CN100512823C - Microorganism feed addictive for improving sow reproductive performance and its preparation method - Google Patents
Microorganism feed addictive for improving sow reproductive performance and its preparation method Download PDFInfo
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- CN100512823C CN100512823C CNB2005100290560A CN200510029056A CN100512823C CN 100512823 C CN100512823 C CN 100512823C CN B2005100290560 A CNB2005100290560 A CN B2005100290560A CN 200510029056 A CN200510029056 A CN 200510029056A CN 100512823 C CN100512823 C CN 100512823C
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- Fodder In General (AREA)
Abstract
The invention discloses an animal-fat gestagen and preparing method, which comprises the following steps: proceeding composite culture for lichen bacillus, plant lactobacillus, cheese lactobacillus and beer yeast, expanding culture liquid, proceeding aerobic ferment with culture base and sterilized water, centrifuging the ferment liquid at high speed, blending the ferment liquid with protein powder and rice slag evenly, placing in the sealed ferment chamber to proceed anaerobic ferment, drying at low temperature rapidly, grinding, screening.
Description
Technical field
The present invention relates to a kind of additive for microbe feedstuff and manufacture method that improves sow reproductive performance, can be used to influence reproductive capability and the state of sow, reach and improve ovulation quality, the early stage implantation rate of embryo and survival rate of embryo, produce the young number of living, belong to the agro-ecology product technique field thereby improve.
Background technology
At present, the patent of having applied for, application number is CN02136360, notification number is CN1471847, be entitled as Glyptal composition and its production and application, it is characterized in that: microorganism fungus kind is made fermentation culture behind liquid culture, fermented product carries out the nuclease hydrolysis after with the alkali extracting and makes nucleotide; To use protease hydrolysis after the fragmentation of animal internal organs, the homogenate, heat treatment, supernatant after filtration, dialysis back freeze concentration, make the cytoactive polypeptide; The two mixes by a certain percentage.This Glyptal composition only is the simple mixing that two kinds of commonsense methods prepare product, does not have outstanding novel part on technology.
Summary of the invention
The purpose of this invention is to provide a kind of additive for microbe feedstuff and manufacture method that improves sow reproductive performance, it is become to be grouped into by various active, contain protein, aminoacid, also contain the mushroom polysaccharide, Fe, Ga, Zn, nutrient substance such as Mn, as flavone and glycoside and organic acid, polypeptide, Saponin, plant sterol, some metabolites such as three obedient Saponins, these materials are absorbed the back by changing part plasma parameters concentration, make steroid hormone level rising and stable, influence reproductive capability and the state of sow, reach and improve the ovulation quality, factors such as early stage implantation rate of embryo and survival rate of embryo are produced the young number of living thereby significantly improved.
For realizing above purpose, technical scheme of the present invention provides a kind of additive for microbe feedstuff that improves sow reproductive performance, it is characterized in that, prepare by following weight percentages before the first order fermentation: composite bacteria agent capable amplification culture liquid 3-18%, culture medium 5-20%, water 62-92%
Described strain expanded culture liquid is made by Bacillus licheniformis, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum, and its weight percent proportioning is: Bacillus licheniformis 25-45%, Lactobacillus plantarum 15-30%, lactobacillus casei 0-20%, cereuisiae fermentum 20-40%;
Second level fermentation is prepared by following weight percentages:
Centrifugal back solid content 10-18%, nutrition carrier 80-95% after the first order fermentation;
Described culture medium is at least four kinds of mixture in bean cake powder, carbamide, ammonium sulfate, Testa Tritici, yeast powder, Semen Maydis pulp, Semen Maydis powder, molasses, sodium carbonate, magnesium phosphate, magnesium sulfate, the sterilized water, and its weight percent proportioning is: bean cake 2-6%, Testa Tritici 0-5%, carbamide 0-1%, ammonium sulfate 0-1%, yeast powder 0-3%, Semen Maydis pulp 0-1.5%, Semen Maydis powder 3-10%, molasses 1-3%, sodium carbonate 0-1%, magnesium phosphate 0-1%, magnesium sulfate 0-1%, sterilized water 85-94%.Described nutrition carrier is made up of bran powder, egg albumen powder, and its weight percent proportioning is: bran powder 90-94%, egg albumen powder 5-11%.
