CN100520341C - 液滴操作装置 - Google Patents

液滴操作装置 Download PDF

Info

Publication number
CN100520341C
CN100520341C CNB038201623A CN03820162A CN100520341C CN 100520341 C CN100520341 C CN 100520341C CN B038201623 A CNB038201623 A CN B038201623A CN 03820162 A CN03820162 A CN 03820162A CN 100520341 C CN100520341 C CN 100520341C
Authority
CN
China
Prior art keywords
drop
substrate
droplets
operating means
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB038201623A
Other languages
English (en)
Other versions
CN1678896A (zh
Inventor
安田贤二
一木隆范
冈野和宣
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yasuda Kenji
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CN1678896A publication Critical patent/CN1678896A/zh
Application granted granted Critical
Publication of CN100520341C publication Critical patent/CN100520341C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3031—Micromixers using electro-hydrodynamic [EHD] or electro-kinetic [EKI] phenomena to mix or move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N2035/0097—Control arrangements for automatic analysers monitoring reactions as a function of time
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Fluid Mechanics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

液滴操作装置，其包括具有光透过性，实施了防水处理的基板；向该基板上供给液滴的装置；搬运防水基板上的液滴的装置；具有测定液滴状态的装置的系统，例如，包括表面实施了防水处理并具有光透过性的基板11，将试剂或试料的液滴51导入基板11上的溶液送入口21、22、23，光学测定单元61、62、63，外加电场单元71、72、73等，可以显微镜观察液滴的内部，使液滴静止或使其沿任意的方向移动，使液滴与其他液滴反应，对其反应结果测定、分离。

