CN100473723C - 扩增重复核酸序列 - Google Patents

扩增重复核酸序列 Download PDF

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Publication number
CN100473723C
CN100473723C CNB038048671A CN03804867A CN100473723C CN 100473723 C CN100473723 C CN 100473723C CN B038048671 A CNB038048671 A CN B038048671A CN 03804867 A CN03804867 A CN 03804867A CN 100473723 C CN100473723 C CN 100473723C
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China
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primer
nucleic acid
target nucleic
nucleotide
primers
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Expired - Fee Related
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CNB038048671A
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Chinese (zh)
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CN1639352A (zh
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理查德·考索恩
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University of Utah
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University of Utah
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CNB038048671A 2002-01-31 2003-01-31 扩增重复核酸序列 Expired - Fee Related CN100473723C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35359102P 2002-01-31 2002-01-31
US60/353,591 2002-01-31

Publications (2)

Publication Number Publication Date
CN1639352A CN1639352A (zh) 2005-07-13
CN100473723C true CN100473723C (zh) 2009-04-01

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ID=27663226

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CNB038048671A Expired - Fee Related CN100473723C (zh) 2002-01-31 2003-01-31 扩增重复核酸序列

Country Status (9)

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US (2) US7695904B2 (https=)
EP (1) EP1476561B1 (https=)
JP (1) JP4515767B2 (https=)
CN (1) CN100473723C (https=)
AT (1) ATE534746T1 (https=)
AU (1) AU2003208902B2 (https=)
CA (1) CA2474468C (https=)
ES (1) ES2379375T3 (https=)
WO (1) WO2003064615A2 (https=)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3351919A1 (en) 2003-01-24 2018-07-25 University of Utah Methods of predicting mortality risk by determining telomere length
US8080645B2 (en) 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
US8097419B2 (en) 2006-09-12 2012-01-17 Longhorn Vaccines & Diagnostics Llc Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009
US8084443B2 (en) 2007-10-01 2011-12-27 Longhorn Vaccines & Diagnostics Llc Biological specimen collection and transport system and methods of use
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
US8652782B2 (en) * 2006-09-12 2014-02-18 Longhorn Vaccines & Diagnostics, Llc Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids
US9683256B2 (en) 2007-10-01 2017-06-20 Longhorn Vaccines And Diagnostics, Llc Biological specimen collection and transport system
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US10004799B2 (en) 2007-08-27 2018-06-26 Longhorn Vaccines And Diagnostics, Llc Composite antigenic sequences and vaccines
EP2772267B1 (en) 2007-08-27 2016-04-27 Longhorn Vaccines and Diagnostics, LLC Immunogenic compositions and methods
US11041216B2 (en) 2007-10-01 2021-06-22 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
CN101230403B (zh) * 2008-02-21 2010-12-29 浙江理工大学 解析染色体端粒g-末端序列的方法
EP3029154B1 (en) 2008-10-17 2017-10-04 Geron Corporation Method for identification of sensitivity of a patient to telomerase inhibition therapy
CN102439171B (zh) 2008-12-22 2017-07-14 犹他大学研究基金会 单色多重定量pcr
US20110207128A1 (en) * 2010-02-16 2011-08-25 Cawthon Richard M Methods and kits for determining biological age and longevity based on gene expression profiles
JP2011223940A (ja) * 2010-04-21 2011-11-10 Asahi Breweries Ltd 偽陰性を排除するｐｃｒ検査方法およびそれに使用するプライマー
ES2656958T3 (es) * 2011-03-28 2018-03-01 Geron Corporation Medición de la longitud de telómeros en muestras fijadas en formalina, embebidas en parafina (FFPE) por PCR cuantitativa
JP5288387B1 (ja) * 2011-10-13 2013-09-11 学校法人東京女子医科大学 テロメア配列増幅用プライマー
US20130171630A1 (en) * 2011-12-29 2013-07-04 QIAGEN Inc. Methods of using telomeres as markers for aging
EP3494989B1 (en) 2012-01-26 2025-07-16 Longhorn Vaccines and Diagnostics, LLC Composite antigenic sequences and vaccines
WO2013166466A1 (en) * 2012-05-04 2013-11-07 Bio-Rad Laboratories, Inc. Hot-start digital pcr
US9200327B2 (en) 2012-11-30 2015-12-01 Geron Corporation Diagnostic markers for treating cell proliferative disorders with telomerase inhibitors
JP6359028B2 (ja) 2012-11-30 2018-07-18 ジェロン・コーポレーションＧｅｒｏｎ Ｃｏｒｐｏｒａｔｉｏｎ テロメラーゼ阻害剤を用いて細胞増殖性障害を処置するための診断マーカー
US20140287946A1 (en) * 2013-03-14 2014-09-25 Ibis Biosciences, Inc. Nucleic acid control panels
JP2016512696A (ja) * 2013-03-15 2016-05-09 アーノルド, ライル, ジェイ.ARNOLD, Lyle, J. アセンブラ配列を利用する断片化された標的核酸の増幅方法
WO2014152676A1 (en) * 2013-03-15 2014-09-25 New York University A method for a single cell analysis of telomer length
CA2912216A1 (en) 2013-05-22 2014-11-27 Telomere Diagnostics, Inc. Measures of short telomere abundance
GB201418144D0 (en) * 2014-10-14 2014-11-26 Univ Cardiff High throughput sequencing
WO2016108954A1 (en) 2014-12-30 2016-07-07 Telomere Diagnostics, Inc. Multiplex quantitative pcr
EP3294448A4 (en) 2015-05-14 2018-12-12 Longhorn Vaccines and Diagnostics, LLC Rapid methods for the extraction of nucleic acids from biological samples
US10577643B2 (en) * 2015-10-07 2020-03-03 Illumina, Inc. Off-target capture reduction in sequencing techniques
US10186789B1 (en) 2018-04-13 2019-01-22 Rustcraft Industries LLC Keyed cable and connector system
US11639521B2 (en) 2019-06-03 2023-05-02 The Chinese University Of Hong Kong Method for determining the copy number of a tandem repeat sequence
US12485166B2 (en) 2020-02-06 2025-12-02 Longhorn Vaccines And Diagnostics, Llc Vaccines for the treatment and prevention of zoonotic infections
IE20210235A1 (en) 2020-10-20 2023-08-16 Longhorn Vaccines & Diagnostics Llc Immunogenic antigens
CN113862339B (zh) * 2021-12-01 2022-03-22 广州滴纳生物科技有限公司 核酸组合产品、检测试剂盒和扩增靶核酸的方法
CN116218893B (zh) * 2023-03-24 2025-07-29 哈尔滨医科大学 一种通过体外扩增重组质粒在其编码基因上生成短片段串联重复序列的方法
CN117230169B (zh) * 2023-11-13 2024-02-13 元码基因科技（北京）股份有限公司 用于长片段端粒序列测序的接头、预文库及其构建方法
CN117248003B (zh) * 2023-11-13 2024-04-12 元码基因科技（北京）股份有限公司 用于完整端粒扩增子测序的组合物、预文库及其构建方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6020124A (en) * 1992-04-27 2000-02-01 Trustees Of Dartmouth College Detection of soluble gene sequences in biological fluids

