CN100447242C - 纯化的乳糖酶 - Google Patents

纯化的乳糖酶 Download PDF

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Publication number
CN100447242C
CN100447242C CNB028072863A CN02807286A CN100447242C CN 100447242 C CN100447242 C CN 100447242C CN B028072863 A CNB028072863 A CN B028072863A CN 02807286 A CN02807286 A CN 02807286A CN 100447242 C CN100447242 C CN 100447242C
Authority
CN
China
Prior art keywords
lactase
lactase soln
milk
soln
oligosaccharides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
CNB028072863A
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English (en)
Chinese (zh)
Other versions
CN1500143A (zh
Inventor
R·F·W·C·范比克霍文
P·A·范帕里顿
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8176033&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN100447242(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of CN1500143A publication Critical patent/CN1500143A/zh
Application granted granted Critical
Publication of CN100447242C publication Critical patent/CN100447242C/zh
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
    • A23C9/1206—Lactose hydrolysing enzymes, e.g. lactase, beta-galactosidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C2210/00—Physical treatment of dairy products
    • A23C2210/20—Treatment using membranes, including sterile filtration
    • A23C2210/208—Removal of bacteria by membrane filtration; Sterile filtration of milk products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01108—Lactase (3.2.1.108)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Dairy Products (AREA)
  • Enzymes And Modification Thereof (AREA)
CNB028072863A 2001-04-04 2002-04-03 纯化的乳糖酶 Ceased CN100447242C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01000102 2001-04-04
EP01000102.2 2001-04-04

Publications (2)

Publication Number Publication Date
CN1500143A CN1500143A (zh) 2004-05-26
CN100447242C true CN100447242C (zh) 2008-12-31

Family

ID=8176033

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB028072863A Ceased CN100447242C (zh) 2001-04-04 2002-04-03 纯化的乳糖酶

Country Status (8)

Country Link
US (3) US7955831B2 (https=)
EP (2) EP1373491B1 (https=)
JP (6) JP2004534527A (https=)
CN (1) CN100447242C (https=)
DK (2) DK2280065T3 (https=)
ES (2) ES2564821T3 (https=)
PT (2) PT2280065T (https=)
WO (1) WO2002081673A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107858342A (zh) * 2017-10-11 2018-03-30 华中农业大学 一种稳定的乳糖酶液态制剂

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1373491B1 (en) * 2001-04-04 2015-12-30 DSM IP Assets B.V. Purified lactase
AU2013200779C1 (en) * 2005-11-28 2022-11-10 Dsm Ip Assets B.V. Enzyme preparations yielding a clean taste
ES2454167T3 (es) 2005-11-28 2014-04-09 Dsm Ip Assets B.V. Preparación de enzima que produce un sabor puro
US8236297B2 (en) * 2007-10-11 2012-08-07 Kenneth Manzo Method of treating lactose intolerance using genetically engineered bacteria
ES2591359T3 (es) 2007-12-03 2016-11-28 Novozymes A/S Método para producir un producto lácteo de bajo contenido en lactosa
EP2272374B1 (en) 2009-06-23 2015-01-14 Nestec S.A. Lactase containing milk powder
US20140087404A1 (en) * 2011-03-14 2014-03-27 Godo Shusei Co., Ltd. Method for assaying arylsulfatase activity
WO2014185364A1 (ja) 2013-05-13 2014-11-20 合同酒精株式会社 ラクターゼ含有組成物の製造法
US10201178B2 (en) * 2014-03-05 2019-02-12 Dsm Ip Assets B.V. Stable liquid lactase compositions
EP3187582B1 (en) * 2014-08-27 2025-07-23 Godo Shusei Co., Ltd. Lactase solution
WO2016060224A1 (ja) * 2014-10-17 2016-04-21 合同酒精株式会社 ラクターゼ溶液及びそれを用いた乳
BR112017011607A2 (pt) 2014-12-05 2018-01-09 Godo Shusei Kk solução de lactase e produto lácteo usando a mesma
AR107872A1 (es) 2016-03-16 2018-06-13 Godo Sushei Co Ltd Proteinasa b y solución de lactasa usando sus propiedades y método para producirlo
WO2018130654A1 (en) * 2017-01-13 2018-07-19 Novozymes A/S Sterile filtered lactase preparation comprising salt with monovalent cation
US11517613B2 (en) * 2017-06-07 2022-12-06 Amano Enzyme Inc. Lactase bulk powder and lactase preparation
WO2021134002A1 (en) 2019-12-26 2021-07-01 Pharmunion, LLC Stable lactase product
JP7706443B2 (ja) * 2020-04-17 2025-07-11 合同酒精株式会社 容器詰めラクターゼ溶液
US20250049057A1 (en) 2021-09-07 2025-02-13 Godo Shusei Co., Ltd. Lactase, lactase preparation, gene, recombinant vector, and transformant
JPWO2023106420A1 (https=) 2021-12-09 2023-06-15

