BR112020026645A2 - Método e instrumento para troca de reagente - Google Patents
Método e instrumento para troca de reagenteInfo
- Publication number
- BR112020026645A2 BR112020026645A2 BR112020026645A BR112020026645A BR112020026645A2 BR 112020026645 A2 BR112020026645 A2 BR 112020026645A2 BR 112020026645 A BR112020026645 A BR 112020026645A BR 112020026645 A BR112020026645 A BR 112020026645A BR 112020026645 A2 BR112020026645 A2 BR 112020026645A2
- Authority
- BR
- Brazil
- Prior art keywords
- reagent
- base
- polynucleotide
- flow cell
- instrument
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/0002—Inspection of images, e.g. flaw detection
- G06T7/0012—Biomedical image inspection
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00389—Feeding through valves
- B01J2219/00391—Rotary valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00389—Feeding through valves
- B01J2219/00391—Rotary valves
- B01J2219/00394—Rotary valves in multiple arrangements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/0054—Means for coding or tagging the apparatus or the reagents
- B01J2219/00572—Chemical means
- B01J2219/00576—Chemical means fluorophore
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00599—Solution-phase processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00693—Means for quality control
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
- B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/30—Subject of image; Context of image processing
- G06T2207/30004—Biomedical image processing
- G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Medical Informatics (AREA)
- General Physics & Mathematics (AREA)
- Radiology & Medical Imaging (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Quality & Reliability (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Theoretical Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Image Processing (AREA)
Abstract
método e instrumento para troca de reagente. um método inclui fluir um reagente de incorporação através de um sistema de gerenciamento de reagente e uma célula de fluxo de um instrumento. a célula de fluxo tendo um primeiro polinucleotídeo posicionado na mesma. o reagente de incorporação adicionando uma primeira base sobre uma sequência de bases. a sequência de bases inclui um segundo polinucleotídeo complementar ao primeiro polinucleotídeo. uma imagem de um sinal de identificação emanando a partir da primeira base é capturada após a primeira base ter sido adicionada sobre o segundo polinucleotídeo. um reagente de clivagem é fluído através do sistema de gerenciamento de reagente e célula de fluxo para remover um primeiro terminador a partir da primeira base para permitir que uma base subsequente na sequência de bases seja adicionada ao segundo polinucleotídeo. um reagente de tampão é fluído através do sistema de gerenciamento de reagente e célula de fluxo em uma pluralidade de ciclos de direções de fluxo avançado e inverso consecutivos.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962863444P | 2019-06-19 | 2019-06-19 | |
PCT/US2020/037864 WO2020257151A1 (en) | 2019-06-19 | 2020-06-16 | Reagent exchange in an instrument |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112020026645A2 true BR112020026645A2 (pt) | 2022-01-18 |
Family
ID=73799087
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112020026645A BR112020026645A2 (pt) | 2019-06-19 | 2020-06-16 | Método e instrumento para troca de reagente |
Country Status (17)
Country | Link |
---|---|
US (2) | US11911765B2 (pt) |
EP (1) | EP3987060A4 (pt) |
JP (1) | JP2022537853A (pt) |
KR (1) | KR20220022086A (pt) |
CN (2) | CN213327626U (pt) |