Description

液滴操作装置
技术领域
本发明申请涉及用于操作液滴的新型装置。
背景技术
将培养液中的特定细胞分离、回收在生物学、医学分析上是重要的技术。通常，当因细胞的比重不同而分离细胞时，可以采用速度沉降法进行分离。但是，当如辨别未过敏反应细胞和过敏反应细胞这样，细胞几乎无差异时，必须以用荧光抗体染色的信息或目视的信息为基础将细胞一个个地分离。对于该技术，例如包括细胞分类器(cellsorter)。细胞分类器是通过将荧光染色处理后的细胞以1细胞单位分离滴入具有电荷的液滴中，以该液滴中的细胞有无荧光、光散射量的大小为基础，在液滴下落的过程中在下落方向的法平面方向上以任意方向外加高电场，从而控制液滴的下落方向，分离到置于下部的多个容器中进行回收的技术。对于该技术，Kamarck，M.E.在MethodsEnzymol.第151卷第150页～165页(1987年)有详细的报告。
发明内容
上述以往使用细胞分类器的细胞分离技术使目前为止不可能的以液滴单位的光学信息为基础的观测成为可能，但对自由下落的液滴的内部状态进行光学上显微观察仍是困难的。特别是没有直接确认是否液滴中实际上只含有一个细胞的方法，而且只能在下落的液滴的法平面方向上作用电场，因此基本上只能调节液体下落的角度。此外，为了使高速下落的液滴在短时间内偏转足够的角度，必须作用2000V以上的高电场。此外，采用以往的细胞分类器计测时，计测只是1次，不能使反应液与分离的液滴混合，进而根据其结果进行分离。
因此，本发明申请的课题在于消除以上所述的以往技术的问题，提供新型的液滴操作装置，该液滴操作装置使对含有细胞或微生物的液滴的内部进行观察成为可能，而且使为此而使上述液滴静止，或使其在任意的方向上移动，对同一液滴进行多次光学计测，使液滴与其他液滴反应并计测其反应结果并进行分离等成为可能。
本发明申请为了解决上述课题，提供如下液滴操作装置，其具有在表面实施了防水处理的绝缘性基板上配置多个电极，向该基板上供给液滴的装置、通过向上述多个电极外加直流或交流电场而将防水基板上的液滴搬运的装置、测定液滴状态的装置。
此外，本发明申请提供如下液滴操作装置，其具有使用了显微镜所成光学像或光传感器等测定液滴的pH、离子浓度、吸光度、荧光等的装置作为测定上述液滴状态的装置，此外，还提供具有使多个液滴混合的装置的液滴操作装置。
附图说明
图1为表示本发明申请的基本构成的模式图。
图2为表示本发明申请的实施例的模式图。
图中的符号表示如下内容。
1：绝缘基板
2、3：电极
4：绝缘层
5：液滴
6：物镜
11：基板
12、13、14、15、16：沟
21、22、23：溶液送入口
31、32、33：溶液排出口
41、42、43、44：电子电路
51：液滴
61、62、63：光学测定单元
71、72、73：外加电场单元
81、82、83：液滴导管
91、92、93：脚
具体实施方式
本发明申请具有如上所述的特征，以下对其实施方式进行说明。
首先，使用图1对本发明申请的液滴操作装置的基本构成进行说明。在该例示中，以覆盖配置于绝缘基板1上的多个电极2、3的形式配置绝缘层4。绝缘层表面实施防水处理，对于此时的防水处理可以适当，例如可以考虑采用硅系树脂或氟系树脂涂布的方法、或在基板表面上形成具有不规则碎片形(フラクタル)结构的凹凸面从而赋予防水性的方法等各种方法。
此外，含有细胞或微生物的液滴5以球状液滴的状态保持在基板上。与细胞分类器相同，当向液滴外加电荷时，通过向电极2、3外加直流电场可以控制液滴的移动方向。此外，在没有外加电荷的液滴的情况下，例如，通过在电极2、3之间外加交流电场，可以使细胞或微生物移动。此外，在对液滴中的细胞进行观察时，通过物镜6可以用位相差显微镜像或荧光显微镜像直接对液滴内的细胞或微生物的状态进行观察。为了光学测定，防水处理的基板可以具有光透过性，对于电极也可以同样为透光性的电极。
以下根据图2所示的模式图对实施例的计测例进行说明。
在该实施例所示的装置中，其具备表面实施了防水处理的基板11，将试剂或试料的液滴导入基板上的溶液插入口21、22、23，搬运液体时粗略控制液体行进方向的沟12、13、14、15、16，将计测、分离后的液滴排出的溶液排出口31、32、33，控制基板上的元件的电子电路41、42、43、44，光学测定液滴的状态的光学测定单元61、62、63，控制液滴51的移动方向的外加电场单元71、72、73，防止液滴逾越的液滴导管81、82、83和基板的脚91、92、93。因此，例如，从溶液插入口21导入的液滴51边沿沟12顺着基板11的缓缓的倾斜滚动，边导入第1光学测定单元，根据测定的液滴的状态，在与沟13的交叉点通过外加电场单元71外加电场，使液滴产生分歧。当没有外加电场时，液滴进入下面的分支路14，当外加电场时，沿沟13进入溶液排出口31。当以上的液滴51移动时，也可以使基板11不倾斜，也可以通过调整沟12的宽度和深度控制移动速度。当然可以配置电极对该速度进行控制。
此外，例如，进行到分支路的沟14的液滴重新通过外加电场单元72、73而向沟15或沟16行进。然后，向沟15或沟16行进的液滴由于外加电场单元72或73而一边保持在该场中，一边与从溶液送入口22或23导入的液滴碰撞而融合。然后，在经过必要的反应时间后，液滴再次沿沟14或15滚动，用光学测定单元72、73测定后，在溶液排出口32、33处被回收。在该实施例中，将外加电场单元配置在基板上，但也可以如图1所示那样配置在防水基板表面的下面。此外，可以在溶液送入口21、22、23各处，导入外加了正电荷、负电荷的液滴，也可以导入不带电荷的液滴。此外，在该实施例中，在将多个液滴混合后，只进行光学测定，可以以光学测定的结果为基础对液滴进一步分离。
当然并不限于这些，在本发明申请中，对于其细节可以有各种各样的方式。
如以上详述的那样，根据本发明申请，可以以含有细胞或微生物、甚至化学物质等的微量液体为液滴进行操作、搬运、混合、观察、测定。

Claims (6)

