CN100512823C - Microorganism feed addictive for improving sow reproductive performance and its preparation method - Google Patents
Microorganism feed addictive for improving sow reproductive performance and its preparation method Download PDFInfo
- Publication number
- CN100512823C CN100512823C CNB2005100290560A CN200510029056A CN100512823C CN 100512823 C CN100512823 C CN 100512823C CN B2005100290560 A CNB2005100290560 A CN B2005100290560A CN 200510029056 A CN200510029056 A CN 200510029056A CN 100512823 C CN100512823 C CN 100512823C
- Authority
- CN
- China
- Prior art keywords
- fermentation
- powder
- culture medium
- strain
- reproductive performance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000001850 reproductive effect Effects 0.000 title claims description 17
- 244000005700 microbiome Species 0.000 title description 5
- 238000002360 preparation method Methods 0.000 title description 2
- 239000000843 powder Substances 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 12
- 239000002131 composite material Substances 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 8
- 238000012216 screening Methods 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 4
- 238000000855 fermentation Methods 0.000 claims description 40
- 230000004151 fermentation Effects 0.000 claims description 40
- 239000001963 growth medium Substances 0.000 claims description 22
- 241000894006 Bacteria Species 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 15
- 230000003321 amplification Effects 0.000 claims description 13
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 13
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 12
- 239000000654 additive Substances 0.000 claims description 12
- 241000194108 Bacillus licheniformis Species 0.000 claims description 11
- 244000199866 Lactobacillus casei Species 0.000 claims description 11
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 11
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 11
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 11
- 230000000996 additive effect Effects 0.000 claims description 11
- 229940017800 lactobacillus casei Drugs 0.000 claims description 11
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 11
- 235000016709 nutrition Nutrition 0.000 claims description 11
- 210000000582 semen Anatomy 0.000 claims description 11
- 239000003795 chemical substances by application Substances 0.000 claims description 10
- 230000035764 nutrition Effects 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 8
- 238000009423 ventilation Methods 0.000 claims description 8
- 239000004202 carbamide Substances 0.000 claims description 6
- 235000013877 carbamide Nutrition 0.000 claims description 6
- 238000005516 engineering process Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- GVALZJMUIHGIMD-UHFFFAOYSA-H magnesium phosphate Chemical compound [Mg+2].[Mg+2].[Mg+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O GVALZJMUIHGIMD-UHFFFAOYSA-H 0.000 claims description 5
- 239000004137 magnesium phosphate Substances 0.000 claims description 5
- 229960002261 magnesium phosphate Drugs 0.000 claims description 5
- 229910000157 magnesium phosphate Inorganic materials 0.000 claims description 5
- 235000010994 magnesium phosphates Nutrition 0.000 claims description 5
- 235000013379 molasses Nutrition 0.000 claims description 5
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 4
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 4
- 238000009395 breeding Methods 0.000 claims description 4
- 230000001488 breeding effect Effects 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 3
- 235000013372 meat Nutrition 0.000 claims description 3
- 239000006916 nutrient agar Substances 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 235000014347 soups Nutrition 0.000 claims description 3
- 235000017550 sodium carbonate Nutrition 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 238000002156 mixing Methods 0.000 abstract description 2
- 241000186660 Lactobacillus Species 0.000 abstract 2
- 229940039696 lactobacillus Drugs 0.000 abstract 2
- 235000019737 Animal fat Nutrition 0.000 abstract 1
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 abstract 1
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 235000007164 Oryza sativa Nutrition 0.000 abstract 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract 1
- 235000013351 cheese Nutrition 0.000 abstract 1
- 239000000583 progesterone congener Substances 0.000 abstract 1
- 235000009566 rice Nutrition 0.000 abstract 1
- 239000002893 slag Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 description 12
- 210000001161 mammalian embryo Anatomy 0.000 description 9
- 229930182490 saponin Natural products 0.000 description 7
- 150000007949 saponins Chemical class 0.000 description 7
- 235000017709 saponins Nutrition 0.000 description 7
- 235000001014 amino acid Nutrition 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- 230000016087 ovulation Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 150000004676 glycans Chemical class 0.000 description 5
- 210000002381 plasma Anatomy 0.000 description 5
- 229920001184 polypeptide Polymers 0.000 description 5
- 229920001282 polysaccharide Polymers 0.000 description 5
- 239000005017 polysaccharide Substances 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 241001597008 Nomeidae Species 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 238000002513 implantation Methods 0.000 description 4
