CL2020003271A1 - Intercambio de reactivo en un instrumento - Google Patents
Intercambio de reactivo en un instrumentoInfo
- Publication number
- CL2020003271A1 CL2020003271A1 CL2020003271A CL2020003271A CL2020003271A1 CL 2020003271 A1 CL2020003271 A1 CL 2020003271A1 CL 2020003271 A CL2020003271 A CL 2020003271A CL 2020003271 A CL2020003271 A CL 2020003271A CL 2020003271 A1 CL2020003271 A1 CL 2020003271A1
- Authority
- CL
- Chile
- Prior art keywords
- reagent
- base
- polynucleotide
- flow cell
- management system
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/0002—Inspection of images, e.g. flaw detection
- G06T7/0012—Biomedical image inspection
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00389—Feeding through valves
- B01J2219/00391—Rotary valves
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00389—Feeding through valves
- B01J2219/00391—Rotary valves
- B01J2219/00394—Rotary valves in multiple arrangements
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/0054—Means for coding or tagging the apparatus or the reagents
- B01J2219/00572—Chemical means
- B01J2219/00576—Chemical means fluorophore
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00599—Solution-phase processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00693—Means for quality control
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
- B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/30—Subject of image; Context of image processing
- G06T2207/30004—Biomedical image processing
- G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Medical Informatics (AREA)
- General Physics & Mathematics (AREA)
- Radiology & Medical Imaging (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Quality & Reliability (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Theoretical Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Image Processing (AREA)
Abstract
Un método incluye hacer fluir un reactivo de incorporación a través de un sistema de gestión de reactivos y una celda de flujo de un instrumento. La celda de flujo tiene un primer polinucleótido colocado en su interior. El reactivo de incorporación añade una primera base a una secuencia de bases. La secuencia de bases incluye un segundo polinucleótido complementario al primer polinucleótido. Se captura una imagen de una señal de identificación que emana de la primera base después de que la primera base se haya añadido al segundo polinucleótido. Se hace fluir un reactivo de escisión a través del sistema de gestión de reactivos y la celda de flujo para eliminar un primer terminador de la primera base con el fin de permitir que una base posterior en la secuencia de bases se añada al segundo polinucleótido. Se hace fluir un reactivo tampón a través del sistema de gestión de reactivos y la celda de flujo en una pluralidad de ciclos de direcciones de flujo consecutivas hacia adelante y hacia atrás.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962863444P | 2019-06-19 | 2019-06-19 |
Publications (1)
Publication Number | Publication Date |
---|---|
CL2020003271A1 true CL2020003271A1 (es) | 2021-06-04 |
Family
ID=73799087
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CL2020003271A CL2020003271A1 (es) | 2019-06-19 | 2020-12-17 | Intercambio de reactivo en un instrumento |
Country Status (17)
Country | Link |
---|---|
US (2) | US11911765B2 (es) |
EP (1) | EP3987060A4 (es) |
JP (1) | JP2022537853A (es) |
KR (1) | KR20220022086A (es) |
CN (2) | CN213327626U (es) |
AU (1) | AU2020296852A1 (es) |
BR (1) | BR112020026645A2 (es) |
CA (1) | CA3104133A1 (es) |