The strain that the preparation method of the additive for microbe feedstuff of raising sow reproductive performance adopts all comes by screening and optimizing, adopts two-stage fermentation technology simultaneously, and it comprises the following steps:
One. first order fermentation
The first step. the Bacillus licheniformis that will screen, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum strain cultured method in same amplification culture base is carried out preferably;
Second goes on foot. in the meat soup culture medium of respectively strain being transferred, after cultivating 22-26 hours under 35-40 ℃ of temperature, preserve after the separation that the selection colony characteristics is obviously, the vigor of examining under a microscope is strong and the fast bacterium colony of growth and breeding is rule;
The 3rd step. strain transferred Nutrient agar is housed does under 35-40 ℃ of temperature, to cultivate 45-50 hours in the Fructus Solani melongenae bottle of culture medium, put into 2-6 ℃ refrigerator then and preserve;
The 4th step. Bacillus licheniformis, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum are inoculated in respectively in proportion through in the culture medium of steam sterilization, putting into blender stirs, speed of agitator is 200r/min-240r/min, cultivation temperature is 35-40 ℃, ventilation 1:0.6, fermentation time is 22-26 hours, obtains composite bacteria agent capable amplification culture liquid;
The 5th step. composite bacteria agent capable amplification culture liquid proportionally is inoculated in the culture medium, mixes, speed of agitator is 200r/min-240r/min, and cultivation temperature is 35-40 ℃, and ventilation 1:0.6, fermentation time are 34-38 hours;
The 6th step. after the fermentation ends, screw settling centrifuge and the rotating speed that with fermentation liquid by rotating speed is 2800r/min-3200r/min is that the disk centrifugal separator of 6800r/min-7200r/min separates;
Two. second level fermentation
The 7th step. will add in the carrier according to the ratio of 1:6 from going out thing, put into high-speed mixer and mix, and insert in the Plastic Drum of sealing behind the mix homogeneously again, the fermenting house of putting into temperature and be 35-40 ℃ fermented 5-9 days;
The 8th the step. the product after the fermentation ends, put into boiling drier and carry out drying, with moisture Control in 12%;
The 9th step. with dried product, carry out crushing screening after, promptly obtain pulverous rich poultry progestogenic health finished product.
The present invention adopts and is mixed with organic nutrient substance by the multiple strain that filters out, make by the classification microbial fermentation technology, culture medium and nutrition carrier are as nutrient substance, after part is utilized by thalline, the enzyme that produces by bacterial metabolism decomposes the organic substance in the culture medium, and combination under given conditions, can form the purpose product, in culture, contain rich in protein, aminoacid, vitamin also contains the mushroom polysaccharide, Fe, Ga, Zn, nutrient substance such as Mn and as flavone and glycoside and organic acid, polypeptide, Saponin, plant sterol, some metabolites such as three obedient Saponins.
The amount of biological active substances such as vitamins and FAD is more among the present invention, and these chemical compounds all have antioxidation.Under multiple composition synergism, bring into play powerful antioxidation and render a service.Amino acid content height, especially proline simultaneously is from the composition of DNA in the metabolism of yeasts process.In addition, histidine is that 18 kinds of important amino acids almost all contain from the product of special material after fermentation, and these compositions are also significant aspect antioxidation.
Multiple beneficial microorganism among the present invention in conjunction with original probiotics, forms the superior microorganism flora rapidly after entering the sow intestinal, suppressed growth of harmful intestinal tract bacteria, has created a stable environment to the growth promoter of poultry and aquatic products; By a large amount of beneficial microorganism effects, active substance in the feedstuff is converted into the various aminoacid that absorbed by animal easily, organic acid, polysaccharide, multivitamin and antioxidant etc., make animal set up optimum little ecosystem, improved the combination of animal intestinal micropopulation, suppress the growth of intestinal corruptibility antibacterial, promote the Animal nutrition absorption function, ensured the nutritional need of sow at each physiological stage.
Multivitamin among the present invention also has special function for improving the sow fertility.Vitamin E also claims tocopherol (tocophero 1), mainly act on the stimulation antepituitary, promotion secretes sex hormones, and regulates the growth and the function of gonad, helps oestrusing, ovulate, become pregnant and embryo nidation, preventing to miscarry, improve the reproduction function of sow of sow; And vitamin B1 can prevent that premature labor from appearring in brood sow, farrowing rate is hanged down phenomenons such as reaching newborn piglet mortality rate height, vitamin B2 (riboflavin) can promote reproduction and the lactogenic function of pig, vitamin B3 (pantothenic acid) can promote sow to become pregnant and growth in piglets, and adenine phosphate (choline) can be regulated sow reproductive capability and endocrine at benign state.Vitamin B5 can promote the sow conception rate to rise, ovulation increases etc.; Vitamin B12 can improve sow conception rate and litter size, promotion piglet vigor etc.