- 230000000968 intestinal effect Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000002207 metabolite Substances 0.000 description 4
- 150000007524 organic acids Chemical class 0.000 description 4
- 235000002378 plant sterols Nutrition 0.000 description 4
- 244000144977 poultry Species 0.000 description 4
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 3
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 3
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 3
- 239000013543 active substance Substances 0.000 description 3
- 230000003064 anti-oxidating effect Effects 0.000 description 3
- 229930003944 flavone Natural products 0.000 description 3
- 150000002212 flavone derivatives Chemical class 0.000 description 3
- 235000011949 flavones Nutrition 0.000 description 3
- 229910052733 gallium Inorganic materials 0.000 description 3
- 229930182470 glycoside Natural products 0.000 description 3
- 150000002338 glycosides Chemical class 0.000 description 3
- 229910052742 iron Inorganic materials 0.000 description 3
- 229910052748 manganese Inorganic materials 0.000 description 3
- 239000006041 probiotic Substances 0.000 description 3
- 235000018291 probiotics Nutrition 0.000 description 3
- 230000000630 rising effect Effects 0.000 description 3
- 239000003270 steroid hormone Substances 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 3
- 229910052725 zinc Inorganic materials 0.000 description 3
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 2
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 108010007622 LDL Lipoproteins Proteins 0.000 description 2
- 102000007330 LDL Lipoproteins Human genes 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 244000144987 brood Species 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000002124 endocrine Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 230000006651 lactation Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 230000000757 progestagenic effect Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 229960002477 riboflavin Drugs 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 208000006399 Premature Obstetric Labor Diseases 0.000 description 1
- 206010036600 Premature labour Diseases 0.000 description 1
- 102000003946 Prolactin Human genes 0.000 description 1
- 108010057464 Prolactin Proteins 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- 229930003471 Vitamin B2 Natural products 0.000 description 1
- 229930003537 Vitamin B3 Natural products 0.000 description 1
- 229930003571 Vitamin B5 Natural products 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 235000011054 acetic acid Nutrition 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 235000019728 animal nutrition Nutrition 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000037358 bacterial metabolism Effects 0.000 description 1
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 description 1
- 229960002079 calcium pantothenate Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000013530 defoamer Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000001842 enterocyte Anatomy 0.000 description 1
- 230000012173 estrus Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000008175 fetal development Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 239000003163 gonadal steroid hormone Substances 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000001983 lactogenic effect Effects 0.000 description 1
- 108010022197 lipoprotein cholesterol Proteins 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- DUWWHGPELOTTOE-UHFFFAOYSA-N n-(5-chloro-2,4-dimethoxyphenyl)-3-oxobutanamide Chemical compound COC1=CC(OC)=C(NC(=O)CC(C)=O)C=C1Cl DUWWHGPELOTTOE-UHFFFAOYSA-N 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- DFPAKSUCGFBDDF-UHFFFAOYSA-N nicotinic acid amide Natural products NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- CCHNOBQMQBSRHQ-UHFFFAOYSA-N phosphoric acid;7h-purin-6-amine Chemical compound OP(O)(O)=O.NC1=NC=NC2=C1NC=N2 CCHNOBQMQBSRHQ-UHFFFAOYSA-N 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 208000026440 premature labor Diseases 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 229940097325 prolactin Drugs 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 229930003799 tocopherol Natural products 0.000 description 1
- 229960001295 tocopherol Drugs 0.000 description 1
- 239000011732 tocopherol Substances 0.000 description 1
- 235000010384 tocopherol Nutrition 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 235000019160 vitamin B3 Nutrition 0.000 description 1
- 239000011708 vitamin B3 Substances 0.000 description 1
- 235000009492 vitamin B5 Nutrition 0.000 description 1
- 239000011675 vitamin B5 Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 1
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses an animal-fat gestagen and preparing method, which comprises the following steps: proceeding composite culture for lichen bacillus, plant lactobacillus, cheese lactobacillus and beer yeast, expanding culture liquid, proceeding aerobic ferment with culture base and sterilized water, centrifuging the ferment liquid at high speed, blending the ferment liquid with protein powder and rice slag evenly, placing in the sealed ferment chamber to proceed anaerobic ferment, drying at low temperature rapidly, grinding, screening.
