CL2020000953A1 - Sistemas y métodos para la reprogramación celular de una célula vegetal. - Google Patents

Sistemas y métodos para la reprogramación celular de una célula vegetal.

Info

Publication number
CL2020000953A1
CL2020000953A1 CL2020000953A CL2020000953A CL2020000953A1 CL 2020000953 A1 CL2020000953 A1 CL 2020000953A1 CL 2020000953 A CL2020000953 A CL 2020000953A CL 2020000953 A CL2020000953 A CL 2020000953A CL 2020000953 A1 CL2020000953 A1 CL 2020000953A1
Authority
CL
Chile
Prior art keywords
plant
embryogenesis
reprogramming
cells
cell
Prior art date
Application number
CL2020000953A
Other languages
English (en)
Inventor
Tim Fox
William James Gordon-Kamm
Rachel Carol Huegel
Jon Aaron Tucker Reinders
Huaxun Ye
Keith S Lowe
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of CL2020000953A1 publication Critical patent/CL2020000953A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

EL DESARROLLO Y DESTINO DE CÉLULAS VEGETALES SE ALTERA TRATANDO LAS CÉLULAS CON FACTORES DE REPROGRAMACIÓN CELULAR. SE USAN GENES DE DESARROLLO MORFOGÉNICOS INDUCTORES DE EMBRIOGÉNESIS COMO FACTORES DE REPROGRAMACIÓN CELULAR, QUE COMPRENDEN ESPECÍFICAMENTE POLIPÉPTIDOS O POLINUCLEÓTIDOS QUE CODIFICAN PARA PRODUCTOS GÉNICOS PARA GENERAR HAPLOIDES DUPLICADOS O PLANTAS HAPLOIDES A PARTIR DE GAMETOS. LAS MICROSPORAS DE MAÍZ TRATADAS PONIENDO EN CONTACTO LAS CÉLULAS AISLADAS CON UN GEN DE DESARROLLO MORFOGÉNICO INDUCTOR DE EMBRIOGÉNESIS EXÓGENO, PURIFICADO, RECOMBINANTE DAN COMO RESULTADO EMBRIOGÉNESIS. LOS GAMETOS DE UNA PLANTA DE MAÍZ SE DESARROLLAN PARA DAR EMBRIOIDES CUANDO SE TRANSFORMAN CON UN CONSTRUCTO GENÉTICO QUE INCLUYE ELEMENTOS REGULADORES Y GENES ESTRUCTURALES CAPACES DE ACTUAR EN UN MODO DE CASCADA PARA ALTERAR EL DESTINO CELULAR DE CÉLULAS VEGETALES. SE USAN PROTEÍNAS MORFOGÉNICAS DEL DESARROLLO EXPRESADAS A PARTIR DE UN CONSTRUCTO GENÉTICO PARA MÉTODOS DE TRATAMIENTO EX SITU Y PARA LA REPROGRAMACIÓN CELULAR IN PLANTA.
CL2020000953A 2017-10-13 2020-04-08 Sistemas y métodos para la reprogramación celular de una célula vegetal. CL2020000953A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201762572007P 2017-10-13 2017-10-13

Publications (1)

Publication Number Publication Date
CL2020000953A1 true CL2020000953A1 (es) 2020-09-11

Family

ID=64051788

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2020000953A CL2020000953A1 (es) 2017-10-13 2020-04-08 Sistemas y métodos para la reprogramación celular de una célula vegetal.

Country Status (11)

Country Link
US (2) US11447786B2 (es)
EP (1) EP3694308A1 (es)
CN (1) CN111465321B (es)
AU (1) AU2018347545A1 (es)
BR (1) BR112020007343A2 (es)
CA (1) CA3078819A1 (es)
CL (1) CL2020000953A1 (es)
IL (1) IL273710A (es)
MX (1) MX2020004138A (es)
WO (1) WO2019075295A1 (es)
ZA (1) ZA202002527B (es)

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EP4002993A4 (en) * 2019-07-30 2024-01-10 Pairwise Plants Services Inc MORPHOGENE REGULATORS AND METHODS OF USE THEREOF
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EP3827665A1 (en) * 2019-11-26 2021-06-02 Consejo Superior de Investigaciones Científicas (CSIC) Mammal kinase inhibitors to promote in vitro embryogenesis induction of plants
AU2021220736A1 (en) * 2020-02-12 2022-07-21 Pioneer Hi-Bred International, Inc. Cas-mediated homology directed repair in somatic plant tissue
CA3183409A1 (en) * 2020-05-29 2021-12-02 Pioneer Hi-Bred International, Inc. Large scale genome manipulation
AU2021338707A1 (en) * 2020-09-10 2023-04-06 Monsanto Technology Llc Increasing gene editing and site-directed integration events utilizing meiotic and germline promoters
US20240002877A1 (en) * 2020-10-21 2024-01-04 Pioneer Hi-Bred International, Inc. Parthenogenesis factors and methods of using same
CN113176263A (zh) * 2021-05-06 2021-07-27 云南省农业科学院粮食作物研究所 利用高倍显微镜辅助鉴定玉米单倍体幼苗植株的方法
CN113151303B (zh) * 2021-05-21 2022-07-05 浙江大学 白菜干细胞决定相关基因BrWUS1及其应用
EP4197318A1 (en) * 2021-12-17 2023-06-21 Albert-Ludwigs-Universität Freiburg Phosphodiesterases inhibitors to promote in vitro plant cell reprogramming towards plant embryogenesis or microcallus formation
WO2023183918A1 (en) * 2022-03-25 2023-09-28 Pioneer Hi-Bred International, Inc. Methods of parthenogenic haploid induction and haploid chromosome doubling
CN115386589A (zh) * 2022-05-09 2022-11-25 上海大学 一种玉米自交系双载体遗传转化系统、其构建方法及其应用
CN117385087A (zh) * 2023-11-27 2024-01-12 北京市农林科学院 与小孢子胚胎发生基因连锁的SNP分子标记Me-5676929及其应用和专用引物

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Also Published As

Publication number Publication date
WO2019075295A1 (en) 2019-04-18
US11447786B2 (en) 2022-09-20
AU2018347545A1 (en) 2020-04-16
US20200263189A1 (en) 2020-08-20
CA3078819A1 (en) 2019-04-18
CN111465321A (zh) 2020-07-28
EP3694308A1 (en) 2020-08-19
MX2020004138A (es) 2020-08-13
IL273710A (en) 2020-05-31
ZA202002527B (en) 2021-05-26
US20230203516A1 (en) 2023-06-29
CN111465321B (zh) 2022-12-06
BR112020007343A2 (pt) 2020-10-06

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