Probiotic bacteria, aminoacid, vitamin not only are rich in the present invention, also contain trace element and other nutrient substance such as mushroom polysaccharide, Fe, Ga, Zn, Mn, as some metabolites such as flavone and glycoside thereof and organic acid, polypeptide, Saponin, plant sterol, three obedient Saponins, pass through to change part plasma parameters concentration after these material absorbing, make steroid hormone level rising and stable, influence reproductive capability and the state of sow, reach and improve ovulation quality, the early stage implantation rate of embryo and survival rate of embryo, produce the young number of living thereby significantly improved.
Under the consistent relatively situation of brood sow diets of different nutrition level, adding the additive for microbe feedstuff effect is to promote nutrition digestion to absorb, making blood plasma general volatile fatty acid, acetic acid, propanoic acid and butyro-concentration be linear increases, what produce helps reducing serum total cholesterol and low density lipoprotein, LDL content, improve this low cholesterol effect of hdl concentration, make the source of parents nutrition supply more can satisfy the needs of fetal development, thereby have influence on the piglet birth weight nutrition.Butanoic acid is the requisite energy of enterocyte, can promote piglet digestive system (intestinal villus) to grow, and the absorbing surface of the material that has additional nutrients makes the piglet lifelong benefit.
The height of lactation performance of lactating sows has crucial effects to the influence of suckling pig growth and development, and the present invention can improve sow lactation amount and breast milk quality, also may have influence on the prolactin antagonist secretion level by changing plasma parameters concentration; The directly synthetic butterfat of the acetate that can promote the daily ration fiber to produce.
The multiple thalline that contains among the present invention is synthetic, metabolite etc., has other additives special efficacy can't be obtained.As: the derivant of polypeptide is an antibody; Polysaccharide has anticoagulation, detoxifcation and immunization; The derivant of Saponin has antibacterial and anti-inflammation functions; Plant sterol and derivant thereof then have the effect of regulating poultry organism metabolism function and promoting its physiological function, guarantee that body is in production status in good health, thereby have improved the level of production.
Advantage of the present invention is:
1. adopt the probiotic bacteria symbiosis compatibility design concept of original creation, form by strain structure with specific purpose.
2. the trace element among the present invention all exists with the form of derivant, and it can give birth to free radical to body disease cell selective real estate, thereby kills and wounds sick cell.
3. after the biological active substances among the present invention is absorbed by body, by changing part plasma parameters concentration, make steroid hormone level rising and stable, influence reproductive capability and the state of sow, reach purposes such as improving ovulation quality, the early stage implantation rate of embryo and survival rate of embryo.
4. shorten sow and return the feelings phase, and it is neat to oestrus.
5. regulate sow reproductive capability and endocrine and be in benign state, promote the sow ovulation, improve its conception rate.
6. significantly improve number born of sow and produce the number of living, promote growth in piglets.