1.液滴操作装置，其具有：表面具有实施了防水处理的绝缘层的基板、配置在该基板和该绝缘层之间的液滴移动或停止操作用的多个电极装置、向基板表面上供给液滴的装置、在基板表面上通过向上述电极装置外加电场而将液滴搬运的装置、测定液滴状态的装置。
2.权利要求1所述的液滴操作装置，其特征在于：具有用光学显微镜测定液滴的内部状态的装置。
3.权利要求1所述的液滴操作装置，其特征在于：具有测定液滴的吸光度的装置。
4.权利要求1所述的液滴操作装置，其特征在于：具有测定液滴的荧光强度的装置。
5.权利要求1所述的液滴操作装置，其特征在于：具有将多个液滴混合的装置。
6.权利要求1～5的任一项所述的液滴操作装置，其特征在于：液滴含有细胞或微生物。
CNB038201623A 2002-08-26 2003-08-26 液滴操作装置 Expired - Fee Related CN100520341C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP245901/2002 2002-08-26
JP2002245901A JP4031322B2 (ja) 2002-08-26 2002-08-26 液滴操作装置

Publications (2)

Publication Number Publication Date
CN1678896A CN1678896A (zh) 2005-10-05
CN100520341C true CN100520341C (zh) 2009-07-29

Family

ID=31944205

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB038201623A Expired - Fee Related CN100520341C (zh) 2002-08-26 2003-08-26 液滴操作装置

Country Status (5)

Country Link
US (1) US7597850B2 (zh)
EP (1) EP1541985A4 (zh)
JP (1) JP4031322B2 (zh)
CN (1) CN100520341C (zh)
WO (1) WO2004019005A1 (zh)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2266687A3 (en) 2003-04-10 2011-06-29 The President and Fellows of Harvard College Formation and control of fluidic species
JP4630870B2 (ja) 2003-08-27 2011-02-09 プレジデント アンド フェロウズ オブ ハーバード カレッジ 流体種の電子的制御
JP4773360B2 (ja) * 2003-11-17 2011-09-14 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 流体を操作するためのシステム
CA2696604A1 (en) 2007-08-24 2009-03-05 Advanced Liquid Logic, Inc. Bead manipulations on a droplet actuator
JP4906789B2 (ja) * 2008-06-12 2012-03-28 一般社団法人オンチップ・セロミクス・コンソーシアム 液滴分光システムと分光法
JP4574707B2 (ja) * 2008-10-20 2010-11-04 一般社団法人オンチップ・セロミクス・コンソーシアム 細胞分取及び培養方法とその装置
FR2975491B1 (fr) * 2011-05-19 2013-07-05 Commissariat Energie Atomique Procede d'identification de la phase d'un echantillon fluide dans un dispositif microfluidique.
JP2017020992A (ja) * 2015-07-15 2017-01-26 Jsr株式会社 分析方法
CN106198468B (zh) * 2016-06-30 2019-06-07 桂林理工大学 一种单液滴情况下电化学-荧光联用检测方法
CN109908987B (zh) * 2019-03-20 2021-01-29 山东大学 基于热释电效应无损失移取微量液滴的移液器的制备方法
JP7479606B2 (ja) * 2020-05-19 2024-05-09 国立研究開発法人産業技術総合研究所 放射性組成物の製造方法
CN115350733B (zh) * 2022-07-13 2023-12-22 中国科学院上海微系统与信息技术研究所 一种单层倾斜结构液滴存储腔的微流控芯片及其制备方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1363037A (zh) * 2000-01-25 2002-08-07 松下电器产业株式会社 使用生物传感器的测定装置以及其中使用的生物传感器、以及专用标准液