Description
Technical field
The present invention relates to a kind of additive for microbe feedstuff and manufacture method that improves sow reproductive performance, can be used to influence reproductive capability and the state of sow, reach and improve ovulation quality, the early stage implantation rate of embryo and survival rate of embryo, produce the young number of living, belong to the agro-ecology product technique field thereby improve.
Background technology
At present, the patent of having applied for, application number is CN02136360, notification number is CN1471847, be entitled as Glyptal composition and its production and application, it is characterized in that: microorganism fungus kind is made fermentation culture behind liquid culture, fermented product carries out the nuclease hydrolysis after with the alkali extracting and makes nucleotide; To use protease hydrolysis after the fragmentation of animal internal organs, the homogenate, heat treatment, supernatant after filtration, dialysis back freeze concentration, make the cytoactive polypeptide; The two mixes by a certain percentage.This Glyptal composition only is the simple mixing that two kinds of commonsense methods prepare product, does not have outstanding novel part on technology.
Summary of the invention
The purpose of this invention is to provide a kind of additive for microbe feedstuff and manufacture method that improves sow reproductive performance, it is become to be grouped into by various active, contain protein, aminoacid, also contain the mushroom polysaccharide, Fe, Ga, Zn, nutrient substance such as Mn, as flavone and glycoside and organic acid, polypeptide, Saponin, plant sterol, some metabolites such as three obedient Saponins, these materials are absorbed the back by changing part plasma parameters concentration, make steroid hormone level rising and stable, influence reproductive capability and the state of sow, reach and improve the ovulation quality, factors such as early stage implantation rate of embryo and survival rate of embryo are produced the young number of living thereby significantly improved.
For realizing above purpose, technical scheme of the present invention provides a kind of additive for microbe feedstuff that improves sow reproductive performance, it is characterized in that, prepare by following weight percentages before the first order fermentation: composite bacteria agent capable amplification culture liquid 3-18%, culture medium 5-20%, water 62-92%
Described strain expanded culture liquid is made by Bacillus licheniformis, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum, and its weight percent proportioning is: Bacillus licheniformis 25-45%, Lactobacillus plantarum 15-30%, lactobacillus casei 0-20%, cereuisiae fermentum 20-40%;
Second level fermentation is prepared by following weight percentages:
Centrifugal back solid content 10-18%, nutrition carrier 80-95% after the first order fermentation;
Described culture medium is at least four kinds of mixture in bean cake powder, carbamide, ammonium sulfate, Testa Tritici, yeast powder, Semen Maydis pulp, Semen Maydis powder, molasses, sodium carbonate, magnesium phosphate, magnesium sulfate, the sterilized water, and its weight percent proportioning is: bean cake 2-6%, Testa Tritici 0-5%, carbamide 0-1%, ammonium sulfate 0-1%, yeast powder 0-3%, Semen Maydis pulp 0-1.5%, Semen Maydis powder 3-10%, molasses 1-3%, sodium carbonate 0-1%, magnesium phosphate 0-1%, magnesium sulfate 0-1%, sterilized water 85-94%.Described nutrition carrier is made up of bran powder, egg albumen powder, and its weight percent proportioning is: bran powder 90-94%, egg albumen powder 5-11%.