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5489508A (en) * 1992-05-13 1996-02-06 University Of Texas System Board Of Regents Therapy and diagnosis of conditions related to telomere length and/or telomerase activity
US5512441A (en) * 1994-11-15 1996-04-30 American Health Foundation Quantative method for early detection of mutant alleles and diagnostic kits for carrying out the method
US5741677A (en) * 1995-06-07 1998-04-21 Geron Corporation Methods for measuring telomere length
JP3718892B2 (ja) * 1996-02-05 2005-11-24 三菱化学株式会社 ヒト・テロメラーゼ活性の測定方法
JP4013333B2 (ja) 1998-06-05 2007-11-28 株式会社Ｂ＆Ｃラボラトリーズ 皮膚の老化度の測定方法および測定用キット
US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6020124A (en) * 1992-04-27 2000-02-01 Trustees Of Dartmouth College Detection of soluble gene sequences in biological fluids

Also Published As

Publication number Publication date
WO2003064615A3 (en) 2004-02-26
EP1476561A4 (en) 2006-08-02
ES2379375T3 (es) 2012-04-25
CA2474468A1 (en) 2003-08-07
US20100151477A1 (en) 2010-06-17
HK1072275A1 (en) 2005-08-19
JP2005515778A (ja) 2005-06-02
ATE534746T1 (de) 2011-12-15
AU2003208902B2 (en) 2008-08-07
EP1476561A2 (en) 2004-11-17
EP1476561B1 (en) 2011-11-23
US8048631B2 (en) 2011-11-01
JP4515767B2 (ja) 2010-08-04
CA2474468C (en) 2014-03-25
CN1639352A (zh) 2005-07-13
US7695904B2 (en) 2010-04-13
WO2003064615A2 (en) 2003-08-07
US20030162266A1 (en) 2003-08-28

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