CN100546491C - Animal fermented concentrated feed and mixed feed and preparation method thereof and equipment - Google Patents
Animal fermented concentrated feed and mixed feed and preparation method thereof and equipment Download PDFInfo
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- CN100546491C CN100546491C CNB2006100023899A CN200610002389A CN100546491C CN 100546491 C CN100546491 C CN 100546491C CN B2006100023899 A CNB2006100023899 A CN B2006100023899A CN 200610002389 A CN200610002389 A CN 200610002389A CN 100546491 C CN100546491 C CN 100546491C
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
Abstract
A kind of preparation method of animal fermented concentrated feed: its component and percentage by weight thereof consist of: dregs of beans 30-50%, peanut meal 5-20%, corn protein powder 20-40%, calcium monohydrogen phosphate 5-10%, salt 2-3%, micro-0.1-0.2%, leavening 0.1-0.2%; The step of preparation: 1. bacterial classification brings back to life to expand and grows the making strain liquid; 2. after strain liquid and dregs of beans, peanut meal, corn protein powder, calcium monohydrogen phosphate, salt, each component of trace element being mixed, stir and the interior heat sealing of the fermentation packaging bag of packing into; 3.30-15 can use after it fermentation.The preparation method of animal fermented mixed feed is provided in addition, and the making apparatus of above-mentioned feed and fermentation packaging bag.Above-mentioned feed has promoted lactic acid bacteria and saccharomycete and various enzyme, crude protein, amino acid etc., and the enzyme that microbial metabolism produces is also very high, is antibiotic-free feed, and feeding animals is effective.
Description
Technical field
The present invention relates to the preparation method and the equipment of feed, relate in particular to a kind of animal fermented concentrated feed and mixed feed and preparation method thereof and equipment.
Background technology
From the seventies, China introduces advanced cultural technique successively and carries out large-scale farming.Quantity is cultured by a unit, develops into several thousand, several ten thousand from tens.For example raise chickens and only develop into several thousand, 1,000,000 from tens, hundreds of.Many pig houses are tightly continuous, and chicken coop one by one.Under such environment, air dirt in pig house, the hen house, dust from flying, ight soil overflows, and the pig chicken is easy to sick, and pestilence is also more and more general, and if do not take technical measures, pig, chicken can't survive at all.For this reason, use antibiotic prophylaxis pig, the sick measure of thinking that aquaculture must be taked of chicken in feed, antibiotic addition and kind also increase gradually and upgrade in feed, also do not know what to have added in the feed till now, what have added, and the medicine inspection expert also can hardly be explained sometimes.
Antibiotic abuse makes virus, livestock and poultry produce the resistance to the action of a drug and medicament residue, by food chain the mankind has been caused serious harm.Therefore, the use of restriction antibiotic in animal and fowl fodder, studying green antibiotic-free feed is the important research direction of recent countries in the world feed industry.
In animal feed was used, fermentative feedstuff of microbe report at home and abroad was a lot, but because its production cost is very high, maybe can't circulate, or quality of production instability, caused result of use bad, thereby failed in actual use to popularize.Why unsuccessful the traditional zymotic feed is, and main cause is not have workout cost and result of use problem.Can fermentative feedstuff of microbe feasible, do not lie on the conversion technology whether feasible, and be whether feasible economically, accomplish viable economically just must comprehensive nutrition, with low cost.
Prerequisite with low cost must be to use effective, and is just with low cost, and result of use is bad also to be frustrated.The reason that fermented feed is better than normal diet result of use is, a large amount of lactic acid bacterias and saccharomycete and various organized enzyme are arranged in the fermented feed, if in normal diet, add lactic acid bacteria and saccharomycete and various organized enzyme, also can improve the result of use of normal diet, but cost will increase substantially certainly.Therefore obtaining a large amount of lactic acid bacterias, saccharomycete and various organized enzyme with the method for fermentation, is exactly the most effective means.But bacterial strain has aerobic, and anaerobism is arranged, and generally, these two kinds of microorganisms are incompatible, and fermented feed does not in the past address this problem.
Summary of the invention
The purpose of this invention is to provide a kind of animal fermented concentrated feed and mixed feed and preparation method thereof and equipment.Adopting fermentative feedstuff of microbe biotechnology of the present invention is the important channel of realizing this order ground, the feed that this technology is produced has growth promotion, have no side effect, antibiotic-free is residual, pollution-free, the no resistance to the action of a drug can improve the not available advantages of normal diet such as efficiency of feed utilization and enjoys attention.
