CN100439508C - Process for preparing oat beta-glucans - Google Patents
Process for preparing oat beta-glucans Download PDFInfo
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- CN100439508C CN100439508C CNB200610097431XA CN200610097431A CN100439508C CN 100439508 C CN100439508 C CN 100439508C CN B200610097431X A CNB200610097431X A CN B200610097431XA CN 200610097431 A CN200610097431 A CN 200610097431A CN 100439508 C CN100439508 C CN 100439508C
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Abstract
This inventnion relates to a preparation method for high-efficiency prebiotics oat beta-glucan, belonging to the quintessential processing technologies of oat. The said method includes using oat bran as raw materials, crushing, microwave-assisted-extracting, adding amylase and glucoamylase, isoelectric-point-precipitating, centrifugating and separating, concentrating the supernatant, precipitating with ethanol, centrifugating and collecting the precipitation, solving with water, hydrolyzing with beta-glucanase, and spray-drying process to obtain the high-efficiency prebiotics oat beta-glucan with significantly enhanced intestinal and fecal bifidobacteria and actobacillus value-added effect. The method is scientifically and rationally designed, and of great significance in achieving the comprehensive utilization of resources oat and increasing its added value.
Description
Technical field
A kind of preparation method of efficient prebiotics avenabeta glucosan belongs to oat deep process technology field.
Background technology
Oat is a kind of cereal that has nutritive value and nourishing function concurrently.According to statistics, the oat cultivated area of China in 2003 is 361.65 ten thousand mu, 204.6 kilograms of average yield per mus, and ultimate production is 740,000 tons.Along with the pay attention to day by day of people to self diet health, the sustainable growth of global oat consumption, the world market is in great demand to avenaceous.Yet China's oat deep process technology is relatively backward at present, oat based food kind is single on the market, cause 4% of the not enough ultimate production of China's oat human consumption part, this situation has not only caused the significant wastage of resource, and has limited further developing of China's oat industry.
The existing avenabeta glucosan that studies show that has reducing blood-fat, effect such as hypoglycemic as a kind of fine food fibre, but its whether to intestinal function produce positive affact not clear so far.Therefore the present invention is raw material with the oat bran, go out significantly to promote the efficient prebiotics avenabeta glucosan product of enteron aisle and ight soil bifidus bacillus, lactobacillus increment effect by rational prepared, and make an addition in the food as a kind of novel food ingredients, play the effect of prebiotics with prestige, realize the comprehensive utilization of oat resource simultaneously.
Summary of the invention
The preparation method who the purpose of this invention is to provide a kind of efficient prebiotics avenabeta glucosan, products obtained therefrom can significantly promote enteron aisle and ight soil bifidus bacillus, lactobacillus increment effect.
Technical scheme of the present invention: with the oat bran is raw material, through pulverize, microwave-assisted extracts, add amylase and saccharifying enzyme processing, isoelectric precipitation, centrifugation, supernatant concentration, ethanol sedimentation, centrifugation collecting precipitation, obtain significantly promoting enteron aisle and ight soil bifidus bacillus after adding water redissolution, beta-glucan enzymic hydrolysis, spray-drying process, the efficient prebiotics avenabeta glucosan product of lactobacillus increment effect.
(1) pulverizing of raw material
Requirement is pulverized the raw material oat bran, and crosses the 0.5mm screen cloth.The raw material pulverizing effect is good more, helps the extraction of beta-glucan in the oat bran more.
(2) microwave-assisted extracts
Solid-liquid ratio: the mass ratio of oat bran and water is 1: 12, reduces water consumption and can cause the avenabeta glucosan extraction yield on the low side, increases water consumption and can cause producing the load increasing again, and production cost increases.Therefore relatively more suitable solid-liquid ratio is 1: 12.
Microwave power and treatment time: microwave power is the key factor that influences oat bran beta-glucan extraction yield.Increase the increase that microwave power helps extraction yield; But power is also unsuitable excessive, otherwise can cause the increase of energy consumption and the destruction of beta-glucan molecular structure, and therefore the microwave power of selecting is 720W.Same reason, microwave treatment time is unsuitable long or too short, is advisable about 9min.