The strain that the preparation method of the additive for microbe feedstuff of raising sow reproductive performance adopts all comes by screening and optimizing, adopts two-stage fermentation technology simultaneously, and it comprises the following steps:
One. first order fermentation
The first step. the Bacillus licheniformis that will screen, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum strain cultured method in same amplification culture base is carried out preferably;
Second goes on foot. in the meat soup culture medium of respectively strain being transferred, after cultivating 22-26 hours under 35-40 ℃ of temperature, preserve after the separation that the selection colony characteristics is obviously, the vigor of examining under a microscope is strong and the fast bacterium colony of growth and breeding is rule;
The 3rd step. strain transferred Nutrient agar is housed does under 35-40 ℃ of temperature, to cultivate 45-50 hours in the Fructus Solani melongenae bottle of culture medium, put into 2-6 ℃ refrigerator then and preserve;
The 4th step. Bacillus licheniformis, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum are inoculated in respectively in proportion through in the culture medium of steam sterilization, putting into blender stirs, speed of agitator is 200r/min-240r/min, cultivation temperature is 35-40 ℃, ventilation 1:0.6, fermentation time is 22-26 hours, obtains composite bacteria agent capable amplification culture liquid;
The 5th step. composite bacteria agent capable amplification culture liquid proportionally is inoculated in the culture medium, mixes, speed of agitator is 200r/min-240r/min, and cultivation temperature is 35-40 ℃, and ventilation 1:0.6, fermentation time are 34-38 hours;
The 6th step. after the fermentation ends, screw settling centrifuge and the rotating speed that with fermentation liquid by rotating speed is 2800r/min-3200r/min is that the disk centrifugal separator of 6800r/min-7200r/min separates;
Two. second level fermentation
The 7th step. will add in the carrier according to the ratio of 1:6 from going out thing, put into high-speed mixer and mix, and insert in the Plastic Drum of sealing behind the mix homogeneously again, the fermenting house of putting into temperature and be 35-40 ℃ fermented 5-9 days;
The 8th the step. the product after the fermentation ends, put into boiling drier and carry out drying, with moisture Control in 12%;
The 9th step. with dried product, carry out crushing screening after, promptly obtain pulverous rich poultry progestogenic health finished product.
The present invention adopts and is mixed with organic nutrient substance by the multiple strain that filters out, make by the classification microbial fermentation technology, culture medium and nutrition carrier are as nutrient substance, after part is utilized by thalline, the enzyme that produces by bacterial metabolism decomposes the organic substance in the culture medium, and combination under given conditions, can form the purpose product, in culture, contain rich in protein, aminoacid, vitamin also contains the mushroom polysaccharide, Fe, Ga, Zn, nutrient substance such as Mn and as flavone and glycoside and organic acid, polypeptide, Saponin, plant sterol, some metabolites such as three obedient Saponins.
The amount of biological active substances such as vitamins and FAD is more among the present invention, and these chemical compounds all have antioxidation.Under multiple composition synergism, bring into play powerful antioxidation and render a service.Amino acid content height, especially proline simultaneously is from the composition of DNA in the metabolism of yeasts process.In addition, histidine is that 18 kinds of important amino acids almost all contain from the product of special material after fermentation, and these compositions are also significant aspect antioxidation.
Multiple beneficial microorganism among the present invention in conjunction with original probiotics, forms the superior microorganism flora rapidly after entering the sow intestinal, suppressed growth of harmful intestinal tract bacteria, has created a stable environment to the growth promoter of poultry and aquatic products; By a large amount of beneficial microorganism effects, active substance in the feedstuff is converted into the various aminoacid that absorbed by animal easily, organic acid, polysaccharide, multivitamin and antioxidant etc., make animal set up optimum little ecosystem, improved the combination of animal intestinal micropopulation, suppress the growth of intestinal corruptibility antibacterial, promote the Animal nutrition absorption function, ensured the nutritional need of sow at each physiological stage.
Multivitamin among the present invention also has special function for improving the sow fertility.Vitamin E also claims tocopherol (tocophero 1), mainly act on the stimulation antepituitary, promotion secretes sex hormones, and regulates the growth and the function of gonad, helps oestrusing, ovulate, become pregnant and embryo nidation, preventing to miscarry, improve the reproduction function of sow of sow; And vitamin B1 can prevent that premature labor from appearring in brood sow, farrowing rate is hanged down phenomenons such as reaching newborn piglet mortality rate height, vitamin B2 (riboflavin) can promote reproduction and the lactogenic function of pig, vitamin B3 (pantothenic acid) can promote sow to become pregnant and growth in piglets, and adenine phosphate (choline) can be regulated sow reproductive capability and endocrine at benign state.Vitamin B5 can promote the sow conception rate to rise, ovulation increases etc.; Vitamin B12 can improve sow conception rate and litter size, promotion piglet vigor etc.