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237230A (en) * 1977-05-31 1980-12-02 Daiwa Kasei Kabushiki Kaisha Novel lactase
EP0014502B1 (en) * 1979-02-06 1983-07-27 THE PROCTER & GAMBLE COMPANY Soap bars

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1306751A (en) * 1971-01-26 1973-02-14 Baxter Laboratories Inc Lactase enzyme production
US3753725A (en) 1971-03-11 1973-08-21 R Williams Method for enzymatic conversion of lactose to glucose and galactose in lactose containing fluids
US3919049A (en) 1974-06-27 1975-11-11 Tokyo Tanabe Co Process for preparing {62 -galactosidase
SE438773B (sv) 1974-11-13 1985-05-13 Tetra Pak Int Sett att framstella en steril mejeriprodukt med nedsatt halt av laktos
US4007283A (en) * 1975-10-28 1977-02-08 The Regents Of The University Of California Production and utilization of lactase
JPS5571445A (en) 1978-11-24 1980-05-29 Morinaga Milk Ind Co Ltd Production of lactose hydrolyzate
JPS5651984A (en) 1979-10-02 1981-05-09 Sumitomo Chem Co Ltd Fixed lactase and its preparation
JPS56140890A (en) 1980-04-04 1981-11-04 Sumitomo Chem Co Ltd Immobilized lactase and its preparation
FR2500269A1 (fr) 1981-02-20 1982-08-27 Tetra Pak Int Procede pour le traitement des produits laitiers pour diminuer leur teneur en lactose notamment
JPS59175879A (ja) 1983-03-25 1984-10-04 Snow Brand Milk Prod Co Ltd 酵素における汚染細菌の殺菌法
AU586809B2 (en) 1983-12-02 1989-07-27 Gist-Brocades N.V. Process for the preparation of a germ free solution of a lactose-splitting enzyme and its use.
US5283189A (en) 1987-02-09 1994-02-01 Research Development Corporation Of Japan β-galactosidase from Thermus sp
US5357852A (en) 1992-06-02 1994-10-25 Kohler Mix Specialties Method and apparatus for producing reduced lactose milk
US5744345A (en) * 1992-10-05 1998-04-28 Takara Shuzo Co., Ltd. Hyperthermostable β-galactosidase gene, enzyme encoded thereby, and process for production
US5821350A (en) 1995-11-01 1998-10-13 Nexia Biotechnologies, Inc. Aspergillus niger beta-galactosidase gene
ES2130991B1 (es) 1997-05-16 2000-04-01 Plumley Kevin More Procedimiento que implica la pre-digestion parcial en una linea de produccion para la elaboracion de leche y derivados lacteos libres de lactosa para evitar descargas de 5-hidroxi-triptamina - o 5-ht.
US6146902A (en) 1998-12-29 2000-11-14 Aventis Pasteur, Inc. Purification of polysaccharide-protein conjugate vaccines by ultrafiltration with ammonium sulfate solutions
EP1261697B1 (en) * 1999-07-09 2004-07-28 Université de Liège Cold-active beta galactosidase, the process for its preparation and the use thereof
EP1373491B1 (en) 2001-04-04 2015-12-30 DSM IP Assets B.V. Purified lactase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237230A (en) * 1977-05-31 1980-12-02 Daiwa Kasei Kabushiki Kaisha Novel lactase
EP0014502B1 (en) * 1979-02-06 1983-07-27 THE PROCTER & GAMBLE COMPANY Soap bars

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107858342A (zh) * 2017-10-11 2018-03-30 华中农业大学 一种稳定的乳糖酶液态制剂
CN107858342B (zh) * 2017-10-11 2020-09-25 华中农业大学 一种稳定的乳糖酶液态制剂

Also Published As

Publication number Publication date
US20110212221A1 (en) 2011-09-01
PT2280065T (pt) 2021-02-16
JP2016000058A (ja) 2016-01-07
DK2280065T3 (da) 2021-03-29
JP2004534527A (ja) 2004-11-18
JP2009034116A (ja) 2009-02-19
US9149048B2 (en) 2015-10-06
EP1373491A1 (en) 2004-01-02
DK1373491T3 (en) 2016-03-14
US9763460B2 (en) 2017-09-19
ES2564821T3 (es) 2016-03-29
JP2013099364A (ja) 2013-05-23
EP2280065B1 (en) 2021-01-13
PT1373491E (pt) 2016-03-31
EP2280065A1 (en) 2011-02-02
JP2010148516A (ja) 2010-07-08
US20150359239A1 (en) 2015-12-17
US20040121041A1 (en) 2004-06-24
EP1373491B1 (en) 2015-12-30
WO2002081673A1 (en) 2002-10-17
US7955831B2 (en) 2011-06-07
CN1500143A (zh) 2004-05-26
JP2014050399A (ja) 2014-03-20
ES2858527T3 (es) 2021-09-30

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PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
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GR01 Patent grant
C35 Partial or whole invalidation of patent or utility model
IW01 Full invalidation of patent right

Decision date of declaring invalidation: 20101203

Decision number of declaring invalidation: 15683

Granted publication date: 20081231