7. the use of alternative hormone or other medicines of promoting the sexual maturity ensures breeding production safety, pollution-free.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail:
Embodiment 1
Improve the additive for microbe feedstuff and the manufacture method of sow reproductive performance, the strain of employing all comes by screening and optimizing, adopts two-stage fermentation technology simultaneously, and it comprises the following steps:
One. first order fermentation
The first step. the Bacillus licheniformis that will screen, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum cultured method in same amplification culture base is carried out preferably;
Second step. in the meat soup culture medium of respectively strain being transferred, after 24 hours, preserve after the separation that the selection colony characteristics is obviously, the vigor of examining under a microscope is strong and the fast bacterium colony of growth and breeding is rule in cultivation under 37 ℃ of temperature;
The 3rd step. strain transferred Nutrient agar is housed does in the Fructus Solani melongenae bottle of culture medium, under 37 ℃ of temperature, cultivated 48 hours, put into 4 ℃ refrigerator then and preserve;
The 4th step. Bacillus licheniformis, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum are inoculated in respectively through in the culture medium of steam sterilization according to 35%, 20%, 15%, 30% ratio, speed of agitator 220r/min, 35 ℃ of cultivation temperature, ventilation 1:0.6, behind the fermentation time 24h, obtain composite bacteria agent capable amplification culture liquid;
The 5th step. composite bacteria agent capable amplification culture liquid is inoculated in the culture medium, and ratio is: strain expanded culture liquid 12%, culture medium 10%, sterilized water 78% mix, speed of agitator 220r/min, 35 ℃ of cultivation temperature, ventilation 1:0.6, fermentation time 36h;
Take by weighing fermentation medium by following weight proportion:
Soybean cake powder 2.5%, Semen Maydis powder 4%, yeast powder 0.8%, carbamide 0.05%, magnesium phosphate 0.3%, infusion 0.1%, 92.25%, 121 ℃ of steam sterilization 30min of water, the back pH value that disappears is 6.8;
The 6th step. after the fermentation ends, screw settling centrifuge and the rotating speed that with fermentation liquid by rotating speed is 3000r/min is that the disk centrifugal separator of 7000r/min separates;
Two. second level fermentation
The 7th step. will add according to the ratio of 1:6 and contain in the carrier of bran powder 85%, egg albumen powder 15% from going out thing, and put into high-speed mixer and mix, and insert in the Plastic Drum of sealing behind the mix homogeneously again, the fermenting house of putting into temperature and be 35 ℃ ferments 7 days;
The 8th the step. the product after the fermentation ends, put into boiling drier and carry out drying, with moisture Control in 12%;
The 9th step. with dried product, carry out crushing screening after, promptly obtain pulverous rich poultry progestogenic health finished product.
Embodiment 2:
One. in the first order fermentation
The 4th step. Bacillus licheniformis, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum are inoculated in respectively through in the culture medium of steam sterilization according to 40%, 15%, 10%, 35% ratio, speed of agitator 220r/min, 35 ℃ of cultivation temperature, ventilation 1:0.6, behind the fermentation time 24h, obtain composite bacteria agent capable amplification culture liquid;
The 5th step. composite bacteria agent capable amplification culture liquid is inoculated in the culture medium, and ratio is: strain expanded culture liquid 14%, culture medium 12%, sterilized water 74% mix, speed of agitator 220r/min, cultivation temperature 35 degree, ventilation 1:0.6, fermentation time 32h;
Take by weighing fermentation medium by following weight proportion:
Soybean cake powder 3%, Semen Maydis powder 5%, Semen Maydis pulp 0.5%, yeast powder 1.0%, carbamide 0.05%, molasses 0.4%, potassium dihydrogen phosphate 0.05%, sodium hydrogen phosphate 0.4%, defoamer 0.1%, water 89.5%, 121 ℃ of steam sterilization 30min, the back pH value that disappears is 6.8;
Two. in the fermentation of the second level
The 7th step. the ratio adding of centrifugal back solid content according to solid content 10-18%, nutrition carrier 80-95% contained in the carrier of bran powder 92%, egg albumen powder 8%, putting into high-speed mixer mixes, insert again in the Plastic Drum of sealing behind the mix homogeneously, put into temperature and be 35 ℃ fermenting house fermentation 7 days;
Other steps are identical with embodiment 1.
Claims (4)
1. an additive for microbe feedstuff that improves sow reproductive performance is characterized in that, is prepared by following weight percentages before the first order fermentation: composite bacteria agent capable amplification culture liquid 3-18%, culture medium 5-20%, water 62-92%,
Described strain expanded culture liquid is made by Bacillus licheniformis, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum, and its weight percent proportioning is: Bacillus licheniformis 25-45%, Lactobacillus plantarum 15-30%, lactobacillus casei 0-20%, cereuisiae fermentum 20-40%;
Second level fermentation is prepared by following weight percentages:
Centrifugal back solid content 10-18%, nutrition carrier 80-95% after the first order fermentation.
2. the additive for microbe feedstuff of raising sow reproductive performance according to claim 1, it is characterized in that, described culture medium is a bean cake powder, carbamide, ammonium sulfate, Testa Tritici, yeast powder, Semen Maydis pulp, Semen Maydis powder, molasses, sodium carbonate, magnesium phosphate, magnesium sulfate, in the sterilized water at least four kinds, its weight percent proportioning is: bean cake 2-6%, Testa Tritici 0-5%, carbamide 0-1%, ammonium sulfate 0-1%, yeast powder 0-3%, Semen Maydis pulp 0-1.5%, Semen Maydis powder 3-10%, molasses 1-3%, sodium carbonate 0-1%, magnesium phosphate 0-1%, magnesium sulfate 0-1%, sterilized water 85-94%.