The pH value: the pH value is less to the influence of beta-glucan extraction yield in the microwave-assisted extraction process, and weakly alkaline environment helps the dissolving of beta-glucan, so be decided to be pH value 9.0.
(3) enzymatic treatment
Thereby the enzymatic treatment extracting solution is the purpose that becomes small molecules to reach to be removed for degraded starch.Under 90-100 ℃ of condition, add heat-resistant alpha-amylase (30U/ml reaction solution) and carry out stir about 30min, till the constant orchid of iodine liquid, reduce the extracting solution temperature subsequently to 55-60 ℃, transfer extracting solution pH to 4.5, add liquid saccharifying enzyme (50U/ml reaction solution), stir 15-30min.
(4) isoelectric precipitation
The purpose of isoelectric precipitation is to remove the avenin that is dissolved in the extracting solution.Therefore the iso-electric point of avenin remains on the pH of extracting solution 4.5 and leaves standstill 30min and can at utmost precipitate avenin about pH4.5.
(5) centrifugation behind the enzyme of going out
Enzyme work remaining in the extracting solution enzyme that goes out is handled, extracting solution is warming up to 100 ℃ keeps 10min.Adopt link-suspended basket centrifuge extracting solution effectively can be separated after reducing to room temperature, centrifugal condition is 3000r/min, 20min, collects supernatant liquor, transfers pH to neutral.
(6) supernatant concentration
With falling film type economic benefits and social benefits thickener supernatant liquor is concentrated, thickening temperature 60-65 ℃, concentrate about one times.
(7) ethanol sedimentation
Certain density ethanol can be settled out the polysaccharide in the solution.Generally speaking, alcohol concn is high more, and the sedimentation effect of polysaccharide is good more, is unfavorable for optionally precipitating beta-glucan again but alcohol concn is too high.For guaranteeing the requirement of deposition rate and purity two aspects, the suitableeest ethanol final concentration is 60%.
(8) centrifugation
Through the ethanol sedimentation process of previous step, most of beta-glucan is precipitated in the solution comes out, and must reclaim beta-glucan by the method for centrifugation, adopts link-suspended basket centrifuge centrifugation (3000r/min, 30min) to reclaim precipitation.
(9) precipitation adds the water redissolution
In precipitation, add water by 1: 10 mass ratio and stir redissolution, prepare to carry out next step enzymolysis process.
(10) beta-glucan enzymic hydrolysis
The suitableeest action condition of beta-glucanase is a pH value 6.5, and 50 ℃ of temperature can obtain desired product in 60 minutes in adding the beta-glucanase effect than the ratio of E/S=1% at the bottom of the enzyme with this understanding.After enzyme digestion reaction finishes, in order to stop the further effect of beta-glucanase, the residual enzyme activity enzyme that goes out is handled, enzymolysis solution is warming up to 100 ℃, and keeps 10min.
(11) spraying drying
Enzymolysis solution is carried out spraying drying, and air outlet temperature is 85 ℃, and moisture content is below 5%, and dried product is efficient prebiotics avenabeta glucosan.
The short intestinal beneficial bacterium growth activity of efficient prebiotics avenabeta glucosan detects
Irritating stomach dosage is 0.25g/kgd, and control group is irritated stomach and give equal in quality physiological saline every day, and each organizes equal food and the drinking-water freely got of mouse, irritates 28 days gastric phases.Avenabeta glucosan is as shown in table 1 to the influence of intestinal bacteria, bifidus bacillus and lactobacillus in stool in mice and the enteron aisle.As can be known from Table 1, bifidus bacillus in the time of 28 days in mouse intestinal and the ight soil and lactobacillus number are than the remarkable increase of control group, and the intestinal bacteria number reduces simultaneously, illustrates that efficient prebiotics avenabeta glucosan can be used as prebiotics.