For reaching this purpose, the preparation method of a kind of animal fermented concentrated feed provided by the invention, the step of this method is as follows:
One, preparation bacterial strain:
(1) healthy live hog is butchered, got large intestine, small intestine, stomach, interior thing and mucous membrane under the aseptic condition, add the sterilized water dilute filtration and take out 50 milliliters;
(2) adopt quartering progressively to taper to 1 milliliter, add the sterilized water dilution, be mixed with concentration and be 102,103,104,105,106 dilution;
(3) dilution is uniformly coated on respectively on a plurality of solid potato agar culture mediums, each is coated with 0.5ml;
(4) through 28-30 ℃ of constant temperature culture 5 days, clump count was no more than single bacterium colony of 10 as the preliminary election bacterial strain.
Two, preparation leavening:
(1) the purebred nutrient solution that spreads cultivation of preparation: the purebred nutrient solution that spreads cultivation is by containing sucrose 40g, glucose 40g, bean cake powder 40g, corn flour 40g, potassium dihydrogen phosphate 3g composition among every 1000g, nutrient solution was 120 ℃ of high-temperature sterilizations 40 minutes, be cooled to after 35 ℃, by 0.1% weight ratio inoculating strain;
(2) purebred spreading cultivation: lactic acid bacteria 34-36 ℃ constant temperature culture 8 days, viable lactic acid bacteria quantity 1 * 108/gram more than.Saccharomycete is 34-36 ℃ of constant temperature culture 6 days, the saccharomycete number of viable 106/more than the gram, obtain the leavening bacterial classification after spreading cultivation, its Ph is about 5;
(3) preparation bacterial classification growth accelerator: the component and the percentage by weight thereof of bacterial classification growth accelerator consist of:
FOS 30-50%,
Lactic acid 20-40%,
Ammonium hydrogen phosphate 5-10%,
The plain 5-20% of growth of microorganism;
(4) preparation leavening:
Get bacterial classification 100 gram after spreading cultivation, get bacterial classification growth accelerator 900 grams and add up to 1000 gram packings to make leavening.
Three. the preparation animal fermented concentrated feed:
1) component of animal fermented concentrated feed and percentage by weight thereof consist of:
Dregs of beans 30-50%,
Peanut meal 5-20%,
Corn protein powder 20-40%,
Calcium monohydrogen phosphate 5-10%,
Salt 2-3%,
Trace element 0.1-0.2%,
Leavening 0.1-0.2%;
2) step of preparation animal fermented concentrated feed:
(1) leavening is put into 30 ℃ of milk and stirred, placed then 15 minutes, carry out pouring in the warm water as strain liquid after bacterial classification resurrection expansion is grown;
(2) above-mentioned strain liquid and dregs of beans, peanut meal, corn protein powder, calcium monohydrogen phosphate, salt, each component of trace element are mixed after, pour in the mixer and stir, open the mixer bottom door material put into duplex conveyor, and by duplex conveyor with the material heat sealing in the fermentation packaging bag of packing into;
(3) in room temperature 10-40 ℃ scope after 30-15 days the fermentation, animal fermented concentrated feed can use.
The preparation method of animal fermented concentrated feed provided by the invention, wherein said bacterial strain is one or more in Bacillus acidi lactici, streptococcus fecalis, bacillus, saccharomycete, Trichoderma viride and the aspergillus niger.
For reaching aforementioned this purpose, the preparation method of a kind of animal fermented mixed feed of the present invention, the step of this method is as follows:
One, preparation bacterial strain:
(1) healthy live hog is butchered, got thing and mucous membrane in large intestine, small intestine, the stomach under the aseptic condition, add the sterilized water dilute filtration and take out 50 milliliters;
(2) adopt quartering progressively to taper to 1 milliliter, add the sterilized water dilution, being mixed with concentration is 10
2, 10
3, 10
4, 10
5, 10
6Dilution;
(3) described dilution is uniformly coated on respectively on a plurality of solid potato agar culture mediums, each is coated with 0.5ml;
(4) through 28-30 ℃ of constant temperature culture 5 days, clump count was no more than single bacterium colony of 10 as the preliminary election bacterial strain;
Two, preparation leavening:
(1) the purebred nutrient solution that spreads cultivation of preparation: the purebred nutrient solution that spreads cultivation is by containing sucrose 40g, glucose 40g, bean cake powder 40g, corn flour 40g, potassium dihydrogen phosphate 3g preparation among every 1000g, nutrient solution was 120 ℃ of high-temperature sterilizations 40 minutes, be cooled to after 35 ℃, by 0.1% weight ratio inoculating strain;
(2) purebred spreading cultivation: lactic acid bacteria 34-36 ℃ constant temperature culture 8 days, viable lactic acid bacteria quantity is 1 * 10
8Individual/more than the gram; Saccharomycete was 34-36 ℃ of constant temperature culture 6 days, and the saccharomycete number of viable is 10
6Individual/as more than the gram, to obtain the leavening bacterial classification after spreading cultivation, its Ph is about 5;
(3) preparation bacterial classification growth accelerator: the component and the percentage by weight thereof of bacterial classification growth accelerator consist of:
FOS 30-50%,
Lactic acid 20-40%,
