CN100451647C - Canopy leaf chamber for determining plant canopy population photosynthesis - Google Patents
Canopy leaf chamber for determining plant canopy population photosynthesis Download PDFInfo
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- CN100451647C CN100451647C CNB2006100240918A CN200610024091A CN100451647C CN 100451647 C CN100451647 C CN 100451647C CN B2006100240918 A CNB2006100240918 A CN B2006100240918A CN 200610024091 A CN200610024091 A CN 200610024091A CN 100451647 C CN100451647 C CN 100451647C
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Abstract
The present invention relates to a canopy leaf chamber for measuring the group photosynthesis of a plant canopy, which is formed by combining a square photosynthetic chamber and a cylindrical soil fixing body, wherein a handle is arranged on the soil fixing body at the lower part of the canopy leaf chamber, the base of the canopy leaf chamber is a corrugated cut, and the soil fixing body can be easily inserted into soil to form the closed canopy leaf chamber; an upper opening in the soil fixing body is fitted with a circular opening in the bottom wall of the photosynthetic chamber, and thus, the inner cavity of the canopy leaf chamber forms a whole; the top wall of the photosynthetic chamber is sealed with two-faced adhesive and photosynthetic films to ensure the stable light permeability of the photosynthetic chamber; a small-sized fan is arranged in the side wall of the photosynthetic chamber and controlled by a battery and a switch which are arranged on the outside of the side wall of the photosynthetic chamber, and the fan can rapidly balance the gas in the canopy leaf chamber; plastic hoses are respectively arranged at the center of other two opposite side walls of the photosynthetic chamber and connected with an air passage of a sample room of a photosynthesis measuring device. The present invention can be used for measuring the group photosynthesis, the breathing functions and the transpiration of various plants by combining with the photosynthesis measuring device.
Description
Technical field
The present invention relates to a kind of canopy leaf chamber that is used to measure the effect of plant canopy population photosynthesis, be connected on the sample chamber gas circuit of photosynthesis measurement instrument, according to CO in the canopy leaf chamber
2, O
2, H
2The reference of O changes population photosynthesis, breathing and the transpiration of measuring the canopy leaf chamber implants, belongs to the biological study instrument field.
Background technology
Photosynthesis is that plant utilization luminous energy synthesizes human available organism with inorganicss such as carbon dioxide and water and emits the process of oxygen, and human all food derives from photosynthesis nearly all directly or indirectly.The research of photosynthesis of plant also is the emphasis in the life science always.
The photosynthesis measurement instrument is one of most widely used instrument of research photosynthesis, the U.S., Britain, developed countries such as Germany have dropped into many funds in the research and development of photosynthesis measurement instrument, developed LI-COR series (U.S. LI-COR company respectively, representing instrument is the portable photosynthetic apparatus of LI-COR6400), PPS series (Britain PP Systems scientific instrument company, representing instrument is the portable full-automatic photosynthesis measurement of CIRAS-2 system), HCM series (German WALZ company, representing instrument is the portable photosynthesis analyzer of HCM-1000), these serial instruments all have application more widely in each university of China and related scientific research mechanism.According to incompletely statistics, the present all kinds of photosynthesis measurement instrument of China surpass more than 2000, though production firm's difference of these instruments, its measuring principle all is consistent with the instrument essential structure, all is by CO in the sample leaf chamber
2, O
2, H
2The reference of gases such as O changes measures and calculates photosynthesis, respiration, transpiration, and therefore, sample leaf chamber is very important in the photosynthesis measurement instrument.