CL (1) | CL2020003271A1 (es) |
CO (1) | CO2020015882A2 (es) |
CR (1) | CR20200648A (es) |
IL (1) | IL279438A (es) |
MX (1) | MX2020014057A (es) |
PE (1) | PE20220208A1 (es) |
SG (1) | SG11202012992RA (es) |
TW (1) | TW202104901A (es) |
WO (1) | WO2020257151A1 (es) |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0985142A4 (en) * | 1997-05-23 | 2006-09-13 | Lynx Therapeutics Inc | SYSTEM AND APPARATUS FOR THE SEQUENTIAL TREATMENT OF ANALYTES |
EP2018622B1 (en) * | 2006-03-31 | 2018-04-25 | Illumina, Inc. | Systems for sequence by synthesis analysis |
EP2126765B1 (en) | 2007-01-26 | 2011-08-24 | Illumina Inc. | Nucleic acid sequencing system and method |
WO2013096692A1 (en) * | 2011-12-21 | 2013-06-27 | Illumina, Inc. | Apparatus and methods for kinetic analysis and determination of nucleic acid sequences |
CN104053498B (zh) * | 2012-01-13 | 2017-05-03 | 皇家飞利浦有限公司 | 使用在线栅上再循环的试剂的dna测序 |
WO2014110287A1 (en) * | 2013-01-09 | 2014-07-17 | Caerus Molecular Diagnostics | Sequencing nucleic acids by enzyme activation |
CN108603227A (zh) * | 2015-11-18 | 2018-09-28 | 卡利姆·U·米尔 | 超分辨率测序 |
GB201704747D0 (en) | 2017-01-05 | 2017-05-10 | Illumina Inc | Reagent mixing system and methods |
GB201704754D0 (en) | 2017-01-05 | 2017-05-10 | Illumina Inc | Kinetic exclusion amplification of nucleic acid libraries |
EP3606673A4 (en) * | 2017-04-06 | 2020-10-28 | Illumina, Inc. | METHOD AND DEVICE FOR IDENTIFICATION OF FLUID TRAVEL POSITIONS |
WO2019221913A1 (en) * | 2018-05-15 | 2019-11-21 | Illumina, Inc. | Flow cell with flexible connection |
-
2020
- 2020-06-02 TW TW109118502A patent/TW202104901A/zh unknown
- 2020-06-16 EP EP20825560.4A patent/EP3987060A4/en active Pending
- 2020-06-16 US US16/902,826 patent/US11911765B2/en active Active
- 2020-06-16 SG SG11202012992RA patent/SG11202012992RA/en unknown
- 2020-06-16 CR CR20200648A patent/CR20200648A/es unknown
- 2020-06-16 BR BR112020026645A patent/BR112020026645A2/pt unknown
- 2020-06-16 JP JP2020573009A patent/JP2022537853A/ja active Pending
- 2020-06-16 MX MX2020014057A patent/MX2020014057A/es unknown
- 2020-06-16 AU AU2020296852A patent/AU2020296852A1/en active Pending
- 2020-06-16 KR KR1020207037436A patent/KR20220022086A/ko unknown
- 2020-06-16 WO PCT/US2020/037864 patent/WO2020257151A1/en active Application Filing
- 2020-06-16 CA CA3104133A patent/CA3104133A1/en active Pending
- 2020-06-16 PE PE2020002096A patent/PE20220208A1/es unknown
- 2020-06-19 CN CN202021158057.1U patent/CN213327626U/zh active Active
- 2020-06-19 CN CN202010565550.3A patent/CN112111567A/zh active Pending
- 2020-12-14 IL IL279438A patent/IL279438A/en unknown
- 2020-12-17 CO CONC2020/0015882A patent/CO2020015882A2/es unknown
- 2020-12-17 CL CL2020003271A patent/CL2020003271A1/es unknown
-
2023
- 2023-12-04 US US18/527,992 patent/US20240100532A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
AU2020296852A1 (en) | 2021-01-21 |
PE20220208A1 (es) | 2022-02-01 |
MX2020014057A (es) | 2021-05-27 |
CN112111567A (zh) | 2020-12-22 |
KR20220022086A (ko) | 2022-02-24 |
JP2022537853A (ja) | 2022-08-31 |
EP3987060A4 (en) | 2023-05-31 |
CA3104133A1 (en) | 2020-12-19 |
CN213327626U (zh) | 2021-06-01 |
BR112020026645A2 (pt) | 2022-01-18 |
WO2020257151A1 (en) | 2020-12-24 |
TW202104901A (zh) | 2021-02-01 |
EP3987060A1 (en) | 2022-04-27 |
IL279438A (en) | 2021-01-31 |
US11911765B2 (en) | 2024-02-27 |
CR20200648A (es) | 2021-04-30 |
US20200398278A1 (en) | 2020-12-24 |
US20240100532A1 (en) | 2024-03-28 |
CO2020015882A2 (es) | 2021-05-31 |
SG11202012992RA (en) | 2021-01-28 |
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