CN100396702C - Process for extracting pholita adiposa polysaccharide - Google Patents
Process for extracting pholita adiposa polysaccharide Download PDFInfo
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- CN100396702C CN100396702C CNB2006100423209A CN200610042320A CN100396702C CN 100396702 C CN100396702 C CN 100396702C CN B2006100423209 A CNB2006100423209 A CN B2006100423209A CN 200610042320 A CN200610042320 A CN 200610042320A CN 100396702 C CN100396702 C CN 100396702C
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Abstract
The present invention discloses a technology for extracting pholita adiposa polysaccharide, which comprises technology steps of material preparation, supersonic extraction or microwave extraction, concentration, alcohol sedimentation, collection, desiccation, packing, preservation, etc. The technology of the present invention has the advantages of simple operation and practicality; when the technology of the present invention is used for extracting the pholita adiposa polysaccharide, the extraction time is saved, the extraction rate is high, and the extract has obvious functions of improving animal immunity, resisting tumors, resisting oxygen deficiency, reducing blood sugar, etc.
Description
Technical field
The present invention relates to a kind of extracting method of polysaccharide, particularly relate to a kind of extracting method of pholiota adiosapose polysaccharide.
Background technology
Yellow umbrella [Pholiota adipdsa (Fr) Quel] is wild a kind of rare food medicine dual-purpose fungi on willow, and sporophore is tender and crisp good to eat, nutritious.The treatment that is usually used in Digestive tract illness such as cancer of the stomach among the people has eliminate indigestion and helps digestion, clear refreshing restoring consciouness and antibacterial anti-infectious effect.Learn that through data check and test the main pharmacodynamics composition of yellow umbrella is a polysaccharide material.At present, wild yellow umbrella is very rare, and applicant in 1994 has been carried out the large-scale promotion cultivation in many provinces and regions after the domestication cultivates success first at home at present.
Prove that according to data check and applicant experimental study the pholiota adiosapose polysaccharide that extracts has the immunizing power that significantly improves animal, antitumor, hypoxia tolerance, function such as hypoglycemic from yellow umbrella.Pholiota adiosapose polysaccharide is a pure natural substance, can foodstuff additive, forms such as medicinal granules, oral liquid, capsule, dripping pill, soft capsule are prepared into healthcare products or medicine, to satisfy people's back to nature, to improve healthy demand.The paper that applicant is delivered has been introduced use hot water extraction and has been extracted pholiota adiosapose polysaccharide, but this extracting method exists extraction time long, the shortcoming that extraction yield is low.
Summary of the invention
The present invention provides a kind of extracting method of pholiota adiosapose polysaccharide in order to solve the deficiency of above existence.The practicality simple to operate of this method, the pholiota adiosapose polysaccharide that adopts this method to extract is saved extraction time, the extraction yield height.
This method is used supersound extraction, and comprise material pre-treatment, supersound extraction, concentrate, steps such as alcohol precipitation, collection, drying, packing preservation.Also can use microwave extraction, comprise pre-treatment, microwave extraction, concentrate, steps such as alcohol precipitation, collection, drying, packing preservation.
Embodiment
Further specify the present invention below by specific embodiment.
Embodiment 1:
1) material pre-treatment: choose the yellow destroying angel 10g that solid culture is produced, through pulverize, degreasing, added 300 water logging bubble 2 hours, to be extracted;
2) supersound extraction: selected frequency was the ultrasonic generator of 25KHz, to above-mentioned through pretreated extraction material ultrasonic 30 minutes.By whizzer under 3000 rev/mins condition centrifugal 10 minutes, handled thing is carried out solid-liquid separation, supernatant liquor is concentrated after abandoning solid substance;
3) concentrate: carry out concentrating under reduced pressure at 60 ℃ to collecting supernatant liquor, be concentrated into 1/4 of original volume and get final product;
4) alcohol precipitation: adopt the ethanol of 95% concentration that above-mentioned concentrated solution is mixed, form throw out, be deposited in container base owing to polysaccharide is insoluble to ethanol;
5) collect: the concentrated solution alcohol precipitation is after 12 hours, under 3000 rev/mins of rotating speeds centrifugal 10 minutes, collects Centrifuge Cup bottom polysaccharide;
6) drying: the polysaccharide of collecting is put in the vacuum drying oven, 50 ℃ of vacuum-dryings;
7) packing is preserved: after dried polysaccharide is ground into fine powder, seals preservation in the Aluminium Foil Package of packing into the pack or carry out product processing.