3. the additive for microbe feedstuff of raising sow reproductive performance according to claim 1 is characterized in that, described nutrition carrier is made up of bran powder, egg albumen powder, and its weight percent proportioning is: bran powder 90-94%, egg albumen powder 5-11%.
4. the manufacture method of the additive for microbe feedstuff of raising sow reproductive performance according to claim 1 is characterized in that, the strain of employing all comes by screening and optimizing, adopts two-stage fermentation technology simultaneously, and it comprises the following steps:
One. first order fermentation
The first step. the Bacillus licheniformis that will screen, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum strain cultured method in same amplification culture base is carried out preferably;
Second goes on foot. in the meat soup culture medium of respectively strain being transferred, after cultivating 22-26 hours under 35-40 ℃ of temperature, preserved after the separation that the selection colony characteristics is obviously, the vigor of examining under a microscope is strong and the fast bacterium colony of growth and breeding is rule;
The 3rd step. strain transferred Nutrient agar is housed does under 35-40 ℃ of temperature, to cultivate 45-50 hours in the Fructus Solani melongenae bottle of culture medium, put into 2-6 ℃ refrigerator then and preserve;
The 4th step. Bacillus licheniformis, Lactobacillus plantarum, lactobacillus casei, cereuisiae fermentum are inoculated in respectively in proportion through in the culture medium of steam sterilization, putting into blender stirs, speed of agitator is 200r/min-240r/min, cultivation temperature is 35-40 ℃, ventilation 1:0.6, fermentation time is 22-26 hours, obtains composite bacteria agent capable amplification culture liquid;
The 5th step. composite bacteria agent capable amplification culture liquid proportionally is inoculated in the culture medium, mixes, speed of agitator is 200r/min-240r/min, and cultivation temperature is 35-40 ℃, and ventilation 1:0.6, fermentation time are 34-38 hours;
The 6th step. after the fermentation ends, screw settling centrifuge and the rotating speed that with fermentation liquid by rotating speed is 2800r/min-3200r/min is that the disk centrifugal separator of 6800r/min-7200r/min separates;
Two. second level fermentation
The 7th step. will add in the carrier according to the ratio of 1:6 from going out thing, put into high-speed mixer and mix, and insert in the Plastic Drum of sealing behind the mix homogeneously again, the fermenting house of putting into temperature and be 35-40 ℃ fermented 5-9 days;
The 8th the step. the product after the fermentation ends, put into boiling drier and carry out drying, with moisture Control in 12%;
The 9th the step. with dried product, carry out crushing screening after, promptly obtain pulverous finished product.
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CN101199321B (en) * | 2006-12-14 | 2012-10-24 | 上海亘卓生物工程有限公司 | Feed-stuff additive for improving meat quality of avian fed with microorganism and its preparation method |
CN101558821B (en) * | 2009-06-09 | 2012-07-04 | 刘键 | Animal feed and preparation method thereof |
CN102696916A (en) * | 2012-06-02 | 2012-10-03 | 上海创博饲料科技有限公司 | Compound fermentation method for producing fermented feed |
CN102783585B (en) * | 2012-08-30 | 2014-04-16 | 山东新希望六和集团有限公司 | Feed for improving reproductive performance of sows |
CN105724762A (en) * | 2016-02-26 | 2016-07-06 | 安徽天邦生物技术有限公司 | Biologically fermented sow feed and preparation method thereof |
CN111109442A (en) * | 2019-12-31 | 2020-05-08 | 四川铁骑力士实业有限公司 | Compound feed for pregnant sows of Sichuan-Tibet black pigs and using method of compound feed |
CN113265349B (en) * | 2021-04-30 | 2022-06-28 | 华中农业大学 | Microecological preparation for increasing mature follicle number and litter size of mammals and preparation method and application thereof |
CN113575789A (en) * | 2021-08-02 | 2021-11-02 | 四川成康动物药业有限公司 | Traditional Chinese medicine fermentation product for improving pig reproductive performance and preparation method and preparation device thereof |
CN113729122B (en) * | 2021-09-03 | 2023-07-25 | 邦基(山东)农业科技有限公司 | Feed capable of improving reproductive performance of sow production and preparation method thereof |
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