CN100523204C - Anaerobic biological acidifying transform process for spartina alterniflora - Google Patents
Anaerobic biological acidifying transform process for spartina alterniflora Download PDFInfo
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- CN100523204C CN100523204C CNB2006101161434A CN200610116143A CN100523204C CN 100523204 C CN100523204 C CN 100523204C CN B2006101161434 A CNB2006101161434 A CN B2006101161434A CN 200610116143 A CN200610116143 A CN 200610116143A CN 100523204 C CN100523204 C CN 100523204C
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- alterniflora loisel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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Abstract
The present invention belongs to the field of resource and environment protecting technology, and is especially anaerobic biological acidifying transform process for Spartina alterniflora. The process includes the following steps: cutting and kneading Spartina alterniflora material, throwing into acid-generating fermentation apparatus, adding certain amount of N element to regulate C/N ratio and inoculating to start reaction so as to produce microbial fermentation at anaerobic condition and generate acid, and extracting acid solution periodically for post treatment. The acid solution may have several uses, such as being used as feed additive, for producing methane through fermentation, for producing hydrogen treat fermentation, etc. The present invention utilizes Spartina alterniflora resource effectively and changes harmful matter into useful matter.
Description
Technical field
The invention belongs to the resource and environment technical field, be specifically related to the method that a kind of spartina alterniflora loisel's anaerobic biological acidifying transforms.
Background technology
Spartina alterniflora loisel (Spartina aterniflora) originates in the U.S., is a kind of plant of sabkha for many years that is grown in the tideland.At the initial stage in the 1980's, the spartina alterniflora loisel is introduced into China and is used to protect beach shore protection, created land with silt, and once to protection sea wall safety, many-sides such as the improvement of promotion economy development of coastal area, ecotope and mitigation had been made major contribution to spartina alterniflora loisel's introduction.But through 20 years of development, the spartina alterniflora loisel forms 80-100 in China's Coastal Areas and gets group for ten thousand mu, and the advantage group so that this Alien invasive species forms brings a series of negative impact to the coastland.As alien species with extremely strong fecundity, it is prosperous that the spartina alterniflora loisel has growth, breeding is fast, plant Gao Ermi with native country species dog-eat-dog, has changed the view on mud matter light beach and the habitat of native country species, for example the spartina alterniflora loisel occupies the place that intertidal shellfish is cultured, cause the shellfish carelessly can't be free movable in the beach at intensive rice, healthy growth, even can death by suffocation; Some shellfishes have to migrate to rice carelessly outside the beach; Some harbor districts also because spartina alterniflora loisel's intrusion alluvial is accelerated, influence ship and come in and go out.Summer and autumn the spartina alterniflora loisel fallen leaves along with tidewater drifts about everywhere, enter the algal culture ground in beach or shallow sea, will be to laver, production of marine algas such as sea-tangle and the certain influence of the quality of product generation.
The spartina alterniflora loisel is grown in the C4 plant in the high mineral environment in tideland, in the long-term process that adapts to, has formed high productivity and high resistance to cold and diseases.According at northern Suzhou and two places, Shanghai years of researches, spartina alterniflora loisel's unit surface over-ground part crop can reach 2.5-3kg/m in the northern Suzhou
2, i.e. 1600-2000kg/ mu; The area, Shanghai can reach 3-5kg/m
2, i.e. 2000-3333kg/ mu.Under the condition of good field management, three great tradition food crop and per unit area yield thereof (containing stalk output) are respectively: wheat 500-600kg/ mu, paddy rice 600-1000kg/ mu, corn 800-1200kg/ mu at present.Drop under the condition of managing without any artificial, the spartina alterniflora loisel almost reaches 2-3 times of the good field management output of traditional crop, if be aided with fertilising and behavior adjustment management, the spartina alterniflora loisel can obtain higher unit output.The spartina alterniflora loisel is productivity so efficiently, as changing into product and raw material, can largely benefit coastal resident.