Embodiment 2:
1) material pre-treatment: the pholiota adiposa mycelium that submerged fermentation is cultivated is through add water by 5 times of weight of weight in wet base after separation, the rinsing from nutrient solution, and is to be extracted;
2) supersound extraction: selected frequency was the ultrasonic generator of 40KHz, to above-mentioned through pretreated extraction material ultrasonic 15 minutes.By whizzer under 3000 rev/mins condition centrifugal 10 minutes, handled thing is carried out solid-liquid separation, supernatant liquor is concentrated after abandoning solid substance;
3) concentrate: carry out concentrating under reduced pressure at 50 ℃ to collecting supernatant liquor, be concentrated into 1/4 of original volume and get final product;
4) alcohol precipitation: adopt the ethanol of 95% concentration that above-mentioned concentrated solution is mixed, form throw out, be deposited in container base owing to polysaccharide is insoluble to ethanol;
5) collect: the concentrated solution alcohol precipitation is after 12 hours, under 3000 rev/mins of rotating speeds centrifugal 10 minutes, collects Centrifuge Cup bottom polysaccharide;
6) drying: the polysaccharide of collecting is put in the vacuum drying oven, 50 ℃ of vacuum-dryings;
7) packing is preserved: after dried polysaccharide is ground into fine powder, seals preservation in the Aluminium Foil Package of packing into the pack or carry out product processing.
Embodiment 3:
1) material pre-treatment: choose the yellow destroying angel 10g that solid culture is produced, through pulverize, degreasing, added 300 water logging bubble 2 hours, to be extracted;
2) microwave extraction: selected frequency is that 2450MHz, power are the microwave generator of 700w, carries out microwave treatment 5 minutes (transfer to 70% microwave power) to above-mentioned through pretreated extraction material.Handled thing under 3000 rev/mins condition centrifugal 10 minutes carries out solid-liquid separation, supernatant liquor is concentrated after abandoning solid substance;
3) concentrate: carry out concentrating under reduced pressure at 60 ℃ to collecting supernatant liquor, be concentrated into 1/4 of original volume and get final product;
4) alcohol precipitation: adopt the ethanol of 95% concentration that above-mentioned concentrated solution is mixed, form throw out, be deposited in container base owing to polysaccharide is insoluble to ethanol;
5) collect: the concentrated solution alcohol precipitation is after 12 hours, under 3000 rev/mins of rotating speeds centrifugal 10 minutes, collects Centrifuge Cup bottom polysaccharide;
6) drying: the polysaccharide of collecting is put in the vacuum drying oven, 50 ℃ of vacuum-dryings;
7) packing is preserved: after dried polysaccharide is ground into fine powder, seals preservation in the Aluminium Foil Package of packing into the pack or carry out product processing.
Embodiment 4:
1) material pre-treatment: the pholiota adiposa mycelium that submerged fermentation is cultivated is through add water by 5 times of weight of weight in wet base after separation, the rinsing from nutrient solution, and is to be extracted;
2) microwave extraction: selected frequency is that 2450MHz, power are the microwave generator of 700w, carries out microwave treatment 5 minutes (transfer to 70% microwave power) to above-mentioned through pretreated extraction material.Handled thing under 3000 rev/mins condition centrifugal 10 minutes carries out solid-liquid separation, supernatant liquor is concentrated after abandoning solid substance;
3) concentrate: carry out concentrating under reduced pressure at 50 ℃ to collecting supernatant liquor, be concentrated into 1/4 of original volume and get final product;
4) alcohol precipitation: adopt the ethanol of 95% concentration that above-mentioned concentrated solution is mixed, form throw out, be deposited in container base owing to polysaccharide is insoluble to ethanol;
5) collect: the concentrated solution alcohol precipitation is after 12 hours, under 3000 rev/mins of rotating speeds centrifugal 10 minutes, collects Centrifuge Cup bottom polysaccharide;
6) drying: the polysaccharide of collecting is put in the vacuum drying oven, 50 ℃ of vacuum-dryings;
7) packing is preserved: after dried polysaccharide is ground into fine powder, seals preservation in the Aluminium Foil Package of packing into the pack or carry out product processing.
