.According to the conventional method of application of peasant in the land for growing field crops, whole vegetative growth phase of tomato according to crop disease and insect a situation arises dispenser in good time, the chemical agent type is to select the various chemical medicaments mixing of aforementioned conventional chemical medicament and intersect to use, and the pharmacy application amount is by the requirement of package insert.
3, sub-district area and repetition
Small plot experiment adopts randomized arrangement.4 processing are established in test, and each is handled 3 times and repeats, and each repeats 50m2.
Handle 1: biologic product; Handle 2: the conventional chemical medicament; Handle 3: bacillus thuringiensis,Bt; Handle 4: the clear water contrast.
4, application method
Adopt the spraying of WS-16 type (Shandong Wish Plant Protection Machinery Co., Ltd.) knapsack hand sprayer.To complete stool leave dual sides even spraying.
5, spraying time
The bacillus thuringiensis,Bt of biologic product of the present invention and contrast medicament 1000 international units/microlitre begins dispenser when the crop transplant survival, dispenser in per 7 days once.The contrast chemical agent is according to crop disease and insect a situation arises dispenser in good time.
6, meteorology and soil information
22 ℃ of duration of test mean temperature of air, 27 ℃ of maximum temperatures, 10 ℃ of minimum temperatures.Experimental field physical features is smooth, and soil is the black loam, alkalescent, middle fertility.
Three, investigation method, time and number of times
Whenever after executing 3 medicines investigation in 7 days once, 5 sampling surveys in each experimental plot, every some investigation 3 strains, a situation arises for the disease of each 4 leaf of investigation plant height and every strain upper, middle and lower.Grade scale according to tomato late blight carries out classification, calculates disease index disease, and adopts the DMRT method that the result is carried out statistical analysis.
Four, result and analysis
The embodiment data see table 1, table 2, table 3 for details.
Find out vegetative growth phase tomato by three investigation results of table 1, table 2, biologic product of the present invention on average improves 32.72～36.03% to the control efficiency of tomato late blight than conventional chemical medicament, improves 129.35～434.57% than bacillus thuringiensis,Bt.Found out that by the result of the test of table 3 tomato plant of using biologic product of the present invention compares with the clear water contrast, the rate that increases of plant is 6.19～14.19%.
Result of the test shows that this biologic product can effectively prevent and treat tomato late blight, promote tomato plant strain growth.Biologic product of the present invention in agricultural production, especially will play important as the biologic product of preventing and treating tomato late blight, promoting tomato plant strain growth in green A level pollution-free vegetable plantation
The investigation result of table 1. tomato late blight different times
Table 2. biologic product of the present invention and conventional chemical medicament, bacillus thuringiensis,Bt
Average control efficiency to tomato late blight compares
The plant height investigation result of table 3. tomato different times
Claims (9)
1, a kind of biologic product that promotes tomato plant strain growth, control tomato late blight, it is characterized in that: this biologic product is formed by following component and content,
Give birth to Ovum Anas domestica yolk: 50 grams
Edible vegetable oil: 100 milliliters
Clear water: 100 milliliters
The bacillus thuringiensis,Bt of 1000 international units/microlitre: 62.5 milliliters.
2, the described biologic product of claim 1, it is characterized in that: described edible vegetable oil is rapeseed oil.
3, the preparation method of claim 1 or the described biologic product of claim 2, it is characterized in that: step is in the following order carried out:
(1) takes out living Ovum Anas domestica yolk 50 grams, add 100 milliliters of edible vegetable oils, full and uniform stirring;
(2) add 100 milliliters of clear water, milky emulsion is made in full and uniform stirring;
(3) bacillus thuringiensis,Bt of adding 1000 international units/microlitre is 62.5 milliliters, and fully mixing is stored under 5 ℃ of conditions, and is standby.
4, the application of the biologic product of claim 1 or claim 2 is characterized in that: the application of control tomato late blight.
5, the application of the described biologic product of claim 4 is characterized in that: the percent by volume of described biologic product and clear water is 1: 100, and every square metre is used described biologic product amount is 0.75 milliliter.
6, the application of the described biologic product of claim 5: it is characterized in that: adopt spray-on process to use the whole vegetative growth phase tomato, to complete stool leave dual sides even spraying, used once in per 7 days.
7, the application of the biologic product of claim 1 or claim 2 is characterized in that: in the application that promotes tomato plant strain growth.
8, the application of the described biologic product of claim 7 is characterized in that: the percent by volume of described biologic product and clear water is 1: 100, and every square metre is used described biologic product amount is 0.75 milliliter.
9, the application of the described biologic product of claim 8: adopt spray-on process to use the whole vegetative growth phase tomato, to complete stool leave dual sides even spraying, used once in per 7 days.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2008100581502A CN100563446C (en) | 2008-03-05 | 2008-03-05 | A kind of biologic product that promotes tomato plant strain growth, control tomato late blight and preparation method thereof and application |
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| CNB2008100581502A CN100563446C (en) | 2008-03-05 | 2008-03-05 | A kind of biologic product that promotes tomato plant strain growth, control tomato late blight and preparation method thereof and application |
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| CN101258858A CN101258858A (en) | 2008-09-10 |
| CN100563446C true CN100563446C (en) | 2009-12-02 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2010079762A1 (en) * | 2009-01-09 | 2010-07-15 | 雪印種苗株式会社 | Production method for phenyl alkyl succinic acid amide compounds |
| CN104068067B (en) * | 2014-06-20 | 2017-01-25 | 浙江省农业科学院 | Application of yolk and edible oil mixture to production of reagent for preventing and treating crop diseases and pests |
| CN107306946B (en) * | 2017-05-10 | 2021-02-12 | 中国农业大学 | Egg yolk oil pesticide and preparation method and application thereof |
| CN109430293B (en) * | 2018-10-30 | 2020-11-13 | 宁夏大学 | Pharmaceutical composition for preventing and treating tomato blight and promoting tomato growth, and preparation method, application method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5362707A (en) * | 1990-12-10 | 1994-11-08 | Rhone-Poulenc Chimie | Plant-protection suspo-emulsions comprising sucroglycerides |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5362707A (en) * | 1990-12-10 | 1994-11-08 | Rhone-Poulenc Chimie | Plant-protection suspo-emulsions comprising sucroglycerides |
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