CL2020000814A1 - Biosíntesis heterológica de ácidos nodulispóricos - Google Patents

Biosíntesis heterológica de ácidos nodulispóricos

Info

Publication number
CL2020000814A1
CL2020000814A1 CL2020000814A CL2020000814A CL2020000814A1 CL 2020000814 A1 CL2020000814 A1 CL 2020000814A1 CL 2020000814 A CL2020000814 A CL 2020000814A CL 2020000814 A CL2020000814 A CL 2020000814A CL 2020000814 A1 CL2020000814 A1 CL 2020000814A1
Authority
CL
Chile
Prior art keywords
genes
nas
biosynthesis
pulicicidum
naf
Prior art date
Application number
CL2020000814A
Other languages
English (en)
Inventor
Matthew Joseph Nicholson
Sarah Adeline Kessans
Emily Jane Parker
Rodriguez Leyla Yolanda Bustamante
David Barry Scott
De Bittner Kyle Cornelius Van
Dolleweerd Craig John Van
Original Assignee
Victoria Link Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2017903956A external-priority patent/AU2017903956A0/en
Application filed by Victoria Link Ltd filed Critical Victoria Link Ltd
Publication of CL2020000814A1 publication Critical patent/CL2020000814A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/182—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Virology (AREA)
  • Pest Control & Pesticides (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Los ácidos nodulispóricos (NAs) comprenden un grupo de indol diterpenos conocidos por sus potentes actividades insecticidas; sin embargo, la biosíntesis de los NAs por su productor natural, Hypoxylon pulicicidum (Nodulisporium sp.) es excepcionalmente difícil de lograr. La identificación de genes responsables de la producción de NA podría permitir optimizar la vía biosintética para proporcionar acceso a los NAs para aplicaciones comerciales. La obtención de cantidades útiles de NAs usando los métodos de fermentación publicados constituye un desafío, haciendo que los estudios de inactivación génica sean un método indeseable para confirmar la función génica. Alternativamente, la expresión génica heteróloga de genes de H. pulicicidum en una especie huésped más robusta como Penicillium paxilli proporciona un camino para identificar rápidamente la función de los genes que desempeñan un rol en la biosíntesis de NA. En este trabajo, nosotros identificamos la función de cuatro genes metabólicos secundarios necesarios para la biosíntesis del ácido nodulispórico F (NAF) y reconstituimos estos genes en el genoma de P. paxilli para permitir la producción heteróloga de NAF en este hongo.
CL2020000814A 2017-09-29 2020-03-30 Biosíntesis heterológica de ácidos nodulispóricos CL2020000814A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AU2017903956A AU2017903956A0 (en) 2017-09-29 Heterologous biosynthesis of nodulisporic acid

Publications (1)

Publication Number Publication Date
CL2020000814A1 true CL2020000814A1 (es) 2020-12-18

Family

ID=65901342

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2020000814A CL2020000814A1 (es) 2017-09-29 2020-03-30 Biosíntesis heterológica de ácidos nodulispóricos

Country Status (11)

Country Link
US (2) US11453882B2 (es)
EP (1) EP3688174A4 (es)
JP (1) JP2021500868A (es)
CN (1) CN111542602A (es)
AU (1) AU2018343310B2 (es)
BR (1) BR112020006214A2 (es)
CA (1) CA3076164A1 (es)
CL (1) CL2020000814A1 (es)
MX (1) MX2020003367A (es)
WO (1) WO2019064243A1 (es)
ZA (1) ZA202002050B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114350524B (zh) * 2022-01-07 2024-04-23 暨南大学附属第一医院（广州华侨医院） Mycoleptodiscin型吲哚倍半萜化合物、其生物合成方法及其用途
CN114940980B (zh) * 2022-06-02 2023-11-21 暨南大学 一类倍半萜聚酮合成基因及其应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5399582A (en) 1993-11-01 1995-03-21 Merck & Co., Inc. Antiparasitic agents
SK284840B6 (sk) * 1995-03-20 2005-12-01 Merck & Co., Inc. Deriváty kyseliny nodulisporovej a farmaceutický prostriedok s ich obsahom
US5792294A (en) 1995-11-16 1998-08-11 Otis Elevator Company Method of replacing sheave liner
US20040077703A1 (en) * 2002-10-02 2004-04-22 Soll Mark D. Nodulisporic acid derivative spot-on formulations for combating parasites
NZ530331A (en) * 2003-12-22 2006-09-29 Agres Ltd Nucleic acids and polypeptides for production of an indole diterpene, enzyme, intermediate or other chemical compounds associated with the indole diterpene biosynthetic pathway in grass
ES2729854T3 (es) * 2008-11-14 2019-11-06 Merial Inc Compuestos de ariloazol-2-ilcianoetilamino parasiticidas enriquecidos enantioméricamente

Also Published As

Publication number Publication date
US12065650B2 (en) 2024-08-20
CN111542602A (zh) 2020-08-14
EP3688174A1 (en) 2020-08-05
CA3076164A1 (en) 2019-04-04
EP3688174A4 (en) 2021-09-08
US20230365976A1 (en) 2023-11-16
US11453882B2 (en) 2022-09-27
WO2019064243A1 (en) 2019-04-04
MX2020003367A (es) 2020-07-29
BR112020006214A2 (pt) 2020-12-01
AU2018343310B2 (en) 2023-11-30
JP2021500868A (ja) 2021-01-14
US20200299700A1 (en) 2020-09-24
AU2018343310A1 (en) 2020-04-23
ZA202002050B (en) 2023-08-30

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