Probiotic bacteria, aminoacid, vitamin not only are rich in the present invention, also contain trace element and other nutrient substance such as mushroom polysaccharide, Fe, Ga, Zn, Mn, as some metabolites such as flavone and glycoside thereof and organic acid, polypeptide, Saponin, plant sterol, three obedient Saponins, pass through to change part plasma parameters concentration after these material absorbing, make steroid hormone level rising and stable, influence reproductive capability and the state of sow, reach and improve ovulation quality, the early stage implantation rate of embryo and survival rate of embryo, produce the young number of living thereby significantly improved.
Under the consistent relatively situation of brood sow diets of different nutrition level, adding the additive for microbe feedstuff effect is to promote nutrition digestion to absorb, making blood plasma general volatile fatty acid, acetic acid, propanoic acid and butyro-concentration be linear increases, what produce helps reducing serum total cholesterol and low density lipoprotein, LDL content, improve this low cholesterol effect of hdl concentration, make the source of parents nutrition supply more can satisfy the needs of fetal development, thereby have influence on the piglet birth weight nutrition.Butanoic acid is the requisite energy of enterocyte, can promote piglet digestive system (intestinal villus) to grow, and the absorbing surface of the material that has additional nutrients makes the piglet lifelong benefit.
The height of lactation performance of lactating sows has crucial effects to the influence of suckling pig growth and development, and the present invention can improve sow lactation amount and breast milk quality, also may have influence on the prolactin antagonist secretion level by changing plasma parameters concentration; The directly synthetic butterfat of the acetate that can promote the daily ration fiber to produce.
The multiple thalline that contains among the present invention is synthetic, metabolite etc., has other additives special efficacy can't be obtained.As: the derivant of polypeptide is an antibody; Polysaccharide has anticoagulation, detoxifcation and immunization; The derivant of Saponin has antibacterial and anti-inflammation functions; Plant sterol and derivant thereof then have the effect of regulating poultry organism metabolism function and promoting its physiological function, guarantee that body is in production status in good health, thereby have improved the level of production.
Advantage of the present invention is:
1. adopt the probiotic bacteria symbiosis compatibility design concept of original creation, form by strain structure with specific purpose.
2. the trace element among the present invention all exists with the form of derivant, and it can give birth to free radical to body disease cell selective real estate, thereby kills and wounds sick cell.
3. after the biological active substances among the present invention is absorbed by body, by changing part plasma parameters concentration, make steroid hormone level rising and stable, influence reproductive capability and the state of sow, reach purposes such as improving ovulation quality, the early stage implantation rate of embryo and survival rate of embryo.
4. shorten sow and return the feelings phase, and it is neat to oestrus.
5. regulate sow reproductive capability and endocrine and be in benign state, promote the sow ovulation, improve its conception rate.
6. significantly improve number born of sow and produce the number of living, promote growth in piglets.
7. the use of alternative hormone or other medicines of promoting the sexual maturity ensures breeding production safety, pollution-free.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail:
Embodiment 1
Improve the additive for microbe feedstuff and the manufacture method of sow reproductive performance, the strain of employing all comes by screening and optimizing, adopts two-stage fermentation technology simultaneously, and it comprises the following steps:
One. first order fermentation
The first step. the Bacillus licheniformis that will screen, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum cultured method in same amplification culture base is carried out preferably;
Second step. in the meat soup culture medium of respectively strain being transferred, after 24 hours, preserve after the separation that the selection colony characteristics is obviously, the vigor of examining under a microscope is strong and the fast bacterium colony of growth and breeding is rule in cultivation under 37 ℃ of temperature;
The 3rd step. strain transferred Nutrient agar is housed does in the Fructus Solani melongenae bottle of culture medium, under 37 ℃ of temperature, cultivated 48 hours, put into 4 ℃ refrigerator then and preserve;