The leaf chamber of present most of photosynthesis measurement instrument is all designed according to broad leaved plant, and majority is designed to 2 * 3cm
2About rectangle leaf chamber (as: LI-COR 6400), need sandwich the leaf chamber to the blade that will measure during measurement and measure.Yet, because the kind difference of plant, it is very big that the blade shape of plant differs, single leaf chamber design is difficult to satisfy the mensuration requirement to different floristics blades, therefore, Britain PPSystems scientific instrument company has developed wide leaf chamber at different plant leaf blades, rice leaf chamber, the narrow leaf chamber, the needle chamber, beanpod leaf chamber, liver moss leaf chamber, are are leaf chambers such as ball-type leaf chamber configured on the photosynthetic instrument of CIRAS-2, and different plant leaf blades carried out photosynthetic mensuration (the http://www.qudao.com.cn/article.asp of Beijing channel science equipment company limited? articleid=221).But, because the blade shape of many plants is all irregular, particularly the blade as the grasses of main crops all is bar shaped, lanceolar or aciculiform blade, single leaf can not be evenly distributed in the leaf chamber when measuring and measure, and the measuring error of the overlapping and leaf area of blade can cause the failure of its photosynthesis measurement in the leaf chamber.In addition, the photosynthesis of plant representativeness of single blade is very limited, be difficult to the accurately photosynthetic capacity and the assimilation output of reflection plant reality, and the population photosynthesis of plant could be represented the optical energy utilization efficiency of plant better, the actual photosynthesis output that more can reflect plant, so the research of population photosynthesis effect becomes focus gradually.Because photosynthetic, breathing and the transpiration of plant need be based on CO in the sample leaf chamber
2, O
2, H
2The reference of O concentration changes to be measured and calculates, so following condition must be satisfied in the canopy chamber of research population photosynthesis: 1) positive confinement guarantees CO in the leaf chamber
2, O
2, H
2The variation of gas concentrations such as O reflects photosynthetic, breathing and the transpiration of plant truly; 2) gas equilibrium is fast, guarantees the CO in the leaf chamber
2, O
2, H
2Gases such as O reach balance in very short time, and are evenly distributed; 3) light transmission is good, guarantees normal illumination condition of plant population and normal photosynthetic capacity; 4) finish once mensuration within a short period of time, to avoid sealing the interference that the canopy indoor temperature raises.Yet up to now, numerous photosynthetic instrument companies all do not develop fairly perfect mensuration plant population photosynthetic canopy chamber.
In order to measure the population photosynthesis of plant, PP Systems scientific instrument company adopts the cylindric beaker back-off of transparent polyethylene to come to have made easy canopy leaf chamber, canopy leaf chamber top and be transparent vinyon integral body all around, fixing in the bottom of its opening with stainless steel garden ring, and connect gas circuit and link to each other with gas analyzing apparatus.The canopy leaf chamber that is used for lawn population photosynthesis mensuration is CPY-2, and its end directly is 15cm, and height is 14cm, canopy leaf chamber is covered on the lawn be used for measuring the population photosynthesis effect that covers on lawn plant in the canopy leaf chamber on the ground; The canopy leaf chamber that is used for potted plant is CPY-3, its end directly is 13cm, height is similarly 14cm, canopy leaf chamber is covered on the population photosynthesis effect (http://www.ppsystems.com/chambers_1.html#CPY_OPEN) of measuring potted plant on the basin limit mouth of planting plant.There are following three big defectives in this easy canopy leaf chamber:
1, the sealing of canopy leaf chamber is relatively poor, there is the space inevitably between special canopy leaf chamber open base and lawn plant (CPY-2) or the basin limit mouth (CPY-3), no matter firmly compress or mould wrapping with other glue, gas in the canopy leaf chamber always has a certain amount of exchange by the space and the external world, and this insecure sealing brings great error can for the photosynthetic mensuration of canopy;
2, owing to will touch silt inevitably in the photosynthetic mensuration process of canopy, after measuring several times, the polythene material of canopy chamber can be worn, the light transmission variation, thereby influence indoor light intensity of canopy and light quality, cause the error of photosynthesis measurement, cause on same level, to compare between the measured value of different batches.
3, turnover gas circuit susceptible to.The gas circuit of CPY-2 and CPY-3 is imported and exported the base portion of design at canopy leaf chamber, the below of photosynthetic organs such as the leaf of plant, stem when being positioned at mensuration, the gas exchange of photosynthesis generation is diffused into gas circuit and will be subjected to stopping of cauline leaf on the one hand, seriously is subjected to the interference of canopy leaf chamber base portion sealing difference on the other hand.
Summary of the invention
The objective of the invention is at existing photosynthesis of plant analyzer in the deficiency aspect the effect of mensuration plant canopy population photosynthesis, a kind of canopy leaf chamber that is used to measure the effect of plant canopy population photosynthesis is provided, good airproof performance, light transmission is strong, can make things convenient for photosynthetic, breathing and the transpiration of measuring the canopy leaf chamber implants exactly.
For realizing this purpose, the canopy leaf chamber that the present invention's design provides is mainly by foursquare photosynthetic chamber and columned soil fixing body two parts be combined into.The soil fixing body that is positioned at the canopy leaf chamber bottom is provided with handle, base portion is the corrugated otch, hand-held handle can insert fixed body at an easy rate and form airtight canopy leaf chamber in the soil, the fixed body circular open with position photosynthetic chamber diapire thereon suitable for reading fits like a glove, and makes the inner chamber of canopy leaf chamber form an integral body.The roof of photosynthetic chamber is with double faced adhesive tape and the photosynthetic diaphragm seal that can often change, with the light transmission that guarantees that photosynthetic chamber is stable.A small-sized fan is installed in a sidewall of photosynthetic chamber, and fan is by being installed in outer battery of sidewall and switch control.In addition two relative side wall centers are respectively installed a plastic flexible pipe and are connected with the sample chamber gas circuit of photosynthesis measurement instrument in photosynthetic chamber, can detect CO in the canopy leaf chamber by photosynthetic apparatus
2, O
2, H
2The variation of gases such as O, and change population photosynthesis effect, respiration and the transpiration of measuring the canopy leaf chamber implants according to its reference.