Claims (4)
1. the extracting method of a pholiota adiosapose polysaccharide is characterized in that comprising material pre-treatment, supersound extraction, concentrates, alcohol precipitation, collection, drying, packing store method step, and concrete steps are as follows:
1) material pre-treatment: the yellow destroying angel that solid culture is produced through pulverizing, degreasing, adds 1: the water logging bubble of 20-40 1-2 hour, and to be extracted;
2) supersound extraction: selected frequency is the ultrasonic generator of 25-40KHz, to above-mentioned through pretreated extraction material supersound process 15-30 minute.By whizzer under 3000 rev/mins condition centrifugal 10 minutes, carry out solid-liquid separation, supernatant liquor is concentrated after abandoning solid substance;
3) concentrate:, be concentrated into 1/4 of original volume and get final product below 70 ℃ the supernatant liquor of collecting being carried out concentrating under reduced pressure;
4) alcohol precipitation: adopt the ethanol of 95% concentration that above-mentioned concentrated solution is mixed, form throw out, be deposited in container base owing to polysaccharide is insoluble to ethanol;
5) collect: the concentrated solution alcohol precipitation is after 12 hours, under 3000 rev/mins of rotating speeds centrifugal 10 minutes, collects Centrifuge Cup bottom polysaccharide;
6) drying: the polysaccharide of collecting is put in the vacuum drying oven, in vacuum-drying below 60 ℃;
7) packing is preserved: after dried polysaccharide is ground into fine powder, seals preservation in the Aluminium Foil Package of packing into the pack or carry out product processing.
2. the extracting method of a pholiota adiosapose polysaccharide is characterized in that comprising material pre-treatment, supersound extraction, concentrates, alcohol precipitation, collection, drying, packing store method step, and concrete steps are as follows:
1) material pre-treatment: the pholiota adiposa mycelium that submerged fermentation is cultivated is through add water by 5 times of weight of weight in wet base after separation, the rinsing from nutrient solution, and is to be extracted;
2) supersound extraction: selected frequency is the ultrasonic generator of 25-40KHz, to above-mentioned through the pretreated ultrasonic 15-30 of extraction material minute.By whizzer under 3000 rev/mins condition centrifugal 10 minutes, handled thing is carried out solid-liquid separation, supernatant liquor is concentrated after abandoning solid substance;
3) concentrate:, be concentrated into 1/4 of original volume and get final product below 70 ℃ the supernatant liquor of collecting being carried out concentrating under reduced pressure;
4) alcohol precipitation: adopt the ethanol of 95% concentration that above-mentioned concentrated solution is mixed, form throw out, be deposited in container base owing to polysaccharide is insoluble to ethanol;
5) collect: the concentrated solution alcohol precipitation is after 12 hours, under 3000 rev/mins of rotating speeds centrifugal 10 minutes, collects Centrifuge Cup bottom polysaccharide;
6) drying: the polysaccharide of collecting is put in the vacuum drying oven, 60 ℃ of following vacuum-dryings;
7) packing is preserved: after dried polysaccharide is ground into fine powder, seals preservation in the Aluminium Foil Package of packing into the pack or carry out product processing.
3. the extracting method of a pholiota adiosapose polysaccharide is characterized in that comprising material pre-treatment, microwave extraction, concentrates, alcohol precipitation, collection, drying, packing store method step, and concrete steps are as follows:
1) material pre-treatment: the yellow destroying angel that solid culture is produced through pulverizing, degreasing, adds 1: the water logging bubble of 20-40 1-2 hour, and to be extracted;
2) microwave extraction: selected frequency is that 2450MHz, power are the microwave generator of 700w, carry out microwave treatment through pretreated extraction material and transferred to 70% microwave power in 5 minutes above-mentioned, handled thing under 3000 rev/mins condition centrifugal 10 minutes, carry out solid-liquid separation, supernatant liquor is concentrated after abandoning round;
3) concentrate:, be concentrated into 1/4 of original volume and get final product below 70 ℃ the supernatant liquor of collecting being carried out concentrating under reduced pressure;
4) alcohol precipitation: adopt the ethanol of 95% concentration that above-mentioned concentrated solution is mixed, form throw out, be deposited in container base owing to polysaccharide is insoluble to ethanol;
5) collect: the concentrated solution alcohol precipitation is after 12 hours, under 3000 rev/mins of rotating speeds centrifugal 10 minutes, collects Centrifuge Cup bottom polysaccharide;
6) drying: the polysaccharide of collecting is put in the vacuum drying oven, in vacuum-drying below 60 ℃;
7) packing is preserved: after dried polysaccharide is ground into fine powder, seals preservation in the Aluminium Foil Package of packing into the pack or carry out product processing.