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5066512A (en) * 1989-12-08 1991-11-19 International Business Machines Corporation Electrostatic deposition of lcd color filters
US5849486A (en) * 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
US5486337A (en) * 1994-02-18 1996-01-23 General Atomics Device for electrostatic manipulation of droplets
JPH11218691A (ja) 1998-02-04 1999-08-10 Hitachi Ltd 液滴操作方法および装置
FI980874A (fi) * 1998-04-20 1999-10-21 Wallac Oy Menetelmä ja laite pienten nestemäärien kemiallisen analyysin suorittamiseksi
US6294063B1 (en) * 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
JP2001038246A (ja) 1999-08-02 2001-02-13 Japan Science & Technology Corp 生体微小成分の分離方法と分離装置
US6232072B1 (en) * 1999-10-15 2001-05-15 Agilent Technologies, Inc. Biopolymer array inspection
JP4093740B2 (ja) * 2001-09-27 2008-06-04 独立行政法人科学技術振興機構 微粒子分別マイクロチップと微粒子分別装置

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1363037A (zh) * 2000-01-25 2002-08-07 松下电器产业株式会社 使用生物传感器的测定装置以及其中使用的生物传感器、以及专用标准液

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ナノヘルスケアチップ. 一木隆范.BIO INDUSTRY,Vol.19 No.3. 2002
ナノヘルスケアチップ. 一木隆范.BIO INDUSTRY,Vol.19 No.3. 2002 *

Also Published As

Publication number Publication date
CN1678896A (zh) 2005-10-05
WO2004019005A1 (ja) 2004-03-04
EP1541985A4 (en) 2008-08-06
JP2004085322A (ja) 2004-03-18
US7597850B2 (en) 2009-10-06
EP1541985A1 (en) 2005-06-15
US20060039828A1 (en) 2006-02-23
JP4031322B2 (ja) 2008-01-09

Similar Documents

Publication Publication Date Title
CN100520341C (zh) 液滴操作装置
CN101848757B (zh) 用于改进的生物测定法的使用电场的方法和设备
US8703457B2 (en) Cell separation apparatus
CN114868006A (zh) 用于液滴操控的方法和系统
DE69312628T2 (de) Verfahren zum Fördern von Flüssigkeiten
US10576471B2 (en) Fluidics cartridge for use in the vertical or substantially vertical position
Ghallab et al. Sensing methods for dielectrophoresis phenomenon: from bulky instruments to lab-on-a-chip
CN101490562A (zh) 液体输送设备
KR20070035737A (ko) 전기습윤에서 액적의 접촉각 변위 및 변화속도 증가방법 및상기 방법에 의해 형성된 액적을 적용한 액적제어장치
CN109569458A (zh) 用于形成含极性介质的体积的阵列的装置
EP2662136A3 (en) Method for handling and mixing droplets
CN107855142A (zh) 一种基于微流控技术的检测芯片及检测设备
JP7500560B2 (ja) 細胞の微小液滴検出のためのデバイスおよび方法
CN101024336A (zh) 利用电荷浓度效应在基底上印刷生物分子滴的方法和装置
CN211771334U (zh) 一种核酸扩增反应检测装置
CN114887672B (zh) 一种基于介电泳及磁性捕获的微流控芯片及其控制设备
Galas et al. High Frequency Chemical Stimulation of Living Cells
Spencer et al. Ionflux: A Microfluidic Approach to Ensemble Recording and Block of Whole-Cell Current from Voltage-Gated Ion Channels
Hall et al. Capture & Release of Single Cells on a Microfluidic Chip Via Conical Nanopores
Xie et al. Nanoelectrodes for Neuron Recording and Stimulation
Sugino et al. 8-Parallel Bioparticle Sorter with a Multilayer PDMS Chip
Lin Dynamics of Charged Colloids in Nonpolar Solvents
CN117470922A (zh) 介电常数/电导率的测量方法、测量设备及标准品

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
ASS Succession or assignment of patent right

Owner name: YASUDA KENJI

Free format text: FORMER OWNER: INDEPENDENT ADMINISTRATIVE LEGAL PERSON S SCIENCE AND TECHNOLOGY DEVELOPMENT ORGANIZATION

Effective date: 20080530

C41 Transfer of patent application or patent right or utility model
TA01 Transfer of patent application right

Effective date of registration: 20080530

Address after: Tokyo, Japan

Applicant after: Yasuda Kenji

Address before: Saitama Prefecture, Japan

Applicant before: Independent Administrative Corporation Japan Science & Tech Corp.

C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20090729

Termination date: 20150826

EXPY Termination of patent right or utility model