The 4th step. Bacillus licheniformis, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum are inoculated in respectively through in the culture medium of steam sterilization according to 35%, 20%, 15%, 30% ratio, speed of agitator 220r/min, 35 ℃ of cultivation temperature, ventilation 1:0.6, behind the fermentation time 24h, obtain composite bacteria agent capable amplification culture liquid;
The 5th step. composite bacteria agent capable amplification culture liquid is inoculated in the culture medium, and ratio is: strain expanded culture liquid 12%, culture medium 10%, sterilized water 78% mix, speed of agitator 220r/min, 35 ℃ of cultivation temperature, ventilation 1:0.6, fermentation time 36h;
Take by weighing fermentation medium by following weight proportion:
Soybean cake powder 2.5%, Semen Maydis powder 4%, yeast powder 0.8%, carbamide 0.05%, magnesium phosphate 0.3%, infusion 0.1%, 92.25%, 121 ℃ of steam sterilization 30min of water, the back pH value that disappears is 6.8;
The 6th step. after the fermentation ends, screw settling centrifuge and the rotating speed that with fermentation liquid by rotating speed is 3000r/min is that the disk centrifugal separator of 7000r/min separates;
Two. second level fermentation
The 7th step. will add according to the ratio of 1:6 and contain in the carrier of bran powder 85%, egg albumen powder 15% from going out thing, and put into high-speed mixer and mix, and insert in the Plastic Drum of sealing behind the mix homogeneously again, the fermenting house of putting into temperature and be 35 ℃ ferments 7 days;
The 8th the step. the product after the fermentation ends, put into boiling drier and carry out drying, with moisture Control in 12%;
The 9th step. with dried product, carry out crushing screening after, promptly obtain pulverous rich poultry progestogenic health finished product.
Embodiment 2:
One. in the first order fermentation
The 4th step. Bacillus licheniformis, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum are inoculated in respectively through in the culture medium of steam sterilization according to 40%, 15%, 10%, 35% ratio, speed of agitator 220r/min, 35 ℃ of cultivation temperature, ventilation 1:0.6, behind the fermentation time 24h, obtain composite bacteria agent capable amplification culture liquid;
The 5th step. composite bacteria agent capable amplification culture liquid is inoculated in the culture medium, and ratio is: strain expanded culture liquid 14%, culture medium 12%, sterilized water 74% mix, speed of agitator 220r/min, cultivation temperature 35 degree, ventilation 1:0.6, fermentation time 32h;
Take by weighing fermentation medium by following weight proportion:
Soybean cake powder 3%, Semen Maydis powder 5%, Semen Maydis pulp 0.5%, yeast powder 1.0%, carbamide 0.05%, molasses 0.4%, potassium dihydrogen phosphate 0.05%, sodium hydrogen phosphate 0.4%, defoamer 0.1%, water 89.5%, 121 ℃ of steam sterilization 30min, the back pH value that disappears is 6.8;
Two. in the fermentation of the second level
The 7th step. the ratio adding of centrifugal back solid content according to solid content 10-18%, nutrition carrier 80-95% contained in the carrier of bran powder 92%, egg albumen powder 8%, putting into high-speed mixer mixes, insert again in the Plastic Drum of sealing behind the mix homogeneously, put into temperature and be 35 ℃ fermenting house fermentation 7 days;
Other steps are identical with embodiment 1.
Claims (4)
1. an additive for microbe feedstuff that improves sow reproductive performance is characterized in that, is prepared by following weight percentages before the first order fermentation: composite bacteria agent capable amplification culture liquid 3-18%, culture medium 5-20%, water 62-92%,
Described strain expanded culture liquid is made by Bacillus licheniformis, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum, and its weight percent proportioning is: Bacillus licheniformis 25-45%, Lactobacillus plantarum 15-30%, lactobacillus casei 0-20%, cereuisiae fermentum 20-40%;
Second level fermentation is prepared by following weight percentages:
Centrifugal back solid content 10-18%, nutrition carrier 80-95% after the first order fermentation.
2. the additive for microbe feedstuff of raising sow reproductive performance according to claim 1, it is characterized in that, described culture medium is a bean cake powder, carbamide, ammonium sulfate, Testa Tritici, yeast powder, Semen Maydis pulp, Semen Maydis powder, molasses, sodium carbonate, magnesium phosphate, magnesium sulfate, in the sterilized water at least four kinds, its weight percent proportioning is: bean cake 2-6%, Testa Tritici 0-5%, carbamide 0-1%, ammonium sulfate 0-1%, yeast powder 0-3%, Semen Maydis pulp 0-1.5%, Semen Maydis powder 3-10%, molasses 1-3%, sodium carbonate 0-1%, magnesium phosphate 0-1%, magnesium sulfate 0-1%, sterilized water 85-94%.