Ammonium hydrogen phosphate 5-10%,
The plain 5-20% of growth of microorganism;
(4) preparation leavening: get bacterial classification 100 gram after spreading cultivation, get bacterial classification growth accelerator 900 grams and add up to 1000 grams to be packaged into leavening;
Three. prepare animal fermented mixed feed:
1) component of animal fermented mixed feed and percentage by weight thereof consist of:
Corn 20-30%,
Dregs of beans 20-30%,
Whey powder 5-10%,
Peanut meal 5-10%,
Fish meal 2-5%,
Vegetable oil 2-5%,
Brown sugar 2-5%,
Corn protein powder 5-15%,
Calcium monohydrogen phosphate 2-3%,
Salt 0.2-0.5%,
Trace element 0.1-0.2%,
Leavening 0.1-0.2%;
2) step of the animal fermented mixed feed of preparation:
(1) leavening is put into 30 ℃ of milk and stirred, placed then 15 minutes, carry out pouring in the warm water as strain liquid after bacterial classification resurrection expansion is grown;
(2) above-mentioned strain liquid and corn, dregs of beans, whey powder, peanut meal, fish meal, vegetable oil, brown sugar, corn protein powder, calcium monohydrogen phosphate, salt, each component of trace element are mixed after, pour in the mixer and stir, open the mixer bottom door material is put into duplex conveyor, and the material fermentation packaging bag of packing into is carried out heat seal encapsulation by duplex conveyor;
(3) in room temperature 10-40 ℃ scope after 30-7 days the fermentation, animal fermented mixed feed can use.
The preparation method of animal fermented mixed feed provided by the invention, wherein said bacterial strain is one or more in Bacillus acidi lactici, streptococcus fecalis, bacillus, saccharomycete, Trichoderma viride and the aspergillus niger.
For reaching aforementioned this purpose, the invention provides a kind of duplex conveyor for preparing animal fermented concentrated feed or mixed feed, comprise top box and be arranged in first of top box and cut with scissors cage and drive motors thereof, described top box below is provided with the hopper that is fixed together with described top box, the bottom of described hopper is provided with second hinge cage and the drive motors thereof, and the below of described hopper is provided with discharging opening.
Duplex conveyor provided by the invention, wherein said hopper and the described second hinge cage are provided with 2 covers; Described top box, hopper, the first hinge cage and the second hinge cage adopt stainless steel to make.
Duplex conveyor provided by the invention, the bottom of wherein said top box is equipped with dodge gate and drive motors thereof.
For reaching aforementioned this purpose, the invention provides a kind of fermentation packaging bag that is used to prepare animal fermented concentrated feed or mixed feed, comprise the tube bag, described tube bag one end is provided with heat sealing, its bag is provided with at least one through hole, be coated with breather valve on the described through hole, described breather valve and described tube bag are fixed together.
Fermentation packaging bag provided by the invention, wherein said breather valve comprises valve body and lamina membranacea, and described valve body is provided with spiracle and claw, and described lamina membranacea is movably arranged between the described claw.
Fermentation packaging bag provided by the invention, wherein said valve body is provided with the oil sealing road, and described lamina membranacea is fitted on the described valve body by described oil sealing road.
Fermentation packaging bag provided by the invention, wherein said lamina membranacea are that the aperture is the micropore lamina membranacea of 0.2-0.6 μ m.
Fermentation packaging bag provided by the invention, wherein said valve body adopt plastics to make; Lamina membranacea adopts composite fibre or plastics or hectograph to make; The tube bag adopts composite plastic film to make; Valve body is fixed together by heat seal with the tube bag.
The characteristics of animal fermented concentrated feed of the present invention and mixed feed and preparation method thereof and equipment are:
1, comprehensive nutrition
Owing to screening in animal pig body and the lactic acid bacteria that cultivate to enlarge, streptococcus fecalis, saccharomycete, with the fermented bacterium fast growth that aspergillus niger, Trichoderma viride, bacillus mixing match are made, anti-assorted bacterium ability is adapted at existence in the stomach of pig and the enteron aisle by force.By taking from lactic acid bacteria (lactic ferments) saccharomycete (saccharomyces cerevisiae) in the animal body, green this mould (tirchoderma) through pure culture and market sale, aspergillus niger mixing such as (aspergillus miger) reaches every gram mixed bacteria more than 1,000,000,000.Be mixed and made into leavening with growth accelerator then, and pig, chicken, ox concentrate feed or batch mixed with leavening, in plastic packaging bag, seal and ferment, this kind technology is made fermented feed, nutriments such as lactic acid bacteria and saccharomycete and various enzyme, crude protein, amino acid all are improved, and cellulase (cellulase) amylase (amylase) that microbial metabolism produces, protease (protease), this dextranase, pectase, enzyme are lived also very high.Because beneficial bacterium can not be survived having under the antibiotic condition, so fermented feed is an antibiotic-free feed, direct feeding animals effect is fine.