Current, spartina alterniflora loisel's utilization research research just begins, because spartina alterniflora loisel's saltiness is too high, does not also have the sophisticated approach that utilizes to be in the news at present.Moreover, it is all very limited to be similar to the present progress of spartina alterniflora loisel's the organic utilization of herbaceous plant stalk, and some bibliographical informations utilize herbaceous plant to produce ethanol by bio-transformation, also has report to produce biogas and hydrogen etc., but reaction conditions is all comparatively harsh, complex management.According to the research of microbial fermentation as can be known, be very easy to utilize various organic matters to carry out fermentation and acid, and the organic acid that produces can multiple industry use in the anaerobic condition microorganism.
Summary of the invention
The object of the present invention is to provide a kind of can be under open or semi-open condition, manage the method that spartina alterniflora loisel's anaerobic biological acidifying very easily transforms.
The method that spartina alterniflora loisel's anaerobic biological acidifying that the present invention proposes transforms, its concrete steps are as follows:
(1) raw material spartina alterniflora loisel's pulverizing pre-treatment;
(2) interpolation contains the N elemental substance in the pre-treatment spartina alterniflora loisel of step (1) gained, mixes, and the carbon-nitrogen ratio of control compound is 20:1--30:1;
(3) step (2) gained compound is put in the bio-acidification fermentation unit, added inoculum and inoculate, the add-on of inoculum is the 3%-5% of spartina alterniflora loisel's raw material dry weight;
(4) add water in the fermentation raw material of gained in step (3) and ferment, the add-on of water is that the water surface is higher than fermentation raw material 20cm at least, and leavening temperature is controlled at 20-40 ℃;
(5) after the fermentation of the bio-acidification in the step (4) is carried out 2-5 days, fermented liquid pH value in the fermentation unit drops to 5-6, VFA (VFA) is 30-50mol/l, beginning is periodically extracted 10%-100% and is contained acid-fermentation liquid and be used for later use from fermentation unit, add simultaneously and the make up water of extracting the acid solution equal volume, carry out continuing fermentation and produce acid;
(6) the fermented liquid pH value in fermentation unit drops to 5-6, weight step (5);
(7) whole fermentation period is 15-30 days, and the VFA of the fermented liquid in the bio-acidification reactor drops to below the 10mol/l, stops acidogenic fermentation.
Among the present invention, the described N of containing elemental substance is an one to multiple kind in urea, bicarbonate of ammonia or the feces of livestock and poultry etc.
Among the present invention, described inoculum be in natural pond slag, natural pond liquid or the anaerobically digested sludge etc. any.
Among the present invention, the pre-treatment of described spartina alterniflora loisel's raw material pulverizing can be taked following ordinary method: with harvested spartina alterniflora loisel's over-ground part, be cut into the segment of 2～3cm by artificial or mechanical means; Perhaps it is carried out pre-treatment etc. by stalk rubbing pulverizer.
Among the present invention, make up water described in the step (5) be in seawater, river, lake water or the tap water etc. one or more.
Among the present invention, extract the fermentation acid solution described in the step (5) and can use pump to extract out or flow automatically fermentation unit by fermentation acid solution self gravitation.
Among the present invention, the bio-acidification reaction unit will guarantee that the edge is higher than local most torrential rain depth of immersion, and fermentation reaction device top 1.5-3 rice height will be built rainproof overcover.
Among the present invention, in the biological acidification conversion reaction, the bio-acidification reaction unit does not carry out definitely airtight, but for oxygen exchange that reduces liquid level and the smell that reduces organic acid volatilization initiation, plastics film can tile on the liquid level in reactor.
Among the present invention, the later use of extracting out in the bio-acidification reaction unit that contains acid-fermentation liquid can have multiple use, as can be used for fodder additives, fermentation methane production, fermentation and hydrogen production etc.
Advantage of the present invention: utilize the plant of this high productivity of spartina alterniflora loisel to ferment, be rich in organic acid and mineral substance in the fermented liquid after the fermentation.Organic acid can improve the acidity of feed. reduce the surge capability of feed, have sterilization, antibacterial, the effect that prevents feed mold simultaneously, can be added in feed or the drinking-water and play a role, be widely used in the feed of pig, chicken, also have increasing research to use in the feed of ox, fish.Along with antibiotic forbidding, organic acid substitutes microbiotic.Thereby produce the danger that acts synergistically resistance with some microbiotic and be reduced to the attention that MIN effectiveness obtains people.Add organic acid in the feed and can promote trace elements absorbed utilizations such as iron, copper, alleviate its pollution environment.Organic acid has the effect that some microbiotic do not have, and comprises pH value that can reduce chyme and the secretion that strengthens pancreatic juice.Organic acid solution also can be used for fermentation and hydrogen production, fermentation methane production, the useful medicine of extraction and trace element etc.