3. the additive for microbe feedstuff of raising sow reproductive performance according to claim 1 is characterized in that, described nutrition carrier is made up of bran powder, egg albumen powder, and its weight percent proportioning is: bran powder 90-94%, egg albumen powder 5-11%.
4. the manufacture method of the additive for microbe feedstuff of raising sow reproductive performance according to claim 1 is characterized in that, the strain of employing all comes by screening and optimizing, adopts two-stage fermentation technology simultaneously, and it comprises the following steps:
One. first order fermentation
The first step. the Bacillus licheniformis that will screen, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum strain cultured method in same amplification culture base is carried out preferably;
Second goes on foot. in the meat soup culture medium of respectively strain being transferred, after cultivating 22-26 hours under 35-40 ℃ of temperature, preserved after the separation that the selection colony characteristics is obviously, the vigor of examining under a microscope is strong and the fast bacterium colony of growth and breeding is rule;
The 3rd step. strain transferred Nutrient agar is housed does under 35-40 ℃ of temperature, to cultivate 45-50 hours in the Fructus Solani melongenae bottle of culture medium, put into 2-6 ℃ refrigerator then and preserve;
The 4th step. Bacillus licheniformis, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum are inoculated in respectively in proportion through in the culture medium of steam sterilization, putting into blender stirs, speed of agitator is 200r/min-240r/min, cultivation temperature is 35-40 ℃, ventilation 1:0.6, fermentation time is 22-26 hours, obtains composite bacteria agent capable amplification culture liquid;
The 5th step. composite bacteria agent capable amplification culture liquid proportionally is inoculated in the culture medium, mixes, speed of agitator is 200r/min-240r/min, and cultivation temperature is 35-40 ℃, and ventilation 1:0.6, fermentation time are 34-38 hours;
The 6th step. after the fermentation ends, screw settling centrifuge and the rotating speed that with fermentation liquid by rotating speed is 2800r/min-3200r/min is that the disk centrifugal separator of 6800r/min-7200r/min separates;
Two. second level fermentation
The 7th step. will add in the carrier according to the ratio of 1:6 from going out thing, put into high-speed mixer and mix, and insert in the Plastic Drum of sealing behind the mix homogeneously again, the fermenting house of putting into temperature and be 35-40 ℃ fermented 5-9 days;
The 8th the step. the product after the fermentation ends, put into boiling drier and carry out drying, with moisture Control in 12%;
The 9th the step. with dried product, carry out crushing screening after, promptly obtain pulverous finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100290560A CN100512823C (en) | 2005-08-24 | 2005-08-24 | Microorganism feed addictive for improving sow reproductive performance and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100290560A CN100512823C (en) | 2005-08-24 | 2005-08-24 | Microorganism feed addictive for improving sow reproductive performance and its preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1919212A CN1919212A (en) | 2007-02-28 |
| CN100512823C true CN100512823C (en) | 2009-07-15 |
Family
ID=37777148
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100290560A Expired - Fee Related CN100512823C (en) | 2005-08-24 | 2005-08-24 | Microorganism feed addictive for improving sow reproductive performance and its preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100512823C (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101199321B (en) * | 2006-12-14 | 2012-10-24 | 上海亘卓生物工程有限公司 | Feed-stuff additive for improving meat quality of avian fed with microorganism and its preparation method |
| CN101558821B (en) * | 2009-06-09 | 2012-07-04 | 刘键 | Animal feed and preparation method thereof |
| CN102696916A (en) * | 2012-06-02 | 2012-10-03 | 上海创博饲料科技有限公司 | Compound fermentation method for producing fermented feed |
| CN102783585B (en) * | 2012-08-30 | 2014-04-16 | 山东新希望六和集团有限公司 | Feed for improving reproductive performance of sows |
