CL2020000418A1 - Gen que confiere resistencia frente a patógenos fúngicos. - Google Patents

Gen que confiere resistencia frente a patógenos fúngicos.

Info

Publication number
CL2020000418A1
CL2020000418A1 CL2020000418A CL2020000418A CL2020000418A1 CL 2020000418 A1 CL2020000418 A1 CL 2020000418A1 CL 2020000418 A CL2020000418 A CL 2020000418A CL 2020000418 A CL2020000418 A CL 2020000418A CL 2020000418 A1 CL2020000418 A1 CL 2020000418A1
Authority
CL
Chile
Prior art keywords
gene
resistance against
confers resistance
fungal pathogens
against fungal
Prior art date
Application number
CL2020000418A
Other languages
English (en)
Inventor
Milena Ouzunova
Daniela Scheuermann
Beat Keller
Simon Krattinger
Thomas Wicker
Gerhard Herren
Bettina Kessel
Thomas Presterl
Ping Yang
Original Assignee
Kws Saat Se & Co Kgaa
Univ Zuerich
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kws Saat Se & Co Kgaa, Univ Zuerich filed Critical Kws Saat Se & Co Kgaa
Publication of CL2020000418A1 publication Critical patent/CL2020000418A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A01H3/04—Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/99—Other protein kinases (2.7.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

LA PRESENTE INVENCIÓN ESTÁ RELACIONADA CON UNA MOLÉCULA DE ÁCIDO NUCLEICO QUE CODIFICA UN POLIPÉPTIDO QUE CONFIERE RESISTENCIA A UNA PLANTA FRENTE A UN PATÓGENO FÚNGICO, TAL COMO HELMINTHOSPORIUM TURCICUM. LA PRESENTE INVENCIÓN ADEMÁS ESTÁ RELACIONADA CON UNA PLANTA (O PARTE DE ESTA) QUE COMPRENDE LA MOLÉCULA DE ÁCIDO NUCLEICO, Y MÉTODOS EN LOS QUE INTERVIENE LA MOLÉCULA DE ÁCIDO NUCLEICO.
CL2020000418A 2017-08-22 2020-02-20 Gen que confiere resistencia frente a patógenos fúngicos. CL2020000418A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP17187309.4A EP3447134B1 (en) 2017-08-22 2017-08-22 Increased fungal resistance in crop plants
EP17206305.9A EP3447135A1 (en) 2017-08-22 2017-12-08 Gene conferring resistance to fungal pathogen

Publications (1)

Publication Number Publication Date
CL2020000418A1 true CL2020000418A1 (es) 2020-07-10

Family

ID=59686805

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2020000418A CL2020000418A1 (es) 2017-08-22 2020-02-20 Gen que confiere resistencia frente a patógenos fúngicos.

Country Status (11)

Country Link
US (3) US20200231984A1 (es)
EP (4) EP3447134B1 (es)
CN (2) CN111247243A (es)
AR (2) AR113167A1 (es)
BR (1) BR112020003336A2 (es)
CA (2) CA3073306A1 (es)
CL (1) CL2020000418A1 (es)
EA (1) EA202090540A1 (es)
PH (1) PH12020500361A1 (es)
WO (2) WO2019038326A1 (es)
ZA (1) ZA202001047B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108588087B (zh) * 2018-05-16 2022-06-03 南京农业大学 一种提高植物抗病性的基因GmLecRK-R及其应用
CN110564813A (zh) * 2019-09-25 2019-12-13 青海大学 一种与青稞条纹病相关的miRNAs及其挖掘方法
CN110699357B (zh) * 2019-11-08 2021-02-12 山东农业大学 纹枯病菌微小rna、其对玉米基因的调控作用及其应用
BR112022026128A2 (pt) 2020-07-14 2023-01-24 Kws Saat Se & Co Kgaa Métodos para identificar e selecionar plantas de milho com resistência à mangra da folha do milho do norte
CN112852829B (zh) * 2020-12-19 2022-07-08 河南农业大学 小麦茎基腐病相关基因TaDIR-B1及其应用
CN113403308B (zh) * 2020-12-25 2022-10-21 华南农业大学 一种提高水稻抗白叶枯病的方法
EP4108076A1 (en) 2021-06-22 2022-12-28 KWS SAAT SE & Co. KGaA Enhanced disease resistance of maize to northern corn leaf blight by a qtl on chromosome 4
CN115058533B (zh) * 2022-06-06 2024-05-24 吉林省白城市农业科学院(吉林省向日葵研究所) 用于燕麦茎中基因表达分析的内参基因及应用
CN116024234B (zh) * 2022-12-12 2023-07-21 南京林业大学 一种杨树壳针孢菌效应蛋白SmCSEP3及其应用
CN116406590B (zh) * 2023-03-31 2023-12-22 东北农业大学 一种利用片段化番茄esDNA提高番茄植株抗性、防治灰霉病的方法及应用
CN117413738B (zh) * 2023-12-19 2024-03-22 中国农业科学院农业资源与农业区划研究所 一种用于镉污染稻田土壤的水稻安全生产方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6466398A (en) * 1997-03-13 1998-09-29 Dekalb Genetics Corporation Maize dimboa biosynthesis genes
EP1637607B1 (en) 1998-11-12 2014-04-23 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Chimeric promoters capable of mediating gene expression in plants upon pathogen infection and uses thereof
DE102006029129A1 (de) 2006-06-22 2007-12-27 Kws Saat Ag Pathogen induzierbarer synthetischer Promotor
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
AU2010327998B2 (en) 2009-12-10 2015-11-12 Iowa State University Research Foundation, Inc. TAL effector-mediated DNA modification
MX2012014663A (es) 2010-06-25 2013-01-22 Du Pont Composiciones y metodos para mejorar la resistencia al tizon norteño de las hojas en maiz.
US10897862B2 (en) * 2013-09-04 2021-01-26 KWS SAAT SE & Co. KGaA Plant resistant to Helminthosporium turcicum
AR106430A1 (es) * 2015-10-16 2018-01-17 Pioneer Hi Bred Int Generación de plantas de maíz con resistencia mejorada al tizón norteño de las hojas
CN109844121A (zh) * 2016-10-13 2019-06-04 先锋国际良种公司 产生北方叶枯病抗性玉蜀黍

Also Published As

Publication number Publication date
EP3447134A1 (en) 2019-02-27
AR113167A1 (es) 2020-02-05
US20210071194A1 (en) 2021-03-11
CA3073306A1 (en) 2019-02-28
WO2019038339A1 (en) 2019-02-28
EP3447135A1 (en) 2019-02-27
US20230021982A1 (en) 2023-01-26
PH12020500361A1 (en) 2021-01-25
ZA202001047B (en) 2021-04-28
CA3073467A1 (en) 2019-02-28
CN111247244A (zh) 2020-06-05
US20200231984A1 (en) 2020-07-23
CN111247243A (zh) 2020-06-05
EP3673052A1 (en) 2020-07-01
WO2019038326A1 (en) 2019-02-28
CN111247244B (zh) 2024-04-16
BR112020003336A2 (pt) 2020-08-25
EA202090540A1 (ru) 2020-05-28
EP3673051A1 (en) 2020-07-01
EP3447134B1 (en) 2023-10-11
AR112863A1 (es) 2019-12-26

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