Description of drawings
Fig. 1 is the changing conditions that embodiment 1 acidifying transforms acid solution VFA and COD.
Fig. 2 is the changing conditions that embodiment 2 acidifyings transform acid solution VFA and COD.
The real formula of concrete enforcement
Further specify the present invention below by embodiment.
Embodiment 1
(1) builds 1 cube of fluid-tight hole or pond body, set up rainproof coverture above, as the bio-acidification fermentation unit;
(2) pre-treatment of raw material:, be cut into the segment of 2～3cm by mechanical means with harvested spartina alterniflora loisel's over-ground part;
(3) being added in the bio-acidification fermentation unit through pretreated raw material 60kg spartina alterniflora loisel (dry weight) in the step (2), add 1.2kg urea, mix, the C:N that regulates compound is than being 25:1;
(4) inoculation: answer the requirement of later use, in the anaerobically digested sludge 10kg input bio-acidification fermentation unit with inoculation water content 80%, inoculum size is 3.33%;
(5) add water in the bio-acidification fermentation unit and ferment, add-on is that the water surface is higher than fermentation raw material 20cm, and leavening temperature is 40 ℃;
(6) after the bio-acidification fermentation was carried out 3 days, the fermented liquid pH value in the fermentation unit dropped to 5.64, began to extract 0.3 cube of fermented liquid from fermentation unit, added 0.3 cubical tap water to the bio-acidification fermentation unit simultaneously and supplied the water yield, carried out continuing fermentation and produced acid;
(7) after pH drops to below 6, extract 0.3 cube of fermented liquid again out from fermentation unit, add simultaneously with the tap water that extracts the acid solution equal volume and supply the water yield, so the acid solution displacement is carried out in circulation;
(8) whole fermentation was carried out 23 days, and the VFA of the fermented liquid in the bio-acidification reactor drops to below 8.6, and COD drops to below the 1546mg/l, stops acidogenic fermentation.
In the whole process, the VFA of Extract and COD monitoring result are seen Fig. 1.
Embodiment 2
(1) builds 1 cube of fluid-tight hole or pond body, set up rainproof coverture above, as the bio-acidification fermentation unit
(2) pre-treatment of raw material:, be cut into the segment of 2～3cm by mechanical means with harvested spartina alterniflora loisel's over-ground part.
(3) being added in the bio-acidification fermentation unit through pretreated raw material 40kg (dry weight) in the step (2), add 0.6kg bicarbonate of ammonia, mix, the C:N that regulates compound is than being 30:1.
(3) inoculation: answer the requirement of later use, inoculation drops into the natural pond slag 8kg of water content 80% in the bio-acidification fermentation unit;
(4) add water in the bio-acidification fermentation unit and ferment, add-on is that the water surface is higher than fermentation raw material 20cm, and leavening temperature is 25 ℃;
(5) after the bio-acidification fermentation was carried out 3 days, the fermented liquid pH value in the fermentation unit dropped to 5.35, began to extract 0.4 cube of fermented liquid from fermentation unit, added 0.3 cubical seawater to the bio-acidification fermentation unit simultaneously and supplied the water water yield, carried out continuing fermentation and produced acid;
(6) after the fermented liquid pH value in the fermentation unit drops to below 6, from fermentation unit, extract 0.4 cube of fermented liquid once more out, add simultaneously with the seawater that extracts the acid solution equal volume and supply the water yield; So circulation;
(7) whole fermentation was carried out 20 days, and the VFA of the fermented liquid in the bio-acidification reactor drops to 10, and COD drops to below the 1900mg/l, stops acidogenic fermentation.
In the whole process, the VFA of Extract and COD monitoring result are seen Fig. 2.