2, with low cost
Adopt equipment provided by the invention, the plastic packaging bag that particularly has breather valve adopts the technology of packing after fermentation earlier, and this method is one of current the most succinct zymotechnique.Because plastic packaging bag has had breather valve, feed is bred in a large number at fermentation initial stage aerobic bacteria, the aerogenesis fierceness, and unnecessary gas is discharged by breather valve, a large amount of exhausts of steam vent diameter 3mm during exhalation.When oxygen depletion, this moment, breather valve stopped exhaust, and anaerobic bacteria begins breeding.When feed ph drops to 4 left and right sides, anaerobic bacteria begins dormancy, at this moment, micropore size on the breather valve is at 0.2 ~ 0.6 micron, constantly the micro amount of oxygen molecule is sent in the packaging bag, keep minimum living necessities and various assorted bacterium is blocked in outside the packaging bag for aerobic bacteria, guaranteed various beneficial bacteriums survivals, the feed matter that remains unchanged for a long period of time.This method is to obtain a large amount of beneficial bacteriums and the cheapest method of plurality of enzymes.
Description of drawings
Fig. 1 is used to prepare the fermentation packaging bag front view of concentrated feed of animal or mixed feed for the present invention;
Fig. 2 is used to prepare the fermentation packaging bag side view of concentrated feed of animal or mixed feed for the present invention;
Fig. 3 is the partial enlarged drawing of Fig. 2;
Fig. 4 is the side view of fermentation packaging bag lamina membranacea of the present invention attracting state in valve body;
Fig. 5 is the side view of fermentation packaging bag lamina membranacea of the present invention open mode in valve body;
Fig. 6 prepares the duplex conveyor front view of animal fermented concentrated feed or mixed feed for the present invention;
Fig. 7 prepares the duplex conveyor side view of animal fermented concentrated feed or mixed feed for the present invention.
The specific embodiment
Describe the present invention by the following examples in detail, but the present invention is not limited thereto.
The preparation method of animal fermented concentrated feed provided by the invention and mixed feed, its step is as follows:
One, bacterial classification is cultivated and the leavening making:
Animal concentrates, cooperates the employed main bacterial strain of fermented feed is Bacillus acidi lactici, streptococcus fecalis, bacillus, saccharomycete, Trichoderma viride, aspergillus niger etc.Bacillus acidi lactici, class streptococcus, saccharomycete are wanted to mix after pure culture earlier to form leavening again, and existing is the example explanation with the lactic acid bacteria:
At first, prepare solid potato agar culture medium by following weight proportion: distilled water 1000g, peeling potato 100g, pectin 8g, xylan 6g, ammonium sulfate 4g, PH3.5-4 lactic acid 2g, stand-by through 121 ℃ of 40 minutes sterilization after coagulations.Appoint that to get a body weight be health pig more than 40 kilograms, prepare the easy Microbiological Lab of cover equipment.After the pig of will taking a sample is butchered, get large intestine, the cut-out sampling of small intestine position, two ends are tight with knot, note other position of intestines being damaged.The intestines that downcut are brought in the desinfection chamber, put into medicinal alcohol, took out in 2 minutes, use aseptic water washing, put into sterile barrel then together with operating personnel's both hands immersion.Open the rope at intestines two, take out thing in the intestines, intestinal mucosa is scraped with sterile razor blade, pour the sterilized water dilution into, stir to take out after 2 minutes and filter 50 milliliters, in the frosted wide-mouth bottle after the sterilization of packing into, adopt quartering progressively to be reduced to 1 milliliter, add the sterilized water dilution, being mixed with concentration is 10
2, 10
3, 10
4, 10
5, 10
6Dilution.Above-mentioned dilution is uniformly coated on respectively on each agar medium, and each is coated with 0.5ml, and through 28-30 ℃ of constant temperature culture 5 days, clump count was no more than single bacterium colony of 10 as the preliminary election bacterial strain.With the same method all bacterial classifications are enlarged cultivation, obtain the leavening bacterial classification after spreading cultivation, its Ph is about 5, and beneficial bacterium content is at every gram more than 1,000,000,000.