The concrete structure that is used to measure the canopy leaf chamber of plant canopy population photosynthesis effect of the present invention is: canopy leaf chamber is made up of foursquare photosynthetic chamber and columned soil fixing body two parts.Sidewall and diapire are made by transparent organic glass around the photosynthetic chamber, to guarantee the light transmission of canopy leaf chamber.A small-sized fan is installed in a sidewall of photosynthetic chamber, and small fan can make photosynthetic indoor gas reach equilibrium state rapidly by being installed in outer battery of sidewall and switch control when opening fan.The roof of photosynthetic chamber double faced adhesive tape and photosynthetic diaphragm seal, photosynthetic film can often be changed, to guarantee photosynthetic chamber good light transmittance.The relative side wall centers in two sides in addition of photosynthetic chamber is installed a pair of plastic flexible pipe respectively, and photosynthetic indoor gas communicates with the sample chamber gas circuit of photosynthesis measurement instrument by plastic flexible pipe.
The diapire of photosynthetic chamber is connected with columned soil fixing body, the soil fixing body can adopt pmma material, also can adopt transparent hard plastic materials such as PVC, the diapire circular open of the suitable for reading and photosynthetic chamber of soil fixing body is intact close identical, to guarantee the unobstructed and complete of gas in the canopy leaf chamber, a pair of handle is set on the two side of soil fixing body, and the base portion opening part of soil fixing body is made sharp corrugated otch.Hold handle, can at an easy rate soil be inserted in the lower end of soil fixing body, utilize the sealing property of soil to avoid the gas leakage of canopy leaf chamber base portion.
The present invention is provided with plastic flexible pipe and connects the photosynthesis measurement instrument in the both sides of the photosynthetic chamber of canopy leaf chamber, photosynthetic chamber promptly becomes the sample chamber of photosynthesis measurement instrument.Pinning handle rotates gently, the soil fixing body of canopy leaf chamber inserted to plant remain in the soil or Culture basin of measuring plants, open fan then, make the gas in the canopy leaf chamber reach equilibrium state rapidly, detect gas situation of change in the canopy leaf chamber with photosynthetic instrument, can measure the indexs such as population photosynthesis effect, respiration, transpiration of plant behind the ready to balance.
Canopy leaf chamber of the present invention can be connected with existing photosynthesis measurement instrument, be used to measure population photosynthesis, breathing and the transpiration of each kind of plant such as lawn, meadow, flowers, cover plant, vegetables, cereal crops, potted plant seedling, undershrub, cover the respiration that also can be used for measuring soil microorganism behind the gobo.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Among Fig. 1,1 is photosynthetic chamber, and 2 is fan battery and switch, and 3 is fan, and 4 is the circular open of photosynthetic chamber diapire, and 5 is plastic flexible pipe, and 6 is handle, and 7 are the soil fixing body, and 8 are soil fixing body corrugated otch, and 9 is photosynthetic film.
Embodiment
Below in conjunction with accompanying drawing technical scheme of the present invention is further described.
Canopy leaf chamber structure of the present invention is made up of foursquare photosynthetic chamber 1 and columned soil fixing body 7 two parts as shown in Figure 1.Sidewall and diapire are made by transparent organic glass around the photosynthetic chamber 1, to guarantee the light transmission of canopy leaf chamber.A small-sized fan 3 (can adopt the small fan that dispels the heat for CPU on the household PC) is installed in a sidewall of photosynthetic chamber 1, fan 3 is by being installed in 2 outer joint No.5 cells of sidewall and switch 2 controls, the roof of photosynthetic chamber 1 seals with double faced adhesive tape and photosynthetic film 9, photosynthetic film can often be changed, to guarantee photosynthetic chamber 1 good light transmittance, the relative side wall centers in two sides in addition of photosynthetic chamber is respectively installed a plastic flexible pipe 5, and the gas in the photosynthetic chamber 1 communicates with the sample chamber gas circuit of photosynthesis measurement instrument by plastic flexible pipe 5.