Claims (6)
1, a kind of method of spartina alterniflora loisel's anaerobic biological acidifying conversion is characterized in that concrete steps are as follows:
(1) raw material spartina alterniflora loisel's pulverizing pre-treatment;
(2) interpolation contains the N elemental substance in the pre-treatment spartina alterniflora loisel of step (1) gained, mixes, and the carbon-nitrogen ratio of control compound is 20:1--30:1;
(3) step (2) gained compound is put in the bio-acidification fermentation unit, added inoculum and inoculate, mix, the add-on of inoculum is the 3%-5% of spartina alterniflora loisel's raw material dry weight;
(4) add water in the fermentation raw material of gained in step (3) and ferment, the add-on of water is that the water surface is higher than fermentation raw material 20cm at least, the plastics film that on the liquid level of fermentation unit, tiles simultaneously, and leavening temperature is 20-40 ℃;
(5) after the fermentation of the bio-acidification in the step (4) is carried out 2-5 days, fermented liquid pH value in the fermentation unit drops to 5-6, voltaile fatty acid is to 30-50mol/l, beginning is periodically extracted 10%-100% and is contained acid-fermentation liquid and be used for later use from fermentation unit, add simultaneously with extraction and contain the make up water of acid-fermentation liquid phase, carry out continuing fermentation and produce acid with volume;
(6) drop to below 6 repeating step (5) when the fermented liquid pH value in the fermentation unit;
(7) whole fermentation is 15-30 days, and the voltaile fatty acid of the fermented liquid in the bio-acidification reactor drops to below the 10mol/l, stops acidogenic fermentation.
2, spartina alterniflora loisel anaerobe according to claim 1 produces the method that acid transforms, and it is characterized in that the described N of containing elemental substance is a kind of in urea, bicarbonate of ammonia or the feces of livestock and poultry.
3, spartina alterniflora loisel anaerobe according to claim 1 produces the method that acid transforms, it is characterized in that described inoculum be in natural pond slag, natural pond liquid or the anaerobically digested sludge any.
4, spartina alterniflora loisel anaerobe according to claim 1 produces the method that acid transforms, and it is characterized in that the pre-treatment of described spartina alterniflora loisel's raw material pulverizing takes harvested spartina alterniflora loisel's over-ground part is cut into the segment of 2～3cm by artificial or mechanical means; Perhaps it is carried out pre-treatment by stalk rubbing pulverizer.
5, the method for spartina alterniflora loisel's anaerobic biological acidifying conversion according to claim 1 is characterized in that make up water described in the step (5) is one or more of seawater, river, lake water or tap water.
6, the method that transforms of spartina alterniflora loisel's anaerobic biological acidifying according to claim 1, the acid-fermentation liquid that contains that it is characterized in that extracting described in the step (5) uses pump to extract out or flow automatically and fermentation unit by containing acid-fermentation liquid self gravitation.
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| CNB2006101161434A CN100523204C (en) | 2006-09-18 | 2006-09-18 | Anaerobic biological acidifying transform process for spartina alterniflora |
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| CNB2006101161434A CN100523204C (en) | 2006-09-18 | 2006-09-18 | Anaerobic biological acidifying transform process for spartina alterniflora |
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| CN100523204C true CN100523204C (en) | 2009-08-05 |
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| CN104512989A (en) * | 2013-09-27 | 2015-04-15 | 南京宏博环保实业有限公司 | Process capable of increasing gas yield of biogas |
| CN107751568A (en) * | 2017-10-31 | 2018-03-06 | 南京工业大学大丰海洋产业研究院 | A kind of method that Spartina alterniflora's biofermentation prepares Livestock feed |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6087547A (en) * | 1999-05-25 | 2000-07-11 | University Of South Carolina | Method for decomposing toxic organic pollutants |
| CN1742545A (en) * | 2005-09-29 | 2006-03-08 | 南京大学 | Method for controlling foreign spartina alterniflora loisel by biological substitution |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6087547A (en) * | 1999-05-25 | 2000-07-11 | University Of South Carolina | Method for decomposing toxic organic pollutants |
| CN1742545A (en) * | 2005-09-29 | 2006-03-08 | 南京大学 | Method for controlling foreign spartina alterniflora loisel by biological substitution |
Non-Patent Citations (1)
| Title |
|---|
| 综合开发利用互花米草. 董国.农牧产品开发，第5期. 1996 * |
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Granted publication date: 20090805 Termination date: 20170918 |