The preparation of bacterial classification growth accelerator: the bacterial classification growth accelerator is the growth promoter that aims at the bacterial classification preparation, and the component and the percentage by weight thereof of bacterial classification growth accelerator consist of one of following each scheme:
A. FOS 30%,
Lactic acid 40%,
Growth of microorganism element 20%;
B. FOS 40%,
Lactic acid 30%,
Growth of microorganism element 20%;
C. FOS 50%,
Lactic acid 20%,
Growth of microorganism element 20%;
D. FOS 50%,
Lactic acid 30%,
Growth of microorganism element 10%;
E. FOS 30%,
Lactic acid 50%,
Ammonium hydrogen phosphate 5%,
Growth of microorganism element 15%.
The preparation leavening: get bacterial classification 100 grams after spreading cultivation, bacterial classification growth accelerator 900 grams packing composition feed respectively add up to 1000 grams with leavening.
Two, animal fermented concentrated, mixed feed making:
The component and the percentage by weight thereof of animal fermented concentrated feed consist of one of following each scheme:
A. dregs of beans 30%,
Peanut meal 20%,
Corn protein powder 40%,
Calcium monohydrogen phosphate 7%,
Salt 2.8%,
Trace element 0.1%,
Leavening 0.1%;
B. dregs of beans 40%,
Corn protein powder 40%,
Calcium monohydrogen phosphate 7%,
Salt 2.8%,
Trace element 0.1%,
Leavening 0.1%;
C. dregs of beans 50%,
Peanut meal 20%,
Corn protein powder 20%,
Calcium monohydrogen phosphate 7%,
Salt 2.8%,
Trace element 0.1%,
Leavening 0.1%;
D. dregs of beans 37%,
Corn protein powder 40%,
Salt 2.6%,
Trace element 0.2%,
Leavening 0.2%;
E. dregs of beans 37%,
Peanut meal 20%,
Corn protein powder 30%,
Salt 2.6%,
Trace element 0.2%,
Leavening 0.2%.
The component and the percentage by weight thereof of animal fermented mixed feed consist of one of following each scheme:
A. corn 20%,
Dregs of beans 30%,
Fish meal 5%,
Vegetable oil 5%,
Brown sugar 5%,
Calcium monohydrogen phosphate 2.5%,
Salt 0.3%,
Trace element 0.1%,
Leavening 0.1%;
B. corn 30%,
Dregs of beans 30%,
Whey powder 5%,
Peanut meal 5%,
Fish meal 5%,
Vegetable oil 5%,
Brown sugar 5%,
Calcium monohydrogen phosphate 2.5%,
Salt 0.3%,
Trace element 0.1%,
Leavening 0.1%;
C. corn 30%,
Dregs of beans 30%,
Peanut meal 5%,
Fish meal 5%,
Vegetable oil 3%,
Calcium monohydrogen phosphate 2.3%,
Salt 0.3%,
Trace element 0.2%,
Leavening 0.2%;
D. corn 25%,
Dregs of beans 25%,
Fish meal 5%,
Vegetable oil 5%,
Brown sugar 5%,
Calcium monohydrogen phosphate 2.3%,
Salt 0.3%,
Trace element 0.2%,
Leavening 0.2%;
E. corn 30%,
Dregs of beans 20%,
Fish meal 5%,
Vegetable oil 5%,
Brown sugar 5%,
Calcium monohydrogen phosphate 2.3%,
Salt 0.3%,
Trace element 0.2%,
Leavening 0.2%.
Get leavening 1kg, drop in the 25kg temperature milk and stir, quiet putting carried out bacterial classification and brought back to life expansion and grow standby after 15 minutes.After 15 minutes, this strain liquid put in the 200-300kg warm water stir, mix in the middle of putting into 1 ton of concentrate feed or 1 ton of batch, heat sealing in the fermentation packaging bag that has breather valve of packing into, fermentation can be used in 6-30 days between 40 °-10 °.Fermenting mixture is being packed into the fermentation packaging bag initial stage, as at summer temp at 25 degree when above, it is more abundant ferment, in a large amount of gases generations are arranged, as not exhaust as early as possible, probably bag is burst.Exit under the effect of breather valve, when setting exhaust, aperture 3mm is enough to a large amount of exhausts, to guarantee the safety of fermentation packaging bag.Guaranteed the aerobic bacteria eubolism again.When oxygen had consumed, breather valve stopped exhaust, supplied with trace oxygen by breather valve (mechanical hole or liquid hole, aperture 0.2-0.6 micron) in fermentation packaging bag.To satisfy the minimum existence requirement of aerobic bacteria.And assorted bacterium is blocked in outside the polybag, to guarantee that fermented feed is not by living contaminants.Mixture can be finished sweat automatically in the fermentation packaging bag in 6-30 days, and this fermentation packaging bag promptly is that round is again a packing container.
Common have a large amount of reports and use case in fermented bacterium use in the past, but do not hear the bacterial strain of taking from the animal pig body, through the report and the use case of pure culture expansion.So it is common fermented product result of use in the process of raising pigs is not ideal, difficult in popularization.Animal fermented concentrated feed provided by the invention and mixed feed, owing to screening in animal pig body and the lactic acid bacteria that cultivate to enlarge, streptococcus fecalis, saccharomycete, with the fermented bacterium fast growth that aspergillus niger, Trichoderma viride, bacillus mixing match are made, anti-assorted bacterium ability is adapted at existence in the stomach of pig and the enteron aisle by force.
By taking from lactic acid bacteria (lactic ferments) saccharomycete (saccharomyces cerevisiae) in the animal body, green this mould (tirchoderma) through pure culture and market sale, aspergillus niger mixing such as (aspergillus miger) reaches every gram mixed bacteria more than 1,000,000,000.Be mixed and made into leavening with growth accelerator then, and pig, chicken, ox concentrate feed or batch mixed with leavening, in plastic packaging bag, seal and ferment, this kind technology is made fermented feed, nutriments such as lactic acid bacteria and saccharomycete and various enzyme, crude protein, amino acid all are improved, and cellulase (cellulase) amylase (amylase) that microbial metabolism produces, protease (protease), this dextranase, pectase, enzyme are lived also very high.Because beneficial bacterium can not be survived having under the antibiotic condition, so fermented feed is an antibiotic-free feed, direct feeding animals effect is fine.
Step by above-mentioned preparation method prepares animal fermented concentrated feed and mixed feed, and it is fine to be applied in its feeding effect.Test is carried out in Beijing Cui Cun grand plant of shaking, and the time is on November 14,12 days to 2005 September in 2005.
Test pig is selected 100 for use, is divided into 2 groups, 50 of control groups, 50 of test group.
See table:
The comparison of fermented feed feed pigs result sees table:
Illustrate: the premix of control group is Chinese famous brand name, wherein is added with antibiotic, and does not have antibiotic in the test group feed.
Embodiment 3
Referring to Fig. 6-Fig. 7, a kind of duplex conveyor for preparing concentrated feed of animal or mixed feed, comprise top box 10 and be arranged in first of top box 10 and cut with scissors cage 11 and drive motors 13 thereof, top box 10 belows are provided with the hopper 14 that is fixed together with it, the bottom of hopper is provided with second hinge cage 12 and the drive motors 15 thereof, and the below of hopper 14 is provided with discharging opening 16.The hopper 14 and the second hinge cage 12 are provided with 2 covers, constitute duplex conveyor.The bottom of top box 10 is equipped with dodge gate 17 and drive motors 18 thereof.The hopper 14 and the second hinge cage 12 are provided with 2 covers; Top box 10, hopper 14, the first hinge cage 11 and the second hinge cage 12 adopt stainless steel to make.
Embodiment 4
Referring to Fig. 1-Fig. 3, a kind of fermentation packaging bag that is used to prepare concentrated feed of animal or mixed feed comprises tube bag 2, and tube bag 2 one ends are provided with heat sealing 1, and its bag is provided with at least one through hole, is coated with breather valve 4 on the through hole.Referring to Fig. 4-Fig. 5, breather valve 4 comprises valve body 41 and lamina membranacea 47, and valve body 41 is provided with 45,4 claws 43 of spiracle and oil sealing road 46, and lamina membranacea 47 is fitted on the valve body 41 by oil sealing road 46, and lamina membranacea 47 is movably arranged between the claw 43.Lamina membranacea 47 is the micropore lamina membranacea of 0.2-0.6 μ m for the aperture.Valve body 41 adopts plastics to make; Lamina membranacea 47 adopts composite fibre to make.In other embodiments, also can adopt plastics or hectograph to make.Tube bag 2 adopts composite plastic film to make.Valve body 41 and tube bag 2 are fixed together by heat seal.
Adopt equipment provided by the invention to prepare concentrated feed of animal or animal mixed feed, particularly plastic packaging bag, can fully guarantee the quality of product.Because plastic packaging bag has had breather valve, feed is bred in a large number at fermentation initial stage aerobic bacteria, the aerogenesis fierceness, and unnecessary gas is discharged by breather valve, a large amount of exhausts of steam vent diameter 3mm during exhalation.When oxygen depletion, this moment, breather valve stopped exhaust, and anaerobic bacteria begins breeding.When feed ph drops to 4 left and right sides, anaerobic bacteria begins dormancy, at this moment, micropore size on the breather valve lamina membranacea is at the 0.2-0.6 micron, constantly the micro amount of oxygen molecule is sent in the strap, keep minimum living necessities and various assorted bacterium is blocked in outside the packaging bag for aerobic bacteria, guaranteed various beneficial bacteriums survivals, the feed matter that remains unchanged for a long period of time.This method is to obtain a large amount of beneficial bacteriums and the cheapest method of plurality of enzymes.
Claims (2)
1. the preparation method of an animal fermented concentrated feed is characterized in that, the step of this method is as follows:
One, preparation bacterial strain:
(1) healthy live hog is butchered, got thing and mucous membrane in large intestine, small intestine, the stomach under the aseptic condition, add the sterilized water dilute filtration and take out 50 milliliters;
(2) adopt quartering progressively to taper to 1 milliliter, add the sterilized water dilution, being mixed with concentration is 10
2, 10
3, 10
4, 10
5, 10
6Dilution;
(3) described dilution is uniformly coated on respectively on a plurality of solid potato agar culture mediums, each is coated with 0.5ml;
(4) through 28-30 ℃ of constant temperature culture 5 days, clump count was no more than single bacterium colony of 10 as the preliminary election bacterial strain;
Two, preparation leavening:
(1) the purebred nutrient solution that spreads cultivation of preparation: the purebred nutrient solution that spreads cultivation is by containing sucrose 40g, glucose 40g, bean cake powder 40g, corn flour 40g, potassium dihydrogen phosphate 3g preparation among every 1000g, nutrient solution was 120 ℃ of high-temperature sterilizations 40 minutes, be cooled to after 35 ℃, by 0.1% weight ratio inoculating strain;
(2) purebred spreading cultivation: lactic acid bacteria 34-36 ℃ constant temperature culture 8 days, viable lactic acid bacteria quantity is 1 * 10
8Individual/more than the gram; Saccharomycete was 34-36 ℃ of constant temperature culture 6 days, and the saccharomycete number of viable is 10
6Individual/more than the gram; The lactic acid bacteria, streptococcus fecalis, the saccharomycete that use to cultivate enlarge and obtain the leavening bacterial classification after aspergillus niger, Trichoderma viride, bacillus mix, and its Ph is about 5;
(3) preparation bacterial classification growth accelerator: the component and the percentage by weight thereof of bacterial classification growth accelerator consist of:
FOS 30-50%,
Lactic acid 20-40%,
Ammonium hydrogen phosphate 5-10%,
The plain 5-20% of growth of microorganism;
(4) preparation leavening: get bacterial classification 100 gram after spreading cultivation, get bacterial classification growth accelerator 900 grams and add up to 1000 grams to be packaged into leavening;
Three. the preparation animal fermented concentrated feed:
1) component of animal fermented concentrated feed and percentage by weight thereof consist of:
Dregs of beans 30-50%,
Peanut meal 5-20%,
Corn protein powder 20-40%,
Calcium monohydrogen phosphate 5-10%,
Salt 2-3%,
Trace element 0.1-0.2%,
Leavening 0.1-0.2%;
2) step of preparation animal fermented concentrated feed:
(1) leavening is put into 30 ℃ of milk and stirred, placed then 15 minutes, carry out pouring in the warm water as strain liquid after bacterial classification resurrection expansion is grown;
(2) mix heat sealing in the fermentation packaging bag that has breather valve of packing in the middle of above-mentioned strain liquid being put into the concentrate feed of the component that meets in the step 1) and percentage by weight;
(3) in room temperature 10-40 ℃ scope after 15-30 days the fermentation, animal fermented concentrated feed can use.
2. the preparation method of an animal fermented mixed feed is characterized in that, the step of this method is as follows:
One, preparation bacterial strain:
(1) healthy live hog is butchered, got thing and mucous membrane in large intestine, small intestine, the stomach under the aseptic condition, add the sterilized water dilute filtration and take out 50 milliliters;
(2) adopt quartering progressively to taper to 1 milliliter, add the sterilized water dilution, being mixed with concentration is 10
2, 10
3, 10
4, 10
5, 10
6Dilution;
(3) described dilution is uniformly coated on respectively on a plurality of solid potato agar culture mediums, each is coated with 0.5ml;
(4) through 28-30 ℃ of constant temperature culture 5 days, clump count was no more than single bacterium colony of 10 as the preliminary election bacterial strain;
Two, preparation leavening:
(1) the purebred nutrient solution that spreads cultivation of preparation: the purebred nutrient solution that spreads cultivation is by containing sucrose 40g, glucose 40g, bean cake powder 40g, corn flour 40g, potassium dihydrogen phosphate 3g preparation among every 1000g, nutrient solution was 120 ℃ of high-temperature sterilizations 40 minutes, be cooled to after 35 ℃, by 0.1% weight ratio inoculating strain;
(2) purebred spreading cultivation: lactic acid bacteria 34-36 ℃ constant temperature culture 8 days, viable lactic acid bacteria quantity is 1 * 10
8Individual/more than the gram; Saccharomycete was 34-36 ℃ of constant temperature culture 6 days, and the saccharomycete number of viable is 10
6Individual/more than the gram; The lactic acid bacteria, streptococcus fecalis, the saccharomycete that use to cultivate enlarge and obtain the leavening bacterial classification after aspergillus niger, Trichoderma viride, bacillus mix, and its Ph is about 5;
(3) preparation bacterial classification growth accelerator: the component and the percentage by weight thereof of bacterial classification growth accelerator consist of:
FOS 30-50%,
Lactic acid 20-40%,
Ammonium hydrogen phosphate 5-10%,
The plain 5-20% of growth of microorganism;
(4) preparation leavening: get bacterial classification 100 gram after spreading cultivation, get bacterial classification growth accelerator 900 grams and add up to 1000 grams to be packaged into leavening;
Three. prepare animal fermented mixed feed:
1) component of animal fermented mixed feed and percentage by weight thereof consist of:
Corn 20-30%,
Dregs of beans 20-30%,
Whey powder 5-10%,
Peanut meal 5-10%,
Fish meal 2-5%,
Vegetable oil 2-5%,
Brown sugar 2-5%,
Corn protein powder 5-15%,
Calcium monohydrogen phosphate 2-3%,
Salt 0.2-0.5%,
Trace element 0.1-0.2%,
Leavening 0.1-0.2%;
2) step of the animal fermented mixed feed of preparation:
(1) leavening is put into 30 ℃ of milk and stirred, placed then 15 minutes, carry out pouring in the warm water as strain liquid after bacterial classification resurrection expansion is grown;
(2) mix heat sealing in the fermentation packaging bag that has breather valve of packing in the middle of above-mentioned strain liquid being put into the concentrate feed of the component that meets in the step 1) and percentage by weight;
(3) in room temperature 10-40 ℃ scope after 7-30 days the fermentation, animal fermented mixed feed can use.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100023899A CN100546491C (en) | 2006-01-27 | 2006-01-27 | Animal fermented concentrated feed and mixed feed and preparation method thereof and equipment |
| PCT/CN2006/001134 WO2007085142A1 (en) | 2006-01-27 | 2006-05-29 | Animal fermented concentrated feed and compound feed, manufacturing methods, and apparatus thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100023899A CN100546491C (en) | 2006-01-27 | 2006-01-27 | Animal fermented concentrated feed and mixed feed and preparation method thereof and equipment |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009101582390A Division CN101611919B (en) | 2006-01-27 | 2006-01-27 | Fermentation packaging bag |
| CNA2009100007993A Division CN101513219A (en) | 2006-01-27 | 2006-01-27 | Duplex conveyor for preparing fermented concentrated feed or compound feed for animals |
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| CN1857099A CN1857099A (en) | 2006-11-08 |
| CN100546491C true CN100546491C (en) | 2009-10-07 |
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| WO (1) | WO2007085142A1 (en) |
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| CN102060148A (en) * | 2010-12-07 | 2011-05-18 | 宜章县上田农村经济开发有限公司 | Method for packing microbial fermented feed |
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| CN2224658Y (en) * | 1995-04-28 | 1996-04-17 | 江西省食品机械研究所 | Cake crasher with cutting tooth and hammer |
| SE511025C2 (en) * | 1997-10-03 | 1999-07-26 | Probi Ab | Horse feed product comprising Lactobacillus plantarum JI: 1 and Lactobacillus plantarum JI: 1 and use thereof |
| CN2483348Y (en) * | 2001-04-06 | 2002-03-27 | 郑道付 | Automatic air-pumping out type vertical vacuum package bag |
| KR20030000243A (en) * | 2001-06-22 | 2003-01-06 | 이 석 민 | Method for manufacturing animal food and plastic bag type air-tight fermentation container suitable for the same |
| CN1283170C (en) * | 2002-11-12 | 2006-11-08 | 上海创博生态工程有限公司 | Enzyme-enriched prebiotics and its preparation |
| CN1511945A (en) * | 2002-12-30 | 2004-07-14 | 中国农业大学 | Lactic bacillus strain and its separating and breeding method and use |
| CN1593771A (en) * | 2004-07-06 | 2005-03-16 | 王一留 | Super energy-saving integrated fine grain pulverizer |
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