4. the extracting method of a pholiota adiosapose polysaccharide is characterized in that comprising material pre-treatment, microwave extraction, concentrates, alcohol precipitation, collection, drying, packing store method step, and concrete steps are as follows:
1) material pre-treatment: the pholiota adiposa mycelium that submerged fermentation is cultivated is through add water by 5 times of weight of weight in wet base after separation, the rinsing from nutrient solution, and is to be extracted;
2) microwave extraction: selected frequency is that 2450MHz, power are the microwave generator of 700w, carry out microwave treatment through pretreated extraction material and transferred to 70% microwave power in 5 minutes above-mentioned, handled thing under 3000 rev/mins condition centrifugal 10 minutes, carry out solid-liquid separation, supernatant liquor is concentrated after abandoning solid substance;
3) concentrate:, be concentrated into 1/4 of original volume and get final product below 70 ℃ the supernatant liquor of collecting being carried out concentrating under reduced pressure;
4) alcohol precipitation: adopt the ethanol of 95% concentration that above-mentioned concentrated solution is mixed, form throw out, be deposited in container base owing to polysaccharide is insoluble to ethanol;
5) collect: the concentrated solution alcohol precipitation is after 12 hours, under 3000 rev/mins of rotating speeds centrifugal 10 minutes, collects Centrifuge Cup bottom polysaccharide;
6) drying: the polysaccharide of collecting is put in the vacuum drying oven, 60 ℃ of following vacuum-dryings;
7) packing is preserved: after dried polysaccharide is ground into fine powder, seals preservation in the Aluminium Foil Package of packing into the pack or carry out product processing.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100423209A CN100396702C (en) | 2006-01-25 | 2006-01-25 | Process for extracting pholita adiposa polysaccharide |
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| CNB2006100423209A CN100396702C (en) | 2006-01-25 | 2006-01-25 | Process for extracting pholita adiposa polysaccharide |
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| CN100396702C true CN100396702C (en) | 2008-06-25 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101735952B (en) * | 2008-11-04 | 2011-09-21 | 中国农业科学院农业资源与农业区划研究所 | Novel strain of pholiota adiosapose |
| CN101525646B (en) * | 2009-03-06 | 2011-11-09 | 苏源 | Pnoliota adiposa mycelium selenium enrichment cultivation and technology for extracting pnoliota adiposa selenium polysaccharide |
| CN101580551B (en) * | 2009-06-23 | 2011-11-30 | 宽甸玉竹农副产品有限公司 | Method for extracting polygonatum odoratum polysaccharide |
| CN102835240A (en) * | 2012-09-03 | 2012-12-26 | 苏延友 | Cultivation method of selenium-enriched Codonopsis lanceolata, as well as extraction method and application of selenium-containing polysaccharide of selenium-enriched Codonopsis lanceolata |
| CN103285045B (en) * | 2013-05-30 | 2015-03-04 | 北京市农林科学院 | Pholiota adiposa protein extract and anti-tumor application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1687122A (en) * | 2005-03-23 | 2005-10-26 | 江苏大学 | Purple laver protein and polysaccharide product, and method of ultrasonic assistant extraction |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1687122A (en) * | 2005-03-23 | 2005-10-26 | 江苏大学 | Purple laver protein and polysaccharide product, and method of ultrasonic assistant extraction |
Non-Patent Citations (10)
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| 正交试验法优选黄伞多糖提取工艺的研究. 苏延友，苏延峰，杨杰.食用菌，第1期. 2004 |
| 正交试验法优选黄伞多糖提取工艺的研究. 苏延友，苏延峰，杨杰.食用菌，第1期. 2004 * |
| 超声波法提取灵芝多糖的研究. 李钟玉，张京东，李临生.中国食用菌，第23卷第2期. 2004 |
| 超声波法提取灵芝多糖的研究. 李钟玉，张京东，李临生.中国食用菌，第23卷第2期. 2004 * |
| 超声波法提取香菇多糖的研究. 王亚飞，毕红梅.内蒙古科技与经济，第14期. 2004 |
| 超声波法提取香菇多糖的研究. 王亚飞，毕红梅.内蒙古科技与经济，第14期. 2004 * |
| 黄伞多糖的提取及对小鼠腹腔巨噬细胞的激活效应研究. 苏延友，康莉，杨志孝，刘爱华.泰山医学院学报，第25卷第1期. 2004 |
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| 黄伞菌丝体多糖提取及其抗疲劳活性的研究. 黄清荣，辛晓林，钟旭生，张红艳，史云卿.食品研究与开发，第26卷第6期. 2005 |
| 黄伞菌丝体多糖提取及其抗疲劳活性的研究. 黄清荣，辛晓林，钟旭生，张红艳，史云卿.食品研究与开发，第26卷第6期. 2005 * |
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