Table 1 avenabeta glucosan is to the influence of stool in mice and enteron aisle intestinal bacteria, bifidus bacillus and lactobacillus
The enzyme work of used heat-resistant alpha-amylase is 20 in the preparation process of avenabeta glucosan, 000U/mL, and the enzyme work of liquid saccharifying enzyme is 50,000U/mL, the enzyme of beta-glucanase live and are 40U/ml.
Prepared avenabeta glucosan is a white powder, and relative molecular mass is 5000～10000, and enteron aisle and ight soil bifidus bacillus, lactobacillus are had the increment of promotion effect.
Beneficial effect of the present invention: the efficient prebiotics avenabeta glucosan that adopts the inventive method preparation, can play the growth that promotes probiotics (bifidus bacillus and lactobacillus) in enteron aisle and the ight soil effectively, also can suppress colibacillary breeding simultaneously, therefore can be used as the immunizing power that prebiotics is applied to be used in the food improving body.
Description of drawings
The efficient prebiotics avenabeta glucosan of Fig. 1 preparation technology schematic flow sheet.
Embodiment
Embodiment 1
The pulverizing of learning from else's experience, cross the 10kg oat bran of 0.5mm screen cloth, add entry 120L stirring and evenly mixing, regulate pH value to 9.0, after handling 9min under the condition of microwave power 720W, be warming up to 95 ℃, stir about 30min behind the adding heat-resistant alpha-amylase 0.18L is to the constant orchid of iodine liquid, reduce extracting solution temperature to 55 ℃, transfer extracting solution pH4.5, add liquid saccharifying enzyme 0.12L, continue stirring reaction 30min, staticly settle 30min, enzymolysis is finished post-heating to 100 ℃ insulation 10min, be cooled to the room temperature centrifugation (3000r/min, 20min), collecting supernatant liquor transfers pH to neutral, concentrate one times with falling film type economic benefits and social benefits thickener, (3000r/min 30min) reclaims precipitation behind the adding dehydrated alcohol 55L suspension liquid to be carried out centrifugation, in precipitation, add 8L water stirring and dissolving, add 8g beta-glucanase effect 60 minutes behind the adjust pH 6.5,50 ℃ of temperature, after the end enzymolysis solution is warming up to 100 ℃, and keeping 10min, cooling back spraying drying obtains efficient prebiotics avenabeta glucosan product 0.8kg.
Embodiment 2
The pulverizing of learning from else's experience, cross the 15kg oat bran of 0.5mm sieve, add entry 180L stirring and evenly mixing, regulate pH value to 9.0, after handling 9min under the condition of microwave power 720W, be warming up to 90 ℃, stir about 25min behind the adding heat-resistant alpha-amylase 0.27L is to the constant orchid of iodine liquid, reduce extracting solution temperature to 60 ℃, transfer extracting solution pH4.5, add liquid saccharifying enzyme 0.18L, continue stirring reaction 15min, staticly settle 30min, enzymolysis is finished post-heating to 100 ℃ insulation 10min, be cooled to the room temperature centrifugation (3000r/min, 20min), collecting supernatant liquor transfers pH to neutral, concentrate one times with falling film type economic benefits and social benefits thickener, (3000r/min 30min) reclaims precipitation behind the adding dehydrated alcohol 90L suspension liquid to be carried out centrifugation, in precipitation, add 12L water stirring and dissolving, add 12g beta-glucanase effect 60 minutes behind the adjust pH 6.5,50 ℃ of temperature, after the end enzymolysis solution is warming up to 100 ℃, and keeping 10min, cooling back spraying drying obtains efficient prebiotics avenabeta glucosan product 1.2kg.
Claims (3)
1, a kind of preparation method of avenabeta glucosan, it is characterized in that with the oat bran being raw material, through pulverize, microwave-assisted extracts, add amylase and saccharifying enzyme processing, isoelectric precipitation, centrifugation, supernatant concentration, ethanol sedimentation centrifugation collecting precipitation, add the water redissolution, beta-glucan enzymic hydrolysis, spraying drying obtain the avenabeta glucosan product, technology is:
(1) raw material pulverizing: the raw material oat bran is pulverized, and crosses the 0.5mm screen cloth;
(2) microwave-assisted extracts: the oat bran after the pulverizing adds water by 1: 12 mass ratio, regulates pH value to 9.0, handles 9 minutes under the condition of microwave power 720W;
(3) enzymatic treatment: extracting solution adds heat-resistant alpha-amylase and stirs under 90-100 ℃ of condition, every milliliter of extracting solution adds the 30U heat-resistant alpha-amylase, till the constant basket of iodine liquid, reduce temperature to 55-60 ℃, regulate extracting solution pH to 4.5, add the liquid saccharifying enzyme, every milliliter of extracting solution adds the 50U saccharifying enzyme, stirs 15-30 minute;
(4) isoelectric precipitation: keep pH value 4.5, left standstill 30 minutes;
(5) centrifugation behind the enzyme of going out: extracting solution is heated to 100 ℃, is incubated 10 minutes, transfer pH to neutral after supernatant liquor is collected in 3000 rev/mins of centrifugations 20 minutes;
(6) supernatant concentration: concentrated condition is temperature 60-65 ℃, concentrates one times;
(7) ethanol sedimentation: in concentrated solution, add ethanol, make that the ethanol final concentration reaches 60% in the solution;
(8) centrifugation: with the suspension liquid behind the ethanol sedimentation through 3000 rev/mins centrifugal 30 minutes, collecting precipitation;
(9) adding water redissolves: add the water stirring and dissolving by 1: 10 mass ratio in precipitation;
(10) beta-glucan enzymic hydrolysis:, after 50 ℃ of the temperature, add beta-glucanase effect 60 minutes in the ratio of enzyme concentration and substrate ratio E/S=0.01 with solution adjust pH to 6.5; Reaction is warming up to 100 ℃, the 10 minutes enzymes that go out after finishing;
(11) spraying drying: enzymolysis solution is carried out spraying drying, and air outlet temperature is 85 ℃, and moisture content is below 5%, and dried product is avenabeta glucosan.
2. the preparation method of avenabeta glucosan according to claim 1, the enzyme activity that it is characterized in that used heat-resistant alpha-amylase in the process is 20, and 000U/mL, the enzyme activity of liquid saccharifying enzyme are 50, and 000U/mL, the enzyme activity of beta-glucanase are 40U/mL.
3. the preparation method of avenabeta glucosan according to claim 1 is characterized in that the avenabeta glucosan that makes is a white powder, and relative molecular mass is 5000～10000, and enteron aisle and ight soil bifidus bacillus, lactobacillus are had the promotion proliferation function.
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Cited By (1)
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| CN109536546A (en) * | 2018-11-28 | 2019-03-29 | 上海晟薇生物科技有限公司 | A kind of preparation method and application of small molecular amount avenabeta glucosan |
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| CN101602816B (en) * | 2009-06-18 | 2011-06-15 | 广东省食品工业研究所 | Method for extracting water-soluble beta-glucan with high purity from grain bran |
| CN101617787B (en) * | 2009-07-27 | 2012-07-11 | 中国科学院西北高原生物研究所 | Process for continuously extracting a plurality of products from highland barley |
| US8333986B2 (en) | 2009-08-10 | 2012-12-18 | A.G.V. Products Corp. | Oligo-saccharide enhanced oat-based drink for treating hyperlipidaemia and hyperglycemia and improving gastrointestinal function and process for preparing the same by tri-enzyme hydrolysis and micro-particle milling |
| TWI441600B (en) | 2009-08-10 | 2014-06-21 | V Products Corp Ag | Oligo-saccharide enhanced oat-based drink for treating hyperlipidaemia and hyperglycemia and improving gastrointestinal function and process for preparing the same by tri-enzyme hydrolysis and micro-particle milling |
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| CN103204952A (en) * | 2013-03-18 | 2013-07-17 | 南通博硕生物工程有限公司 | Oat glucan extraction method |
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