The diapire of photosynthetic chamber 1 is connected with columned soil fixing body 7, soil fixing body 7 can adopt pmma material, also can adopt transparent hard plastic materials such as PVC, the circular open 4 of chamber suitable for reading and photosynthetic 1 diapire of soil fixing body 7 is intact close identical, to guarantee the unobstructed and complete of gas in the canopy leaf chamber, a pair of handle 6 is set on the two side of soil fixing body 7, and the base portion opening part of soil fixing body 7 is made sharp corrugated otch 8.
During use, untie photosynthesis measurement instrument main frame and measure a screw that the sample chamber gas circuit links to each other, link to each other with pairing screw on the plastic flexible pipe 5 of two side, the photosynthetic chamber of canopy leaf chamber respectively, photosynthetic chamber promptly becomes the sample chamber of photosynthesis measurement instrument, and the photosynthesis measurement instrument can be according to the CO in the canopy leaf chamber
2, O
2, H
2The reference of O changes photosynthetic, breathing and the transpiration of measuring the canopy leaf chamber implants.
During mensuration, both hands are pinned handle 7, rotation gently, can insert the soil fixing body 7 of canopy leaf chamber to plant and remain in the soil or Culture basin of measuring plants, general lawn ground soil fixing body insertion depth reaches 5-8cm and gets final product, other plant can make suitably to adjust (2-10cm) according to the elasticity of the height of plant and soil, utilizes the sealing property of soil to avoid the defective of canopy leaf chamber base portion gas leakage.Insert good after, open fan, make the gas in the canopy leaf chamber reach equilibrium state rapidly, detect the interior gas situation of change of canopy leaf chamber with photosynthetic instrument, can measure the indexs such as population photosynthesis effect, respiration, transpiration of plant behind the ready to balance.
Canopy leaf chamber of the present invention can be made 15cm (diameter and the length of side) * 20cm (height, wherein: photosynthetic chamber height is 12cm, and fixedly height is 8cm).The size of canopy leaf chamber can be adjusted according to the different works of measuring vegetable material and cultivating container are corresponding, and the diameter and the length of side can be made 5-50cm, and height can be made 12-200cm.
Canopy leaf chamber of the present invention is with the photosynthesis measurement instrument, can be used for measuring population photosynthesis, breathing and the transpiration of each kind of plant such as lawn, meadow, flowers, cover plant, vegetables, cereal crops, potted plant seedling, undershrub, cover the respiration that also can be used for measuring soil microorganism behind the gobo.
Claims (2)
1, a kind of canopy leaf chamber that is used to measure the effect of plant canopy population photosynthesis, it is characterized in that forming by foursquare photosynthetic chamber (1) and columned soil fixing body (7) two parts, sidewall and diapire are made by transparent organic glass around the photosynthetic chamber (1), fan (3) is installed in one sidewall of photosynthetic chamber (1), by being installed in outer battery of sidewall and switch (2) control, the roof of photosynthetic chamber (1) seals with double faced adhesive tape and photosynthetic film (9), the relative side wall centers in two sides in addition of photosynthetic chamber (1) is respectively installed a plastic flexible pipe (5), and the gas in photosynthetic chamber (1) communicates with the sample chamber gas circuit of photosynthesis measurement instrument by plastic flexible pipe (5); The diapire of photosynthetic chamber (1) is connected with columned soil fixing body (7), the circular open (4) of chamber suitable for reading and photosynthetic (1) diapire of soil fixing body (7) is intact close identical, a pair of handle (6) is set on the two side of soil fixing body (7), and the base portion opening part of soil fixing body (7) is made sharp corrugated otch (8); Described soil fixing body (7) is made by organic glass or transparent PVC duroplasts.
2, according to the canopy leaf chamber that is used to measure the effect of plant canopy population photosynthesis of claim 1, it is characterized in that the length of side of described photosynthetic chamber (1) is identical with the diameter of soil fixing body (7), be 5-50cm, whole canopy leaf chamber height is 12-200cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100240918A CN100451647C (en) | 2006-02-23 | 2006-02-23 | Canopy leaf chamber for determining plant canopy population photosynthesis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100240918A CN100451647C (en) | 2006-02-23 | 2006-02-23 | Canopy leaf chamber for determining plant canopy population photosynthesis |
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| CN1815226A CN1815226A (en) | 2006-08-09 |
| CN100451647C true CN100451647C (en) | 2009-01-14 |
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Cited By (1)
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| CN103954562A (en) * | 2014-04-09 | 2014-07-30 | 福建农林大学 | Photosynthesis machine based on optical fiber sensor |
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| CN112345695B (en) * | 2020-10-27 | 2022-12-09 | 湖南农业大学 | Method for measuring photosynthesis of plant population |
| CN113552286B (en) * | 2021-06-08 | 2023-03-28 | 广东省农业科学院植物保护研究所 | Method and instrument for measuring plant stem photosynthesis rate |
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