| CN105724762A (en) * | 2016-02-26 | 2016-07-06 | 安徽天邦生物技术有限公司 | Biologically fermented sow feed and preparation method thereof |
| CN111109442A (en) * | 2019-12-31 | 2020-05-08 | 四川铁骑力士实业有限公司 | Compound feed for pregnant sows of Sichuan-Tibet black pigs and using method of compound feed |
| CN113265349B (en) * | 2021-04-30 | 2022-06-28 | 华中农业大学 | Microecological preparation for increasing mature follicle number and litter size of mammals and preparation method and application thereof |
| CN113575789A (en) * | 2021-08-02 | 2021-11-02 | 四川成康动物药业有限公司 | Traditional Chinese medicine fermentation product for improving pig reproductive performance and preparation method and preparation device thereof |
| CN113729122B (en) * | 2021-09-03 | 2023-07-25 | 邦基（山东）农业科技有限公司 | Feed capable of improving reproductive performance of sow production and preparation method thereof |
-
2005
- 2005-08-24 CN CNB2005100290560A patent/CN100512823C/en not_active Expired - Fee Related
Non-Patent Citations (6)
| Title |
|---|
| 微生物饲料添加剂. 赵献军.陕西农业科学，第2期. 2002 |
| 微生物饲料添加剂. 赵献军.陕西农业科学，第2期. 2002 * |
| 猪促生长添加剂的研究进展. 沈咏舟等.中国饲料，第6期. 1996 |
| 猪促生长添加剂的研究进展. 沈咏舟等.中国饲料，第6期. 1996 * |
| 饲用芽孢杆菌在当前畜牧业中的应用. 薛冬琳等.山东家禽，第12期. 2004 |
| 饲用芽孢杆菌在当前畜牧业中的应用. 薛冬琳等.山东家禽，第12期. 2004 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1919212A (en) | 2007-02-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100512823C (en) | Microorganism feed addictive for improving sow reproductive performance and its preparation method | |
| CN102232475A (en) | Physiological regulator for male animals and preparation method thereof | |
| CN106819383A (en) | It is a kind of to promote fermented feed of piglet growth and preparation method thereof | |
| CN108835444A (en) | Kind goose feed, preparation method, application and fowl food | |
| CN106954733A (en) | A kind of preparation method and applications of manioc waste activated feed raw material | |
| CN107683972A (en) | Improve fermented feed of milking sow production performance and its preparation method and application | |
| CN101999556B (en) | Special animal nutrient for beef cattle and preparation method thereof | |
| CN111513177A (en) | Preparation method of Chinese herbal medicine fermented feed | |
| CN104543410A (en) | Health disease-resistant pig feed additive and preparation method thereof | |
| CN108029853B (en) | Zymophyte liquid, product containing zymophyte liquid and applied to lactating sows, and preparation method and application of product | |
| CN106107074A (en) | A kind of powered milk substitute preventing lamb gastrointestinal tract flatulence and opened guideway and feeding method | |
| CN105994959A (en) | Preparation method of lactobacillus acidophilus feed additive | |
| CN108277188A (en) | A kind of weanling pig compound microbial culture starter and its application | |
| KR20000052237A (en) | A method of manufacturing a feed composite ,which contains yellow earth and chitosan, for livestock and cultured fish | |
| CN107094991A (en) | A kind of animal selenium-enriched health care nutrient solution, preparation method and application | |
| CN101748065A (en) | Production method for feeding monascus microbial preparations | |
| CN107198038B (en) | Chlorella powder and spirulina powder composition and feed prepared from composition and used for breeding healthy pigs | |
| CN101074420B (en) | Polypeptide and microbial preparation for improving ox myotendinous development and its production | |
| CN102640889A (en) | Detoxifying agent for cottonseed meal and rapeseed meal as well as preparation method thereof and preparation method of microorganism feed prepared by detoxifying agent | |
| CN109527268A (en) | A kind of production method of slow-release probiotics shrimp feed | |
| CN109645220A (en) | A kind of composite microbial feed additive and preparation method thereof | |
| CN109527238A (en) | A kind of preparation method of the piglet feed of enteric solubility compound micro-ecological preparation | |
| CN109393154A (en) | A kind of microorganism probiotic fermented feed | |
| CN108835421A (en) | A kind of ecology pannage and preparation method thereof | |
| CN108813106A (en) | A kind of fermentative feedstuff of microbe and its application method preventing and treating prevention of sow constipation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090715 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |