CN100535643C - 一种双色荧光定量共扩增聚合酶链反应检测试剂盒 - Google Patents

一种双色荧光定量共扩增聚合酶链反应检测试剂盒 Download PDF

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Publication number
CN100535643C
CN100535643C CNB2007100143908A CN200710014390A CN100535643C CN 100535643 C CN100535643 C CN 100535643C CN B2007100143908 A CNB2007100143908 A CN B2007100143908A CN 200710014390 A CN200710014390 A CN 200710014390A CN 100535643 C CN100535643 C CN 100535643C
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China
Prior art keywords
gapdh
primer
dscr1
probe
pcr
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Chinese (zh)
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CN101105454A (zh
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夏庆杰
杨林
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SHANDONG YADA PHARMACEUTICAL CO Ltd
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SHANDONG YADA PHARMACEUTICAL CO Ltd
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Priority to CNB2007100143908A priority Critical patent/CN100535643C/zh
Publication of CN101105454A publication Critical patent/CN101105454A/zh
Priority to PCT/CN2008/000871 priority patent/WO2008134933A1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNB2007100143908A 2007-04-29 2007-04-29 一种双色荧光定量共扩增聚合酶链反应检测试剂盒 Active CN100535643C (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CNB2007100143908A CN100535643C (zh) 2007-04-29 2007-04-29 一种双色荧光定量共扩增聚合酶链反应检测试剂盒
PCT/CN2008/000871 WO2008134933A1 (fr) 2007-04-29 2008-04-29 Trousse de fluorescence à deux couleurs utiles pour détecter la réaction en chaîne de la polymérase de coamplification

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNB2007100143908A CN100535643C (zh) 2007-04-29 2007-04-29 一种双色荧光定量共扩增聚合酶链反应检测试剂盒

Publications (2)

Publication Number Publication Date
CN101105454A CN101105454A (zh) 2008-01-16
CN100535643C true CN100535643C (zh) 2009-09-02

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CNB2007100143908A Active CN100535643C (zh) 2007-04-29 2007-04-29 一种双色荧光定量共扩增聚合酶链反应检测试剂盒

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CN (1) CN100535643C (fr)
WO (1) WO2008134933A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104087672A (zh) * 2014-07-14 2014-10-08 钦州市妇幼保健院 一种多重实时荧光定量pcr技术快速检测人21号染色体数目的试剂盒
CN106755443B (zh) * 2016-12-30 2018-11-30 人和未来生物科技（长沙）有限公司 一种从多种混合dna中超灵敏检测痕量dna的方法和试剂
CN109628559B (zh) * 2019-01-29 2023-05-02 阅尔基因技术（苏州）有限公司 一种检测y染色体拷贝数变异的方法和试剂盒

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
双色实时荧光定量PCR及荧光原位杂交检测唐式综合征. 王玲.中国优秀博硕士学位论文全文数据库(硕士) 医药卫生科技辑，第01期. 2006
双色实时荧光定量PCR及荧光原位杂交检测唐式综合征. 王玲.中国优秀博硕士学位论文全文数据库(硕士) 医药卫生科技辑，第01期. 2006 *
实时荧光定量聚合酶链反应技术在产前诊断唐式综合征总的应用. 郑明明等.中华妇产科杂志，第39卷第10期. 2004
实时荧光定量聚合酶链反应技术在产前诊断唐式综合征总的应用. 郑明明等.中华妇产科杂志，第39卷第10期. 2004 *
应用PCR-STR分型技术快速产前基因诊断Down综合征. 朱金玲等.中国优生与遗传杂志，第12卷第6期. 2004
应用PCR-STR分型技术快速产前基因诊断Down综合征. 朱金玲等.中国优生与遗传杂志，第12卷第6期. 2004 *

Also Published As

Publication number Publication date
CN101105454A (zh) 2008-01-16
WO2008134933A1 (fr) 2008-11-13

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