AU (1) | AU2020296852A1 (pt) |
BR (1) | BR112020026645A2 (pt) |
CA (1) | CA3104133A1 (pt) |
CL (1) | CL2020003271A1 (pt) |
CO (1) | CO2020015882A2 (pt) |
CR (1) | CR20200648A (pt) |
IL (1) | IL279438A (pt) |
MX (1) | MX2020014057A (pt) |
PE (1) | PE20220208A1 (pt) |
SG (1) | SG11202012992RA (pt) |
TW (1) | TW202104901A (pt) |
WO (1) | WO2020257151A1 (pt) |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0985142A4 (en) * | 1997-05-23 | 2006-09-13 | Lynx Therapeutics Inc | SYSTEM AND APPARATUS FOR THE SEQUENTIAL TREATMENT OF ANALYTES |
EP2018622B1 (en) * | 2006-03-31 | 2018-04-25 | Illumina, Inc. | Systems for sequence by synthesis analysis |
EP2126765B1 (en) | 2007-01-26 | 2011-08-24 | Illumina Inc. | Nucleic acid sequencing system and method |
WO2013096692A1 (en) * | 2011-12-21 | 2013-06-27 | Illumina, Inc. | Apparatus and methods for kinetic analysis and determination of nucleic acid sequences |
CN104053498B (zh) * | 2012-01-13 | 2017-05-03 | 皇家飞利浦有限公司 | 使用在线栅上再循环的试剂的dna测序 |
WO2014110287A1 (en) * | 2013-01-09 | 2014-07-17 | Caerus Molecular Diagnostics | Sequencing nucleic acids by enzyme activation |
CN108603227A (zh) * | 2015-11-18 | 2018-09-28 | 卡利姆·U·米尔 | 超分辨率测序 |
GB201704747D0 (en) | 2017-01-05 | 2017-05-10 | Illumina Inc | Reagent mixing system and methods |
GB201704754D0 (en) | 2017-01-05 | 2017-05-10 | Illumina Inc | Kinetic exclusion amplification of nucleic acid libraries |
EP3606673A4 (en) * | 2017-04-06 | 2020-10-28 | Illumina, Inc. | METHOD AND DEVICE FOR IDENTIFICATION OF FLUID TRAVEL POSITIONS |
WO2019221913A1 (en) * | 2018-05-15 | 2019-11-21 | Illumina, Inc. | Flow cell with flexible connection |
-
2020
- 2020-06-02 TW TW109118502A patent/TW202104901A/zh unknown
- 2020-06-16 EP EP20825560.4A patent/EP3987060A4/en active Pending
- 2020-06-16 US US16/902,826 patent/US11911765B2/en active Active
- 2020-06-16 SG SG11202012992RA patent/SG11202012992RA/en unknown
- 2020-06-16 CR CR20200648A patent/CR20200648A/es unknown
- 2020-06-16 BR BR112020026645A patent/BR112020026645A2/pt unknown
- 2020-06-16 JP JP2020573009A patent/JP2022537853A/ja active Pending
- 2020-06-16 MX MX2020014057A patent/MX2020014057A/es unknown
- 2020-06-16 AU AU2020296852A patent/AU2020296852A1/en active Pending
- 2020-06-16 KR KR1020207037436A patent/KR20220022086A/ko unknown
- 2020-06-16 WO PCT/US2020/037864 patent/WO2020257151A1/en active Application Filing
- 2020-06-16 CA CA3104133A patent/CA3104133A1/en active Pending
- 2020-06-16 PE PE2020002096A patent/PE20220208A1/es unknown
- 2020-06-19 CN CN202021158057.1U patent/CN213327626U/zh active Active
- 2020-06-19 CN CN202010565550.3A patent/CN112111567A/zh active Pending
- 2020-12-14 IL IL279438A patent/IL279438A/en unknown
- 2020-12-17 CO CONC2020/0015882A patent/CO2020015882A2/es unknown
- 2020-12-17 CL CL2020003271A patent/CL2020003271A1/es unknown
-
2023
- 2023-12-04 US US18/527,992 patent/US20240100532A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
AU2020296852A1 (en) | 2021-01-21 |
CL2020003271A1 (es) | 2021-06-04 |
PE20220208A1 (es) | 2022-02-01 |
MX2020014057A (es) | 2021-05-27 |
CN112111567A (zh) | 2020-12-22 |
KR20220022086A (ko) | 2022-02-24 |
JP2022537853A (ja) | 2022-08-31 |
EP3987060A4 (en) | 2023-05-31 |
CA3104133A1 (en) | 2020-12-19 |
CN213327626U (zh) | 2021-06-01 |
WO2020257151A1 (en) | 2020-12-24 |
TW202104901A (zh) | 2021-02-01 |
EP3987060A1 (en) | 2022-04-27 |
IL279438A (en) | 2021-01-31 |
US11911765B2 (en) | 2024-02-27 |
CR20200648A (es) | 2021-04-30 |
US20200398278A1 (en) | 2020-12-24 |
US20240100532A1 (en) | 2024-03-28 |
CO2020015882A2 (es) | 2021-05-31 |
SG11202012992RA (en) | 2021-01-28 |
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